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https://www.readbyqxmd.com/read/29331687/probiotics-bacillus-toyonensis-and-saccharomyces-boulardii-improve-the-vaccine-immune-response-to-bovine-herpesvirus-type-5-in-sheep
#1
Talita Bandeira Roos, Carina Martins de Moraes, Régis Tuchtenhagen Sturbelle, Luana Alves Dummer, Geferson Fischer, Fábio Pereira Leivas Leite
There have been significant efforts toward the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotics Bacillus toyonensis and Saccharomyces boulardii as a potential immune modulator to improve vaccine efficiency. Thirty, 5-month-old lambs were randomly grouped in three lots of 10 each and vaccinated at days 0, 21 and 42 of the experiment...
December 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29320512/killing-wolves-to-prevent-predation-on-livestock-may-protect-one-farm-but-harm-neighbors
#2
Francisco J Santiago-Avila, Ari M Cornman, Adrian Treves
Large carnivores, such as gray wolves, Canis lupus, are difficult to protect in mixed-use landscapes because some people perceive them as dangerous and because they sometimes threaten human property and safety. Governments may respond by killing carnivores in an effort to prevent repeated conflicts or threats, although the functional effectiveness of lethal methods has long been questioned. We evaluated two methods of government intervention following independent events of verified wolf predation on domestic animals (depredation) in the Upper Peninsula of Michigan, USA between 1998-2014, at three spatial scales...
2018: PloS One
https://www.readbyqxmd.com/read/29283514/an-animal-model-of-local-breast-cancer-recurrence-in-the-setting-of-autologous-fat-grafting-for-breast-reconstruction
#3
Wakako Tsuji, Jolene E Valentin, Kacey G Marra, Albert D Donnenberg, Vera S Donnenberg, J Peter Rubin
Autologous fat grafting after breast cancer surgery is commonly performed, but concerns about oncologic risk remain. To model the interaction between fat grafting and breast cancer cells, two approaches were employed. In the first approach, graded numbers of viable MDA-MB-231 or BT-474 cells were admixed directly into human fat grafts and injected subcutaneously into immune-deficient mice to determine if the healing graft is a supportive environment for the tumor. In the second approach, graded doses of MDA-MB-231 cells were suspended in Matrigel and injected into the mammary fat pads of mice...
January 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29233823/large-cardiac-muscle-patches-engineered-from-human-induced-pluripotent-stem-cell-derived-cardiac-cells-improve-recovery-from-myocardial-infarction-in-swine
#4
Ling Gao, Zachery R Gregorich, Wuqiang Zhu, Saidulu Mattapally, Yasin Oduk, Xi Lou, Ramaswamy Kannappan, Anton V Borovjagin, Gregory P Walcott, Andrew E Pollard, Vladimir G Fast, Xinyang Hu, Steven G Lloyd, Ying Ge, Jianyi Zhang
Background -Here, we generated human cardiac muscle patches (hCMPs) of clinically relevant dimensions (4 cm × 2 cm × 1.25 mm) by suspending cardiomyocytes, smooth-muscle cells, and endothelial cells that had been differentiated from human induced-pluripotent stem cells (hiPSCs) in a fibrin scaffold and then culturing the construct on a dynamic (rocking) platform. Methods -In vitro assessments of hCMPs suggest maturation in response to dynamic culture stimulation. In vivo assessments were conducted in a porcine model of myocardial infarction (MI)...
December 12, 2017: Circulation
https://www.readbyqxmd.com/read/29215832/-activation-of-regeneration-of-red-and-white-pulp-of-the-spleen-after-the-combined-transplantation-of-hsc-and-mscs-in-terms-of-exposure-to-ionizing-radiation
#5
I Yu Maklakova, D Yu Grebnev, A P Yastrebov
The purpose of this work was to study the effect of combined transplantation of multipotent mesenchymal stromal (MSCS) and hematopoietic stem cells (HSCs) isolated from the placenta, on the regeneration of white and red pulp of the spleen under physiological conditions and in conditions of exposure to ionizing radiation. Methods. The experiments were performed with laboratory mice-males. We studied the influence of ionizing radiation dose of 4.0 Gy. Animals of the experimental group were intravenously infused into MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, suspended in 0...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
https://www.readbyqxmd.com/read/29166379/bcfa-enriched-vernix-monoacylglycerol-reduces-lps-induced-inflammatory-markers-in-human-enterocytes-in-vitro
#6
Yuanyuan Yan, Zhen Wang, Donghao Wang, Peter Lawrence, Xingguo Wang, Kumar S D Kothapalli, Jacelyn Greenwald, Ruijie Liu, Hui Gyu Park, J Thomas Brenna
BACKGROUND: Excess vernix caseosa produced by the fetal skin appears as particles suspended in the amniotic fluid in late gestation, is swallowed by the fetus and is found throughout the newborn GI tract as the first organisms are arriving to colonize the gut. Lipid rich vernix contains an unusually high 29% branched chain fatty acid (BCFA). BCFA reduce the incidence of necrotizing enterocolitis in an animal model, recently found predominantly in the sn-2 position of human milk triacylglycerols...
November 22, 2017: Pediatric Research
https://www.readbyqxmd.com/read/29166277/the-influence-of-doxazosin-on-the-contractility-of-the-urinary-bladder-in-female-pigs-with-experimentally-induced-cystitis
#7
W Markiewicz, A Bossowska, E Lepiarczyk, M Majewski, P Radziszewski, A Wiśniewska, J J Jaroszewski
The present in vitro study investigated the influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis. Fifteen juvenile female piglets (18-20 kg body weight) were randomly assigned into three groups (n=5 animals each): i) control (clinically healthy animals, without doxazosin treatment), ii) animals with induced inflammation of the urinary bladder, but without doxazosin treatment (experimental group I) and iii) animals with inflamed bladder, treated orally with doxazosin (0...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29165406/-the-significance-of-fibroblasts-in-experimental-modeling-of-proliferative-vitreoretinopathy
#8
I P Khoroshilova-Maslova, N L Leparskaya, E A Vorotelyak, A V Vasiliev
AIM: to investigate the role of heterogeneous fibroblasts in the development of epiretinal membrane in eyes with modeled proliferative vitreoretinopathy. MATERIAL AND METHODS: The material for investigation were 6 eyes of 3 Chinchilla rabbits. Suspended fibroblasts (fibroblasts of the human skin - 200000 cells in 0.1 ml) were injected into the vitreous cavity via the pars plana. The animals were followed up for 1 month and then made out of the experiment. The eyes were enucleated and fixed in 10% neutral buffered formalin for routine histological examination...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/29158222/trehalose-metabolism-genes-render-rice-white-tip-nematode-aphelenchoides-besseyi-nematoda-aphelenchoididae-resistant-to-anaerobic-environment
#9
Qiaoli Chen, Feng Wang, Danlei Li, Ruizhi Zhang, Yaming Ling
After experiencing anaerobic environments, Aphelenchoides besseyi will enter a state of suspended animation known as anoxybiosis, which may use trehalose for energy supply to survive. To identify the function of trehalose metabolism, two trehalose-6-phosphate synthase (TPS) genes (Ab-tps1 and Ab-tps2) encoding enzymes catalyzing trehalose synthesis and three trehalase (TRE) genes (Ab-ntre1, Ab-ntre2 and Ab-atre) encoding enzymes catalyzing the hydrolysis of trehalose were identified and investigated. Ab-tps1 and Ab-tps2 were active when A...
November 20, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29150890/suspended-animation-the-past-present-and-future-of-major-cardiothoracic-trauma
#10
REVIEW
Syed Mohiyaddin, Prakash Nanjaiah, Ahmed O Saad, Metesh N Acharya, Tanveer A Khan, Rhodri H Davies, Saeed Ashraf
About 50% of the trauma victims die at the scene mostly because of exsanguinating haemorrhage. Most trials of resuscitation fail in face of the ongoing bleeding. Ongoing research/studies to save these victims by inducing rapid hypothermia using cardiopulmonary bypass as an emergency initial measure along with delayed resuscitation show improved outcomes. A comprehensive review of this research and analysis of studies showed that rapid induction of hypothermia within 5 min of cardiac arrest is associated with better survival and improved neurological outcome...
November 18, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/29115131/cultivation-of-the-ribbed-mussel-geukensia-demissa-for-nutrient-bioextraction-in-an-urban-estuary
#11
Eve Galimany, Gary H Wikfors, Mark S Dixon, Carter R Newell, Shannon L Meseck, Dawn Henning, Yaqin Li, Julie M Rose
Shellfish aquaculture is gaining acceptance as a tool to reduce nutrient over enrichment in coastal and estuarine ecosystems through the feeding activity of the animals and assimilation of filtered particles in shellfish tissues. This ecosystem service, provided by the ribbed mussel (Geukensia demissa), was studied in animals suspended from a commercial mussel raft in the urban Bronx River Estuary, NY, in waters closed to shellfish harvest due to bacterial contamination. Naturally occurring populations of ribbed mussels were observed to be healthy and resilient in this highly urbanized environment...
November 8, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29112150/fabrication-of-in-vitro-cancer-microtissue-array-on-fibroblast-layered-nanofibrous-membrane-by-inkjet-printing
#12
Tae-Min Park, Donggu Kang, Ilho Jang, Won-Soo Yun, Jin-Hyung Shim, Young Hun Jeong, Jong-Young Kwak, Sik Yoon, Songwan Jin
In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing...
November 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29111563/multiple-genes-contribute-to-anhydrobiosis-tolerance-to-extreme-desiccation-in-the-nematode-panagrolaimus-superbus
#13
Cláudia Carolina Silva Evangelista, Giovanna Vieira Guidelli, Gustavo Borges, Thais Fenz Araujo, Tiago Alves Jorge de Souza, Ubiraci Pereira da Costa Neves, Alan Tunnacliffe, Tiago Campos Pereira
The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways...
November 6, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29107204/laser-photobiomodulation-of-pro-inflammatory-mediators-on-walker-tumor-256-induced-rats
#14
Maria Carla Petrellis, Lúcio Frigo, Rodrigo Labat Marcos, Rodney Capp Pallotta, Maria Helena Catelli de Carvalho, Marcelo Nicolás Muscará, Durvanei Augusto Maria, Rodrigo Álvaro Brandão Lopes-Martins
Laser photobiomodulation or low-level laser therapy (LLLT) is recognized worldwide for its expansive use in medicine. LLLT has been reported to increase enzymatic activity, increasing the mitochondrial transmembrane potential, leading to an increased energy availability and signal transduction. Nevertheless, an inhibitory effect is also observed by the production of excessive ROS which can result the shutdown of mitochondrial energy production, and finally to apoptosis. However, the mechanism of apoptosis induced by LLLT is still not well understood...
December 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29103865/human-endothelial-cell-collection-from-the-middle-cerebral-artery-in-acute-ischemic-stroke
#15
Sunil A Sheth, Abhishek Verma, Konark Malhotra, Xiannan Tang, Satoshi Tateshima, Reza Jahan, Jason D Hinman
BACKGROUND: Endovascular treatment for large-vessel acute ischemic stroke (AIS) has rapidly emerged. However, the understanding of the complex biology involving endothelial cells (ECs) remains scarce. METHODS: Using stent retrievers during endovascular thrombectomy (ET) in patients with AIS, ECs were segregated, centrifuged in a dissociation buffer, and suspended in endothelial specific antibody solution. Subsequently, fluorescence-activated cell sorting (FACS) and microscopic analyses were performed...
November 2, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/29066414/z-505-hydrochloride-ameliorates-chemotherapy-induced-anorexia-in-rodents-via-activation-of-the-ghrelin-receptor-ghsr1a
#16
Yoshihiro Shiomi, Yuta Ohira, Makoto Yoshimura, Tomoko Ozaki, Mineo Takei, Takao Tanaka
Despite its therapeutic advantages, chemotherapy with anti-cancer drugs can cause adverse effects, including anorexia and weight loss. Although most patients with cancer suffer from anorexia during chemotherapy, resulting in the need to suspend or cease treatment and thereby worsening prognosis, treatment options for anorexia remain limited. Ghrelin is an orexigenic hormone that has been proposed to prevent anorexia. To investigate the potential of ghrelin receptor agonists, synthetic small-molecule compounds, as preventive therapies for chemotherapy-induced anorexia, we studied the effects of Z-505 hydrochloride (Z-505), a new oral growth hormone secretagogue receptor 1a (GHSR1a) agonist, in cisplatin- and 5-fluorouracil (5-FU)-induced anorexia animal models...
October 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29048868/control-of-carbon-nanotube-solvatochromic-response-to-chemotherapeutic-agents
#17
Jackson D Harvey, Hanan A Baker, Elizabeth Mercer, Januka Budhathoki-Uprety, Daniel A Heller
Alkylating agents such as cisplatin play an essential role in chemotherapy regimens, but initial and acquired resistance in many cancer types often dampen therapeutic response. The poor understanding of the mechanisms of resistance highlight the need for quantitative measurements of alkylating agent distribution at both the tissue and subcellular levels. Sensors for use in live animals and cells would allow for more effective study of drug action and resistance. Toward this end, single-walled carbon nanotubes suspended with single-stranded DNA have suitable optical properties for in vivo sensors, such as near-infrared emission and sensitivity to the local environment via solvatochromic responses...
October 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29025328/safety-assessment-of-cross-linked-alkyl-acrylates-as-used-in-cosmetics
#18
Monice M Fiume, Bart Heldreth, Ivan Boyer, Wilma F Bergfeld, Donald V Belsito, Ronald A Hill, Curtis D Klaassen, Daniel C Liebler, James G Marks, Ronald C Shank, Thomas J Slaga, Paul W Snyder, F Alan Andersen
The Cosmetic Ingredient Review (CIR) Expert Panel assessed the safety of cross-linked alkyl acrylates as used in cosmetics. The 23 cross-linked alkyl acrylates included in this safety assessment are reported to function as absorbents, film formers, emulsion stabilizers, viscosity increasing agents, suspending agents, binders, and/or skin-conditioning agents. The Panel reviewed available animal and clinical data, as well as information from previous CIR reports on monomer components. Because data were not available for the individual ingredients, and because residual monomer may be present, the Panel extrapolated from previous reports to support safety...
September 2017: International Journal of Toxicology
https://www.readbyqxmd.com/read/28979243/phenotypic-and-transcriptomic-responses-of-campylobacter-jejuni-suspended-in-an-artificial-freshwater-medium
#19
Hana Trigui, Kristen Lee, Alexandre Thibodeau, Simon Lévesque, Nilmini Mendis, Philippe Fravalo, Ann Letellier, Sébastien P Faucher
Campylobacter jejuni is the leading cause of campylobacteriosis in the developed world. Although most cases are caused by consumption of contaminated meat, a significant proportion is linked to ingestion of contaminated water. The differences between C. jejuni strains originating from food products and those isolated from water are poorly understood. Working under the hypothesis that water-borne C. jejuni strains are better equipped at surviving the nutrient-poor aquatic environment than food-borne strains, the present study aims to characterize these differences using outbreak strains 81116 and 81-176...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28974933/gaseous-mediators-and-mitochondrial-function-the-future-of-pharmacologically-induced-suspended-animation
#20
REVIEW
Clair Hartmann, Benedikt Nussbaum, Enrico Calzia, Peter Radermacher, Martin Wepler
The role of nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S) as poisonous gases is well-established. However, they are not only endogenously produced but also, at low concentrations, exert beneficial effects, such as anti-inflammation, and cytoprotection. This knowledge initiated the ongoing debate, as to whether these molecules, also referred to as "gaseous mediators" or "gasotransmitters," could serve as novel therapeutic agents. In this context, it is noteworthy, that all gasotransmitters specifically target the mitochondria, and that this interaction may modulate mitochondrial bioenergetics, thereby subsequently affecting metabolic function...
2017: Frontiers in Physiology
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