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G P Adams, M H Ratto, M E Silva, R A Carrasco
The ovulation-inducing effect of seminal plasma was first reported in Bactrian camels over 30 years ago, and the entity responsible was dubbed 'ovulation-inducing factor' (OIF). More recent studies, primarily in llamas and alpacas, characterized the biological and chemical properties of OIF and ultimately identified it as βNGF. This recent discovery has allowed a convergence of knowledge previously separated by discipline and by mechanism; that is, neurobiology and reproductive biology, and autocrine/paracrine vs endocrine...
October 2016: Reproduction in Domestic Animals, Zuchthygiene
Carl Savage, Louise Parke, Mia von Knorring, Pamela Mazzocato
BACKGROUND: Health care has experimented with many different quality improvement (QI) approaches with greater variation in name than content. This has been dubbed pseudoinnovation. However, it could also be that the subtleties and differences are not clearly understood. To explore this further, the purpose of this study was to explore how hospital managers perceive lean in the context of QI. METHODS: We used a qualitative study design with semi-structured interviews to explore twelve top managers' perceptions of the relationship between lean and quality improvement (QI) at a university-affiliated hospital...
October 19, 2016: BMC Health Services Research
Samuel D Whedon, Nagula Markandeya, Ambar S J B Rana, Caroline E Weller, Nicholas A Senger, Frantisek Turecek, Eric R Strieter, Champak Chatterjee
Deubiquitylating enzymes (DUBs) remove ubiquitin (Ub) from various cellular proteins and render eukaryotic ubiquitylation a dynamic process. The misregulation of protein ubiquitylation is associated with many human diseases, and there is an urgent need to identify specific DUBs associated with therapeutically relevant targets of Ub. We report the development of two facile selenocysteine-based strategies to generate the DUB probe dehydroalanine (Dha). Optimized oxidative or alkylative elimination of Se yielded Dha at the C-terminus of Ub...
October 10, 2016: Journal of the American Chemical Society
P Ginod, B Khallouk, E Benzenine, T Desplanches, T Dub, E Schmutz, S Douvier, P Sagot
OBJECTIVES: To analyse episiotomy and perineal tears rates in Burgundy after French National College of Obstetricians and Gynecologists (CNGOF) guidelines in 2005. MATERIALS AND METHOD: Multicenter retrospective study, between 2003-2005 (period 1) et 2012-2014 (period 2), conducted on singletons vaginal deliveries, in cephalic presentation from 37 weeks. We compared the episiotomy rate (ER), and perineal lesions in normal deliveries (ND) and instrumental deliveries (ID) between the two periods...
October 6, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
Simon Hauri, Federico Comoglio, Makiko Seimiya, Moritz Gerstung, Timo Glatter, Klaus Hansen, Ruedi Aebersold, Renato Paro, Matthias Gstaiger, Christian Beisel
Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Here, we systematically mapped the human PcG complexome using a robust affinity purification mass spectrometry approach. Our high-density protein interaction network uncovered a diverse range of PcG complexes. Moreover, our analysis identified PcG interactors linking them to the PcG system, thus providing insight into the molecular function of PcG complexes and mechanisms of recruitment to target genes...
October 4, 2016: Cell Reports
Johnathan Neiswinger, Ijeoma Uzoma, Eric Cox, HeeSool Rho, Guang Song, Corry Paul, Jun Seop Jeong, Kuan-Yi Lu, Chien-Sheng Chen, Heng Zhu
Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the "yeast proteome array," consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein-lipid interactions. Further assays were subsequently developed to allow measurement of protein-DNA, protein-RNA, and protein-protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation...
October 3, 2016: Cold Spring Harbor Protocols
Igor Demchenko, Reuven Katz, Hillel Pratt, Miriam Zacksenhouse
Discrepancies between actual and appropriate motor commands, dubbed low-level errors, have been shown to elicit a P300 like component. P300 has been studied extensively in cognitive tasks using, in particular, the three-stimulus oddball paradigm. This paradigm revealed two sub-components, known as P3a and P3b, whose relative contributions depend on saliency and task-relevance, respectively. However, the existence and roles of these sub-components in response to low-level errors are poorly understood. Here we investigated responses to low level errors generated by disturbances - including target and cursor jumps, versus responses to distractors, i...
September 28, 2016: Brain Research
Asle H Kiran
The changes that happen to healthcare services after the implementation of new assistive healthcare technologies (also called 'welfare technology' in the Nordic countries) concern more than increased efficiency and reducing healthcare expenditure. Of particular interest from an ethical point of view are the manners in which technologies shape the roles and identities of care receivers and healthcare personnel. The notion of 'patienthood' is explored in this paper as something that is both challenged by new technologies, and as something that is opened up for active and potentially positive reshaping when care receivers support their illness or frailty with assistive healthcare technologies...
September 29, 2016: Nursing Philosophy: An International Journal for Healthcare Professionals
Maria Stancescu, Tatiana A Fedotova, Jef Hooyberghs, Alexander Balaeff, Dmitry M Kolpashchikov
Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 °C) and in narrow intervals (ΔT = 1-10 °C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 °C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion"...
October 4, 2016: Journal of the American Chemical Society
Jon Scott Stevens, Lila R Gleitman, John C Trueswell, Charles Yang
We evaluate here the performance of four models of cross-situational word learning: two global models, which extract and retain multiple referential alternatives from each word occurrence; and two local models, which extract just a single referent from each occurrence. One of these local models, dubbed Pursuit, uses an associative learning mechanism to estimate word-referent probability but pursues and tests the best referent-meaning at any given time. Pursuit is found to perform as well as global models under many conditions extracted from naturalistic corpora of parent-child interactions, even though the model maintains far less information than global models...
September 25, 2016: Cognitive Science
Mahdi Abbasian, Hadieh Alsadat Eslampanah Seyedi, Badraldin Ebrahim Sayed Tabatabaei, Zahra Arab-Bafrani, Mohammad Reza Mofid, Reza Zareie
Vessel dilator is a 3.9-KDa potent anticancer peptide and a valuable candidate in the treatment of conditions such as congestive heart failure and acute renal failure amongst others. Here we report the recombinant production of vessel dilator in Escherichia coli. Three different synthetic ORF's dubbed VDI, VDII and VDIII, each encoding a trimmer of the vessel dilator peptide attached to a His tag sequence at their C- terminal, were synthesized and placed in pET21c expression vectors. The highest yield, following expression in E...
January 2017: Protein Expression and Purification
Archana D Rathod, Rohidas P Chavan, Sandhya P Pajai, Vijay Bhagat, Prachi Thool
ABSTRACT: Adolescence is a period of enormous physical and psychological change for young girls. Many adolescents with menstrual disturbances never present to their family doctor or gynecologist. Embarrassment about discussing menstruation, fear of disease, and ignorance about services available may lead to delayed presentation or consultation with doctor. AIMS AND OBJECTIVE: To evaluate the different gynecological problems in adolescent girls attending outpatient department...
October 2016: Journal of Obstetrics and Gynaecology of India
Anand Murari Nanavati, Sudhir B Gokral
PURPOSE: The present study is a prospective, randomized comparative study of abdominal, vaginal and laparoscopic hysterectomies comparing various parameters such as indications, blood loss, post operative pain, time taken for surgery, intraoperative and postoperative complications and total hospital stay. MATERIALS AND METHODS: 50 patients were selected in each group over a period of 18 months. The patients were selected in such a way that they could be operated by all 3 routes...
October 2016: Journal of Obstetrics and Gynaecology of India
Shreya Dharadhar, Marcello Clerici, Willem J van Dijk, Alexander Fish, Titia K Sixma
Regulation of deubiquitinating enzyme (DUB) activity is an essential step for proper function of cellular ubiquitin signals. UAF1 is a WD40 repeat protein, which binds and activates three important DUBs, USP1, USP12 and USP46. Here, we report the crystal structure of the USP12-Ub/UAF1 complex at a resolution of 2.8Å and of UAF1 at 2.3Å. In the complex we find two potential sites for UAF1 binding, analogous to what was seen in a USP46/UAF1 complex. In line with these observed dual binding states, we show here that USP12/UAF1 complex has 1:2 stoichiometry in solution, with a two-step binding at 4nM and 325nM respectively...
September 17, 2016: Journal of Structural Biology
Fei-Ye Li, Yao-Dong Li, Yong Baek Kim, Leon Balents, Yue Yu, Gang Chen
Frustrated quantum magnets not only provide exotic ground states and unusual magnetic structures, but also support unconventional excitations in many cases. Using a physically relevant spin model for a breathing pyrochlore lattice, we discuss the presence of topological linear band crossings of magnons in antiferromagnets. These are the analogues of Weyl fermions in electronic systems, which we dub Weyl magnons. The bulk Weyl magnon implies the presence of chiral magnon surface states forming arcs at finite energy...
September 21, 2016: Nature Communications
Lili Xie, Aihong Li, Jiabing Shen, Maohong Cao, Xiaojin Ning, Debin Yuan, Yuteng Ji, Hongmei Wang, Kaifu Ke
OTUB1 is a member of deubiquitinating enzymes, which was shown as a proteasome-associated DUB to be involved in the proteins Ub-dependent degradation. Previous studies have indicated that OTUB1 was expressed in brain. But its distribution and function in the brain remain unclear. In this study, we explored the roles of OTUB1 protein in the pathophysiology of intracerebral hemorrhage (ICH). From the results of Western blot, immunohistochemistry, and immunofluorescence, we found an obvious up-regulation of OTUB1 in neurons adjacent to the hematoma after ICH...
November 2016: Molecular and Cellular Biochemistry
Jin Zhang, Danjie Zhang, Liangzhang Sun
Ubiquitin-specific protease 14 (USP14), one of three proteasome-associated deubiquitinating enzymes (DUBs), plays an essential role in the development of human carcinoma. However, to the best of our knowledge, the role of USP14 in esophageal squamous cell carcinoma (ESCC) is unknown. In the current study, we investigated the expression and role of USP14 in ESCC. Our results showed that the level of USP14 was significantly increased in ESCC tissues and cell lines. Down-regulation of USP14 significantly inhibited ESCC cell proliferation and ESCC tumor growth in nude mice...
September 13, 2016: Oncology Research
Karissa L Paquin, David A Vierra, Niall G Howlett
No abstract text is available yet for this article.
September 14, 2016: Cell Cycle
Edouard Boex-Fontvieille, Sachin Rustgi, Diter von Wettstein, Stephan Pollmann, Steffen Reinbothe, Christiane Reinbothe
A small family of Kunitz protease inhibitors exists in Arabidopsis thaliana, a member of which (encoded by At1g72290) accomplishes highly specific roles during plant development. Arabidopsis Kunitz-protease inhibitor 1 (Kunitz-PI;1), as we dubbed this protein here, is operative as cysteine PI. Activity measurements revealed that despite the presence of the conserved Kunitz-motif the bacterially expressed Kunitz-PI;1 was unable to inhibit serine proteases such as trypsin and chymotrypsin, but very efficiently inhibited the cysteine protease RESPONSIVE TO DESICCATION 21...
2016: Frontiers in Plant Science
Claire Heride, Daniel J Rigden, Erithelgi Bertsoulaki, Danilo Cucchi, Enrico De Smaele, Michael J Clague, Sylvie Urbé
USP21 is a centrosome-associated deubiquitylase (DUB) that has been implicated in the formation of primary cilia, critical organelles for the regulation of the Hedgehog (Hh) signaling pathway in vertebrates. Here we identify KCTD6, a Cullin3 E3-ligase substrate adapter previously linked to Hh-signaling, as well as Gli1, the key transcription factor responsible for Hh signal amplification, as novel interacting partners of USP21. We identify a cryptic structured protein interaction domain in KCTD6, which is predicted to bear fold similarity to Smr domains...
September 12, 2016: Journal of Cell Science
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