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https://www.readbyqxmd.com/read/29039212/propagation-and-titration-of-infectious-bursal-disease-virus-including-non-cell-culture-adapted-strains-using-ex-vivo-stimulated-chicken-bursal-cells
#1
Sebastien Mathieu Soubies, Céline Courtillon, Mouna Abed, Michel Amelot, Alassane Keita, Andrew Broadbent, Sonja Härtle, Bernd Kaspers, Nicolas Eterradossi
Infectious bursal disease virus (IBDV) is a Birnaviridae family member of economic importance for poultry. This virus infects and destroys developing B lymphocytes in the cloacal bursa, resulting in a potentially fatal or immunosuppressive disease in chickens. Naturally-occurring viruses and many vaccine strains are not able to grow in in vitro systems without prior adaptation, which process often affects viral properties such as virulence. Primary bursal cells, which are the main target cells of lymphotropic IBDV in vivo, may represent an attractive system for the study of IBDV...
October 17, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29035864/redescription-of-the-nematode-parasites-of-lizards-strongyluris-oscari-travassos-1923-heterakidae-from-brazil-and-pharyngodon-mamillatus-linstow-1897-pharyngodonidae-from-egypt
#2
Felipe B Pereira, José L Luque, Luiz E R Tavares
Strongyluris oscari Travassos, 1923, parasite of Topidurus torquatus (Wied-Neuwied, 1820) (Tropiduridae) from Brazil and Pharyngodon mamillatus (Linstow, 1897) of Chalcides ocellatus (Forskal, 1775) (Scincidae) from Egypt, were redescribed based on light and scanning electron microscopy, and re-evaluation of specimens from parasitological collections. The following features were accurately observed in S. oscari for the first time: structure of lips and pharyngeal valve, presence and distribution of somatic papillae, number and arrangement of caudal papillae, presence of inner sclerotised structures supporting the ventral sucker and the ventral region in tail of males, and structure of the posterior anal lip in females...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035136/functional-identification-of-allograft-inflammatory-factor-1-like-gene-in-luning-chicken
#3
Yan-Ying Zhao, Ya-Qiu Lin, Ya-Ou Xu
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca(2+) binding EF hand and could interact with actin filament...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29032345/characterization-of-titanyl-phthalocyanine-tiopc-thin-films-by-microscopic-and-spectroscopic-method
#4
R Skonieczny, J Makowiecki, B Bursa, A Krzykowski, M Szybowicz
The titanyl phthalocyanine (TiOPc) thin film deposited on glass, silicon and gold substrate have been studied using Raman spectroscopy, atomic force microscopy (AFM), absorption and profilometry measurements. The TiOPc thin layers have been deposited at room temperature by the quasi-molecular beam evaporation technique. The Raman spectra have been recorded using micro Raman system equipped with a confocal microscope. Using surface Raman mapping techni que with polarized Raman spectra the polymorphic forms of the TiOPc thin films distribution have been obtained...
October 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29027573/dose-reduction-for-fluoroscopically-guided-injections-phantom-simulation-and-patient-procedures
#5
C Y Chang, F J Simeone, M C DeLorenzo, W E Palmer, M A Bredella, A J Huang
OBJECTIVE: To demonstrate fluoroscopy dose reduction through both simulated injections on a phantom and patient injections. MATERIALS AND METHODS: Our study was IRB-approved and HIPAA-compliant. Simulation on a phantom was used to estimate effective dose, entrance dose, and organ doses for hip joint injections without and with dose minimization technique (DMT). Additionally, 1,094 joint, bursae, and tendon sheath injections performed by three operators in the same fluoroscopy suite were evaluated both before and after application of DMT...
October 13, 2017: Skeletal Radiology
https://www.readbyqxmd.com/read/29018431/depression-of-vaccinal-immunity-to-marek-s-disease-by-infection-with-chicken-infectious-anemia-virus
#6
Yankun Zhang, Ning Cui, Ni Han, Jiayan Wu, Zhizhong Cui, Shuai Su
Marek's disease (MD) has been occurring with increasing frequency in chickens in recent years. To our knowledge, however, there has been no report of the very virulent plus (vv+) MD virus (MDV) field isolate in China. Studies have shown that dual infection with immunosuppressive viruses such as chicken infectious anemia virus (CIAV) occurs frequently in chickens developing MD. In this study, we performed a designed set of in vivo experiments, which comprised five different groups of chickens, including the group of CVI988/Rispens-vaccinated chickens, the groups of CVI988/Rispens-vaccinated chickens infected with MDV or CIAV or both viruses (MDV and CIAV), and the group of MDV-challenged chickens...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29018364/cloning-and-functional-characterization-of-oct%C3%AE-2-receptor-and-tyr1-receptor-in-the-chagas-disease-vector-rhodnius-prolixus
#7
Sam Hana, Angela B Lange
Octopamine and tyramine, both biogenic amines, are bioactive chemicals important in diverse physiological processes in invertebrates. In insects, octopamine and tyramine operate analogously to epinephrine and norepinephrine in the vertebrates. Octopamine and tyramine bind to G-protein coupled receptors (GPCRs) leading to changes in second messenger levels and thereby modifying the function in target tissues and insect behavior. In this paper, we report the cDNA sequences of two GPCRs, RhoprOctβ2-R, and RhoprTyr1-R, have been cloned and functionally characterized from Rhodnius prolixus...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28992949/sh3-dependent-cell-death-signaling-of-the-avian-chb6-alloantigen
#8
Rohini Bhattacharya, Cristian Ovies, Deisi Williamson, Sarah Mitchell, Phillip E Funk
In chickens, B cells develop in the bursa of Fabricius, a unique organ for B cell development. Most B cells will die within the bursa, mirroring cell losses seen in mammalian bone marrow as central tolerance is enforced at the transition to mature cells. B cell responses are shaped by a complex interplay of signals. Signals in addition to BCR that impact central tolerance have recently been described. We have been interested in chB6, a novel alloantigen on B cells in the chicken. chB6 is found in close proximity to the BCR and can trigger apoptosis after cross-linking by antibody...
September 28, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28980209/la-sota-vaccination-may-not-protect-against-virus-shedding-and-the-lesions-of-velogenic-newcastle-disease-in-commercial-turkeys
#9
ObianujuNkiruka Okoroafor, Paul Chekwube Eze, Wilfred Sunday Ezema, Chika Nwosu, Christian Okorie-Kanu, P C Animoke, Boniface Anene, John Osita Arinze Okoye
The aim of this project is to study the clinical signs and lesion of velogenic Newcastle disease (vND) in commercial turkeys, and also to find out if La Sota vaccination offered protection against these signs and lesions. The cockerels were included as positive controls. One hundred and twenty turkey poults and cockerels were divided into eight groups as follows: unvaccinated unchallenged turkeys (UUT), unvaccinated challenged turkeys (UCT), vaccinated unchallenged turkeys (VUT), vaccinated challenged turkeys (VCT), and along the same lines, the cockerel groups were UUC, UCC, VUC and vaccinated challenged cockerels (VCC)...
October 5, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28972403/detection-of-different-genotypes-of-infectious-bronchitis-virus-and-of-infectious-bursal-disease-virus-in-european-broilers-during-an-epidemiological-study-in-2013-and-the-consequences-for-the-diagnostic-approach
#10
J J de Wit, C Cazaban, R Dijkman, G Ramon, Y Gardin
To gather recent data regarding the infectious bronchitis (IB) and infectious bursal disease (IBD) situation in Europe, a large-scale field epidemiological survey using diagnostic samples has been implemented in 2013 for about six months in several European countries: France, Germany, Greece, Italy, the Netherlands, Poland, Portugal, the Republic of Ireland, Spain and the United Kingdom. In 234 flocks that were sampled, strains from 10 different IBV genotypes were detected: the 793B genotype was detected most frequently, followed by QX, Massachusetts (Mass) and the Xindadi-like strains...
October 3, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28969942/duck-stimulator-of-interferon-genes-plays-an-important-role-in-host-anti-duck-plague-virus-infection-through-an-ifn-dependent-signalling-pathway
#11
Shun Chen, Zhen Wu, Jinyue Zhang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xinxin Zhao, Anchun Cheng
The human stimulator of interferon gene (STING) is an important molecule in innate immunity that stimulates type I interferon (IFN) production. However, the role of duck STING (duSTING) in innate immunity has yet to be explained. In this study, the full length of the duSTING cDNA sequence (1149bp), which encodes 382 amino acid (aa) residues, was reported and showed the highest sequence similarity with chicken STINGs. The phylogenetic analysis based on STING aa showed that duSTING was grouped onto the birds clade...
September 30, 2017: Cytokine
https://www.readbyqxmd.com/read/28968545/normative-size-of-the-osseous-part-of-calcaneal-bursa-and-its-comparison-with-other-calcaneal-articular-areas
#12
Niladri Kumar Mahato
The retro-calcaneal bursa presents a synovial and a non-synovial osseous part of variable dimensions. Studies objectively measuring the variability of the size of this osseous bursal surface cannot be found in literature. The objective of this study was to investigate (i) the dimension variability of the bony part of the bursa and (ii) the relationship of this surface to other articulating areas of the calcaneus. A digital planimeter was used to measure the bursae (n=86) and other articular surface areas of the calcaneus and statistically compared with ANOVA and correlation estimations...
September 7, 2017: Foot
https://www.readbyqxmd.com/read/28964767/the-angiotensin-converting-enzyme-ace-inhibitor-captopril-disrupts-the-motility-activation-of-sperm-from-the-silkworm-bombyx-mori
#13
Sumiharu Nagaoka, Saori Kawasaki, Hideki Kawasaki, Kaeko Kamei
Angiotensin I-converting enzyme (also known as peptidyl dicarboxypeptidase A, ACE, and EC 3.4.15.1), which is found in a wide range of organisms, cleaves C-terminal dipeptides from relatively short oligopeptides. Mammalian ACE plays an important role in the regulation of blood pressure. However, the precise physiological functions of insect ACE homologs have not been understood. As part of our effort to elucidate new physiological roles of insect ACE, we herein report a soluble ACE protein in male reproductive secretions from the silkmoth, Bombyx mori...
September 28, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28958791/tick-borne-bacteria-and-protozoa-detected-in-ticks-collected-from-domestic-animals-and-wildlife-in-central-and-southern-portugal
#14
André Pereira, Ricardo Parreira, António José Cotão, Mónica Nunes, Maria Luísa Vieira, Fábia Azevedo, Lenea Campino, Carla Maia
Ticks are vectors of many human and animal pathogens. The aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern Portugal. A total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). Overall, the analysis of DNA extracts prepared from ticks collected from hosts of 11 different species in the districts of Castelo Branco, Portalegre, Lisboa, Setúbal, Beja and Faro, revealed the presence of genomic sequences from Anaplasma sp...
September 20, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28956720/applying-fundal-pressure-in-the-second-stage-of-labour-and-its-impact-on-mother-and-infant-health
#15
Semra Pinar, Zekiye Karaçam
We examine the prevalence of the practice of applying fundal pressure in the second stage of labour, the technique used, and its impact on mother and infant health. This analytical cross-sectional study was conducted in a state hospital in the city of Bursa, Turkey with 350 women giving vaginal birth in gestational weeks 37-42. We found that fundal pressure is commonly applied but that this is done without a specific indication, that it is more associated with the performance of an episiotomy, and at the same time, the use of the technique does not have an adverse impact on maternal satisfaction or the health of the mother and infant...
September 28, 2017: Health Care for Women International
https://www.readbyqxmd.com/read/28942459/novel-and-major-qtl-for-branch-angle-detected-by-using-dh-population-from-an-exotic-introgression-in-rapeseed-brassica-napus-l
#16
Yusen Shen, Yi Yang, Ensheng Xu, Xianhong Ge, Yang Xiang, Zaiyun Li
A high-density SNP map was constructed and several novel QTL for branch angle across six environments in Brassica napus were identified. Branch angle is a major determinant for the ideotype of a plant, while the mechanisms underlying this trait in Brassica napus remain elusive. Herein, we developed one doubled haploid population from a cross involving one Capsella bursa-pastoris derived B. napus intertribal introgression line with the compressed branches and wooden stems, and constructed a high-density SNP map covering the genetic distance of 2242...
September 23, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28920177/molecular-detection-of-crimean-congo-hemorrhagic-fever-virus-in-ticks-greece-2012-2014
#17
Anna Papa, Anastasia Kontana, Katerina Tsioka, Ilias Chaligiannis, Smaragda Sotiraki
Crimean-Congo hemorrhagic fever virus (CCHFV) is transmitted to humans mainly through the bite of infected ticks. In Greece, only one clinical case has been observed, in 2008, but the seroprevalence in humans is relatively high (4.2%). To have a first insight into the circulation of CCHFV in Greece, 2000 ticks collected from livestock during 2012-2014 were tested. CCHFV was detected in 36 of the 1290 (2.8%) tick pools (1-5 ticks per pool). Two genetic lineages were identified: Europe 1 and Europe 2. Most Europe 1 sequences were obtained from Rhipicephalus sanguineus sensu lato ticks, while most Europe 2 sequences were recovered from Rhipicephalus bursa ticks...
September 17, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28918072/development-of-a-duplex-semi-nested-pcr-assay-for-detection-of-classical-goose-parvovirus-and-novel-goose-parvovirus-related-virus-in-sick-or-dead-ducks-with-short-beak-and-dwarfism-syndrome
#18
Pengfei Li, Ruihua Zhang, Junhao Chen, Dapeng Sun, Jingjing Lan, Shaoli Lin, Shasha Song, Zhijing Xie, Shijin Jiang
Duck short beak and dwarfism syndrome (SBDS) is an emerging infectious disease caused by a novel goose parvovirus-related virus (NGPV) in China. Until now, it remains uncertain whether the Cherry Valley ducks and mule ducks with SBDS are co-infected with classical goose parvovirus (GPV) and NGPV. In this study, a duplex semi-nested PCR assay with high specificity and sensitivity was developed for detection of the two viruses. Using the duplex PCR assay, NGPV was tested positive in all the 15 duck flocks with SBDS, whereas classical GPV was not detected in all the 133 sick and dead ducks collected from East China...
November 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28914464/expression-of-caveolin-1-in-the-interfollicular-but-not-the-follicle-associated-epithelial-cells-in-the-bursa-of-fabricius-of-chickens
#19
Ildikó Bódi, Krisztina Minkó, Orsolya Fölker, Zsófia Benyeda, Balázs Felföldi, Attila Magyar, Anna Kiss, Vilmos Palya, Imre Oláh
The surface epithelium of the bursa of Fabricius consists of interfollicular (IFE) and follicle-associated epithelium (FAE). The IFE comprises (i) cylindrical-shaped secretory cells (SC) and (ii) cuboidal basal cells (BCs). The FAE provides histological and two-way functional connections between the bursal lumen and medulla of the follicle. We used a carbon solution and anti-caveolin-1 (Cav-1) to study the endocytic activity of FAE. Carbon particles entered the intercellular space of FAE, but the carbon particles were not internalized by the FAE cells...
September 15, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28914347/intermetatarsal-bursa-primary-synovial-chondromatosis-case-report-and-review-of-the-literature
#20
Manuel Trevino, Shaked Laks, Lisa Kafchinski, Dinesh K Sundarakumar, Crysela M Smith
Primary synovial chondromatosis is a benign neoplastic process, occurring mostly in large joints, more rarely in tendon sheaths, and extremely uncommonly in bursae. We describe a patient with primary synovial chondromatosis arising in the fourth intermetatarsal bursa. Knowledge of the bursal anatomy of the forefoot, and of characteristic imaging findings and the pathogenesis of synovial chondromatosis, is essential in including this uncommon entity in the differential when occurring in unusual locations.
September 15, 2017: Skeletal Radiology
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