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https://www.readbyqxmd.com/read/29333394/evaluation-of-anti-wrinkle-effects-of-duoligo-composed-of-lactulose-and-galactooligosaccharides
#1
Eun Young Jung, Jung Il Kwon, Yang Hee Hong, Hyung Joo Suh
Currently, alternatives to prebiotics for skin treatment are receiving much interest. However, little is known about the efficacy of topically applied prebiotics in skin anti-aging. This study was conducted to observe the anti-aging effects of DuOligo, which is composed of lactulose and galactooligosaccharides (GOS). We investigated wrinkle-related parameters by quantitative and qualitative skin evaluation in healthy women who consumed DuOligo for 8 weeks. The double-blind, randomized, and placebo-controlled study included subjects who were divided into two groups (Placebo: dextrin 4...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29323242/adipose-specific-lipin1-overexpression-in-mice-protects-against-alcohol-induced-liver-injury
#2
Wenliang Zhang, Wei Zhong, Qian Sun, Xinguo Sun, Zhanxiang Zhou
Excessive fatty acid release from the white adipose tissue (WAT) contributes to the development of alcoholic liver disease (ALD). Lipin1 (LPIN1), as a co-regulator of DNA-bound transcription factors and a phosphatidic acid (PA) phosphatase (PAP) enzyme that dephosphorylates PA to form diacylglycerol (DAG), is dramatically reduced by alcohol in the WAT. This study aimed at determining the role of adipose LPIN1 in alcohol-induced lipodystrophy and the development of ALD. Transgenic mice overexpressing LPIN1 in adipose tissue (LPIN1-Tg) and wild type (WT) mice were fed a Lieber-DeCarli alcohol or isocaloric maltose dextrin control liquid diet for 8 weeks...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29323180/low-amounts-of-dietary-fibre-increase-in-vitro-production-of-short-chain-fatty-acids-without-changing-human-colonic-microbiota-structure
#3
Daisuke Sasaki, Kengo Sasaki, Naoko Ikuta, Takahiro Yasuda, Itsuko Fukuda, Akihiko Kondo, Ro Osawa
This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake). We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum. Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota. This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29319093/nutriosomes-prebiotic-delivery-systems-combining-phospholipids-a-soluble-dextrin-and-curcumin-to-counteract-intestinal-oxidative-stress-and-inflammation
#4
Ana Catalán-Latorre, Maria Pleguezuelos-Villa, Ines Castangia, Maria Letizia Manca, Carla Caddeo, Amparo Nácher, Octavio Díez-Sales, José Esteban Peris, Ramon Pons, Elvira Escribano-Ferrer, Anna Maria Fadda, Maria Manconi
Nutriosomes, new phospholipid nanovesicles specifically designed for intestinal protection were developed by simultaneously loading a water-soluble dextrin (Nutriose® FM06) and a natural antioxidant (curcumin). Nutriosomes were easily fabricated in a one-step, organic solvent-free procedure. The stability and delivery performances of the vesicles were improved by adding hydroxypropyl methylcellulose. All the vesicles were small in size (mean diameter ∼168 nm), negatively charged (zeta potential ∼-38 mV, irrespective of their composition), and self-assembled predominantly in unilamellar vesicles stabilized by the presence of Nutriose®, which was located in both the inter-lamellar and inter-vesicle media, as confirmed by cryo-TEM and SAXS investigation...
January 10, 2018: Nanoscale
https://www.readbyqxmd.com/read/29290355/in%C3%A2-vitro-and-in%C3%A2-vivo-effects-of-selected-fibers-on-the-pharmacokinetics-of-orally-administered-carbamazepine-possible-interaction-between-therapeutic-drugs-and-semisolid-enteral-nutrients
#5
Katsuhito Nagai, Sachiko Omotani, Miki Otani, Moe Sasatani, Takahiro Takashima, Yasutoshi Hatsuda, Junji Mukai, Michiaki Myotoku
OBJECTIVE: The management of nutrition using semisolid enteral nutrients is considered useful for avoiding the adverse effects associated with liquid enteral nutrients. In the present study, we used an in vitro analysis to investigate whether carbamazepine (CBZ) is adsorbed by the fibers included in semisolid enteral nutrients. The effects of these fibers on the pharmacokinetic profile of CBZ following its oral administration were also examined in rats. METHODS: The adsorption of CBZ onto fibers was examined by absorbance monitoring of the filtrate after centrifugation using an ultrafiltration device...
February 2018: Nutrition
https://www.readbyqxmd.com/read/29277606/characterization-of-2-cys-peroxiredoxin-3-and-4-in-common-carp-and-the-immune-response-against-bacterial-infection
#6
Yu Zhu Yang, Yan Zhao, Ling Yang, Lan Ping Yu, Hui Wang, Xiang Shan Ji
Accumulating evidence suggests that peroxiredoxins (Prxs) eliminate excessive cellular H2O2 and are important factors in redox signaling pathways. In this study, we cloned the full-length cDNAs and genomic sequences of Prx3 and Prx4 from common carp. The common carp Prx3 and Prx4 open reading frames were 753 base pairs (bp) and 783bp in length, respectively, and contained seven exons and six introns. Multiple sequence alignment and phylogenetic analyses revealed that the common carp Prx1-4 proteins share high identities and similar characteristics with other known animal Prxs...
December 19, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29201100/single-step-purification-of-novel-thermostable-and-chelator-resistant-amylase-from-bacilluslicheniformis-rm44-by-affinity-chromatography
#7
Ayesha Siddiqui, Mustafa Kamal, Seyed Abdulmajid Ayatollahi, Mohsin Ali, Mansoor Ahmed
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened for the production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneity on a single step by affinity chromatography using insoluble corn starch. The molecular weight of Amy RM44 was estimated to be 66 kDa by SDS-PAGE and zymographic analysis. Nine fold purification was achieved with the specific activity of 870 U/mg that provides the total yield of the enzyme up to 31%. Studies on purified AmyRM44 characterization revealed that the optimum temperature of enzyme was 100 ºC...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/29151980/synbiotic-effects-of-%C3%AE-glucans-from-cauliflower-mushroom-and-lactobacillus-fermentum-on-metabolic-changes-and-gut-microbiome-in-estrogen-deficient-rats
#8
Seong-Yeop Jeong, Suna Kang, Cao Shi Hua, Zhang Ting, Sunmin Park
Background: We investigated whether the long-term consumption of a symbiotic formulation with Lactobacillus fermentum (probiotic) and β-glucan from cauliflower mushroom (prebiotic) would delay the progression of post-menopausal symptoms in ovariectomized (OVX) rats and explored their mechanisms of action, including changes in gut microbiota. Methods: OVX rats were fed with high-fat diets containing 1% dextrin (control), 1% lyophilized cauliflower mushroom extract (CFM), 0...
2017: Genes & Nutrition
https://www.readbyqxmd.com/read/29146428/preparative-fractionation-of-dextrin-by-polyethylene-glycol-effects-of-initial-dextrin-concentration-and-ph
#9
Xiuting Hu, Yu Wang, Chengmei Liu, Zhengyu Jin, Yaoqi Tian
Polyethylene glycol (PEG) was further applied for fractionating dextrin prepared from cassava starch. The initial dextrin concentration and pH of the dextrin solutions were crucially considered in this study with the average molecular-weight dispersity (DMa) as the index. The results showed that the initial dextrin concentration significantly affected the mass fraction and the molecular weight distribution of each dextrin fraction obtained from gradient PEG precipitation. However, the initial dextrin concentration, which ranged from 0...
November 11, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29134685/in-ovo-feeding-of-nutrients-and-its-impact-on-post-hatching-water-and-feed-deprivation-up-to-48%C3%A2-hr-energy-status-and-jejunal-morphology-of-chicks-using-response-surface-models
#10
M Ghanaatparast-Rashti, M Mottaghitalab, H Ahmadi
A Box-Behnken design (BBD) in a response surface methodology (RSM) was used to investigate the response of broiler chicks to in ovo feeding (IOF) of beta-hydroxy beta-methylbutyrate (HMB), dextrin and the timing of the first water and feed deprivation. On day 18th of incubation, 1,500 eggs were randomly assigned to 15 experimental runs of BBD, each with 4 replicates, as 3 levels IOF of HMB (0%, 0.5% and 1%) and dextrin (0%, 20% and 40%), and 3 levels of the first water and feed deprivation (6, 27 and 48 hr)...
November 13, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29127884/regional-dysregulation-of-taurine-and-related-amino-acids-in-the-fetal-rat-brain-following-gestational-alcohol-exposure
#11
Raine Lunde-Young, Katie Davis-Anderson, Vishal Naik, Matthew Nemec, Guoyao Wu, Jayanth Ramadoss
The fetal brain exhibits exquisite alcohol-induced regional neuronal vulnerability. A candidate mechanism for alcohol-mediated brain deficits is disruption of amino acid (AA) bioavailability. AAs are vitally important for proper neurodevelopment, as they comprise the most abundant neurotransmitters in the brain and act as neurotransmitter precursors, nitric oxide donors, antioxidants, and neurotrophic factors, which induce synaptogenesis, neuronal proliferation, and migration. We hypothesized that gestational alcohol alters brain AA concentrations, disrupts AAs associated with neuropathogenesis, and that alterations are region-specific...
September 30, 2017: Alcohol
https://www.readbyqxmd.com/read/29098410/comparative-proteomic-analysis-sclr-is-importantly-involved-in-carbohydrate-metabolism-in-aspergillus-oryzae
#12
Feng-Jie Jin, Pei Han, Miao Zhuang, Zhi-Min Zhang, Long Jin, Yasuji Koyama
The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. The result implied that SclR is potentially important in both traditional fermentative manufacturing and commercial enzyme production in A...
November 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29080707/preparation-and-characterization-of-an-amylase-triggered-dextrin-linked-graphene-oxide-anticancer-drug-nanocarrier-and-its-vascular-permeability
#13
Siaw Fui Kiew, Yan Teck Ho, Lik Voon Kiew, James Chen Yong Kah, Hong Boon Lee, Toyoko Imae, Lip Yong Chung
We synthesized a dextrin (DEX)-conjugated graphene oxide (GO) nanocarrier (GO100-DEX) as a potential drug delivery system to respond to a tumor-associated stimulus, α-amylase, that has high permeability through the fenestrated endothelial barrier to the tumor site. At acidic pH and in the presence of α-amylase to simulate tumor conditions, GO100-DEX released a 1.5-fold higher amount of doxorubicin (DOX) than of GO100. Under the same conditions, the cytotoxic effects of GO100-DEX/DOX were 2-fold greater than those of free DOX and 2...
October 27, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29078182/tungsten-vi-based-molecular-puzzle-photoluminescent-nanoparticles-easily-covered-with-biocompatible-natural-polysaccharides-via-direct-chelation
#14
Volodymyr Lobaz, Martin Hladik, Miloš Steinhart, Aleksandra Paruzel, Peter Černoch, Jiří Pánek, Miroslav Vetrík, Daniel Jirák, Markéta Jirátová, Ognen Pop-Georgievski, Miroslav Šlouf, Sébastien Garcia-Argote, Gregory Pieters, Eric Doris, Martin Hrubý
Multimodal probes, which can be simultaneously visualized by multiple imaging modalities, enable the cellular uptake, intracellular fate, biodistribution and elimination to be tracked in organisms. In this study, we report the synthesis of crystalline WO3 and CaWO4 doped with Eu(3+) or Tb(3+) nanoparticles (size range of 10-160 nm) coated with polysaccharides, and these nanoparticles constitute a versatile easy-to-construct modular toolbox for multimodal imaging. The particles adsorb significant amounts of polysaccharides from the solution, providing biocompatibility and may serve as a platform for labeling...
October 16, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29037674/structure-of-pyrodextrin-in-relation-to-its-retrogradation-properties
#15
Xiaoxian Han, Ji Kang, Yanjie Bai, Meng Xue, Yong-Cheng Shi
Pyrodextrins were prepared by heating waxy maize starch at 170°C and pH 3.0 for 0.5, 1, 2, 3 and 4h. Structural changes of pyrodextrins were determined by gel permeation chromatography and (1)H NMR. Gelatinization and retrogradation of pyrodextrins were measured by differential scanning calorimetry. The water solubility of pyrodextrins increased from 48.9 to 99.4% as heating time increased from 0.5 to 4h. The molecular size, melting enthalpy of pyrodextrin, and the degree of retrogradation decreased as pyrodextrin solubility increased...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29037665/unit-and-internal-chain-profiles-of-maca-amylopectin
#16
Ling Zhang, Guantian Li, Weirong Yao, Fan Zhu
Unit chain length distributions of amylopectin and its φ, β-limit dextrins, which reflect amylopectin internal structure from three maca starches, were determined by high-performance anion-exchange chromatography with pulsed amperometric detection after debranching, and the samples were compared with maize starch. The amylopectins exhibited average chain lengths ranging from 16.72 to 17.16, with ranges of total internal chain length, external chain length, and internal chain length of the maca amylopectins at 12...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29036399/use-of-a-crossed-high-alcohol-preferring-chap-mouse-model-with-the-niaaa-model-of-chronic-binge-ethanol-intake-to-study-liver-injury
#17
Kyle J Thompson, Shayan S Nazari, W Carl Jacobs, Nicholas J Grahame, Iain H McKillop
Aims: This study sought to compare mice bred to preferentially consume high amounts of alcohol (crossed-high alcohol preferring, cHAP) to c57BL/6 (C57) mice using a chronic-binge ethanol ingestion model to induce alcoholic liver disease (ALD). Methods: Male C57 and cHAP mice were randomized to a Lieber-DeCarli control (LDC) diet, Lieber-DeCarli 5% (v/v) ethanol (LDE) diet or free-choice between 10% (v/v) ethanol in drinking water (EtOH-DW) and DW. After 4 weeks mice were gavaged with either 9 g/kg maltose-dextrin (LDC+MD) or 5 g/kg EtOH (LDE+Binge, EtOH-DW+Binge)...
November 1, 2017: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/29031887/direct-colorimetric-detection-of-unamplified-pathogen-dna-by-dextrin-capped-gold-nanoparticles
#18
Amy M Baetsen-Young, Matthew Vasher, Leann L Matta, Phil Colgan, Evangelyn C Alocilja, Brad Day
The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection...
October 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29030291/evaluation-of-a-spray-type-novel-dextrin-hydrogel-adhesion-barrier-under-laparoscopic-conditions-in-a-porcine-uterine-horn-adhesion-model
#19
Miho Kai, Kazuha Maeda, Masako Tasaki, Shinichirou Kira, Shintarou Nakamura, Naotaka Chino, Hitomi Hagiwara, Hitoshi Nishida, Tetsuro Kawanishi
STUDY OBJECTIVE: To establish a porcine uterine horn adhesion model that mimicked laparoscopic procedures and use it to investigate the effect of a spray-type, novel dextrin hydrogel adhesion barrier (AdSpray) on postsurgical adhesions. DESIGN: Single: Single-blind randomized controlled trial (Canadian Task Force Classification I). SETTING: Certified animal research facility. SUBJECTS: 16 female pigs. INTERVENTIONS: All animals underwent laparoscopically-assisted adhesion-inducing surgery...
October 10, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29027557/ionic-surfactant-coated-subtilisin-activity-enantioselectivity-and-application-to-dynamic-kinetic-resolution-of-secondary-alcohols
#20
Kyungwoo Kim, Eungyeong Lee, Cheolwoo Kim, Jaiwook Park, Mahn-Joo Kim
In this work, we explored the utility of ionic-surfactant-coated Bacillus licheniformis subtilisin (ISCBLS) as the catalyst for the dynamic kinetic resolution of secondary alcohols. ISCBLS was prepared by freeze-drying Bacillus licheniformis subtilisin with both ionic surfactant 1 and dextrin. ISCBCL displayed 9300-fold enhanced activity relative to its native counterpart in the transesterificaion of N-acetyl phenylalanine ethyl ester with 1-propanol in hexane and 12 800-fold enhanced activity in the transesterification of trifluoroethyl butyrate with 1-phenylethanol in THF...
October 25, 2017: Organic & Biomolecular Chemistry
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