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Víctor-Guillermo Sequera, Marisa Mena, Malen Hollmann, Estefani Mani, Viviana Ramas, Sylvia Bonilla, Alicia Guerra, Fernando Borgia
OBJECTIVES: To identify factors associated with high-risk human papillomavirus (HPV) infection and high grade squamous intraepithelial lesion (HSIL) among a high-risk group of HPV-unvaccinated women in Montevideo. METHODS: Participants completed a questionnaire on socio-demographics, sexual behavior and gynecological history and received a gynecological examination. HPV DNA was detected by PCR using MY09/11 primers. Logistic regression analyses were performed to identify factors associated with high-risk HPV infection and HSIL...
March 16, 2018: Papillomavirus Research
F Hinten, A Molijn, L Eckhardt, L F A G Massuger, W Quint, P Bult, J Bulten, W J G Melchers, J A de Hullu
BACKGROUND: Two etiologic pathways for vulvar squamous cell carcinoma (SCC) are described: in a background of lichen sclerosus and/or differentiated vulvar intraepithelial neoplasia and related to high-risk human papillomavirus (HPV) infection with high grade squamous intraepithelial lesion (HSIL) as precursor. The aim was to compare the predilection site and survival of HPV-related to non HPV-related vulvar SCCs. METHODS: Data of patients treated for primary vulvar SCC at the Radboudumc between March 1988 and January 2015 were analyzed...
March 16, 2018: Gynecologic Oncology
Geilson Gomes de Oliveira, Judite Maria da Silva Costa de Oliveira, Renata Mirian Nunes Eleutério, José Eleutério Júnior
OBJECTIVE:  To assess the management chosen by gynecologists after atypical squamous cells (ASCs) cytology results, and to evaluate the outcomes of these cases in Brazilian women. METHODS:  A prospective observational study evaluated the initial management offered by the gynecologist in the case of 2,458 ASCs cytology results collected between January of 2010 and July of 2016. The outcomes of the cytology, high-risk human papilloma virus (HR-HPV) test and histology were compared in two subgroups: atypical squamous cells of undetermined significance (ASC-US) and atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion (ASC-H)...
March 19, 2018: Revista Brasileira de Ginecologia e Obstetrícia
Yimin Ge, Paul Christensen, Eric Luna, Donna Armylagos, Jiaqiong Xu, Mary R Schwartz, Dina R Mody
OBJECTIVE: Human papillomavirus (HPV) tests and genotyping (GT) have been used in clinical risk assessment. The purpose of this study was to analyze the performance of 2 common HPV testing platforms in risk evaluation for high-grade cervical lesions. MATERIALS AND METHODS: Between January 1, 2015, and December 31, 2016, a total of 4,562 Pap tests with follow-up biopsies in our laboratory database were analyzed along with HPV tests performed on Cobas (CHPV, n = 3,959) or Aptima (AHPV, n = 603) platforms...
March 14, 2018: Journal of Lower Genital Tract Disease
Ling Xu, Jun Xu, Zheng Hu, Baohua Yang, Lifeng Wang, Xiao Lin, Ziyin Xia, Zhiling Zhang, Yunheng Zhu
DNA methylation is associated with tumorigenesis and may act as a potential biomarker for detecting cervical cancer. The aim of the present study was to explore the methylation status of the paired box gene 1 (PAX1) and the LIM homeobox transcription factor 1 α (LMX1A) gene in a spectrum of cervical lesions in an Eastern Chinese population. This single-center study involved 121 patients who were divided into normal cervix (NC; n=28), low-grade squamous intraepithelial lesion (LSIL; n=32), high-grade squamous intraepithelial lesion (HSIL; n=34) and cervical squamous cell carcinoma (CSCC; n=27) groups, according to biopsy results...
April 2018: Oncology Letters
Ross D Cranston, Michelle S Cespedes, Pawel Paczuski, Ming Yang, Robert W Coombs, Joan Dragavon, Alfred Saah, Catherine Godfrey, Jennifer Y Webster-Cyriaque, Elizabeth Y Chiao, Barbara Bastow, Timothy Wilkin
BACKGROUND: The quadrivalent human papillomavirus (HPV) vaccine (qHPV; types 6, 11, 16, 18) is indicated for men and women aged 9 to 26 years to prevent HPV associated anogenital high-grade squamous intraepithelial lesions (HSIL) and cancer. ACTG 5298 was a randomized placebo controlled Phase 3 study in human immunodeficiency virus (HIV)-infected men who have sex with men, and women of qHPV to prevent persistent anal HPV infection. Baseline data are presented here. METHODS: Human immunodeficiency virus-infected men who have sex with men, and women 27 years or older without previous anogenital or oral cancer were enrolled...
April 2018: Sexually Transmitted Diseases
Fatma Beyazit, Fatma Sılan, Meryem Gencer, Buket Aydin, Barış Paksoy, Mesut A Unsal, Ozturk Ozdemir
OBJECTIVES: Cervical cancer is the second most common type of cancer for women worldwide with a great proportion proved to be related to human papillomavirus (HPV) infection. As infection with HPV is the strongest risk factor for cervical neoplasia, detection of HPV genotypes in cervical and vaginal specimens of women with normal and abnormal cytology seems to be of paramount importance in cervical cancer screening. The objective of the study is to evaluate the prevalence and HPV genotypes among women with normal or abnormal Pap smear tests...
2018: Ginekologia Polska
Kiyong Na, Ji-Youn Sung, Hyun-Soo Kim
BACKGROUND/AIM: Severe nuclear atypia can be associated with condyloma acuminatum. In this study, we investigated nine cases of perianal condyloma acuminatum with severe nuclear atypia and determined whether severe nuclear atypia is sufficient for the diagnosis of high-grade squamous intraepithelial lesion (HSIL). MATERIALS AND METHODS: The clinical data and pathological features of the nine patients were collected. p16 Immunostaining and human papillomavirus genotyping were also performed...
March 2018: Anticancer Research
Jaron Mark, Kayla Morrell, Kevin Eng, Alexandra Alfiero, Peter J Frederick
OBJECTIVES: The aim of the study was to evaluate whether expert review of outside cervical cytology affects patient care. MATERIALS AND METHODS: A retrospective study was conducted of 424 new patient referrals for cervical dysplasia between 2004 and 2016 at Roswell Park Cancer Institute. Records were analyzed for outside cervical cytology reports and compared with expert cervical cytology review. Differences between expert review and outside reports were documented...
February 23, 2018: Journal of Lower Genital Tract Disease
Ross D Cranston, Michelle S Cespedes, Pawel Paczuski, Ming Yang, Robert W Coombs, Joan Dragavon, Alfred Saah, Catherine Godfrey, Jennifer Y Webster-Cyriaque, Elizabeth Y Chiao, Barbara Bastow, Timothy Wilkin
BACKGROUND: The quadrivalent human papillomavirus (HPV) vaccine (qHPV; types 6, 11, 16, 18) is indicated for men and women 9-26 years to prevent HPV associated anogenital high grade squamous intraepithelial lesions (HSIL) and cancer. ACTG 5298 was a randomized placebo controlled Phase 3 study in HIV-infected men who have sex with men (MSM), and women of qHPV to prevent persistent anal HPV infection. Baseline data is presented here. METHODS: HIV-infected MSM, and women ≥ 27 years without previous anogenital or oral cancer were enrolled...
October 13, 2017: Sexually Transmitted Diseases
Monique Figueiredo Teixeira, Meritxell Sabidó, André Luiz Leturiondo, Cynthia de Oliveira Ferreira, Kátia Luz Torres, Adele Schwartz Benzaken
BACKGROUND: Human immunodeficiency virus (HIV)-positive women have a high prevalence of human papillomavirus (HPV), and are infected with a broader range of HPV types than HIV-negative women. We aimed to determine the prevalence of cervical cytologic abnormalities, high-risk (HR)-HPV prevalence, type distribution according to the severity of cervical lesions and CD4 cell count and identify factors associated with HR-HPV infection among women living with HIV in Manaus, Amazonas. METHODS: We enrolled 325 women living with HIV that attended an infectious diseases referral hospital...
February 17, 2018: Virology Journal
Viviana Ramas, Santiago Mirazo, Sylvia Bonilla, Dora Ruchansky, Juan Arbiza
OBJECTIVE: This study aims to investigate the HPV16 variant distribution by sequence analyses of E6, E7 oncogenes and the Long Control Region (LCR), from cervical cells collected from Uruguayan women, and to reconstruct the phylogenetic relationships among variants. METHODS: Forty-seven HPV16 variants, obtained from women with HSIL, LSIL, ASCUS and NILM cytological classes were analyzed for LCR and 12 were further studied for E6 and E7. Detailed sequence comparison, genetic heterogeneity analyses and phylogenetic reconstruction were performed...
February 14, 2018: Gene
Kunihisa Uchita, Kenji Kanenishi, Koki Hirano, Hideki Kobara, Noriko Nishiyama, Ai Kawada, Shintaro Fujihara, Emi Ibuki, Reiji Haba, Yohei Takahashi, Yuka Kai, Kenji Yorita, Hirohito Mori, Jun Kunikata, Naoki Nishimoto, Toshiyuki Hata, Tsutomu Masaki
BACKGROUND: Colposcopy, which is a standard modality for diagnosing cervical intraepithelial neoplasia (CIN), can have limited accuracy owing to poor visibility. Flexible magnifying endoscopy with narrow band imaging (ME-NBI) has excellent diagnostic accuracy for early gastrointestinal neoplasms and is expected to be highly useful for CIN diagnosis. This study aimed to determine the characteristic findings and evaluate the diagnostic ability of ME-NBI for lesions ≥ CIN 3. METHODS: A well-designed prospective diagnostic case series conducted at multiple tertiary-care centers...
February 14, 2018: International Journal of Clinical Oncology
Andreia Albuquerque, Elisabete Rios, Claudia Camila Dias, Mayura Nathan
p16 is the most widely studied biomarker in lower anogenital tract squamous intraepithelial lesions and, currently the only recommended biomarker for histological grade assessment. The aim of this systematic review and meta-analysis was to evaluate p16-positive rates according to anal squamous intraepithelial lesions/anal intraepithelial neoplasia (AIN) grade. Two investigators independently searched four electronic databases: PubMed, Web of Sciences, Scopus, and Embase from inception until August 2017. Studies that evaluated p16 immunostaining in histological samples of anal and/or perianal squamous intraepithelial lesions and defined a p16-positive result as diffuse block staining with nuclear or nuclear plus cytoplasmic staining were included...
February 13, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Katrin Christine Asciutto, Avalon Ernstson, Ola Forslund, Christer Borgfeldt
BACKGROUND: In order to increase coverage in the organized cervical screening program, self-sampling with HPV analyses has been suggested. OBJECTIVES: The aim was to compare human papillomavirus (HPV) mRNA detection in vaginal and urine self-collected samples with clinician-taken cervical samples and the corresponding clinician-taken histological specimens. STUDY DESIGN: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 209 women with the Aptima mRNA assay (Hologic Inc, MA, USA)...
February 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Irena Aleksioska-Papestiev, Vesna Chibisheva, Megi Micevska, Goran Dimitrov
Introduction: Cervical cancer is a malignancy originating in the transformation zone of the cervix, most commonly in the squamous cells. It is the fourth most common cancer in women worldwide, and the third most common cause of female cancer death. Genital human papilloma viruses (HPV) are sexually transmitted and approximately 630 milion people worldwide are infected. More than 200 genotypes, subtypes and variants have been reported, 13-15 being oncogenic type, which could be responsible for cervical intraepithelial lesions (CIN) or cancer...
February 2018: Medical Archives
Lu Liu, Chunmei Ying, Zhen Zhao, Long Sui, Xinyan Zhang, Chunyan Qian, Qing Wang, Limei Chen, Qisang Guo, Jiangnan Wu
Background: The dynamic methylation of human papillomavirus (HPV) 16 DNA is thought to be associated with the progression of cervical lesions. Previous studies that did not consider the physical status of HPV 16 may have incorrectly mapped HPV 16 methylomes. In order to identify reliable biomarkers for squamous cervical cancer (SCC), we comprehensively evaluated the methylation of HPV 16 depending on the integration incidence of each sample. Methods: Based on the integration status of 115 HPV 16-infected patients (50 SCC, 30 high-grade squamous intraepithelial lesion [HSIL], and 35 low-grade squamous intraepithelial lesion [LSIL]) and HPV 16-infected Caski cell lines by PCR detection of integrated papillomavirus sequences, we designed a series of primers that would not be influenced by breakpoints for a high-resolution melting (HRM) PCR method to detect the genome methylation...
2018: Clinical Epigenetics
Ahmed M Maged, Hany Saad, Emad Salah, Hadeer Meshaal, Mostafa AbdElbar, Eman Omran, Ashraf Eldaly
OBJECTIVE: To assess the sensitivity of a urine test for high-risk HPV DNA genotypes in the detection of high-grade squamous intra-epithelial lesion (HSIL) and its correlation with pathologic precancerous lesions. METHODS: The present prospective cross-sectional study included women referred to Kasr AlAiny Medical School, Cairo, Egypt, for cervical smear anomalies, a history of cervical smear anomalies, or for suspicious cervix between May 1, 2015, and April 30, 2017...
January 31, 2018: International Journal of Gynaecology and Obstetrics
Shu-Hsing Cheng, Kuo-Sheng Liao, Chi-Chao Wang, Chien-Yu Cheng, Fang-Yeh Chu
OBJECTIVES: This study aimed to assess the relationship between infection with multiple human papillomavirus (HPV) types and abnormal anal cytology in HIV-infected men. DESIGN: An observational, cross-sectional study. SETTING: A regional referral hospital in Taiwan. PARTICIPANTS: In total, 714 HIV-infected men were enrolled between March 2011 and June 2016. Thin preparation anal Pap smears were interpreted according to the 2001 Bethesda System...
January 27, 2018: BMJ Open
V N Saritha, V S Veena, K M Jagathnath Krishna, Thara Somanathan, K Sujathan
Cervical cancer continues to be a leading cancer among women in many parts of the world. Nation-wide screening with the Pap smear has not been implemented in India due to the lack of adequately trained cytologists. Identification of biomarkers to predict malignant potential of the identified low risk lesions is essential to avoid excessive retesting and follow up. The current study analyzed the expression patterns of DNA replication licensing proteins, proliferation inhibitor protein p16INK4A and tumor suppresser protein p63 in cervical tissues and smears to assess the ability of these proteins to predict progression...
January 27, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
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