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https://www.readbyqxmd.com/read/29314149/uniplex-e6-e7-pcr-method-detecting-e6-or-e7-genes-in-39-human-papillomavirus-types
#1
Mitsuaki Okodo, Kaori Okayama, Koji Teruya, Toshiyuki Sasagawa
We have developed a new human papillomavirus (HPV) assay using the uniplex E6/E7 polymerase chain reaction (PCR) method, which is able to detect E6 and E7 genes in 39 HPV types. We validated the assay for sensitivity and specificity using cloned HPV DNA and clinical samples. A comparative study using Genosearch-31 (GS-31) to determine HPV genotypes in clinical samples was also performed. E6 or E7 genes, measured by uniplex E6/E7 PCR, were detectable in 15 low-risk (HPV-6, -11, -40, -42, -44, -54, -55, -61, -62, -71, -74, -81, -84, -89, -90), 11 intermediate-risk (HPV-26, -30, -34, -53, -66, -67, -69, -70, -73, -82, -85), and 13 high-risk (HR) HPV types...
January 4, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29286606/human-papilloma-virus-genotype-distribution-in-cervical-lesions-in-zanjan-iran
#2
Shahrzad Ahmadi, Hossein Goudarzi, Ahmad Jalilvand, Abdolreza Esmaeilzadeh
Objective: Cervical cancer is one of the most common cancers among women all over the world, and main cause is persistent infection with high risk human papillomavirus (HPV) strains. It has been reported that the distribution and prevalence of HPV types varies by geographical region, so that this is important for prevention by type-specific vaccines. The aim of current study was to determine the genotype distribution of HPV using the INNO-LiPA genotyping assay in Zanjan province, North West Iran. Methods: A total of 112 formalin-fixed paraffin embedded (FFPE) tissue samples from cases of low-grade intraepithelial lesion (LSIL), high-grade intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC) were collected...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29247086/risk-of-high-grade-lesions-after-atypical-glandular-cells-in-cervical-screening-a-population-based-cohort-study
#3
Ingrid Norman, Anders Hjerpe, Joakim Dillner
OBJECTIVES: To determine how human papillomavirus (HPV) positivity of atypical glandular cells (AGCs) affects the predictive values for the presence of high-grade cervical lesions. DESIGN: Population-based cohort study. SETTING: Stockholm-Gotland region, Sweden. PARTICIPANTS: Between 17 February 2014 and 30 June 2016, there were 562 women with AGC detected in a cervical sample. Registry linkages up to 30 June 2016 identified 392 women with an associated HPV test and a histopathological follow-up...
December 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/29236173/is-surgical-plume-developing-during-routine-leeps-contaminated-with-high-risk-hpv-a-pilot-series-of-experiments
#4
Kay Neumann, Markus Cavalar, Achim Rody, Luisa Friemert, Daniel A Beyer
INTRODUCTION: Growing evidence shows a causal role of high-risk humane papillomavirus (HPV) infections in the development of head and neck cancer. A recent case report shows two patients suffering from tonsillar cancer without any risk factors apart from their work as gynecologists doing laser ablations and loop electrosurgical excision procedures (LEEP). The aim of the present investigation is to evaluate whether surgical plume resulting from routine LEEPs of HSIL of the cervix uteri might be contaminated with the DNA of high-risk HPV...
December 13, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29230975/negative-pap-tests-in-women-with-high-grade-cervical-lesions-on-follow-up-biopsies-contributing-factors-and-role-of-human-papillomavirus-genotyping
#5
Steven Goodman, Roxanne R Mody, Donna Coffey, Blythe K Gorman, Eric Luna, Donna Armylagos, Mary R Schwartz, Dina R Mody, Yimin Ge
BACKGROUND: Previous studies have indicated that negative Papanicolaou (Pap) tests can precede high-grade cervical lesions (HGCL) on biopsy. This study aims to determine the contributing factors for cytologic discrepancy and the potential role of human papilloma virus (HPV) testing in risk evaluation of women with negative Pap tests. METHODS: Of 42,797 Pap tests interpreted as negative for intraepithelial lesion or malignancy (NILM) from March 1, 2013 to December 30, 2014, 426 had available HPV testing and follow-up biopsy...
December 12, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29223946/-quality-of-the-exo-endocervical-sampling-in-the-prevention-of-uterine-cervix-cancer
#6
Anabela A Angeleri, Lili B Díaz, Gisela Coliva, Fernando Guerra, Luis A Palaoro, Adriana E Rocher
Cervical and vaginal cytology, Papanicolaou test (PAP), is the most effective test for screening of preneoplastic lesions, and cervical cancer prevention. Its sensitivity goes from 50 to 98%, according to different statistics, and this variation is related to the sampling procedure. A satisfactory smear should be taken from the transformation zone, the junction between endocervix and exocervix. According to Bethesda, metaplastic and/or endocervical cells should be observed under the microscope. The traditional PAP smear includes an exo-endocervical sampling using the Ayre spatula; however, only near 50% of the smears are representative of the transformation zone...
2017: Medicina
https://www.readbyqxmd.com/read/29215211/a-study-of-hormonal-effects-in-cervical-smear-samples-using-raman-spectroscopy
#7
Damien Traynor, Padraig Kearney, Ines Ramos, Cara M Martin, John J O'Leary, Fiona M Lyng
Raman spectroscopy is a powerful tool that has the potential to be used as a screening method for cervical cancer. It is a label free, low cost method providing a biochemical fingerprint of a given sample. The objective of this study was to address patient to patient variability contributed by hormonal effects due to the menstrual cycle, the use of hormone based contraceptives and the onset of menopause, and to determine if these changes would affect the ability to successfully identify dyskaryotic cells. Raman spectra were recorded from unstained ThinPrep cervical samples (45 cytology negative and 15 high grade dyskaryosis (high grade squamous intraepithelial lesion (SIL)) samples using a HORIBA Jobin Yvon XploRA™ system...
December 7, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29210158/screening-for-human-papillomavirus-cervical-cytological-abnormalities-and-associated-risk-factors-in-hiv-positive-and-hiv-negative-women-in-rwanda
#8
M F Mukanyangezi, V Sengpiel, O Manzi, G Tobin, S Rulisa, E Bienvenu, D Giglio
OBJECTIVES: Cervical cancer is the major cause of death from cancer in Africa. We wanted to assess the prevalence of human papillomavirus (HPV) infections and associated risk factors and to determine whether HPV testing could serve as a screening method for squamous intraepithelial lesions (SILs) in Rwanda. We also wanted to obtain a broader understanding of the underlying risk factors for the establishment of HPV infection in Rwanda. METHODS: A total of 206 HIV-positive women, 172 HIV-negative women and 22 women with unknown HIV status were recruited at the University Teaching Hospitals of Kigali (UTHK) and of Butare (UTHB) in Rwanda...
December 6, 2017: HIV Medicine
https://www.readbyqxmd.com/read/29208455/high-prevalence-and-incidence-of-hpv-related-anal-cancer-precursor-lesions-in-hiv-positive-women-in-the-late-haart-era
#9
Carmen Hidalgo-Tenorio, Samantha E de Jesus, Javier Esquivias, Juan Pasquau
INTRODUCTION: Anal cancer is one of the most common non-AIDS defining malignancies, especially in men who have sex with men and women living with HIV (WLHIV). OBJECTIVES: To evaluate the prevalence and incidence of precursor lesions (high-grade squamous intraepithelial lesions [HSIL]) and anal cancer in our cohort of women and to compare them to cervical lesions; to calculate the percentage of patients that acquire and clear oncogenic genotypes (HR-HPV) in the anal canal; and to determine predictive factors for anal HPV infection...
December 2, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29207280/hybrid-capture-as-a-tool-for-cervical-lesions-screening-in-hiv-infected-women-insights-from-a-brazilian-cohort
#10
Emilia Moreira Jalil, Paula M Luz, Marcel Quintana, Ruth Khalili Friedman, Rosa M Domingues S Madeira, Angela Cristina Andrade, Janice Chicarino, Ronaldo Ismerio Moreira, Monica Derrico, José Eduardo Levi, Fabio Russomano, Valdilea Gonçalves Veloso, Beatriz Grinsztejn
INTRODUCTION: Cervical cancer remains an important burden for HIV-infected women in the era of combination antiretroviral therapy. Recommendations for cervical screening in these women diverge and may include high-risk HPV (HRHPV) testing. We aimed to evaluate the clinical usefulness of a single HRHPV testing for cervical screening of HIV-infected women. METHODS: 723 HIV-infected women from a Brazilian prospective cohort were included between 1996 and 2012. Inclusion criteria were: normal cervical cytology at baseline and having a HRHPV-test at baseline...
December 2, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29205018/update-on-current-care-guideline-cytological-changes-in-the-cervix-vagina-and-vulva
#11
(no author information available yet)
Annually approximately 160 new cervical cancers are diagnosed in Finland. Screening has decreased both incidence and mortality by 80%. Both primary HPV-testing and Pap smear can be used in screening. In the future HPV vaccination will decrease the number of cervical cancers. Abnormal findings in Pap smears indicate management. LSIL lesions are followed up especially among young women and HSIL lesions treated. Follow-up after treatment should be reliably arranged, because increased risk of cancer remains ever after treatment...
2017: Duodecim; Lääketieteellinen Aikakauskirja
https://www.readbyqxmd.com/read/29204065/anal-cancer-and-anal-cancer-precursors-in-women-with-a-history-of-hpv-related-dysplasia-and-cancer
#12
Elizabeth A Stier, Elizabeth Y Chiao
The epidemiology of anal cancer in the U.S. has changed over the past 3 decades. During this period the incidence of anal cancer has increased among both men and women. Of note, women with a history of anogenital HPV infection are at higher risk than the general population for anal cancer. The increased risk ranged from increased incidence rate ratios ranging from 1.82 to 6.3 in women with a history of cervical cancer, to 4.2-16.4 in women with a history of prior cervical intraepithelial neoplasia 3 (CIN 3)...
June 2017: Seminars in Colon & Rectal Surgery
https://www.readbyqxmd.com/read/29202724/epcam-expression-in-squamous-cell-carcinoma-of-the-uterine-cervix-detected-by-monoclonal-antibody-to-the-membrane-proximal-part-of-epcam
#13
Warangkana Chantima, Charin Thepthai, Pornsuk Cheunsuchon, Tararaj Dharakul
BACKGROUND: Epithelial cell adhesion molecule (EpCAM) is a promising biomarker for squamous cell carcinoma (SCC) of the uterine cervix, because it is over-expressed in various cancers of epithelial origin. However, EpCAM expression reported in previous immunohistochemistry (IHC) studies was inconsistent. We hypothesize that the membrane-distal part of EpCAM may be lost during tissue preparation, leaving only the membrane-proximal part of EpCAM available for antibody binding and IHC staining...
December 4, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29190817/screening-for-precancerous-anal-lesions-with-p16-ki67-immunostaining-in-hiv-infected-msm
#14
Sergio Serrano-Villar, Beatriz Hernández-Novoa, Amparo de Benito, Jorge Del Romero, Antonio Ocampo, José Ramón Blanco, Mar Masiá, Elena Sendagorta, Gonzalo Sanz, Santiago Moreno, José A Pérez-Molina
BACKGROUND: Screening of anal cancer in HIV-infected MSM with anal cytology results in high rates of false positive results and elevated burden of high-resolution anoscopies. High-risk HPV up-regulates p16 and Ki67 expression in epithelial cells. We assessed the usefulness of P16/Ki-67 immunostaining cytology for the diagnosis of precancerous anal lesions. METHODOLOGY: Cross-sectional multicenter study. Concomitant anal liquid cytology with p16/Ki-67 immunostaining and HRA with biopsy of acetowhite lugol-negative lesions was performed in HIV-infected MSM...
2017: PloS One
https://www.readbyqxmd.com/read/29185272/prevalence-of-human-papillomavirus-genotypes-and-precancerous-cervical-lesions-in-a-screening-population-in-the-republic-of-korea-2014-2016
#15
Yung Taek Ouh, Kyung Jin Min, Hyun Woong Cho, Moran Ki, Jin Kyoung Oh, Sang Yop Shin, Jin Hwa Hong, Jae Kwan Lee
OBJECTIVE: Knowledge regarding the prevalence and distribution of human papillomavirus (HPV) genotyping in healthy women is important in establishing strategies for cervical cancer screening and HPV vaccination. METHODS: A total of 18,170 women who visited a Korean Medical Institute for health check-ups were recruited retrospectively; they underwent HPV genotyping and conventional cervical cytology. An HPV DNA test was performed using the Anyplex™ II HPV 28 detection system (Seegene) or HPV Liquid Bead Microarray (Osang Healthcare)...
January 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29185266/are-vaginal-swabs-comparable-to-cervical-smears-for-human-papillomavirus-dna-testing
#16
Liselotte Coorevits, Ans Traen, Luc Bingé, Jo Van Dorpe, Marleen Praet, Jerina Boelens, Elizaveta Padalko
OBJECTIVE: Human papillomavirus (HPV) testing is widely incorporated into cervical cancer screening strategies. Current screening requires pelvic examination for cervical sampling, which may compromise participation. The acceptance could be raised by introducing testing on vaginal swabs. We explored the interchangeability of vaginal swabs and cervical smears for HPV testing, by means of a prospective study conducted in female sex workers (FSWs). Besides, we report on the occurrence of 32 different HPV genotypes in FSW with low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL)...
January 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29183133/distribution-of-human-papillomavirus-genotype-in-anal-condyloma-acuminatum-among-japanese-men-the-higher-prevalence-of-high-risk-human-papillomavirus-in-men-who-have-sex-with-men-with-hiv-infection
#17
Satomi Furukawa, Shin Uota, Tetsuo Yamana, Rikisaburo Sahara, Kuniko Iihara, Yoshiyuki Yokomaku, Yasumasa Iwatani, Wataru Sugiura
Human papillomavirus (HPV) infection is known to cause anal condyloma acuminatum (CA) and squamous cell carcinoma. Men who have sex with men (MSM) with human immunodeficiency virus (HIV) infection are frequently co-infected with HPV, especially high risk HPV (HR-HPV) that causes anal squamous cell carcinoma. However, there are few reports of HPV genotype studies in anal lesion of Japanese men. We tried to estimate the distribution of HPV genotypes in anal CA tissue specimens from the Japanese men in order to elucidate the risk of anal cancer...
November 28, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29183021/digital-applications-in-cytopathology-problems-rationalizations-and-alternative-approaches
#18
Nikolaos Chantziantoniou, Maheswari Mukherjee, Amber D Donnelly, Liron Pantanowitz, R Marshall Austin
OBJECTIVE: The aim of this work was to raise awareness of problems using digital applications for examining, teaching, and applying telecytology at King Abdulaziz Medical City (KAMC), Riyadh, Saudi Arabia; University of Nebraska Medical Center (UNMC), Omaha, NE, USA; and University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, USA. The objective was to rationalize problems and propose alternative digital approaches. STUDY DESIGN: We sought to identify solutions to improve the following: (a) interpretive examination scores at KAMC for complex cytological templates (i...
November 29, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29182627/human-papillomavirus-dna-detection-in-plasma-and-cervical-samples-of-women-with-a-recent-history-of-low-grade-or-precancerous-cervical-dysplasia
#19
Clementina Elvezia Cocuzza, Marianna Martinelli, Federica Sina, Andrea Piana, Giovanni Sotgiu, Tiziana Dell'Anna, Rosario Musumeci
Circulating HPV DNA has been previously described in women with advanced stages of cervical cancer and has been suggested to be a prognostic marker of disease recurrences and metastases. Only a few studies have reported the presence of HPV DNA in bloodstream of patients with low grade or precancerous cervical lesions. This study aimed to define if HPV DNA could be detected in plasma samples of 120 women referred for a recent history of cervical dysplasia who presented with lesions ranging from High Squamous Intraepithelial Lesion (H-SIL) to regressed normal cytology...
2017: PloS One
https://www.readbyqxmd.com/read/29179876/syndemic-synergy-of-hpv-and-other-sexually-transmitted-pathogens-in-the-development-of-high-grade-anal-squamous-intraepithelial-lesions
#20
Jenny C McCloskey, W Martin Kast, James P Flexman, Dugald McCallum, Martyn A French, Michael Phillips
BACKGROUND: Anal intraepithelial neoplasia is associated with high-risk human papillomavirus (hrHPV) as a precursor to anal cancer. However, factors other than hrHPV are likely to be involved and further study of cofactors is required because of the possibility of syndemic interactions. METHODS: Three hundred and fourteen patients underwent 457 operations. Histopathology and hrHPV testing using the Digene Hybrid Capture 2 (HC 2) method were performed. Demographic factors and sexually transmissible infections (STIs) were recorded...
December 2017: Papillomavirus Research
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