keyword
MENU ▼
Read by QxMD icon Read
search

Enteroid

keyword
https://www.readbyqxmd.com/read/29467318/adenovirus-infection-of-human-enteroids-reveals-interferon-sensitivity-and-preferential-infection-of-goblet-cells
#1
Mayumi K Holly, Jason G Smith
Human adenoviruses (HAdV) are significant human pathogens. Although only a subset of HAdV serotypes commonly cause gastroenteritis in humans, most HAdV species replicate in the gastrointestinal tract. Knowledge of the complex interaction between HAdVs and the human intestinal epithelium has been limited by the lack of a suitable cell culture system containing relevant cell types. Recently, this need has been met by the stable and prolonged cultivation of primary intestinal epithelial cells as enteroids. Human enteroids have been used to reveal novel and interesting aspects of rotavirus, norovirus, and enterovirus replication, prompting us to explore their suitability for HAdV culture...
February 21, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29464078/effects-of-a-small-molecule-r-spondin-1-substitute-rs-246204-on-a-mouse-intestinal-organoid-culture
#2
Myeong-Ok Nam, Soojung Hahn, Joo Hyun Jee, Tae-Sun Hwang, Ho Yoon, Dong Hyeon Lee, Min-Soo Kwon, Jongman Yoo
Organoids, a multi-cellular and organ-like structure cultured in vitro , can be used in a variety of fields such as disease modeling, drug discovery, or cell therapy development. When organoids derived from Lgr5 stem cells are cultured ex vivo , recombinant R-spondin-1 protein should be added at a high concentration for the initiation and maintenance of the organoids. Because the addition of large amounts of R-spondin-1 greatly increases the cost of organoids, the organoids grown with R-spondin-1 are not practical for large-scale drug screening and for the development of therapeutic agents...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29463660/novel-segment-and-host-specific-patterns-of-enteroaggregative-escherichia-coli-adherence-to-human-intestinal-enteroids
#3
Anubama Rajan, Lucy Vela, Xi-Lei Zeng, Xiaomin Yu, Noah Shroyer, Sarah E Blutt, Nina M Poole, Lily G Carlin, James P Nataro, Mary K Estes, Pablo C Okhuysen, Anthony W Maresso
Enteroaggregative Escherichia coli (EAEC) is an important diarrheal pathogen and a cause of both acute and chronic diarrhea. It is a common cause of pediatric bacterial diarrhea in developing countries. Despite its discovery in 1987, the intestinal tropism of the pathogen remains unknown. Cell lines used to study EAEC adherence include the HEp-2, T-84, and Caco-2 lines, but they exhibit abnormal metabolism and large variations in gene expression. Animal models either do not faithfully manifest human clinical symptoms or are cumbersome and expensive...
February 20, 2018: MBio
https://www.readbyqxmd.com/read/29438433/attempts-to-grow-human-noroviruses-a-sapovirus-and-a-bovine-norovirus-in-vitro
#4
Tomoichiro Oka, Garrett T Stoltzfus, Chelsea Zhu, Kwonil Jung, Qiuhong Wang, Linda J Saif
Noroviruses (NoVs) and Sapoviruses (SaVs) are enteric caliciviruses that have been detected in multiple mammalian species, including humans. Currently, efficient cell culture systems have been established only for murine NoVs and porcine SaV Cowden strain. Establishment of an efficient in vitro cell culture system for other NoVs and SaVs remains challenging; however, human NoV (HuNoV) replication in 3D cultured Caco-2 cells and a clone of Caco-2 cells, C2BBe1, human enteroids and in human B cells has been reported...
2018: PloS One
https://www.readbyqxmd.com/read/29433868/critical-intestinal-cells-originate-from-the-host-in-enteroid-derived-tissue-engineered-intestine
#5
Barrett P Cromeens, Yijie Wang, Yanchun Liu, Jed Johnson, Gail E Besner
BACKGROUND: Enteroid-derived tissue-engineered intestine (TEI) contains intestinal subepithelial myofibroblasts (ISEMFs) and smooth muscle cells (SMCs). However, these cell types are not present in the donor enteroids. We sought to determine the origin of these cell types and to quantify their importance in TEI development. MATERIALS AND METHODS: Crypts from pan-EGFP or LGR5-EGFP mice were used for enteroid culture and subsequent implantation for the production of TEI...
March 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29411774/gpr43-mediates-microbiota-metabolite-scfa-regulation-of-antimicrobial-peptide-expression-in-intestinal-epithelial-cells-via-activation-of-mtor-and-stat3
#6
Ye Zhao, Feidi Chen, Wei Wu, Mingming Sun, Anthony J Bilotta, Suxia Yao, Yi Xiao, Xiangsheng Huang, Tonyia D Eaves-Pyles, George Golovko, Yuriy Fofanov, Warren D'Souza, Qihong Zhao, Zhanju Liu, Yingzi Cong
The antimicrobial peptides (AMP) produced by intestinal epithelial cells (IEC) play crucial roles in the regulation of intestinal homeostasis by controlling microbiota. Gut microbiota has been shown to promote IEC expression of RegIIIγ and certain defensins. However, the mechanisms involved are still not completely understood. In this report, we found that IEC expression levels of RegIIIγ and β-defensins 1, 3, and 4 were lower in G protein-coupled receptor (GPR)43-/- mice compared to that of wild-type (WT) mice...
February 7, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/29276155/jak-stat-1-signaling-is-required-for-reserve-intestinal-stem-cell-activation-during-intestinal-regeneration-following-acute-inflammation
#7
Camilla A Richmond, Hannah Rickner, Manasvi S Shah, Tracy Ediger, Luke Deary, Fanny Zhou, Alessio Tovaglieri, Diana L Carlone, David T Breault
The intestinal epithelium serves as an essential barrier to the outside world and is maintained by functionally distinct populations of rapidly cycling intestinal stem cells (CBC ISCs) and slowly cycling, reserve ISCs (r-ISCs). Because disruptions in the epithelial barrier can result from pathological activation of the immune system, we sought to investigate the impact of inflammation on ISC behavior during the regenerative response. In a murine model of αCD3 antibody-induced small-intestinal inflammation, r-ISCs proved highly resistant to injury, while CBC ISCs underwent apoptosis...
December 18, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29274870/villin-1-and-gelsolin-regulate-changes-in-actin-dynamics-that-affect-cell-survival-signaling-pathways-and-intestinal-inflammation
#8
Swati Roy, Amin Esmaeilniakooshkghazi, Srinivas Patnaik, Yaohong Wang, Sudeep P George, Afzal Ahrorov, Jason K Hou, Allan J Herron, Hiromi Sesaki, Seema Khurana
BACKGROUND & AIMS: Cell stress signaling pathways result in phosphorylation of the eukaryotic translation initiation factor 2 subunit alpha (EIF2S1 or EIF2A), which affects regulation of protein translation. Translation reprogramming mitigates stress by activating pathways that result in autophagy and cell death, to eliminate damaged cells. Actin is modified during stress and EIF2A is dephosphorylated to restore homeostasis. It is not clear how actin affects EIF2A signaling. We studied the actin-binding proteins villin 1 (VIL1) and gelsolin (GSN) in intestinal epithelial cells (IECs) to determine whether they respond to cell stress response and affect signaling pathways...
December 21, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29186150/in-vitro-enteroid-derived-three-dimensional-tissue-model-of-human-small-intestinal-epithelium-with-innate-immune-responses
#9
Ying Chen, Wenda Zhou, Terrence Roh, Mary K Estes, David L Kaplan
There is a need for functional in vitro 3D human intestine systems that can bridge the gap between conventional cell culture studies and human trials. The successful engineering in vitro of human intestinal tissues relies on the use of the appropriate cell sources, biomimetic scaffolds, and 3D culture conditions to support vital organ functions. We previously established a compartmentalized scaffold consisting of a hollow space within a porous bulk matrix, in which a functional and physiologically relevant intestinal epithelium system was generated using intestinal cell lines...
2017: PloS One
https://www.readbyqxmd.com/read/29178040/live-imaging-analysis-of-human-gastric-epithelial-spheroids-reveals-spontaneous-rupture-rotation-and-fusion-events
#10
T Andrew Sebrell, Barkan Sidar, Rachel Bruns, Royce A Wilkinson, Blake Wiedenheft, Paul J Taylor, Brian A Perrino, Linda C Samuelson, James N Wilking, Diane Bimczok
Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-dimensional culture matrix. Therefore, we performed extended time-lapse imaging analysis (up to 4 days) of human gastric epithelial spheroids generated from adult tissue samples in order to visualize the dynamics of the spheroids in detail...
November 25, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29144770/equine-intestinal-mucosal-pathobiology
#11
Anthony Blikslager, Liara Gonzalez
The equine intestinal mucosa is intimately involved in maintaining homeostasis both on a systemic level by controlling extracellular fluid movement and at the local level to maintain barrier function. Horses are particularly susceptible to the clinical syndrome of colic, with the most severe cases involving strangulating obstruction that induces ischemia. Because of the mucosal vascular architecture, the mucosal epithelium is particularly susceptible to ischemic injury. The potential for reperfusion injury has been investigated and found to play a minimal role...
November 16, 2017: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/29142770/placental-teratoma-omphalomesenteric-duct-remnant-or-intestinal-organoid-enteroid-differentiation-a-diagnostic-dilemma
#12
Salwa Khedr, Tarek Jazaerly, Stefan Kostadinov
We report an unusual case of fully developed fetal intestinal segment(s) within a nodule on the chorionic plate of the placenta of a 27-year-old female patient at 37 weeks gestation with spontaneous vaginal delivery. Gross examination of the placenta revealed a chorionic plate nodule near the insertion of the umbilical cord, which, upon microscopic evaluation, raised the differential diagnostic possibilities of placental teratoma, vitelline/omphalomesenteric duct anomaly, and intestinal organoid differentiation...
December 2017: Journal of Pediatric Genetics
https://www.readbyqxmd.com/read/29129972/inflammation-and-colorectal-cancer
#13
Apple G Long, Emma T Lundsmith, Kathryn E Hamilton
Colorectal cancer (CRC) is the fourth most common cancer in both men and women in the United States, resulting in over 55,000 deaths annually. Environmental and genetic factors influence the development of CRC, and inflammation is a critical hallmark of cancer that may arise from a variety of factors. Purpose of review: While patients with inflammatory bowel disease (IBD) have a higher risk of developing CRC, sporadic CRCs may engender or be potentiated by inflammation as well. In this review, we focus on recent advances in basic and translational research utilizing murine models to understand the contribution of inflammatory signaling pathways to CRC...
August 2017: Current Colorectal Cancer Reports
https://www.readbyqxmd.com/read/29109476/the-major-targets-of-acute-norovirus-infection-are-immune-cells-in-the-gut-associated-lymphoid-tissue
#14
Katrina R Grau, Alexa N Roth, Shu Zhu, Abel Hernandez, Natacha Colliou, Bayli B DiVita, Drake T Philip, Cara Riffe, Benoit Giasson, Shannon M Wallet, Mansour Mohamadzadeh, Stephanie M Karst
Noroviruses are the leading cause of food-borne gastroenteritis outbreaks and childhood diarrhoea globally, estimated to be responsible for 200,000 deaths in children each year 1-4 . Thus, reducing norovirus-associated disease is a critical priority. Development of vaccines and therapeutics has been hindered by the limited understanding of basic norovirus pathogenesis and cell tropism. While macrophages, dendritic cells, B cells and stem-cell-derived enteroids can all support infection of certain noroviruses in vitro 5-7 , efforts to define in vivo norovirus cell tropism have generated conflicting results...
December 2017: Nature Microbiology
https://www.readbyqxmd.com/read/29030247/inhibition-of-intestinal-ascorbic-acid-uptake-by-lipopolysaccharide-is-mediated-via-transcriptional-mechanism-s
#15
Veedamali S Subramanian, Subrata Sabui, Hamid Moradi, Jonathan S Marchant, Hamid M Said
Ascorbic acid (AA) accumulation in intestinal epithelial cells is an active transport process mainly mediated by two sodium-dependent vitamin C transporters (SVCT-1 and SVCT-2). To date, little is known about the effect of gut microbiota generated lipopolysaccharide (LPS) on intestinal absorption of water-soluble vitamins. Therefore, the objective of this study was to investigate the effects of bacterially-derived LPS on AA homeostasis in enterocytes using Caco-2 cells, mouse intestine and intestinal enteroids models...
October 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28958857/the-function-of-atpase-copper-transporter-atp7b-in-intestine
#16
Hannah Pierson, Abigael Muchenditsi, Byung-Eun Kim, Martina Ralle, Nicholas Zachos, Dominik Huster, Svetlana Lutsenko
BACKGROUND & AIMS: Wilson disease is a disorder of copper (Cu) misbalance caused by mutations in ATP7B. ATP7B is highly expressed in the liver-the major site of Cu accumulation in patients with Wilson disease. The intestine also expresses ATP7B, but little is known about the contribution of intestinal ATP7B to normal intestinal homeostasis or to Wilson disease manifestations. We characterized the role of ATP7B in mouse intestinal organoids and tissues. METHODS: We collected intestinal tissues from ATP7B-knockout (Atp7b(-/-)) and control mice, and established 3-dimensional enteroids...
September 25, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28934294/immunopathology-of-childhood-celiac-disease-key-role-of-intestinal-epithelial-cells
#17
Grzegorz Pietz, Rituparna De, Maria Hedberg, Veronika Sjöberg, Olof Sandström, Olle Hernell, Sten Hammarström, Marie-Louise Hammarström
BACKGROUND & AIMS: Celiac disease is a chronic inflammatory disease of the small intestine mucosa due to permanent intolerance to dietary gluten. The aim was to elucidate the role of small intestinal epithelial cells in the immunopathology of celiac disease in particular the influence of celiac disease-associated bacteria. METHODS: Duodenal biopsies were collected from children with active celiac disease, treated celiac disease, and clinical controls. Intestinal epithelial cells were purified and analyzed for gene expression changes at the mRNA and protein levels...
2017: PloS One
https://www.readbyqxmd.com/read/28929461/the-isolation-culture-and-propagation-of-murine-intestinal-enteroids-for-the-study-of-dietary-lipid-metabolism
#18
Diana Li, Hongli Dong, Alison B Kohan
Since the initial report in 2009 by Sato and Clevers, primary enteroids have been of major interest in the fields of stem cell biology and gastrointestinal (GI) tract biology. More recently, we and others have made major inroads into the physiological relevance of these enteroid models and have shown that enteroids derived from the stomach, intestine, or colon recapitulate major functions of these tissues, namely, gastric acid secretion, lipid absorption and lipoprotein secretion, and ion transport. Here, we detail the isolation of stem cells from the small intestine and the culture and propagation of those stem cells into mature three-dimensional enteroids...
September 20, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28912250/role-of-microrna-423-5p-in-posttranscriptional-regulation-of-the-intestinal-riboflavin-transporter-3
#19
Ram Lakhan, Veedamali S Subramanian, Hamid M Said
Riboflavin (RF) is essential for normal cellular functions and health. Humans obtain RF from exogenous sources via intestinal absorption that involves a highly specific carrier-mediated process. We have recently established that the riboflavin transporter-3 (RFVT3) is vital for the normal intestinal RF uptake process and have characterized certain aspects of its transcriptional regulation. Little is known, however, about how this transporter is regulated at the posttranscriptional level. We address this issue by focusing on the role of microRNAs...
December 1, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28854151/aging-effects-on-intestinal-homeostasis-associated-with-expansion-and-dysfunction-of-intestinal-epithelial-stem-cells
#20
Emily C Moorefield, Sarah F Andres, R Eric Blue, Laurianne Van Landeghem, Amanda T Mah, M Agostina Santoro, Shengli Ding
Intestinal epithelial stem cells (IESCs) are critical to maintain intestinal epithelial function and homeostasis. We tested the hypothesis that aging promotes IESC dysfunction using old (18-22 months) and young (2-4 month) Sox9-EGFP IESC reporter mice. Different levels of Sox9-EGFP permit analyses of active IESC (Sox9-EGFP(Low)), activatable reserve IESC and enteroendocrine cells (Sox9-EGFP(High)), Sox9-EGFP(Sublow) progenitors, and Sox9-EGFP(Negative) differentiated lineages. Crypt-villus morphology, cellular composition and apoptosis were measured by histology...
August 29, 2017: Aging
keyword
keyword
45547
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"