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Eung-goo Lee, Jae-hoon Kim, Yong-seung Shin, Gee-wook Shin, Young-rim Kim, K J Palaksha, Dae-yong Kim, Itsuro Yamane, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung
Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and M(r) between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N...
February 5, 2005: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yuko Uchida, Kazunori Ike, Tetsuro Kurotaki, Midori Takeshi, Soichi Imai
Djungarian hamsters were examined for the susceptibility to Neospora caninum infection. After 29 Djungarian hamsters were intraperitoneally inoculated with 5 x 10(6) N. caninum tachyzoites of JPA1 strain, some animals showed symptoms such as ataxia, and many tissue cysts were detected in the brain and a cyst in the muscular tunics of stomach. Especially, more than 100 cysts per head were observed after 5 weeks post inoculation. It is suggested that the Djungarian hamster is a model useful to examine neosporosis...
March 2003: Journal of Veterinary Medical Science
T Shibahara, T Kokuho, M Eto, M Haritani, T Hamaoka, K Shimura, K Nakamura, Y Yokomizo, I Yamane
Neospora is a cyst-forming coccidian parasite that causes abortions and neuromuscular disorders in a wide variety of mammals. Japanese bovine isolate JPA1 was inoculated intraperitoneally into BALB/c nu/ nu (athymic nude) and BALB/c (congenic wild type) female mice to examine the distribution of parasites and resistance mechanisms to Neospora infection. All the athymic nude mice died within 28 days after intraperitoneal injection of 2 x 10(5) JPA1 tachyzoites, whereas all the congenic wild type mice survived without exhibiting any clinical signs...
July 1999: Veterinary Pathology
I Yamane, T Kokuho, K Shimura, M Eto, T Shibahara, M Haritani, Y Ouchi, K Sverlow, P A Conrad
Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37 degrees C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera...
July 1997: Research in Veterinary Science
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