Masaya Koganesawa, Daniel F Dwyer, Kinan Alhallak, Jun Nagai, Kendall Zaleski, Sachin Samuchiwal, Hayashi Hiroaki, Airi Nishida, Thomas I Hirsch, Patrick J Brennan, Mark Puder, Barbara Balestrieri
Macrophages expressing group V phospholipase A2 (Pla2g5) release the free fatty acid (FFA) linoleic acid (LA), potentiating lung type 2 inflammation. Although Pla2g5 and LA increase in viral infections, their role remains obscure. We generated Pla2g5flox/flox mice, deleted Pla2g5 by using the Cx3cr1cre transgene, and activated bone marrow-derived macrophages (BM-Macs) with poly:IC, a synthetic double-stranded RNA that triggers a viral-like immune response, known Pla2g5-dependent stimuli (IL-4, LPS + IFNγ, IL-33 + IL-4 + GM-CSF) and poly:IC + LA followed by lipidomic and transcriptomic analysis...
February 15, 2024: Immunology