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https://www.readbyqxmd.com/read/29618666/peptide-vaccine-immunotherapy-biomarkers-and-response-patterns-in-pediatric-gliomas
#1
Sören Müller, Sameer Agnihotri, Karsen E Shoger, Max I Myers, Nicholas Smith, Srilakshmi Chaparala, Clarence R Villanueva, Ansuman Chattopadhyay, Adrian V Lee, Lisa H Butterfield, Aaron Diaz, Hideho Okada, Ian F Pollack, Gary Kohanbash
Low-grade gliomas (LGGs) are the most common brain tumor affecting children. We recently reported an early phase clinical trial of a peptide-based vaccine, which elicited consistent antigen-specific T cell responses in pediatric LGG patients. Additionally, we observed radiologic responses of stable disease (SD), partial response (PR), and near-complete/complete response (CR) following therapy. To identify biomarkers of clinical response in peripheral blood, we performed RNA sequencing on PBMC samples collected at multiple time points...
April 5, 2018: JCI Insight
https://www.readbyqxmd.com/read/29605790/fatal-varicella-in-immigrants-from-tropical-countries-case-reports-and-forensic-perspectives
#2
Gianni Guadagnini, Simone Lo Baido, Francesca Poli, Annamaria Govi, Sveva Borin, Paolo Fais, Susi Pelotti
The primary Varicella Zoster Virus (VZV) infection results in varicella, a generally benign, self-limiting disease in immunocompetent children. Despite the usual course a possible fatal evolution of the primary infection is observed predominantly in immunocompromised subjects and in adults, especially emigrating from tropical regions. Two cases of fatal varicella have been investigated and discussed. Death occurred in two patients over 40 years of age, coming from South Asia and receiving chronic immunosuppressive therapy...
March 24, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29605181/characterization-of-new-polymer-grafted-protein-cation-exchangers-developed-by-partial-neutralization-of-carboxyl-groups-derivatized-by-modification-of-poly-ethylenimine-sepharose-with-succinic-anhydride
#3
Yangyang Zhao, Xiaoyan Dong, Linling Yu, Yang Liu, Yan Sun
Previously, we have studied protein adsorption and chromatographic behaviors on poly(ethylenimine) (PEI)-grafted Sepharose FF anion-exchange resins, and found that protein uptake rates increased greatly when PEI grafting density reached over a critical ionic capacity (cIC) due to the occurrence of the "chain delivery" effect. Moreover, by partial charge neutralization of starting resin FF-PEI-L740 (IC = 740 mmol/L, larger than the cIC) with sodium acetate to FF-PEI-R440, it exhibited a three-fold increase in uptake rate over FF-PEI-L740...
March 23, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29600844/click-chemical-ligation-initiated-on-bead-dna-polymerization-for-the-sensitive-flow-cytometric-detection-of-3%C3%A2-terminal-2%C3%A2-o-methylated-plant-microrna
#4
Wenjiao Fan, Yan Qi, Liying Qiu, Pan He, Chenghui Liu, Zhengping Li
A versatile flow cytometric strategy is developed for the sensitive detection of plant microRNA (miRNA) by coupling the target-templated click nucleic acid ligation (CNAL) with on-bead terminal enzymatic DNA polymerization (TEP). Unlike ligase-catalyzed ligation reaction, the plant miRNA-templated enzyme-free CNAL between two single-stranded DNA (ssDNA) probes respectively modified with Aza-dibenzocyclooctyne (Aza-DBCO) and N3, can not only simplify the operation but also achieve a much higher ligation efficiency...
March 30, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29597033/nevirapine-polycaprolactone-crystalline-inclusion-complex-as-a-potential-long-acting-injectable-solid-form
#5
Xiaotong Yang, Beihang Yu, Zhi Zhong, Bao-Hua Guo, Yanbin Huang
Nevirapine (NVP) is recommended by WHO as the antiretroviral treatment to prevent HIV passing from mother to child. However, the once-daily oral administration results in poor patient compliance, and a long-acting injectable form of NVP is desirable. Using single-crystal X-ray diffraction and other characterization methods, we demonstrated NVP can form crystalline inclusion complex (IC) with the biodegradable hydrophobic poly(ε-caprolactone) (PCL), and investigated the potential of the NVP-PCL IC microparticles as a long-acting injectable solid form...
March 26, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29570417/reduction-of-marek-s-disease-virus-infection-by-toll-like-receptor-ligands-in-chicken-embryo-fibroblast-cells
#6
Jegarubee Bavananthasivam, Raveendra R Kulkarni, Leah Read, Shayan Sharif
Evolutionarily conserved pattern recognition receptors, including Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) that are present in microbes. PAMPs induce several pathways downstream of TLRs that lead to induction of antiviral responses. The objective of this study was to investigate the stimulatory effect of various PAMPs (in the form of TLR ligands) in reducing Marek's disease virus (MDV) infection in chicken embryo fibroblast cells (CEFs). To this end, CEFs were pretreated with Pam3CSK4, Poly(IC), lipopolysaccharide (LPS), and CpG ODN as TLR2, TLR3, TLR4, and TLR21 ligands, respectively for 24 h followed by infection with MDV...
March 23, 2018: Viral Immunology
https://www.readbyqxmd.com/read/29519508/modulation-of-type-i-interferon-signaling-by-african-swine-fever-virus-asfv-of-different-virulence-l60-and-nhv-in-macrophage-host-cells
#7
Raquel Portugal, Alexandre Leitão, Carlos Martins
ASFV causes an important disease of domestic swine and wild boar. Currently no vaccine is available, highlighting the necessity to understand ASFV modulation of innate immune responses in natural host cells. With this aim, macrophage cultures enriched in SWC9 and CD163 differentiation markers were infected in parallel with high virulent ASFV/L60 and low virulent ASFV/NHV, the latter lacking MGF 360 and 505/530 genes associated with type I interferon (IFN I) control. IFN I production and signaling were studied after completion of the viral cycles...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29483127/sustained-persistence-of-il2-signaling-enhances-the-antitumor-effect-of-peptide-vaccines-through-t-cell-expansion-and-preventing-pd-1-inhibition
#8
Hussein Sultan, Takumi Kumai, Valentyna I Fesenkova, Aaron E Fan, Juan Wu, Hyun-Il Cho, Hiroya Kobayashi, Yasuaki Harabuchi, Esteban Celis
Peptide vaccines can be a successful and cost-effective way of generating T-cell responses against defined tumor antigens, especially when combined with immune adjuvants such as poly-IC. However, strong immune adjuvants can induce a collateral increase in numbers of irrelevant, non-specific T cells, which limits the effectiveness of the peptide vaccines. Here we report that providing prolonged IL2 signaling either in the form of IL2/anti-IL2 complexes or pegylated IL2 overcomes the competitive suppressive effect of irrelevant T cells, allowing the preferential expansion of antigen-specific T cells...
February 26, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29465176/nurses-experiences-of-providing-care-to-bereaved-families-who-experience-unexpected-death-in-intensive-care-units-a-narrative-overview
#9
Aalia Shariff, Joanne Olson, Anna Santos Salas, Lisa Cranley
BACKGROUND: Death is a common occurrence in intensive care units (IC Us) and the complexity of care makes it difficult for nurses to find a balance between the patient's physical needs and the family's emotional needs, especially in circumstances of unexpected death. Cumulative or unresolved grief for families can have lasting negative repercussions. Nurses, therefore, need access to bereavement education in order to provide optimal- bereavement support. PURPOSE: The purpose of this review is to identify challenges and facilitators that nurses experience in delivering bereavement support during and after sudden or unexpected death in ICUs...
May 2017: Canadian Journal of Critical Care Nursing
https://www.readbyqxmd.com/read/29449668/rhinovirus-3c-protease-suppresses-apoptosis-and-triggers-caspase-independent-cell-death
#10
Mark Lötzerich, Pascal S Roulin, Karin Boucke, Robert Witte, Oleg Georgiev, Urs F Greber
Apoptosis and programmed necrosis (necroptosis) determine cell fate, and antagonize infection. Execution of these complementary death pathways involves the formation of receptor-interacting protein kinase 1 (RIPK1) containing complexes. RIPK1 binds to adaptor proteins, such as TRIF (Toll-IL-1 receptor-domain-containing-adaptor-inducing interferon-beta factor), FADD (Fas-associated-protein with death domain), NEMO (NF-κB regulatory subunit IKKγ), SQSTM1 (sequestosome 1/p62), or RIPK3 (receptor-interacting protein kinase 3), which are involved in RNA sensing, NF-κB signaling, autophagosome formation, apoptosis, and necroptosis...
February 15, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29389566/a-novel-poly-glycine-biosensor-towards-the-detection-of-indigo-carmine-a-voltammetric-study
#11
Jamballi Gangadharappa Gowda Manjunatha
The electrochemical behavior of indigo carmine (IC) at poly (glycine) modified carbon paste electrode (PGMCPE) was investigated by cyclic and differential pulse voltammetry. The oxidation peak of IC was observed in phosphate buffer of pH 6.5. The influence of different pH, scan rate, and concentration were analyzed. The probable reaction mechanism involved in the oxidation of IC was also proposed. Results showed that PGMCPE a remarkable electrocatalytic activity for the oxidation of IC under optimal conditions...
January 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29358709/tumor-derived-exosomes-antagonize-innate-antiviral-immunity
#12
Liang Gao, Lin Wang, Tong Dai, Ke Jin, Zhengkui Zhang, Shuai Wang, Feng Xie, Pengfei Fang, Bing Yang, Huizhe Huang, Hans van Dam, Fangfang Zhou, Long Zhang
Malignancies can compromise innate immunity, but the mechanisms of this are largely unknown. Here we found that, via tumor-derived exosomes (TEXs), cancers were able to transfer activated epidermal growth factor receptor (EGFR) to host macrophages and thereby suppress innate antiviral immunity. Screening of the human kinome identified the kinase MEKK2 in macrophages as an effector of TEX-delivered EGFR that negatively regulated the antiviral immune response. In the context of experimental tumor implantation, MEKK2-deficient mice were more resistant to viral infection than were wild-type mice...
March 2018: Nature Immunology
https://www.readbyqxmd.com/read/29342494/how-does-the-patient-react-after-reading-the-informed-consent-form-of-a-gynecological-surgery-a-qualitative-study
#13
Andrea Cristina Amorim, Luis Guilherme Teixeira Dos Santos, Omero Benedicto Poli-Neto, Luiz Gustavo Oliveira Brito
OBJECTIVE:  To analyze the reaction of women after reading the Informed Consent Form (ICF) before undergoing elective gynecological/urogynecological surgeries. METHODS:  A qualitative study with 53 women was conducted between September 2014 and May 2015. The analysis of the content was conducted after a scripted interview was made in a reserved room and transcribed verbatim. We read the ICF once more in front of the patient, and then she was interviewed according to a script of questions about emotions and reactions that occurred about the procedure and her expectations about the intra- and postoperative period...
January 17, 2018: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/29314839/interplay-between-hydroxyl-density-and-relaxations-in-poly-vinylbenzyltrimethylammonium-b-poly-methylbutylene-membranes-for-electrochemical-applications
#14
Keti Vezzù, Ashley M Maes, Federico Bertasi, Andrew R Motz, Tsung-Han Tsai, E Bryan Coughlin, Andrew M Herring, Vito Di Noto
Anion-exchange membranes (AEMs) consisting of poly(vinyl benzyl trimethylammonium)-b-poly(methylbutylene) of three different ion exchange capacities (IECs), 1.14, 1.64, and 2.03 mequiv g-1 , are studied by High-Resolution Thermogravimetry, Modulated Differential Scanning Calorimetry, Dynamic Mechanical Analysis, and Broadband Electrical Spectroscopy in their OH- form. The thermal stability and transitions are elucidated, showing a low temperature Tg and a higher temperature transition assigned to a disorder-order transition, Tδ , which depends on the IEC of the material...
January 31, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29280358/thermoresponsive-supramolecular-chemotherapy-by-v-shaped-armed-%C3%AE-cyclodextrin-star-polymer-to-overcome-drug-resistance
#15
Xiaoshan Fan, Hongwei Cheng, Xiaoyuan Wang, Enyi Ye, Xian Jun Loh, Yun-Long Wu, Zibiao Li
Pump mediated drug efflux is the key reason to result in the failure of chemotherapy. Herein, a novel star polymer β-CD-v-(PEG-β-PNIPAAm)7 consisting of a β-CD core, grafted with thermo-responsive poly(N-isopropylacrylamide) (PNIPAAm) and biocompatible poly(ethylene glycol) (PEG) in the multiple "V"-shaped arms is designed and further fabricated into supramolecular nanocarriers for drug resistant cancer therapy. The star polymer could encapsulate chemotherapeutics between β-cyclodextrin and anti-cancer drug via inclusion complex (IC)...
December 27, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29276185/non-viable-lactobacillus-casei-beneficially-modulates-poly-i-c-immune-response-in-co-cultures-of-human-cells
#16
Elisa Vintiñi, Marcela Medina
BACKGROUND: Polyinosinic:polycytidylic acid (Poly-IC) has been used as a viral stimulus to mimic in vivo and in vitro infection induced by some viruses. OBJECTIVE: To determine whether non-viable Lactobacillus casei CRL431 (LcM) can modulate the immune response induced by Poly I:C in co-culture models of peripheral blood mononuclear cells (PBMC) and A549 cells. METHODS: T and NK cell activation was evaluated by flow cytometry and levels of TNF-α, IFN-γ, IL-10, IL-29, and IL-17 by ELISA...
December 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/29249322/saponin-quil-a-up-regulates-type-i-interferon-regulated-gene-and-type-i-and-ii-interferon-expressions-which-are-suppressed-by-porcine-reproductive-and-respiratory-syndrome-virus
#17
Wasin Charerntantanakul, Dante Fabros
Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses innate immune response following infection of myeloid antigen-presenting cells. Poor innate immune response results in weak and delayed PRRSV-specific adaptive immunity, and facilitates PRRSV replication, pathogenesis, and persistent infection. Numerous efforts have been made to enhance the effective innate and adaptive immune defenses to PRRSV, however, only a few attempts have so far elicited satisfactory results. The present study aims to evaluate in vitro the potential of saponin quil A to enhance the expression of type I interferon (IFN)-regulated gene, type I and II IFNs, and pro-inflammatory cytokines in PRRSV-inoculated peripheral blood mononuclear cells (PBMC)...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29204085/immune-complex-negatively-regulates-toll-like-receptor-3-triggered-tumour-necrosis-factor-%C3%AE-production-in-b-cells
#18
Li Qian, Wenyan Chen, Shaoqing Wang, Yang Liu, Xiaoqin Jia, Yi Fu, Weijuan Gong, Fang Tian
Inappropriate activation of toll-like receptor 3 (TLR3) has been implicated in the pathogenesis of autoimmune diseases, so the negative regulation of TLR3-triggered immune response has received increasing attention. Nonpathogenic immune complex (IC) has been used as treatment for many inflammatory and autoimmune diseases. However, the role of IC in the regulation of TLR3-triggered immune responses and the underlying mechanisms need to be investigated. In this study we demonstrate that IC or intravenous immunoglobulin (Ig) stimulation of B cells attenuates polyinosinic:polycytidylic acid (poly I:C)-induced CD40 expression; IC, but not Ig, can significantly inhibit poly I:C-induced pro-inflammatory tumour necrosis factor α (TNF-α) production by B cells...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/29166644/cylindromatosis-cyld-a-deubiquitinase-attenuates-inflammatory-signaling-pathways-by-activating-toll-like-receptor-3-in-human-mesangial-cells
#19
Tadaatsu Imaizumi, Ryo Hayakari, Shojiro Watanabe, Tomomi Aizawa, Tomoh Matsumiya, Hidemi Yoshida, Kazushi Tsuruga, Shogo Kawaguchi, Hiroshi Tanaka
BACKGROUND/AIMS: Cylindromatosis (CYLD), a deubiquitinase, negatively regulates nuclear factor-κB in various cells. However, its potential roles in glomerular inflammation remain unclear. Because the activation of the Toll-like receptor 3 (TLR3)/type I interferon (IFN) pathways plays a pivotal role in chronic kidney diseases (CKD), we examined the role of CYLD in the TLR3 signaling in cultured human mesangial cells (MCs). METHODS: We stimulated CYLD-silenced MCs with polyinosinic-polycytidylic acid (poly IC), a synthetic analogue of dsRNA, and studied representative TLR3/IFN-β pathways (i...
2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29130803/steroid-sparing-effect-of-sublingual-immunotherapy-real-life-study-in-mono-polisensitized-children-with-asthma
#20
Nerin Nadir Bahceciler, Nilufer Galip, Arzu Babayigit
AIM: Steroid-sparing effect of sublingual immunotherapy (SLIT) in mono/polisensitized asthmatic children were evaluated. MATERIALS AND METHODS:  Children undergoing allergen-specific SLIT between 2010 and 2014 were included. Asthma control and usage/dose of inhaled corticosteroid (ICS) in the previous year was determined. Asthma control without ICS need ≥6 months was defined as 'ICS avoidance'. RESULTS:  90 children (mean ± SD age 8...
November 2017: Immunotherapy
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