Read by QxMD icon Read

Poly ic

Yushu Yin, Georgia Papavasiliou, Olga Y Zaborina, John C Alverdy, Fouad Teymour
The human gastrointestinal tract is the primary site of colonization of multidrug resistant pathogens and the major source of life-threatening complications in critically ill and immunocompromised patients. Eradication measures using antibiotics carry further risk of antibiotic resistance. Furthermore, antibiotic treatment can adversely shift the intestinal microbiome toward domination by resistant pathogens. Therefore, approaches directed to prevent replacement of health promoting microbiota with resistant pathogens should be developed...
October 19, 2016: Annals of Biomedical Engineering
F Salazar Meira, J Zemiacki, A E Figueiredo, L Viliano Kroth, D Saute Kochhann, D O d'Avila, M Traesel, D Saitovitch, C E Poli-de-Figueiredo
BACKGROUND: One of the main postoperative complications of kidney transplant is delayed graft function (DGF), which means absence of graft function after transplant or the need for dialysis during the first week post procedure. The occurrence of DGF currently in our hospital is high and has been attributed to a combination of many factors. The aim of this study was to evaluate the factors associated to DGF and their influence in the outcome of kidney transplants. METHODS: Historical cohort of 150 patients transplanted with live or deceased donor kidneys from 2011 to 2013...
September 2016: Transplantation Proceedings
Briana R Dye, Priya H Dedhia, Alyssa J Miller, Melinda S Nagy, Eric S White, Lonnie D Shea, Jason R Spence
Human pluripotent stem cell (hPSC) derived tissues often remain developmentally immature in vitro, and become more adult-like in their structure, cellular diversity and function following transplantation into immunocompromised mice. Previously we have demonstrated that hPSC-derived human lung organoids (HLOs) resembled human fetal lung tissue in vitro (Dye et al. 2015). Here we show that HLOs required a bioartificial microporous Poly(lactide-co-glycolide) (PLG) scaffold niche for successful engraftment, long-term survival, and maturation of lung epithelium in vivo...
September 28, 2016: ELife
Omero Benedicto Poli-Neto, Paula Patrícia de Freitas Chama, Adriana Peterson Mariana Salata Romão, Rosana Aparecida Spadoti Dantas, Júlio Cesar Rosa-E-Silva, Francisco Jose Candido-Dos-Reis, Antonio Alberto Nogueira
Objectives to translate and adapt the Patient Satisfaction Questionnaire (PSQ) to Portuguese and to assess its psychometric properties based on internal consistency, test-retest, factor analysis and divergent and convergent construct validities. Methods The study involved 218 participants and was approved by the local Research Ethics Committee. All participants gave written informed consent and their anonymity was ensured. The instrument was translated and culturally adapted for use in the Portuguese language...
September 19, 2016: Revista Brasileira de Ginecologia e Obstetrícia
Tadaatsu Imaizumi, Chikashi Yano, Akiko Numata, Koji Tsugawa, Ryo Hayakari, Tomoh Matsumiya, Hidemi Yoshida, Shojiro Watanabe, Kazushi Tsuruga, Shogo Kawaguchi, Manabu Murakami, Hiroshi Tanaka
BACKGROUND/AIMS: Activation of Toll-like receptor 3 (TLR3) signaling followed by type I interferon (IFN) expression is crucial in antiviral and "pseudoviral" immune reactions in renal mesangial cells (MCs). These reactions are probably involved in the pathogenesis of chronic kidney disease (CKD). However, the role of IFN-induced 35-kDa protein 35 (IFI35), a type I IFN-dependent transcript, in glomerular inflammation is unclear. Here, we aimed to investigate the expression and the role of IFI35 in IFN-β/retinoic acid-inducible gene-I (RIG-I)/CCL5 and IFN-β/melanoma differentiation-associated gene 5 (MDA5)/CXCL10 axes in MCs...
September 17, 2016: Kidney & Blood Pressure Research
Douglas C Wu, Mitchel P Goldman
BACKGROUND: The risk of nodule formation following poly-L-lactic acid (PLLA) injections for facial volume loss is well known. Traditionally, post-treatment massage according to the 5-5-5 rule (5 times per day for 5 minutes for 5 days) has been applied to mitigate this risk. However, such a regimen may be onerous for patient compliance. Using currently accepted injection technique and product dilution, the efficacy of massage for nodule prevention has never been formally evaluated. OBJECTIVE: To evaluate the efficacy of massage in reducing the incidence of nodule formation post-PLLA injection...
September 9, 2016: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Daniel M Houslay, Karen E Anderson, Tamara Chessa, Suhasini Kulkarni, Ralph Fritsch, Julian Downward, Jonathan M Backer, Len R Stephens, Phillip T Hawkins
Class I phosphoinositide 3-kinases (PI3Ks) catalyze production of the lipid messenger phosphatidylinositol 3,4,5-trisphosphate (PIP3), which plays a central role in a complex signaling network regulating cell growth, survival, and movement. This network is overactivated in cancer and inflammation, and there is interest in determining the PI3K catalytic subunit (p110α, p110β, p110γ, or p110δ) that should be targeted in different therapeutic contexts. Previous studies have defined unique regulatory inputs for p110β, including direct interaction with Gβγ subunits, Rac, and Rab5...
2016: Science Signaling
Yoon Chung Kim, Young Hoon Kim, Jang Woon Kim, Kee Yong Ha
Experimental stem cell therapy for spinal cord injury (SCI) has been extensively investigated. The selection of effective cell transplantation route is also an important issue. Although various types of scaffold have been widely tried as a carrier of stem cells to the injured spinal cord, there was little comparative study to investigate the efficacy of transplantation comparing with conventional transplantation route. A total of 48 Sprague-Dawley rats were subjected to standardized SCI, followed by transplantation of allogeneic mesenchymal stem cells (MSCs), either via intralesional injection (IL group), or via the poly (lactic-co-glycolic acid) (PLGA) scaffold (IP group) or chitosan scaffold (IC group)...
September 2016: Journal of Korean Medical Science
Ariane Boumendil, Maguy Woimant, Jean-Pierre Quenot, François-Xavier Rooryck, Foued Makhlouf, Youri Yordanov, Samuel Delerme, Khalil Takun, Patrick Ray, Marie-Clément Kouka, Claire Poly, Maité Garrouste-Orgeas, Caroline Thomas, Tabasome Simon, Sylvie Azerad, Guillaume Leblanc, Dominique Pateron, Bertrand Guidet
BACKGROUND: The benefit of ICU admission for elderly patients remains controversial. This report highlights the methodology, the feasibility of and the ethical and logistical constraints in designing and conducting a cluster-randomized trial of intensive care unit (ICU) admission for critically ill elderly patients. METHODS: We designed an interventional open-label cluster-randomized controlled trial in 24 centres in France. Clusters were healthcare centres with at least one emergency department (ED) and one ICU...
December 2016: Annals of Intensive Care
Cristina Lucidi, Stefano Ginanni Corradini, Juan G Abraldes, Manuela Merli, Puneeta Tandon, Flaminia Ferri, Lucia Parlati, Barbara Lattanzi, Edoardo Poli, Vincenza Di Gregorio, Alessio Farcomeni, Oliviero Riggio
Despite its documented prognostic relevance, hepatic encephalopathy (HE) is not considered in liver transplantation (LT) due to its possible poor objectivity. To override this problem, we aimed to analyze if an objective diagnosis of HE may confer additional mortality risk beyond MELD. Study and validation cohorts of patients with cirrhosis were considered in Italy and Canada, respectively. Patients were considered to be HE+ if an episode of overt HE was documented in a hospitalization. Of the 486 patients enrolled in Italy, 184 (38%) were HE+...
October 2016: Liver Transplantation
Seung Bum Park, Scott Seronello, Wasima Mayer, David M Ojcius
Hepatitis C virus (HCV) actively evades host interferon (IFN) responses but the mechanisms of how it does so are not completely understood. In this study, we present evidence for an HCV factor that contributes to the suppression of retinoic-acid-inducible gene-I (RIG-I)-mediated IFN induction. Expression of frameshift/alternate reading frame protein (F/ARFP) from HCV -2/+1 frame in Huh7 hepatoma cells suppressed type I IFN responses stimulated by HCV RNA pathogen-associated molecular pattern (PAMP) and poly(IC)...
2016: PloS One
Tomas Garzon-Muvdi, Antonella Mangraviti, Debebe Theodros, Eileen Kim, Michael Jay Yellin, Henry Marsh, Michael Lim
INTRODUCTION: FLT3L induces expansion and recruitment of plasmacytoid dendritic cells (pDCs) into the brain. By increasing antigen presentation, FLT3L agonists may enhance an immune response against glioblastoma (GBM). Previous results suggest that the antitumor response generated by FLT3 is secondary to production of interferon gamma. We studied the effect of FLT3L treatment of mice with a syngeneic GBM model. METHODS: Under ACUC approval, 32 mice underwent implantation of 130 000 GL261 cells in the left striatum using a stereotactic frame...
August 2016: Neurosurgery
Zhao-Feng Xie, Shu-Shui Wang, Zhi-Wei Zhang, Jian Zhuang, Xiang-Dong Liu, Xian-Miao Chen, Gui Zhang, Deyuan Zhang
OBJECTIVES: The aim of this study is to evaluate the long-term effectiveness and safety of a self-expandable, double-disk biodegradable device made of poly-L-lactic acid (PLLA) for closure of atrial septal defects (ASDs) in swine. METHODS: ASDs were created by transseptal needle puncture followed by balloon dilatation in 20 piglets. The experimental group comprised 18 animals, while the remaining 2 animals were used as controls. Effectiveness and safety were evaluated by rectal temperature, leukocyte count, chest radiography, electrocardiogram, transthoracic echocardiography (TTE), intracardiac echocardiography (ICE), and histologic studies...
July 12, 2016: Cardiology
Hina Rehman, Safila Naveed, Khan Usmanghani
To evaluate the safety and efficacy of Linkus, Aminophylline with Diphenhydramine group and Acefyllin Piperazine with Diphenhydramine cough syrup on children having cough and sleep difficulty associated with cough. To determine the effects of Linkus polyherbal syrup (group A) and compared with other parallel allopathic groups (Group B and C) for cough on children and associated sleep quality and improvement. 360 children having cough inducted in 3 different groups randomly selected. Three parallel groups were the part of the study...
May 2016: Pakistan Journal of Pharmaceutical Sciences
Jiang Zhu, Guangjie Duan, Hu Wang, Mianfu Cao, Yousheng Liu
Triggering receptor expressed on myeloid cells 1(TREM-1) is a newly identified member of the immunoglobulin superfamily and is extensively involved in the regulation of innate immunity. To determine the role of TREM-1 in innate antiviral immunity, we investigated TREM-1 expression and its downstream signaling effect in the murine bone marrow-derived macrophages or RAW264.7 macrophage-like mouse cell line by double-stranded RNA (dsRNA) stimulation. The level of TREM-1 expression was low at the baseline and could up-regulate markedly in dose- and time-dependent manners upon stimulation by dsRNA/poly IC...
July 1, 2016: Experimental Cell Research
Ming-Cheng Chang, Yu-Li Chen, Ying-Cheng Chiang, Ya-Jung Cheng, Yu-Wei Jen, Chi-An Chen, Wen-Fang Cheng, Wei-Zen Sun
The influence of morphine on host immunity and the underlying mechanism are still unclear. In the current study, we investigated the influence of morphine on dendritic cells (DCs), its possible mechanism of action, and the molecules that could reverse these effects. Morphine suppressed DC maturation, antigen presenting abilities, and the ability to activate antigen-specific CD8(+) T cells. Morphine-treated DCs also secreted higher concentrations of IL-10, but lower IL-6 and TNF-α. Morphine-treated DCs showed decreased ERK1/2 phosphorylation and reduced p38 dephosphorylation...
2016: American Journal of Cancer Research
R Singh, R Trickett, Cer Meyer, S Lewthwaite, D Ford
Introduction Acute gastrointestinal stress ulceration is a common and serious complication of trauma. Prophylactic proton pump inhibitors (PPIs) or histamine receptor antagonists have been used in poly-trauma, burns and head and spinal injuries, as well as on intensive care units, for the prevention of acute gastric stress ulcers. Methods We prospectively studied the use of prophylactic PPIs in with femoral neck fracture patients, gathering data on all acute gastric ulcer complications, including coffee-ground vomiting, malena and haematemesis...
July 2016: Annals of the Royal College of Surgeons of England
Mark J Lee, Alexander M Geller, Natalie C Bamford, Hong Liu, Fabrice N Gravelat, Brendan D Snarr, François Le Mauff, Joseé Chabot, Benjamin Ralph, Hanna Ostapska, Mélanie Lehoux, Robert P Cerone, Stephanie D Baptista, Evgeny Vinogradov, Jason E Stajich, Scott G Filler, P Lynne Howell, Donald C Sheppard
UNLABELLED: The mold Aspergillus fumigatus causes invasive infection in immunocompromised patients. Recently, galactosaminogalactan (GAG), an exopolysaccharide composed of galactose and N-acetylgalactosamine (GalNAc), was identified as a virulence factor required for biofilm formation. The molecular mechanisms underlying GAG biosynthesis and GAG-mediated biofilm formation were unknown. We identified a cluster of five coregulated genes that were dysregulated in GAG-deficient mutants and whose gene products share functional similarity with proteins that mediate the synthesis of the bacterial biofilm exopolysaccharide poly-(β1-6)-N-acetyl-D-glucosamine (PNAG)...
2016: MBio
Thomas Conrad, Anne-Susann Albrecht, Veronica Rodrigues de Melo Costa, Sascha Sauer, David Meierhofer, Ulf Andersson Ørom
Novel RNA-guided cellular functions are paralleled by an increasing number of RNA-binding proteins (RBPs). Here we present 'serial RNA interactome capture' (serIC), a multiple purification procedure of ultraviolet-crosslinked poly(A)-RNA-protein complexes that enables global RBP detection with high specificity. We apply serIC to the nuclei of proliferating K562 cells to obtain the first human nuclear RNA interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including few factors without known RNA-binding domains that are in good agreement with computationally predicted RNA binding...
2016: Nature Communications
Chandramohan Natarajan, Song-Yi Yao, Subramaniam Sriram
BACKGROUND: Impaired remyelination of demyelinated axons is a major cause of neurological disability. In inflammatory demyelinating disease of the central nervous system (CNS), although remyelination does happen, it is often incomplete, resulting in poor clinical recovery. Poly-IC a known TLR3 agonist and IL-33, a cytokine which is induced by poly-IC are known to influence recovery and promote repair in experimental models of CNS demyelination. METHODOLOGY AND PRINCIPAL FINDINGS: We examined the effect of addition of poly-IC and IL-33 on the differentiation and maturation of oligodendrocyte precursor cells (OPC) cultured in vitro...
2016: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"