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Federica Iavarone, Claudia Desiderio, Alberto Vitali, Irene Messana, Claudia Martelli, Massimo Castagnola, Tiziana Cabras
Proteomic surveys with top-down platforms are today revealing thousands of naturally occurring fragments of bigger proteins. Some of them have not functional meaning because they derive from pathways responsible for protein degradation, but many have specific functions, often completely different from that one of the parent proteins. These peptides encrypted in the protein sequence are nowadays called cryptides. They are frequent in the animal and plant kingdoms and represent a new interesting -omic field of investigation...
March 22, 2018: Critical Reviews in Biochemistry and Molecular Biology
Bohui Xu, Yan Xu, Gaoxing Su, Hongyan Zhu, Li Zong
Successful delivery of small interfering RNA (siRNA) into the cytoplasm of target cells relies on biocompatible and efficient vectors. In this study, a novel multifunctional core/shell nanoparticle [CS-SS-9R/BSA-c(RGDyK)] was developed to effectively deliver siVEGF to hepatocellular carcinoma cells (Bel-7402 cells). To improve the gene payload and transfection efficiency, a positively charged inner core (CS-SS-9R) was constructed by grafting nona-arginine (9R) onto chitosan (CS) using disulfide bonds. The negatively charged outer shell [BSA-c(RGDyK)] assembled on the surface of inner core by electrostatic forces that shielded high cationic charges and provided improved targeting...
March 22, 2018: Journal of Drug Targeting
Takaya Handa, Hiroko Kakita, Yu Tateishi, Tomomi Endo, Hiroyuki Suzuki, Toshiro Katayama, Tatsuo Tsukamoto, Eri Muso
BACKGROUND: IgA-dominant infection-related glomerulonephritis (IgA-IRGN) is a unique form of IRGN, which needs to be distinguished from IgA nephropathy (IgAN). METHODS: Thirteen patients with IgA-IRGN (IgA-IRGN group) and 122 with IgAN (IgAN group) were selected from 1788 patients who underwent kidney biopsy between 2000 and 2015 in Kitano Hospital. Data selected included clinical and serological parameters; light and electron microscope findings; immunofluorescence findings; and prognostic parameters like renal and overall survival and creatinine increase by > 50%...
March 21, 2018: Clinical and Experimental Nephrology
Tanveer A Wani, Ahmed H Bakheit, M A Abounassif, Seema Zargar
Binding of therapeutic agents to plasma proteins, particularly to serum albumin, provides valuable information in the drug development. This study was designed to evaluate the binding interaction of neratinib with bovine serum albumin (BSA). Neratinib blocks HER2 signaling and is effective in trastuzumab-resistant breast cancer treatment. Spectrofluorometric, UV spectrophotometric, and fourier transform infrared (FT-IR) and molecular docking experiments were performed to study this interaction. The fluorescence of BSA is attributed to the presence of tryptophan (Trp) residues...
2018: Frontiers in Chemistry
Takamasa Kitajima, Satoshi Marumo, Hiroshi Shima, Masahiro Shirata, Satoru Kawashima, Daiki Inoue, Yuko Katayama, Ryo Itotani, Minoru Sakuramoto, Motonari Fukui
Purpose: Episodic nocturnal hypercapnia (eNH) caused by rapid eye movement (REM) sleep-related hypoventilation is often noted in patients with advanced COPD. The purpose of this study was to clarify the clinical significance of eNH and the effectiveness of eNH-targeted noninvasive positive pressure ventilation (NPPV). Patients and methods: We enrolled patients with stable, severe, or very severe COPD with daytime arterial partial oxygen pressure PaO2 ≥55 mmHg and daytime arterial partial carbon dioxide pressure PaCO2 <55 mmHg, who underwent overnight transcutaneous carbon dioxide pressure (PtcCO2 ) monitoring from April 2013 to April 2016...
2018: International Journal of Chronic Obstructive Pulmonary Disease
Hong Zhou, HaiXin Qian
Objective: To investigate enteral nutrition's effect on serum inflammatory factors and the cardiac function of malnourished elderly patients with heart failure. Patients and methods: A total of 105 elderly patients with heart failure were randomly divided into 3 groups: Treatment Group A, Treatment Group B, and the Control Group (Group C), each group having 35 patients and being administered conventional heart failure treatment. Group A was treated with 500 mL·d-1 of enteral nutrition for 1 month...
2018: Clinical Interventions in Aging
Caitlin Trottier, Jeffrey Perl, Megan Freeman, Ravi Thadhani, Anders Berg, Sahir Kalim
Protein carbamylation is a post-translational urea-driven protein modification associated with mortality. Free amino acids (AAs) competitively inhibit protein carbamylation and parenteral AA therapy reduces carbamylation in hemodialysis (HD) patients. Peritoneal dialysis (PD) yields differences in urea clearance and AA balance compared with HD, but the influence of PD and intraperitoneal AA solutions on carbamylation is unclear. Thus, we first measured carbamylated albumin (C-Alb; a marker of carbamylation load) in 100 diabetic HD patients frequency-matched by age, sex, and race to 98 diabetic PD subjects from the IMPENDIA trial, which originally compared the metabolic effects of low-glucose PD solutions (incorporating icodextrin and AAs) to a control group (dextrose-only solutions)...
March 2018: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
Zhenlong Chen, Suellen D'Arc Oliveira, Adriana M Zimnicka, Ying Jiang, Tiffany Sharma, Stone Chen, Orly Lazarov, Marcelo G Bonini, Jacob M Haus, Richard D Minshall
We hypothesized that the maintenance of vascular homeostasis is critically dependent on the expression and reciprocal regulation of caveolin-1 (Cav-1) and endothelial nitric oxide synthase (eNOS) in endothelial cells (ECs). Skeletal muscle biopsies from subjects with type II diabetes showed 50% less Cav-1 and eNOS compared to lean healthy controls. The Cav-1:eNOS expression ratio was 200:1 in primary culture human ECs. Cav-1 siRNA reduced eNOS protein and gene expression in association with a 2-fold increase in eNOS phosphorylation and nitrate production per molecule of eNOS which was reversed in cells overexpressing Adv-Cav-1-GFP...
March 21, 2018: Molecular Biology of the Cell
Lu Zhao, Shanquan Chen, Xiaowei Shi, Hongcui Cao, Lanjuan Li
BACKGROUND: Liver disease is a major cause of death and disability. Mesenchymal stem cells (MSCs) show promise for the treatment of liver disease. However, whether MSC-based therapy is more effective than conventional treatment is unclear, as are the optimal MSC source, the administration frequency, and the most effective MSC delivery route. We therefore undertook a systematic review and meta-analysis of the therapeutic efficacy of MSCs against liver disease and the related factors. METHODS: We systematically searched Medline (PubMed), Cochrane Library, EMBASE, ClinicalTrials...
March 21, 2018: Stem Cell Research & Therapy
Dong Chi Zhou, Xiu Hong Yang, Xiao Li Zhan, Yan Hong Gu, Li Li Guo, Hui Min Jin
OBJECTIVE: This study aimed to evaluate the correlation between lean body mass (LBM) and nutritional status in hemodialysis (HD) patients to better predict their long-term prognosis. METHODS: Anthropometric body measurements and biochemical parameters were recorded from 222 patients on maintenance hemodialysis (MHD) at the Shanghai Pudong Hospital Hemodialysis Center. LBM was calculated using the serum creatinine index (LBM-SCR), mid-arm muscle circumference (LBM-MAMC), and dominant-arm hand-grip strength (LBM-HGS)...
March 1, 2018: International Journal of Artificial Organs
Ying Peng, Xueyuan Zhang, Yinci Zhu, Hui Wu, Shiyin Gu, Qingqing Chang, Yi Zhou, Guangji Wang, Jianguo Sun
Ib is a new nonpeptide AT1 receptor antagonist, which plays an active role in cardiovascular protection. Ib monoglucuronide has been identified as its main metabolite. A detailed study of Ib glucuronidation is important for predicting potential DDI. Besides, the elucidation of the "BSA effect" in Ib glucuronidation would make obtained kinetic parameters more predictive in IVIVE. "BSA effect" means that there is a significant change in in vitro kinetic parameters when generated from incubations performed in the presence of bovine serum albumin (BSA)...
March 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Kathrin Kettenbach, Laura M Reffert, Hanno Schieferstein, Stefanie Pektor, Raphael Eckert, Matthias Miederer, Frank Rösch, Tobias L Ross
Within the last decade, several folate-based radiopharmaceuticals for Single Photon Emission Computed Tomography (SPECT) and Positron Emission Tomography (PET) have been evaluated; however, there is still a lack of suitable18 F-folates for clinical PET imaging. Herein, we report the synthesis and evaluation of two novel18 F-folates employing strain-promoted and copper-catalyzed click chemistry. Furthermore, the influence of both click-methods on lipophilicity and pharmacokinetics of the18 F-folates was investigated...
March 17, 2018: Pharmaceuticals
Antonino Foti, Cristiano D'Andrea, Valentina Villari, Norberto Micali, Maria Grazia Donato, Barbara Fazio, Onofrio M Maragò, Raymond Gillibert, Marc Lamy de la Chapelle, Pietro G Gucciardi
Optical forces are used to aggregate plasmonic nanoparticles and create SERS-active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA-nanorod aggregations in a range from 100 µM to 50 nM by combining light scattering, plasmon resonance and SERS, and correlating the SERS signal with the concentration. Experimental data are fitted with a simple model describing the optical aggregation process...
March 17, 2018: Materials
Jiali Chen, Yan Li, Bing Yu, Daiwen Chen, Xiangbing Mao, Ping Zheng, Junqiu Luo, Jun He
Chlorogenic acid (CGA) is a natural phenolic acid, which is an important component of biologically active dietary phenols isolated from various species. Two experiments were conducted to investigate the effects of CGA on growth performance, antioxidant capacity, nutrient digestibility, diarrhea incidence, intestinal digestion and absorption function, and the expression levels of intestinal digestion and absorption-related genes in weaned pigs. In Exp. 1, 200 weaned pigs were randomly allotted to four dietary treatments and fed with a basal diet or a basal diet supplemented with 250, 500, or 1,000 mg/kg CGA, respectively, in a 14-d trial...
March 16, 2018: Journal of Animal Science
Chunyang Bao, Yueheng Yin, Qiang Zhang
Covalent coupling of hydrophobic polymers to the exterior of hydrophilic proteins would mediate unique macroscopic assembly of bioconjugates to generate amphiphilic superstructures as novel nanoreactors or biocompatible drug delivery system. The main objective of this study was to develop novel strategy for the synthesis of protein-polymer giant amphiphiles by the combination of copper mediated living radical polymerization and azide-alkyne cycloaddition reaction (CuAAC). Azide-functionalized succinimidyl ester was first synthesized for the facile introduction of azide group to proteins such as albumin from bovine serum (BSA) and laccase from Trametes versicolor...
March 21, 2018: Biomacromolecules
Raimundo Martins Gomes Junior, Lia Cavalcante Cezar, Gdayllon Cavalcante Meneses, Geraldo Bezerra da Silva Junior, José Huygenes Parente Garcia, Elizabeth De Francesco Daher
BACKGROUND: Acute kidney injury (AKI) is a common complication in the immediate postoperative period of patients undergoing liver transplantation. OBJECTIVE: The aim of this study was to evaluate preoperative risk factors for AKI after liver transplantation. METHODS: A cross-sectional study was conducted with adults submitted to orthotopic liver transplantation at a reference hospital in Fortaleza, Northeast of Brazil, from January to December 2016...
January 2018: Arquivos de Gastroenterologia
Rupert W Major, Mark R I Cheng, Robert A Grant, Saran Shantikumar, Gang Xu, Issaam Oozeerally, Nigel J Brunskill, Laura J Gray
BACKGROUND AND OBJECTIVES: Chronic kidney disease (CKD) is a global health burden and is independently associated with increased cardiovascular disease risk. Assessment of cardiovascular risk in the general population using prognostic models based on routinely collected risk factors is embedded in clinical practice. In CKD, prognostic models may misrepresent risk due to the interplay of traditional atherosclerotic and non-traditional risk factors. This systematic review's aim was to identify routinely collected risk factors for inclusion in a CKD-specific cardiovascular prognostic model...
2018: PloS One
Zhenhua Miao, Linda S Ertl, Dale Newland, Bin Zhao, Yu Wang, Xiaoping Zang, James J Campbell, Xiaoli Liu, Ton Dang, Shichang Miao, Antoni Krasinski, Sreenivas Punna, Yibin Zeng, Jeffrey McMahon, Penglie Zhang, Israel F Charo, Thomas J Schall, Rajinder Singh
Focal segmental glomerulosclerosis (FSGS) comprises a group of uncommon disorders that present with marked proteinuria, nephrotic syndrome, progressive renal failure and characteristic glomerular lesions on histopathology. The current standard of care for patients with FSGS include immunosuppressive drugs such as glucocorticoids followed by calcineurin inhibitors, if needed for intolerance or inadequate response to glucocorticoids. Renin-angiotensin-aldosterone (RAAS) blockers are also used to control proteinuria, an important signature of FSGS...
2018: PloS One
Bulei Sheng, Lotte Bach Larsen, Thao T Le, Di Zhao
α-Dicarbonyl compounds, which are widely generated during sugar fragmentation and oil oxidation, are important precursors of advanced glycation end products (AGEs). In this study, the effect of glycation derived from glyoxal (GO), methylglyoxal (MGO) and diacetyl (DA) on the in vitro digestibility of bovine serum albumin (BSA) was investigated. Glycation from α-dicarbonyl compounds reduced digestibility of BSA in both gastric and intestinal stage of digestion according to measurement of degree of hydrolysis...
March 21, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Marija Nišavić, Goran V Janjić, Amela Hozić, Marijana Petković, Miloš K Milčić, Zoran Vujčić, Mario Cindrić
Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4'-chloro-2,2':6',2''-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2'-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode...
March 21, 2018: Metallomics: Integrated Biometal Science
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