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Ju Song, Jian Huang, Zhi-Dong Li, Yue-Hong Long, Zhao-Bin Xing
The sweet taste and health effect of Lithocarpus polystachyus are mainly related flavonoid. To obtain Lithocarpus transcriptome database and flavonoid biosynthesis-related genes, the RNA-Seq techology (Illumina HiSeq 4000) was used to sequence its transcriptome. Six Gb database was assembled after assembly steps, and 41 043 of L. polystachyus unigenes were obtained. With blasting them with 7 data banks, all unigenes were involved in 51 GO-terms and 237 metabolic pathways. And furthermore 28 genes of the flavonoid biosynthesis-related were found...
February 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Gonçalo Espregueira Themudo, Helle Smidt Mogensen, Claus Børsting, Niels Morling
The HID-Ion AmpliSeq Ancestry Panel from Life Techologies includes 123 SNPs from the Seldin panel and 55 SNPs from Kidd panel in a single multiplex assay that helps to determine the continental biogeographic ancestry of individuals. We tested the panel on 104 Greenlanders, divided into a training set of 89 individuals and a test set of 15 individuals. All loci showed genotype distributions consistent with Hardy-Weinberg expectations. Linkage disequilibrium tests indicated that 14 pairs of loci were in association in Greenlanders...
September 2016: Forensic Science International. Genetics
Mehmet Selim Bilgin, Ali Erdem, Osman Sami Aglarci, Erhan Dilber
Two techological approaches for fabricating dentures; computer-aided design and computer-aided manufacturing (CAD/CAM) and rapid prototyping (RP), are combined with the conventional techniques of impression and jaw relation recording to determine their feasibility and applicability. Maxillary and mandibular edentulous jaw models were produced using silicone molds. After obtaining a gypsum working model, acrylic bases were crafted, and occlusal rims for each model were fabricated with previously determined standard vertical and centric relationships...
June 1, 2015: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
William Dauer
Isolated inherited dystonia-formerly referred to as primary dystonia-is characterized by abnormal motor functioning of a grossly normal appearing brain. The disease manifests as abnormal involuntary twisting movements. The absence of overt neuropathological lesions, while intriguing, has made it particularly difficult to unravel the pathogenesis of isolated inherited dystonia. The explosion of genetic techology enabling the identification of the causative gene mutations is transforming our understanding of dystonia pathogenesis, as the molecular, cellular and circuit level consequences of these mutations are identified in experimental systems...
October 2014: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
O F Kretenchuk, L P Alekseeva, O V Markina, G A Kozlova, M E Iagovkin, V D Kruglikov, I V Arkhangel'skaia
The article considers, the issue of producing the species-specific fluorescent monoclonal immunoglobulins to detect comma bacillus of serogrups O1 and O139 in the reaction of direct immunfluorescence. It is established that not only ascitic but culture fluid too can be the source of monoclonal antibodies for producing fluorescent conjugates. The optimal conditions are selected to produce the fluorescent monoclonal immunoglobulins-monoclonal antibodies. The corresponding producing techology can be reproduced at any time in view of availability of hybrid-producers of monoclonal antibodies O1 and monoclonal antibodies O139 in the institute cryodepositoty...
December 2012: Klinicheskaia Laboratornaia Diagnostika
Erik D Spoerke, Andrew K Boal, George D Bachand, Bruce C Bunker
Microtubules (MTs) and the MT-associated proteins (MAPs) are critical cooperative agents involved in complex nanoassembly processes in biological systems. These biological materials and processes serve as important inspiration in developing new strategies for the assembly of synthetic nanomaterials in emerging techologies. Here, we explore a dynamic biofabrication process, modeled after the form and function of natural aster-like MT assemblies such as centrosomes. Specifically, we exploit the cooperative assembly of MTs and MAPs to form artificial microtubule asters and demonstrate that (1) these three-dimensional biomimetic microtubule asters can be controllably, reversibly assembled and (2) they serve as unique, dynamic biotemplates for the organization of secondary nanomaterials...
March 26, 2013: ACS Nano
Y E M Koot, G Teklenburg, M S Salker, J J Brosens, N S Macklon
Despite expanding global experience with advanced reproductive technologies, the majority of IVF attempts do not result in a successful pregnancy, foremost as a result of implantation failure. The process of embryo implantation, a remarkably dynamic and precisely controlled molecular and cellular event, appears inefficient in humans and is poorly understood. However, insights gained from clinical implantation failure, early pregnancy loss, and emerging techologies that enable molecular interrogation of endometrial-embryo interactions are unravelling this major limiting step in human reproduction...
December 2012: Biochimica et Biophysica Acta
Zhuo-chun Huang, Yun-ying Shi, Bei Cai, Lan-lan Wang, Yong-kang Wu, Bin-wu Ying, Wei-hua Feng, Chao-jun Hu, Yong-zhe Li
OBJECTIVE: To establish a diagnostic model for systemic lupus erythematosus (SLE) using proteiomic fingerprint techology. METHODS: Blood samples were collected from 64 cases of SLE, 30 cases of rheumatoid arthritis (RA), 30 cases of Sjogren's syndrome (SS), 25 cases of systemic sclerosis (SSc), as well as 83 healthy controls. Proteomic spectra of these 232 serum samples were generated by proteomic fingerprint technology. Diagnostic model was established by a machine learning algorithm called decision boosting...
May 2009: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
J K Hulm, B T Matthias
This article deals with superconducting materials which have zero electrical resistance while carrying high electrical current densities (around 10(6) amperes per square centimeter) in high magnetic fields (up to 50 teslas). The technological importance of these materials is due to their use in the windings of loss-free electromagnets which generate high magnetic fields. Such magnets are the foundation for superconducting electrotechnology, a rapidly growing field whose applications include advanced electrical machines and fusion reactors...
May 23, 1980: Science
A B Kosolapov, E I Tsybul'ko, E V Makarova, E I Cherevach
Working out formula and techology of producing the syrup, which is based on herbs of Far Eastern Region (dog rose; leaves of currant, lime-tree; herbs St. John's wort, parsley. Nettle; licorice) and food dietary supplements--Laminal (the product of processing of brown seaweeds). Here are the results of the research work of chemical contents, energetic value, minor componential contents and microbiological values of syrup. The results of approbation of the syrup on the group of children (who catch a cold very often) showed the increase of coefficient of special immune lymphocytic-monocytical and cell-phagocytal protection...
2004: Voprosy Pitaniia
Richard Barry, Dimitri Ivanov
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. Microfluidics has been applied to DNA analysis methods and shown to accelerate DNA microarray assay hybridisation times...
March 31, 2004: Journal of Nanobiotechnology
R P Godio, O A Mercuri, F Forchiassin
Stereum hirsutum BAFC 2234 was tested for growth kinetics and ligninolytic enzyme production. The strain showed weak coincidences with widely studied organisms (Phanerochaete chrysosporium, Pleurotus ostreatus) when the response to nitrogen sources was assayed. This could be interpreted as a different regulation to nitrogen metabolism for S. hirsutum BAFC 2234. Alternative carbon sources and the addition of veratryl alcohol and wood extracts were also tested showing partial correspondence with other ligninolytic fungi...
January 2000: Revista Argentina de Microbiología
G C Carlon, R C Kahn, C Ray, W S Howland
A commercially available gas-chromatograph (Sentorr Gas Analyzer, Ohio Medical Products, Madison, WI) was tested, featuring continuous measurement of in vivo PaO2 and PaCO2 by means of a thin, heparin-coated catheter, inserted through an indwelling arterial line. Gas tensions are displayed every 4 min. The probes had a tendency to break rather easily, and a considerable proportion of them was faulty. We measured 105 paired determinations of blood gases obtained from patients in respiratory failure requiring mechanical ventilation with a Corning IL 175 Analyzer and displayed by the Sentorr Gas Analyzer...
July 1980: Critical Care Medicine
M Wada
No abstract text is available yet for this article.
December 1969: Rinsho Byori. the Japanese Journal of Clinical Pathology
B Castoldi, V Folisi, F Fontani, I Rocchi, G Sekules, A Zanotti-gerosa
No abstract text is available yet for this article.
January 1977: Bollettino Chimico Farmaceutico
J Arango, R Eisenmann
The current system of instruction in the School of Dentistry of the University of Panama is described. The object of the system is to prepare professional people capable of blending biological, techological, and social principles and applying them in different models of dental care for the solution of individual and collective oral health problems. The students take their clinical training in the metropolitan area (clinics on campus and in Santo Tomás Hospital) in the heavily populated rural area (the community hospital and primary schools), and in the sparsely populated countryside (where they work with teams of three auxiliaries in prevention programs for schoolchildren and programs of care for teachers and lend support to community activities)...
1978: Educación Médica y Salud
R Bressani
No abstract text is available yet for this article.
June 1979: Archivos Latinoamericanos de Nutrición
S A Schroeder
The model of comprehensive group practice advanced by the CCMC in 1932 has been an elusive reformist vision. Changes in the techniques and techology of medical practive will require new professional agendas as well a new political constituencies. Analysis of the process of these changes must balance avocacy of any uptopian forms of practice.
1978: Milbank Memorial Fund Quarterly. Health and Society
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