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Hypoxia reoxygenation

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https://www.readbyqxmd.com/read/28641008/hypoxia-induced-changes-in-the-fibroblast-secretome-exosome-and-whole-cell-proteome-using-cultured-cardiac-derived-cells-isolated-from-neonatal-mice
#1
Jake Cosme, Hongbo Guo, Sina Hadipour-Lakmehsari, Andrew Emili, Anthony O Gramolini
Cardiac fibroblasts (CF) represent a major subpopulation of cells in the developing and adult heart. Cardiomyocyte (CM) and CF intercellular communication occurs through paracrine interactions and modulate myocyte development and stress response. Detailed proteomic analysis of the CF secretome in normal and stressed conditions may offer insights into the role of CF in heart development and disease. Primary neonatal mouse CF were isolated and cultured for 24h in 21% (normoxic) or 2% (hypoxic) O2. Conditioned media was separated to obtain EXO and EXO-depleted secretome fractions...
June 22, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28636787/a-co-drug-conjugate-of-naringenin-and-lipoic-acid-mediates-neuroprotection-in-a-rat-model-of-oxidative-stress
#2
Tarek M Saleh, Monique C Saleh, Barry J Connell, Yang-Heon Song
Using our in vitro and in vivo models of oxidative stress, the current study was designed to determine the neuroprotective potential of naringenin, alone or in combination with lipoic acid. In our mixed neuronal culture exposed to hypoxia and subsequent reoxygenation, naringenin was shown to provide significant neuroprotection against cell death at a concentration of 2.5 μM. Lipoic acid (LA) did not produce neuroprotection at any concentration tested (0.25 to 100 μM). In contrast, when naringenin was covalently combined with LA, producing a novel compound named "VANL-100", significant neuroprotection was observed at a concentration as low as 2 x 10(-2) μM (100-fold more potent)...
June 21, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28632266/proteomic-analysis-reveals-xuesaitong-injection-attenuates-myocardial-ischemia-reperfusion-injury-by-elevating-pyruvate-dehydrogenase-mediated-aerobic-metabolism
#3
Xiaoping Zhao, Feng Zhang, Yi Wang
Xuesaitong injection (XST), which mainly consists of Panax notoginseng saponins, has been widely used for treating cardio-cerebral vascular diseases. However, the underlying mechanisms of XST associated with its cardioprotective effects are still unclear. To identify the potential target proteins of XST, two-dimensional gel electrophoresis (2-DE)-based proteomics was utilized to analyze the protein profile of myocardium in rats with myocardial ischemia/reperfusion (I/R) injury. The differentially expressed proteins were identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry...
June 20, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28629793/the-effects-of-hypoxia-on-active-ionic-transport-processes-in-the-gill-epithelium-of-hyperregulating-crab-carcinus-maneas
#4
Čedomil Lucu, Andreas Ziegler
Effects of hypoxia on the osmorespiratory functions of the posterior gills of the shore crab Carcinus maenas acclimated to 12ppt seawater (DSW) were studied. Short-circuit current (Isc) across the hemilamella (one epithelium layer supported by cuticle) was substantially reduced under exposure to 1.6, 2.0, or 2.5mg O2/L hypoxic saline (both sides of epithelium) and fully recovered after reoxygenation. Isc was reduced equally in the epithelium exposed to 1.6mg O2/L on both sides and when the apical side was oxygenated and the basolateral side solely exposed to hypoxia...
June 16, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28629357/xuefuzhuyu-decoction-protected-cardiomyocytes-against-hypoxia-reoxygenation-injury-by-inhibiting-autophagy
#5
Xiaowen Shi, Haiyan Zhu, Yuanyuan Zhang, Mingmei Zhou, Danli Tang, Huamin Zhang
BACKGROUND: XuefuZhuyu decoction (XFZY) is a well-known traditional Chinese herbal medicine for the treatment of various cardiovascular diseases, such as unstable angina pectoris and myocardial ischemia-reperfusion injury. However, the mechanism by which XFZY contributes to the amelioration of cardiac injury remains unclear. METHODS: H9C2 cells were cultured under the hypoxic condition for 10 h and reoxygenated for 2 h. In the presence of various concentrations of XFZY for 12 h, the cell viability was measured by MTT assay...
June 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28619106/bone-mesenchymal-stem-cells-ameliorate-ischemia-reperfusion-induced-damage-in-renal-epithelial-cells-via-microrna-223
#6
Xiaopeng Yuan, Xiaoping Wang, Chuanbao Chen, Jian Zhou, Ming Han
BACKGROUND: Recent studies have indicated that microRNA-223 (miR-223) plays a role in the tissue-protective effect of mesenchymal stem cells (MSCs). NLR family-pyrin domain containing 3 (NLRP3) was reported to affect a renal ischemia/reperfusion (I/R) injury by exerting a direct effect on the renal tubular epithelium. Therefore, we investigated how miR-223 and NLRP3 might function in kidneys exposed to conditions of ischemia and subsequent reperfusion. METHODS: Hypoxia/reoxygenation (H/R) murine renal tubular epithelial cells (RTECs) were cocultured with either MSCs or hypoxia-pretreated MSCs (htMSCs), after which the RTECs were examined for their viability and evidence of apoptosis...
June 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28615705/a-long-term-and-slow-releasing-hydrogen-sulfide-donor-protects-against-myocardial-ischemia-reperfusion-injury
#7
Xiaotian Sun, Wenshuo Wang, Jing Dai, Sheng Jin, Jiechun Huang, Changfa Guo, Chunsheng Wang, Liewen Pang, Yiqing Wang
Hydrogen sulfide (H2S) has been recognized as an important gasotransmitter exerting various physiological effects, especially in the cardiovascular system. Herein we investigated the cardioprotective effects of a novel long-term and slow-releasing H2S donor, DATS-MSN, using in vivo myocardial ischemia/reperfusion (I/R) models and in vitro hypoxia/reoxygenation cardiomyocyte models. Unlike the instant-releasing pattern of sodium hydrosulphide (NaHS), the release of H2S from DATS-MSN was quite slow and continuous both in the cell culture medium and in rat plasma (elevated H2S concentrations during 24 h and 72 h reperfusion)...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28614065/microrna-423-5p-facilitates-hypoxia-reoxygenation-induced-apoptosis-in-renal-proximal-tubular-epithelial-cells-by-targeting-gstm1-via-endoplasmic-reticulum-stress
#8
Xiao-Peng Yuan, Long-Shan Liu, Chuan-Bao Chen, Jian Zhou, Yi-Tao Zheng, Xiao-Ping Wang, Ming Han, Chang-Xi Wang
It has been reported that microRNAs (miRs) can regulate renal response to acute injury and members of them are believed to be important in maintenance of renal function and development of renal injury. We investigated the actions of microRNA-423-5p (miR-423-5p) and glutathione-S-transferase (GST) M1 after acute kidney injury. MiR-423-5p was up-regulated and GSTM1 was down-regulated in human kidney (HK-2) cells subjected to hypoxia/reoxygenation (H/R) and in rat kidneys subjected to ischemia/reperfusion (I/R) injury...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28606618/hypoxic-postconditioning-attenuates-apoptosis-via-activation-of-adenosine-a2a-receptors-on-dermal-microvascular-endothelial-cells-of-human-flaps
#9
Jiankun Cao, Wenzhi Li, Chao Cui, Binbin Li, Yi Xin, Lun Cai, Zhizhong Gong, Huang Lin
BACKGROUND: In recent years, many studies have demonstrated that endogenous adenosine induced by ischemia postconditioning reduces apoptosis in various models, but no study has clearly elucidated the effects of hypoxic postconditioning (HPC) in human dermal microvascular endothelial cells (HDMECs) of flaps, and the subtype of adenosine receptors involved remains unknown. In our study, we sought to identify the role of adenosine A2a receptor in the antiapoptotic effects of HPC. MATERIALS AND METHODS: The HDMECs were isolated from human upper eyelid tissue...
May 10, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28606475/coptisine-protects-cardiomyocyte-against-hypoxia-reoxygenation-induced-damage-via-inhibition-of-autophagy
#10
Yahui Wang, Qiangli Wang, Lu Zhang, Zunli Ke, Yuanyuan Zhao, Dongshan Wang, Hui Chen, Xi Jiang, Ming Gu, Shengjie Fan, Cheng Huang
Coptisine is a natural occurring isoquinoline alkaloid isolated from the traditional Chinese medicinal herb Rhizoma coptidis. Coptisine has been reported to have protective effects on reperfusion injury in cardiomyocytes, however, the underlying mechanism remains uncertain. Here, we used a hypoxia/reoxygenation (H/R)-treated H9c2 cell model to study the protective effects of coptisine on cardiomyocyte. The results showed that NaS2O4 induced hypoxia/reoxygenation model increased apoptosis and up-regulated autophagy marker LC3-II and cleaved Caspase-3, Beclin1 and Sirt1 levels...
June 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28598489/nicotinic-acetylcholine-receptor-mediated-protection-of-the-rat-heart-exposed-to-ischemia-reperfusion
#11
Spyros A Mavropoulos, Nayaab S Khan, Asaph C J Levy, Bradley T Faliks, Cristina P Sison, Valentin A Pavlov, Youhua Zhang, Kaie Ojamaa
Reperfusion injury following acute myocardial infarction is associated with significant morbidity. Activation of neuronal or non-neuronal cholinergic pathways in the heart has been shown to reduce ischemic injury and this effect has been attributed primarily to muscarinic acetylcholine receptors. In contrast, the role of nicotinic receptors, specifically alpha-7 subtype (α7nAChR) in the myocardium remains unknown which offers an opportunity to potentially repurpose several agonists/modulators that are currently under development for neurologic indications...
June 8, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28590356/plasma-succinate-is-a-predictor-of-mortality-in-critically-injured-patients
#12
Angelo D'Alessandro, Hunter B Moore, Ernest E Moore, Julie A Reisz, Matthew J Wither, Arsen Ghasabyan, James Chandler, Christopher C Silliman, Kirk C Hansen, Anirban Banerjee
BACKGROUND: Trauma is the leading cause of mortality under the age of 40. Recent observations on metabolic reprogramming during hypoxia and ischemia indicate that hypoxic mitochondrial uncoupling promotes the generation of succinate, which in turn mediates reperfusion injury and inflammatory sequelae upon reoxygenation. Plasma levels of succinate significantly increase in response to trauma and hemorrhage in experimental models and clinical samples, suggesting that succinate may represent a candidate marker of systemic perfusion in trauma...
June 6, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28589995/trans-polydatin-protects-the-mouse-heart-against-ischemia-reperfusion-injury-via-inhibition-of-the-renin-angiotensin-system-ras-and-rho-kinase-rock-activity
#13
Dong Ming, Liao Songyan, Chen Yawen, Zheng Na, Ma Jing, Xiao Zhaowen, Liu Ye, Ding Wa, Liu Jie
BACKGROUND: Recent studies highlighted the protective benefits of a Chinese herb extract from polygonum cuspidatum, trans-polydatin, on cardiac disease. We investigated the therapeutic effect of trans-polydatin on myocardial ischemia/reperfusion (IR) injury and the underlying mechanisms related to the renin-angiotensin system (RAS) and RhoA kinase (ROCK) pathway. METHODS AND RESULTS: Experiments were performed on neonatal rats' ventricular myocytes that were subjected to hypoxia-reoxygenation (simulated IR, SIR) and on adult mice which were subjected to left anterior descending coronary artery occlusion for 45 min followed by a one-week reperfusion...
June 7, 2017: Food & Function
https://www.readbyqxmd.com/read/28582278/exosomes-from-adipose-derived-mesenchymal-stem-cells-protect-ischemic-myocardium-from-ischemia-reperfusion-injury-via-wnt-%C3%AE-catenin-signaling-pathway
#14
Xiaojun Cui, Zhangyou He, Zihao Liang, Zhenyi Chen, Haifeng Wang, Jiankai Zhang
Mesenchymal stem cells (MSCs) and their secreted exosomes exert a cardioprotective role in jeopardized myocardium. However, the specific effects and underlying mechanisms of exosomes derived from adipose-derived MSCs (ADMSCs) on myocardial ischemia/reperfusion (I/R) injury remain largely unclear. In this study, ADMSC-derived exosomes (ADMSCs-ex) were administrated into the rats subjected to I/R injury and H9c2 cells exposed to Hypoxia/reoxygenation (H/R). Consequently, administration of ADMSCs-ex significantly reduced I/R-induced myocardial infarction, accompanied with a decrease in serum levels of creatine kinase-myocardial band (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin I (cTnI)...
June 5, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28581448/trimetazidine-protects-cardiomyocytes-against-hypoxia-reoxygenation-injury-by-promoting-amp-activated-protein-kinase-dependent-autophagic-flux
#15
Yongkang Zhong, Peiyao Zhong, Shangfei He, Ya Zhang, Lu Tang, Yuanna Ling, Shanshan Fu, Ying Tang, Pingzhen Yang, Tao Luo, Baihe Chen, Aihua Chen, Xianbao Wang
Trimetazidine (TMZ), a metabolic agent, may protect against myocardial ischemia/reperfusion injury. Because of the critical role of autophagy in cardioprotection, we aimed to evaluate whether autophagy was involved in TMZ-induced protection during hypoxia/reoxygenation (H/R). Neonatal rat cardiomyocytes were subjected to H/R injury, and they were divided into 7 groups: control, control+TMZ, control+chloroquine (Cq)/compound C (com C), H/R, H/R+TMZ, H/R+Cq/com C, and H/R+TMZ+Cq/com C. Autophagic flux was primarily assessed by Western blot and tandem fluorescent mRFP-GFP-LC3...
June 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28579900/imperatorin-protects-h9c2-cardiomyoblasts-cells-from-hypoxia-reoxygenation-induced-injury-through-activation-of-erk-signaling-pathway
#16
Bihong Liao, Ruimian Chen, Feng Lin, Aihuan Mai, Jie Chen, Huimin Li, Shaohong Dong, Zhenglei Xu
BACKGROUND: Imperatorin is a compound found in plants and has been widely used in Chinese medicine for many years. It has many pharmacological effects, including the recently reported anti-apoptotic function, however, the mechanism largely remains unclear. This study is aimed to elucidate the mechanism of Imperatorin's anti-apoptotic function. METHODS: A model of hypoxia and reoxygenation (H/R) treated h9c2 cardiomyoblasts was successfully constructed. The cells were treated with H/R condition, and followed by adding Imperatorin alone, Imperatorin with ERK inhibitor and/or ERK inhibitor alone, to examine the cell viability by Cell Counting Kit-8 assay, cell apoptosis rate by flow cytometry, and ERK expression by Western-blot under different conditions...
May 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28579187/functional-mri-bold-response-in-sickle-mice-with-hyperalgesia
#17
Ying Wang, Xiao Wang, Wei Chen, Kalpna Gupta, Xiao-Hong Zhu
Patients with sickle cell anemia (SCA) have abnormal hemoglobin (sickle hemoglobin S) leading to the crystallization of hemoglobin chains in red blood cells (RBCs), which assume sickle shape and display reduced flexibility. Sickle RBCs (sRBCs) adhere to vessel walls and block blood flow, thus preventing oxygen delivery to the tissues leading to vaso-occlusive crises (VOC), acute pain and organ damage. SCA patients often have chronic pain that can be attributed to inflammation, vasculopathy, neuropathy, ischemia-reperfusion injury and organ damage...
June 2017: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/28578351/protective-effects-of-microrna-126-on-human-cardiac-microvascular-endothelial-cells-against-hypoxia-reoxygenation-induced-injury-and-inflammatory-response-by-activating-pi3k-akt-enos-signaling-pathway
#18
Hong-Hui Yang, Yan Chen, Chuan-Yu Gao, Zhen-Tian Cui, Jian-Min Yao
OBJECTIVE: This study explored the protective effects of the microRNA-126 (miR-126)-mediated PI3K/Akt/eNOS signaling pathway on human cardiac microvascular endothelial cells (HCMECs) against hypoxia/reoxygenation (H/R)-induced injury and the inflammatory response. METHODS: Untreated HCMECs were selected for the control group. After H/R treatment and cell transfection, the HCMECs were assigned to the H/R, miR-126 mimic, mimic-negative control (NC), miR-126 inhibitor, inhibitor-NC, wortmannin (an inhibitor of PI3K) and miR-126 mimic + wortmannin groups...
June 5, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28569786/brg1-mediated-nrf2-ho-1-pathway-activation-alleviates-hepatic-ischemia-reperfusion-injury
#19
Mian Ge, Weifeng Yao, Dongdong Yuan, Shaoli Zhou, Xi Chen, Yihan Zhang, Haobo Li, Zhengyuan Xia, Ziqing Hei
Cytoprotective gene heme oxygenase 1 (HO-1) could be induced by nuclear factor E2-related factor 2 (Nrf2) nuclear translocation. The purpose of this study was to determine the role of Brahma-related gene 1 (Brg1), a catalytic subunit of SWI2/SNF2-like chromatin remodeling complexes, in Nrf2/HO-1 pathway activation during hepatic ischemia-reperfusion (HIR). Our results showed that hepatic Brg1 was inhibited during early HIR while Brg1 overexpression reduced oxidative injury in CMV-Brg1 mice subjected to HIR...
June 1, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28562339/fam3a-mediates-ppar%C3%AE-s-protection-in-liver-ischemia-reperfusion-injury-by-activating-akt-survival-pathway-and-repressing-inflammation-and-oxidative-stress
#20
Zhenzhen Chen, Junpei Wang, Weili Yang, Ji Chen, Yuhong Meng, Bin Geng, Qinghua Cui, Jichun Yang
FAM3A is a novel mitochondrial protein, and its biological function remains largely unknown. This study determined the role and mechanism of FAM3A in liver ischemia-reperfusion injury (IRI). In mouse liver after IRI, FAM3A expression was increased. FAM3A-deficient mice exhibited exaggerated liver damage with increased serum levels of AST, ALT, MPO, MDA and oxidative stress when compared with WT mice after liver IRI. FAM3A-deficient mouse livers had a decrease in ATP content, Akt activity and anti-apoptotic protein expression with an increase in apoptotic protein expression, inflammation and oxidative stress when compared WT mouse livers after IRI...
May 11, 2017: Oncotarget
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