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Hypoxia reoxygenation

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https://www.readbyqxmd.com/read/28106131/hif-1%C3%AE-induction-during-reperfusion-avoids-maladaptive-repair-after-renal-ischemia-reperfusion-involving-mir127-3p
#1
Elisa Conde, Sara Giménez-Moyano, Laura Martín-Gómez, Macarena Rodríguez, M Edurne Ramos, Elia Aguado-Fraile, Ignacio Blanco-Sanchez, Ana Saiz, María Laura García-Bermejo
Ischemia/reperfusion (I/R) leads to Acute Kidney Injury. HIF-1α is a key factor during organ response to I/R. We previously demonstrated that HIF-1α is induced during renal reperfusion, after ischemia. Here we investigate the role of HIF-1α and the HIF-1α dependent mechanisms in renal repair after ischemia. By interference of HIF-1α in a rat model of renal I/R, we observed loss of expression and mis-localization of e-cadherin and induction of α-SMA, MMP-13, TGFβ, and collagen I. Moreover, we demonstrate that HIF-1α inhibition promotes renal cell infiltrates by inducing IL-1β, TNF-α, MCP-1 and VCAM-1, through NFkB activity...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28104397/pink1-alleviates-myocardial-hypoxia-reoxygenation-injury-by-ameliorating-mitochondrial-dysfunction
#2
Yang Li, Liangxian Qiu, Xiping Liu, Zhiwen Hou, Bo Yu
PTEN inducible kinase-1 (PINK1) mutant induces mitochondrial dysfunction of cells, resulting in an inherited form of Parkinson's disease. However its exact role in the cardiomyocytes is unclear. The present study examined the function of PINK1 in hypoxia-reoxygenation (H/R) induced H9c2 cell damage and its potential mechanism. The H/R model in H9c2 cells was established by 6 h of hypoxia and 12 h of reoxygenation. The CCK8 and LDH assay indicated that the cell viability was obviously reduced after H/R. The expression of PINK1 was decreased in H/R-induced H9c2 cells compared with control group...
January 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28100347/-salvianolate-protects-h9c2-cells-from-hypoxia-reoxygenation-injury-induced-apoptosis-by-attenuating-mitochondrial-dna-oxidative-damage
#3
R C Yue, X L Yang, R Y Zhang, S Liu, J Liu, J Zeng, H Liang, W Wang, H X Hu, C Y Zeng
Objective: To investigate the possible mechanism related to the protective effects of salvianolate in H9c2 cells underwent hypoxia/reoxygenation (H/R)-injury. Methods: H9c2 cells were divided into four groups: control group, salvianolate group (S group), H/R group, and salvianolate+ H/R group(S+ H/R group), in which the H9c2 cells were pretreated with salvianolate before H/R-treatment.Apoptotic cells were detected by Tunel assays and AnnexinⅤ-FITC apoptosis detection kit.The intracellular ATP level, the change of mitochondrial membrane potential and the mitochondrial DNA oxidative damage were also determined in these groups...
January 25, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28096195/the-multifunctional-mitochondrial-epac1-controls-myocardial-cell-death
#4
Loubina Fazal, Marion Laudette, Sílvia Paula-Gomes, Sandrine Pons, Caroline Conte, Florence Tortosa, Pierre Sicard, Yannis Sainte-Marie, Malik Bisserier, Olivier Lairez, Alexandre Lucas, Jérôme Roy, Bijan Ghaleh, Jeremy Fauconnier, Jeanne Mialet-Perez, Frank Lezoualc'h
RATIONALE: Although the second messenger cyclic AMP (cAMP) is physiologically beneficial in the heart, it largely contributes to cardiac disease progression when dysregulated. Current evidence suggests that cAMP is produced within mitochondria. However, mitochondrial cAMP signaling and its involvement in cardiac pathophysiology are far from being understood. OBJECTIVE: To investigate the role of mitochondrial exchange protein directly activated by cAMP 1 (MitEpac1) in ischemia/reperfusion (I/R) injury...
January 17, 2017: Circulation Research
https://www.readbyqxmd.com/read/28095922/long-noncoding-rna-braveheart-promotes-cardiogenic-differentiation-of-mesenchymal-stem-cells-in-vitro
#5
Jingying Hou, Huibao Long, Changqing Zhou, Shaoxin Zheng, Hao Wu, Tianzhu Guo, Quanhua Wu, Tingting Zhong, Tong Wang
BACKGROUND: Mesenchymal stem cells (MSCs) have limited potential of cardiogenic differentiation. In this study, we investigated the influence of long noncoding RNA Braveheart (lncRNA-Bvht) on cardiogenic differentiation of MSCs in vitro. METHODS: MSCs were obtained from C57BL/6 mice and cultured in vitro. Cells were divided into three groups: blank control, null vector control, and lncRNA-Bvht. All three groups experienced exposure to hypoxia (1% O2) and serum deprivation for 24 h, and 24 h of reoxygenation (20% O2)...
January 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28079888/antiphospholipid-antibodies-enhance-rat-neonatal-cardiomyocyte-apoptosis-in-an-in-vitro-hypoxia-reoxygenation-injury-model-via-p38-mapk
#6
Lauren T Bourke, Thomas McDonnell, James McCormick, Charis Pericleous, Vera M Ripoll, Ian Giles, Anisur Rahman, Anastasis Stephanou, Yiannis Ioannou
A significant amount of myocardial damage during a myocardial infarction (MI) occurs during the reperfusion stage, termed ischaemia/reperfusion (I/R) injury, and accounts for up to 50% of total infarcted tissue post-MI. During the reperfusion phase, a complex interplay of multiple pathways and mechanisms is activated, which ultimately leads to cell death, primarily through apoptosis. There is some evidence from a lupus mouse model that lupus IgG, specifically the antiphospholipid (aPL) antibody subset, is pathogenic in mesenteric I/R injury...
January 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28073079/trail-facilitates-cytokine-expression-and-macrophage-migration-during-hypoxia-reoxygenation-via-er-stress-dependent-nf-%C3%AE%C2%BAb-pathway
#7
Yinan Jiang, Xiaoyan Chen, Mengya Fan, Hui Li, Weina Zhu, Xi Chen, Chenghua Cao, Rui Xu, Yaohui Wang, Yuanfang Ma
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is known as a key molecule to induce cancer cell apoptosis, has also been found to participate in the process of ischemia/reperfusion (I/R) injury. Infiltrated macrophages play dual roles in inflammatory injury and healing following I/R. Whether TRAIL has any effect on macrophages during this process remains elusive. Here we showed that I/R triggered the expressions of TRAIL, DR5 and cytokines (IL-1β, TNFα, CCL-2 and ICAM-1), in addition to macrophage infiltration, which could be abolished by TRAIL neutralizing antibody...
January 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28056323/-the-role-of-neuroglobin-in-oxygen-glucose-deprivation-and-reoxygenation-induced-mitochondrial-depolarization-and-reactive-oxygen-species-production-in-sh-sy5y-cells
#8
S Y Deng, Y H Ai, L N Zhang, L Wu, C X Chen, Y M Wang, Z Y Liu, L Huang, Q Y Peng
Objective: To investigate the role of neuroglobin (NGB) in oxygen-glucose deprivation and reoxygenation (OGD/R) induced mitochondrial depolarization and reactive oxygen species (ROS)production in a human neuroblastoma cell line (SH-SY5Y). Methods: SH-SY5Y cells were transfected with lentivirus to establish a stable cell line of NGB knockdown (KD). After treated with OGD/R, cells were collected at different time points to analyze NGB mRNA and protein levels. Furthermore, cells were stained with JC-1 and DCFH-DA to evaluate mitochondrial depolarization and ROS production by inverted fluorescence microscope...
January 1, 2017: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/28046118/transcriptome-analysis-identifies-key-metabolic-changes-in-the-hooded-seal-cystophora-cristata-brain-in-response-to-hypoxia-and-reoxygenation
#9
Mariana Leivas Müller Hoff, Andrej Fabrizius, Nicole U Czech-Damal, Lars P Folkow, Thorsten Burmester
The brain of diving mammals tolerates low oxygen conditions better than the brain of most terrestrial mammals. Previously, it has been demonstrated that the neurons in brain slices of the hooded seal (Cystophora cristata) withstand hypoxia longer than those of mouse, and also tolerate reduced glucose supply and high lactate concentrations. This tolerance appears to be accompanied by a shift in the oxidative energy metabolism to the astrocytes in the seal while in terrestrial mammals the aerobic energy production mainly takes place in neurons...
2017: PloS One
https://www.readbyqxmd.com/read/28036282/sulodexide-pretreatment-attenuates-renal-ischemia-reperfusion-injury-in-rats
#10
Jianyong Yin, Weibin Chen, Fenfen Ma, Zeyuan Lu, Rui Wu, Guangyuan Zhang, Niansong Wang, Feng Wang
Sulodexide is a potent antithrombin agent, however, whether it has beneficial effects on renal ischemia-reperfusion injury (IRI) remains unknown. In the present study, we assessed the therapeutic effects of sulodexide in renal IRI and tried to investigate the potential mechanism. One dose of sulodexide was injected intravenously in Sprague-Dawley rats 30 min before bilateral kidney ischemia for 45 min. The animals were sacrificed at 3h and 24h respectively. Our results showed that sulodexide pretreatment improved renal dysfunction and alleviated tubular pathological injury at 24h after reperfusion, which was accompanied with inhibition of oxidative stress, inflammation and cell apoptosis...
December 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/28033580/extracellular-cl-free-induced-cardioprotection-against-hypoxia-reoxygenation-is-associated-with-attenuation-of-mitochondrial-permeability-transition-pore
#11
Xian-Gui Zhang, Le Zhao, Yi Zhang, Yuan-Yuan Li, Huan Wang, Guang-Ling Duan, Lin Xiao, Xiao-Ran Li, He-Ping Chen
The isotonic substitution of extracellular chloride by gluconate (extracellular Cl(-)-free) has been demonstrated to elicit cardioprotection by attenuating ischaemia/reperfusion-induced elevation of intracellular chloride ion concentration ([Cl(-)]i). However, the downstream mechanism underlying the cardioprotective effect of extracellular Cl(-)-free is not fully established. Here, it was investigated whether extracellular Cl(-)-free attenuates mitochondrial dysfunction after hypoxia/reoxygenation (H/R) and whether mitochondrial permeability transition pore (mPTP) plays a key role in the extracellular Cl(-)-free cardioprotection...
December 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28033113/high-brachytherapy-doses-can-counteract-hypoxia-in-cervical-cancer-a-modelling-study
#12
Emely Lindblom, Alexandru Dasu, Catharina Beskow, Iuliana Toma-Dasu
Tumour hypoxia is a well-known adverse factor for the outcome of radiotherapy. For cervical tumours in particular, several studies indicate large variability in tumour oxygenation. However, clinical evidence shows that the management of cervical cancer including brachytherapy leads to high rate of success. It was the purpose of this study to investigate whether the success of brachytherapy for cervical cancer, seemingly regardless of oxygenation status, could be explained by the characteristics of the brachytherapy dose distributions...
December 29, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28030900/tumor-hypoxia-and-reoxygenation-the-yin-and-yang-for-radiotherapy
#13
REVIEW
Beom-Ju Hong, Jeongwoo Kim, Hoibin Jeong, Seoyeon Bok, Young-Eun Kim, G-One Ahn
Tumor hypoxia, a common feature occurring in nearly all human solid tumors is a major contributing factor for failures of anticancer therapies. Because ionizing radiation depends heavily on the presence of molecular oxygen to produce cytotoxic effect, the negative impact of tumor hypoxia had long been recognized. In this review, we will highlight some of the past attempts to overcome tumor hypoxia including hypoxic radiosensitizers and hypoxia-selective cytotoxin. Although they were (still are) a very clever idea, they lacked clinical efficacy largely because of 'reoxygenation' phenomenon occurring in the conventional low dose hyperfractionation radiotherapy prevented proper activation of these compounds...
December 2016: Radiation Oncology Journal
https://www.readbyqxmd.com/read/28025989/microrna-142-3p-inhibits-hypoxia-reoxygenation%C3%A2-induced-apoptosis-and-fibrosis-of-cardiomyocytes-by-targeting-high-mobility-group-box%C3%A2-1
#14
Yi Wang, Min Ouyang, Qiong Wang, Zaijin Jian
Myocardial ischemia/reperfusion (I/R) injury may cause the apoptosis of cardiomyocytes as well as cardiac fibrosis, which is characterized as the transdifferentiation of fibroblasts to myofibroblasts and collagen deposition. MicroRNAs (miRNAs or miRs) have been demonstrated to be involved in myocardial I/R injury. However, the underlying molecular mechanism remains largely unclear. In the present study, mouse cardiomyocyte M6200 cells were treated with hypoxia/reoxygenation (H/R). Our data indicated that H/R treatment led to cell apoptosis, the increased expression of fibrosis‑related proteins, namely collagen I, II, III, and fibronectin, as well as the downregulation of miR-142-3p in M6200 cells...
November 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28009855/tetramethyl-pyrazine-protects-hippocampal-neurons-against-anoxia-reoxygenation-injury-through-inhibiting-apoptosis-mediated-by-jnk-mark-signal-pathway
#15
Ming Zhong, Wuhua Ma, Xiong Zhang, Yong Wang, Xiaoqiu Gao
BACKGROUND Tetramethyl pyrazine (TMP) is a typical biologically active alkaloid isolated from the Chinese herb Ligusticum walliichi. It has been reported that TMP shows neuroprotective and stroke injury reductive properties in cerebral ischemia/reperfusion (I/R) animal models. In the present study we sought to investigate the effect and potential intervention mechanism of TMP in anoxia/reoxygenation (A/R) rat hippocampal neurons. MATERIAL AND METHODS After being cultured for 7 days, primary hippocampal neurons were randomly assigned into a normal control group (N), a TMP group (C: 0 ug/ml, L: 60 ug/ml, M: 200ug/ml and H: 800 ug/ml), and a JNK inhibitor group (S: SP600125, 10 μmol/L)...
December 23, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28006785/mir-155-is-involved-in-renal-ischemia-reperfusion-injury-via-direct-targeting-of-foxo3a-and-regulating-renal-tubular-cell-pyroptosis
#16
Haoyu Wu, Tao Huang, Liang Ying, Conghui Han, Dawei Li, Yao Xu, Ming Zhang, Shan Mou, Zhen Dong
BACKGROUND/AIMS: Ischemia/reperfusion injury (IRI) plays a crucial role in renal transplantation and can cause renal failure associated with pyroptosis, a pro-inflammatory-induced programmed cell death. Small endogenous non-coding RNAs have been shown to be involved in renal ischemia/reperfusion injury. This study was performed to investigate which miRNAs regulate pyroptosis in response to renal ischemia/reperfusion injury and determine the mechanism underlying this regulation. METHODS: An in vivo rat model of renal IRI was established, and the serum and kidneys were harvested 24 h after reperfusion to assess renal function and histological changes...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27993557/inhibition-of-endoplasmic-reticulum-stress-by-neuregulin-1-protects-against-myocardial-ischemia-reperfusion-injury
#17
Shan-Juan Fang, Peng-Yang Li, Chun-Mei Wang, Yi Xin, Wei-Wei Lu, Xiao-Xia Zhang, Song Zuo, Chang-Sheng Ma, Chao-Shu Tang, Shao-Ping Nie, Yong-Fen Qi
Neuregulin-1 (NRG-1), an endogenously produced polypeptide, is the ligand of cardiomyocyte ErbB receptors, with cardiovascular protective effects. In the present study, we explored whether the cardioprotective effect of NRG-1 against I/R injury is mediated by inhibiting myocardial endoplasmic reticulum (ER) stress. In vitro, NRG-1 directly inhibited the upregulation of ER stress markers such as glucose-regulated protein 78, CCAAT/enhancer binding protein homologous protein and cleaved caspase-12 induced by the ER stress inducers tunicamycin or dithiothreitol in both neonatal and adult ventricular myocytes...
December 16, 2016: Peptides
https://www.readbyqxmd.com/read/27988284/anti-effects-of-cordycepin-to-hypoxia-induced-membrane-depolarization-on-hippocampal-ca1-pyramidal-neuron
#18
Chong Chen, Xin-Ping Liu, Wei Jiang, Bin Zeng, Wei Meng, Li-Ping Huang, Yu-Ping Li, Wei Sun, Chun-Hua Yuan, Li-Hua Yao
Cordycepin has important neuroprotective effects in hypoxic or ischemic insult. However, the direct electrophysiological evidence of cordycepin's neuroprotection from hypoxic or ischemic insult remains unknown. Hence, in this study, the electrophysiological mechanism by which cordycepin protects against ischemic and hypoxic damages has been studied using an energy-deprivation injury model through whole-cell patch clamp techniques. Results revealed that cordycepin (80µM) significantly delayed hypoxia-induced membrane depolarization, including cordycepin reduced slope, and extended the duration of slow depolarization, prolonged the ability to generate spontaneous action potential (AP) firing, delayed the onset of rapid depolarization, and maintained the more hyperpolarized membrane potential after rapid depolarization...
December 15, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27982695/17-dmag-protects-against-hypoxia-reoxygenation-induced-cell-injury-in-ht22-cells-through-akt-nrf2-ho-1-pathway
#19
Zhuopeng Jia, Arui Dong, Hongmin Che, Yu Zhang
Oxidative stress is well known to play a pivotal role in hypoxia/reoxygenation (H/R)-induced neuron injury. On the basis of this fact, antioxidative agents have been demonstrated to be neuroprotective. 17-DMAG (HSP90 inhibitor) is reported to have neuroprotective effects in vitro, which may interfere with oxidative stress through reduction in pro-oxidative factors. However, little is known about its effects on H/R-induced neuron injury and the underlying mechanisms. In this study, the effects of 17-DMAG on H/R-treated HT22 cells were investigated...
December 16, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27974731/egb-761-protects-cardiac-microvascular-endothelial-cells-against-hypoxia-reoxygenation-injury-and-exerts-inhibitory-effect-on-atm-pathway
#20
Chao Zhang, Deng-Feng Wang, Zhuang Zhang, Dong Han, Kan Yang
Ginkgo biloba extract (EGb 761) has been widely clinically used to reduce myocardial ischemia reperfusion injury (MIRI). Microvascular endothelial cells (MVECs) may be a proper cellular model in vitro for the effect and mechanism study against MIRI. However, the effect of EGb 761 on MVECs resisting hypoxia/reoxygenation (H/R) injury is little reported. In this study, H/R-injuried MVECs were treated with EGb 761, then cell viability, apoptosis, ROS production, SOD activity, caspase-3 activity, and the protein level of ATM, γ-H2AX, p53, Bax were measured...
December 14, 2016: Journal of Microbiology and Biotechnology
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