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Hypoxia reoxygenation

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https://www.readbyqxmd.com/read/28726782/pkc%C3%AE-phosphorylates-traf2-to-protect-against-intestinal-ischemia-reperfusion-induced-injury
#1
Wei Zhou, Jihong Yao, Guangzhi Wang, Zhao Chen, Zhenlu Li, Dongcheng Feng, Yang Li, Wasim Qasim, Wenzhi Tan, Shili Ning, Xiaofeng Tian
Intestinal ischemia-reperfusion (I/R) is a common clinical problem that occurs during various clinical pathological processes. Excessive apoptosis has an indispensable role in intestinal I/R injury. Tumor necrosis factor receptor-associated factor 2 (TRAF2) and PKCζ have an essential role in apoptosis. Here, we aimed to investigate the effects of PKCζ and TRAF2 and to explore the correlation between PKCζ and TRAF2 in intestinal I/R injury. Mice were subjected to intestinal I/R injury in vivo. In vitro experiments were conducted by treating Caco-2 cells with hypoxia/reoxygenation (H/R) stimulation to simulate intestinal I/R...
July 20, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28724979/impairment-of-coronary-endothelial-function-by-hypoxia-reoxygenation-involves-trpc3-inhibition-mediated-kca-channel-dysfunction-implication-in-ischemia-reperfusion-injury
#2
Xiang-Chong Wang, Wen-Tao Sun, Jie Fu, Jun-Hao Huang, Cheuk-Man Yu, Malcolm John Underwood, Guo-Wei He, Qin Yang
Despite increasing knowledge of the significance of calcium-activated potassium (KCa) and canonical transient receptor potential (TRPC) channels in endothelial physiology, no studies so far have investigated the link between these two distinct types of channels in the control of vascular tone in pathological conditions. We previously demonstrated that hypoxia-reoxygenation (H-R) inhibits endothelial KCa and TRPC3 channels in porcine coronary arteries (PCAs). The present study further investigated whether modulation of TRPC3 is involved in H-R-induced KCa channel inhibition and associated vasodilatory dysfunction using approaches of wire myography, whole-cell voltage-clamp, and coimmunoprecipitation...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720379/inhibition-of-12-15-lox-ameliorates-cognitive-and-cholinergic-dysfunction-in-mouse-model-of-hypobaric-hypoxia-via-attenuation-of-oxidative-nitrosative-stress
#3
Richa Choudhary, Udayabanu Malairaman, Anju Katyal
12/15 Lipoxygenase has recently been described as potent propagator of oxidative stress and is closely associated with cognitive decline in neurodegenerative diseases. The mechanism/s behind 12/15 LOX involvement in cognitive deficits remain obscure. The current study has been designed to investigate the underlying role of 12/15LOX and effect of 12/15 LOX inhibition on hypobaric hypoxia induced memory impairment and cholinergic deficits. Male Balb/c mice subjected to simulated hypobaric hypoxia/reoxygenation condition for 3 days showed marked working memory impairment concomitant with hippocampal neuronal damage and malondialdehyde production which were significantly attenuated by baicalein, a specific inhibitor of 12/15LOX...
July 15, 2017: Neuroscience
https://www.readbyqxmd.com/read/28714516/-epigallocatechin-3-gallate-attenuates-myocardial-injury-induced-by-ischemia-reperfusion-in-diabetic-rats-and-in-h9c2-cells-under-hyperglycemic-conditions
#4
Yang Wu, Zhong-Yuan Xia, Bo Zhao, Yan Leng, Juan Dou, Qing-Tao Meng, Shao-Qing Lei, Zhi-Ze Chen, Jie Zhu
(-)-Epigallocatechin gallate (EGCG) exerts multiple beneficial effects on cardiovascular performance. In this study, we aimed to examine the effects of EGCG on diabetic cardiomyopathy during myocardial ischemia/reperfusion (I/R) injury. EGCG (100 mg/kg/day) was administered at week 6 for 2 weeks to diabetic rats following the induction of type 1 diabetes by streptozotocin (STZ). At the end of week 8, the animals were subjected to myocardial I/R injury. The EGCG-elicited structural and functional effects were analyzed...
August 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28713956/mdg%C3%A2-1-inhibits-h2o2%C3%A2-induced-apoptosis-and-inflammation-in-human-umbilical-vein-endothelial-cells
#5
Luo-Cheng Li, Zhi-Wei Wang, Xiao-Ping Hu, Zhi-Yong Wu, Zhi-Peng Hu, Yong-Le Ruan
MDG‑1, a water‑soluble polysaccharide extracted from Ophiopogon japonicus, has been reported to serve a role in antimyocardial ischemia by protecting cardiomyocytes from hypoxia/reoxygenation‑induced damage. However, it remains unknown whether MDG‑1 protects human umbilical vein endothelial cells (HUVECs) against oxidative stress‑induced damage. In the present study, HUVECs were treated with hydrogen peroxide (H2O2) to establish an oxidative stress‑induced cell injury model. Treatment of HUVECs with different concentrations of H2O2 significantly attenuated cell viability and increased cell apoptosis in a time and dose‑dependent manner...
July 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28712401/-fisetin-alleviates-hypoxia-reoxygenation-injury-in-rat-hepatocytes-via-modulation-of-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#6
Junliang Pu, Lei Wan, Daofeng Zheng, Xufu Wei, Zhongjun Wu, Chengyong Tang
Objective To investigate the protective effect of fisetin (FIS) against hypoxia/reoxygenation (H/R) injury in rat hepatocytes and its mechanism. Methods H/R injury model of BRL-3A cells was established and the cells were pretreated with FIS. Survival rate was detected by CCK-8 assay. Cell apoptosis was measured by flow cytometry. The levels of ALT and AST were determined by microplate assay. The production of TNF-α and IL-1β were detected by ELISA. The mRNA and protein levels of TLR4 and NF-κBp65 were analyzed by quantitative real-time PCR and Western blotting, respectively...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28709867/attenuation-of-the-hypoxia-induced-mir-34a-protects-cardiomyocytes-through-maintenance-of-glucose-metabolism
#7
Zai-Cun Wang, Zun-Zhe Wang, Huan-Ju Ma, Chen-Chen Wang, Hua-Ting Wang
Ischemic injury in the heart is associated with death of cardiomyocytes and even after decades of research there is no appropriate therapeutic intervention to treat ischemic injury. The microRNA miR-34a is known to be induced in cardiomyocytes following ischemic injury. Another hallmark of ischemic injury is impaired glycolysis. The objective of the current study was to investigate the effects of short- and long-term exposure to hypoxia on miR-34a expression on apoptosis and regulation of key glycolysis metabolic enzymes...
July 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28708281/cooperation-of-hif-and-ncam-mediated-mechanisms-in-cell-viability-of-hippocampal-cultures-after-oxygen-glucose-deprivation
#8
Lushnikova Iryna, Nikandrova Yelyzaveta, Skibo Galyna
Neurodegenerative diseases of different genesis are the result of cellular damages including those caused by oxygen and glucose deficit. Neuronal survival or death in brain pathologies depends on a variety of interrelated molecular mechanisms. A key role in modulation of neuron viability belongs to HIF (hypoxia-inducible factor) and NCAM (neural cell adhesion molecules) signaling pathways. In this work we used organotypic and dissociated hippocampal cultures to analyze cell viability and HIF-1α immunopositive (HIF-1α(+) ) signal after 30 minutes oxygen-glucose deprivation (OGD) followed by 24 h of reoxygenation in the presence of FGL (synthetic NCAM-derived mimetic peptide)...
July 14, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28704337/alleviation-of-ischemia-reperfusion-injury-in-liver-steatosis-by-augmenter-of-liver-regeneration-alr-is-attributed-to-antioxidation-and-preservation-of-mitochondria
#9
Junhua Weng, Wen Li, Xiaowei Jia, Wei An
BACKGROUND: Fatty liver is 1 of the major impediments to liver surgery and liver transplantation because steatotic hepatocytes are more susceptible to ischemia-reperfusion injury (IRI). In this study, the effects of augmenter of liver regeneration (ALR) on hepatic IRI in steatotic mice were investigated. METHODS: In vivo, liver steatosis of mice was induced by feeding a methionine-choline deficient diet for 2 weeks. Three days prior to hepatic partial warm IRI, mice were transfected with the ALR-containing adenovirus...
July 13, 2017: Transplantation
https://www.readbyqxmd.com/read/28699284/a-monocyte-tnf-endothelial-activation-axis-in-sickle-transgenic-mice-therapeutic-benefit-from-tnf-blockade
#10
Anna Solovey, Arif Somani, John D Belcher, Liming Milbauer, Lucile Vincent, Rafal Pawlinski, Karl A Nath, Robert J Kelm, Nigel Mackman, M Gerard O'Sullivan, Kalpna Gupta, Gregory M Vercellotti, Robert P Hebbel
Elaboration of tumor necrosis factor (TNF) is a very early event in development of ischemia/reperfusion injury pathophysiology. Therefore, TNF may be a prominent mediator of endothelial cell and vascular wall dysfunction in sickle cell anemia, a hypothesis we addressed using NY1DD, S+S(Antilles) , and SS-BERK sickle transgenic mice. Transfusion experiments revealed participation of abnormally activated blood monocytes exerting an endothelial activating effect, dependent upon Egr-1 in both vessel wall and blood cells, and upon NFκB(p50) in a blood cell only...
July 11, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28688659/indole-3-carbinol-is-a-potent-inhibitor-of-ischemia-reperfusion-induced-inflammation
#11
Emmanuel Ampofo, Nico Lachnitt, Jeannette Rudzitis-Auth, Beate M Schmitt, Michael D Menger, Matthias W Laschke
BACKGROUND: Ischemia-reperfusion (I/R) induces tissue inflammation, which is characterized by an increased leukocyte-endothelial cell interaction and leukocyte transmigration. These processes are mediated by the activation of the nuclear factor (NF)κB signaling pathway, resulting in an elevated expression of specific adhesion molecules. The phytochemical indole-3-carbinol (I3C) has been shown to exert anti-inflammatory effects by interfering with NFκB signal transduction. The aim of the present study was to investigate whether I3C is capable of counteracting the pathogenesis of I/R injury...
July 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28685422/chronic-diseases-as-barriers-to-oxygen-delivery-a-unifying-hypothesis-of-tissue-reoxygenation-therapy
#12
G A Perdrizet
Modern medical practice has resulted in the accumulation of a growing number of incurable chronic diseases, many of which are inflammatory in nature. Inflammation establishes a hypoxic microenvironment within tissues, a condition of inflammatory hypoxia (IH). Tissues thus affected become severely compromised, are unable to elicit adaptive responses and eventually develop fibrosis and fixed microvascular deficits. Previous work has demonstrated that tissue hypoxia exits even within the simple human model of self-resolving inflammation, the tuberculin reaction...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28678198/virus-host-cell-crosstalk-in-hypoxic-hpv-positive-cancer-cells
#13
REVIEW
Karin Hoppe-Seyler, Julia Mändl, Svenja Adrian, Bianca J Kuhn, Felix Hoppe-Seyler
Oncogenic types of human papillomaviruses (HPVs) are major human carcinogens. The expression of the viral E6/E7 oncogenes plays a key role for HPV-linked oncogenesis. It recently has been found that low oxygen concentrations ("hypoxia"), as present in sub-regions of HPV-positive cancers, strongly affect the interplay between the HPV oncogenes and their transformed host cell. As a result, a state of dormancy is induced in hypoxic HPV-positive cancer cells, which is characterized by a shutdown of viral oncogene expression and a proliferative arrest that can be reversed by reoxygenation...
July 5, 2017: Viruses
https://www.readbyqxmd.com/read/28677776/sasanquasaponin-induces-increase-of-cl%C3%A2-hco3%C3%A2-exchange-of-anion-exchanger-3-and-promotes-intracellular-cl%C3%A2-efflux-in-hypoxia-reoxygenation-cardiomyocytes
#14
Ling-Yu Qiu, Guang-Ling Duan, Yu-Feng Yan, Yuan-Yuan Li, Huan Wang, Ling Xiao, Zhang-Ping Liao, He-Ping Chen
Anion exchanger 3 (AE3) is known to serve crucial roles in maintaining intracellular chloride homeostasis by facilitating the reversible electroneutral exchange of Cl‑ for HCO3‑ across the plasma membrane. Our previous studies reported that sasanquasaponin (SQS) can inhibit hypoxia/reoxygenation (H/R)‑induced elevation of intracellular Cl‑ concentration ([Cl‑]i) and elicit cardioprotection by favoring Cl‑/HCO3‑ exchange of AE3. However, the molecular basis for SQS‑induced increase of Cl‑/HCO3‑ exchange of AE3 remains unclear...
June 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28672986/dexmedetomidine-attenuates-hypoxia-reoxygenation-injury-in-primary-neonatal-rat-cardiomyocytes
#15
Ke Peng, Yun Qiu, Jian Li, Zhao-Cai Zhang, Fu-Hai Ji
Systemic administration of dexmedetomidine provides cardioprotection against ischemia/reperfusion (I/R) injury; however, the direct effects of dexmedetomidine on cardiomyocytes have not been clarified. The present study investigated the effects of dexmedetomidine on primary neonatal rat cardiomyocytes under hypoxic/reoxygenation (H/R) conditions. In order to simulate in vivo I/R injury, primary neonatal rat cardiomyocytes were cultured under hypoxic conditions for 1 h and subsequently reoxygenated for 24 h...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28672952/emodin-protects-against-oxidative-stress-and-apoptosis-in-hk-2-renal-tubular-epithelial-cells-after-hypoxia-reoxygenation
#16
Hui Chen, Ri-Sheng Huang, Xian-Xian Yu, Qiong Ye, Lu-Lu Pan, Guo-Jian Shao, Jing Pan
The aim of the present study was to determine the effects of emodin, a natural compound with antioxidant properties, on oxidative stress and apoptosis induced by hypoxia/reoxygenation (H/R) in HK-2 human renal tubular cells. In HK-2 cells subjected to H/R, it was observed that pre-treatment with emodin lead to an increase in cellular viability and a reduction in the rate of apoptosis and the B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 ratio. H/R alone caused a significant increase in the levels of reactive oxygen species and malondialdehyde (P<0...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28672921/the-mechanism-of-blood-concentrations-of-the-shenqi-pill-repairing-injured-epithelial-cells-of-renal-tubular-in-vitro
#17
Fei Huang, Wenfeng Ma
In the present study, we investigated the best blood concentration of the mechanism of Shenqi pill repairing the injured epithelial cells of renal tubular in vitro. First, the injured hypoxia/reoxygenation model of rat proximal renal tubular epithelial cell strain (NRK-52E) was established. The animals were divided randomly into control, model, low concentration (5 µg/ml), moderate concentration (10 µg/ml) and high concentration (20 µg/ml) groups. The apoptotic rate was measured with flow cytometry and Jag2/Notch2/hes1 mRNA, and the protein expression was measured for 1, 3 and 7 days...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28671970/cardioprotective-effects-of-pkg-activation-by-soluble-gc-activator-bay-60-2770-in-ischemia-reperfusion-injured-rat-hearts
#18
Kyung Hye Lee, So-Ra Lee, Haneul Cho, Jong Shin Woo, Jung Hee Kang, Yun-Mi Jeong, Xian Wu Cheng, Woo-Shik Kim, Weon Kim
Soluble guanylate cyclase (sGC) has been suggested as a therapeutic target for cardiac ischemia-reperfusion (IR) injury. Until now, the molecular mechanism of BAY 60-2770, a sGC activator, in cardiac IR injury has not been assessed. To identify the cardioprotective effects of BAY 60-2770 in IR-injured rat hearts, IR injury was established by occlusion of LAD for 40 min and reperfusion for 7 days, and the effects of BAY 60-2770 on myocardial protection were assessed by echocardiography and TTC staining. 5 nM and 5 μM of BAY 60-2770 were perfused into isolated rat hearts in a Langendorff system...
2017: PloS One
https://www.readbyqxmd.com/read/28666275/curcumin-protects-against-hepatic-ischemia-reperfusion-induced-injury-through-inhibiting-tlr4-nf-%C3%AE%C2%BAb-pathway
#19
Lu Wang, Ning Li, Dongdong Lin, Yunjin Zang
The TLR4/NF-κB pathway had important roles in hepatic ischemia/reperfusion (I/R) injury. In this study, we reported a protective effect of curcumin against hepatic I/R injury via TLR4/NF-κB pathway. Curcumin significantly inhibited cell apoptosis, and decreased levels of LDH and production of TNF-a, IL-1b, and IL-6 in the cell supernatant. In addition, curcumin ameliorated elevated TLR4 and NF-κB caused by hypoxia/reoxygenation stimulation in BRL-3A cells. In vivo assays revealed that curcumin reduce levels of ALT and AST, and reversed TLR4/NF-κB signaling pathway caused by hepatic I/R stimulation in liver tissues...
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28661424/vitamin-c-and-microvascular-dysfunction-in-systemic-inflammation
#20
REVIEW
Karel Tyml
Sepsis, life-threatening organ dysfunction caused by a dysfunctional host response to infection, is associated with high mortality. A promising strategy to improve the outcome is to inject patients intravenously with ascorbate (vitamin C). In animal models of sepsis, this injection improves survival and, among others, the microvascular function. This review examines our recent work addressing ascorbate's ability to inhibit arteriolar dysfunction and capillary plugging in sepsis. Arteriolar dysfunction includes impaired vasoconstriction/dilation (previously reviewed) and impaired conduction of vasoconstriction/dilation along the arteriole...
June 29, 2017: Antioxidants (Basel, Switzerland)
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