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Liver progenitor

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https://www.readbyqxmd.com/read/29220096/hepatic-progenitor-cells-in-metastatic-liver-carcinomas
#1
Ioanna Delladetsima, Stratigoula Sakellariou, Olivier Govaere, Elpida Poulaki, Evangelos Felekouras, Dina Tiniakos
AIMS: Hepatic progenitor cells (HPCs) are activated in various liver diseases but their role in carcinomatous environment remains unknown. We aimed to identify the possible presence and topography of HPCs in liver metastases. METHODS: We examined 14 liver resection specimens for colorectal adenocarcinoma (CRC) (n=13) and anal squamous cell carcinoma (SCC) (n=1) metastases. Immunohistochemical markers of colonic (keratin 20-K20, CDX2) and squamous cell origin (p63), HPC (K19, CD56) and stem cell (CD44) markers, and the biliary marker K7 which may also highlight HPCs were applied on routinely processed tissue sections...
December 8, 2017: Histopathology
https://www.readbyqxmd.com/read/29212014/massive-and-reproducible-production-of-liver-buds-entirely-from-human-pluripotent-stem-cells
#2
Takanori Takebe, Keisuke Sekine, Masaki Kimura, Emi Yoshizawa, Satoru Ayano, Masaru Koido, Shizuka Funayama, Noriko Nakanishi, Tomoko Hisai, Tatsuya Kobayashi, Toshiharu Kasai, Rina Kitada, Akira Mori, Hiroaki Ayabe, Yoko Ejiri, Naoki Amimoto, Yosuke Yamazaki, Shimpei Ogawa, Momotaro Ishikawa, Yasujiro Kiyota, Yasuhiko Sato, Kohei Nozawa, Satoshi Okamoto, Yasuharu Ueno, Hideki Taniguchi
Organoid technology provides a revolutionary paradigm toward therapy but has yet to be applied in humans, mainly because of reproducibility and scalability challenges. Here, we overcome these limitations by evolving a scalable organ bud production platform entirely from human induced pluripotent stem cells (iPSC). By conducting massive "reverse" screen experiments, we identified three progenitor populations that can effectively generate liver buds in a highly reproducible manner: hepatic endoderm, endothelium, and septum mesenchyme...
December 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/29206089/papillomatosis-of-the-biliary-tree-and-gallbladder-successful-treatment-with-repeated-resection-and-liver-transplant
#3
Vor Luvira, Ake Pugkhem, Vajarabhongsa Bhudhisawasdi, Thong-Ueb Uttaravichien, Anan Sripanuskul, Jongruk Pongskul, Chawalit Pairojkul
Intraductal papillary neoplasm of the bile duct is now considered to be a specific type of bile duct tumor. The progenitor cells of intraductal papillary neoplasms of the bile duct are located in the peribiliary gland, which are distributed along the intrahepatic bile duct, extrahepatic bile duct, and gallbladder; therefore, these neoplasms could arise in any area. The mainstay of treatment for patients with intraductal papillary neoplasm of the bile duct is complete surgical resection. Neoplasms involving both lobes of the liver can only be treated with liver transplant...
December 5, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29204185/bioengineering-considerations-in-liver-regenerative-medicine
#4
REVIEW
Ogechi Ogoke, Janet Oluwole, Natesh Parashurama
Background: Liver disease contributes significantly to global disease burden and is associated with rising incidence and escalating costs. It is likely that innovative approaches, arising from the emerging field of liver regenerative medicine, will counter these trends. Main body: Liver regenerative medicine is a rapidly expanding field based on a rich history of basic investigations into the nature of liver structure, physiology, development, regeneration, and function...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29202695/single-cell-rna-seq-analysis-reveals-dynamic-trajectories-during-mouse-liver-development
#5
Xianbin Su, Yi Shi, Xin Zou, Zhao-Ning Lu, Gangcai Xie, Jean Y H Yang, Chong-Chao Wu, Xiao-Fang Cui, Kun-Yan He, Qing Luo, Yu-Lan Qu, Na Wang, Lan Wang, Ze-Guang Han
BACKGROUND: The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking. RESULTS: We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 507 cells randomly selected from seven stages between embryonic day 11.5 and postnatal day 2.5 during mouse liver development, and also 52 Epcam-positive cholangiocytes from postnatal day 3...
December 4, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29198531/laparoscopic-sleeve-gastrectomy-improves-nonalcoholic-fatty-liver-disease-related-liver-damage-in-adolescents-by-reshaping-cellular-interactions-and-hepatic-adipocytokine-production
#6
Valerio Nobili, Guido Carpino, Francesco De Peppo, Romina Caccamo, Antonella Mosca, Ilaria Romito, Diletta Overi, Antonio Franchitto, Paolo Onori, Anna Alisi, Eugenio Gaudio
OBJECTIVES: To investigate whether the modulation of local cellular cross-talks and the modification of hepatic adipocytokine expression could mechanistically explain the improvement of liver histopathology after laparoscopic sleeve gastrectomy (LSG) in adolescents with nonalcoholic fatty liver disease (NAFLD). STUDY DESIGN: Twenty obese (body mass index of ≥35 kg/m2) adolescents who underwent LSG and with biopsy-proven NAFLD were included. At baseline (T0) and 1 year after treatment, patients underwent clinical evaluation, blood tests, and liver biopsy...
November 30, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/29184906/parvovirus-b19-in-the-context-of-hematopoietic-stem-cell-transplantation-evaluating-cell-donors-and-recipients
#7
Bianca E Gama, Vanessa E Emmel, Michelle Oliveira-Silva, Luciana M Gutiyama, Leonardo Arcuri, Marta Colares, Rita de Cássia Tavares, Luis F Bouzas, Eliana Abdelhay, Rocio Hassan
Background: Parvovirus B19 (B19V) is a common human pathogen, member of the family Parvoviridae. Typically, B19V has been found to infect erythroid progenitors and cause hematological disorders, such as anemia and aplastic crisis. However, the persistence of genomic deoxyribonucleic acid (DNA) has been demonstrated in tonsils, liver, skin, brain, synovial, and testicular tissues as well as bone marrow, for both symptomatic and asymptomatic subjects. Although the molecular and cellular mechanisms of persistence remain undefined, it raises questions about potential virus transmissibility and its effects in the context of allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients...
November 2017: Transplantation Direct
https://www.readbyqxmd.com/read/29181772/liver-cell-therapy-is-this-the-end-of-the-beginning
#8
REVIEW
Salamah M Alwahsh, Hassan Rashidi, David C Hay
The prevalence of liver diseases is increasing globally. Orthotopic liver transplantation is widely used to treat liver disease upon organ failure. The complexity of this procedure and finite numbers of healthy organ donors have prompted research into alternative therapeutic options to treat liver disease. This includes the transplantation of liver cells to promote regeneration. While successful, the routine supply of good quality human liver cells is limited. Therefore, renewable and scalable sources of these cells are sought...
November 27, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29179659/dyrk1a-is-a-regulator-of-s-phase-entry-in-hepatic-progenitor-cells
#9
Hedwig Suzanne Kruitwagen, Bart Westendorp, Cornelia S Viebahn, Krista Post, Monique E van Wolferen, Loes A Oosterhoff, David A Egan, Jean-Maurice Delabar, Mathilda Jm Toussaint, Baukje A Schotanus, Alain de Bruin, Jan Rothuizen, Louis C Penning, Bart Spee
Hepatic progenitor cells (HPCs) are adult liver stem cells that act as second line of defense in liver regeneration. They are normally quiescent, but in case of severe liver damage HPC proliferation is triggered by external activation mechanisms from their niche. Although several important pro-proliferative mechanisms have been described, it is not known which key intracellular regulators govern the switch between HPC quiescence and active cell cycle. We performed a high throughput kinome siRNA screen in HepaRG cells, a HPC-like cell line, and evaluated the effect on proliferation with a 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay...
November 28, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29176797/cytokines-hepatic-cell-profiling-and-cell-interactions-during-bone-marrow-cell-therapy-for-liver-fibrosis-in-cholestatic-mice
#10
Daphne Pinheiro, Luana Leirós, Juliana Barbosa Torreão Dáu, Ana Carolina Stumbo, Alessandra Alves Thole, Erika Afonso Costa Cortez, Carlos Alberto Mandarim-de-Lacerda, Lais de Carvalho, Simone Nunes de Carvalho
Bone marrow cells (BMC) migrate to the injured liver after transplantation, contributing to regeneration through multiple pathways, but mechanisms involved are unclear. This work aimed to study BMC migration, characterize cytokine profile, cell populations and proliferation in mice with liver fibrosis transplanted with GFP+ BMC. Confocal microscopy analysis showed GFP+ BMC near regions expressing HGF and SDF-1 in the fibrotic liver. Impaired liver cell proliferation in fibrotic groups was restored after BMC transplantation...
2017: PloS One
https://www.readbyqxmd.com/read/29170436/fate-tracing-of-hepatocytes-in-mouse-liver
#11
Xiaowen Gu, Danyi Huang, Lei Ci, Jiahao Shi, Mengjie Zhang, Hua Yang, Zhugang Wang, Zhejin Sheng, Ruilin Sun, Jian Fei
Hepatocytes perform most of the functions of the liver and are considered terminally differentiated cells. Recently, it has been suggested that hepatocytes might have the potential to transdifferentiate or dedifferentiate under physiological or pathological conditions in vivo. Epithelial-mesenchymal transition of hepatocytes in liver fibrosis has also been proposed. However, these findings have not been fully confirmed. In this study, hepatocytes were genetically labelled for cell fate tracing using lacZ via the tamoxifen-induced CreERT/loxP system...
November 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29155718/the-murine-choline-deficient-ethionine-supplemented-cde-diet-model-of-chronic-liver-injury
#12
Jully Gogoi-Tiwari, Julia Köhn-Gaone, Corey Giles, Dirk Schmidt-Arras, Francis D Gratte, Caryn L Elsegood, Geoffrey W McCaughan, Grant A Ramm, John K Olynyk, Janina E E Tirnitz-Parker
Chronic liver diseases, such as viral hepatitis, alcoholic liver disease, or non-alcoholic fatty liver disease, are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, liver progenitor cell proliferation, and fibrosis. The end-stage of every chronic liver disease is cirrhosis, a major risk factor for the development of hepatocellular carcinoma. To study processes regulating disease initiation, establishment, and progression, several animal models are used in laboratories...
October 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155473/relationship-between-hepatic-progenitor-cells-and-stellate-cells-in-chronic-hepatitis-c-genotype-4
#13
Thanaa El Sayed Ahmed Helal, Nermine Ahmed Ehsan, Nehal Ahmed Radwan, Eman Abdelsameea
Hepatitis C virus (HCV) infection represents a major health problem in many areas of the world, especially Egypt. Hepatic progenitor cells (HPCs) and hepatic stellate cells (HSCs) have been implicated in fibrosis progression in chronic HCV. The aim of this study was to investigate the role of HPCs and HSCs in chronic HCV infection and the relationship between both cell types. This retrospective study was conducted on 100 chronic HCV patients. Immunohistochemistry was performed on liver tissue sections for cytokeratin 19 (progenitor cell markers), smooth muscle actin (stellate cell markers), matrix metalloproteinase-9 (MMP-9), and transforming growth factor beta (TGF-ß)...
November 20, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29152108/microrna-31-suppresses-the-self-renewal-capability-of-%C3%AE-2%C3%AE-1-liver-tumor-initiating-cells-by-targeting-isl1
#14
Yuan Zhang, Wei Zhao, Haibo Han, Sheng Li, Dongji Chen, Zhiqian Zhang
Accumulating evidence demonstrates that miRNAs, a class of small non-coding RNAs, are involved in the regulation of tumor-initiating cells (TICs) which are considered to be the origin of cancer development according to the cancer stem cell hypothesis. We have previously identified that miR-31 may play suppressive roles in α2δ1(+) hepatocellular carcinoma (HCC) TICs. Here, we confirm that the expression of miR-31 is significantly downregulated in α2δ1(+) HCC TICs. Overexpression of miR-31 in α2δ1(+) HCC TICs results in significant suppression of the self-renewal and tumorigenicity abilities of these cells...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29150936/the-roles-of-wnt-%C3%AE-catenin-pathway-in-tissue-development-and-regenerative-medicine
#15
REVIEW
Maryam Majidinia, Javad Aghazadeh, Rana Jahanban-Esfahlani, Bahman Yousefi
Regenerative medicine is a translational field which combines tissue engineering and molecular biology to construct spare organs or help injured or defective tissues to regenerate or restore their normal functions. This is particularly important with specific organs such as heart, central nervous system, retina or limbs which possess very limited regenerative capacity. As such, regenerative medicine has received peculiar attention in the last decade. In this regard, Wnt/β-catenin signaling pathway has been subject to intensive research, since it plays many essential roles in the regulation of the progenitor cell fate, developmental decisions, proliferation during embryonic development, and adult tissue homeostasis...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29150621/pnpla3-variant-and-portal-periportal-histological-pattern-in-patients-with-biopsy-proven-non-alcoholic-fatty-liver-disease-a-possible-role-for-oxidative-stress
#16
Guido Carpino, Daniele Pastori, Francesco Baratta, Diletta Overi, Giancarlo Labbadia, Licia Polimeni, Alessia Di Costanzo, Gaetano Pannitteri, Roberto Carnevale, Maria Del Ben, Marcello Arca, Francesco Violi, Francesco Angelico, Eugenio Gaudio
Pathogenesis of non-alcoholic fatty liver disease (NAFLD) is influenced by predisposing genetic variations, dysmetabolism, systemic oxidative stress, and local cellular and molecular cross-talks. Patatin-like phospholipase domain containing 3 (PNPLA3) gene I148M variant is a known determinant of NAFLD. Aims were to evaluate whether PNPLA3 I148M variant was associated with a specific histological pattern, hepatic stem/progenitor cell (HpSC) niche activation and serum oxidative stress markers. Liver biopsies were obtained from 54 NAFLD patients...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29141455/methionine-adenosyltransferases-in-cancers-mechanisms-of-dysregulation-and-implications-for-therapy
#17
Lauren Y Maldonado, Diana Arsene, José M Mato, Shelly C Lu
Methionine adenosyltransferase genes encode enzymes responsible for the biosynthesis of S-adenosylmethionine, the principal biological methyl donor and precursor of polyamines and glutathione. Mammalian cells express three genes - MAT1A, MAT2A, and MAT2B - with distinct expression and functions. MAT1A is mainly expressed in the liver and maintains the differentiated states of both hepatocytes and bile duct epithelial cells. Conversely, MAT2A and MAT2B are widely distributed in non-parenchymal cells of the liver and extrahepatic tissues...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29140985/notch-signaling-and-progenitor-ductular-reaction-in-steatohepatitis
#18
Carola M Morell, Romina Fiorotto, Marica Meroni, Aileen Raizner, Barbara Torsello, Massimiliano Cadamuro, Gaia Spagnuolo, Eleanna Kaffe, Salvatore Sutti, Emanuele Albano, Mario Strazzabosco
BACKGROUND AND OBJECTIVE: Persistent hepatic progenitor cells (HPC) activation resulting in ductular reaction (DR) is responsible for pathologic liver repair in cholangiopathies. Also, HPC/DR expansion correlates with fibrosis in several chronic liver diseases, including steatohepatitis. Increasing evidence indicates Notch signaling as a key regulator of HPC/DR response in biliary and more in general liver injuries. Therefore, we aimed to investigate the role of Notch during HPC/DR activation in a mouse model of steatohepatitis...
2017: PloS One
https://www.readbyqxmd.com/read/29138811/high-expression-of-mage-a9-contributes-to-stemness-and-malignancy-of-human-hepatocellular-carcinoma
#19
Youping Wei, Yanqin Wang, Jing Gong, Lihua Rao, Zhiwei Wu, Teng Nie, Dongling Shi, Liming Zhang
MAGE-A9, a well-characterized cancer testis antigen (CTA), belongs to a member of melanoma antigen gene (MAGE) family. In human malignancies, aberrant expression of MAGE genes correlated with poor clinical prognosis, increased tumor growth, metastases, and enrichment in stem cell populations of certain cancers. Cancer stem cells (CSCs) have been proposed to contribute to the major malignant phenotypes of liver cancer, including recurrence, metastasis and chemoresistance. However, expression and potential role of MAGE-A9 in liver cancer stem cells (LCSCs) still remain unclear...
November 9, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29131159/enhancing-the-precision-of-genetic-lineage-tracing-using-dual-recombinases
#20
Lingjuan He, Yan Li, Yi Li, Wenjuan Pu, Xiuzhen Huang, Xueying Tian, Yue Wang, Hui Zhang, Qiaozhen Liu, Libo Zhang, Huan Zhao, Juan Tang, Hongbin Ji, Dongqing Cai, Zhibo Han, Zhongchao Han, Yu Nie, Shengshou Hu, Qing-Dong Wang, Ruilin Sun, Jian Fei, Fengchao Wang, Ting Chen, Yan Yan, Hefeng Huang, William T Pu, Bin Zhou
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing...
November 13, 2017: Nature Medicine
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