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M R Kuznetsov, I V Reshetov, L A Magnitskiĭ, Vasiliev V, I P Marchenko, A D Matveev, A A Lugovoĭ, M S Tverskaia
Knowledge of anatomical variations of the division of the popliteal artery and specific lesions of the arterial segment involved is necessary for the surgeon, traumatologist-orthopaedist, vascular surgeon. The article contains a review of recent literature data on the prevalence of different variants of branching patterns of the arteries of the crus, also providing a detailed discussion of embryological development of the arterial system of the lower extremities, in many ways explaining the appearance of rare pathologies of the popliteal artery, such as for example popliteal artery entrapment syndrome...
2018: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
Hiroki Shibutani, Yuzo Akita, Yohei Oishi, Hiroyuki Sueyoshi, Yu Mukai, Kotaro Yutaka, Yumie Matsui, Masahiro Yoshinaga, Masahiro Karakawa
BACKGROUND: A new complication, longitudinal stent deformation (LSD), is increasingly reported with recent intracoronary stent designs. We experienced unusual cases of distal LSD caused by entrapment of a Lacrosse® non-slip element (NSE) balloon (Goodman Co., Ltd., Nagoya, Japan), which comes with three flexible nylon elements to prevent slippage. Accordingly, the aim of this study is to report the clinical experience of distal LSD caused by the NSE in our center and to investigate the incidence and mechanisms involved...
June 20, 2018: Cardiology Journal
Emad Kordbacheh, Shahram Nazarian, Davoud Sadeghi, Abbas Hajizadeh
Objectives: Enterotoxigenic Escherichia coli (ETEC) is known as the most common bacterial causes of diarrheal diseases related to morbidity and mortality. Heat-labile enterotoxin (LT) is a part of major virulence factors in ETEC pathogenesis. Antigen entrapment into nanoparticles (NPs) can protect them and enhance their immunogenicity. Materials and Methods: In the present study, recombinant LTB protein was expressed in E. coli BL21 (DE3) and purified by an Ni-NTA agarose column...
May 2018: Iranian Journal of Basic Medical Sciences
Julia Pfanzelter, Serge Mostowy, Michael Way
Septins are conserved components of the cytoskeleton that play important roles in many fundamental cellular processes including division, migration, and membrane trafficking. Septins can also inhibit bacterial infection by forming cage-like structures around pathogens such as Shigella We found that septins are recruited to vaccinia virus immediately after its fusion with the plasma membrane during viral egress. RNA interference-mediated depletion of septins increases virus release and cell-to-cell spread, as well as actin tail formation...
June 19, 2018: Journal of Cell Biology
Diana Edwar Aziz, Aly Ahmed Abdelbary, Abdelhalim Ibrahim Elassasy
BACKGROUND: Niosomes are surfactant-based vesicular nanosystems that proved their efficiency in transdermal delivery by overcoming skin inherent anatomic barrier; startum corneum. Central composite design is an efficient tool for developing and optimizing niosomal formulations using fewer experiments. OBJECTIVE: The objective of this study was to prepare niosomes as a transdermal delivery system of diacerein using film hydration technique, employing central composite design, for avoiding its oral gastrointestinal problems...
June 18, 2018: Current Drug Delivery
Cristina Carucci, Larah Bruen, Victoria Gascon Perez, Francesca Paradisi, Edmond Magner
The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and ace-tonitrile...
June 19, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Mei Zhang, Yajun Tang, Zhenhua Zhu, He Zhao, Jihang Yao, Dahui Sun
In this study, we fabricated paclitaxel (PTX) and etoposide (ETP) loaded Poly (lactic-co-glycolic acid) (PLGA) microspheres with core-shell structures and particle sizes ranging from 1 to 4 µm by coaxial electrospraying. The microspheres were analyzed by scanning electron microscopy (SEM), transmission electron microscopy (TEM). The drug loading rate and entrapment efficiency of the microspheres were detected by high performance liquid chromatograph (HPLC). Moreover, the drug release profiles and degradation of drug-loaded PLGA microspheres in vitro were investigated, respectively...
June 19, 2018: Journal of Biomaterials Science. Polymer Edition
Carles Fuertes-Espinosa, Alejandra Gómez-Torres, Roser Morales-Martínez, Antonio Rodríguez-Fortea, Cristina García-Simón, Felipe Gándara, Inhar Imaz, Judith Juanhuix, Daniel Maspoch, Josep M Poblet, Luis Echegoyen, Xavi Ribas
Supramolecular nanocapsule 1·(BArF)8 is able to sequentially and selectively entrap recently discovered U2@C80 and unprecedented Sc2CU@C80, simply by soaking crystals of 1·(BArF)8 in a toluene solution of arc-produced soot. These species, selectively and stepwise absorbed by 1·(BArF)8, are easily released, obtaining highly pure fractions of U2@C80 and Sc2CU@C80 in one step. Sc2CU@C80 represents the first example of a mixed metal actinide-based endohedral metallofullerene (EMF). Remarkably, the host-guest studies revealed that 1·(BArF)8 is able to discriminate EMFs with the same carbon cage but with different encapsulated cluster and computational studies provide support for these observations...
June 19, 2018: Angewandte Chemie
Fengying Shao, Lianhua Zhang, Lei Jiao, Xiaoying Wang, Luyang Miao, He Li, Feimeng Zhou
An enzyme-free titer plate-based colorimetric assay utilizing functionalized mesoporous silica nanoparticles (MSNs) entrapping pH-indicator molecules has been developed. Pores in the silica nanoparticles were functionalized with phenyltrimethyloxysilane so that pH indicator molecules (thymolphthalein or TP in the present case) can be tightly entrapped through ππ conjugation. To detect prostate specific antigen (PSA), the TP-containing MSNs were coated with polyethylenimine (PEI), which favors the attachment of the positively charged secondary anti-PSA antibody...
June 19, 2018: Analytical Chemistry
Wógenes N Oliveira, Lucas Amaral-Machado, Everton N Alencar, Henrique R Marcelino, Julieta Genre, Walicyranison P Silva-Rocha, Amanda D Gondim, Guilherme M Chaves, Matheus F Fernandes-Pedrosa, Eryvaldo Socrates T Egito
Amphotericin B (AmB), a potent antifungal drug, presents physicochemical characteristics that impair the development of suitable dosage forms. In order to overcome the AmB insolubility, several lipid carriers such as microemulsions have been developed. In this context, the bullfrog oil stands out as an eligible oily phase component, since its cholesterol composition may favor the AmB incorporation. Thus, the aim of this study was to develop a microemulsion based on bullfrog oil containing AmB. Moreover, its thermal stability, antifungal activity, and cytotoxicity in vitro were evaluated...
June 18, 2018: AAPS PharmSciTech
Michelle B Chen, Cynthia Hajal, David C Benjamin, Cathy Yu, Hesham Azizgolshani, Richard O Hynes, Roger D Kamm
Systemic inflammation occurring around the course of tumor progression and treatment are often correlated with adverse oncological outcomes. As such, it is suspected that neutrophils, the first line of defense against infection, may play important roles in linking inflammation and metastatic seeding. To decipher the dynamic roles of inflamed neutrophils during hematogenous dissemination, we employ a multiplexed microfluidic model of the human microvasculature enabling physiologically relevant transport of circulating cells combined with real-time, high spatial resolution observation of heterotypic cell-cell interactions...
June 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
Pang-Hu Zhou, Bo Qiu, Rong-Hui Deng, Hua-Jie Li, Xiong-Feng Xu, Xi-Fu Shang
BACKGROUND/AIMS: Interleukin (IL)-1β plays an essential role in the pathophysiology of osteoarthritis (OA). Cytokine response modifier A (CrmA) can prevent the generation of active IL-1β. This study aimed to explore the chondroprotective effects of hyaluronic acid-chitosan nanoparticles containing plasmid DNA encoding CrmA (HA/CS-CrmA) in a rat OA model. METHODS: HA/CS-CrmA nanoparticles were synthesized through the complex coacervation of cationic polymers. The characteristics, toxicity, and transfection of the nanoparticles were investigated...
June 15, 2018: Cellular Physiology and Biochemistry
Quanjing Mei, Peipei Luo, Yi Zuo, Jidong Li, Qin Zou, Yubao Li, Dianming Jiang, Yaning Wang
Purpose: Mycobacterium tuberculosis is a serious public health problem affecting hundreds of millions of elderly people worldwide, which is difficult to be treated by traditional methods because of the peculiarity of skeletal system and liver damage caused by high-dose administration. In this research, a porous drug release system has been attempted to encapsulate rifampicin (RIF) into poly (ε-caprolactone) (PCL) microspheres to improve the efficacy and benefit of anti-tuberculosis drug in skeletal system...
2018: Drug Design, Development and Therapy
Barbara Wienen-Schmidt, Hendrik R A Jonker, Tobias Wulsdorf, Hans-Dieter Gerber, Krishna Saxena, Denis Kudlinzki, Sridhar Sreeramulu, Giacomo Parigi, Claudio Luchinat, Andreas Heine, Harald Schwalbe, Gerhard Klebe
Biophysical parameters can accelerate drug development, e.g. rigid ligands may reduce entropic penal-ty and improve binding affinity. We studied systematically the impact of ligand rigidification on ther-modynamics using a series of fasudil derivatives inhibiting protein kinase A by crystallography, iso-thermal titration calorimetry, nuclear magnetic resonance and molecular dynamics simulations. The ligands varied in their internal degrees of freedom but conserve the number of heteroatoms. Counter-intuitively, the most flexible ligand displays the entropically most favored binding...
June 16, 2018: Journal of Medicinal Chemistry
Arash Babaei-Ghazani, Peyman Roomizadeh, Golshan Sanaei, Saeideh Najarzadeh-Mehdikhani, Kimia Habibi, Shahin Nikmanzar, Yadollah Kheyrollah
PURPOSE: The deep branch of the radial nerve (DBRN) enters the forearm as it passes under the arcade of Frohse. This is the most common entrapment site of the DBRN in the forearm. In this study, we investigated the ultrasonographic reference values for the diameters and cross-sectional area (CSA) of the DBRN at the level of the arcade of Frohse in a healthy sample of the population. METHODS: Sixty-five healthy Caucasian volunteers (130 nerves) were recruited for this study...
June 16, 2018: Journal of Ultrasound
Tingting Lv, Liang Xu, Guolin Wu, Cailong Li, Yibo Wen, Tao Zhang, Haijun Chen, Yu Gao
Chemotherapy is currently one of the promising therapeutic methods for non-small-cell lung cancer (NSCLC), but the emergence of multidrug resistance (MDR) is the greatest obstacle to efficient drug delivery for successful chemotherapy. Nanotechnology-based drug delivery holds great promise to promote intracellular drug delivery to reverse MDR. In this work, we used our previously synthesized ursolic acid (UA) derivative, FZU-03,010 (F3), to prepare nanodrugs of F3 with different architectures and study the role of the structure on the physiochemical properties and the biological effects against A549 and its PTX-resistant A549/PTX lung cancer cells...
June 13, 2018: European Journal of Pharmaceutical Sciences
Richa Shrivastava, Shruti Trivedi, Pankaj Kumar Singh, Mohammad Asif, Manish Kumar Chourasia, Amit Khanna, Smrati Bhadauria
Breast cancer is most frequently diagnosed cancer and fifth leading cause of death in women. About 20-30% of all breast cancers overexpress HER2/neu receptors. Lapatinib is a dual tyrosin kinase inhibitor of EGFR and HER2. It exhibits its anticancer effect via blocking intracellular domain of HER2 receptor in breast cancer. Lapatinib belongs to class II of BSC classification due to its poor solubility restricting its clinical application. Due to presence of HER2 receptor on cardiomyocytes, it is associated with generation of cardiotoxicity...
June 13, 2018: Biochemical and Biophysical Research Communications
Si-Qi Huang, Bei-Bei Han, Yan-Mei Li, Yong-Xiang Chen
Intracellular protein delivery is of significance for cellular protein analysis and therapeutic development, but remains challenging technically. Herein, we report a general and highly potent strategy for intracellular protein delivery based on commercially available cationic lipids. In this strategy, a designed double branching poly-glutamate tag is site-specifically attached onto the C-terminal of protein cargos via expressed protein ligation (EPL), which mediates the entrapment of proteins into cationic liposomes driven by electrostatic interaction...
June 13, 2018: Biochemical and Biophysical Research Communications
Johan L Å Nilsson, Anders Blomgren, Ulf J Nilsson, Edward D Högestätt, Lars Grundemar
Paracetamol overdosing may cause liver injury including fulminant liver failure due to generation of the toxic metabolites, N-acetyl-p-benzoquinone imine (NAPQI) and p-benzoquinone (p-BQ). Herein, the chelating agent, N,N'-Bis(2-mercaptoethyl)isophthalamide (NBMI), was examined for its potential ability to entrap NAPQI and p-BQ and to prevent paracetamol-induced liver injury. Both NBMI and the conventional paracetamol antidote N-Acetyl cysteine (NAC) were investigated with regard to their abilities to scavenge the NAPQI and p-BQ in a Transient Receptor Potential Ankyrin 1 (TRPA1)-dependent screening assay...
June 16, 2018: Basic & Clinical Pharmacology & Toxicology
Jessica Fernandes, M Vivek Ghate, Sanchari Basu Mallik, Shaila A Lewis
Saxagliptin (SAX) is dipeptidyl peptidase-4 enzyme inhibitor molecule now explored for its activity in the therapy of Alzheimer's disease. Being extremely hydrophilic, it is unable to permeate the blood-brain barrier by the conventional therapy modalities. Further repurposing the drug, SAX is associated with a reduction in the blood sugar level in the periphery. In the present study, the chitosan-L-valine conjugate was synthesized by carbodiimide chemistry. The conjugate was then used to prepare nanoparticles encapsulating SAX...
June 12, 2018: International Journal of Pharmaceutics
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