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Ye Fu, Wubin Wang, Xuejing Li, Yanli Liu, Yingying Niu, Bin Zhang, Jisheng Nie, Baolong Pan, Ruisheng Wang, Jin Yang
Benzo [a]pyrene (BaP) have been demonstrated to induce lung cancer risk in humans and many different animal models, with aberrant gene methylation as one of the epigenetic errors; however, the molecular mechanisms remain unclear. Here, we used three types of human lung-derived cells with BaP exposure as a model and attempted to investigate the long non-coding RNA (lncRNA) H19/S-adenosylhomocysteine hydrolase (SAHH) pathway that regulates gene methylation in vitro exposure to BaP. Results showed that compared to the controls, BaP-treated cells H19 expressions were increased in a dose- and time-dependent manner, whereas SAHH protein expressions were decreased...
May 9, 2018: Chemosphere
Hai-Rui Liu, Qing-Bo Gao, Fa-Qi Zhang, Gulzar Khan, Shi-Long Chen
The varying topography and environment that resulted from paleoorogeny and climate fluctuations of the Himalaya-Hengduan Mountains (HHM) areas had a considerable impact on the evolution of biota during the Quaternary. To understand the phylogeographic pattern and historical dynamics of Triosteum himalayanum (Caprifoliaceae), we sequenced three chloroplast DNA fragments ( rbcL-accD , rps15-ycf1 , and trnH-psbA ) from 238 individuals representing 20 populations. Nineteen haplotypes (H1-H19) were identified based on 23 single-site mutations and eight indels...
2018: PeerJ
Na Zhang, Tingting Geng, Zhangsheng Wang, Ruling Zhang, Tiefeng Cao, Joao Paulo Camporez, Shi-Ying Cai, Ya Liu, Luisa Dandolo, Gerald I Shulman, Gordon G Carmichael, Hugh S Taylor, Yingqun Huang
Excessive hepatic glucose production (HGP) contributes significantly to the hyperglycemia of type 2 diabetes; however, the molecular mechanism underlying this dysregulation remains poorly understood. Here, we show that fasting temporally increases the expression of H19 long noncoding RNA (lncRNA) in nondiabetic mouse liver, whereas its level is chronically elevated in diet-induced diabetic mice, consistent with the previously reported chronic hepatic H19 increase in diabetic patients. Importantly, liver-specific H19 overexpression promotes HGP, hyperglycemia, and insulin resistance, while H19 depletion enhances insulin-dependent suppression of HGP...
May 17, 2018: JCI Insight
Min Yang, Iuri Perisse, Zhiqiang Fan, Misha Regouski, Mirella Meyer-Ficca, Irina A Polejaeva
Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of precious transgenic lines or resetting the lifespan of primary transgenic cells for multiple genetic modifications. We successfully produced second-generation cloned goats using donor neonatal fibroblasts from first-generation clones. However, our attempts to produce any third-generation clones failed. SCNT efficiency decreased progressively with the clonal generations. The rate of pregnancy loss was significantly greater in recloning groups (P<0...
May 17, 2018: Reproduction, Fertility, and Development
Xiangning Bai, Sara Mernelius, Cecilia Jernberg, Ing-Marie Einemo, Stefan Monecke, Ralf Ehricht, Sture Löfgren, Andreas Matussek
Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhea (BD), hemorrhagic colitis (HC), and even hemolytic uremic syndrome (HUS). In Nordic countries, STEC are widely spread and usually associated with gastrointestinal symptoms and HUS. The objective of this study was to investigate the occurrence of STEC in Swedish patients over 10 years of age from 2003 through 2015, and to analyze the correlation of critical STEC virulence factors with clinical symptoms and duration of stx shedding. Diarrheal stool samples were screened for presence of stx by real-time PCR...
2018: Frontiers in Cellular and Infection Microbiology
Ercan Aydemir, Sevgi Kansiz, Mustafa Kemal Gumus, Nikolay Yu Gorobets, Necmi Dege
The title compound, C19 H19 N5 O2 , was prepared by the reaction of 3-amino-5-(pyridin-3-yl)-1,2,4-triazole with acetone and 2-hy-droxy-3-eth-oxy-benzaldehyde. It crystallizes from ethanol in a tetra-gonal space group, with one mol-ecule in the asymmetric unit. The 1,2,4-triazole five-membered ring is planar (maximum deviation = 0.0028 Å). The pyridine and phenyl rings are also planar with maximum deviations of 0.0091 and 0.0094 Å, respectively. In the crystal, N-H⋯N hydrogen bonds link the mol-ecules into supra-molecular chains propagating along the c- axis direction...
March 1, 2018: Acta Crystallographica. Section E, Crystallographic Communications
Hemant P Yennawar, Michaela J Buchwalter, Baylee K Colburn, Lee J Silverberg
The syntheses and crystal structures of 2,3-bis-[3-(tri-fluoro-meth-yl)phen-yl]-2,3-di-hydro-4 H -1,3-benzo-thia-zin-4-one (di- m -CF3 ; C22 H13 F6 NOS) ( 1 ) and 2,3-bis-(4-methyl-phen-yl)-2,3-di-hydro-4 H -1,3-benzo-thia-zin-4-one (di- p -CH3 ; C22 H19 NOS) ( 2 ) are reported. Each structure is racemic: the asymmetric unit of 2 consists of two mol-ecules. In both 1 and 2 , the six-membered 1,3-thia-zine ring is close to an envelope conformation with the chiral C atom forming the flap. In 1 , the 2-aryl group is pseudo-equatorial, while in 2 it is pseudo-axial...
March 1, 2018: Acta Crystallographica. Section E, Crystallographic Communications
Heidi Marjonen, Mia Toivonen, Laura Lahti, Nina Kaminen-Ahola
Prenatal alcohol exposure (PAE) can harm the embryonic development and cause life-long consequences in offspring's health. To clarify the molecular mechanisms of PAE we have used a mouse model of early alcohol exposure, which is based on maternal ad libitum ingestion of 10% (v/v) ethanol for the first eight days of gestation (GD 0.5-8.5). Owing to the detected postnatal growth-restricted phenotype in the offspring of this mouse model and both prenatal and postnatal growth restriction in alcohol-exposed humans, we focused on imprinted genes Insulin-like growth factor 2 (Igf2), H19, Small Nuclear Ribonucleoprotein Polypeptide N (Snrpn) and Paternally expressed gene 3 (Peg3), which all are known to be involved in embryonic and placental growth and development...
2018: PloS One
Q-P Zhou, F Zhang, J Zhang, D Ma
OBJECTIVE: We explored the possible mechanism underlying the expression change of H19 during fracture healing. MATERIALS AND METHODS: A total of 18 male SD mice aged from 6-8 weeks old (18.5-24.6 g) were selected to establish tibial fracture models. The left tibia undergoing sham surgery was considered as the control group, and the right tibia undergoing sawing treatment was considered as the experimental group. The control tibia and fracture tibia from three mice were harvested at six time points after operation, respectively...
April 2018: European Review for Medical and Pharmacological Sciences
Varune Rohan Ramnarine, Mohammed Alshalalfa, Fan Mo, Noushin Nabavi, Nicholas Erho, Mandeep Takhar, Robert Shukin, Sonal Brahmbhatt, Alexander Gawronski, Maxim Kobelev, Mannan Nouri, Dong Lin, Harrison Tsai, Tamara L Lotan, R Jefferey Karnes, Mark A Rubin, Amina Zoubeidi, Martin E Gleave, Cenk Sahinalp, Alexander W Wyatt, Stanislav V Volik, Himisha Beltran, Elai Davicioni, Yuzhuo Wang, Colin C Collins
Background: Treatment induced neuroendocrine prostate cancer (tNEPC) is an aggressive variant of late-stage metastatic castrate resistant (mCRPC) prostate cancer that commonly arises through neuroendocrine transdifferentiation (NEtD). Treatment options are limited, ineffective, and for most patients, results in death in less than a year. We previously developed a first-in-field patient-derived xenograft (PDX) model of NEtD. Longitudinal deep transcriptome profiling of this model enabled monitoring of dynamic transcriptional changes during NEtD and in the context of androgen deprivation...
May 10, 2018: GigaScience
Dayong Ding, Changfeng Li, Tiancheng Zhao, Dandan Li, Lei Yang, Bin Zhang
This investigation was aimed at working out the combined role of lncRNA H19, miR-29b and Wnt signaling in the development of colorectal cancer (CRC). In the aggregate, 185 CRC tissues and corresponding para-carcinoma tissues were gathered. The human CRC cell lines (i.e. HT29, HCT116, SW480 and SW620) and normal colorectal mucosa cell line (NCM460) were also purchased. Si-H19, si-NC, miR-29b-3p mimics, miR-29b-3p inhibitor, si-PGRN and negative control (NC) were, respectively, transfected into the CRC cells...
May 10, 2018: Molecules and Cells
Ping Cui, Yanrui Zhao, Xinlei Chu, Na He, Hong Zheng, Jiali Han, Fengju Song, Kexin Chen
PURPOSE: An increasing number of long intergenic non-coding RNAs (lincRNAs) appear to play critical roles in cancer development and progression. To assess the association between SNPs that reside in regions of lincRNAs and breast cancer risk, we performed a large case-control study in China. METHODS: We carried out a two-stage case-control study including 2881 breast cancer cases and 3220 controls. In stage I, we genotyped 17 independent (r2  < 0.5) SNPs located in 6 tumor-related lincRNAs by using the TaqMan platform...
May 8, 2018: Breast Cancer Research and Treatment
S S Sahraei, M Shahhoseini, B Movaghar
BACKGROUND: OBJECTIVE: This paper reports results from studies on modifications of histone marks in transcriptional regulatory regions of H19, Igf2 and Mest genes in vitrified-warmed and cultured mouse embryos. MATERIALS AND METHODS: Non vitrified and vitrified-warmed 8-cell embryos were cultured in Ham's F10+10% BSA into blastocyst stage in two experimental groups. Gene expression level was assessed by quantitative PCR and histone modifications were evaluated by ChIP assay method...
March 2018: Cryo Letters
Wan-Wei Wang, Xi-Lei Zhou, Ying-Jian Song, Chang-Hua Yu, Wei-Guo Zhu, Yu-Suo Tong
Purpose: Long noncoding RNAs (lncRNAs) are present in body fluids, but their potential as tumor biomarkers has never been investigated in malignant pleural effusion (MPE) caused by lung cancer. The aim of this study was to assess the clinical significance of lncRNAs in pleural effusion, which could potentially serve as diagnostic and predictive markers for lung cancer-associated MPE (LC-MPE). Patients and methods: RNAs from pleural effusion were extracted in 217 cases of LC-MPE and 132 cases of benign pleural effusion (BPE)...
2018: OncoTargets and Therapy
Marta Otręba, Daria Budzikur, Łukasz Górecki, Katarzyna A Řlepokura
Adenosine diphosphate (ADP) plays a crucial role in cell biochemistry, especially in metabolic pathways and energy storage. ADP itself, as well as many of its analogues, such as adenosine hypodiphosphate (AhDP), has been studied extensively, in particular in terms of enzymatic activity. However, structural studies in the solid state, especially for AhDP, are still missing. An analogue of ADP, i.e. adenosine hypodiphosphate ester, has been synthesized and characterized in the crystalline form as two hydrated sodium salts of 2':3'-isopropylideneadenosine 5'-hypodiphosphate (H3 AhDP, C13 H19 N5 O9 P2 for the neutral form), namely pentasodium tetrakis(2':3'-isopropylideneadenosine 5'-hypodiphosphate) tetracosahydrate, 5Na+ ·3C13 H18 N5 O9 P2 - ·C13 H17 N5 O9 P2 2- ·24H2 O or Na5 (H2 AhDP)3 (HAhDP)·24H2 O, (I), and sodium tetrakis(2':3'-isopropylideneadenosine 5'-hypodiphosphate) pentadecahydrate, Na+ ·C13 H20 N5 O9 P2 + ·2C13 H18 N5 O9 P2 - ·C13 H19 N5 O9 P2 ·15H2 O or Na(H4 AhDP)(H3 AhDP)(H2 AhDP)2 ·15H2 O, (II)...
May 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
Jin-Liang Zhu, Yin-Ling Wu, Wen-Hao Tang, Yuan Tian, Shao-Qin Ge, Ping Liu, Jie Qiao
Objective: To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA. METHODS: We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO. RESULTS: No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93...
June 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
Kanagaraj Arun, Ganesan Arunkumar, Duraisamy Bennet, Servarayan Murugesan Chandramohan, Avaniyapuram Kannan Murugan, Arasambattu Kannan Munirajan
Gastric cancer remains fifth most common cancer often diagnosed at an advanced stage and is the second leading cause of cancer-related death worldwide. Long non-coding RNAs (lncRNAs) involved in various cellular pathways are essential for tumor occurrence and progression and they have high potential to promote or suppress the expression of many genes. In this study, we profiled 19 selected cancer-associated lncRNAs in thirty gastric adenocarcinomas and matching normal tissues by qRT-PCR. Our results showed that most of the lncRNAs were significantly upregulated (12/19)...
April 6, 2018: Oncotarget
Hongli Peng, Panlin Zhao, Jiaonan Liu, Jinxia Zhang, Jie Zhang, Youxin Wang, Lijuan Wu, Manshu Song, Wei Wang
Male infertility is an important global health burden that can benefit from novel biomarkers and diagnostics innovation. Aberrant methylation of the imprinted genes H19 and SNRPN (small nuclear ribonucleoprotein polypeptide N) in sperm DNA has been implicated in abnormal sperm parameters and male infertility. However, whether certain methylation patterns of one or multiple CpG sites within an imprinted gene are pathological for multiple sperm defects remains poorly understood. To examine the diagnostic potential of certain methylation patterns of CpG sites for multiphenotype defects in human sperm, the sperm DNA methylation patterns of individual CpG sites within imprinting control regions (ICRs) of imprinted genes H19 and SNRPN were measured by bisulfite pyrosequencing in a Han Chinese population sample: 39 oligoasthenozoospermia (OA) patients, 36 asthenoteratozoospermia (AT) patients, and 50 normozoospermia (N) controls...
April 30, 2018: Omics: a Journal of Integrative Biology
Joëlle El Hajj, Eric Nguyen, Qingyuan Liu, Claire Bouyer, Eric Adriaenssens, George Hilal, Evelyne Ségal-Bendirdjian
BACKGROUND: Since tumor growth requires reactivation of telomerase (hTERT), this enzyme is a challenging target for drug development. Therefore, it is of great interest to identify telomerase expression and activity regulators. Retinoids are well-known inducers of granulocytic maturation associated with hTERT repression in acute promyelocytic leukemia (APL) blasts. In a maturation-resistant APL cell line, we have previously identified a new pathway of retinoid-induced hTERT transcriptional repression independent of differentiation...
April 27, 2018: Molecular Cancer
Zhaoxu Lu, Yufeng Ma, Linying Gao, Yingjun Li, Qiang Li, Mei Qiang
BACKGROUND: Mercury (Hg) is a well-recognized environmental pollutant known by its toxicity of development and neurotoxicity, which results in adverse health outcomes. However, the mechanisms underlying the teratogenic effects of Hg are not well understood. Imprinting genes are emerging regulators for fetal development subjecting to environmental pollutants impacts. In this study, we examined the association between preconceptional Hg exposure and the alteration of DNA methylation of imprinting genes H19, Meg3, and Peg3 in human sperm DNA...
2018: PloS One
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