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M Di Sanzo, R Chirillo, I Aversa, F Biamonte, G Santamaria, E D Giovannone, M C Faniello, G Cuda, F Costanzo
PURPOSE: The heavy subunit of the iron storage protein ferritin (FHC) is essential for the intracellular iron metabolism and, at the same time, it represents a central hub of iron-independent pathways, such as cell proliferation, angiogenesis, p53 regulation, chemokine signalling, stem cell expansion, miRNAs expression. In this work we have explored the ability of FHC to modulate gene expression in K562 cells, through the up-regulation of the lncRNA H19 and its cognate miR-675. MATERIALS AND METHODS: Targeted silencing of FHC was performed by lentiviral-driven shRNA strategy...
March 12, 2018: Gene
Tingchao Mao, Chengquan Han, Ruizhi Deng, Biao Wei, Peng Meng, Yan Luo, Yong Zhang
Epigenetic modifications extensively occur in mammalian embryonic development and cell differentiation process. They play an essential role in the reprogramming of nuclei during somatic cell nuclear transfer (SCNT) and subsequent in vitro embryonic development. Recently, SCNT embryos have been verified to contain a subnormal level of histone H3K4 dimethylation (H3K4me2) in contrast to in vitro fertilized embryos. This finding suggested that increasing H3K4me2 levels may ameliorate the aberrant development of cloned embryos...
March 15, 2018: Systems Biology in Reproductive Medicine
Shuping Peng, Lihua Cao, Shiwei He, Yancheng Zhong, Haotian Ma, Yanru Zhang, Cijun Shuai
Bone regeneration is very important for the recovery of some diseases including osteoporosis and bone fracture trauma. It is a multiple-step- and multiple-gene-involved complex process, including the matrix secretion and calcium mineralization by osteoblasts differentiated from mesenchymal stem cells (MSCs) and the absorption of calcium and phosphorus by osteoclasts differentiated from hematopoietic stem cells. Long noncoding RNAs (lncRNAs) are a family of transcripts longer than 200 nt without or with very low protein-coding potential...
2018: Stem Cells International
Edward W Pietryk, Kiristin Clement, Marwa Elnagheeb, Ryan Kuster, Kayla Kilpatrick, Michael I Love, Folami Y Ideraabdullah
In utero exposure to vinclozolin (VIN), an antiandrogenic fungicide, is linked to multigenerational phenotypic and epigenetic effects. Mechanisms remain unclear. We assessed the role of antiandrogenic activity and DNA sequence context by comparing effects of VIN vs. M2 (metabolite with greater antiandrogenic activity) and wild-type C57BL/6 (B6) mice vs. mice carrying mutations at the previously reported VIN-responsive H19/Igf2 locus. First generation offspring from VIN-treated 8nrCG mutant dams exhibited increased body weight and decreased sperm ICR methylation...
March 10, 2018: Reproductive Toxicology
Ni Kou, Sha Liu, Xiaojie Li, Wuwei Li, Weijian Zhong, Lin Gui, Songling Chai, Xiang Ren, Risu Na, Tao Zeng, Huiying Liu
The long noncoding RNA (lncRNA) H19 has been described to participate in various tumors metastasis. Nevertheless, whether H19 promotes or impedestongue squamous cell carcinoma (TSCC) cells migration and invasion remains controversial. Here, we found that the expression of H19 was elevated in TSCC tissues compared with adjacent normal tissues. Moreover, we demonstrated that the expression of H19 was higher in metastasized tumors compared with un-metastasized tumors. Consistently, TSCC cells express higher levels of H19 than human squanous cells...
March 9, 2018: Oncology Research
Jin Xu, Yanqing Xia, Helong Zhang, Haibin Guo, Ke Feng, Cuilian Zhang
BACKGROUND: Preeclampsia (PE), characterized by hypertension and proteinuria, is a leading cause of perinatal and maternal mortality. Considering that mutation of H19 gene is closely associated with PE, we aimed to explore the functional role of long non-coding RNA H19 (lncRNA-H19) in trophoblast cells. METHODS: Expression of lncRNA-H19 in placenta tissues from patients with PE and healthy pregnant women after delivery was determined by quantitative reverse transcription PCR...
March 6, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Xiaobin Wang, Mingxiang Zou, Jing Li, Bing Wang, Qianshi Zhang, Fubin Liu, Guohua Lü
Intervertebral disc (IVD) degeneration (IDD) is a major contributor to low back pain. During IDD progression, ROS can be produced in the form of H2 O2 in nucleus pulposus cells (NPCs) in response to elevated cytokines, leading to subsequent alternations of cell fate and metabolic processes. Genetic factors are considered as main contributors to IDD pathopoiesis. Herein, we investigated the detailed function and mechanism of H19, one of the most up-regulated lncRNAs in IDD specimens, in H2 O2 -induced cell senescence model in NPCs...
March 9, 2018: Journal of Cellular Biochemistry
Sunghyuk Lim, Graham Roseman, Igor Peshenko, Grace Manchala, Diana Cudia, Alexander M Dizhoor, Glenn Millhauser, James B Ames
Retinal guanylyl cyclases (RetGCs) in vertebrate photoreceptors are regulated by the guanylyl cyclase activator proteins (GCAP1 and GCAP2). Here, we report EPR double electron-electron resonance (DEER) studies on the most ubiquitous GCAP isoform, GCAP1 and site-directed mutagenesis analysis to determine an atomic resolution structural model of a GCAP1 dimer. Nitroxide spin-label probes were introduced at individual GCAP1 residues: T29C, E57C, E133C, and E154C. The intermolecular distance of each spin-label probe (measured by DEER) defined restraints for calculating the GCAP1 dimeric structure by molecular docking...
2018: PloS One
L Montrose, V Padmanabhan, J M Goodrich, S E Domino, M C Treadwell, J D Meeker, D J Watkins, D C Dolinoy
Endocrine disrupting chemicals (EDCs) pose a public health risk through disruption of normal biological processes. Identifying toxicoepigenetic mechanisms of developmental exposure-induced effects for EDCs, such as phthalates or bisphenol A (BPA), is essential. Here, we investigate whether maternal exposure to EDCs is predictive of infant DNA methylation at candidate gene regions. In the Michigan Mother-Infant Pairs (MMIP) cohort, DNA was extracted from cord blood leukocytes for methylation analysis by pyrosequencing (n = 116) and methylation changes related to first trimester levels of 9 phthalate metabolites and BPA...
March 7, 2018: Epigenetics: Official Journal of the DNA Methylation Society
Asghar Farzi-Molan, Sadegh Babashah, Babak Bakhshinejad, Amir Atashi, Masoumeh Fakhr-Taha
The differentiation of human bone marrow mesenchymal stem cells (BMSCs) into specific lineages offers new opportunities to use the therapeutic efficiency of these pluripotent cells in regenerative medicine. Multiple lines of evidence have revealed that non-coding RNAs play major roles in the differentiation of BMSCs into neural cells. Here, we applied a cocktail of neural inducing factors (NIFs) to differentiate BMSCs into neural-like cells. Our data demonstrated that during neurogenic induction, BMSCs obtained a neuron-like morphology...
March 7, 2018: Cell Biology International
Ming-Li Yang, Zhe Huang, Qian Wang, Huan-Huan Chen, Sai-Nan Ma, Rong Wu, Wei-Song Cai
Background: Hepatocellular cancer (HCC) is one of the major causes of cancer related mortality. Genetic polymorphisms may affect the susceptibility and clinical outcome of cancers. We aim to manifest the association of single nucleotide polymorphisms (SNPs) of lncRNA- H19 gene with the risk and prognosis of HCC. Methods: A total of 944 samples composed of 472 HCC patients and 472 matched controls were included in the risk analysis and among them 350 HCC samples were investigated in the prognosis analysis. KASP method was conducted for the SNP genotyping...
March 6, 2018: Bioscience Reports
Lina M Acosta Quintero, Isidro Burgos, Alirio Palma, Justo Cobo, Christopher Glidewell
A concise, efficient and versatile synthesis of amino-substituted benzo[b]pyrimido[5,4-f]azepines is described: starting from a 5-allyl-4,6-dichloropyrimidine, the synthesis involves base-catalysed aminolysis followed by intramolecular Friedel-Crafts cyclization. Four new amino-substituted benzo[b]pyrimido[5,4-f]azepines are reported, and all the products and reaction intermediates have been fully characterized by IR,1 H and13 C NMR spectroscopy and mass spectrometry, and the molecular and supramolecular structures of three products and one intermediate have been determined...
March 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
Sarah Gonzalez-Nahm, Michelle Mendez, Whitney Robinson, Susan K Murphy, Cathrine Hoyo, Vijaya Hogan, Diane Rowley
Diet is dictated by the surrounding environment, as food access and availability may change depending on where one lives. Maternal diet during pregnancy is an important part of the in utero environment, and may affect the epigenome. Studies looking at overall diet pattern in relation to DNA methylation have been lacking. The Mediterranean diet is known for its health benefits, including decreased inflammation, weight loss, and management of chronic diseases. This study assesses the association between maternal adherence to a Mediterranean diet pattern during pregnancy and infant DNA methylation at birth...
May 2017: Environmental Epigenetics
Angela Sparago, Flavia Cerrato, Andrea Riccio
Background: Loss of paternal methylation (LOM) of the H19/IGF2 intergenic differentially methylated region ( H19/IGF2 :IG-DMR) causes alteration of H19/IGF2 imprinting and Silver-Russell syndrome (SRS). Recently, internal deletions of the H19/IGF2 :IG-DMR have been associated with LOM and SRS when present on the paternal chromosome. In contrast, previously described deletions, most of which cause gain of methylation (GOM) and Beckwith-Wiedemann syndrome (BWS) on maternal transmission, were consistently associated with normal methylation and phenotype if paternally inherited...
2018: Clinical Epigenetics
Wenyuan Wang, Qin Yang, Qiaojia Huang, Hua Zhang, Zaizhong Zhang, Jian Gao, Wenjun Ren, Yongli Hu, Youdong Lin, Yuan Dang, Fan Zhang, Wen Wang, Lie Wang
BACKGROUND: It has been widely demonstrated that long non-coding RNA H19 (lncRNA H19) plays an important role in the progression of various human cancers. However, the associations of common genetic variations with recurrence and survival in gastric adenocarcinoma in this lncRNA remain largely unknown. METHODS: The rs2839698 single nucleotide polymorphism (SNP) of H19 was genotyped in tissue samples from 441 patients with T3 gastric adenocarcinoma who had surgical operations between 2004 to 2009, and the relationships between the different genotypes and recurrence and survival after surgery alone (n = 156) or surgery plus chemotherapy (n = 285) were assessed using 3 different statistical-methods...
January 1, 2018: Clinical Laboratory
Oswaldo Gomes Júnior, Valdislaine M Silva, Antonio E H Machado, Carla Sirtori, Camila R Lemos, Adriane M Freitas, Alam G Trovó
Ciprofloxacin has been determined with high frequency in studies involving environmental waters matrixes. However, no study evaluating the correlation between the initial pH and molar iron/organic ligand ratio has been published. This paper describes the degradation of the antibiotic ciprofloxacin by the photo-Fenton process using different sources of iron (Fe2+ , Fe3+ and Fe3+ -citrate and Fe3+ -oxalate, named FeCit and FeOx, respectively) and molar iron/organic ligand ratios at initial pH values of 2.5 and 6...
February 22, 2018: Journal of Environmental Management
Yafang Pan, Hongmei Chen, Xianjuan Shen, Xudong Wang, Shaoqing Ju, Meihong Lu, Hui Cong
Circulating long noncoding RNA (lncRNA) H19 has been reported to be a biomarker for cancer monitoring. The purpose of this study was to determine whether serum lncRNA could serve as a novel biomarker for the diagnosis of multiple myeloma (MM) and evaluate its value of clinical application. In our study, the expression of lncRNA H19 was up-regulated in 80 patients with MM and MM cell lines by RT-PCR analysis. Clinicopathological analysis showed the expression level of H19 could assist clinical staging, and the severity of the disease could be roughly determined according to the amount of H19 expressed in the patient serum...
February 19, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Andrea Freschi, Stella K Hur, Federica Maria Valente, Folami Y Ideraabdullah, Angela Sparago, Maria Teresa Gentile, Andrea Oneglia, Diego Di Nucci, Luca Colucci-D'Amato, Joanne L Thorvaldsen, Marisa S Bartolomei, Andrea Riccio, Flavia Cerrato
Differential DNA methylation defects of H19/IGF2 are associated with congenital growth disorders characterized by opposite clinical pictures. Due to structural differences between human and mouse, the mechanisms by which mutations of the H19/IGF2 Imprinting Control region (IC1) result in these diseases are undefined. To address this issue, we previously generated a mouse line carrying a humanized IC1 (hIC1) and now replaced the wildtype with a mutant IC1 identified in the overgrowth-associated Beckwith-Wiedemann syndrome...
February 22, 2018: PLoS Genetics
Zahre Sajadpoor, Zeinab Amini-Farsani, Hossein Teimori, Mehdi Shamsara, Mohammad Hossein Sangtarash, Payam Ghasemi-Dehkordi, Farrokh Yadollahi
Cisplatin resistance is one of the main limitations in the treatment of ovarian cancer, which is partly mediated by long noncoding RNAs (lncRNAs). H19 is a lncRNA involving in cisplatin resistance in cancers. Valproic acid (VPA) is a commonly used drug for clinical treatment of seizure disorders. In addition, this drug may display its effects through regulation of noncoding RNAs controlling gene expression. The aim of the present study was the investigation of VPA treatment effect on H19 expression in ovarian cancer cells and also the relation of the H19 levels with apoptosis and cisplatin resistance...
February 22, 2018: Applied Biochemistry and Biotechnology
Jing Yang, Xiaojun Shao, Jian Jiang, Yan Sun, Lingzhen Wang, Lirong Sun
Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Effects of AP on cell viability, proliferation, apoptosis, migration, invasion and expressions of long noncoding RNA H19 (lncRNA-H19), microRNA (miR)-675 and CD44 were assessed...
February 21, 2018: Cell Biology International
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