Bingchen Che, Dan Sun, Chen Zhang, Jiaqing Hou, Wei Zhao, Guangyin Jing, Yuguang Mu, Yaoyu Cao, Liang Dai, Ce Zhang
Mechanically induced chromosome reorganization plays important roles in transcriptional regulation. However, the interplay between chromosome reorganization and transcription activities is complicated, such that it is difficult to decipher the regulatory effects of intranuclear geometrical cues. Here, we simplify the system by introducing DNA, packaging proteins (i.e., histone and protamine), and transcription factor NF-κB into a well-defined fluidic chip with changing spatical confinement ranging from 100 to 500 nm...
March 1, 2023: Nano Letters