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oxidative stress and the diaphragm

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https://www.readbyqxmd.com/read/29893682/immunohistochemical-analysis-of-the-spinal-cord-ischemia-effect-of-remote-ischemic-preconditioning-in-a-porcine-model
#1
Henri Johannes Haapanen, Johanna Herajärvi, Hannu-Pekka Honkanen, Caius Mustonen, Hannu Tuominen, Ulla Puistola, Vesa Anttila, Tatu Juvonen
In experimental settings, remote ischemic preconditioning (RIPC) has shown a positive effect regarding spinal cord protection after local ischemia. In this study, we conducted spinal cord immunohistochemistry to demonstrate the protective effect of RIPC after 24 hours of the regional ischemia. Methods: Twenty piglets were randomized into an RIPC group (n = 10) and a control group (n = 10). The RIPC group underwent transient left hind limb ischemia before systematic left subclavian artery and segmental artery occlusion at the level of the diaphragm...
May 29, 2018: Heart Surgery Forum
https://www.readbyqxmd.com/read/29843457/taurine-supplementation-alleviates-puromycin-aminonucleoside-damage-by-modulating-endoplasmic-reticulum-stress-and-mitochondrial-related-apoptosis-in-rat-kidney
#2
Alessandra Stacchiotti, Gaia Favero, Antonio Lavazza, Maria Monsalve, Luigi Fabrizio Rodella, Rita Rezzani
Taurine (TAU) is a sulfur-containing beta amino acid that is not involved in protein composition and anabolism, conditionally essential in mammals provided through diet. Growing evidence supports a protective role of TAU supply in osmoregulation, calcium flux, and reduction of inflammation and oxidant damage in renal diseases like diabetes. Endoplasmic reticulum (ER) stress, due to abnormal proteostasis, is a contributor to nephrotic syndrome and related renal damage. Here, we investigated the effect of dietary TAU (1...
May 29, 2018: Nutrients
https://www.readbyqxmd.com/read/29768017/the-signaling-network-resulting-in-ventilator-induced-diaphragm-dysfunction
#3
Huibin Tang, Joseph B Shrager
Mechanical ventilation (MV) is a life-saving measure for those incapable of adequately ventilating or oxygenating without assistance. Unfortunately, even brief periods of MV result in diaphragm weakness (i.e., "ventilator-induced diaphragm dysfunction" - VIDD) that may render it difficult to wean the ventilator. Prolonged MV is associated with cascading complications and is a strong risk factor for death. Thus, prevention of VIDD may have a dramatic impact on mortality rates. Here, we summarized the current understanding of the pathogenic events underlying VIDD...
May 16, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29670681/diaphragm-muscle-weakness-following-acute-sustained-hypoxic-stress-in-the-mouse-is-prevented-by-pretreatment-with-n-acetyl-cysteine
#4
Andrew J O'Leary, Sarah E Drummond, Deirdre Edge, Ken D O'Halloran
Oxygen deficit (hypoxia) is a major feature of cardiorespiratory diseases characterized by diaphragm dysfunction, yet the putative role of hypoxic stress as a driver of diaphragm dysfunction is understudied. We explored the cellular and functional consequences of sustained hypoxic stress in a mouse model. Adult male mice were exposed to 8 hours of normoxia, or hypoxia (FiO2  = 0.10) with or without antioxidant pretreatment (N-acetyl cysteine, 200 mg/kg i.p.). Ventilation and metabolism were measured. Diaphragm muscle contractile function, myofibre size and distribution, gene expression, protein signalling cascades, and oxidative stress (TBARS) were determined...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29376467/diaphragmatic-dysfunction-in-sepsis-due-to-severe-acute-pancreatitis-complicated-by-intra-abdominal-hypertension
#5
Wei-Chao Liao, Yan-Hong Chen, Hang-Yang Li, Ting-Ting Wang, Peng Lan, Kong-Han Pan, Hui-Qing Ge, Qiang-Min Xie, Jian-Cang Zhou
Objective This study aimed to examine the mechanism of diaphragmatic dysfunction in sepsis due to severe acute pancreatitis (SAP) with intra-abdominal hypertension (IAH) in a rat model. Methods The rats were assigned at random to four groups: (1) control (n = 5), (2) SAP (n = 5), (3) SAP+IAH (n = 5), and (4) SAP+IAH+SS-31 (n = 5). Length and force output of the diaphragm were analysed in vivo. Histopathological examinations were performed by haematoxylin-eosin. Oxidative stress levels related to protease in diaphragmatic mitochondria were detected with a colorimetric technique...
April 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29346133/dexmedetomidine-impairs-diaphragm-function-and-increases-oxidative-stress-but-does-not-aggravate-diaphragmatic-atrophy-in-mechanically-ventilated-rats
#6
Thomas Breuer, Christian Bleilevens, Rolf Rossaint, Gernot Marx, Julian Gehrenkemper, Henning Dierksen, Antoine Delpierre, Joachim Weis, Ghislaine Gayan-Ramirez, Christian S Bruells
BACKGROUND: Anesthetics in ventilated patients are critical as any cofactor hampering diaphragmatic function may have a negative impact on the weaning progress and therefore on patients' mortality. Dexmedetomidine may display antioxidant and antiproteolytic properties, but it also reduced glucose uptake by the muscle, which may impair diaphragm force production. This study tested the hypothesis that dexmedetomidine could inhibit ventilator-induced diaphragmatic dysfunction. METHODS: Twenty-four rats were separated into three groups (n = 8/group)...
April 2018: Anesthesiology
https://www.readbyqxmd.com/read/29230271/the-role-of-oxidative-stress-in-decreased-acetylcholinesterase-activity-at-the-neuromuscular-junction-of-the-diaphragm-during-sepsis
#7
Hua Liu, Jin Wu, Jun-Yan Yao, Hong Wang, Shi-Tong Li
Our recent study demonstrated that acetylcholinesterase (AChE) activity at the neuromuscular junction (NMJ) of the diaphragm decreased during sepsis. However, the mechanisms were not clearly identified. In this study, we aimed to investigate whether the decreased AChE activity was related to oxidative stress by observing AChE activity in different grades of sepsis induced by caecal ligation and puncture (CLP). At 24 h after surgery, an assay of thiobarbituric acid reactive species (TBARS) and protein carbonyls, as well as the myeloperoxidase (MPO), superoxide dismutase (SOD), and catalase (CAT) activity, was conducted...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29212808/gestational-age-at-time-of-in-utero-lipopolysaccharide-exposure-influences-the-severity-of-inflammation-induced-diaphragm-weakness-in-lambs
#8
Kanakeswary Karisnan, Tanzila Mahzabin, Anthony J Bakker, Yong Song, Peter B Noble, J Jane Pillow, Gavin J Pinniger
The preterm diaphragm is functionally immature compared with its term counterpart. In utero inflammation further exacerbates preterm diaphragm dysfunction. We hypothesized that preterm lambs are more vulnerable to in utero inflammation-induced diaphragm dysfunction compared with term lambs. Pregnant ewes received intra-amniotic (IA) injections of saline or 10 mg lipopolysaccharide (LPS) 2 or 7 days before delivery at 121 days (preterm) or ∼145 days (term) of gestation. Diaphragm contractile function was assessed in vitro...
April 1, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29210997/tempol-supplementation-restores-diaphragm-force-and-metabolic-enzyme-activities-in-mdx-mice
#9
David P Burns, Izza Ali, Clement Rieux, James Healy, Greg Jasionek, Ken D O'Halloran
Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of dystrophin-deficient diaphragm muscle. Diaphragm muscle function was assessed, ex vivo, in adult male wild-type and dystrophin-deficient mdx mice, with and without a 14-day antioxidant intervention...
December 6, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29205354/podocytes-are-new-cellular-targets-of-haemoglobin-mediated-renal-damage
#10
Alfonso Rubio-Navarro, Maria Dolores Sanchez-Niño, Melania Guerrero-Hue, Cristina García-Caballero, Eduardo Gutiérrez, Claudia Yuste, Ángel Sevillano, Manuel Praga, Javier Egea, Elena Román, Pablo Cannata, Rosa Ortega, Isabel Cortegano, Belén de Andrés, María Luisa Gaspar, Susana Cadenas, Alberto Ortiz, Jesús Egido, Juan Antonio Moreno
Recurrent and massive intravascular haemolysis induces proteinuria, glomerulosclerosis, and progressive impairment of renal function, suggesting podocyte injury. However, the effects of haemoglobin (Hb) on podocytes remain unexplored. Our results show that cultured human podocytes or podocytes isolated from murine glomeruli bound and endocytosed Hb through the megalin-cubilin receptor system, thus resulting in increased intracellular Hb catabolism, oxidative stress, activation of the intrinsic apoptosis pathway, and altered podocyte morphology, with decreased expression of the slit diaphragm proteins nephrin and synaptopodin...
March 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29197632/crosstalk-between-autophagy-and-oxidative-stress-regulates-proteolysis-in-the-diaphragm-during-mechanical-ventilation
#11
Ashley J Smuder, Kurt J Sollanek, W Bradley Nelson, Kisuk Min, Erin E Talbert, Andreas N Kavazis, Matthew B Hudson, Marco Sandri, Hazel H Szeto, Scott K Powers
Mechanical ventilation (MV) results in the rapid development of ventilator-induced diaphragm dysfunction (VIDD). While the mechanisms responsible for VIDD are not fully understood, recent data reveal that prolonged MV activates autophagy in the diaphragm, which may occur as a result of increased cellular reactive oxygen species (ROS) production. Therefore, we tested the hypothesis that (1) accelerated autophagy is a key contributor to VIDD; and that (2) oxidative stress is required to increase the expression of autophagy genes in the diaphragm...
February 1, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29109401/smad3-initiates-oxidative-stress-and-proteolysis-that-underlies-diaphragm-dysfunction-during-mechanical-ventilation
#12
Huibin Tang, Catherine L Kennedy, Myung Lee, Yang Gao, Hui Xia, Francesca Olguin, Danielle A Fraga, Kelsey Ayers, Sehoon Choi, Michael Kim, Amir Tehrani, Yasser A Sowb, Thomas A Rando, Joseph B Shrager
Prolonged use of mechanical ventilation (MV) leads to atrophy and dysfunction of the major inspiratory muscle, the diaphragm, contributing to ventilator dependence. Numerous studies have shown that proteolysis and oxidative stress are among the major effectors of ventilator-induced diaphragm muscle dysfunction (VIDD), but the upstream initiator(s) of this process remain to be elucidated. We report here that periodic diaphragm contraction via phrenic nerve stimulation (PNS) substantially reduces MV-induced proteolytic activity and oxidative stress in the diaphragm...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29072171/effects-of-dietary-omega-3-on-dystrophic-cardiac-and-diaphragm-muscles-as-evaluated-by-1-h-magnetic-resonance-spectroscopy-metabolic-profile-and-calcium-related-proteins
#13
Adriana Fogagnolo Maurício, Samara Camaçari de Carvalho, Humberto Santo Neto, Maria Julia Marques
BACKGROUND & AIMS: Duchenne muscular dystrophy (DMD) is characterized by the absence of dystrophin and muscle degeneration. Calcium dysregulation and oxidative stress also contribute to the disease progression. We evaluated the potential therapeutic benefits of supplementation with omega-3 on the metabolic profile, calcium-related proteins and oxidative stress response in the heart and diaphragm (DIA) of the mdx mouse model of DMD at later stages of the disease (13 months). METHODS: Mdx mice (8 months old) received omega-3 via a dietary supplement for 5 months...
August 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/28935143/vitamin-e-treatment-decreases-muscle-injury-in-mdx-mice
#14
Rafael Dias Mâncio, Túlio de Almeida Hermes, Aline Barbosa Macedo, Daniela Sayuri Mizobuti, Amanda Harduim Valduga, Ian Feller Rupcic, Elaine Minatel
OBJECTIVE: Oxidative stress, in addition to the absence of the dystrophin protein, has been considered an important regulator of Duchenne muscular dystrophy (DMD). Vitamin E presents an important role as a potent antioxidant and in preserving the integrity of the cell membrane. In this study, we evaluated the effects of vitamin E therapy on some physiological pathways that can contribute to muscle injury in the diaphragm muscle of mdx mice (the experimental model of DMD) such as CK levels, inflammatory response, oxidative stress, and the enzymatic antioxidant system...
November 2017: Nutrition
https://www.readbyqxmd.com/read/28790924/effects-of-ionizing-irradiation-on-mouse-diaphragmatic-skeletal-muscle
#15
Tingyang Zhou, Lanchun Lu, Shiyong Wu, Li Zuo
Undesirable exposure of diaphragm to radiation during thoracic radiation therapy has not been fully considered over the past decades. Our study aims to examine the potential biological effects on diaphragm induced by radiation. One-time ionizing irradiation of 10 Gy was applied either to the diaphragmatic region of mice or to the cultured C2C12 myocytes. Each sample was then assayed for muscle function, oxidative stress, or cell viability on days 1, 3, 5, and 7 after irradiation. Our mouse model shows that radiation significantly reduced muscle function on the 5th and 7th days and increased reactive oxygen species (ROS) formation in the diaphragm tissue from days 3 to 7...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28763042/optimization-of-a-voc-sensor-with-a-bilayered-diaphragm-using-fbar-as-strain-sensing-elements
#16
Huihui Guo, Aohui Guo, Yang Gao, Tingting Liu
Film bulk acoustic resonators (FBARs) are widely applied in mass bio-sensing and pressure sensors, owing to their extreme sensitivity and integration ability, and ability to miniaturize circuits. A volatile organic compound (VOC) sensor with a polymer-coated diaphragm, using FBARs as a strain sensing element is proposed and optimized. This vapor sensor is based on organic vapor-induced changes of mechanical deformation of the micro-diaphragm. The four FBARs are located at the edge of the bi-layer diaphragm comprising silicon nitride and silicon oxide for strain extraction...
August 1, 2017: Sensors
https://www.readbyqxmd.com/read/28719912/crocin-protects-podocytes-against-oxidative-stress-and-inflammation-induced-by-high-glucose-through-inhibition-of-nf-%C3%AE%C2%BAb
#17
Sutong Li, Xiaoxia Liu, Jie Lei, Junle Yang, Puxun Tian, Yi Gao
BACKGROUND/AIMS: Diabetic nephropathy (DN) is a microangiopathic disease characterized by excessive urinary albumin excretion, which occurs in 30% of patients with diabetes mellitus. It is the second leading cause of end-stage renal diseases in China. Nuclear factor-kappa B (NF-κB) is reported to be closely correlated with the inflammation underlying diabetes-associated renal damage. Crocin, a plant-derived compound, has antioxidant properties that may inhibit NF-κB. METHODS: In the present study, we used a conditionally immortalized mouse podocyte cell line to explore whether crocin could effectively block albuminuria...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28428331/a-personalized-model-of-coq2-nephropathy-rescued-by-the-wild-type-coq2-allele-or-dietary-coenzyme-q-10-supplementation
#18
Jun-Yi Zhu, Yulong Fu, Adam Richman, Zhanzheng Zhao, Patricio E Ray, Zhe Han
Clinical studies have identified patients with nephrotic syndrome caused by mutations in genes involved in the biosynthesis of coenzyme Q10 (CoQ10 ), a lipid component of the mitochondrial electron transport chain and an important antioxidant. However, the cellular mechanisms through which these mutations induce podocyte injury remain obscure. Here, we exploited the striking similarities between Drosophila nephrocytes and human podocytes to develop a Drosophila model of these renal diseases, and performed a systematic in vivo analysis assessing the role of CoQ10 pathway genes in renal function...
September 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28373296/a-proteomic-approach-to-identify-alterations-in-the-small-ubiquitin-like-modifier-sumo-network-during-controlled-mechanical-ventilation-in-rat-diaphragm-muscle
#19
Arvind Venkat Namuduri, Gabriel Heras, Jia Mi, Nicola Cacciani, Katarina Hörnaeus, Anne Konzer, Sara Bergström Lind, Lars Larsson, Stefano Gastaldello
The small ubiquitin-like modifier (SUMO) is as a regulator of many cellular functions by reversible conjugation to a broad number of substrates. Under endogenous or exogenous perturbations, the SUMO network becomes a fine sensor of stress conditions by alterations in the expression level of SUMO enzymes and consequently changing the status of SUMOylated proteins. The diaphragm is the major inspiratory muscle, which is continuously active under physiological conditions, but its structure and function is severely affected when passively displaced for long extents during mechanical ventilation (MV)...
June 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28326639/mesenchymal-stem-cells-attenuate-adriamycin-induced-nephropathy-by-diminishing-oxidative-stress-and-inflammation-via-downregulation-of-the-nf-kb
#20
In-Hwan Song, Kyong-Jin Jung, Tae-Jin Lee, Joo-Young Kim, Eon-Gi Sung, Young Chul Bae, Young Hoon Park
AIM: This study aimed to evaluate the molecular mechanism mitigating progress of chronic nephropathy by mesenchymal stem cells. METHODS: Rats were divided into normal control (Normal), ADR + vehicle (CON), and ADR + MSC (MSC) groups. Nephropathy was induced by ADR (4 mg/kg) and MSCs (2 × 10(6) ) were injected. Rats were sacrificed 1 or 6 weeks after ADR injection. NF-kB, MAPKs, inflammation, oxidative stress, profibrotic molecules, and nephrin expression were evaluated...
March 21, 2017: Nephrology
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