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Plant Autophagy

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https://www.readbyqxmd.com/read/29449851/divergent-n-deficiency-dependent-senescence-and-transcriptome-response-in-developmentally-old-and-young-brassica-napus-leaves
#1
Vajiheh Safavi-Rizi, Jürgen Franzaring, Andreas Fangmeier, Reinhard Kunze
In the spring oilseed rape (OSR) cultivar 'Mozart' grown under optimal N supply (N O ) or mild N deficiency (N L ) the transcriptome changes associated with progressing age until early senescence in developmentally old lower canopy leaves (leaf #4) and younger higher canopy leaves (leaf #8) were investigated. Twelve weeks old N O and N L plants appeared phenotypically and transcriptomically identical, but thereafter distinct nutrition-dependent differences in gene expression patterns in lower and upper canopy leaves emerged...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29448055/proteomic-characterization-of-withaferin-a-targeted-protein-networks-for-the-treatment-of-monoclonal-myeloma-gammopathies
#2
Martin Dom, Fritz Offner, Wim Vanden Berghe, Xaveer Van Ostade
Withaferin A (WA), a natural steroid lactone from the plant Withania somnifera, is often studied because of its antitumor properties. Although many in vitro and in vivo studies have been performed, the identification of Withaferin A protein targets and its mechanism of antitumor action remain incomplete. We used quantitative chemoproteomics and differential protein expression analysis to characterize the WA antitumor effects on a multiple myeloma cell model. Identified relevant targets were further validated by Ingenuity Pathway Analysis and Western blot and indicate that WA targets protein networks that are specific for monoclonal gammopathy of undetermined significance (MGUS) and other closely related disorders, such as multiple myeloma (MM) and Waldenström macroglobulinemia (WM)...
February 12, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29435647/the-unique-fold-and-lability-of-the-2fe-2s-clusters-of-neet-proteins-mediate-their-key-functions-in-health-and-disease
#3
REVIEW
Ola Karmi, Henri-Baptiste Marjault, Luca Pesce, Paolo Carloni, Jose' N Onuchic, Patricia A Jennings, Ron Mittler, Rachel Nechushtai
NEET proteins comprise a new class of [2Fe-2S] cluster proteins. In human, three genes encode for NEET proteins: cisd1 encodes mitoNEET (mNT), cisd2 encodes the Nutrient-deprivation autophagy factor-1 (NAF-1) and cisd3 encodes MiNT (Miner2). These recently discovered proteins play key roles in many processes related to normal metabolism and disease. Indeed, NEET proteins are involved in iron, Fe-S, and reactive oxygen homeostasis in cells and play an important role in regulating apoptosis and autophagy. mNT and NAF-1 are homodimeric and reside on the outer mitochondrial membrane...
February 12, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29422960/mecambridine-induces-potent-cytotoxic-effects-autophagic-cell-death-and-modulation-of-the-mtor-pi3k-akt-signaling-pathway-in-hsc-3-oral-squamous-cell-carcinoma-cells
#4
Na Lin, Zhiping Li, Deli Wang, Kewen Zheng, Yiyan Wu, Huiqi Wang
Plant secondary metabolites including alkaloids, demonstrate a complex diversity in their molecular scaffolds and exhibit tremendous pharmacological potential as anti-cancerous therapeutics. The present study aimed to evaluate the anticancer activity of a natural alkaloid, mecambridine, against human oral squamous cell carcinoma (OSCC). An MTT assay was used to evaluate cytotoxic effects of mecambridine on HSC-3 oral squamous cell carcinoma cells. Effects of mecambridine on autophagy-associated proteins were analyzed by western blotting...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29417220/ubiquitin-like-activating-enzymes-bcatg3-and-bcatg7-participate-in-development-and-pathogenesis-of-botrytis-cinerea
#5
Weichao Ren, Chengwei Sang, Dongya Shi, Xiushi Song, Mingguo Zhou, Changjun Chen
In eukaryotes, the ubiquitin-like (UBL) protein-activating enzymes play a crucial role in autophagy process, however, it is poorly characterized in filamentous fungi. Here, we investigated the functions of two UBL activating enzymes, BcAtg3 (E2) and BcAtg7 (E1) in the plant pathogenic fungus Botrytis cinerea. The physical interaction of BcAtg3 with BcAtg7 was demonstrated by yeast two-hybrid system. Subcellular localization assays showed that BcAtg3 diffused in cytoplasm, and BcAtg7 localized in cytoplasm as pre-autophagosomal structures (PAS)...
February 7, 2018: Current Genetics
https://www.readbyqxmd.com/read/29416826/role-of-ginsenosides-in-reactive-oxygen-species-mediated-anticancer-therapy
#6
REVIEW
Islam M D Sodrul, Chenying Wang, Xiangfeng Chen, Jing Du, Hongxiang Sun
Cancer is still a global public health problem, which is the leading cause of death in most countries. Ginseng has been used for centuries all over the world as a panacea that promotes longevity. As the king of herb plants, ginseng holds great promise as a new treatment option which is used either by itself or in combination with other medicinal ingredients that is widely accepted as complementary and alternative medicine in cancer therapy. Ginsenosides, the major pharmacologically active ingredients of ginseng, have been shown to have multiple medicinal effects including prominent anticancer activity...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29405978/new-insights-into-beclin-1-evolution-and-pan-malignancy-inhibitor-activity
#7
Stephen L Wechman, Anjan K Pradhan, Rob DeSalle, Swadesh K Das, Luni Emdad, Devanand Sarkar, Paul B Fisher
Autophagy is a functionally conserved self-degradation process that facilitates the survival of eukaryotic life via the management of cellular bioenergetics and maintenance of the fidelity of genomic DNA. The first known autophagy inducer was Beclin-1. Beclin-1 is expressed in multicellular eukaryotes ranging throughout plants to animals, comprising a nonmonophyllic group, as shown in this report via aggressive BLAST searches. In humans, Beclin-1 is a haploinsuffient tumor suppressor as biallelic deletions have not been observed in patient tumors clinically...
2018: Advances in Cancer Research
https://www.readbyqxmd.com/read/29405784/carvacrol-nanoemulsion-evokes-cell-cycle-arrest-apoptosis-induction-and-autophagy-inhibition-in-doxorubicin-resistant-a549-cell-line
#8
Imran Khan, Ashutosh Bahuguna, Monika Bhardwaj, Tejinder Pal Khaket, Sun Chul Kang
Carvacrol is a monoterpenoid flavonoid found abundantly in thyme plants. Its physiochemical instability and partial solubility in water is the principal limitation for its industrial use. Hence, we made a carvacrol nanoemulsion (CANE) using ultrasonication method and characterized it by dynamic light scattering (DLS) technique which revealed a negative surface charge (-29.89 mV) with 99.1 nm average droplet size. CANE effectively induced apoptosis in doxorubicin-resistant A549 lung carcinoma cells (A549 DR ) evident by the elevated expression of apoptotic proteins such as Bax, Cytochrome C, and Cleaved caspase 3 and 9...
February 6, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29392674/study-of-autophagy-in-plant-senescence
#9
Xuefei Cui, Jing Zheng, Jinxin Zheng, Qingqiu Gong
As a major intracellular degradation pathway, autophagy contributes to nutrient recycling and is indispensable during plant senescence. Here we describe methods used for investigating the autophagic process during leaf senescence. These include transcript analysis of core machinery autophagy genes, immunoblotting of ATG8, and microscopic observation of autophagosome formation.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29390157/vacuolar-protein-degradation-via-autophagy-provides-substrates-to-amino-acid-catabolic-pathways-as-an-adaptive-response-to-sugar-starvation-in-arabidopsis-thaliana
#10
Takaaki Hirota, Masanori Izumi, Shinya Wada, Amane Makino, Hiroyuki Ishida
The vacuolar lytic degradation of proteins releases free amino acids (AAs) that plants can use instead of sugars for respiratory energy production. Autophagy is a major cellular process leading to the transport of proteins into the vacuole for degradation. Here, we examine the contribution of autophagy to the AA metabolism response to sugar starvation in mature leaves of Arabidopsis thaliana. During sugar starvation arising from the exposure of wild-type (WT) plants to darkness, autophagic transport of chloroplast stroma, which contains most of the proteins in a leaf, into the vacuolar lumen was induced within two days...
January 30, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29380898/cytoprotective-effect-of-flavonoid-induced-autophagy-on-bisphosphonate-mediated-cell-death-in-osteoblast
#11
Jung-Han Kim, Hae-Mi Kang, Su-Bin Yu, Jae-Min Song, Chul-Hoon Kim, Bok-Joo Kim, Bong-Soo Park, Sang-Hun Shin, In-Ryoung Kim
With rapid economic growth and further developments in medical science, the entry into the aging population is currently increasing, as is the number of patients with metabolic diseases, such as hypertension, hyperlipidemia, heart disease, and diabetes. The current treatments for metabolic bone diseases, which are also on the rise, cause negative side effects. Bisphosphonates, which are used to treat osteoporosis, inhibit the bone resorption ability of osteoclasts and, during prolonged administration, cause bisphosphonate-related osteonecrosis of the jaw (BRONJ)...
January 30, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29378007/autophagy-controls-resource-allocations-and-protein-storage-accumulation-in-arabidopsis-seeds
#12
Julien Di Berardino, Anne Marmagne, Adeline Berger, Kohki Yoshimoto, Gwendal Cueff, Fabien Chardon, Céline Masclaux-Daubresse, Michèle Reisdorf-Cren
Autophagy is essential for nutrient recycling and plays a fundamental role in seed production and grain filling in plants. Autophagy participates in nitrogen remobilization at the whole plant level, and the seeds of autophagy mutants present abnormal C and N contents relative to wild type (WT). It is well known that autophagy genes are induced in leaves during senescence. The increased expression of autophagy genes in seeds has never been reported so far. We show here that most of the ATG genes are induced during seed maturation in Arabidopsis siliques and that ATG8f is the most highly expressed among them...
January 25, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29377765/autophagy-contributes-to-sulfonylurea-herbicide-tolerance-via-gcn2-independent-regulation-of-amino-acid-homeostasis
#13
Lun Zhao, Li Deng, Qing Zhang, Xue Jing, Meng Ma, Bin Yi, Jing Wen, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen
Sulfonylurea (SU) herbicides inhibit branched-chain amino acid (BCAA) biosynthesis by targeting acetolactate synthase. Plants have evolved target-site resistance and metabolic tolerance to SU herbicides; the GCN2 (general control non-repressible 2) pathway is also involved in SU tolerance. Here, we report a novel SU tolerance mechanism, autophagy, which we call 'homeostatic tolerance,' is involved in amino acid signaling in Arabidopsis. The activation and reversion of autophagy and GCN2 by the SU herbicide tribenuron-methyl (TM) and exogenous BCAA, respectively, confirmed that TM-induced BCAA starvation is responsible for the activation of autophagy and GCN2...
January 29, 2018: Autophagy
https://www.readbyqxmd.com/read/29369001/itraq-based-proteomics-analysis-of-autophagy-mediated-immune-responses-against-the-vascular-fungal-pathogen-verticillium-dahliae-in-arabidopsis
#14
Fu-Xin Wang, Yuan-Ming Luo, Zi-Qin Ye, Xue Cao, Jing-Nan Liang, Qian Wang, Yao Wu, Jia-He Wu, Hai-Yun Wang, Min Zhang, Huan-Qing Cheng, Gui-Xian Xia
The mechanisms underlying the functional link between autophagy and plant innate immunity remain largely unknown. In this study, we investigated the autophagy-mediated plant defense responses against Verticillium dahliae (V. dahliae) infection by comparative proteomics and cellular analyses. An assessment of the autophagy activity and disease development showed that autophagic processes were tightly related to the tolerance of Arabidopsis plant to Verticillium wilt. An isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics analysis was performed, and we identified a total of 780 differentially accumulated proteins (DAPs) between wild-type and mutant atg10-1 Arabidopsis plants upon V...
January 25, 2018: Autophagy
https://www.readbyqxmd.com/read/29365132/transcriptional-stimulation-of-rate-limiting-components-of-the-autophagic-pathway-improves-plant-fitness
#15
Elena A Minina, Panagiotis N Moschou, Ramesh R Vetukuri, Victoria Sanchez-Vera, Catarina Cardoso, Qinsong Liu, Pernilla H Elander, Kerstin Dalman, Mirela Beganovic, Jenny Lindberg Yilmaz, Sofia Marmon, Lana Shabala, Maria F Suarez, Karin Ljung, Ondrej Novák, Sergey Shabala, Sten Stymne, Daniel Hofius, Peter V Bozhkov
Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic compartment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5 and Atg8 with lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these systems causes autophagy-deficient phenotypes with reduced fitness and longevity. We show that Atg5 and E1-like enzyme, Atg7, are rate-limiting components of the Atg8-PE conjugation in Arabidopsis. Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation and autophagic flux...
January 20, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29362098/protein-degradation-mechanisms-modulate-abscisic-acid-signaling-and-responses-during-abiotic-stress
#16
Pawel Jurkiewicz, Henri Batoko
Abiotic stresses such as salinity, drought, high temperature or freezing can be perceived, in part, as a transient or permanent hyperosmotic stress by the plant cell. As sessile organisms, the detrimental effects of these environmental insults limit plants productivity but also their geographical distribution. Sensing and signaling events that detect the hyperosmotic (or simply osmotic) stress involve the cellular increase of active abscisic acid (ABA). The stress phytohormone ABA regulates fundamental growth and developmental processes in the plant by marshalling metabolic and gene-expression reprogramming...
February 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29350244/autophagy-related-atg-11-atg9-and-the-phosphatidylinositol-3-kinase-control-atg2-mediated-formation-of-autophagosomes-in-arabidopsis
#17
Sangwoo Kang, Kwang Deok Shin, Jeong Hun Kim, Taijoon Chung
Using quantitative assays for autophagy, we analyzed 4 classes of atg mutants, discovered new atg2 phenotypes and ATG gene interactions, and proposed a model of autophagosome formation in plants. Plant and other eukaryotic cells use autophagy to target cytoplasmic constituents for degradation in the vacuole. Autophagy is regulated and executed by a conserved set of proteins called autophagy-related (ATG). In Arabidopsis, several groups of ATG proteins have been characterized using genetic approaches. However, the genetic interactions between ATG genes have not been established and the relationship between different ATG groups in plants remains unclear...
January 19, 2018: Plant Cell Reports
https://www.readbyqxmd.com/read/29341143/loss-of-alkaline-ceramidase-inhibits-autophagy-in-arabidopsis-and-plays-an-important-role-during-environmental-stress-response
#18
Ping Zheng, Jian-Xin Wu, Sunil Kumar Sahu, Hong-Yun Zeng, Li-Qun Huang, Zhe Liu, Shi Xiao, Nan Yao
Sphingolipids, a class of bioactive lipids found in cell membranes, can modulate the biophysical properties of the membranes and play a critical role in signal transduction. Sphingolipids are involved in autophagy in humans and yeast, but their role in autophagy in plants is not well understood. In this study, we reported that the AtACER, an alkaline ceramidase that hydrolyzes ceramide to LCB, functions in autophagy process in Arabidopsis. Our empirical data showed that the loss of AtACER inhibited autophagy, while its overexpression promoted autophagy under nutrient, salinity, and oxidative stresses...
January 17, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29334999/the-plant-alkaloid-tetrandrine-inhibits-metastasis-via-autophagy-dependent-wnt-%C3%AE-catenin-and-metastatic-tumor-antigen-1-signaling-in-human-liver-cancer-cells
#19
Zhenxing Zhang, Ting Liu, Man Yu, Kangdi Li, Wenhua Li
BACKGROUND: Tetrandrine is a bisbenzylisoquinoline alkaloid isolated from the Chinese medicinal herb Stephania tetrandra S. Moore. We previously demonstrated that tetrandrine exhibits potent antitumor effects in many types of cancer cells. In this study, we investigated the effects of tetrandrine on human hepatocellular carcinoma (HCC) metastasis. METHODS: The invasion and migration effects were evaluated via wound healing and transwell assays. Immunofluorescence and western blotting analyses were used to investigate the levels of epithelial-mesenchymal transition (EMT)-related protein...
January 15, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29332356/%C3%AE-aescin-shows-potent-antiproliferative-activity-in-osteosarcoma-cells-by-inducing-autophagy-ros-generation-and-mitochondrial-membrane-potential-loss
#20
Zhao-Rui Liu, Li-Zhi Sun, Tang-Hong Jia, Dao-Fu Jia
PURPOSE: Osteosarcoma is one of the frequent bone tumor affecting mainly children and is associated with considerable mortality. The limited availability of anticancer drugs and less efficacious treatment options have led to poor survival rates of patients with osteosarcoma. Therefore, there is need to look for more viable treatment options and against this backdrop, natural products may prove handy. Therefore the aim of the present study was to evaluate the anticancer activity of a natural product of plant origin, β-aescin, against U2OS human osteosarcoma cells...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
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