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Vesicular stomatitis virus

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https://www.readbyqxmd.com/read/28335205/photosensitizer-embedded-polyacrylonitrile-nanofibers-as-antimicrobial-non-woven-textile
#1
Sarah L Stanley, Frank Scholle, Jiadeng Zhu, Yao Lu, Xiangwu Zhang, Xingci Situ, Reza A Ghiladi
Toward the objective of developing platform technologies for anti-infective materials based upon photodynamic inactivation, we employed electrospinning to prepare a non-woven textile comprised of polyacrylonitrile nanofibers embedded with a porphyrin-based cationic photosensitizer; termed PAN-Por((+)). Photosensitizer loading was determined to be 34.8 nmol/mg material; with thermostability to 300 °C. Antibacterial efficacy was evaluated against four bacteria belonging to the ESKAPE family of pathogens (Staphylococcus aureus; vancomycin-resistant Enterococcus faecium; Acinetobacter baumannii; and Klebsiella pneumonia), as well as Escherichia coli...
April 20, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28297751/preparation-of-vsv-g-viral-envelope-glycoprotein-from-pichia-pastoris-enhances-transfection-of-dna-into-animal-cells
#2
Xin Liu, Ying Dong, Jingquan Wang, Long Li, Zhenmin Zhong, Yun-Pan Li, Shao-Jun Chen, Yu-Cai Fu, Wen-Can Xu, Chi-Ju Wei
Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral and artificial viral vectors. The objective of this study was to establish a potential approach for large scale production of VSV-G. To this end, VSV-G was cloned with a N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut(s)) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, while the proliferation was reduced significantly under induction conditions...
March 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28289159/cutting-edge-innate-immune-augmenting-vesicular-stomatitis-virus-expressing-zika-virus-proteins-confers-protective-immunity
#3
Dillon Betancourt, Nina M G P de Queiroz, Tianli Xia, Jeonghyun Ahn, Glen N Barber
Zika virus (ZIKV) has become a serious public health concern because of its link to brain damage in developing human fetuses. Recombinant vesicular stomatitis virus (rVSV) was shown to be a highly effective and safe vector for the delivery of foreign immunogens for vaccine purposes. In this study, we generated rVSVs (wild-type and attenuated VSV with mutated matrix protein [VSVm] versions) that express either the full length ZIKV envelope protein (ZENV) alone or include the ZENV precursor to the membrane protein upstream of the envelope protein, and our rVSV-ZIKV constructs showed efficient immunogenicity in murine models...
March 13, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28274313/-establishment-of-esophageal-squamous-carcinoma-cell-lines-stably-over-expressing-llgl2
#4
Qiong Hu, Fu'an Xie, Xiaoqian Hong, Kuancan Liu
Objective To prepare a lentiviral vector expressing LLGL2 and establish KYSE450 and TE-1 cell lines for the stable expression of LLGL2. Methods The full-length LLGL2 sequence was amplified by high-fidelity PCR, and then it was inserted into pCDH-CMV-IRES-GFP-EF1-Puro vectors. The recombinant plasmid was confirmed by double enzyme digestion and sequencing. After co-infection of pCDH-CMV-LLGL2-IRES- GFP-EF1-Puro with vesicular stomatitis virus glycoprotein (VSVG) and PHR into HEK293T cells, the lentivirus was harvested and used for infecting esophageal squamous cell carcinoma cell lines including KYSE450 and TE-1 cells...
March 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28262484/the-antiviral-rna-interference-response-provides-resistance-to-lethal-arbovirus-infection-and-vertical-transmission-in-caenorhabditis-elegans
#5
Don B Gammon, Takao Ishidate, Lichao Li, Weifeng Gu, Neal Silverman, Craig C Mello
The recent discovery of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nematode Caenorhabditis elegans has stimulated interest in exploring virus-nematode interactions. However, OV infection is restricted to a small number of intestinal cells, even in nematodes defective in their antiviral RNA interference (RNAi) response, and is neither lethal nor vertically transmitted. Using a fluorescent reporter strain of the negative-sense ssRNA vesicular stomatitis virus (VSV), we show that microinjection of VSV particles leads to a dose-dependent, muscle tissue-tropic, lethal infection in C...
March 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28246125/zmpste24-defends-against-influenza-and-other-pathogenic-viruses
#6
Bishi Fu, Lingyan Wang, Shitao Li, Martin E Dorf
Zinc metallopeptidase STE24 (ZMPSTE24) is a transmembrane metalloprotease whose catalytic activity is critical for processing lamin A on the inner nuclear membrane and clearing clogged translocons on the endoplasmic reticulum. We now report ZMPSTE24 is a virus-specific effector that restricts enveloped RNA and DNA viruses, including influenza A, Zika, Ebola, Sindbis, vesicular stomatitis, cowpox, and vaccinia, but not murine leukemia or adenovirus. ZMPSTE24-mediated antiviral action is independent of protease activity...
February 28, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28237836/inhibitory-receptors-induced-by-vsv-viroimmunotherapy-are-not-necessarily-targets-for-improving-treatment-efficacy
#7
Kevin G Shim, Shane Zaidi, Jill Thompson, Tim Kottke, Laura Evgin, Karishma R Rajani, Matthew Schuelke, Christopher B Driscoll, Amanda Huff, Jose S Pulido, Richard G Vile
Systemic viroimmunotherapy activates endogenous innate and adaptive immune responses against both viral and tumor antigens. We have shown that therapy with vesicular stomatitis virus (VSV) engineered to express a tumor-associated antigen activates antigen-specific adoptively transferred T cells (adoptive cell therapy, ACT) in vivo to generate effective therapy. The overall goal of this study was to phenotypically characterize the immune response to VSV+ACT therapy and use the information gained to rationally improve combination therapy...
February 22, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28224119/vesicular-stomatitis-virus-vectored-multi-antigen-tuberculosis-vaccine-limits-bacterial-proliferation-in-mice-following-a-single-intranasal-dose
#8
Ming Zhang, Chunsheng Dong, Sidong Xiong
Tuberculosis (TB) remains a serious health problem worldwide, and an urgent need exists to improve or replace the available vaccine, Mycobacterium bovis bacillus Calmette-Guérin (BCG). Most vaccination protocols adapt two or three doses to induce long-term lasting immunity. Our previous study showed that the naked DNA encoding the triple-antigen fusion TFP846 (Rv3615c-Mtb10.4-Rv2660c) induced robust T cellular immune responses accompanying four inoculations against mycobacteria infection. However, a number of compliance issues exist in some areas lacking the appropriate medical infrastructure with multiple administrations...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28215695/inactivation-of-viruses-by-pasteurization-at-60%C3%A2-%C3%A2-c-for-10%C3%A2-h-with-and-without-40-glucose-as-stabilizer-during-a-new-manufacturing-process-of-%C3%AE-2-macroglobulin-from-cohn-fraction-iv
#9
Chaoji Huangfu, Yuyuan Ma, Junting Jia, Maomin Lv, Fengxuan Zhu, Xiaowei Ma, Xiong Zhao, Jingang Zhang
Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28213733/the-localization-of-a-heterologous-displayed-antigen-in-the-baculovirus-budded-virion-determines-the-type-and-strength-of-induced-adaptive-immune-response
#10
Eugenia Tavarone, Guido Nicolás Molina, Sabrina Amalfi, Andrea Peralta, Paula Molinari, Oscar Taboga
In the search of strategies of presentation of heterologous antigens to elicit humoral or cellular immune responses that modulate and properly potentiate each type of response, researchers have been studying baculovirus (BV) as vaccine vectors with promising results. For some years, several research groups explored different antigen presentation approaches using the BV AcNPV by expressing polypeptides on the surface of budded virions or by de novo synthesis of heterologous antigens by transduction of mammalian cells...
February 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28188244/structural-intermediates-in-the-fusion-associated-transition-of-vesiculovirus-glycoprotein
#11
Eduard Baquero, Aurélie A Albertini, Hélène Raux, Abbas Abou-Hamdan, Elisabetta Boeri-Erba, Malika Ouldali, Linda Buonocore, John K Rose, Jean Lepault, Stéphane Bressanelli, Yves Gaudin
Vesiculoviruses enter cells by membrane fusion, driven by a large, low-pH-induced, conformational change in the fusion glycoprotein G that involves transition from a trimeric pre-fusion toward a trimeric post-fusion state via monomeric intermediates. Here, we present the structure of the G fusion protein at intermediate pH for two vesiculoviruses, vesicular stomatitis virus (VSV) and Chandipura virus (CHAV), which is responsible for deadly encephalopathies. First, a CHAV G crystal structure shows two intermediate conformations forming a flat dimer of heterodimers...
March 1, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28166243/development-of-a-gexp-multiplex-pcr-assay-for-the-simultaneous-detection-and-differentiation-of-six-cattle-viruses
#12
Qing Fan, Zhixun Xie, Zhiqin Xie, Xianwen Deng, Liji Xie, Li Huang, Sisi Luo, Jiaoling Huang, Yanfang Zhang, Tingting Zeng, Sheng Wang, Jiabo Liu, Yaoshan Pang
Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss every year in many parts of the world. A rapid and high-throughput GenomeLab Gene Expression Profiler (GeXP) analyzer-based multiplex PCR assay was developed for the simultaneous detection and differentiation of these six cattle viruses. Six pairs of chimeric primers consisting of both the gene-specific primer and a universal primer were designed and used for amplification...
2017: PloS One
https://www.readbyqxmd.com/read/28152326/safety-and-immunogenicity-of-gamevac-combi-a-heterologous-vsv-and-ad5-vectored-ebola-vaccine-an-open-phase-i-ii-trial-in-healthy-adults-in-russia
#13
I V Dolzhikova, O V Zubkova, A I Tukhvatulin, A S Dzharullaeva, N M Tukhvatulina, D V Shcheblyakov, M M Shmarov, E A Tokarskaya, Y V Simakova, D A Egorova, D N Scherbinin, I L Tutykhina, A A Lysenko, A V Kostarnoy, P G Gancheva, T A Ozharovskaya, B V Belugin, L V Kolobukhina, V B Pantyukhov, S I Syromyatnikova, I V Shatokhina, T V Sizikova, I G Rumyantseva, A F Andrus, N V Boyarskaya, A N Voytyuk, V F Babira, S V Volchikhina, D A Kutaev, A N Bel'skih, K V Zhdanov, S M Zakharenko, S V Borisevich, D Y Logunov, B S Naroditsky, A L Gintsburg
Ebola hemorrhagic fever, also known as Ebola virus disease or EVD, is one of the most dangerous viral diseases in humans and animals. In this open-label, dose-escalation clinical trial, we assessed the safety, side effects, and immunogenicity of a novel, heterologous prime-boost vaccine against Ebola, which was administered in 2 doses to 84 healthy adults of both sexes between 18 and 55 years. The vaccine consists of live-attenuated recombinant vesicular stomatitis virus (VSV) and adenovirus serotype-5 (Ad5) expressing Ebola envelope glycoprotein...
February 2, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28148802/recombinant-isfahan-virus-and-vesicular-stomatitis-virus-vaccine-vectors-provide-durable-multivalent-single-dose-protection-against-lethal-alphavirus-challenge
#14
Farooq Nasar, Demetrius Matassov, Robert L Seymour, Theresa Latham, Rodion V Gorchakov, Rebecca M Nowak, Grace Leal, Stefan Hamm, John H Eldridge, Robert B Tesh, David K Clarke, Scott C Weaver
The demonstrated clinical efficacy of a recombinant vesicular stomatitis virus (rVSV) vaccine vector has stimulated the investigation of additional serologically distinct Vesiculovirus vectors as therapeutic and/or prophylactic vaccine vectors to combat emerging viral diseases. Amongst these viral threats are encephalitic alphaviruses, Venezuelan and eastern equine encephalitic viruses (VEEV, EEEV), which have demonstrated potential for natural disease outbreaks, yet no licensed vaccines are available in the event of an epidemic...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28137780/arabidopsis-hap2-gcs1-is-a-gamete-fusion-protein-homologous-to-somatic-and-viral-fusogens
#15
Clari Valansi, David Moi, Evgenia Leikina, Elena Matveev, Martín Graña, Leonid V Chernomordik, Héctor Romero, Pablo S Aguilar, Benjamin Podbilewicz
Cell-cell fusion is inherent to sexual reproduction. Loss of HAPLESS 2/GENERATIVE CELL SPECIFIC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion...
March 6, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28088779/comprehensive-proteome-analysis-of-lysosomes-reveals-the-diverse-function-of-macrophages-in-immune-responses
#16
Yanpan Gao, Yanyu Chen, Shaohua Zhan, Wenhao Zhang, Feng Xiong, Wei Ge
Phagocytosis and autophagy in macrophages have been shown to be essential to both innate and adaptive immunity. Lysosomes are the main catabolic subcellular organelles responsible for degradation and recycling of both extracellular and intracellular material, which are the final steps in phagocytosis and autophagy. However, the molecular mechanisms underlying lysosomal functions after infection remain obscure. In this study, we conducted a quantitative proteomics analysis of the changes in constitution and glycosylation of proteins in lysosomes derived from murine RAW 264...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28077641/chikungunya-influenza-nipah-and-semliki-forest-chimeric-viruses-with-vesicular-stomatitis-virus-actions-in-the-brain
#17
Anthony N van den Pol, Guochao Mao, Anasuya Chattopadhyay, John K Rose, John N Davis
Recombinant vesicular stomatitis virus (VSV)-based chimeric viruses that include genes from other viruses show promise as vaccines and oncolytic viruses. However, the critical safety concern is the neurotropic nature conveyed by the VSV glycoprotein. VSVs that include the VSV glycoprotein (G) gene, even in most recombinant attenuated strains, can still show substantial adverse or lethal actions in the brain. Here, we test 4 chimeric viruses in the brain, including those in which glycoprotein genes from Nipah, chikungunya (CHIKV), and influenza H5N1 viruses were substituted for the VSV glycoprotein gene...
March 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28077601/a-new-rna-based-adjuvant-enhances-virus-specific-vaccine-responses-by-locally-triggering-tlr-and-rlh-dependent-effects
#18
Annett Ziegler, Claudia Soldner, Stefan Lienenklaus, Julia Spanier, Stephanie Trittel, Peggy Riese, Thomas Kramps, Siegfried Weiss, Regina Heidenreich, Edith Jasny, Carlos A Guzmán, Karl-Josef Kallen, Mariola Fotin-Mleczek, Ulrich Kalinke
Among innovative adjuvants conferring a Th1-shift, RNAdjuvant is a promising candidate. This adjuvant consists of a 547-nt uncapped noncoding ssRNA containing polyU repeats that is stabilized by a cationic carrier peptide. Whereas vaccination of mice with an influenza subunit vaccine induced moderate virus-specific IgG1, vaccination together with RNAdjuvant significantly enhanced this IgG1 and additionally promoted the formation of IgG2b/c, which is indicative of Th1 responses. Furthermore, such sera neutralized influenza virus, whereas this effect was not detected upon vaccination with the subunit vaccine alone...
January 11, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28053102/5-phospho-rna-acceptor-specificity-of-gdp-polyribonucleotidyltransferase-of-vesicular-stomatitis-virus-in-mrna-capping
#19
Minako Ogino, Tomoaki Ogino
The GDP polyribonucleotidyltransferase (PRNTase) domain of the multifunctional L protein of rhabdoviruses, such as vesicular stomatitis virus (VSV) and rabies virus, catalyzes the transfer of 5'-phospho-RNA (pRNA) from 5'-triphospho-RNA (pppRNA) to GDP via a covalent enzyme-pRNA intermediate to generate a 5'-cap structure (GpppA). Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that the Michaelis constants for GDP and pppAACAG (VSV mRNA-start sequence) are 0.03 and 0.4 μM, respectively...
March 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28049150/fosl1-inhibits-type-i-interferon-responses-to-malaria-and-viral-infections-by-blocking-tbk1-and-traf3-trif-interactions
#20
Baowei Cai, Jian Wu, Xiao Yu, Xin-Zhuan Su, Rong-Fu Wang
Innate immune response plays a critical role in controlling invading pathogens, but such an immune response must be tightly regulated. Insufficient or overactivated immune responses may lead to harmful or even fatal consequences. To dissect the complex host-parasite interactions and the molecular mechanisms underlying innate immune responses to infections, here we investigate the role of FOS-like antigen 1 (FOSL1) in regulating the host type I interferon (IFN-I) response to malaria parasite and viral infections...
January 3, 2017: MBio
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