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Vesicular stomatitis virus

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https://www.readbyqxmd.com/read/29306950/immune-consequences-of-in-vitro-infection-of-human-peripheral-blood-leukocytes-with-vesicular-stomatitis-virus
#1
Tomasz Tomczyk, Grażyna Wróbel, Radosław Chaber, Iwona Siemieniec, Egbert Piasecki, Małgorzata Krzystek-Korpacka, Beata U Orzechowska
BACKGROUND: Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. Our study aimed to assess the overall immunological consequences of ex vivo infection of PBLs with VSV. METHODS: Phenotypic analysis of lymphocyte subsets and apoptosis were evaluated with flow cytometry. Caspase 3/7 activity was detected by luminescence assay...
January 6, 2018: Journal of Innate Immunity
https://www.readbyqxmd.com/read/29304309/susceptibility-of-zebrafish-to-vesicular-stomatitis-virus-infection
#2
Jorge Guerra-Varela, Maite Baz-Martínez, Sabela Da Silva-Álvarez, Ana Paula Losada, María Isabel Quiroga, Manuel Collado, Carmen Rivas, Laura Sánchez
The zebrafish, Danio rerio, has become recognized as a valuable model for infectious diseases. Here we evaluated the susceptibility of zebrafish to be infected with the mammalian vesicular stomatitis virus (VSV). Both zebrafish cells and embryos were highly susceptible to VSV infection. Mortalities exceeded 80% in infected embryos and were preceded by the invasion of the central nervous system by VSV. Live imaging of the infection with GFP-VSV as well as virus titration from infected fish confirmed the viral replication...
January 5, 2018: Zebrafish
https://www.readbyqxmd.com/read/29302255/species-specific-antiviral-activity-of-porcine-interferon-%C3%AE-8-ifn%C3%AE-8
#3
Eunhye Kim, Hyunjhung Jhun, Joohee Kim, Unjoo Park, Seunghyun Jo, Areum Kwak, Sinae Kim, Tam T Nguyen, Yongsun Kang, Insoo Choi, Joongbok Lee, Heijun Kim, Younghyun Kim, Siyoung Lee, Soohyun Kim
Interferons (IFNs) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 IFN. The type 1 IFN consists of IFNα, IFNβ, IFNτ, and IFNω whereas the type 2 IFN consists of only IFNγ, which is a key cytokine driving T helper cell type 1 immunity. IFNλ belongs to the type 3 IFN, which is also known as IL-28 and IL-29 possessing antiviral activities. Type 1 IFN is produced by viral infection whereas type 2 IFN is induced by mitogenic or antigenic T-cell stimuli. The IFNτ of bovine was first discovered in an ungulate ruminant recognition hormone...
December 2017: Immune Network
https://www.readbyqxmd.com/read/29296856/measles-virus-envelope-pseudotyped-lentiviral-vectors-transduce-quiescent-human-hscs-at-an-efficiency-without-precedent
#4
Camille Lévy, Fouzia Amirache, Anais Girard-Gagnepain, Cecilia Frecha, Francisco J Roman-Rodríguez, Ornellie Bernadin, Caroline Costa, Didier Nègre, Alejandra Gutierrez-Guerrero, Lenard S Vranckx, Isabelle Clerc, Naomi Taylor, Lars Thielecke, Kerstin Cornils, Juan A Bueren, Paula Rio, Rik Gijsbers, François-Loïc Cosset, Els Verhoeyen
Hematopoietic stem cell (HSC)-based gene therapy trials are now moving toward the use of lentiviral vectors (LVs) with success. However, one challenge in the field remains: efficient transduction of HSCs without compromising their stem cell potential. Here we showed that measles virus glycoprotein-displaying LVs (hemagglutinin and fusion protein LVs [H/F-LVs]) were capable of transducing 100% of early-acting cytokine-stimulated human CD34+ (hCD34+) progenitor cells upon a single application. Strikingly, these H/F-LVs also allowed transduction of up to 70% of nonstimulated quiescent hCD34+ cells, whereas conventional vesicular stomatitis virus G (VSV-G)-LVs reached 5% at the most with H/F-LV entry occurring exclusively through the CD46 complement receptor...
October 24, 2017: Blood Advances
https://www.readbyqxmd.com/read/29288049/development-of-a-recombinase-polymerase-amplification-combined-with-lateral-flow-dipstick-assay-for-detection-of-bovine-ephemeral-fever-virus
#5
Peili Hou, Guimin Zhao, Hongmei Wang, Chengqiang He, Yanjun Huan, Hongbin He
Bovine ephemeral fever virus (BEFV), identified as the causative pathogen of bovine ephemeral fever (BEF), is responsible for increasing numbers of epidemics/outbreaks and has a significant harmful effect on the livestock industry. Therefore, a rapid detection assay is imperative for BEFV diagnosis. In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. RPA primers and LF probes were designed by targeting the specific G gene, and the amplification product can be visualized on a simple lateral flow dipstick with the naked eyes...
December 26, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29281729/histone-demethylase-lsd1-restricts-influenza-a-virus-infection-by-erasing-ifitm3-k88-monomethylation
#6
Jiaoyu Shan, Binbin Zhao, Zhao Shan, Jia Nie, Rong Deng, Rui Xiong, Andy Tsun, Weiqi Pan, Hanzhi Zhao, Ling Chen, Ying Jin, Zhikang Qian, Kawing Lui, Rui Liang, Dan Li, Bing Sun, Dimitri Lavillette, Ke Xu, Bin Li
The histone demethylase LSD1 has been known as a key transcriptional coactivator for DNA viruses such as herpes virus. Inhibition of LSD1 was found to block viral genome transcription and lytic replication of DNA viruses. However, RNA virus genomes do not rely on chromatin structure and histone association, and the role of demethylase activity of LSD1 in RNA virus infections is not anticipated. Here, we identify that, contrary to its role in enhancing DNA virus replication, LSD1 limits RNA virus replication by demethylating and activating IFITM3 which is a host restriction factor for many RNA viruses...
December 27, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29277269/chromosome-19-micrornas-exert-antiviral-activity-independent-from-type-iii-interferon-signaling
#7
Avraham Bayer, Nicholas J Lennemann, Yingshi Ouyang, Elena Sadovsky, Megan A Sheridan, R Michael Roberts, Carolyn B Coyne, Yoel Sadovsky
INTRODUCTION: Cultured primary human trophoblasts (PHT), derived from term placentas, are relatively resistant to infection by diverse viruses. The resistance can be conferred to non-trophoblastic cells by pre-exposing them to medium that was conditioned by PHT cells. This antiviral effect is mediated, at least in part, by microRNAs (miRNA) expressed from the chromosome 19 microRNA cluster (C19MC). Recently we showed that PHT cells and cells pre-exposed to PHT medium are also resistant to infection by Zika virus (ZIKV), an effect mediated by the constitutive release of the type III interferons (IFN) IFN lambda-1 and IFN lambda-2 in trophoblastic medium...
January 2018: Placenta
https://www.readbyqxmd.com/read/29276724/infection-of-caenorhabditis-elegans-with-vesicular-stomatitis-virus-via-microinjection
#8
Adam Martin, Emily A Rex, Takao Ishidate, Rueyling Lin, Don B Gammon
Over the past 15 years, the free-living nematode, Caenorhabditis elegans has become an important model system for exploring eukaryotic innate immunity to bacterial and fungal pathogens. More recently, infection models using either natural or non-natural nematode viruses have also been established in C. elegans. These models offer new opportunities to use the nematode to understand eukaryotic antiviral defense mechanisms. Here we report protocols for the infection of C. elegans with a non-natural viral pathogen, vesicular stomatitis virus (VSV) through microinjection...
November 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29246504/a-recombinant-vsv-vectored-mers-cov-vaccine-induces-neutralizing-antibody-and-t-cell-responses-in-rhesus-monkeys-after-single-dose-immunization
#9
Renqiang Liu, Jinliang Wang, Yu Shao, Xijun Wang, Huilei Zhang, Lei Shuai, Jinying Ge, Zhiyuan Wen, Zhigao Bu
Middle East respiratory syndrome coronavirus (MERS-CoV) has been a highly threatening zoonotic pathogen since its outbreak in 2012. Similar to SARS-CoV, MERS-CoV belongs to the coronavirus family and can induce severe respiratory symptoms in humans, with an average case fatality rate of 35% according to the World Health Organization. Spike (S) protein of MERS-CoV is immunogenic and can induce neutralizing antibodies, thus is a potential major target for vaccine development. Here we constructed a chimeric virus based on the vesicular stomatitis virus (VSV) in which the G gene was replaced by MERS-CoV S gene (VSVΔG-MERS)...
December 12, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29224776/development-of-a-sybr-green-i-real-time-pcr-for-detection-and-quantitation-of-orthopoxvirus-by-using-ectromelia-virus
#10
Wenyu Cheng, Xiaobing He, Huaijie Jia, Guohua Chen, Cong Wang, Jun Zhang, Zhizhong Jing
Ectromelia virus (ECTV) is the causative agent of mousepox, which has devastating effects in laboratory-mouse colonies and causes economic loss in biomedical research. More importantly, ECTV has been extensively used as an excellent model for studies of the pathogenesis and immunobiology of human smallpox. A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by using ECTV in this study. Primers targeted to the highly conserved region of major core protein P4b gene of orthopoxvirus were designed and the standard plasmid was constructed...
December 7, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29216247/the-glycoprotein-of-vesicular-stomatitis-virus-promotes-release-of-virus-like-particles-from-tetherin-positive-cells
#11
Constantin Brinkmann, Markus Hoffmann, Anastasia Lübke, Inga Nehlmeier, Annika Krämer-Kühl, Michael Winkler, Stefan Pöhlmann
Vesicular stomatitis virus (VSV) release from infected cells is inhibited by the interferon (IFN)-inducible antiviral host cell factor tetherin (BST-2, CD317). However, several viruses encode tetherin antagonists and it is at present unknown whether residual VSV spread in tetherin-positive cells is also promoted by a virus-encoded tetherin antagonist. Here, we show that the viral glycoprotein (VSV-G) antagonizes tetherin in transfected cells, although with reduced efficiency as compared to the HIV-1 Vpu protein...
2017: PloS One
https://www.readbyqxmd.com/read/29213292/ifn-%C3%AE-1-in-cho-cells-its-expression-and-biological-activity
#12
Wu-Mei Yuan, Wan-Ju Zhang, Fen-Lian Ma, Jin-Song Li, Qian Zhang, Li-Shu Zheng
Background: Many studies have investigated the characteristics and biological activities of type III interferon (IFN), finding that it has similar features to type I IFN but also unique actions because it is recognized by a different receptor. Results: A full-length recombinant human IFN-λ1 (rhIFN-λ1) cDNA was cloned into the pDF expression vector and stably expressed in Flp-In-CHO cells. After four purification steps (ammonium sulfate precipitation, SP Sepharose chromatography, Blue Sepharose 6 fast flow affinity chromatography and molecular sieve chromatography), the rhIFN-λ1 had a purity of about 90% and was found to have the predicted biological activities...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29212930/control-of-htlv-1-infection-by-eliminating-envelope-protein-positive-cells-with-recombinant-vesicular-stomatitis-viruses-encoding-htlv-1-primary-receptor
#13
Kenta Tezuka, Kazu Okuma, Madoka Kuramitsu, Sahoko Matsuoka, Reiko Tanaka, Yuetsu Tanaka, Isao Hamaguchi
Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia (ATL), which is frequently resistant to current available therapies and has a very poor prognosis. To prevent the development of ATL among carriers it is important to control HTLV-1-infected cells in infected individuals. Therefore, the establishment of novel therapies with drugs specifically targeting infected cells is urgently required. This study aimed to develop a potential therapy by generating recombinant vesicular stomatitis viruses (rVSVs) that lack an envelope glycoprotein G and instead encode HTLV-1 receptor(s) with human glucose transporter 1 (GLUT1), neuropilin 1 (NRP1), or heparan sulfate proteoglycans (HSPGs) including syndecan 1 (SDC1), designated as VSVΔG-GL, VSVΔG-NP, or VSVΔG-SD, respectively...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29209895/antiviral-activity-and-metal-ion-binding-properties-of-some-2-hydroxy-3-methoxyphenyl-acylhydrazones
#14
M Carcelli, E Fisicaro, C Compari, L Contardi, D Rogolino, C Solinas, A Stevaert, L Naesens
Here we report on the results obtained from an antiviral screening, including herpes simplex virus, vaccinia virus, vesicular stomatitis virus, Coxsackie B4 virus or respiratory syncytial virus, parainfluenza-3 virus, reovirus-1 and Punta Toro virus, of three 2-hydroxy-3-methoxyphenyl acylhydrazone compounds in three cell lines (i.e. human embryonic lung fibroblast cells, human cervix carcinoma cells, and African Green monkey kidney cells). Interesting antiviral EC50 values are obtained against herpes simplex virus-1 and vaccinia virus...
December 5, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29206076/assessment-of-the-ability-of-v920-recombinant-vesicular-stomatitis-zaire-ebolavirus-vaccine-to-replicate-in-relevant-arthropod-cell-cultures-and-vector-species
#15
Nicholas A Bergren, Megan R Miller, Thomas P Monath, Rebekah C Kading
V920, rVSVΔG-ZEBOV-GP, is a recombinant vesicular stomatitis-Zaire ebolavirus vaccine which has shown an acceptable safety profile and provides a protective immune response against Ebola virus disease (EVD) induced by Zaire ebolavirus in humans. The purpose of this study was to determine whether the V920 vaccine is capable of replicating in arthropod cell cultures of relevant vector species and of replicating in live mosquitoes. While the V920 vaccine replicated well in Vero cells, no replication was observed in Anopheles or Aedes mosquito, Culicoides biting midge, or Lutzomyia sand fly cells, nor in live Culex or Aedes mosquitoes following exposure through intrathoracic inoculation or feeding on a high-titer infectious blood meal...
December 5, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29189090/characterization-of-whooper-swan-cygnus-cygnus-interferon-%C3%AE-prokaryotic-expression-biological-activities-and-physicochemical-characteristics
#16
Hongjing Zhao, Yu Wang, Yizhi Shao, Juanjuan Liu, Jinglun Li, Hui Zong, Mingwei Xing
Whopper swan Cygnus cygnus interferon alpha (CyIFN-α) gene was amplified from genomic DNA in the liver of whooper swan, which encodes a 192-amino acid protein containing a 29-amino acid signal peptide. Bioinformatics analysis showed it containes 7 cysteine residues and 5 alpha helices. After expression in prokaryote, purification and renaturation, CyIFN-α was analyzed on its physicochemical property and antiviral activity. In chicken embryo fibroblasts, CyIFN-α exerted more superior anti-vesicular stomatitis virus (VSV), avian influenza virus (AIV), and Newcastle disease virus (NDV) activity than chicken IFN-α, an effective therapeutic agent used for viral infections clinically...
November 30, 2017: Journal of Interferon & Cytokine Research
https://www.readbyqxmd.com/read/29180817/virus-replicon-particle-vaccines-expressing-nucleoprotein-of-influenza-a-virus-mediate-enhanced-inflammatory-responses-in-pigs
#17
Meret E Ricklin, Sylvie Python, Nathalie J Vielle, Daniel Brechbühl, Beatrice Zumkehr, Horst Posthaus, Gert Zimmer, Nicolas Ruggli, Artur Summerfield
Studies in the mouse model indicate that the nucleoprotein of influenza A virus represents an interesting vaccine antigen being well conserved across subtypes of influenza virus but still able to induce protective immune responses. Here we show that immunizations of pigs with vesicular stomatitis virus- and classical swine fever virus-derived replicon (VRP) particles expressing the nucleoprotein (NP) of H1N1 A/swine/Belzig/2/01 induced potent antibody and T-cell responses against influenza A virus. In contrast to a conventional whole inactivated virus vaccine, the VRP vaccines induced both NP-specific CD4 and CD8 T cells responses, including interferon-γ and tumor-necrosis-factor dual-secreting cell...
November 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29173589/eif3i-affects-vesicular-stomatitis-virus-growth-by-interacting-with-matrix-protein
#18
Wei Pan, Deguang Song, Wenqi He, Huijun Lu, Yungang Lan, Hongli Li, Feng Gao, Kui Zhao
The matrix protein of vesicular stomatitis virus (VSV) performs multiple functions during viral genome replication and virion production and is involved in modulating multiple host signaling pathways that favor virus replication. To perform numerous functions within infected cells, the M protein needs to recruit cellular partners. To better understand the role of M during VSV replication, we looked for interacting partners by using the two-hybrid system. The eukaryotic translation initiation factor 3, subunit i (eIF3i) was identified to be an M-binding partner, and this interaction was validated by GST pull-down and laser confocal assays...
December 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29160106/display-of-the-albumin-binding-domain-in-the-envelope-improves-lentiviral-vector-bioavailability
#19
Antonio Rodriguez, Guillermo Garaulet, Juan José Lazcano, Hernán Alarcón, Sergio de Frutos, Jorge Luis Martínez-Torrecuadrada
Vesicular stomatitis virus G glycoprotein (VSVg) is extensively used for retroviral and lentiviral vector (LV) pseudotyping. However, VSVg pseudotyped vectors are serum inactivated, blocking the in vivo gene delivery. Several strategies have been employed to prevent complement inactivation, including chemical and genetic envelope modifications. Here we have employed the streptococcal Albumin Binding Domain (ABD) to generate a construct to express ABD as a GPI-anchored protein. LV particles bearing ABD are able to bind bovine and human serum albumin in vitro...
November 21, 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/29158470/comparative-oncology-evaluation-of-intravenous-recombinant-oncolytic-vesicular-stomatitis-virus-therapy-in-spontaneous-canine-cancer
#20
Shruthi Naik, Gina D Galyon, Nathan J Jenks, Michael B Steele, Amber C Miller, Sara D Allstadt, Lukkana Suksanpaisan, Kah Whye Peng, Mark J Federspiel, Stephen J Russell, Amy K LeBlanc
Clinical translation of intravenous therapies to treat disseminated or metastatic cancer is imperative. Comparative oncology, the evaluation of novel cancer therapies in animals with spontaneous cancer, can be utilized to inform and accelerate clinical translation. Preclinical murine studies demonstrate that single shot systemic therapy with a VSV-IFNβ-NIS, a novel recombinant oncolytic Vesicular stomatitis virus (VSV), can induce curative remission in tumor bearing mice. Clinical translation of VSV-IFNβ-NIS therapy is dependent on comprehensive assessment of clinical toxicities, virus shedding, pharmacokinetics, and efficacy in clinically relevant models...
November 20, 2017: Molecular Cancer Therapeutics
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