keyword
MENU ▼
Read by QxMD icon Read
search

Toxoplasma gondii

keyword
https://www.readbyqxmd.com/read/29330674/advances-in-serological-imaging-techniques-and-molecular-diagnosis-of-toxoplasma-gondii-infection
#1
REVIEW
Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
BACKGROUND: Toxoplasmosis is worldwide distributed zoonotic infection disease with medical importance in immunocompromised patients, pregnant women and congenitally infected newborns. Having basic information on the traditional and new developed methods is essential for general physicians and infectious disease specialists for choosing a suitable diagnostic approach for rapid and accurate diagnosis of the disease and, consequently, timely and effective treatment. METHODS: We conducted English literature searches in PubMed from 1989 to 2016 using relevant keywords and summarized the recent advances in diagnosis of toxoplasmosis...
January 12, 2018: Infection
https://www.readbyqxmd.com/read/29329617/dna-detection-of-trypanosoma-evansi-diagnostic-validity-of-a-new-assay-based-on-loop-mediated-isothermal-amplification-lamp
#2
Qunbo Tong, Rui Chen, Qingming Kong, Julie Goossens, Magdalena Radwanska, Di Lou, Jianzu Ding, Bin Zheng, Yixiu Fu, Tianping Wang, Magez Stefan, Shaohong Lu
Trypanosoma evansi (T. evansi) is the most widely spread pathogenic trypanosome in the world. The control of trypanosomiasis depends on accurate diagnosis and effective treatment. Focusing on the presence of T. evansi in Asia, we developed a detection assay based on tracing phosphate ions (Pi) generated during LAMP targeting the variant surface glycoprotein (VSG) gene of Rode Trypanozoon antigenic type 1.2 (RoTat 1.2 VSG). The diagnostic potential as well as the use of the assay as a test-of-cure method after berenil treatment, was assessed in mice at different time points of infection...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29326391/zoonotic-bacteria-and-parasites-found-in-raw-meat-based-diets-for-cats-and-dogs
#3
Freek P J van Bree, Gertie C A M Bokken, Robin Mineur, Frits Franssen, Marieke Opsteegh, Joke W B van der Giessen, Len J A Lipman, Paul A M Overgaauw
Feeding raw meat-based diets (RMBDs) to companion animals has become increasingly popular. Since these diets may be contaminated with bacteria and parasites, they may pose a risk to both animal and human health. The purpose of this study was to test for the presence of zoonotic bacterial and parasitic pathogens in Dutch commercial RMBDs. We analysed 35 commercial frozen RMBDs from eight different brands. Escherichia coli serotype O157:H7 was isolated from eight products (23 per cent) and extended-spectrum beta-lactamases-producing E coli was found in 28 products (80 per cent)...
January 13, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29324806/immunocompetent-host-develops-mild-intestinal-inflammation-in-acute-infection-with-toxoplasma-gondii
#4
Paulo da Silva Watanabe, Aline Rosa Trevizan, Saulo Euclides Silva-Filho, Marcelo Biondaro Góis, João Luiz Garcia, Roberto Kenji Nakamura Cuman, Ana Cristina Breithaupt-Faloppa, Débora de Mello Gonçales Sant Ana, Gessilda de Alcantara Nogueira de Melo
Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis, common zoonosis among vertebrates and high incidence worldwide. During the infection, the parasite needs to transpose the intestinal barrier to spread throughout the body, which may be a trigger for an inflammatory reaction. This work evaluated the inflammatory alterations of early T. gondii infection in peripheral blood cells, in the mesenteric microcirculation, and small intestinal tissue by measurement of MPO (myeloperoxidase) activity and NO (nitric oxide) level in rats...
2018: PloS One
https://www.readbyqxmd.com/read/29323662/crispr-cas9-based-genome-wide-screening-of-toxoplasma-gondii
#5
Saima M Sidik, Diego Huet, Sebastian Lourido
Apicomplexan parasites, such as Toxoplasma gondii, cause extensive morbidity and mortality in humans and livestock, highlighting the need for a deeper understanding of their molecular biology. Although techniques for the generation of targeted gene disruptions have long been available for apicomplexans, such methods are not readily scalable to the entire genome. We recently used CRISPR-Cas9 to disrupt all nuclear protein-coding genes in T. gondii using a pooled format. The method relies on transfection of a guide RNA library into parasites constitutively expressing Cas9...
January 2018: Nature Protocols
https://www.readbyqxmd.com/read/29322275/outcome-of-polymerase-chain-reaction-pcr-analysis-in-100-suspected-cases-of-infectious-uveitis
#6
Ranju Kharel Sitaula, M K Janani, H N Madhavan, Jyotirmay Biswas
BACKGROUND: Polymerase chain reaction (PCR) analysis is an important tool in the diagnosis of infectious uveitis. A retrospective, interventional study of PCR analysis of ocular fluid in suspected infectious uveitis cases between January 2014 to July 2016 was done. Nested, real-time and broad range PCR was performed for detection of the genome of Mycobacterium tuberculosis, herpes virus family, Chikungunya virus, Toxoplasma gondii, fungus, eubacterium and propionibacterium acne. RESULTS: Total of 100 cases included, mean age was 39...
January 10, 2018: Journal of Ophthalmic Inflammation and Infection
https://www.readbyqxmd.com/read/29320816/il-12-and-il-23-production-in-toxoplasma-gondii-or-lps-treated-jurkat-t-cells-via-pi3k-and-mapk-signaling-pathways
#7
Hassan Ahmed Hassan Ahmed Ismail, Byung-Hun Kang, Jae-Su Kim, Jae-Hyung Lee, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
IL-12 and IL-23 are closely related in structure, and have been shown to play crucial roles in regulation of immune responses. However, little is known about the regulation of these cytokines in T cells. Here, we investigated the roles of PI3K and MAPK pathways in IL-12 and IL-23 production in human Jurkat T cells in response to Toxoplasma gondii and LPS. IL-12 and IL-23 production was significantly increased in T cells after stimulation with T. gondii or LPS. T. gondii and LPS increased the phosphorylation of AKT, ERK1/2, p38 MAPK, and JNK1/2 in T cells from 10 min post-stimulation, and peaked at 30-60 min...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29320815/comparison-of-placenta-pcr-and-maternal-serology-of-aborted-women-for-detection-of-toxoplasma-gondii-in-ardabil-iran
#8
Somaie Matin, Gholamreza Shahbazi, Shervin Tabrizian Namin, Rouhallah Moradpour, Farideh Feizi, Hadi Piri-Dogahe
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29319244/seroprevalence-of-toxoplasma-gondii-among-pregnant-women-in-northeast-india
#9
Biswajyoti Borkakoty, Dipankar Biswas, Aniruddha Jakharia, Jagadish Mahanta
Objective: Toxoplasma gondii infection is primarily asymptomatic and one third of world's population is estimated to be infected by this protozoan parasite. This study was carried out to determine the seroprevalence of T. gondii infection among pregnant women from north east India, where data on this important parasitic infection is scanty. Methods: A total of 1141 serum archival samples collected from antenatal clinic attendees in 2007-09, were screened for T. gondii IgG by ELISA and analyzed with their socio demographic information...
October 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/29317880/prevalence-of-toxoplasma-gondii-infection-among-healthy-blood-donors-in-northeast-of-iran
#10
Saeed Sadooghian, Hossein Mahmoudvand, Mohammad Ali Mohammadi, Naser Nazari Sarcheshmeh, Amir Tavakoli Kareshk, Hossein Kamiabi, Naser Zia-Ali
Background: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti-Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015. Methods: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti-T. gondii antibodies by enzyme-linked immunosorbent assay (ELISA)...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29317874/molecular-cloning-expression-and-characterization-of-plasmid-encoding-rhomboid-4-rom4-of-tachyzoite-of-toxoplasma-gondii-rh-strain
#11
Mohammad Taghi Rahimi, Shahabeddin Sarvi, Mahdi Sharif, Saeid Abediankenari, Ehsan Ahmadpour, Reza Valadan, Ahmad Daryani
Background: The objective of this study was to clone, express and characterize the gene encoding rhomboid 4 (ROM4) proteins, a vital gene in surface adhesion and host cell invasion process of tachyzoite of T. gondii in an appropriate expression vector and eukaryotic cell for production of recombinant protein. Methods: Toxoplasma RNA was isolated from tachyzoites (RH strain) and complementary DNA was synthesized. Oligonucleotide primer pair was designed based on Toxoplasma ROM4 gene sequence with XhoI and EcoRI restriction sites at 5' end of forward and reverse primers, respectively...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29317509/human-placental-syncytiotrophoblasts-restrict-toxoplasma-gondii-attachment-and-replication-and-respond-to-infection-by-producing-immunomodulatory-chemokines
#12
Stephanie E Ander, Elizabeth N Rudzki, Nitin Arora, Yoel Sadovsky, Carolyn B Coyne, Jon P Boyle
Toxoplasma gondii is a major source of congenital disease worldwide, but the cellular and molecular factors associated with its vertical transmission are largely unknown. In humans, the placenta forms the key interface between the maternal and fetal compartments and forms the primary barrier that restricts the hematogenous spread of microorganisms. Here, we utilized primary human trophoblast (PHT) cells isolated from full-term placentas and human midgestation chorionic villous explants to determine the mechanisms by which human trophoblasts restrict and respond to T...
January 9, 2018: MBio
https://www.readbyqxmd.com/read/29317472/failure-of-systemic-oral-doxycycline-in-preventing-ocular-toxoplasmic-retinochoroiditis-in-french-military-personnel
#13
Sami Saad, M Delbarre, R Saad, M Berguiga, D Benisty, M Marechal, T Adam, F Froussart
This paper describes two cases of toxoplasmic chorioretinitis presenting in two French soldiers who had been receiving oral doxycycline for malaria prophylaxis. This is despite the proven effectiveness of oral doxycycline in treating Toxoplasma gondii, the most common cause of this infection. The lack of effectiveness of oral doxycycline in these two cases most likely reflected that the ocular concentration of 100 mg daily doxycycline is too low to treat or prevent Toxoplasmic retinochoroiditis (TC). Clinicians should therefore be aware that soldiers taking prophylactic oral doxycycline are still at risk of developing ocular TC with potentially sight-threatening consequences if not treated adequately...
January 8, 2018: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/29315981/distribution-and-processing-of-eimeria-nieschulzi-owp13-a-new-protein-of-the-cowp-family
#14
Stefanie Wiedmer, Ulrike Buder, Sinja Bleischwitz, Michael Kurth
Eimeria species are veterinary important coccidian parasites and are transmitted between hosts via oocysts. The infectious sporozoites are protected by the oocyst and sporocyst wall. Tyrosine-rich proteins are well-known components of the Eimeria oocyst wall. Recently, cysteine motif containing proteins (COWP family), as described in Toxoplasma gondii and Cryptosporidium spp., have also been characterized in Eimeria. Here, we identified a novel COWP related protein, EnOWP13, and tracked it via transfection technology in Eimeria nieschulzi...
January 9, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29315653/group-1-innate-lymphoid-cells-in-toxoplasma-gondii-infection
#15
Ildiko Rita Dunay, Andreas Diefenbach
Innate lymphoid cells (ILCs) are a group of lymphocytes that carry out important functions in immunity to infections and in organ homeostasis at epithelial barrier surfaces. ILCs are innate immune cells that provide an early source of cytokines to initiate immune responses against pathogens. Cytotoxic ILCs (i.e., conventional (c)NK cells) and several subsets of helper-like ILCs are the major brances of the ILC family. Conventional NK cells and group 1 ILCs share several functions such as surface receptors and the ability to produce IFN-γ upon activation but they differ in their developmental paths and in their dependence on specific transcription factors...
January 9, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/29303684/patterns-of-exposure-of-carnivores-to-selected-pathogens-in-the-betampona-natural-reserve-landscape-madagascar
#16
Fidisoa Rasambainarivo, Mamy Navalona Andriamihajarivo, Edward Dubovi, Patricia G Parker
Carnivores of Madagascar are at increased risk of extinction due to anthropogenic loss of habitat, hunting, and interactions with introduced carnivores. Interactions between introduced and native animals also present the potential for introduction of pathogens into new geographic areas or host species. Here, we provide serologic data regarding pathogen exposure of domestic and native carnivores from the Betampona Natural Reserve Landscape, a protected area in eastern Madagascar. For the Eupleridae, we found limited evidence of exposure to viruses from domestic animals but greater prevalence for Toxoplasma gondii (39%) and Leptospira interrogans (40%)...
January 5, 2018: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/29299685/toxoplasma-gondii-infection-and-biosecurity-levels-in-fattening-pigs-and-sows-serological-and-molecular-epidemiology-in-the-intensive-pig-industry-lombardy-northern-italy
#17
Alessia Libera Gazzonis, Marianna Marangi, Luca Villa, Maria Elena Ragona, Emanuela Olivieri, Sergio Aurelio Zanzani, Annunziata Giangaspero, Maria Teresa Manfredi
Toxoplasma gondii is a widespread protozoan affecting animals and humans. One of the major routes of human infection is through the consumption of raw or under-cooked meat, particularly of certain animal species, including pigs. Although T. gondii represents an important public health issue, its control at slaughter is not mandatory. Consequently, available information on T. gondii infection in domestic animals destined for human consumption is scarce. Thus, an epidemiologic survey was designed to update information on T...
January 3, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29297501/t-bet-independent-th1-response-induces-intestinal-immunopathology-during-toxoplasma-gondii-infection
#18
Américo H López-Yglesias, Elise Burger, Alessandra Araujo, Andrew T Martin, Felix Yarovinsky
Coordinated production of IFN-γ by innate and adaptive immune cells is central for host defense, but can also trigger immunopathology. The investigation of the lymphoid cell-specific contribution to the IFN-γ-mediated intestinal pathology during Toxoplasma gondii infection identified CD4+ T cells as a key cell population responsible for IFN-γ-dependent intestinal inflammation and Paneth cell loss, where T-bet-dependent group 1 innate lymphoid cells have a minor role in driving the parasite-induced immunopathology...
January 3, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/29296260/evaluation-of-immune-responses-induced-by-gra7-and-rop2-genes-by-dna-vaccine-cocktails-against-acute-toxoplasmosis-in-balb-c-mice
#19
Hossein Vazini, Fatemeh Ghafarifar, Zohreh Sharifi, Abdolhosein Dalimi
Background: The severe damages of toxoplasmosis clearly indicate the need for the development of a more effective vaccine. Immunization with plasmid DNA is a promising vaccination technique. Therefore, GRA7 plasmid was prepared to be used as a vaccine. The purpose of this study was evaluation of immunization with cocktail DNA vaccine including plasmids encoding Toxoplasma gondii ROP2 and GRA7 in BALB/c mice. Methods: In this study, 733 bp of GRA7 gene was cloned in pCDNA3...
January 2018: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29295815/toxoplasma-gondii-disrupts-%C3%AE-1-integrin-signaling-and-focal-adhesion-formation-during-monocyte-hypermotility
#20
Joshua H Cook, Norikiyo Ueno, Melissa B Lodoen
The motility of blood monocytes is orchestrated by the activity of cell surface integrins, which translate extracellular signals into cytoskeletal changes to mediate adhesion and migration. Toxoplasma gondii is an intracellular parasite that infects migratory cells and enhances their motility, but the mechanisms underlying T. gondii-induced hypermotility are incompletely understood. We have investigated the molecular basis for the hypermotility of primary human peripheral blood monocytes and THP-1 cells infected with T...
January 2, 2018: Journal of Biological Chemistry
keyword
keyword
45270
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"