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Shuting Liu, Boris Wawrik, Zhanfei Liu
Proteins and peptides are key components of the labile dissolved organic matter pool in marine environments. Knowing which types of bacteria metabolize peptides can inform the factors that govern peptide decomposition and further carbon and nitrogen remineralization in marine environments. A (13)C-labeled tetrapeptide, alanine-valine-phenylalanine-alanine (AVFA), was added to both surface (normoxic) and bottom (hypoxic) seawater from a coastal station in the northern Gulf of Mexico for a 2-day incubation experiment, and bacteria that incorporated the peptide were identified using DNA stable isotope probing (SIP)...
2017: Frontiers in Microbiology
Si Luo, Langlang Li, Anwei Chen, Qingru Zeng, Hao Xia, Ji-Dong Gu
In this study, the dibutyl phthalate (DBP) binding properties of a DBP-tolerant bacterium (B. cepacia) were characterized in terms of adsorption kinetics and isotherm. Living and nonliving cells both exhibited rapid removal of DBP, achieving more than 80% of maximum sorption within 30 min of contact and reached the equilibrium after 3 h. The adsorption isotherms were well fitted with the Sips model and the nonliving cells have greater biosorption capacity and affinity for DBP than the living cells. Furthermore, the absence of an active mechanism dependent on metabolism implied that the DBP bioaccumulation by living cells was mainly attribute to passive surface binding...
March 11, 2017: Chemosphere
Lisa T C M van Weert, Jacobus C Buurstede, Ahmed Mahfouz, Pamela S M Braakhuis, J Annelies E Polman, Hetty C M Sips, Benno Roozendaal, Judit Balog, E Ronald de Kloet, Nicole A Datson, Onno C Meijer
In the limbic brain, mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs) both function as receptors for the naturally occurring glucocorticoids (corticosterone/cortisol), but mediate distinct effects on cellular physiology via transcriptional mechanisms. The transcriptional basis for specificity of these MR- versus GR-mediated effects is unknown. To address this conundrum we have identified the extent of MR/GR DNA binding selectivity in the rat hippocampus using chromatin immunoprecipitation followed by sequencing (ChIP-seq)...
January 24, 2017: Endocrinology
Xiaolei Li, Xiaomei Xie, Hanhan Luo, Lan Li, Zhuang Li, Zhiyong Xue, Wei Li
Cucurbit[8]uril (CB[8]) and cucurbit[6]uril (CB[6]) were applied as a high efficiency absorbent to remove reactive yellow X-RG and reactive brilliant red X-3B dyes in aqueous solution. Factors affecting adsorption such as dosage, contact time, temperature, pH value and metal cation were investigated. The adsorption isotherms for X-RG followed the Langmuir and Sips model while for X-3B followed the Freundlich model. Adsorption thermodynamic showed that X-RG and X-3B adsorption onto CB[8] and CB[6] was a spontaneous and enthalpy-driven process...
March 10, 2017: Journal of Colloid and Interface Science
Juliane Menting, Cees J Tack, Hans Knoop
OBJECTIVE: To investigate the prevalence, location and severity of pain, as well as its association with psychosocial and clinical variables and its impact on functional impairment in fatigued patients with type 1 diabetes. METHODS: 120 severely fatigued patients with type 1 diabetes completed questionnaires on pain (McGill Pain Questionnaire, MPQ; Short Form Health Survey subscale bodily pain, SF-36), fatigue severity (Checklist Individual Strength subscale fatigue severity, CIS), depressive symptoms (Beck Depression Inventory Primary Care, BDI-PC) and functional impairment (Sickness Impact Profile-8, SIP-8)...
April 2017: Journal of Psychosomatic Research
Elena Herrmann, Wayne Young, Douglas Rosendale, Verena Reichert-Grimm, Christian U Riedel, Ralf Conrad, Markus Egert
RNA-based stable isotope probing (RNA-SIP) and metabolic profiling were used to detect actively glucose-consuming bacteria in a complex microbial community obtained from a murine model system. A faeces-derived microbiota was incubated under anaerobic conditions for 0, 2, and 4 h with 40 mM [U(13)C]glucose. Isopycnic density gradient ultracentrifugation and fractionation of isolated RNA into labeled and unlabeled fractions followed by 16S rRNA sequencing showed a quick adaptation of the bacterial community in response to the added sugar, which was dominated by unclassified Lachnospiraceae species...
2017: BioMed Research International
Blair G Paul, Haibing Ding, Sarah C Bagby, Matthias Y Kellermann, Molly C Redmond, Gary L Andersen, David L Valentine
The marine subsurface is a reservoir of the greenhouse gas methane. While microorganisms living in water column and seafloor ecosystems are known to be a major sink limiting net methane transport from the marine subsurface to the atmosphere, few studies have assessed the flow of methane-derived carbon through the benthic mat communities that line the seafloor on the continental shelf where methane is emitted. We analyzed the abundance and isotope composition of fatty acids in microbial mats grown in the shallow Coal Oil Point seep field off Santa Barbara, CA, USA, where seep gas is a mixture of methane and CO2...
2017: Frontiers in Microbiology
Jéssica A Paes, Veridiana G Virginio, Martín Cancela, Fernanda M A Leal, Thiago J Borges, Natália Jaeger, Cristina Bonorino, Irene S Schrank, Henrique B Ferreira
Mycoplasma hyopneumoniae is an economically significant swine pathogen that causes porcine enzootic pneumonia (PEP). Important processes for swine infection by M. hyopneumoniae depend on cell surface proteins, many of which are secreted by secretion pathways not completely elucidated so far. A putative type I signal peptidase (SPase I), a possible component of a putative Sec-dependent pathway, was annotated as a product of the sipS gene in the pathogenic M. hyopneumoniae 7448 genome. This M. hyopneumoniae putative SPase I (MhSPase I) displays only 14% and 23% of sequence identity/similarity to Escherichia coli bona fide SPase I, and, in complementation assays performed with a conditional E...
March 2017: Veterinary Microbiology
Agnieszka M Topolska-Woś, Sara Rosińska, Anna Filipek
Mitogen-activated protein (MAP) kinases are important players in cellular signaling pathways. Recently, it has been shown that CacyBP/SIP serves as a phosphatase for one of the MAP kinases, ERK1/2. Through dephosphorylation of this kinase CacyBP/SIP modulates the transcriptional activity of Elk-1 and the activity of the CREB-BDNF pathway. In this work, using NB2a cell lysate and recombinant proteins, we show that CacyBP/SIP binds and dephosphorylates another member of the MAP kinase family, p38. Analysis of recombinant full-length CacyBP/SIP and its three major domains, N-terminal, middle CS and C-terminal SGS, indicates that the middle CS domain is responsible for p38 dephosphorylation...
March 10, 2017: Amino Acids
Sonia Sharmin, Kypros Kypri, Masuma Khanam, Monika Wadolowski, Raimondo Bruno, Richard P Mattick
Whether parental supply of alcohol affects the likelihood of later adolescent risky drinking remains unclear. We conducted a systematic review and meta-analysis to synthesize findings from longitudinal studies investigating this association. We searched eight electronic databases up to 10 September 2016 for relevant terms and included only original English language peer-reviewed journal articles with a prospective design. Two reviewers independently screened articles, extracted data and assessed risk of bias...
March 9, 2017: International Journal of Environmental Research and Public Health
A Merchán, S V Navarro, A B Klein, S Aznar, L Campa, C Suñol, M Moreno, P Flores
RATIONALE: Compulsive behaviour, present in different psychiatric disorders, such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of monoamines, particularly serotonin (5-hydroxytryptamine) and its receptor system. OBJECTIVES: The present study investigated whether 5-HT manipulation, through a tryptophan (TRP) depletion by diet in Wistar and Lister Hooded rats, modulates compulsive drinking in schedule-induced polydipsia (SIP) and locomotor activity in the open-field test...
March 9, 2017: Psychopharmacology
Liliana Matos, Alexandra Monteiro Gouveia, Henrique Almeida
Copper sulfate-induced premature senescence (CuSO4-SIPS) consistently mimetized molecular mechanisms of replicative senescence, particularly at the endoplasmic reticulum proteostasis level. In fact, disruption of protein homeostasis has been associated to age-related cell/tissue dysfunction and human disorders susceptibility. Resveratrol is a polyphenolic compound with proved antiaging properties under particular conditions. In this setting, we aimed to evaluate resveratrol ability to attenuate cellular senescence induction and to unravel related molecular mechanisms...
2017: Oxidative Medicine and Cellular Longevity
Tero Luukkonen, Kateřina Věžníková, Emma-Tuulia Tolonen, Hanna Runtti, Juho Yliniemi, Tao Hu, Kimmo Kemppainen, Ulla Lassi
Ammonium (NH4(+)) removal from municipal wastewater poses challenges with the commonly used biological processes. Especially at low wastewater temperatures, the process is frequently ineffective and difficult to control. One alternative is to use ion-exchange. In the present study, a novel NH4(+) ion-exchanger, metakaolin geopolymer (MK-GP), was prepared, characterized, and tested. Batch experiments with powdered MK-GP indicated that the maximum exchange capacities were 31.79, 28.77, and 17.75 mg/g in synthetic, screened, and pre-sedimented municipal wastewater, respectively, according to the Sips isotherm (R(2) ≥ 0...
March 1, 2017: Environmental Technology
Eva Malatinkova, Ward De Spiegelaere, Linos Vandekerckhove, Magdalena Sips
The HIV Cure Research Center (HCRC) in Ghent organised the first HIV Reservoir Characterization Symposium, and brought together virologists, molecular biologists, immunologists and clinicians to discuss the most recent developments in HIV reservoir characterisation with a view to achieving an HIV cure. The one-day symposium covered new developments in the field of HIV reservoir and HIV cure research, with the latest news on the European HIV cure trials. This report summarises the major themes discussed during the symposium...
January 1, 2017: Journal of Virus Eradication
Yi-Peng Gu, Xiao-Mei Yang, Ping Luo, Yan-Qun Li, Ye-Xing Tao, Zhen-Hua Duan, Wei Xiao, Da-Yan Zhang, Hua-Zhong Liu
In our recent reports, a squid ink polysaccharide (SIP) was found having preventive activity against cyclophosphamide induced damage in mouse testis and ovary. Here we further reveal the regulative mechanism of SIP against chemical toxicity on testis. Leydig cells exposed to acrolein (ACR) underwent apoptosis at 12h and 24h. Before apoptosis, cells occurred autophagy that was confirmed by high autophagic rate and Beclin-1 protein content at 3h. PI3K/Akt and p38 MAPK signal pathways involved in the regulatory mechanisms...
May 1, 2017: Carbohydrate Polymers
Daewoo Han, Xinjun Yu, Qinyuan Chai, Neil Ayres, Andrew J Steckl
The first self-immolative polymer (SIP) nanofiber membrane have been demonstrated in this report, which the immolation can be triggered by external stimulus. Electrospun SIP/PAN fibers provide ~ 25 times quicker and more responsive depolymerization (i.e. immolation) than a cast film in the triggering condition. Depolymerization of SIP in the SIP/PAN blended fiber membrane results in the transition of the surface properties from hydrophobic (~110º) to hygroscopic (~ 0º). Triggered release of encapsulated functional molecules has been demonstrated using coaxially electrospun fiber membrane made of SIP/PAN blend sheath and PVP/dye core...
March 6, 2017: ACS Applied Materials & Interfaces
Kenichi Ishibashi, Yoshiyuki Morishita, Yasuko Tanaka
Aquaporins (AQPs ) are a family of transmembrane proteins present in almost all species including virus. They are grossly divided into three subfamilies based on the sequence around a highly conserved pore-forming NPA motif: (1) classical water -selective AQP (CAQP), (2) glycerol -permeable aquaglyceroporin (AQGP) and (3) AQP super-gene channel, superaquaporin (SAQP). AQP is composed of two tandem repeats of conserved three transmembrane domains and a NPA motif. AQP ancestors probably started in prokaryotes by the duplication of half AQP genes to be diversified into CAQPs or AQGPs by evolving a subfamily-specific carboxyl-terminal NPA motif...
2017: Advances in Experimental Medicine and Biology
Georgios D Kotzalidis, Andrea Solfanelli, Daria Piacentino, Valeria Savoja, Paolo Fiori Nastro, Martina Curto, Juliana Fortes Lindau, Alice Masillo, Martina Brandizzi, Francesca Fagioli, Andrea Raballo, Eva Gebhardt, Antonio Preti, Marco D'Alema, Maria Rosa Fucci, Roberto Miletto, Daniela Andropoli, Donato Leccisi, Paolo Girardi, Rachel L Loewy, Frauke Schultze-Lutter
BACKGROUND: Current early screeners for psychosis-risk states have still to prove ability in identifying at-risk individuals. Among screeners, the 92-item Prodromal Questionnaire (PQ-92) is often used. We aimed to assess the validity of its Italian translation in a large Italian adolescent and young adult help-seeking sample. METHODS: We included all individuals aged 12-36years seeking help at psychiatric mental health services in a large semirural Roman area (534,600 population) who accepted to participate...
February 18, 2017: Schizophrenia Research
Andreas Oberbach, Sven-Bastiaan Haange, Nadine Schlichting, Marco Heinrich, Stefanie Lehmann, Holger Till, Floor Hugenholtz, Yvonne Kullnick, Hauke Smidt, Karin Frank, Jana Seifert, Nico Jehmlich, Martin von Bergen
The gastrointestinal microbiota in the gut interacts metabolically and immunologically with the host tissue in the contact zone of the mucus layer. For understanding the details of these interactions and especially their dynamics it is crucial to identify the metabolically active subset of the microbiome. This became possible by the development of stable isotope probing techniques, which have only sparsely been applied to microbiome research. We applied the in vivo stable isotope approach using (15)N-labeled diet with subsequent identification of metabolically active bacterial species...
March 21, 2017: Journal of Proteome Research
G Brucato, M D Masucci, L Y Arndt, S Ben-David, T Colibazzi, C M Corcoran, A H Crumbley, F M Crump, K E Gill, D Kimhy, A Lister, S A Schobel, L H Yang, J A Lieberman, R R Girgis
BACKGROUND: DSM-5 proposes an Attenuated Psychosis Syndrome (APS) for further investigation, based upon the Attenuated Positive Symptom Syndrome (APSS) in the Structured Interview for Psychosis-Risk Syndromes (SIPS). SIPS Unusual Thought Content, Disorganized Communication and Total Disorganization scores predicted progression to psychosis in a 2015 NAPLS-2 Consortium report. We sought to independently replicate this in a large single-site high-risk cohort, and identify baseline demographic and clinical predictors beyond current APS/APSS criteria...
March 2, 2017: Psychological Medicine
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