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https://www.readbyqxmd.com/read/28526484/regulation-of-cacybp-sip-expression-by-nfat1-transcription-factor
#1
Beata Kądziołka, Wiesława Leśniak, Anna Filipek
In this work we have shown that NFAT1 transcription factor is involved in the regulation of CacyBP/SIP expression. We have demonstrated, by applying Western blot, RT-PCR and luciferase assay that the level of CacyBP/SIP increases upon NFAT1 overexpression. Moreover, inhibition or stimulation of NFAT transcriptional activity exerts a corresponding effect on the expression of CacyBP/SIP gene. Furthermore, EMSA and chromatin immunoprecipitation (ChIP) assay have shown that NFAT1 binds to its specific binding sites within the CacyBP/SIP gene...
May 11, 2017: Immunobiology
https://www.readbyqxmd.com/read/28511128/improving-the-repeatability-of-motor-unit-number-index-munix-by-introducing-additional-epochs-at-low-contraction-levels
#2
Yun Peng, Yingchun Zhang
OBJECTIVE: To evaluate the repeatability of (Motor Unit Number Index) MUNIX under repeatability conditions, specify the origin of variations and provide strategies for quality control. METHODS: MUNIX calculations were performed on the bicep brachii muscles of eight healthy subjects. Negative effect of suboptimal electrode positions on MUNIX accuracy was eliminated by employing the high-density surface electromyography technique. MUNIX procedures that utilized a variety of surface interferential pattern (SIP) epoch recruitment strategies (including the original MUNIX procedure, two proposed improvement strategies and their combinations) were described...
April 8, 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/28511110/determination-of-regional-relationships-among-salmonella-spp-isolated-from-retail-pork-circulating-in-the-chiang-mai-municipality-area-using-a-wgs-data-approach
#3
Prapas Patchanee, Thanaporn Eiamsam-Ang, Juntakarn Vanaseang, Phacharaporn Boonkhot, Pakpoom Tadee
Salmonella is recognized as a significant zoonotic foodborne pathogen, and pork products are involved in one-fifth of infections. Whole genome sequencing data of Salmonella isolated from retail's pork circulating in the Chiang Mai Municipality area between April 2013 and September 2014, were used to focus on genetic diversity and proven in pig-human transmission based on Multilocus Sequence Typing (MLST). Additionally, WGS data were used to investigate virulence genes, to assess the hazard or pathogenic potential transferred into the food production chain...
May 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28505027/automated-surveillance-of-healthcare-associated-infections-state-of-the-art
#4
Meander E Sips, Marc J M Bonten, Maaike S M van Mourik
PURPOSE OF REVIEW: This review describes recent advances in the field of automated surveillance of healthcare-associated infections (HAIs), with a focus on data sources and the development of semiautomated or fully automated algorithms. RECENT FINDINGS: The availability of high-quality data in electronic health records and a well-designed information technology (IT) infrastructure to access these data are indispensable for successful implementation of automated HAI surveillance...
May 12, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28495344/does-adoption-of-electronic-health-records-improve-the-quality-of-care-management-in-france-results-from-the-french-e-si-preps-sips-study
#5
Morgane Plantier, Nathalie Havet, Thierry Durand, Nicolas Caquot, Camille Amaz, Pierre Biron, Irène Philip, Lionel Perrier
BACKGROUND: Electronic health records (EHR) are increasingly being adopted by healthcare systems worldwide. In France, the "Hôpital numérique 2012-2017" program was implemented as part of a strategic plan to modernize health information technology (HIT), including the promotion of widespread EHR use. With significant upfront investment costs as well as ongoing operational expenses, it is important to assess this system in terms of its ability to result in improvements in hospital performances...
June 2017: International Journal of Medical Informatics
https://www.readbyqxmd.com/read/28482053/a-four-session-sleep-intervention-program-improves-sleep-for-older-adult-day-health-care-participants-results-of-a-randomized-controlled-trial
#6
Jennifer L Martin, Yeonsu Song, Jaime Hughes, Stella Jouldjian, Joseph M Dzierzewski, Constance H Fung, Juan Carlos Rodriguez Tapia, Michael N Mitchell, Cathy A Alessi
Study Objective: To test the effectiveness of a 4-week behavioral Sleep Intervention Program (SIP: sleep compression, modified stimulus control, and sleep hygiene) compared to a 4-week information-only control (IC) among older adults attending a VA Adult Day Health Care (ADHC) program in a double-blind, randomized, clinical trial. Methods: 42 individuals (mean age: 77 years, 93% male) enrolled in a VA ADHC program were randomized to receive SIP or IC. All completed in-person sleep and health assessments at baseline, post-treatment and 4-months follow-up that included 3 days/nights of wrist actigraphy, the Pittsburgh Sleep Quality Index (PSQI), and the Insomnia Severity Index (ISI)...
May 6, 2017: Sleep
https://www.readbyqxmd.com/read/28478937/coexpression-of-growth-differentiation-factor-11-and-reactive-oxygen-species-in-metastatic-oral-cancer-and-its-role-in-inducing-the-epithelial-to-mesenchymal-transition
#7
Xiangming Qin, Hai Kuang, Lei Chen, Shanliang Wei, Dahai Yu, Feixin Liang
OBJECTIVES: The aim of this study was to investigate growth differentiation factor 11 (GDF11) and reactive oxygen species (ROS) expression in metastatic oral cancer and explored their roles in inducing epithelial to mesenchymal transition (EMT). STUDY DESIGN: The expression of GDF11, ROS, and EMT-related markers was evaluated in primary tumor tissues from patients with oral squamous cell carcinoma (OSCC). SCC-9 cells, a human tongue carcinoma cell line, were treated with recombinant GDF11...
June 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28476676/protective-efficacy-of-cationic-plga-microspheres-loaded-with-dna-vaccine-encoding-the-sip-gene-of-streptococcus-agalactiae-in-tilapia
#8
Yan-Ping Ma, Hao Ke, Zhi-Ling Liang, Jiang-Yao Ma, Le Hao, Zhen-Xing Liu
Streptococcus agalactiae (S. agalactiae) is an important fish pathogen, which has received more attention in the past decade due to the increasing economic losses in the tilapia industry worldwide. As existing effective vaccines of S. agalactiae in fish have obvious disadvantage, to select immunoprotective antigens and package materials would undoubtedly contribute to the development of novel oral vaccines. In the present study, surface immunogenic protein (sip) was selected from the S. agalactiae serovar I a genomes as immunogenic protein in DNA vaccine form with cationic chitosan and biodegradable and biocompatible PLGA...
May 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28471280/bad-trip-due-to-25i-nbome-a-case-report-from-the-eu-project-spice-ii-plus
#9
Maren Hermanns-Clausen, Verena Angerer, Josephine Kithinji, Christina Grumann, Volker Auwärter
OBJECTIVE: The potent hallucinogenic drug 25I-NBOMe has recently emerged on the drug market. We present a case with analytically confirmed 25I-NBOMe intoxication from the prospective study "SPICE II Plus". CASE REPORT: Because of a severe headache a 42-year-old man took one sip of a pediatric analgesic syrup, which had been refilled with a self-made solution of 25I-NBOMe in ethanol. Thirty minutes later restlessness occurred. On arrival in the emergency department mydriasis, strong sweating, disorientation, and agitation were noticed...
May 4, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28471019/type-and-rate-of-atrial-fibrillation-termination-due-to-rotational-activity-ablation-combined-with-pulmonary-vein-isolation
#10
Zsuzsanna Kis, Dominic A Theuns, Rohit Bhagwandien, Sip Wijchers, Sing-Chien Yap, Tamas Szili-Torok
BACKGROUND: There remains controversy about the optimal ablation technique and termination rate of atrial fibrillation (AF) during catheter ablation in patients with persistent AF. The aim of this study was to determine the rate and timing of AF termination during combined pulmonary vein isolation (PVI) and focal impulse and rotor modulation (FIRM)-guided ablation of rotational activity (RoAc). METHODS: This single-center, prospective cohort study enrolled 38 consecutive patients (63% male, mean age 63 ± 11 years) with persistent AF (mean left atrial size: 46 ± 7 mm), who underwent FIRM mapping and ablation of the identified RoAcs followed by PVI...
May 4, 2017: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/28468896/emergency-laparotomy-in-infants-born-at-26-weeks-gestation-a-neonatal-network-based-cohort-study-of-frequency-surgical-pathology-and-outcomes
#11
Jonathan Durell, Nigel J Hall, Melanie Drewett, Kujan Paramanantham, David Burge
OBJECTIVE: Identify the proportion of infants born at <26 completed weeks' gestation who require emergency laparotomy, and review the surgical pathology, incidence of subsequent surgical procedures and outcome. DESIGN: Retrospective cohort review. SETTING: Tertiary neonatal surgical unit. PATIENTS: All infants born at <26 weeks' gestation in a neonatal network over an 8-year period. RESULTS: Of 381 infants, laparotomy was indicated in 61 (16%) and performed in 57...
May 3, 2017: Archives of Disease in Childhood. Fetal and Neonatal Edition
https://www.readbyqxmd.com/read/28464099/endogenous-phosphorus-losses-in-growing-finishing-pigs-and-gestating-sows
#12
P Bikker, C M C van der Peet-Schwering, W J J Gerrits, V Sips, C Walvoort, H van Laar
An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows. After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement. Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2...
April 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28456206/benchmark-coupled-cluster-g-tensor-calculations-with-full-inclusion-of-the-two-particle-spin-orbit-contributions
#13
Ajith Perera, Jürgen Gauss, Prakash Verma, Jorge A Morales
We present a parallel implementation to compute electron spin resonance g-tensors at the coupled-cluster singles and doubles (CCSD) level which employs the ACES III domain-specific software tools for scalable parallel programming, i.e., the super instruction architecture language and processor (SIAL and SIP), respectively. A unique feature of the present implementation is the exact (not approximated) inclusion of the five one- and two-particle contributions to the g-tensor [i.e., the mass correction, one- and two-particle paramagnetic spin-orbit, and one- and two-particle diamagnetic spin-orbit terms]...
April 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28455101/sip-1-mutations-cause-disturbances-in-the-activity-of-nmda-and-ampa-but-not-kainate-receptors-of-neurons-in-the-cerebral-cortex
#14
Maria V Turovskaya, Alexei A Babaev, Valery P Zinchenko, Ekaterina A Epifanova, Ekaterina V Borisova, Victor S Tarabykin, Egor A Turovsky
Smad-interacting protein-1 (Sip1) [Zinc finger homeobox (Zfhx1b), Zeb2] is a transcription factor implicated in the genesis of Mowat-Wilson syndrome (MWS) in humans. MWS is a rare genetic autosomal dominant disease caused by a mutation in the Sip1 gene (aka Zeb2 or Zfhx1b) mapped to 2q22.3 locus. MWS affects 1 in every 50-100 newborns worldwide. It is characterized by mental retardation, small stature, typical facial abnormalities as well as disturbances in the development of the cardio-vascular and renal systems as well as some other organs...
April 25, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28452221/surface-enhanced-raman-spectroscopy-combined-with-stable-isotope-probing-to-monitor-nitrogen-assimilation-at-both-bulk-and-single-cell-level
#15
Li Cui, Kai Yang, Guowei Zhou, Wei E Huang, Yong-Guan Zhu
Microbe-mediated biogeochemical cycle of nitrogen is a critical process in the environment. In this study, surface-enhanced Raman spectroscopy combined with (15)N stable isotope probing (SERS-(15)N SIP) was developed as a new, nondestructive, and robust approach to probe nitrogen assimilation by bacteria at both bulk and single-cell level, and from pure culture to environmental microbial community. Multiple distinguishable SERS band shifts were observed and displayed a linear relationship with (15)N content, because of the substitution of "light" nitrogen by "heavier" (15)N stable isotope...
May 8, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28452061/evaluation-of-real-life-dosing-of-oral-medicines-with-respect-to-fluid-and-food-intake-in-a-dutch-speaking-population
#16
B Hens, J Van Den Abeele, J Rubbens, M Keirsebilck, J Roelens, C Schreurs, K Verheyen, M Casteels, G Laekeman, P Augustijns
WHAT IS KNOWN AND OBJECTIVE: Oral drug administration is the most preferred route of drug administration. For some specific classes of drugs, recommendations regarding the intake of the drug product are provided by and approved in the summary of product characteristics (SmPC) after testing the oral drug product in clinical trials under strict and predefined conditions. The aim of this study was to investigate how certain classes of medicines are taken in a "real-life" setting in terms of concomitant fluid and food intake by a Dutch-speaking population in Flanders (Belgium)...
April 27, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28447607/genome-wide-identification-characterization-and-expression-profile-of-aquaporin-gene-family-in-flax-linum-usitatissimum
#17
S M Shivaraj, Rupesh K Deshmukh, Rhitu Rai, Richard Bélanger, Pawan K Agrawal, Prasanta K Dash
Membrane intrinsic proteins (MIPs) form transmembrane channels and facilitate transport of myriad substrates across the cell membrane in many organisms. Majority of plant MIPs have water transporting ability and are commonly referred as aquaporins (AQPs). In the present study, we identified aquaporin coding genes in flax by genome-wide analysis, their structure, function and expression pattern by pan-genome exploration. Cross-genera phylogenetic analysis with known aquaporins from rice, arabidopsis, and poplar showed five subgroups of flax aquaporins representing 16 plasma membrane intrinsic proteins (PIPs), 17 tonoplast intrinsic proteins (TIPs), 13 NOD26-like intrinsic proteins (NIPs), 2 small basic intrinsic proteins (SIPs), and 3 uncharacterized intrinsic proteins (XIPs)...
April 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28443162/novel-measurement-technique-for-the-sagittal-vertical-axis-and-its-clinical-application-in-adult-spinal-deformity
#18
Go Yoshida, Kenta Kurosu, Yu Yamato, Tomohiko Hasegawa, Tatsuya Yasuda, Daisuke Togawa, Yukihiro Matsuyama
STUDY DESIGN: Prospective physical measurement of the sagittal vertical axis (SVA). PURPOSE: To evaluate a simple method for measuring SVA by analyzing its relationship with radiographic measurements and clinical appearance. OVERVIEW OF LITERATURE: No studies have examined physical measurements using the cranial center of gravity (CCG) in a relaxed standing position. METHODS: The physical measurement of the horizontal distance between CCG and spina iliaca posterior superior (CCG-SIPS) was measured using a straight ruler in 252 healthy volunteers and 56 patients with adult spinal deformity...
April 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/28421830/sinonasal-inverted-papilloma-risk-factors-for-local-recurrence-after-surgical-resection
#19
Quentin Lisan, Ollivier Laccourreye, Pierre Bonfils
OBJECTIVES: Sinonasal inverted papillomas (SIP) present a potential for recurrence years after the surgery, but most studies report short-term follow-up, and risk factors for recurrence are still debated. Furthermore, several classifications are described, and no consensus exists regarding which one should be used. The aims of this study were to report our long-term results, investigate for potential risk factors for recurrence, and compare the existing 8 staging systems. METHODS: Over a 28-year period, 110 patients with a diagnosis of SIP were enrolled...
June 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28419925/characterization-and-application-of-restricted-access-carbon-nanotubes-in-online-extraction-of-anticonvulsant-drugs-from-plasma-samples-followed-by-liquid-chromatography-analysis
#20
Rodrigo Campos Dos Santos, Adriana Kaori Kakazu, Mariane Gonçalves Santos, Fábio Antônio Belinelli Silva, Eduardo Costa Figueiredo
Anticonvulsant drugs are often used in the treatment of epilepsy. However, their therapeutic monitoring is often necessary in order to obtain an appropriate dose adjustment, due to the proximity between their therapeutic and toxic ranges. The aim of this study was to carry out the synthesis, characterization and use of restricted access carbon nanotubes (RACNTs) in an online method for the analyses of phenobarbital and carbamazepine and primidone from untreated human blood plasma by column switching liquid chromatography...
April 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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