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https://www.readbyqxmd.com/read/28806761/transcriptome-analysis-of-pdgfr%C3%AE-cells-identifies-t-type-ca2-channel-cacna1g-as-a-new-pathological-marker-for-pdgfr%C3%AE-cell-hyperplasia
#1
Se Eun Ha, Moon Young Lee, Masaaki Kurahashi, Lai Wei, Brian G Jorgensen, Chanjae Park, Paul J Park, Doug Redelman, Kent C Sasse, Laren S Becker, Kenton M Sanders, Seungil Ro
Platelet-derived growth factor receptor alpha (PDGFRα)+ cells are distributed into distinct morphological groups within the serosal, muscular, and submucosal layers as well as the myenteric and deep muscular plexi. PDGFRα+ cells directly interact with interstitial cells of Cajal (ICC) and smooth muscle cells (SMC) in gastrointestinal smooth muscle tissue. These three cell types, SMC, ICC, and PDGFRα+ cells (SIP cells), form an electrical syncytium, which dynamically regulates gastrointestinal motility. We have previously reported the transcriptomes of SMC and ICC...
2017: PloS One
https://www.readbyqxmd.com/read/28804980/using-bayes-factors-to-evaluate-evidence-for-no-effect-examples-from-the-sips-project
#2
Zoltan Dienes, Simon Coulton, Nick Heather
AIMS: To illustrate how Bayes Factors are important for determining the effectiveness of interventions. METHOD: We consider a case where inappropriate conclusions were publicly drawn based on significance testing, namely the SIPS Project (Screening and Intervention Programme for Sensible drinking), a pragmatic, cluster-randomized controlled trial in each of two healthcare settings and in the criminal justice system. We show how Bayes Factors can disambiguate the non-significant findings from the SIPS Project and thus determine whether the findings represent evidence of absence or absence of evidence...
August 13, 2017: Addiction
https://www.readbyqxmd.com/read/28803039/suicidal-behaviors-and-their-relationship-with-psychotic-like-symptoms-in-children-and-adolescents-at-clinical-high-risk-for-psychosis
#3
Eugene J D'Angelo, Sarah Hope Lincoln, Nicholas Morelli, Kelsey Graber, Sahil Tembulkar, Joseph Gonzalez-Heydrich
BACKGROUND: Previous research has demonstrated elevated rates of suicide attempts and ideation in individuals with psychosis. This study investigated rates and severity of suicidal behavior in youth with and at clinical high risk for psychosis, and examined the positive, negative, and disorganized symptoms associated with suicidal behaviors among the clinical high risk group. METHODS: Eighty-six youth ages 7-18 (n=21 non-clinical controls [NCC], n=40 clinical high risk [CHR], n=25 diagnosed psychotic disorder [PD]) were recruited...
July 20, 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/28802824/highly-accurate-prediction-of-protein-self-interactions-by-incorporating-the-average-block-and-pssm-information-into-the-general-pseaac
#4
Jing-Xuan Zhai, Tian-Jie Cao, Ji-Yong An, Yong-Tao Bian
It is a challenging task for fundamental research whether proteins can interact with their partners. Protein self-interaction (SIP) is a special case of PPIs, which plays a key role in the regulation of cellular functions. Due to the limitations of experimental self-interaction identification, it is very important to develop an effective biological tool for predicting SIPs based on protein sequences. In the study, we developed a novel computational method called RVM-AB that combines the Relevance Vector Machine (RVM) model and Average Blocks (AB) for detecting SIPs from protein sequences...
August 9, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28800138/phylogenetically-conserved-resource-partitioning-in-the-coastal-microbial-loop
#5
Samuel Bryson, Zhou Li, Francisco Chavez, Peter K Weber, Jennifer Pett-Ridge, Robert L Hettich, Chongle Pan, Xavier Mayali, Ryan S Mueller
Resource availability influences marine microbial community structure, suggesting that population-specific resource partitioning defines discrete niches. Identifying how resources are partitioned among populations, thereby characterizing functional guilds within the communities, remains a challenge for microbial ecologists. We used proteomic stable isotope probing (SIP) and NanoSIMS analysis of phylogenetic microarrays (Chip-SIP) along with 16S rRNA gene amplicon and metagenomic sequencing to characterize the assimilation of six (13)C-labeled common metabolic substrates and changes in the microbial community structure within surface water collected from Monterey Bay, CA...
August 11, 2017: ISME Journal
https://www.readbyqxmd.com/read/28799925/investigation-of-phosphate-removal-from-aqueous-solution-by-both-coal-gangues
#6
Wei Ding, Shuqin Bai, Haorong Mu, Gaowa Naren
Equilibrium studies were carried out for the adsorption of phosphate onto newly discharged coal gangue and spontaneous combustion coal gangue, which are industrial solid residues. The experimental data were fitted to the two-parameter equations of Freundlich, Langmuir, Temkin, and Dubinin-Radushkevich and the three-parameter equations of the Redlich-Peterson, Sips and Toth isotherms by non-linear method. All three-parameter isotherm equations have a higher correlation coefficient than the two-parameter isotherm equations...
August 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28795812/investigation-of-the-adsorption-of-amphipathic-macroraft-agents-onto-montmorillonite-clay
#7
Rodrigo Duarte Silva, Igor Stefanichen Monteiro, Thaïssa De Camargo Chaparro, Raíssa Silva Hardt, Reinaldo Giudici, Ana Barros-Timmons, Elodie Bourgeat-Lami, Amilton Martins Dos Santos
Recently, there has been significant interest in the use of the reversible addition-fragmentation chain-transfer (RAFT) technique to generate a variety of organic/inorganic colloidal composite particles in aqueous dispersed media using the so-called macroRAFT-assisted encapsulating emulsion polymerization (REEP) strategy. In this process, special attention should be paid to the adsorption of the macromolecular RAFT (macroRAFT) agent onto the inorganic particles as it determines the final particle morphology and can also influence latex stability...
August 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28790981/determination-of-resistant-starch-assimilating-bacteria-in-fecal-samples-of-mice-by-in-vitro-rna-based-stable-isotope-probing
#8
Elena Herrmann, Wayne Young, Douglas Rosendale, Ralf Conrad, Christian U Riedel, Markus Egert
The impact of the intestinal microbiota on human health is becoming increasingly appreciated in recent years. In consequence, and fueled by major technological advances, the composition of the intestinal microbiota in health and disease has been intensively studied by high throughput sequencing approaches. Observations linking dysbiosis of the intestinal microbiota with a number of serious medical conditions including chronic inflammatory disorders and allergic diseases suggest that restoration of the composition and activity of the intestinal microbiota may be a treatment option at least for some of these diseases...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28779876/detection-of-streptococcus-agalactiae-by-immunochromatography-with-group-b-streptococcus-specific-surface-immunogenic-protein-in-pregnant-women
#9
Yoko Takayama, Hidehito Matsui, Yuzuru Adachi, Shin Nihonyanagi, Tatsuhiko Wada, Junko Mochizuki, Nobuya Unno, Hideaki Hanaki
BACKGROUND: Infection with Streptococcus agalactiae (Group B streptococcus: GBS) is a significant cause of morbidity and mortality in neonates. Screening for GBS is mainly done by culture-based methods, but a reliable result may take several days to obtain and culture is difficult to perform at institutions without a laboratory. We evaluated an immunochromatography method for rapid detection of GBS-specific surface immunogenic protein (Sip) using anti-Sip monoclonal antibodies. MATERIALS AND METHODS: A total of 377 cervical and vaginal swabs collected during weeks 35-37 of gestation were inoculated into GBS medium F and incubated...
August 2, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28779853/preliminary-psychometric-properties-of-the-brief-negative-symptom-scale-in-youth-at-clinical-high-risk-for-psychosis
#10
Gregory P Strauss, Hannah C Chapman
Preliminary psychometric properties of an adapted version of the Brief Negative Symptom Scale (BNSS) are reported in youth at Clinical High-Risk for psychosis (CHR). Participants included 29 CHR youth who met criteria for a prodromal syndrome on the Structured Interview for Prodromal Syndromes (SIPS). The adapted BNSS demonstrated excellent internal consistency, convergent validity, and discriminant validity, suggesting that the BNSS has utility for assessing negative symptoms in a CHR population.
August 2, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28777987/adsorption-of-phosphate-from-aqueous-solution-using-electrochemically-modified-biochar-calcium-alginate-beads-batch-and-fixed-bed-column-performance
#11
Kyung-Won Jung, Tae-Un Jeong, Jae-Woo Choi, Kyu-Hong Ahn, Sang-Hyup Lee
Batch and continuous fixed-bed column studies were investigated using electrochemically modified biochar calcium-alginate beads (EMB-CABs) as an adsorbent for the removal of phosphate from aqueous solutions. Batch experiments revealed that the phosphate adsorption behavior of EMB-CABs and its structural characteristics were highly dependent on pH condition. Also, kinetics and equilibrium isotherms studies demonstrated that the experimental data correlated well with the pseudo-second-order and Sips isotherm models, respectively...
July 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28772896/functionalization-of-magnetic-chitosan-particles-for-the-sorption-of-u-vi-cu-ii-and-zn-ii-hydrazide-derivative-of-glycine-grafted-chitosan
#12
Mohammed F Hamza, Mohsen M Aly, Adel A-H Abdel-Rahman, Samar Ramadan, Heba Raslan, Shengye Wang, Thierry Vincent, Eric Guibal
A new magnetic functionalized derivative of chitosan is synthesized and characterized for the sorption of metal ions (environmental applications and metal valorization). The chemical modification of the glycine derivative of chitosan consists of: activation of the magnetic support with epichlorohydrin, followed by reaction with either glycine to produce the reference material (i.e., Gly sorbent) or glycine ester hydrochloride, followed by hydrazinolysis to synthesize the hydrazide functionalized sorbent (i...
May 16, 2017: Materials
https://www.readbyqxmd.com/read/28772246/early-alcohol-use-with-parental-permission-psychosocial-characteristics-and-drinking-in-late-adolescence
#13
Craig R Colder, Kathleen Shyhalla, Seth E Frndak
The earliest experiences with alcohol for many children occur in the family context with parental supervision. The current study examined individual and sociocultural characteristics associated with early (prior to age 13years) sipping and tasting alcohol with parental permission in two longitudinal community samples. Early sipping/tasting was also tested as a predictor of frequency and quantity of alcohol use, and alcohol-related problems seven years later in late adolescence. Early sipping/tasting with parental permission was associated with a sociocultural context supportive of alcohol use (e...
July 25, 2017: Addictive Behaviors
https://www.readbyqxmd.com/read/28770308/hemoglobin-level-at-stage-1-discharge-has-no-impact-on-inter-stage-growth-and-stability-in-single-ventricle-infants
#14
Claudia Delgado-Corcoran, Deborah U Frank, Stephanie Bodily, Chong Zhang, Katherine H Wolpert, Kathryn Lucas, Theodore J Pysher, Angela P Presson, Susan L Bratton
Hemoglobin levels (Hgb) of infants with a single ventricle (SV) are traditionally maintained high to maximize oxygen-carrying capacity during stage 1 palliation (S1P), stage 2 palliation (S2P), and between stages (IS). A single-center observational cohort study was performed to determine if red blood cell transfusion during the convalescent phase of the S1P (late S1P transfusion) to achieve higher Hgb is associated with benefits during the IS including improved growth and decreased acute medical events. 137 infants <1 year with SV with SIP undergoing care from January 2008 to June 2015 were retrospectively evaluated...
August 3, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/28758749/solvent-shared-ion-pairs-at-the-air-solution-interface-of-magnesium-chloride-and-sulfate-solutions-revealed-by-sum-frequency-spectroscopy-and-molecular-dynamics-simulations
#15
Lisa Götte, Krista M Parry, Wei Hua, Dominique Verreault, Heather C Allen, Douglas J Tobias
The ion distribution and ion pairing properties of Mg(2+), SO4(2-), NO3(-), and Cl(-) in the interfacial region of MgSO4, Mg(NO3)2, and MgCl2 solutions were investigated using vibrational sum frequency generation (VSFG) spectroscopy and molecular dynamics (MD) simulations. An electric field reversal relative to Mg(NO3)2 and MgCl2 solutions is observed at the interface of a MgSO4 solution. We show that, although magnesium cations are expected to have preference for bulk solvation, solvent-shared ion pairs (SIPs) exist in the interfacial region in which Mg(2+) cations are closer to the solution surface than sulfate anions...
August 16, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28757159/effectivity-of-oral-recombinant-dna-vaccine-against-streptococcus-agalactiae-in-nile-tilapia
#16
Ling Zhu, Qian Yang, Lingyuan Huang, Kaiyu Wang, Xingli Wang, Defang Chen, Yi Geng, Xiaoli Huang, Ping Ouyang, Weimin Lai
The effects of oral vaccination by two immune routes (gavage and mixed fodder administration) using a recombinant DNA vaccine SL7207-pVAX1-sip against Streptococcus agalactiae were assessed. SL7207-pVAX1-sip significantly enhanced various innate immune responses of tilapia, such as total serum protein, superoxide dismutase activity, lysozyme activity, complement C3 concentration in serum, serum antibacterial activity, and interleukin 1β and tumor necrosis factor-α. Vaccinated fish had higher relative percent survival values (57% and 63% for gavage group and mix fodder administration, respectively) at 10 weeks after S...
July 28, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28756127/removal-of-nitrite-from-aqueous-solution-by-bacillus-amyloliquefaciens-biofilm-adsorption
#17
Cai Hui, Xiaoxiao Guo, Pengfei Sun, Rashid Azim Khan, Qichun Zhang, Yongchao Liang, Yu-Hua Zhao
A newly verified adsorbent biofilm produced by Bacillus amyloliquefaciens DT was investigated for nitrite removal from aqueous solutions. The biofilm's characteristics and adsorption mechanism were determined, with results indicating that nitrite ions were adsorbed onto the protonated amine sites of biofilm under acidic conditions. Analysis of various factors showed that higher nitrite adsorption capacities occurred at pH < 3.0 and higher temperatures as well as higher initial nitrite concentrations, with a maximum nitrite removal capacity of 116...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28755850/sips-as-a-model-to-study-age-related-changes-in-proteolysis-and-aggregate-formation
#18
REVIEW
Christiane Ott, Tobias Jung, Tilman Grune, Annika Höhn
Aging is accompanied by the accumulation of cellular damage over time in response to stress, lifestyle and environmental factors ultimately leading to age-related diseases and death. Additionally, the number of senescent cells increases with age. Senescence is most likely not a static endpoint, it represents a series of hallmarks including morphological changes, alterations in protein turnover and accumulation of protein aggregates. The importance of protein oxidation and aggregate accumulation in the progression of aging is not yet fully understood and research to what extent the accumulation of oxidized proteins has an effect on senescence and the aging process is still ongoing...
July 26, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/28749453/mindfulness-based-stress-reduction-therapy-improves-patient-and-caregiver-reported-outcomes-in-cirrhosis
#19
Jasmohan S Bajaj, Michael Ellwood, Timothy Ainger, Thomas Burroughs, Andrew Fagan, Edith A Gavis, Douglas M Heuman, Michael Fuchs, Binu John, James B Wade
OBJECTIVES: Patient-reported outcomes such as health-related quality of life (HRQOL) are impaired in cirrhosis due to under-treated mood and sleep disorders, which can adversely impact their caregivers. Mindfulness-based stress reduction (MBSR) can improve patient-reported outcomes (PRO) in non-cirrhotic patients but their impact in cirrhosis is unclear. To evaluate the effect of MBSR and supportive group therapy on mood, sleep and HRQOL in cirrhotic patients and their caregivers. METHODS: Cirrhotic outpatients with mild depression (Beck Depression Inventory (BDI)>14) on screening with an adult caregiver were enrolled...
July 27, 2017: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/28748706/the-development-of-new-composite-metrics-for-the-comprehensive-analytic-and-visual-assessment-of-hypoglycemia-using-the-hypo-triad
#20
Andreas Thomas, John Shin, Boyi Jiang, Chantal McMahon, Ralf Kolassa, Robert A Vigersky
BACKGROUND: Quantifying hypoglycemia has traditionally been limited to using the frequency of hypoglycemic events during a given time interval using data from blood glucose (BG) testing. However, continuous glucose monitoring (CGM) captures three parameters-a Hypo-Triad-unavailable with BG monitoring that can be used to better characterize hypoglycemia: area under the curve (AUC), time (duration of hypoglycemia), and frequency of daily episodes below a specified threshold. METHODS: We developed two new analytic metrics to enhance the traditional Hypo-Triad of CGM-derived data to more effectively capture the intensity of hypoglycemia (IntHypo) and overall hypoglycemic environment called the "hypoglycemia risk volume" (HypoRV)...
July 1, 2017: Journal of Diabetes Science and Technology
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