keyword
MENU ▼
Read by QxMD icon Read
search

lipo transfer

keyword
https://www.readbyqxmd.com/read/27623438/new-insights-into-nod-factor-biosynthesis-analyses-of-chitooligomers-and-lipo-chitooligomers-of-rhizobium-sp-irbg74-mutants
#1
Véréna Poinsot, Matthew B Crook, Stéphanie Erdn, Fabienne Maillet, Adeline Bascaules, Jean-Michel Ané
Soil-dwelling, nitrogen-fixing rhizobia signal their presence to legume hosts by secreting lipo-chitooligomers (LCOs) that are decorated with a variety of chemical substituents. It has long been assumed, but never empirically shown, that the LCO backbone is synthesized first by NodC, NodB, and NodA, followed by addition of one or more substituents by other Nod proteins. By analyzing a collection of in-frame deletion mutants of key nod genes in the bacterium Rhizobium sp. IRBG74 by mass spectrometry, we were able to shed light on the possible substitution order of LCO decorations, and we discovered that the prevailing view is probably erroneous...
November 3, 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/27476200/-evolution-of-root-nodule-bacteria-reconstruction-of-the-speciation-processes-resulting-from-genomic-rearrangements-in-a-symbiotic-system
#2
REVIEW
N A Provorov, E E Andronov
The processes of speciation and macroevolution of root nodule bacteria (rhizobia), based on deep rearrangements of their genomes and occurring in the N₂-fixing symbiotic system, are reconstructed. At the first stage of rhizobial evolution, transformation of free-living diazotrophs (related to Rhodopseudomonas) to symbiotic N₂-fixers (Bradyrhizobium) occurred due to the acquisition of the fix gene system, which is responsible for providing nitrogenase with electrons and reducing equivalents, as well as for oxygen-dependent regulation of nitrogenase synthesis in planta, and then of the nod genes responsible for the synthesis of the lipo- chito-oligosaccharide Nod factors, which induce root nodule development...
March 2016: Mikrobiologiia
https://www.readbyqxmd.com/read/27446492/label-free-cest-mri-detection-of-citicoline-liposome-drug-delivery-in-ischemic-stroke
#3
Huanling Liu, Anna Jablonska, Yuguo Li, Suyi Cao, Dexiang Liu, Hanwei Chen, Peter Cm Van Zijl, Jeff W M Bulte, Miroslaw Janowski, Piotr Walczak, Guanshu Liu
ABSTRACT Citicoline (CDPC) is a natural supplement with well-documented neuroprotective effects in the treatment of neurodegenerative diseases. In the present study, we sought to exploit citicoline as a theranostic agent with its inherent chemical exchange saturation transfer (CEST) MRI signal, which can be directly used as an MRI guidance in the citicoline drug delivery. Our in vitro CEST MRI results showed citicoline has two inherent CEST signals at +1 and +2 ppm, attributed to exchangeable hydroxyl and amine protons, respectively...
2016: Theranostics
https://www.readbyqxmd.com/read/26812005/combining-the-single-walled-carbon-nanotubes-with-low-voltage-electrical-stimulation-to-improve-accumulation-of-nanomedicines-in-tumor-for-effective-cancer-therapy
#4
Pei-Chi Lee, Cheng-Liang Peng, Ming-Jium Shieh
Effective delivery of biomolecules or functional nanoparticles into target sites has always been the primary objective for cancer therapy. We demonstrated that by combining single-walled carbon nanotubes (SWNTs) with low-voltage (LV) electrical stimulation, biomolecule delivery can be effectively enhanced through reversible electroporation (EP). Clear pore formation in the cell membrane is observed due to LV (50V) pulse electrical stimulation amplified by SWNTs. The cell morphology remains intact and high cell viability is retained...
March 10, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/24875759/direct-cytosolic-sirna-delivery-by-reconstituted-high-density-lipoprotein-for-target-specific-therapy-of-tumor-angiogenesis
#5
Yang Ding, Yazhe Wang, Jianping Zhou, Xiaochen Gu, Wei Wang, Congyan Liu, Xiuli Bao, Cheng Wang, Yuanru Li, Qiang Zhang
We described here the mechanisms by which small interfering RNA (siRNA) molecules incorporated in reconstituted high density lipoprotein (rHDL) were efficiently transferred into the cytoplasm of cells to perform target-specific therapy of tumor angiogenesis. Using fluorescent-tagged apolipoprotein A-I (apoA-I) and cholesterol-conjugated siRNA (Chol-siRNA), it was confirmed with FACS and confocal microscopic measurements that Chol-siRNA-loaded rHDL nanoparticles (rHDL/Chol-siRNA complexes) were successfully established and apoA-I certainly was attached to the surface of Chol-siRNA-loaded lipoplexes (Lipos/Chol-siRNA complexes)...
August 2014: Biomaterials
https://www.readbyqxmd.com/read/24667392/involvement-of-the-tnf-and-fasl-produced-by-cd11b-kupffer-cells-macrophages-in-ccl4-induced-acute-hepatic-injury
#6
Atsushi Sato, Hiroyuki Nakashima, Masahiro Nakashima, Masami Ikarashi, Kiyoshi Nishiyama, Manabu Kinoshita, Shuhji Seki
We previously reported that F4/80(+) Kupffer cells are subclassified into CD68(+) Kupffer cells with phagocytic and ROS producing capacity, and CD11b(+) Kupffer cells with cytokine-producing capacity. Carbon tetrachloride (CCl4)-induced hepatic injury is a well-known chemical-induced hepatocyte injury. In the present study, we investigated the immunological role of Kupffer cells/macrophages in CCl4-induced hepatitis in mice. The immunohistochemical analysis of the liver and the flow cytometry of the liver mononuclear cells showed that clodronate liposome (c-lipo) treatment greatly decreased the spindle-shaped F4/80(+) or CD68(+) cells, while the oval-shaped F4/80+ CD11b(+) cells increased...
2014: PloS One
https://www.readbyqxmd.com/read/24479833/miconazole-nitrate-bearing-ultraflexible-liposomes-for-the-treatment-of-fungal-infection
#7
Jayamanti Pandit, Minakshi Garg, Narendra Kumar Jain
Miconazole nitrate is a widely used antifungal agent, but its use in topical formulations is not efficacious because deep seated fungal infections are difficult to treat with conventional topical formulation. Miconazole nitrate loaded ultraflexible liposomes have been prepared and their topical performance has been compared with conventional liposomes containing miconazole nitrate. Various ultraflexible liposomal formulations were prepared and extensively characterized for vesicular shape, size, entrapment efficiency, degree of deformability and in-vitro skin permeation through rat skin...
June 2014: Journal of Liposome Research
https://www.readbyqxmd.com/read/24201350/plant-genetic-suppression-of-the-non-nodulation-phenotype-of-rhizobium-meliloti-host-range-nodh-mutants-gene-for-gene-interaction-in-the-alfalfa-rhizobium-symbiosis
#8
G Caetano-Anollés, P M Gresshoff
Rhizobium nodulation genes can produce active extracellular signals for legume nodulation. The R. meliloti host-range nodH gene has been postulated to mediate the transfer of a sulfate to a modified lipo-oligosaccharide, which in its sulfated form is a specific nodulation factor for alfalfa (Medicago sativa L.). We found that alfalfa was capable of effective nodulation with signal-defective and non-nodulating nodH mutants (Nnr) defining a novel gene-for-gene interaction that conditions nodulation. Bacteria-free nodules that formed spontaneously at about a 3-5% rate in unselected seed populations of alfalfa cv 'Vernal' in the total absence of Rhizobium (Nar) exhibited all the histological, regulatory and ontogenetic characteristics of alfalfa nodules...
August 1992: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/24142113/the-lipo-facelift-merging-the-face-lift-and-liposculpture-eight-years-experience-and-a-preliminary-observational-study
#9
N Pallua, T Wolter
BACKGROUND: Since the introduction of the classic submucosal aponeurotic system (SMAS) face-lift, the surgical approach to improve the changes of an aging face has evolved, and significant technical improvements have been made. However, several problems still have not been solved satisfactorily. These problems include facial lipodystrophy and changes in skin and skin texture. METHODS: The Lipo-Facelift procedure consists of facial liposculpturing performed simultaneously with a biplanar, bivectorial SMAS face-lift procedure...
December 2013: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/24009356/nonlegumes-respond-to-rhizobial-nod-factors-by-suppressing-the-innate-immune-response
#10
Yan Liang, Yangrong Cao, Kiwamu Tanaka, Sandra Thibivilliers, Jinrong Wan, Jeongmin Choi, Chang ho Kang, Jing Qiu, Gary Stacey
Virtually since the discovery of nitrogen-fixing Rhizobium-legume symbioses, researchers have dreamed of transferring this capability into nonlegume crop species (for example, corn). In general, nonlegumes were assumed to lack the ability to respond to the rhizobial lipo-chitin Nod factors, which are the essential signal molecules that trigger legume nodulation. However, our data indicate that Arabidopsis thaliana plants, as well as other nonlegumes, recognize the rhizobial Nod factor via a mechanism that results in strong suppression of microbe-associated molecular pattern (MAMP)-triggered immunity...
September 20, 2013: Science
https://www.readbyqxmd.com/read/23878392/mechanisms-of-stim1-activation-of-store-independent-leukotriene-c4-regulated-ca2-channels
#11
Xuexin Zhang, José C González-Cobos, Rainer Schindl, Martin Muik, Brian Ruhle, Rajender K Motiani, Jonathan M Bisaillon, Wei Zhang, Marc Fahrner, Margarida Barroso, Khalid Matrougui, Christoph Romanin, Mohamed Trebak
We recently showed, in primary vascular smooth muscle cells (VSMCs), that the platelet-derived growth factor activates canonical store-operated Ca(2+) entry and Ca(2+) release-activated Ca(2+) currents encoded by Orai1 and STIM1 genes. However, thrombin activates store-independent Ca(2+) selective channels contributed by both Orai3 and Orai1. These store-independent Orai3/Orai1 channels are gated by cytosolic leukotriene C4 (LTC4) and require STIM1 downstream LTC4 action. However, the source of LTC4 and the signaling mechanisms of STIM1 in the activation of this LTC4-regulated Ca(2+) (LRC) channel are unknown...
September 2013: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/23868864/surfactant-free-lipo-polymersomes-stabilized-by-iron-oxide-nanoparticles-polymer-interlayer-for-synergistically-targeted-and-magnetically-guided-gene-delivery
#12
Shang-Hsiu Hu, Tsung-Ying Hsieh, Chin-Sheng Chiang, Po-Jung Chen, You-Yin Chen, Tsung-Lang Chiu, San-Yuan Chen
Gene therapy holds promise to suppress carcinomas but still remains far removed from clinic because of the lack of a safe and effective delivery system. Besides enhancing transfection efficiency, the difficulty in gene therapy is how to deliver sufficient gene molecules to the site of interest. Herein, the rational design of surfactant-free lipo-polymersomes (LPPs) to overcome these problems is reported, simultaneously using a lipid-stabilized double emulsion approach. The LPPs are designed to conceal the cationic lipids and plasmid DNA inside the core along with iron oxide nanoparticles/polymer interlayer and a relatively neutral lipid shell to avoid the undesired interaction during circulation, leading to high accumulation in the tumors of mice...
February 2014: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/23629683/-breast-augmentation-with-autologous-fat-experience-of-96-procedures-with-the-beauli-technique
#13
D P Münch
Over the past 30 years, interest in the use of autologous fat for aesthetic body contouring, especially for breast augmentation has been continuously on the rise. The benefits of an autologous fat transplant include the absence of any inflammatory reaction to a foreign body, its harmonious appearance and a natural feeling. In earlier years, complications such as necrosis, infections or the formation of cysts, poor resorption rates as well as the difficulty of harvesting large amounts of fat within a reasonable amount of time provided grounds for criticism of the methodology of autologous fat transplantation...
April 2013: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/23597090/mechanism-of-polyplex-and-lipoplex-mediated-delivery-of-nucleic-acids-real-time-visualization-of-transient-membrane-destabilization-without-endosomal-lysis
#14
Zia ur Rehman, Dick Hoekstra, Inge S Zuhorn
Lipoplexes and polyplexes are widely applied as nonviral gene delivery carriers. Although their efficiencies of transfection are comparable, their mechanisms of delivery, specifically at the level of nucleic acid release from endosomes, are different. Thus, lipoplex-mediated release is proposed to rely on lipid mixing, as occurs between lipoplex and endosomal target membrane, the ensuing membrane destabilization leading to nucleic acid delivery into the cytosol. By contrast, the mechanism by which polyplexes, particularly those displaying a high proton buffering capacity, release their nucleic acid cargo from the endosome, is thought to rely on a so-called "proton sponge effect", in essence an osmotically induced rupturing of the endosomal membrane...
May 28, 2013: ACS Nano
https://www.readbyqxmd.com/read/23381904/a-serum-resistant-low-generation-polyamidoamine-with-pei-423-outer-layer-for-gene-delivery-vector
#15
Shirong Pan, Duanwen Cao, Huan Huang, Wu Yi, Linhao Qin, Min Feng
A new derivative of polyamidoamine and polyethylenimine, G2.5-PEI 423 or G1.5-PEI 423, is prepared by an amidation reaction of PAMAM G2.5 or PAMAM G1.5 using PEI 423. The polycations show a great ability to combine with pDNA to form complexes, which protect the pDNA from nuclease degradation. The polymers display stronger buffer capacity and lower cytotoxicity. The complexes have particle sizes of 120-180 nm and zeta potentials of 20-40 mV. The G2.5-PEI 423 complexes display much higher transfection efficiencies than PAMAM G5 and Lipo-2k, and the G1...
April 2013: Macromolecular Bioscience
https://www.readbyqxmd.com/read/23111699/lipids-and-hcv
#16
REVIEW
M F Bassendine, D A Sheridan, S H Bridge, D J Felmlee, R D G Neely
Chronic hepatitis C virus (HCV) infection is associated with an increase in hepatic steatosis and a decrease in serum levels of total cholesterol, low-density lipoprotein cholesterol (LDL) and apolipoprotein B (apoB), the main protein constituent of LDL and very low-density lipoprotein (VLDL). These changes are more marked in HCV genotype 3 infection, and effective treatment results in their reversal. Low lipid levels in HCV infection correlate not only with steatosis and more advanced liver fibrosis but also with non-response to interferon-based therapy...
January 2013: Seminars in Immunopathology
https://www.readbyqxmd.com/read/22979990/a-biomimetic-nanovector-mediated-targeted-cholesterol-conjugated-sirna-delivery-for-tumor-gene-therapy
#17
Yang Ding, Wei Wang, Meiqing Feng, Yu Wang, Jianping Zhou, Xuefang Ding, Xin Zhou, Congyan Liu, Ruoning Wang, Qiang Zhang
RNA interference holds tremendous potential as a therapeutic approach of malignant tumors. However, safe and efficient nanovectors are extremely lack for systemic delivery of small interfering RNA (siRNA). The study aimed to develop a biomimetic nanovector, reconstituted high density lipoprotein (rHDL), mediating targeted cholesterol-conjugated siRNA (Chol-siRNA) delivery for Pokemon gene silencing therapy. Chol-siRNA-loaded rHDL nanoparticles (rHDL/Chol-siRNA complexes) were prepared using thin-film dispersion method and their characteristics were investigated in detail...
December 2012: Biomaterials
https://www.readbyqxmd.com/read/22970170/a-novel-in-vivo-sirna-delivery-system-specifically-targeting-liver-cells-for-protection-of-cona-induced-fulminant-hepatitis
#18
Nan Jiang, Xusheng Zhang, Xiufen Zheng, Di Chen, Kingsun Siu, Hongmei Wang, Thomas E Ichim, Douglas Quan, Vivian McAlister, Guihua Chen, Wei-Ping Min
BACKGROUND: Fulminant hepatitis progresses to acute liver failure (ALF) when the extent of hepatocyte death exceeds the liver's regenerative capacity. Although small interfering RNA (siRNA) appears promising in animal models of hepatitis, the approach is limited by drawbacks associated with systemic administration of siRNA. The aim of this study is to develop a hepatocyte-specific delivery system of siRNA for treatment of fulminant hepatitis. METHODOLOGY/PRINCIPAL FINDINGS: Galactose-conjugated liposome nano-particles (Gal-LipoNP) bearing siRNA was prepared, and the particle size and zeta potential of Gal-LipoNP/siRNA complexes were measured...
2012: PloS One
https://www.readbyqxmd.com/read/22887832/a-highly-emissive-conjugated-polyelectrolyte-vector-for-gene-delivery-and-transfection
#19
Xuli Feng, Fengting Lv, Libing Liu, Qiong Yang, Shu Wang, Guillermo C Bazan
An intrinsically fluorescent cationic polyfluorene (CCP) has been designed, synthesized, characterized, and examined as a plasmid DNA (pDNA) delivery vector. This material facilitates nucleic acid binding, encapsulation and efficient cellular uptake. CCP can effectively protect pDNA against nuclease degradation, which is necessary for gene carriers. Green fluorescent protein (GFP) expression experiments reveal that CCP can achieve efficient delivery and transfection of pDNA encoding GFP gene with 92% efficiency, which surpasses that of commercial transfection agents, lipofectamine 2000 (Lipo) and polyethylenimine (PEI)...
October 23, 2012: Advanced Materials
https://www.readbyqxmd.com/read/22348109/changes-in-soybean-global-gene-expression-after-application-of-lipo-chitooligosaccharide-from-bradyrhizobium-japonicum-under-sub-optimal-temperature
#20
Nan Wang, Wajahatullah Khan, Donald L Smith
Lipo-chitooligosaccharides (LCOs), signal compounds produced by N(2)-fixing rhizobacteria after isoflavone induction, initiate nodule formation in host legumes. Given LCOs' structural similarity to pathogen-response-eliciting chitin oligomers, foliar application of LCOs was tested for ability to induce stress-related genes under optimal growth conditions. In order to study the effects of LCO foliar spray under stressed conditions, soybean (Glycine max) seedlings grown at optimal temperature were transferred to sub-optimal temperature...
2012: PloS One
keyword
keyword
45243
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"