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https://www.readbyqxmd.com/read/28316213/-expression-of-lim-and-sh3-protein-1-in-renal-clear-cell-carcinoma-and-its-effects-on-invasion-and-migration-of-renal-clear-cell-carcinoma-786-o-cells
#1
B Jin, L Gao, W Li, J C Chen, R M Wen, J Q Wang
Objective: To investigate the expression of LIM and SH3 protein 1 (LASP1) in renal cell carcinoma and its significance in the invasion and migration of renal clear cell carcinoma 786-O cell line. Methods: The expression level of LASP1 in 41 cases of renal cell carcinoma tissues and normal renal tissues was analyzed by immunohistochemistry. The relationship between the expression level of LASP1 and clinical characteristics was further analyzed. Expression of LASP1 in 10 cases of tumor tissues with or without lymph node metastasis was analyzed by Western blot...
March 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28281968/knockdown-of-collagen-triple-helix-repeat-containing-1-inhibits-the-proliferation-and-epithelial-to-mesenchymal-transition-in-renal-cell-carcinoma-cells
#2
Xue-Fei Jin, Hai Li, Shi Zong, Hong-Yan Li
Collagen triple helix repeat containing-1 (CTHRC1), a secreted glycoprotein, is frequently upregulated in human cancers. However, the functional role of CTHRC1 in renal cell carcinoma (RCC) remains unclear. Thus, the aim of this study was to explore the role of CTHRC1 in RCC. Our results demonstrated that CTHRC1 was upregulated in RCC tissues and cell lines. Knockdown of CTHRC1 significantly inhibits the proliferation in RCCs. Furthermore, knockdown of CTHRC1 significantly inhibited the epithelial-to-mesenchymal transition (EMT) process in RCCs, as well as suppressed RCC cell migration and invasion...
October 27, 2016: Oncology Research
https://www.readbyqxmd.com/read/28270206/meis1-inhibits-clear-cell-renal-cell-carcinoma-cells-proliferation-and-in-vitro-invasion-or-migration
#3
Jie Zhu, Liang Cui, Axiang Xu, Xiaotao Yin, Fanglong Li, Jiangping Gao
BACKGROUND: Myeloid ecotropic viral integration site 1 (MEIS1) protein plays a synergistic causative role in acute myeloid leukemia (AML). However, MEIS1 has also shown to be a potential tumor suppressor in some other cancers, such as non-small-cell lung cancer (NSCLC) and prostate cancer. Although multiple roles of MEIS1 in cancer development and progression have been identified, there is an urgent demand to discover more functions of this molecule for further therapeutic design. METHODS: MEIS1 was overexpressed via adenovirus vector in clear cell renal cell carcinoma (ccRCC) cells...
March 7, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28260014/baicalin-ameliorates-renal-fibrosis-via-inhibition-of-transforming-growth-factor-%C3%AE-1-production-and-downstream-signal-transduction
#4
Long Zheng, Chao Zhang, Long Li, Chao Hu, Mushuang Hu, Niyazi Sidikejiang, Xuanchuan Wang, Miao Lin, Ruiming Rong
Previous studies have demonstrated the potential antifibrotic effects of baicalin in vitro, via examination of 21 compounds isolated from plants. However, its biological activity and underlying mechanisms of action in vivo remain to be elucidated. The present study aimed to evaluate the effect of baicalin on renal fibrosis in vivo, and the potential signaling pathways involved. A unilateral ureteral obstruction (UUO)‑induced renal fibrosis model was established using Sprague‑Dawley rats. Baicalin was administrated intraperitoneally every 2 days for 10 days...
February 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28251886/highly-expressed-antisense-non-coding-rna-in-the-ink4-locus-promotes-5-growth-and-invasion-of-renal-clear-carcinoma-cells-via-the-%C3%AE-catenin-pathway
#5
Qingchun Li, Yuan Tian, Guangrui Hu, Yun Liang, Wei Bai, Hongjun Li
Long non-coding RNAs (LncRNAs) antisense non-coding RNA in the INK4 locus (ANRIL) is involved in several human cancers.However, the role of ANRIL in renal cell carcinoma (RCC) remains unclear. This study was aimed to explore whether and how ANRIL affects the RCC progression. First of all, the expression of ANRIL in clinical tumor tissues and 4 kinds of RCC cell lines were evaluated. After transfection, the cell viability, colony number, apoptosis, migration and invasion were all assessed. Then, the expression of proteins related to apoptosis, epithelial to mesenchymal transition (EMT) and β-catenin signaling pathway were all assessed...
March 2, 2017: Oncology Research
https://www.readbyqxmd.com/read/28249915/cobalt-chloride-induced-hypoxia-induces-epithelial-mesenchymal-transition-in-renal-carcinoma-cell-lines
#6
Ning Zhang, Baoan Hong, Changhua Zhou, Xin Du, Siqi Chen, Xiaohu Deng, Shayiremu Duoerkun, Qing Li, Yong Yang, Kan Gong
OBJECTIVE: To establish epithelial-mesenchymal transition (EMT) models in renal cell carcinoma (RCC) cell lines. MATERIALS AND METHODS: The RCC cell lines A498 and 786-O were used in the experiment and CoCl2 was used to simulate hypoxia. Cells were cultured with different concentrations of CoCl2. Morphology and changes in cytoactivity were observed. After CoCl2 treatment, the expression of HIF-1α and the changes of EMT-related molecules (E-cadherin, fibronectin) were detected...
January 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28159470/hedgehog-pathway-plays-a-vital-role-in-hiv-induced-epithelial-mesenchymal-transition-of-podocyte
#7
Xiqian Lan, Hongxiu Wen, Kang Cheng, Andrei Plagov, Seyedeh Shadafarin Marashi Shoshtari, Ashwani Malhotra, Pravin C Singhal
HIV-associated nephropathy (HIVAN) is characterized by heavy proteinuria, rapidly progressive renal failure, and distinct morphological features in the kidney. HIV-induced epithelial-mesenchymal transition (EMT) is critically important for the progression of kidney injury. In this study, we tested the role of hedgehog pathway in the HIV-induced EMT and fibrosis of kidney. We used the Tg26 mice, the abundantly used HIVAN mouse model, to investigate the activation of hedgehog pathway by HIV. Western blotting and real time PCR results showed that renal tissue expression of hedgehog pathway related molecules, including hedgehog homologous (Shh, Ihh, Dhh), PTCH, and Gli1, were increased in HIVAN (Tg26) mice; while immunofluorescent staining displayed localization PTCH expression in podocytes...
March 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28158902/metformin-attenuates-albumin-induced-alterations-in-renal-tubular-cells-in-vitro
#8
Soumaya Allouch, Shankar Munusamy
Proteinuria (albuminuria) plays a crucial role in the etiology of chronic kidney disease (CKD) via alteration of multiple signaling pathways and cellular process in renal cells. The objectives of this study are to investigate the effects of activation of the energy-sensing molecule AMP-activated kinase (AMPK) in renal cells using metformin on endoplasmic reticulum (ER) stress, AKT, mTOR, epithelial-to-mesenchymal transition (EMT), autophagy and apoptosis that are thought to mediate renal cell injury during proteinuria, and to dissect the AMPK- and non-AMPK mediated effects of metformin using an in vitro model of albumin-induced renal cell injury...
February 3, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28152509/microrna-210-3p-depletion-by-crispr-cas9-promoted-tumorigenesis-through-revival-of-twist1-in-renal-cell-carcinoma
#9
Hirofumi Yoshino, Masaya Yonemori, Kazutaka Miyamoto, Syuichi Tatarano, Satoshi Kofuji, Nijiro Nohata, Masayuki Nakagawa, Hideki Enokida
Previous studies showed that five miRNAs (miR-885-5p, miR-1274, miR-210-3p, miR-224 and miR-1290) were upregulated the most in clear cell renal cell carcinoma (ccRCC). Our focus was to understand from a clinical standpoint the functional consequences of upregulating miR-210-3p. Towards this, we utilized the CRISPR/Cas9 gene editing system to deplete miR-210-3p in RCC cell lines (786-o, A498 and Caki2) and characterized the outcomes. We observed that miR-210-3p depletion dramatically increased tumorigenesis, including altering the morphology of A498 and Caki2 cells in a manner characteristic of epithelial-mesenchymal transition (EMT)...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28127848/mir-30c-protects-diabetic-nephropathy-by-suppressing-epithelial-to-mesenchymal-transition-in-db-db-mice
#10
Yanru Zhao, Zhongwei Yin, Huaping Li, Jiahui Fan, Shenglan Yang, Chen Chen, Dao Wen Wang
Epithelial-to-mesenchymal transition (EMT) plays a significant role in tubulointerstitial fibrosis, which is a hallmark of diabetic nephropathy. Thus, identifying the mechanisms of EMT activation could be meaningful. In this study, loss of miR-30c accompanied with increased EMT was observed in renal tubules of db/db mice and cultured HK2 cells exposed to high glucose. To further explore the roles of miR-30c in EMT and tubulointerstitial fibrosis, recombinant adeno-associated viral vector was applied to manipulate the expression of miR-30c...
April 2017: Aging Cell
https://www.readbyqxmd.com/read/28123498/allicin-inhibits-tubular-epithelial-myofibroblast-transdifferentiation-under-high-glucose-conditions-in-vitro
#11
Hong Huang, Fenping Zheng, Xuehong Dong, Fang Wu, Tianfeng Wu, Hong Li
Previous studies have suggested that tubular epithelial-mesenchymal transition (EMT) is an important event in renal tubulointerstitial fibrosis, which is a clinical characteristic of diabetic nephropathy. The present study aimed to investigate the effect of allicin, the major biological active component of garlic, on the EMT of a human renal proximal tubular epithelial cell line (HK-2) cultured under high glucose concentrations. HK-2 cells were exposed for 48 h to 5.5 or 25 mmol/l D-glucose, 25 mmol/l D-glucose plus allicin (2...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28111379/knocking-down-tcf8-inhibits-high-glucose-and-angiotensin-ii-induced-epithelial-to-mesenchymal-transition-in-podocytes
#12
Changhuan Bai, Shumei Liang, Yunshan Wang, Bo Jiao
Epithelial to mesenchymal transition (EMT) is a physiological phenomenon in mammalian embryogenesis by which epithelial cells become mesenchymal stem cells. Studies have indicated that an inappropriate EMT plays a key role in a variety of pathogenic processes such as embryonic development and tumor metastasis. Moreover, recent studies have indicated EMT also plays an important role in renal fibrosis. In the current study, glucose and angiotensin II promoted EMT in podocytes as well as changes in the cellular morphology of podocytes...
January 22, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28106131/hif-1%C3%AE-induction-during-reperfusion-avoids-maladaptive-repair-after-renal-ischemia-reperfusion-involving-mir127-3p
#13
Elisa Conde, Sara Giménez-Moyano, Laura Martín-Gómez, Macarena Rodríguez, M Edurne Ramos, Elia Aguado-Fraile, Ignacio Blanco-Sanchez, Ana Saiz, María Laura García-Bermejo
Ischemia/reperfusion (I/R) leads to Acute Kidney Injury. HIF-1α is a key factor during organ response to I/R. We previously demonstrated that HIF-1α is induced during renal reperfusion, after ischemia. Here we investigate the role of HIF-1α and the HIF-1α dependent mechanisms in renal repair after ischemia. By interference of HIF-1α in a rat model of renal I/R, we observed loss of expression and mis-localization of e-cadherin and induction of α-SMA, MMP-13, TGFβ, and collagen I. Moreover, we demonstrate that HIF-1α inhibition promotes renal cell infiltrates by inducing IL-1β, TNF-α, MCP-1 and VCAM-1, through NFkB activity...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28092863/adipose-derived-stem-cells-ameliorate-renal-interstitial-fibrosis-through-inhibition-of-emt-and-inflammatory-response-via-tgf-%C3%AE-1-signaling-pathway
#14
Yan Song, Changliang Peng, Shasha Lv, Jing Cheng, Shanshan Liu, Qing Wen, Guangju Guan, Gang Liu
Adipose-derived stem cells (ADSCs) have been successfully used to treat acute kidney injury or acute renal failure. However, the effect of ADSCs on treating renal interstitial fibrosis remains unknown. Here, we assessed the therapeutic efficacy of ADSCs on renal interstitial fibrosis induced by unilateral ureter obstruction (UUO) and explored the potential mechanisms. After 7days of UUO, rats were injected with ADSCs (5×10(6)) or vehicle via tail vein. We found that ADSCs administration significantly ameliorated renal interstitial fibrosis, the occurrence of epithelial-mesenchymal transition (EMT) and inflammatory response...
January 13, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28088791/sinomenine-hydrochloride-inhibits-breast-cancer-metastasis-by-attenuating-inflammation-related-epithelial-mesenchymal-transition-and-cancer-stemness
#15
Xiao Li, Pingping Li, Chao Liu, Yu Ren, Xiaojiang Tang, Ke Wang, Jianjun He
Sinomenine hydrochloride (SH) has been investigated for its anti-tumor growth effect. We have previously reported that SH inhibited breast cancer cell proliferation via MAPKs signaling. However, whether SH could inhibit tumor metastasis has not been fully explored. In this study, we found that SH suppressed the metastasis potential of breast cancer cells. The wound healing and transwell assays showed that SH inhibited the migration and invasion ability of both 4T1 and MDA-MB-231 breast cancer cells. The orthotopic mouse model of 4T1 and the experimental mouse model of MDA-MB-231-luc (MDA-MB-231 cell line expressing firefly luciferase) demonstrated that SH treatment inhibited breast cancer metastasis by inhibiting epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties without obvious hepatotoxicity and renal toxicity...
January 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28042546/tempol-effect-on-epithelial-mesenchymal-transition-induced-by-hyperglycemia
#16
Mohammad Jafari, Farahnaz Dadras, Hamid Reza Ghadimipour, Mohamad Ali Seif Rabiei, Farhad Khoshjou
BACKGROUND: One of common mechanisms in pathophysiology of chronic kidney diseases is epithelial-mesenchymal transition (EMT). On the other hand oxidative stress has been known to participate in kidney damage of diabetic nephropathy (DN). OBJECTIVES: We studied if tempol, a well-known antioxidant agent, can ameliorate EMT in DN induced in male rats. MATERIALS AND METHODS: Twenty-seven male rats were equally divided in to 4 groups. Group I (control or C), group II (diabetic or D), group III (T) rats which was given tempol (100 mg/kg/day) by gavages for 28 days and group IV (D&T) was diabetic rats that also received same dose of tempol...
January 2017: Journal of Nephropathology
https://www.readbyqxmd.com/read/28030807/high-expression-of-the-long-non-coding-rna-heircc-promotes-renal-cell-carcinoma-metastasis-by-inducing-epithelial-mesenchymal-transition
#17
Jing Xiong, Ying Liu, Shengjun Luo, Li Jiang, Yang Zeng, Zhixiong Chen, Xiaobo Shi, Bufan Lv, Wei Tang
Increasing evidence indicates that long non-coding RNAs (lncRNAs) have been associated with cancer development. However, the contributions of lncRNAs to renal cell carcinoma (RCC) remain poorly characterized. Here, we identified a novel lncRNA, termed HEIRCC, which was up-regulated in RCC tissues through lncRNA microarray analysis and subsequent validation in 60 RCC clinical specimens and cell lines. The high expression of HEIRCC is associated closely with the clinical pathology features such as larger tumor size, poor differentiation, lymphatic metastasis...
January 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/27986420/fumarates-and-cancer
#18
Gwenny M Fuhler, Hester Eppinga, Maikel P Peppelenbosch
Accumulation of intermediate metabolites of the tricarboxylic acid (TCA) cycle in tumor cells can cause epithelial-to-mesenchymal transition (EMT), although the exact mechanisms remain elusive. Recent studies show that the oncometabolite fumarate, which accumulates in fumarate hydratase-deficient renal cancers, confers tumor aggressiveness by causing epigenetic changes in the antimetastatic miRNA cluster mir-200ba429. This may have important implications for the use of fumarates in the clinic.
January 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/27983909/tumor-protein-d52-tpd52-inhibits-growth-and-metastasis-in-renal-cell-carcinoma-cells-through-the-pi3k-akt-signaling-pathway
#19
Zhenhua Zhao, Hui Liu, Junqing Hou, Tieqiang Li, Xinyi Du, Xiaolei Zhao, Wenchao Xu, Weibo Xu, Junkai Chang
Tumor protein D52 (TPD52) is a member of the TPD52-like protein family and plays different roles in various types of malignancies. However, its role in renal cell carcinoma (RCC) is still unclear. In this study, we investigated role of TPD52 in RCC. The mechanism of TPD52 in RCC was also investigated. Our data demonstrated that the expression levels of TPD52 at both mRNA and protein were significantly decreased in RCC cells. Overexpression of TPD52 inhibited the proliferation, migration/invasion with decreased the epithelial-mesenchymal transition (EMT) phenotype in RCC cells, as well as attenuated the tumor growth in renal cancer xenografts...
November 8, 2016: Oncology Research
https://www.readbyqxmd.com/read/27959449/mir-204-regulates-epithelial-mesenchymal-transition-by-targeting-sp1-in-the-tubular-epithelial-cells-after-acute-kidney-injury-induced-by-ischemia-reperfusion
#20
Shun-Jie Chen, Ping Wu, Li-Jing Sun, Bo Zhou, Wei Niu, Shuang Liu, Fu-Jun Lin, Geng-Ru Jiang
Acute kidney injury (AKI) is a disease where kidney function is lost almost instantaneously; it can develop very rapidly over few hours to maximum of few days. Despite the advent of technology, the clinical management against this disease is very poor, and most of the time it is life-threatening. AKI has been actively regulated by extracellular matrix proteins (ECM), however, its underlying mechanism of regulation during AKI progression is very poorly understood. In this study, we explored the integrated network of mRNA and microRNAs (miRNAs) that maintains the progression of ECM after induction of AKI by lethal ischemia...
February 2017: Oncology Reports
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