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https://www.readbyqxmd.com/read/29217144/histatin-5-induces-the-reversal-of-pdr5p-mediated-fluconazole-resistance-in-saccharomyces%C3%A2-cerevisae
#1
J A da Rocha Curvelo, L F Reis de Sá, D C Clemente de Moraes, R M Soares, A Ferreira-Pereira
BACKGROUND: Candidiasis is a major opportunistic fungal infection in humans. The low number of antifungal drugs available to treat Candida infections and the increasing incidence of multidrug resistant (MDR) strains point to an urgent need of identifying new therapeutic options. The role of salivary components can provide insights for the development of new methodologies of control. OBJECTIVE: The aim of this study was to evaluate the ability of histatin-5, a constitutive immunological peptide present in saliva, in reversing fungal MDR phenotype, using a resistant Saccharomyces cerevisiae strain as model of study...
December 4, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29216827/comparative-genomic-analysis-of-brevibacterium-strains-insights-into-key-genetic-determinants-involved-in-adaptation-to-the-cheese-habitat
#2
Nguyen-Phuong Pham, Séverine Layec, Eric Dugat-Bony, Marie Vidal, Françoise Irlinger, Christophe Monnet
BACKGROUND: Brevibacterium strains are widely used for the manufacturing of surface-ripened cheeses, contributing to the breakdown of lipids and proteins and producing volatile sulfur compounds and red-orange pigments. The objective of the present study was to perform comparative genomic analyses in order to better understand the mechanisms involved in their ability to grow on the cheese surface and the differences between the strains. RESULTS: The genomes of 23 Brevibacterium strains, including twelve strains isolated from cheeses, were compared for their gene repertoire involved in salt tolerance, iron acquisition, bacteriocin production and the ability to use the energy compounds present in cheeses...
December 7, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29212198/targeting-oral-cancer-stemness-and-chemoresistance-by-isoliquiritigenin-mediated-grp78-regulation
#3
Fang-Wei Hu, Cheng-Chia Yu, Pei-Ling Hsieh, Yi-Wen Liao, Ming-Yi Lu, Pei-Ming Chu
Cancer stem cells (CSCs) are cells that drive tumorigenesis, contributing to metastasis and cancer recurrence as well as resistance to chemotherapy of oral squamous cell carcinomas (OSCC). Therefore, approaches to target CSCs become the subject of intense research for cancer therapy. In this study, we demonstrated that isoliquiritigenin, a chalcone-type flavonoid isolated from licorice root, exhibited more toxicity in oral cancer stem cells (OSCC-CSCs) compared to normal cells. Treatment of isoliquiritigenin not only inhibited the self-renewal ability but also reduced the expression of CSC markers, including the ALDH1 and CD44...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212189/quizartinib-ac220-reverses-abcg2-mediated-multidrug-resistance-in-vitro-and-in-vivo-studies
#4
Jun Li, Priyank Kumar, Nagaraju Anreddy, Yun-Kai Zhang, Yi-Jun Wang, Yanglu Chen, Tanaji T Talele, Kanav Gupta, Louis D Trombetta, Zhe-Sheng Chen
Previous reports have shown that some tyrosine kinase inhibitors (TKIs) could inhibit the ATP-binding cassette (ABC) transporters involved in multidrug resistance (MDR). Quizartinib (AC220), a potent class III receptor tyrosine kinase inhibitor (TKI), was synthesized to selectively inhibit FMS-like tyrosine kinase-3 (FLT3), a target in the treatment of acute myeloid leukemia (AML). Quizartinib is currently under clinical trials for FLT3 ITD and wild-type AML and is tested in combination with chemotherapy. While non-toxic to cell lines, quizartinib at 3 μM showed significant reversal effect on wild-type and mutant ABCG2 (R482T)-mediated MDR, and only a moderate reversal effect on mutant ABCG2 (R482G)-mediated MDR...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211738/metformin-inhibits-the-development-and-promotes-the-resensitization-of-treatment-resistant-breast-cancer
#5
Gerald Davies, Liubov Lobanova, Wojciech Dawicki, Gary Groot, John R Gordon, Matthew Bowen, Troy Harkness, Terra Arnason
Multiple drug resistant (MDR) malignancy remains a predictable and often terminal event in cancer therapy, and affects individuals with many cancer types, regardless of the stage at which they were originally diagnosed or the interval from last treatment. Protein biomarkers of MDR are not globally used for clinical decision-making, but include the overexpression of drug-efflux pumps (ABC transporter family) such as MDR-1 and BCRP, as well as HIF1α, a stress responsive transcription factor found elevated within many MDR tumors...
2017: PloS One
https://www.readbyqxmd.com/read/29210524/tsc1-enables-plastid-development-under-dark-conditions-contributing-to-rice-adaptation-to-transplantation-shock
#6
Xiaoliang Shi, Sunlu Chen, Yu Peng, Yufeng Wang, Jiugeng Chen, Zhanghua Hu, Baohe Wang, Aihong Li, Daiyin Chao, Yuhong Li, Sheng Teng
Since its domestication from wild rice thousands of years ago, rice has been cultivated largely through transplantation. During transplantation from the nursery to the paddy field, rice seedlings experience transplantation shock which affects their physiology and production. However, the mechanisms underlying transplantation shock and rice adaptation to this shock are largely unknown. Here, we isolated a transplant-sensitive chloroplast-deficient (tsc1) rice mutant that produces albino leaves after transplantation...
December 6, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29209085/integrated-genomic-and-proteomic-analyses-of-high-level-chloramphenicol-resistance-in-campylobacter-jejuni
#7
Hui Li, Yingyu Wang, Qin Fu, Yang Wang, Xiaowei Li, Congming Wu, Zhangqi Shen, Qijing Zhang, Peibin Qin, Jianzhong Shen, Xi Xia
Campylobacter jejuni is a major zoonotic pathogen, and its resistance to antibiotics is of great concern for public health. However, few studies have investigated the global changes of the entire organism with respect to antibiotic resistance. Here, we provide mechanistic insights into high-level resistance to chloramphenicol in C. jejuni, using integrated genomic and proteomic analyses. We identified 27 single nucleotide polymorphisms (SNPs) as well as an efflux pump cmeB mutation that conferred modest resistance...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208901/alternative-substrate-bound-conformation-of-bacterial-solute-binding-protein-involved-in-the-import-of-mammalian-host-glycosaminoglycans
#8
Sayoko Oiki, Reiko Kamochi, Bunzo Mikami, Kousaku Murata, Wataru Hashimoto
Glycosaminoglycans (GAGs), constituted by repeating uronate and amino sugar units, are major components of mammalian extracellular matrices. Some indigenous and pathogenic bacteria target GAGs for colonization to and/or infection of host mammalian cells. In Gram-negative pathogenic Streptobacillus moniliformis, the solute-binding protein (Smon0123)-dependent ATP-binding cassette (ABC) transporter incorporates unsaturated GAG disaccharides into the cytoplasm after depolymerization by polysaccharide lyase. Smon0123, composed of N and C domains, adopts either a substrate-free open or a substrate-bound closed form by approaching two domains at 47° in comparison with the open form...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29206199/a-novel-receptor-tyrosine-kinase-switch-promotes-gastrointestinal-stromal-tumor-drug-resistance
#9
Sergei Boichuk, Aigul Galembikova, Pavel Dunaev, Elena Valeeva, Elena Shagimardanova, Oleg Gusev, Svetlana Khaiboullina
The fact that most gastrointestinal stromal tumors (GISTs) acquire resistance to imatinib (IM)-based targeted therapy remains the main driving force to identify novel molecular targets that are capable to increase GISTs sensitivity to the current therapeutic regimens. Secondary resistance to IM in GISTs typically occurs due to several mechanisms that include hemi- or homo-zygous deletion of the wild-type KIT allele, overexpression of focal adhesion kinase (FAK) and insulin-like growth factor receptor I (IGF-1R) amplification, BRAF mutation, a RTK switch (loss of c-KIT and gain of c-MET/AXL), etc...
December 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29204984/kinesin-kif4a-is-associated-with-chemotherapeutic-drug-resistance-by-regulating-intracellular-trafficking-of-lung-resistance-related-protein
#10
Li-Na Pan, Yuan Zhang, Chang-Jun Zhu, Zhi-Xiong Dong
Multidrug resistance (MDR) is the major impediment to cancer chemotherapy. The expression of lung resistance-related protein (LRP), a non-ATP-binding cassette (ABC) transporter, is high in tumor cells, resulting in their resistance to a variety of cytotoxic drugs. However, the function of LRP in tumor drug resistance is not yet explicit. Our previous studies had shown that Kinesin KIF4A was overexpressed in cisplatin (DDP)-resistant human lung adenocarcinoma cells (A549/DDP cells) compared with A549 cells. The expression of KIF4A in A549 or A549/DDP cells significantly affects cisplatin resistance but the detailed mechanisms remain unclear...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29197975/selective-labeling-and-unlabeling-strategies-in-protein-solid-state-nmr-spectroscopy
#11
Denis Lacabanne, Beat H Meier, Anja Böckmann
Selective isotope labeling is central in NMR experiments and often allows to push the limits on the systems investigated. It has the advantage to supply additional resolution by diminishing the number of signals in the spectra. This is particularly interesting when dealing with the large protein systems which are currently becoming accessible to solid-state NMR studies. Isotope labeled proteins for NMR experiments are most often expressed in E. coli systems, where bacteria are grown in minimal media supplemented with 15NH4Cl and 13C-glucose as sole source of nitrogen and carbon...
December 2, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/29197772/exploring-stress-tolerance-mechanism-of-evolved-freshwater-strain-chlorella-sp-s30-under-30%C3%A2-g-l-salt
#12
Xuyang Li, Yizhong Yuan, Dujia Cheng, Juan Gao, Lingzhao Kong, Quanyu Zhao, Wei Wei, Yuhan Sun
Enhancement of stress tolerance to high concentration of salt and CO2 is beneficial for CO2 capture by microalgae. Adaptive evolution was performed for improving the tolerance of a freshwater strain, Chlorella sp. AE10, to 30 g/L salt. A resulting strain denoted as Chlorella sp. S30 was obtained after 46 cycles (138 days). The stress tolerance mechanism was analyzed by comparative transcriptomic analysis. Although the evolved strain could tolerate 30 g/L salt, high salinity caused loss to photosynthesis, oxidative phosphorylation, fatty acid biosynthesis and tyrosine metabolism...
November 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29196814/identification-of-candidate-pathogenicity-determinants-of-rhizoctonia-solani-ag1-ia-which-causes-sheath-blight-disease-in-rice
#13
Srayan Ghosh, Poonam Kanwar, Gopaljee Jha
Sheath blight disease is one of the predominant diseases of rice and it is caused by the necrotrophic fungal pathogen Rhizoctonia solani. The mechanistic insight about its widespread success as a broad host range pathogen is limited. In this study, we endeavor to identify pathogenicity determinants of R. solani during infection process in rice. Through RNAseq analysis, we identified a total of 65 and 232 R. solani (strain BRS1) genes to be commonly upregulated in three different rice genotypes (PB1, Tetep, and TP309) at establishment and necrotrophic phase, respectively...
December 1, 2017: Current Genetics
https://www.readbyqxmd.com/read/29194931/antibiotic-resistome-in-a-large-scale-healthy-human-gut-microbiota-deciphered-by-metagenomic-and-network-analyses
#14
Jie Feng, Bing Li, Xiaotao Jiang, Ying Yang, George F Wells, Tong Zhang, Xiaoyan Li
The human gut microbiota is an important reservoir of antibiotic resistance genes (ARGs). A metagenomic approach and network analysis were used to establish a comprehensive antibiotic resistome catalog and to obtain co-occurrence patterns between ARGs and microbial taxa in fecal samples from 180 healthy individuals from 11 different countries. In total, 507 ARG subtypes belonging to 20 ARG types were detected with abundances ranging from 7.12 × 10-7 to 2.72 × 10-1 copy of ARG/copy of 16S-rRNA gene. Tetracycline, multidrug, macrolide-lincosamide-streptogramin, bacitracin, vancomycin, beta-lactam and aminoglycoside resistance genes were the top seven most abundant ARG types...
December 1, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29194754/the-mechanism-of-nucleotide-binding-domain-dimerization-in-the-intact-maltose-transporter-as-studied-by-all-atom-molecular-dynamics-simulations
#15
Wei-Lin Hsu, Tadaomi Furuta, Minoru Sakurai
The Escherichia coli maltose transporter MalFGK2 -E belongs to the protein superfamily of ATP-binding cassette (ABC) transporters. This protein is composed of heterodimeric transmembrane domains (TMDs) MalF and MalG, and the homodimeric nucleotide-binding domains (NBDs) MalK2 . In addition to the TMDs and NBDs, the periplasmic maltose binding protein MalE captures maltose and shuttle it to the transporter. In this study, we performed all-atom molecular dynamics (MD) simulations on the maltose transporter and found that both the binding of MalE to the periplasmic side of the TMDs and binding of ATP to the MalK2 are necessary to facilitate the conformational change from the inward-facing state to the occluded state, in which MalK2 is completely dimerized...
December 1, 2017: Proteins
https://www.readbyqxmd.com/read/29192381/abcb6-an-abc-transporter-impacting-drug-response-and-disease
#16
Rebba C Boswell-Casteel, Yu Fukuda, John D Schuetz
Recent findings have discovered how insufficiency of ATP-binding cassette (ABC) transporter, ABCB6, can negatively impact human health. These advances were made possible by, first, finding that ABCB6 deficiency was the genetic basis for some severe transfusion reactions and by, second, determining that functionally impaired ABCB6 variants enhanced the severity of porphyria, i.e., diseases associated with defects in heme synthesis. ABCB6 is a broad-spectrum porphyrin transporter that is capable of both exporting and importing heme and its precursors across the plasma membrane and outer mitochondrial membrane, respectively...
November 30, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29191464/bombyx-ortholog-of-the-drosophila-eye-color-gene-brown-controls-riboflavin-transport-in-malpighian-tubules
#17
Haokun Zhang, Takashi Kiuchi, Chikara Hirayama, Susumu Katsuma, Toru Shimada
The Drosophila eye color gene brown is known to control the transport of pteridine precursors in adult eyes. The Brown protein belongs to the ATP-binding cassette (ABC) transporter G family, which includes proteins encoded by the genes brown, scarlet, and white. These genes are responsible for pigmentation in Drosophila and the domestic silkworm Bombyx mori. Although orthologs of brown are conserved among insects, the function of this gene is only known in Drosophila. Here, we elucidated the function of the B...
November 27, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29190894/microrna-212-abcg2-axis-contributes-to-development-of-imatinib-resistance-in-leukemic-cells
#18
Meike Kaehler, Johanna Ruemenapp, Daniel Gonnermann, Inga Nagel, Oliver Bruhn, Sierk Haenisch, Ole Ammerpohl, Daniela Wesch, Ingolf Cascorbi, Henrike Bruckmueller
BCR-ABL-independent resistance against tyrosine kinase inhibitor is an emerging problem in therapy of chronic myeloid leukemia. Such drug resistance can be linked to dysregulation of ATP-binding cassette (ABC)-transporters leading to increased tyrosine kinase inhibitor efflux, potentially caused by changes in microRNA expression or DNA-methylation. In an in vitro-imatinib-resistance model using K-562 cells, microRNA-212 was found to be dysregulated and inversely correlated to ABC-transporter ABCG2 expression, targeting its 3'-UTR...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29190818/role-of-cis-trans-proline-isomerization-in-the-function-of-pathogenic-enterobacterial-periplasmic-binding-proteins
#19
Paulina Cortes-Hernandez, Lenin Domínguez-Ramírez
Periplasmic Binding Proteins (PBPs) trap nutrients for their internalization into bacteria by ABC transporters. Ligand binding triggers PBP closure by bringing its two domains together like a Venus flytrap. The atomic determinants that control PBP opening and closure for nutrient capture and release are not known, although it is proposed that opening and ligand release occur while in contact with the ABC transporter for concurrent substrate translocation. In this paper we evaluated the effect of the isomerization of a conserved proline, located near the binding site, on the propensity of PBPs to open and close...
2017: PloS One
https://www.readbyqxmd.com/read/29189433/transintestinal-cholesterol-excretion-in-humans
#20
Laurens F Reeskamp, Emma C E Meessen, Albert K Groen
PURPOSE OF REVIEW: To discuss recent insights into the measurement and cellular basis of transintestinal cholesterol excretion (TICE) in humans and to explore TICE as a therapeutic target for increasing reverse cholesterol transport. RECENT FINDINGS: TICE is the net effect of cholesterol excretion by the enterocyte into the intestinal lumen and is the balance between input and output fluxes through the enterocytes. These fluxes are: cholesterol excretion into the intestinal lumen mainly via ATP-binding cassette (ABC) G5/8, cholesterol absorption from the intestine by Niemann-Pick C1 like protein 1, the uptake of plasma lipoproteins by enterocytes at the basolateral membrane, and the excretion of cholesterol in chylomicrons into the lymph...
November 16, 2017: Current Opinion in Lipidology
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