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https://www.readbyqxmd.com/read/29044224/crosstalk-between-proteins-expression-and-lysine-acetylation-in-response-to-patulin-stress-in-rhodotorula-mucilaginosa
#1
Xiangfeng Zheng, Qiya Yang, Lina Zhao, Maurice Tibiru Apaliya, Xiaoyun Zhang, Hongyin Zhang
The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein and glutathione S-transferase involved in detoxification was significantly up-regulated. In addition, the expression levels of zinc-binding oxidoreductase and quinone oxidoreductase that are involved in antioxidant process, ABC transport and MFS transport responsible for chemical transport were activated when treated with patulin...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042504/unintended-laboratory-driven-evolution-reveals-genetic-requirements-for-biofilm-formation-by-desulfovibrio-vulgaris-hildenborough
#2
Kara B De León, Grant M Zane, Valentine V Trotter, Gregory P Krantz, Adam P Arkin, Gareth P Butland, Peter J Walian, Matthew W Fields, Judy D Wall
Biofilms of sulfate-reducing bacteria (SRB) are of particular interest as members of this group are culprits in corrosion of industrial metal and concrete pipelines as well as being key players in subsurface metal cycling. Yet the mechanism of biofilm formation by these bacteria has not been determined. Here we show that two supposedly identical wild-type cultures of the SRB Desulfovibrio vulgaris Hildenborough maintained in different laboratories have diverged in biofilm formation. From genome resequencing and subsequent mutant analyses, we discovered that a single nucleotide change within DVU1017, the ABC transporter of a type I secretion system (T1SS), was sufficient to eliminate biofilm formation in D...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/29036915/stat3-but-not-hif-1%C3%AE-is-important-in-mediating-hypoxia-induced-chemoresistance-in-mda-mb-231-a-triple-negative-breast-cancer-cell-line
#3
Hoda Soleymani Abyaneh, Nidhi Gupta, Aneta Radziwon-Balicka, Paul Jurasz, John Seubert, Raymond Lai, Afsaneh Lavasanifar
Hypoxia-induced chemoresistance (HICR) is a well-recognized phenomenon, and in many experimental models, hypoxia inducible factor-1α (HIF-1α) is believed to be a key player. We aimed to better understand the mechanism underlying HICR in a triple negative breast cancer cell line, MDA-MB-231, with a focus on the role of HIF-1α. In this context, the effect of hypoxia on the sensitivity of MDA-MB-231 cells to cisplatin and their stem-like features was evaluated and the role of HIF-1α in both phenomena was assessed...
October 14, 2017: Cancers
https://www.readbyqxmd.com/read/29035832/new-insights-into-vinca-alkaloids-resistance-mechanism-and-circumvention-in-lung-cancer
#4
REVIEW
Ying Zhang, Shao-Hui Yang, Xiu-Li Guo
Nowadays, lung cancer, as a health problem in worldwide, has high mortality both in men and women. Despite advances in diagnosis and surgical techniques of lung cancer in recent decades, chemotherapy is still a fundamentally and extensively useful strategy. Vinca alkaloids are a class of important and widely used drugs in the treatment of lung cancer, targeting on the Vinca binding site at the exterior of microtubule plus ends. Either intrinsic or acquired resistance to chemotherapy of Vinca alkaloids has been a major obstacle to the treatment of lung cancer, which arose great interests in studies of understanding and overcoming resistance...
October 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29035735/transcriptome-profiling-identifies-genes-pathways-associated-with-experimental-resistance-to-paromomycin-in-leishmania-donovani
#5
Aditya Verma, Vasundhra Bhandari, Deepak Kumar Deep, Shyam Sundar, Jean Claude Dujardin, Ruchi Singh, Poonam Salotra
Widespread resistance towards antimony and reports of relapses following miltefosine treatment has severely affected the management of visceral leishmaniasis (VL) in the Indian subcontinent. Paromomycin (PMM), an aminoglycoside antibiotic, has been licensed for VL treatment in India in 2007. Although its use is still restricted in the field, unraveling the molecular mechanism of resistance towards PMM is the key to preserve the drug. In this study, PMM resistant lines were selected up to 100 μM of PMM in three distinct field isolates of Leishmania donovani at promastigote stage...
October 10, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29034843/research-progress-in-reversal-of-tumor-multi-drug-resistance-via-natural-products
#6
Qi Guo, Hongyan Cao, Xianghui Qi, Huikai Li, Peizhi Ye, Zhiguo Wang, Danqiao Wang, Hua Zhou, Jingui Xue, Mingyu Sun
Multidrug resistance (MDR) occurs when a tumor develops resistance to multiple chemotherapeutic drugs, which may include antitumor drugs with different chemical structures and mechanisms. MDR limits the treatment effects of antitumor drugs, and is the main cause of chemotherapy failure. MDR is caused by numerous factors including changes in ABC transporters, target proteins, detoxification, DNA repair, drug metabolic enzymes, and signal pathways of apoptosis. Clinical research indicates that natural products have great potential to treat tumors and reverse MDR...
October 16, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29032766/the-effects-of-drug-metabolizing-enzyme-inhibitors-on-hepatic-efflux-and-uptake-transporters
#7
Jonathan Cheong, Jason S Halladay, Jasleen K Sodhi, Emile Plise, Laurent Salphati
BACKGROUND: Non-selective chemical inhibitors of phase I and phase II enzymes are commonly used in in vitro metabolic studies to elucidate the biotransformation pathways of drugs. However, the inhibition of the inhibitors on efflux and uptake transporters is not well investigated, potentially leading to unexpected and ambiguous results in these studies. OBJECTIVE: The commonly used metabolizing enzyme inhibitors, 1-aminobenzotriazole (ABT), SKF-525A, pargyline, allopurinol, menadione, methimazole, piperine and raloxifene, were examined for their potential inhibition of the major hepatic ABC (ATP binding cassette) and SLC (solute carrier) transporters...
October 10, 2017: Drug Metabolism Letters
https://www.readbyqxmd.com/read/29030100/antimicrobial-susceptibility-profiles-of-oral-treponema-species
#8
Kazuko Okamoto-Shibayama, Jin Sekino, Kouki Yoshikawa, Atsushi Saito, Kazuyuki Ishihara
Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontitis. Despite its importance for successful antimicrobial treatment, information about susceptibility is limited for Treponema species. The aim of this study was to assess the susceptibility of two Treponema denticola strains, Treponema socranskii, and Treponema vincentii to eleven antimicrobial agents...
October 10, 2017: Anaerobe
https://www.readbyqxmd.com/read/29029546/bladder-cancer-stem-cells-clonal-origin-and-therapeutic-perspectives
#9
REVIEW
Yi Li, Kaisu Lin, Zhao Yang, Ning Han, Xiaofang Quan, Xiangyang Guo, Chong Li
In this article, we review the origin and therapeutic perspectives of bladder cancer stem cells (BCSCs), which are integral to the initiation, high recurrence and chemoresistance of bladder cancer. BCSCs are heterogenous and originate from multiple cell types, including urothelial stem cells and differentiated cell types, including basal, intermediate stratum and umbrella cells. Cell surface markers, including CD44, CD67LR, EMA, ALDH1A1 and BCMab1, are used to identify and isolate BCSCs. The Hedgehog, Notch, Wnt and JAK-STAT signaling pathways play key roles in maintaining the stemness, self-renewal and proliferative potential of BCSCs...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29028589/tanshinoneiia-enhances-the-chemosensitivity-of-breast-cancer-cells-to-doxorubicin-through-down-regulating-the-expression-of-mdr-related-abc-transporters
#10
Kun Li, Hong Lai
As the first-line drug for breast cancer chemotherapy, doxorubicin (Dox) has strong cardiotoxicity. Meanwhile, prolonged Dox treatment of patients with breast cancer may result in resistance of breast cancer cells to Dox and an increased number of Dox-resistant breast cancer stem cells (BCSCs), thereby easily leading to breast cancer relapse. TanshinoneIIA (Tan IIA) has anti-tumor activity in addition to its cardiovascular protective effect. By preparing Dox resistant human breast cancer MCF-7 cells, here, we wanted to assess a new use of Tan IIA in enhancing the chemosensitivity of breast cancer cells to Dox and investigated its possible mechanisms...
October 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29022498/atp-binding-cassette-exporters-structure-and-mechanism-with-a-focus-on-p-glycoprotein-and-mrp1
#11
Maite Rocío Arana, Guillermo Altenberg
The majority of proteins that belong to the ATP-binding cassette (ABC) superfamily are transporters that mediate the efflux of substrates from cells. These exporters include multidrug resistance proteins of the ABCB and ABCC subfamilies, such as P-glycoprotein (Pgp) and MRP1, respectively. These proteins are not only involved in the resistance of cancer to cytotoxic agents, but also in the protection from endo and xenobiotics, and the determination of drug pharmacokinetics, as well as in the pathophysiology of a variety of disorders...
October 12, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29018094/substrate-induced-conformational-changes-in-the-nucleotide-binding-domains-of-lipid-bilayer-associated-p-glycoprotein-during-atp-hydrolysis
#12
Maria E Zoghbi, Leo Mok, Douglas J Swartz, Anukriti Singh, Gregory A Fendley, Ina L Urbatsch, Guillermo A Altenberg
P-glycoprotein (Pgp) is an efflux pump important in multidrug resistance of cancer cells and in determining drug pharmacokinetics. Pgp is a prototype ATP-binding cassette (ABC) transporter with two nucleotide-binding domains (NBDs) that bind and hydrolyze ATP. Conformational changes at the NBDs (the Pgp engines) lead to changes across Pgp transmembrane domains that result in substrate translocation. According to current alternating access models (substrate binding pocket accessible only to one side of the membrane at a time) binding of ATP promotes NBD dimerization, resulting in external accessibility of the drug-binding site (outward-facing, closed NBD conformation), and ATP-hydrolysis leads to dissociation of the NBDs with the subsequent return of the accessibility of the binding site to the cytoplasmic side (inward-facing, open NBD conformation)...
October 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29017458/lifestyle-gene-gain-and-loss-and-transcriptional-remodeling-cause-divergence-in-the-transcriptomes-of-phytophthora-infestans-and-pythium-ultimum-during-potato-tuber-colonization
#13
Audrey M V Ah-Fong, Jolly Shrivastava, Howard S Judelson
BACKGROUND: How pathogen genomes evolve to support distinct lifestyles is not well-understood. The oomycete Phytophthora infestans, the potato blight agent, is a largely biotrophic pathogen that feeds from living host cells, which become necrotic only late in infection. The related oomycete Pythium ultimum grows saprophytically in soil and as a necrotroph in plants, causing massive tissue destruction. To learn what distinguishes their lifestyles, we compared their gene contents and expression patterns in media and a shared host, potato tuber...
October 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29016918/a-critical-view-on-abc-transporters-and-their-interacting-partners-in-auxin-transport
#14
Markus Geisler, Bibek Aryal, Martin di Donato, Pengchao Hao
Different subclasses of ATP-binding cassette (ABC) transporters have been implicated in the transport of native variants of the phytohormone auxin. Here, the putative, individual roles of key members belonging to the ABCB, ABCD and ABCG families, respectively, are highlighted and the knowledge of their assumed expression and transport routes is reviewed and compared with their mutant phenotypes. Protein-protein interactions between ABC transporters and regulatory components during auxin transport are summarized and their importance is critically discussed...
July 31, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29016119/9-deazapurines-as-broad-spectrum-inhibitors-of-the-abc-transport-proteins-p-glycoprotein-p-gp-abcb1-multidrug-resistance-associated-protein-1-mrp1-abcc1-and-breast-cancer-resistance-protein-bcrp-abcg2
#15
Katja Stefan, Sven Marcel Schmitt, Michael Wiese
P-glycoprotein (P-gp, ABCB1), Multidrug Resistance-associated Protein 1 (MRP1, ABCC1) and Breast Cancer Resistance Protein (BCRP, ABCG2) are the three major ABC transport proteins conferring resistance to many structurally diverse anticancer agents leading to the phenomenon called multidrug resistance (MDR). Much effort was put on the development of clinically useful compounds to reverse MDR. Broad-spectrum inhibitors of ABC transport proteins can be of great use in cancers that simultaneously co-express two or three transporters...
October 10, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28993692/dissecting-the-mycobacterial-cell-envelope-and-defining-the-composition-of-the-native-mycomembrane
#16
Laura Chiaradia, Cyril Lefebvre, Julien Parra, Julien Marcoux, Odile Burlet-Schiltz, Gilles Etienne, Maryelle Tropis, Mamadou Daffé
The mycobacterial envelope is unique, containing the so-called mycomembrane (MM) composed of very-long chain fatty acids, mycolic acids (MA). Presently, the molecular composition of the MM remains unproven, due to the diversity of methods used for determining its composition. The plasma membranes (PM) and the native MM-containing cell walls (MMCW) of two rapid-growing mycobacterial species, Mycobacterium aurum and M. smegmatis, were isolated from their cell lysates by differential ultracentrifugation. Transmission electron microscopy and biochemical analyses demonstrated that the two membranes were virtually pure...
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28986886/isolation-of-cancer-stem-cells-by-side-population-method
#17
Masayuki Shimoda, Masahide Ota, Yasunori Okada
The Hoechst side population (SP) method is a flow cytometry technique used to obtain stem cells based on the dye efflux properties of the ATP-binding cassette (ABC) transporters. The SP cells are characterized by their capability to efflux the fluorescent DNA-binding dye Hoechst 33342 through their ABC transporters and are enriched in stem cells, which are endowed with a self-renewal capacity and multilineage differentiation potential and express the stemness genes including ABC multidrug transporters. The protocols outlined in this book chapter describe the isolation method of the SP cells from human lung carcinoma cell lines by using Hoechst 33342...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28982816/bypassing-nearest-hospital-for-more-distant-neuroscience-care-in-head-injured-adults-with-suspected-traumatic-brain-injury-findings-of-the-head-injury-transportation-straight-to-neurosurgery-hits-ns-pilot-cluster-randomised-trial
#18
Fiona Elizabeth Lecky, Wanda Russell, Graham McClelland, Elspeth Pennington, Gordon Fuller, Steve Goodacre, Kyee Han, Andrew Curran, Damian Holliman, Nathan Chapman, Jennifer Freeman, Sonia Byers, Suzanne Mason, Hugh Potter, Timothy Coats, Kevin Mackway-Jones, Mary Peters, Jane Shewan
OBJECTIVE: Reconfiguration of trauma services, with direct transport of patients with traumatic brain injury (TBI) to specialist neuroscience centres (SNCs)-bypassing non-specialist acute hospitals (NSAHs), could improve outcomes. However, delays in stabilisation of airway, breathing and circulation (ABC) may worsen outcomes when compared with selective secondary transfer from nearest NSAH to SNC. We conducted a pilot cluster randomised controlled trial to determine the feasibility and plausibility of bypassing suspected patients with TBI -directly into SNCs-producing a measurable effect...
October 5, 2017: BMJ Open
https://www.readbyqxmd.com/read/28979839/the-periplasmic-binding-protein-nrtt-affects-xantham-gum-production-and-pathogenesis-in-xanthomonas-citri
#19
Aline Sampaio, Vanessa Rodrigues Pegos, Elisa Emiko Oshiro, Andrea Balan
In Xanthomonas citri, the bacterium that causes citrus canker, three ATP-binding cassette (ABC) transporters are known to be dedicated to the uptake of sulfur compounds. In this work, using functional, biophysical and structural methods, we showed that NrtT, a periplasmic component of the ABC transporter NrtCB, is an alkanesulfonate-binding protein and that the deletion of the nrtT gene affected xantham gum synthesis, adhesion and biofilm production, similarly to the phenotype obtained in the X. citri ssuA-knockout strain, in which the alkanesulfonate-binding protein SsuA is absent...
October 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28978010/the-network-of-dab2ip-mir-138-in-regulating-drug-resistance-of-renal-cell-carcinoma-associated-with-stem-like-phenotypes
#20
Eun-Jin Yun, Jiancheng Zhou, Chun-Jung Lin, Shan Xu, John Santoyo, Elizabeth Hernandez, Chih-Ho Lai, Ho Lin, Dalin He, Jer-Tsong Hsieh
Targeted therapy is a standard of care for metastatic renal cell carcinoma (RCC) but the response rate is not overwhelmed, which only prolongs a short survival of patients due to the onset of therapeutic resistance. Although the mechanisms are not fully understood, the presence of cancer initiating cells (CIC) may underlie the drug resistance. Nevertheless, identifying CIC phenotypes with its biomarkers in RCC appear to be diverse and controversial from many reports. In this study, we took a different approach to focus on the regulatory mechanism in RCC-CIC and unveil DAB2IP-mediated miR-138 expression that plays a critical role in modulating stem-like phenotypes in RCC via targeting the ABC transporter (ABCA13) as well as oncogenic histone methyltransferase EZH2 while down regulation of miR-138 gene expression in RCC is due to epigenetic gene silencing by DNA methyltransferase 1 (DNMT1)...
September 15, 2017: Oncotarget
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