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https://www.readbyqxmd.com/read/28732234/computational-modelling-of-genome-scale-metabolic-networks-and-its-application-to-cho-cell-cultures
#1
REVIEW
Živa Rejc, Lidija Magdevska, Tilen Tršelič, Timotej Osolin, Rok Vodopivec, Jakob Mraz, Eva Pavliha, Nikolaj Zimic, Tanja Cvitanović, Damjana Rozman, Miha Moškon, Miha Mraz
Genome-scale metabolic models (GEMs) have become increasingly important in recent years. Currently, GEMs are the most accurate in silico representation of the genotype-phenotype link. They allow us to study complex networks from the systems perspective. Their application may drastically reduce the amount of experimental and clinical work, improve diagnostic tools and increase our understanding of complex biological phenomena. GEMs have also demonstrated high potential for the optimisation of bio-based production of recombinant proteins...
July 8, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28731643/transcriptome-based-identification-of-the-optimal-reference-cho-genes-for-normalisation-of-qpcr-data
#2
Adam J Brown, Suzanne Gibson, Diane Hatton, David C James
Real-time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision, and reliability are critically dependent on the selection of internal control reference genes. In this study we have identified optimal reference genes that can be utilized universally for qPCR analysis of CHO cell mRNAs. Initially, transcriptomic datasets were analysed to identify eight endogenous genes that exhibited high expression stability across four distinct CHO cell lines sampled in different culture phases...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28728011/anti-inflammatory-effect-of-sugar-amino-acid-maillard-reaction-products-on-intestinal-inflammation-model-in%C3%A2-vitro-and-in%C3%A2-vivo
#3
Jun-Gu Oh, Su-Hyun Chun, Da Hyun Kim, Jin Hye Kim, Hye Soo Shin, Yong Soo Cho, Yong Ki Kim, Hee-Don Choi, Kwang-Won Lee
The Maillard reaction is a nonenzymatic reaction between an amino acid and a reducing sugar that usually occurs upon heating. This reaction occurs routinely in cooking, generates numerous products, which are collectively referred to as Maillard reaction products (MRPs) contributing to aroma and color features. Advanced glycation end-products (AGEs) transformed from MRPs are participated in many types of inflammation reaction. In this study, various sugar-amino acid MRPs were prepared from three different amino acids (lysine, arginine, and glycine) and sugars (glucose, fructose, and galactose) for 1 h with heating at 121 °C...
July 11, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28722325/polymer-mediated-flocculation-of-transient-cho-cultures-as-a-simple-high-throughput-method-to-facilitate-antibody-discovery
#4
Matthew G Schmitt, Yashas Rajendra, Maria D Hougland, Jeffrey S Boyles, Gavin C Barnard
Most biopharmaceutical drugs, especially monoclonal antibodies (mAbs), bispecific antibodies (BsAbs) and Fc-fusion proteins, are expressed using Chinese Hamster Ovary (CHO) cell lines. CHO cells typically yield high product titers and high product quality. Unfortunately, CHO cell lines also generate high molecular weight (HMW) aggregates of the desired product during cell culture along with CHO host cell protein (HCP) and CHO DNA. These immunogenic species, co-purified during Protein A purification, must be removed in a multi-step purification process...
July 19, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28720219/monitoring-leachables-from-single-use-bioreactor-bags-for-mammalian-cell-culture-by-dispersive-liquid-liquid-microextraction-followed-by-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#5
N Dorival-García, J Bones
A method for the identification of leachables in chemically defined media for CHO cell culture using dispersive liquid-liquid microextraction (DLLME) and UHPLC-MS is described. A Box-Behnken design of experiments (DoE) approach was applied to obtain the optimum extraction conditions of the target analytes. Performance of DLLME as extraction technique was studied by comparison of two commercial chemically defined media for CHO cell culture. General extraction conditions for any group of leachables, regardless of their specific chemical functionalities can be applied and similar optimum conditions were obtained with the two media...
July 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28710856/quantitative-intracellular-flux-modeling-and-applications-in-biotherapeutic-development-and-production-using-cho-cell-cultures
#6
REVIEW
Zhuangrong Huang, Dong-Yup Lee, Seongkyu Yoon
Chinese hamster ovary (CHO) cells have been widely used for producing many recombinant therapeutic proteins. Constraint-based modeling, such as flux balance analysis (FBA) and metabolic flux analysis (MFA), has been developing rapidly for the quantification of intracellular metabolic flux distribution at a systematic level. Such methods would produce detailed maps of flows through metabolic networks, which contribute significantly to better understanding of metabolism in cells. Although these approaches have been extensively established in microbial systems, their application to mammalian cells is sparse...
July 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28708871/ursodeoxycholic-acid-inhibits-the-proliferation-of-colon-cancer-cells-by-regulating-oxidative-stress-and-cancer-stem-like-cell-growth
#7
Eun-Kyung Kim, Jae Hee Cho, EuiJoo Kim, Yoon Jae Kim
INTRODUCTION: The regulation of reactive oxygen species (ROS) exists as a therapeutic target for cancer treatments. Previous studies have shown that ursodeoxycholic acid (UDCA) suppresses the proliferation of colon cancer cells. The aim of this study was to evaluate the effect of UDCA upon the proliferation of colon cancer cells as a direct result of the regulation of ROS. METHOD: Colon cancer cell lines (HT29 and HCT116) were treated with UDCA. The total number of cells and the number of dead cells were determined using cell counters...
2017: PloS One
https://www.readbyqxmd.com/read/28693308/isolation-and-characterization-of-a-new-porcine-epidemic-diarrhea-virus-variant-that-occurred-in-korea-in-2014
#8
Dong-Kun Yang, Ha-Hyun Kim, Seong-Hun Lee, Soon-Seek Yoon, Joong-Won Park, In-Soo Cho
Porcine epidemic diarrhea (PED) has caused significant economic losses in the swine industry, and another epidemic occurred recently in Korea in 2014. Isolating and culturing the PED virus (PEDV) allow investigations into its pathogenesis and development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets in Jeju-do. Viral propagation was confirmed in Vero cells according to cytopathic effects, immunofluorescence assay, reverse transcription-polymerase chain reaction and electron microscopic analyses...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28689014/poly-pathway-model-a-novel-approach-to-simulate-multiple-metabolic-states-by-reaction-network-based-model-application-to-amino-acid-depletion-in-cho-cell-culture
#9
Erika Hagrot, Hildur Æsa Oddsdóttir, Joan Gonzalez Hosta, Elling W Jacobsen, Véronique Chotteau
Mammalian cell lines are characterized by a complex and flexible metabolism. A single model that could describe the variations in metabolic behavior triggered by variations in the culture conditions would be a precious tool in bioprocess development. In this paper, we introduce an approach to generate a poly-pathway model and use it to simulate diverse metabolic states triggered in response to removal, reduction or doubling of amino acids in the culture medium of an antibody-producing CHO cell line. Macro-reactions were obtained from a metabolic network via elementary flux mode enumeration and the fluxes were modeled by kinetic equations with saturation and inhibition effects from external medium components...
July 5, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28687946/genome-based-reclassification-of-fusobacterium-nucleatum-subspecies-at-the-species-level
#10
Joong-Ki Kook, Soon-Nang Park, Yun Kyong Lim, Eugene Cho, Eojin Jo, Hansung Roh, Yeseul Shin, Jayoung Paek, Hwa-Sook Kim, Hongik Kim, Jeong Hwan Shin, Young-Hyo Chang
Fusobacterium nucleatum is classified as four subspecies, subsp. nucleatum, polymorphum, vincentii, and animalis, based on DNA-DNA hybridization (DDH) patterns, phenotypic characteristics, and/or multilocus sequence analysis (MLSA). The gold standards for classification of bacterial species are DDH and 16S ribosomal RNA gene (16S rDNA) sequence homology. The thresholds of DDH and 16S rDNA similarity for delineation of bacterial species have been suggested to be >70 and 98.65%, respectively. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) analysis based on genome sequences were recently introduced as a replacement for DDH to delineate bacterial species with ANI (95-96%) and GGD (70%) threshold values...
July 7, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28686724/dipeptidyl-peptidase-4-inhibitor-gemigliptin-protects-against-vascular-calcification-in-an-experimental-chronic-kidney-disease-and-vascular-smooth-muscle-cells
#11
Soon-Youn Choi, Hye-Myung Ryu, Eun-Joo Oh, Ji-Young Choi, Jang-Hee Cho, Chan-Duck Kim, Yong-Lim Kim, Sun-Hee Park
Although dipeptidyl peptidase-4 inhibitors, a class of antidiabetic drugs, have various pleiotropic effects, it remains undetermined whether gemigliptin has a beneficial effect on vascular calcification. Therefore, this study was performed to evaluate the effect of gemigliptin on vascular calcification in a rat model of adenine-induced chronic kidney disease and in cultured vascular smooth muscle cells. Gemigliptin attenuated calcification of abdominal aorta and expression of RUNX2 in adenine-induced chronic kidney disease rats...
2017: PloS One
https://www.readbyqxmd.com/read/28680378/effects-of-cellular-11%C3%AE-hydroxysteroid-dehydrogenase-1-on-lps-induced-inflammatory-responses-in-synovial-cell-line-sw982
#12
Young Sik Cho, Ki Nam Kim, Jung Hyun Shim
11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) catalyzes the conversion of inactive cortisone into active cortisol, which has pleiotropic roles in various biological conditions, such as immunological and metabolic homeostasis. Cortisol is mainly produced in the adrenal gland, but can be locally regenerated in the liver, fat, and muscle. Its diverse actions are primarily mediated by binding to the glucocorticoid receptor. SW982, a human synovial cell line, expresses 11β-HSD type 1, but not type 2, that catalyzes the conversion of cortisone to cortisol...
June 2017: Immune Network
https://www.readbyqxmd.com/read/28669590/tranexamic-acid-inhibits-melanogenesis-by-activating-the-autophagy-system-in-cultured-melanoma-cells
#13
Yeong Hee Cho, Jung Eun Park, Do Sung Lim, Jung Sup Lee
BACKGROUND: As interest in skin beauty increases, the development of new skin whitening agents has attracted substantial attention; however, the action mechanism of the agents developed so far remains largely unknown. Tranexamic acid (TXA) is commonly being used to reduce melanin synthesis in patients with melasma and also used as a raw material for functional whitening cosmetics, although its action mechanism is poorly understood. Autophagy has been well known to be essential for tissue homeostasis, adaptation to starvation, and removal of dysfunctional organelles or pathogens...
June 7, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28667738/developing-global-regression-models-for-metabolite-concentration-prediction-regardless-of-cell-line
#14
Silvère André, Sylvain Lagresle, Anthony Da Sliva, Pierre Heimendinger, Zahia Hannas, Éric Calvosa, Ludovic Duponchel
Following the Process Analytical Technology (PAT) of the Food and Drug Administration (FDA), drug manufacturers are encouraged to develop innovative techniques in order to monitor and understand their processes in a better way. Within this framework, it has been demonstrated that Raman spectroscopy coupled with chemometric tools allow to predict critical parameters of mammalian cell cultures in-line and in real time. However, the development of robust and predictive regression models clearly requires many batches in order to take into account inter-batch variability and enhance models accuracy...
July 1, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28662672/fibroblast-growth-factor-2-supports-osteoblastic-niche-cells-during-hematopoietic-homeostasis-recovery-after-bone-marrow-suppression
#15
Kyung-Ae Yoon, YeonSung Son, Young-Jin Choi, Joo-Hyun Kim, Je-Yoel Cho
BACKGROUND: Hematopoietic stem cell (HSC) maintenance requires a specific microenvironment. HSC niches can be activated by tissue damaging chemotherapeutic drugs and various cell signaling molecules such as SDF-1 and FGF, which might also result in bone marrow stress. Recent research has insufficiently shown that endosteal osteolineage cells and other niche constituents recover after marrow injury. METHODS: We investigated the role of FGF2 in the osteoblastic niche cells during hematopoietic homeostasis recovery after bone marrow injury...
June 29, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/28658531/assessment-of-acquired-mucociliary-clearance-defects-using-micro-optical-coherence-tomography
#16
Kiranya E Tipirneni, Jessica W Grayson, Shaoyan Zhang, Do-Yeon Cho, Daniel F Skinner, Dong-Jin Lim, Calvin Mackey, Guillermo J Tearney, Steven M Rowe, Bradford A Woodworth
BACKGROUND: Dehydration of airway surface liquid (ASL) disrupts normal mucociliary clearance (MCC) in sinonasal epithelium, which may lead to chronic rhinosinusitis (CRS). Abnormal chloride (Cl(-) ) transport is one such mechanism that contributes to this disorder and can be acquired secondary to environmental perturbations, such as hypoxia at the tissue surface. The objective of this study was to assess the technological feasibility of the novel micro-optical coherence tomography (μOCT) imaging technique for investigating acquired MCC defects in cultured human sinonasal epithelial (HSNE) cells...
June 28, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28657737/structure-pungency-relationships-and-trp-channel-activation-of-drimane-sesquiterpenes-in-tasmanian-pepper-tasmannia-lanceolata
#17
Klaus Mathie, Johanna Lainer, Stefan Spreng, Corinna Dawid, David A Andersson, Stuart Bevan, Thomas Hofmann
Sensory-guided fractionation of extracts of Tasmanian pepper berries revealed 20 drimane sesquiterpens, among which polygodial, warburganal, and 1β-acetoxy-9-deoxy-isomuzigadial exhibited the lowest pungency threshold concentrations on the tongue surface (0.6-2.8 nmol/cm(2)) and elicited a dose-dependent calcium influx into mTRPA1 expressing CHO cells with the lowest EC50 values (4.5 ± 1.0 to 16.7 ± 7.5 μmol/L) and a good correlation to oral pungency thresholds (R(2) = 0.986, linear regression). Calcium imaging assays demonstrated these chemosensates to induce a calcium influx into cultured trigeminal neurons prepared from wildtype (TRPA1(+/+)) mice, whereas no calcium influx was observed in neurons from TRPA1 knockout mice (TRPA1(-/-)), thus confirming the α,β-unsaturated 1,4-dialdehyde structure to be the required structural motif for a low oral puncency thresholds and activation of the Transient Receptor Potential Channel A1 (TRPA1)...
July 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28654772/evidence-for-the-presence-of-cell-surface-bound-and-intracellular-bactericidal-toxins-in-the-dinoflagellate-heterocapsa-circularisquama
#18
Kichul Cho, Li Wencheng, Satoshi Takeshita, Jung-Kil Seo, Young-Ho Chung, Daekyung Kim, Tatsuya Oda
Heterocapsa circularisquama, a harmful dinoflagellate, has multiple haemolytic toxins that are considered to be involved in the toxic mechanism against shellfish and certain species of zooplankton. To evaluate the further nature of the toxins of H. circularisquama, we investigated its effects on several species of bacteria. By colony formation assay, we found that H. circularisquama had antibacterial activity toward the marine bacterium Vibrio alginolyticus in a cell density-dependent manner. When the inoculated bacterial cells were co-cultured with H...
June 19, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28651148/aldo-keto-reductase-activity-after-diethylhexyl-phthalate-exposure-in-eutopic-and-ectopic-endometrial-cells
#19
La Yeon Kim, Mee Ran Kim, Jang Heub Kim, Hyun Hee Cho
OBJECTIVE: Endometriosis is a multifactorial gynaecological disease in reproductive-age women. Endometriotic tissue is characterized by high prostaglandin levels and progesterone resistance. Human aldo-keto reductases (AKRs) convert progesterone to a less potent metabolite and cause progesterone resistance. Therefore, in this study, we evaluated whether diethylhexyl phthalate (DEHP) alters AKR expression in human ectopic and eutopic endometrium. STUDY DESIGN: We used microarrays and western blotting to study the effects of DEHP, and checked the presence of AKR in endometriosis patients by enzyme-linked immunosorbent assay (ELISA)...
May 22, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28645575/prediction-of-drug-induced-immune-mediated-hepatotoxicity-using-hepatocyte-like-cells-derived-from-human-embryonic-stem-cells
#20
Dong Eon Kim, Mi-Jin Jang, Young Ran Kim, Joo-Young Lee, Eun Byul Cho, Eunha Kim, Yeji Kim, Mi Young Kim, Won-Il Jeong, Seyun Kim, Yong-Mahn Han, Seung-Hyo Lee
Drug-induced liver injury (DILI) is a leading cause of liver disease and a key safety factor during drug development. In addition to the initiation events of drug-specific hepatotoxicity, dysregulated immune responses have been proposed as major pathological events of DILI. Thus, there is a need for a reliable cell culture model with which to assess drug-induced immune reactions to predict hepatotoxicity for drug development. To this end, stem cell-derived hepatocytes have shown great potentials. Here we report that hepatocyte-like cells derived from human embryonic stem cells (hES-HLCs) can be used to evaluate drug-induced hepatotoxic immunological events...
June 20, 2017: Toxicology
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