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https://www.readbyqxmd.com/read/28228147/artificially-designed-recombinant-protein-composed-of-multiple-epitopes-of-foot-and-mouth-disease-virus-as-a-vaccine-candidate
#1
Ho-Bin Lee, Da-Chuan Piao, Jun-Yeong Lee, Jae-Yun Choi, Jin-Duck Bok, Chong-Su Cho, Sang-Kee Kang, Yun-Jaie Choi
BACKGROUND: Concerns regarding the safety of inactivated foot-and-mouth disease (FMD) vaccine have been raised since it is produced from cultured live FMD virus (FMDV). To overcome this issue, recombinant protein has been studied as an alternative vaccine. RESULTS AND CONCLUSION: We designed a chimerical multi-epitope recombinant protein (5BT), which is comprised of tandem repeats of five B cell epitopes (residue of VP1 136-162) derived from different FMDV variants and one T-cell epitope (residue of 3A 21-35)...
February 22, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28227437/effects-of-extremely-low-frequency-electromagnetic-fields-on-in-vitro-cellular-cultures-hela-and-cho
#2
A F Restrepo, V E Tobar, R J Camargo, E Franco, C R Pinedo, O Gutierrez, A F Restrepo, V E Tobar, R J Camargo, E Franco, C R Pinedo, O Gutierrez, C R Pinedo, E Franco, R J Camargo, V E Tobar, O Gutierrez, A F Restrepo
This paper presents the cellular proliferation effects of the exposure to extremely low frequency electromagnetic fields (ELF-EMF) on in-vitro cellular cultures HeLa and CHO. Through the magnetic stimulation system (MSS) the cells were exposed to magnetic fields with sinusoidal waveform at 50 Hz; initially for 40 minutes at intensities of 0.4 mT, 1.4 mT, 2.13 mT, 2.49 mT and 2.53 mT in parallel and perpendicular directions to the culture plates. Subsequently, the repetitive electromagnetic field (rEMF) was applied to 2...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224619/fl-asparaginase-mediated-downregulation-of-c-myc-promotes-1-25-oh-2-d3-induced-myeloid-differentiation-in-acute-myeloid-leukemia-cells
#3
Ju Han Song, Eunchong Park, Myun Soo Kim, Kyung-Min Cho, Su-Ho Park, Arim Lee, Jiseon Song, Hyeoung-Joon Kim, Jeong-Tae Koh, Tae Sung Kim
Treatment of acute myeloid leukemia (AML) largely depends on chemotherapy, but current regimens have been unsatisfactory for long-term remission. Although differentiation induction therapy utilizing 1,25(OH)2 D3 (VD3) has shown great promise for the improvement of AML treatment efficacy, severe side effects caused by its supraphysiological dose limit its clinical application. Here we investigated the combinatorial effect of l-asparaginase (ASNase)-mediated amino acid depletion and the latent alternation of VD3 activity on the induction of myeloid differentiation...
February 22, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28223521/nondestructive-nanostraw-intracellular-sampling-for-longitudinal-cell-monitoring
#4
Yuhong Cao, Martin Hjort, Haodong Chen, Fikri Birey, Sergio A Leal-Ortiz, Crystal M Han, Juan G Santiago, Sergiu P Paşca, Joseph C Wu, Nicholas A Melosh
Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, incorporating hollow 150-nm-diameter nanostraws (NS) within a defined sampling region. Once extracted, the cellular contents were analyzed with conventional methods, including fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28220676/cho-cells-adapted-to-inorganic-phosphate-limitation-show-higher-growth-and-higher-pyruvate-carboxylase-flux-in-phosphate-replete-conditions
#5
Vishwanathgouda Maralingannavar, Dharmeshkumar Parmar, Tejal Pant, Chetan Gadgil, Venkateswarlu Panchagnula, Mugdha Gadgil
Inorganic phosphate (Pi ) is an essential ion involved in diverse cellular processes including metabolism. Changes in cellular metabolism upon long term adaptation to Pi limitation have been reported in E. coli. Given the essential role of Pi , adaptation to Pi limitation may also result in metabolic changes in animal cells. In this study, we have adapted CHO cells producing recombinant IgG to limiting Pi conditions for 75 days. Not surprisingly, adapted cells showed better survival under Pi limitation. Here, we report the finding that such cells also showed better growth characteristics compared to control in batch culture replete with Pi (higher peak density and integral viable cell density), accompanied by a lower specific oxygen uptake rate and cytochrome oxidase activity towards the end of exponential phase...
February 21, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28216176/glutathione-reduces-cytotoxicity-of-polyethyleneimine-coated-magnetic-nanoparticles-in-cho-cells
#6
Klemen Strojan, Jasna Lojk, Vladimir B Bregar, Peter Veranič, Mojca Pavlin
Polyethyleneimine (PEI) is a polycationic compound frequently used as a transfection agent. However, cytotoxicity of PEI and PEI-coated nanoparticles (PEI NPs) is still a major obstacle in its use. In this study we report a method for reducing cytotoxicity of PEI NPs by addition of glutathione in NPs synthesis. Glutathione reduced cytotoxic effects for at least 30% and decreased observed oxidative stress response compared to standard formulation. Results showed that the effect was partially due to reduced zeta potential and partially due to protective antioxidant properties of glutathione...
February 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28208101/berberine-as-a-chemical-and-pharmacokinetic-marker-of-the-butanol-extracted-food-allergy-herbal-formula-2
#7
Nan Yang, Kamal Srivastava, Ying Song, Changda Liu, Sool Cho, Yujuan Chen, Xiu-Min Li
RATIONALE: Food Allergy Herbal Formula 2 (FAHF-2) provided protection against peanut anaphylaxis in a murine model and induced beneficial immune-modulation in humans. Butanol-refined FAHF-2, B-FAHF-2, retained safety and efficacy in the peanut allergic murine model at only 1/5 of FAHF-2 dosage. One compound, berberine, was isolated and identified in vitro as a bioactive component present in FAHF-2 and B-FAHF-2. The aim of this study was to investigate berberine as a chemical and pharmacokinetic marker of B-FAHF-2...
February 13, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28203118/human-u87-glioblastoma-cells-with-stemness-features-display-enhanced-sensitivity-to-natural-killer-cell-cytotoxicity-through-altered-expression-of-nkg2d-ligand
#8
Se-Jeong Oh, Jung-In Yang, Ok Kim, Eun-Jung Ahn, Woo Dae Kang, Jae-Hyuk Lee, Kyung-Sub Moon, Kyung-Hwa Lee, Duck Cho
BACKGROUND: Glioblastoma (GBM) is one of the most lethal tumors with a poor prognosis. Its inevitable recurrence is frequently explained by the presence of cancer stem cells. We aimed to show that human GBM cells with stemness features are more sensitive to natural killer (NK) cells than GBM cells without stemness characteristics. METHODS: Natural killer cell cytotoxicity was measured using flow cytometry in neurosphere-forming U87 GBM cells cultured with neurobasal media (NBE condition) and compared with that in serum-cultured U87 GBM cells (serum condition)...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28197999/parallel-experimental-design-and-multivariate-analysis-provides-efficient-screening-of-cell-culture-media-supplements-to-improve-biosimilar-product-quality
#9
David Brühlmann, Michael Sokolov, Alessandro Butté, Markus Sauer, Jürgen Hemberger, Jonathan Souquet, Hervé Broly, Martin Jordan
Rational and high-throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design-of-experiment (DoE) of CHO fed-batch cultures in 96-deepwell plates to modulate monoclonal antibody (mAb) glycosylation using medium supplements. To reduce risk of losing valuable information in an intricate joint screening, 17 compounds were separated into five different groups, considering their mode of biological action...
February 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28196955/identification-of-human-junctional-adhesion-molecule-1-as-a-functional-receptor-for-the-hom-1-calicivirus-on-human-cells
#10
Stanislav V Sosnovtsev, Carlos Sandoval-Jaime, Gabriel I Parra, Christine M Tin, Ronald W Jones, Jo Soden, Donna Barnes, Jim Freeth, Alvin W Smith, Kim Y Green
The Hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus San Miguel sea lion virus to a human host in a laboratory. We characterized the Hom-1 strain and investigated the mechanism by which human cells could be infected. An expression library of 3,559 human plasma membrane proteins was screened for reactivity with Hom-1 virus-like particles, and a single interacting protein, human junctional adhesion molecule 1 (hJAM1), was identified. Transient expression of hJAM1 conferred susceptibility to Hom-1 infection on nonpermissive Chinese hamster ovary (CHO) cells...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28194260/anti-bacterial-effects-of-enzymatically-isolated-sialic-acid-from-glycomacropeptide-in-a-helicobacter-pylori-infected-murine-model
#11
Hye-Ji Noh, Hong Bum Koh, Hee-Kyoung Kim, Hyang Hyun Cho, Jeongmin Lee
BACKGROUND/OBJECTIVES: Helicobacter pylori (H. pylori) colonization of the stomach mucosa and duodenum is the major cause of acute and chronic gastroduodenal pathology in humans. Efforts to find effective anti-bacterial strategies against H. pylori for the non-antibiotic control of H. pylori infection are urgently required. In this study, we used whey to prepare glycomacropeptide (GMP), from which sialic acid (G-SA) was enzymatically isolated. We investigated the anti-bacterial effects of G-SA against H...
February 2017: Nutrition Research and Practice
https://www.readbyqxmd.com/read/28188976/influence-of-hypoxia-on-the-stemness-of-umbilical-cord-matrix-derived-mesenchymal-stem-cells-cultured-on-chitosan-films
#12
Takashi Taguchi, Jane Y Cho, Jijun Hao, Yvette S Nout-Lomas, Kyung-Sun Kang, Dominique J Griffon
Chitosan is attractive as a substrate for stem cell expansion because it improves stemness through formation of spheroids. Hypoxia has also been proposed as a strategy to enhance stemness and survival of stem cells after in vivo implantation. This study was therefore designed to evaluate the influence of hypoxia on chitosan-induced behavior of stem cells. Umbilical cord matrix-derived stem cells were cultured on chitosan film or standard plate under normoxia and hypoxia, for 3 and 7 days. Based on immunophenotyping, chitosan strongly suppresses the expression of CD90 and CD105 cell surface markers, changes partially reversed by combined exposure to hypoxia...
February 11, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28188688/evaluation-of-heavy-chain-c-terminal-deletions-on-productivity-and-product-quality-of-monoclonal-antibodies-in-chinese-hamster-ovary-cho-cells
#13
Zhilan Hu, Danming Tang, Shahram Misaghi, Guoying Jiang, Christopher Yu, Mandy Yim, David Shaw, Brad Snedecor, Michael Laird, Amy Shen
Monoclonal antibodies (mAbs) have been well established as potent therapeutic agents and are used to treat many different diseases. During cell culture production, antibody charge variants can be generated by cleavage of heavy chain (HC) C-terminal lysine and proline amidation. Differences in levels of charge variants during manufacturing process changes make it challenging to demonstrate process comparability. In order to reduce heterogeneity and achieve consistent product quality, we generated and expressed antibodies with deletion of either HC C-terminal lysine (-K) or lysine and glycine (-GK)...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28187683/proteomics-profiling-of-chimeric-truncated-tissue-plasminogen-activator-producing-chinese-hamster-ovary-cells-cultivated-in-a-chemically-defined-medium-supplemented-with-protein-hydrolysates
#14
Bahareh Azarian, Seyedeh Matin Sajedin, Amin Azimi, Mozhgan Raigani, Behrouz Vaziri, Fatemeh Davami
Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) cells producing tissue plasminogen activator in a serum-free medium (SFM) supplemented with designed hydrolysate additives was compared to that of rCHO cells cultivated in SFM...
11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28186329/effects-of-antibody-disulfide-bond-reduction-on-purification-process-performance-and-final-drug-substance-stability
#15
Wai Keen Chung, Brian Russell, Yanhong Yang, Michael Handlogten, Suzanne Hudak, Mingyan Cao, Jihong Wang, David Robbins, Sanjeev Ahuja, Min Zhu
Antibody disulfide bond reduction during monoclonal antibody (mAb) production is a phenomenon that has been attributed to the reducing enzymes from CHO cells acting on the mAb during the harvest process. However, the impact of antibody reduction on the downstream purification process has not been studied. During the production of an IgG2 mAb, antibody reduction was observed in the harvested cell culture fluid (HCCF), resulting in high fragment levels. In addition, aggregate levels increased during the low pH treatment step in the purification process...
February 10, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28186143/the-first-complete-genome-sequences-of-the-aci-lineage-the-most-abundant-freshwater-actinobacteria-obtained-by-whole-genome-amplification-of-dilution-to-extinction-cultures
#16
Ilnam Kang, Suhyun Kim, Md Rashedul Islam, Jang-Cheon Cho
The acI lineage of the phylum Actinobacteria is the most abundant bacterial group in most freshwater lakes. However, due to difficulties in laboratory cultivation, only two mixed cultures and some incomplete single-amplified or metagenome-derived genomes have been reported for the lineage. Here, we report the initial cultivation and complete genome sequences of four novel strains of the acI lineage from the tribes acI-A1, -A4, -A7, and -C1. The acI strains, initially isolated by dilution-to-extinction culturing, eventually failed to be maintained as axenic cultures...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28183666/fabrication-of-electrospun-hpgl-scaffolds-via-glycidyl-methacrylate-cross-linker-morphology-mechanical-and-biological-properties
#17
Fernando José Costa Baratéla, Olga Zazuco Higa, Esdras Duarte Dos Passos, Alvaro Antonio Alencar de Queiroz
Electrospinning is a suitable method to produce scaffolds composed of nanoscale to microscale fibers, which are comparable to the extracellular matrix (ECM). Hyperbranched polyglycerol (HPGL) is a highly biocompatible polyether polyol potentially useful for the design of fibrous scaffolds mimicking the ECM architecture. However, scaffolds developed from HPGL have poor mechanical properties and morphological stability in the aqueous environments required for tissue engineering applications. This work reports the production of stable electrospun HPGL scaffolds (EHPGLS) using glycidyl methacrylate (GMA) as cross-linker to enhance the water stability and mechanical property of electrospun HPGL...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28183058/in%C3%A2-vitro-and-in%C3%A2-vivo-investigation-of-the-genotoxic-potential-of-waters-from-rivers-under-the-influence-of-a-petroleum-refinery-s%C3%A3-o-paulo-state-brazil
#18
Raquel Vaz Hara, Maria Aparecida Marin-Morales
In recent years concern about the chemical composition of wastewater generated by the oil refining industry has increased, even after its treatment. These wastewaters contain substances that can harm both the entire aquatic ecosystem and the health of any exposed organisms. The aim of this study was to evaluate the genotoxic and mutagenic potentials of the effluent generated by the largest Brazilian petroleum refinery, the effectiveness of the treatments used by the refinery, and whether its effluent can compromise the water quality of the river where it is discarded...
January 31, 2017: Chemosphere
https://www.readbyqxmd.com/read/28180980/myofibroblast-in-the-ligamentum-flavum-hypertrophic-activity
#19
Junseok W Hur, Taegeun Bae, Sunghyeok Ye, Joo-Hyun Kim, Sunhye Lee, Kyoungmi Kim, Seung-Hwan Lee, Jin-Soo Kim, Jang-Bo Lee, Tai-Hyoung Cho, Jung-Yul Park, Junho K Hur
PURPOSE: Majority of the previous studies compared lumbar spinal stenosis (LSS) and lumbar disc herniation (LDH) patients for analyses of LFH. However, the separation of normal/hypertrophied LF has often been ambiguous and the severity of hypertrophic activity differed. Here, we present a novel analysis scheme for LFH in which myofibroblast is proposed as a major etiological factor for LFH study. METHODS: Seventy-one LF patient tissue samples were used for this study...
February 8, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28178667/anti-gd2-ch14-18-cho-coated-nanoparticles-mediate-glioblastoma-gbm-specific-delivery-of-the-aromatase-inhibitor-letrozole-reducing-proliferation-migration-and-chemoresistance-in-patient-derived-gbm-tumor-cells
#20
Amanda Tivnan, Tatjana Heilinger, Joanne Ramsey, Gemma O'Connor, Jenny Pokorny, Jann Sarkaria, Brett Stringer, Bryan Day, Andrew Boyd, Ella Kim, Holger Lode, Sally-Ann Cryan, Jochen Prehn
Aromatase is a critical enzyme in the irreversible conversion of androgens to oestrogens, with inhibition used clinically in hormone-dependent malignancies. We tested the hypothesis that targeted aromatase inhibition in an aggressive brain cancer called glioblastoma (GBM) may represent a new treatment strategy. In this study, aromatase inhibition was achieved using third generation inhibitor, Letrozole, encapsulated within the core of biodegradable poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs). PLGA-NPs were conjugated to human/mouse chimeric anti-GD2 antibody ch14...
February 3, 2017: Oncotarget
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