keyword
MENU ▼
Read by QxMD icon Read
search

Antibody purification

keyword
https://www.readbyqxmd.com/read/29669811/linker-engineering-in-anti-tag-72-antibody-fragments-optimizes-biophysical-properties-serum-half-life-and-high-specificity-tumor-imaging
#1
Nicholas E Long, Brandon J Sullivan, Haiming Ding, Stephanie R Doll, Michael A Ryan, Charles L Hitchcock, Edward W Martin, Krishan Kumar, Michael F Tweedle, Thomas J Magliery
Antibody (Ab) fragments have great clinical potential as cancer therapeutics and diagnostics. Their small size allows for fast clearance from blood, low immunoreactivity, better tumor penetration, and simpler engineering and production. The smallest fragment derived from a full-length IgG that retains binding to its antigen, the single-chain variable fragment (scFV), is engineered by fusing the variable light and variable heavy domains with a peptide linker. Along with switching the domain orientation, altering the length and amino acid sequence of the linker can significantly affect scFV binding, stability, quaternary structure, and other biophysical properties...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669786/rearrangement-of-the-protein-phosphatase-1-interactome-during-heart-failure-progression
#2
David Y Chiang, Katherina M Alsina, Eleonora Corradini, Martin Fitzpatrick, Li Ni, Satadru K Lahiri, Julia Reynolds, Xiaolu Pan, Larry Scott, Albert J R Heck, Xander H Wehrens
Background -Heart failure (HF) is a complex disease with a rising prevalence despite advances in treatment. Protein phosphatase 1 (PP1) has long been implicated in HF pathogenesis but its exact role is both unclear and controversial. Most previous studies measured only the PP1 catalytic subunit (PP1c) without investigating its diverse set of interactors, which confer localization and substrate specificity to the holoenzyme. In this study we define the PP1 interactome in cardiac tissue and test the hypothesis that this interactome becomes rearranged during HF progression at the level of specific PP1c interactors...
April 18, 2018: Circulation
https://www.readbyqxmd.com/read/29663323/single-pass-diafiltration-integrated-into-a-fully-continuous-mab-purification-process
#3
Joanna Rucker-Pezzini, Lindsay Arnold, Kevin Hill-Byrne, Tom Sharp, Maksim Avazhanskiy, Chris Forespring
The concept of continuous manufacturing has gained significant interest from the biopharmaceutical industry over the past several years. Benefits include increased manufacturing productivity, improved quality control, reduction in plant footprint, and more flexible management of facility capacity. There are several technologies currently available that enable continuous processing for chromatography and ultrafiltration. However, a single pass diafiltration design that meets the required small molecule clearance and has been integrated into a fully continuous monoclonal antibody purification process has not been previously published...
April 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29656413/influence-of-thermal-treatment-on-the-characteristics-of-major-oyster-allergen-cra-g-1-tropomyosin
#4
Lei Fang, Guoming Li, Ruizeng Gu, Muyi Cai, Jun Lu
BACKGROUND: Shellfish, including oysters, often cause allergic reactions in adults. Thermal treatment is one of the most common technologies to deal with seafood, which may affect biological properties. The aim of this work was to evaluate the impact of heating on conformation and potential allergenicity of oyster-derived tropomyosin (Cra g 1). RESULTS: SDS-PAGE showed that there was an apparent band at 35KD of raw tropomyosin after purification and more significant polymers appeared in heated protein...
April 15, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29644623/single-pass-diafiltration-integrated-into-a-fully-continuous-mab-purification-process
#5
Joanna Rucker-Pezzini, Lindsay Arnold, Kevin Hill-Byrne, Tom Sharp, Maksim Avazhanskiy, Chris Forespring
The concept of continuous manufacturing has gained significant interest from the biopharmaceutical industry over the past several years. Benefits include increased manufacturing productivity, improved quality control, reduction in plant footprint, and more flexible management of facility capacity. There are several technologies currently available that enable continuous processing for chromatography and ultrafiltration. However, a single pass diafiltration design that meets the required small molecule clearance and has been integrated into a fully continuous monoclonal antibody purification process has not been previously published...
April 12, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29626520/efficient-protease-based-purification-of-recombinant-matrix-metalloprotease-1-in-e-coli
#6
Lokender Kumar, Warren Colomb, John Czerski, Christopher R Cox, Susanta K Sarkar
MMP1 is an essential enzyme for tissue remodeling both in normal and pathological states. We report a method of purifying activated human MMP1 in E. coli without using urea or 4-Aminophenylmercuric acetate (APMA). Instead, a non-ionic detergent, Triton X-100, was used in the lysis buffer to solubilize MMP1 followed by the protease activities of both trypsin and MMP1 to digest E. coli proteins and activate pro-MMP1. Identity of activated MMP1 was confirmed by Western blot using anti-human MMP1 antibodies, whereas the mass was determined to be 43 kD using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF-MS)...
April 4, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29626483/slyd-deficient-escherichia-coli-strains-a-highway-to-contaminant-free-protein-extraction
#7
Vladislav V Mokhonov, Ekaterina A Vasilenko, Ekaterina N Gorshkova, Irina V Astrakhantseva, Dmitry V Novikov, Viktor V Novikov
Binding of native bacterial protein SlyD to metal affinity matrices remains a major problem in affinity purification of His-tagged recombinant proteins from Escherichia coli cells. In this study, four novel E. coli strains that lack the expression of SlyD/SlyX, were engineered using λ-red mediated chromosomal deletion. The resultant mutant E. coli strains allow us to obtain SlyD-free proteins immediately after metal affinity chromatography, and eliminate additional purification processes. As a model protein, bispecific antibodies composed of anti-F4/80 VH H module and anti-TNF VH H module (MYSTI-2) were used...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29625770/temperature-dependence-of-antibody-adsorption-in-protein-a-affinity-chromatography
#8
Walpurga Krepper, Peter Satzer, Beate Maria Beyer, Alois Jungbauer
Staphylococcal protein A affinity chromatography is a well-established platform for purification of clinical-grade antibodies. The wild type ligand has been mutated to improve caustic stability, elution behavior, and/or to increase binding capacity. Several modified protein A ligands are nowadays commercially available, one of them being the thermosensitive chromatography medium Byzen Pro from Nomadic Bioscience Co., Ltd. According to the manufacturer, Byzen Pro has the ability to release IgG upon a change in temperature...
March 30, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29625688/development-of-a-general-method-for-quantifying-igg-based-therapeutic-monoclonal-antibodies-in-human-plasma-using-protein-g-purification-coupled-with-a-two-internal-standard-calibration-strategy-using-lc-ms-ms
#9
Huai-Hsuan Chiu, Hsiao-Wei Liao, Yu-Yun Shao, Yen-Shen Lu, Ching-Hung Lin, I-Lin Tsai, Ching-Hua Kuo
Monoclonal antibody (mAb) drugs have generated much interest in recent years for treating various diseases. Immunoglobulin G (IgG) represents a high percentage of mAb drugs that have been approved by the Food and Drug Administration (FDA). To facilitate therapeutic drug monitoring and pharmacokinetic/pharmacodynamic studies, we developed a general liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify the concentration of IgG-based mAbs in human plasma. Three IgG-based drugs (bevacizumab, nivolumab and pembrolizumab) were selected to demonstrate our method...
August 17, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29624810/immunodetection-of-the-toxic-portion-of-vip3a-reveals-differential-temporal-regulation-of-its-secretion-among-bacillus-thuringiensis-strains
#10
Ronaldo Costa Argôlo-Filho, Leandro Lopes Loguercio
AIMS: Devise a protocol for heterologous expression and purification of a partial toxic portion of the Bacillus thuringiensis (Bt) vegetative insecticidal protein Vip3A, using it as antigen for anti-Vip3A polyclonal antibody development. Evaluate the regulation of Vip3A secretion into culture supernatants of different Bt strains based on this antibody. METHODS AND RESULTS: A primer-pair was designed to amplify partially the toxic portion of the vip3A gene from the HD125 strain...
April 6, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29618668/establishment-of-a-madin-darby-bovine-kidney-cell-line-expressing-anchorless-bovine-prion-protein
#11
Hyo-Jin Kim, In-Soon Roh, Hoo-Chang Park, Su Bi Ahn, Tae-Young Suh, Kyung-Je Park, Hae-Eun Kang, Hyun-Joo Sohn
Enzyme-linked immunosorbent assay (ELISA) performed using extensively purified bacterially expressed bovine prion protein (PrP) shows decreased cross-reactivity. We generated a transduced Madin-Darby bovine kidney (MDBK) cell line continuously expressing glycosylphosphatidylinositol (GPI)-anchorless bovine PrP (designated as MDBK ∆GPI protein) by using a lentiviral expression system. The present study also described the method for purifying bovine PrP through sequential culturing without the need for complex purification protocol...
April 4, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29618643/comparison-of-uncleaved-and-mature-human-immunodeficiency-virus-hiv-1-membrane-envelope-glycoprotein-trimers
#12
Luis R Castillo-Menendez, Kristen Witt, Nicole Espy, Amy Princiotto, Navid Madani, Beatriz Pacheco, Andrés Finzi, Joseph Sodroski
The mature envelope glycoprotein (Env) spike on the surface of human immunodeficiency virus (HIV-1) infected cells and virions is derived by proteolytic cleavage of a trimeric gp160 glycoprotein precursor. In these studies, we compared the conformations of cleaved and uncleaved membrane Envs with truncated cytoplasmic tails to those of stabilized soluble gp140 SOSIP.664 Env trimers. Deletion of the gp41 cytoplasmic tail did not significantly affect the sensitivity of viruses with the HIV-1AD8 Env to inhibition by antibodies or a CD4-mimetic compound...
April 4, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29601533/aptamer-modified-magnetic-beads-in-biosensing
#13
REVIEW
Harshvardhan Modh, Thomas Scheper, Johanna-Gabriela Walter
Magnetic beads (MBs) are versatile tools for the purification, detection, and quantitative analysis of analytes from complex matrices. The superparamagnetic property of magnetic beads qualifies them for various analytical applications. To provide specificity, MBs can be decorated with ligands like aptamers, antibodies and peptides. In this context, aptamers are emerging as particular promising ligands due to a number of advantages. Most importantly, the chemical synthesis of aptamers enables straightforward and controlled chemical modification with linker molecules and dyes...
March 30, 2018: Sensors
https://www.readbyqxmd.com/read/29600461/detection-of-s-nitrosated-nuclear-proteins-in-pathogen-treated-arabidopsis-cell-cultures-using-biotin-switch-technique
#14
Azam Shekariesfahlan, Christian Lindermayr
Nitric oxide (NO) is an important signaling molecule involved in various plant physiological processes. The main effect of NO arises from its reaction with proteins. S-Nitrosation is the most studied NO-mediated protein posttranslational modification in plants. Via S-nitrosation, NO derivatives react with thiol groups (SHs) of protein cysteine residues and produce nitrosothiol groups (SNOs). From the time of discovering the biological function of NO in plants, an interesting case of study has been the detection of the endogenous S-nitrosated proteins in different plants, tissues, organelles, and various conditions...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29596322/development-of-polyclonal-antibody-against-clenbuterol-for-immunoassay-application
#15
Nurul Ain A Talib, Faridah Salam, Yusran Sulaiman
Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The activities of purified antibody were evaluated based on high antibody titer determined from enzyme-linked immunosorbent assay (ELISA)...
March 29, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29581010/molecular-cloning-expression-and-characterization-of-udp-n-acetyl-%C3%AE-d-galactosamine-polypeptide-n-acetylgalactosaminyltransferase-4-from-cryptosporidium-parvum
#16
Maria A DeCicco RePass, Najma Bhat, Jamie Heimburg Molinaro, Stephen Bunnell, Richard D Cummings, Honorine D Ward
Cryptosporidium spp. are the causative agents of diarrheal disease worldwide, but effective treatments are lacking. Cryptosporidium employs mucin-like glycoproteins with O-glycans to attach to and infect host intestinal epithelial cells. The Tn antigen (GalNAcα1-Ser/Thr) is an O-glycan essential for these processes, as Tn-specific lectins and a Tn-specific monoclonal antibody block attachment to and infection of host cells in vitro. The enzymes in Cryptosporidium catalyzing their synthesis, however, have not been studied...
March 23, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29569512/construction-of-a-recombinant-b-cell-epitope-vaccine-based-on-a-der-p-1-derived-hypoallergen-a-bioinformatics-approach
#17
Songwe Fanuel, Saeideh Tabesh, Kobra Mokhtarian, Esmaeil Saroddiny, Mohammad Reza Fazlollahi, Zahra Pourpak, Reza Falak, Gholam Ali Kardar
AIM: House dust mite (HDM) allergens are important elicitors of IgE-mediated allergies. This study was aimed at constructing and characterizing a recombinant fusion protein, DpTTDp, which was based on carrier-bound Der p 1-derived peptides for HDM allergen immunotherapy. METHODS: Using the Immune Epitope Database (IEDB), we identified from Der p 1, a 34-mer hypoallergenic peptide. Two copies of the hypoallergen were then fused to a partial fragment of a tetanus toxoid molecule's N-and C terminus and expressed in Escherichia coli...
March 23, 2018: Immunotherapy
https://www.readbyqxmd.com/read/29565899/facs-isolation-of-endothelial-cells-and-pericytes-from-mouse-brain-microregions
#18
Elizabeth E Crouch, Fiona Doetsch
The vasculature is emerging as a key contributor to brain function during neurodevelopment and in mature physiological and pathological states. The brain vasculature itself also exhibits regional heterogeneity, highlighting the need to develop approaches for purifying cells from different microregions. Previous approaches for isolation of endothelial cells and pericytes have predominantly required transgenic mice and large amounts of tissue, and have resulted in impure populations. In addition, the prospective purification of brain pericytes has been complicated by the fact that widely used pericyte markers are also expressed by other cell types in the brain...
April 2018: Nature Protocols
https://www.readbyqxmd.com/read/29561231/polyclonal-antibody-production-against-rgpc3-and-their-application-in-diagnosis-of-hepatocellular-carcinoma
#19
Shenghao Wang, Muhammad Kalim, Keying Liang, Jinbiao Zhan
Glypican-3 (GPC3) is an integral membrane proteoglycan, which contains a core protein anchored to the cytoplasmic membrane through a glycosyl phosphatidylinositol linkage. The glypican-3 can regulate the signaling pathways, thereby enhances cell division, growth, and apoptosis in certain cell types. It is almost non-existent on the surface of the human normal cell membrane and highly expresses on the membrane of hepatocellular carcinoma (HCC) cells. It has been well established that GPC3 provide a useful diagnostic marker...
March 21, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29551237/chemical-modification-of-protein-a-chromatography-ligands-with-polyethylene-glycol-ii-effects-on-resin-robustness-and-process-selectivity
#20
Justin Weinberg, Shaojie Zhang, Allison Kirkby, Enosh Shachar, Giorgio Carta, Todd Przybycien
We have proposed chemical modification of Protein A (ProA) chromatography ligands with polyethylene glycol (PEGylation) as a strategy to increase the resin selectivity and robustness by providing the ligand with a steric repulsion barrier against non-specific binding. Here, we report on robustness and selectivity benefits for Repligen CaptivA PriMAB resin with ligands modified with 5.2 kDa and 21.5 kDa PEG chains, respectively. PEGylation of ProA ligands allowed the resin to retain a higher percentage of static binding capacity relative to the unmodified resin upon digestion with chymotrypsin, a representative serine protease...
February 24, 2018: Journal of Chromatography. A
keyword
keyword
45211
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"