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Paramagnetic iron oxide cell tracking

Henrik Lauridsen, Casper Bindzus Foldager, Line Hansen, Michael Pedersen
Non-invasive methods to track the progress of stem cell therapies are important in the development of future regenerative therapies. Super-paramagnetic iron oxide particles (SPIOs) have previously been applied to track cells using magnetic resonance imaging (MRI) in vivo in non-regenerative animal models. To the best of the author's knowledge, the present study investigated for the first time, the feasibility of tracking SPIO labeled cells in an intrinsic regenerative environment, the regenerating limb of the axolotl, and investigated the homing of stem cell-like blastema cells to the regenerative zone...
April 2018: Experimental and Therapeutic Medicine
Pierre Danhier, Gladys Deumer, Nicolas Joudiou, Caroline Bouzin, Philippe Levêque, Vincent Haufroid, Bénédicte F Jordan, Olivier Feron, Pierre Sonveaux, Bernard Gallez
Magnetic resonance imaging (MRI) cell tracking of cancer cells labeled with superparamagnetic iron oxides (SPIO) allows visualizing metastatic cells in preclinical models. However, previous works showed that the signal void induced by SPIO on T2(*)-weighted images decreased over time. Here, we aim at characterizing the fate of iron oxide nanoparticles used in cell tracking studies and the role of macrophages in SPIO metabolism.In vivo MRI cell tracking of SPIO positive 4T1 breast cancer cells revealed a quick loss of T2* contrast after injection...
June 13, 2017: Oncotarget
Anna P Hnatiuk, Sang-Ging Ong, Fernanda D Olea, Paola Locatelli, Johannes Riegler, Won Hee Lee, Cheng Hao Jen, Andrea De Lorenzi, Carlos S Giménez, Rubén Laguens, Joseph C Wu, Alberto Crottogini
BACKGROUND: Bone marrow mesenchymal stromal cells (BMMSCs) are cardioprotective in acute myocardial infarction (AMI) because of release of paracrine angiogenic and prosurvival factors. Hypoxia-inducible factor 1-α (HIF1-α), rapidly degraded during normoxia, is stabilized during ischemia and upregulates various cardioprotective genes. We hypothesized that BMMSCs engineered to overexpress mutant, oxygen-resistant HIF1-α would confer greater cardioprotection than nontransfected BMMSCs in sheep with AMI...
July 6, 2016: Journal of the American Heart Association
Munitta Muthana, Aneurin J Kennerley, Russell Hughes, Ester Fagnano, Jay Richardson, Melanie Paul, Craig Murdoch, Fiona Wright, Christopher Payne, Mark F Lythgoe, Neil Farrow, Jon Dobson, Joe Conner, Jim M Wild, Claire Lewis
Cell-based therapy exploits modified human cells to treat diseases but its targeted application in specific tissues, particularly those lying deep in the body where direct injection is not possible, has been problematic. Here we use a magnetic resonance imaging (MRI) system to direct macrophages carrying an oncolytic virus, Seprehvir, into primary and metastatic tumour sites in mice. To achieve this, we magnetically label macrophages with super-paramagnetic iron oxide nanoparticles and apply pulsed magnetic field gradients in the direction of the tumour sites...
August 18, 2015: Nature Communications
Areesak Chotivichit, Monchai Ruangchainikom, Pipat Chiewvit, Adisak Wongkajornsilp, Kittipong Sujirattanawimol
INTRODUCTION: Intrathecal transplantation is a minimally invasive method for the delivery of stem cells, however, whether the cells migrate from the lumbar to the injured cervical spinal cord has not been proved in humans. We describe an attempt to track bone marrow-derived mesenchymal stem cells in a patient with a chronic cervical spinal cord injury. CASE PRESENTATION: A 33-year-old Thai man who sustained an incomplete spinal cord injury from the atlanto-axial subluxation was enrolled into a pilot study aiming to track bone marrow-derived mesenchymal stem cells, labeled with superparamagnetic iron oxide nanoparticles, from intrathecal transplantation in chronic cervical spinal cord injury...
April 9, 2015: Journal of Medical Case Reports
Pierre Danhier, Julie Magat, Philippe Levêque, Géraldine De Preter, Paolo E Porporato, Caroline Bouzin, Bénédicte F Jordan, Gladys Demeur, Vincent Haufroid, Olivier Feron, Pierre Sonveaux, Bernard Gallez
Cell tracking could be useful to elucidate fundamental processes of cancer biology such as metastasis. The aim of this study was to visualize, using MRI, and to quantify, using electron paramagnetic resonance (EPR), the entrapment of murine breast cancer cells labeled with superparamagnetic iron oxide particles (SPIOs) in the mouse brain after intracardiac injection. For this purpose, luciferase-expressing murine 4 T1-luc breast cancer cells were labeled with fluorescent Molday ION Rhodamine B SPIOs. Following intracardiac injection, SPIO-labeled 4 T1-luc cells were imaged using multiple gradient-echo sequences...
March 2015: NMR in Biomedicine
Yuki Mori, Ting Chen, Tetsuya Fujisawa, Syoji Kobashi, Kohji Ohno, Shinichi Yoshida, Yoshiyuki Tago, Yutaka Komai, Yutaka Hata, Yoshichika Yoshioka
Recent studies have demonstrated that immune cells play an important role in the pathogenesis of many neurological conditions. Immune cells constantly survey the brain microvasculature for irregularities in levels of factors that signal homeostasis. Immune responses are initiated when necessary, resulting in mobilisation of the microglial cells resident in the central nervous system (CNS) and/or of infiltrating peripheral cells. However, little is known about the kinetics of immune cells in healthy and diseased CNS, because it is difficult to perform long-term visualisation of cell motility in live tissue with minimal invasion...
2014: Scientific Reports
Pierre Danhier, Bernard Gallez
The purpose of this paper is to describe some of the areas where electron paramagnetic resonance (EPR) has provided unique information to MRI developments. The field of application mainly encompasses the EPR characterization of MRI paramagnetic contrast agents (gadolinium and manganese chelates, nitroxides) and superparamagnetic agents (iron oxide particles). The combined use of MRI and EPR has also been used to qualify or disqualify sources of contrast in MRI. Illustrative examples are presented with attempts to qualify oxygen sensitive contrast (i...
July 2015: Contrast Media & Molecular Imaging
Fahd Azzabi, Markus Rottmar, Virginija Jovaisaite, Markus Rudin, Tullio Sulser, Andreas Boss, Daniel Eberli
Cell therapies are a promising approach for the treatment of a variety of human conditions including stress urinary incontinence, but their success greatly depends on the biodistribution, migration, survival, and differentiation of the transplanted cells. Noninvasive in vivo cell tracking therefore presents an important aspect for translation of such a procedure into the clinics. Upon labeling with superparamagnetic iron oxide (SPIO) nanoparticles, cells can be tracked by magnetic resonance imaging (MRI), but possible adverse effect of the labeling have to be considered when labeling stem cells with SPIOs...
February 2015: Tissue Engineering. Part C, Methods
Luning Wang, William M Potter, Qun Zhao
Along with the development of modern imaging technologies, contrast agents play increasingly important roles in both clinical applications and scientific research. Super-paramagnetic iron oxide (SPIO) nanoparticles, a negative contrast agent, have been extensively used in magnetic resonance imaging (MRI), such as in vivo labeling and tracking of cells. However, there still remain many challenges, such as in vivo quantification of SPIO nanoparticles. In this work, an MR phase gradient-based method was proposed to quantify the SPIO nanoparticles...
January 2015: Contrast Media & Molecular Imaging
Rong Chen, Hui Yu, Yan-Li An, Zhen Yu-Jia, Gao-Jun Teng
Endothelial progenitor cells (EPCs) serve as cellular vehicles for targeting cancer cells and are a powerful tool for delivery of therapeutic genes. Cytosine deaminase (CD), a kind of frequent suicide gene which can kill carcinoma cells by converting a non-poisonous pro-drug 5-flucytosine (5-FC) into a poisonous cytotoxic 5-fluorouracil (5-FU). We combined super-paramagnetic iron oxide (SPIO) nanoparticles labeled EPCs with CD gene to treat grafted liver carcinomas and tracked them with 7.0 T Magnetic resonance imaging (MRI)...
February 2014: Journal of Biomedical Nanotechnology
Louise Hansen, Alastair B Hansen, Anders B Mathiasen, Michael Ng, Kishore Bhakoo, Annette Ekblond, Jens Kastrup, Tina Friis
INTRODUCTION: To evaluate survival and engraftment of mesenchymal stromal cells (MSCs) in vivo, it is necessary to track implanted cells non-invasively with a method, which does not influence cellular ultrastructure and functional characteristics. Iron-oxide particles have been applied for cell tracking for years, but knowledge regarding possible cytotoxic ultrastructural changes subsequent to iron-oxide particle labeling is limited. Hence, the purpose of this study was to label MSCs with dextran-coated ultrasmall super-paramagnetic iron-oxide (USPIO) particles conjugated with the transduction sequence of trans-activator of transcription (TAT) (IODEX-TAT) and evaluate the effect of labeling on ultrastructure, viability, phenotype and proliferative capacity of the cells...
August 2014: Scandinavian Journal of Clinical and Laboratory Investigation
Anna V Naumova, Niranjan Balu, Vasily L Yarnykh, Hans Reinecke, Charles E Murry, Chun Yuan
The main objective of cell therapy is the regeneration of damaged tissues. To distinguish graft from host tissue by magnetic resonance imaging (MRI), a paramagnetic label must be introduced to cells prior to transplantation. The paramagnetic label can be either exogenous iron oxide nanoparticles or a genetic overexpression of ferritin, an endogenous iron storage protein. The purpose of this work was to compare the efficacy of these 2 methods for MRI evaluation of engrafted cell survival in the infarcted mouse heart...
July 2014: Journal of Cardiovascular Pharmacology and Therapeutics
Anatoly Burlaka, Marina Selyuk, Marat Gafurov, Sergei Lukin, Viktoria Potaskalova, Evgeny Sidorik
PURPOSE: To study the effects of electromagnetic radiation (EMR) of ultra high frequency (UHF) in the doses equivalent to the maximal permitted energy load for the staffs of the radar stations on the biochemical processes that occur in the cell organelles. MATERIALS AND METHODS: Liver, cardiac and aorta tissues from the male rats exposed to non-thermal UHF EMR in pulsed and continuous modes were studied during 28 days after the irradiation by the electron paramagnetic resonance (EPR) methods including a spin trapping of superoxide radicals...
May 2014: International Journal of Radiation Biology
Pierre Danhier, Géraldine De Preter, Julie Magat, Quentin Godechal, Paolo E Porporato, Bénédicte F Jordan, Olivier Feron, Pierre Sonveaux, Bernard Gallez
MRI cell tracking is a promising technique for tracking various cell types in living animals. Usually, cells are incubated with iron oxides so that the particles are taken up before the cells are injected in vivo. In the present study, we aimed to monitor migration of luciferase-expressing mouse renal cancer cells (RENCA-luc) after intrarenal or intrasplenic injection. These cells were labelled using Molday Ion Rhodamine B (MIRB) fluorescent superparamagnetic iron oxide particles. Their fate after injection was first assessed using ex vivo X-band electron paramagnetic resonance (EPR) spectroscopy...
March 2014: Contrast Media & Molecular Imaging
Valentina Diana, Patrizia Bossolasco, Davide Moscatelli, Vincenzo Silani, Lidia Cova
Multipotent stem cells (SCs) could substitute damaged cells and also rescue degeneration through the secretion of trophic factors able to activate the endogenous SC compartment. Therefore, fetal SCs, characterized by high proliferation rate and devoid of ethical concern, appear promising candidate, particularly for the treatment of neurodegenerative diseases. Super Paramagnetic Iron Oxide nanoparticles (SPIOn), routinely used for pre-clinical cell imaging and already approved for clinical practice, allow tracking of transplanted SCs and characterization of their fate within the host tissue, when combined with Magnetic Resonance Imaging (MRI)...
2013: PloS One
Esmat Elhami, Bryson Dietz, Bo Xian, Jixian Deng, Fei Wang, Chao Chi, Andrew L Goertzen, Shadreck Mzengeza, Darren Freed, Rakesh C Arora, Ganghong Tian
BACKGROUND: Stem cell therapy has a promising potential for the curing of various degenerative diseases, including congestive heart failure (CHF). In this study, we determined the efficacy of different delivery methods for stem cell administration to the heart for the treatment of CHF. Both positron emission tomography (PET) and magnetic resonance imaging (MRI) were utilized to assess the distribution of delivered stem cells. METHODS: Adipose-derived stem cells of male rats were labeled with super-paramagnetic iron oxide (SPIO) and 18 F-fluorodeoxyglucose (FDG)...
October 28, 2013: EJNMMI Research
Kuan-Xiao Tang, Jin-Hua Yan, Yun-Feng Shen, Bao-Ying Li, Ying-Ming Chen, Da-Yue Liu, De-Dong Ma, Jie Li, Hua Liang, Jian-Ping Weng
BACKGROUND: Traditional cell-tracking methods fail to meet the needs of preclinical or clinical research. Thus, the aim of the present study was to establish a new method of double labeling bone marrow mesenchymal stem cells (BMSCs) from type 1 diabetic (T1D) minipigs with super-paramagnetic iron oxide (SPIO) and enhanced green fluorescent protein (eGFP) and tracing them using MRI in vitro. METHODS: Isolated BMSCs from T1D minipigs were labeled with eGFP and different concentrations of SPIO...
March 2014: Journal of Diabetes
Yesen Zhang, Yongkun Guo, Bingshan Wu, Hongtian Zhang, Chunying Liu, Jianhua Ma, Yiwu Dai, Ruxiang Xu, Zhijun Yang
Neural stem cells (NSCs) have great prospects in therapy for neurological disorders. However, the correlation between improved function and stem cell transplantation has not been fully elucidated. A non-invasive method for stem cell tracking is crucial for clinical studies. In the present study, NSCs were infected with lentiviral vectors, and the expression of transferrin receptor (TfR) in neural stem cells after lentivirus transfection (TfR-NSC) was confirmed by western blot analysis. TfR-NSCs were incubated with 1...
December 2013: Biotechnology Letters
Kang Huicong, Xue Zheng, Wang Furong, Tang Zhouping, Xu Feng, Hu Qi, Liu Xiaoyan, Huang Xiaojiang, Zhang Na, Xu Ke, Zeng Zheng, Zhu Suiqiang
Adenosine inhibits epileptic episodes by interacting with G-protein-coupled receptors. This study examined the mechanism by which the inhibitory effect of adenosine becomes impaired during epileptogenesis. Dynamic changes in adenosine A1 receptors (A1Rs) and A2a receptors (A2aRs) were investigated in a kindling model of epilepsy. RT-PCR, Western blotting, and immunofluorescence results indicated that expression of A1Rs was increased in the hippocampus 24 h after kindling, but progressively decreased 1 and 6 months after kindling...
December 2013: Molecular Neurobiology
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