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https://www.readbyqxmd.com/read/28925857/comparison-of-stored-red-blood-cell-washing-techniques-for-priming-extracorporeal-circuits
#1
Jun Sasaki, Christopher Tirotta, Hyunsoo Lim, Kathleen Kubes, Jane Salvaggio, Robert Hannan, Redmond Burke, Jorge Ojito
BACKGROUND: The aim of this study was to compare three different blood washing techniques and describe the differences for the composition of the washed red blood cells (RBC). METHODS: Stored RBCs less than 5 days old were washed using three different techniques. 1) Washing with normal saline with the COBE Model 2991 blood processor in the blood bank (BB-S). 2) Washing with normal saline with the Continuous AutoTransfusion System (C.A.T.S) in the operating room (OR-S)...
September 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28863600/ultrasonic-harmonic-generation-from-materials-with-up-to-cubic-nonlinearity
#2
Christopher M Kube, Andrea P Arguelles
This letter considers the combined effects of quadratic and cubic nonlinearity on plane wave propagation in generally anisotropic elastic solids. Displacement solutions are derived that represent the fundamental, second-, and third-harmonic waves. In arriving at the solutions, the quadratic and cubic nonlinearity parameters for generally anisotropic materials are defined. The effects of quadratic and cubic nonlinearity are shown to influence the amplitude and phase of the fundamental wave. In addition, the phase of the third-harmonic depends on a simple combination of the quadratic and cubic nonlinearity parameters...
August 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28769093/determination-of-critical-cooling-rates-in-metallic-glass-forming-alloy-libraries-through-laser-spike-annealing
#3
Punnathat Bordeenithikasem, Jingbei Liu, Sebastian A Kube, Yanglin Li, Tianxing Ma, B Ellen Scanley, Christine C Broadbridge, Joost J Vlassak, Jonathan P Singer, Jan Schroers
The glass forming ability (GFA) of metallic glasses (MGs) is quantified by the critical cooling rate (R C). Despite its key role in MG research, experimental challenges have limited measured R C to a minute fraction of known glass formers. We present a combinatorial approach to directly measure R C for large compositional ranges. This is realized through the use of compositionally-graded alloy libraries, which were photo-thermally heated by scanning laser spike annealing of an absorbing layer, then melted and cooled at various rates...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759759/ultrasonic-backscatter-from-elongated-grains-using-line-focused-ultrasound
#4
Christopher M Kube, Andrea P Arguelles, Joseph A Turner
Ultrasonic backscattering from polycrystalline materials with elongated grains is investigated. A normal incident line-focus transducer is employed such that refracted longitudinal and transverse waves are focused within the polycrystal and scatter at grain boundaries back to the transducer. A ray-based scattering model is developed to explain the dependence of the statistics of scattering measurements on grain elongation. The spatial variance of measured scattered signals from Al alloy (7475-T7) is compared to the model...
July 19, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28733848/femoro-acetabular-impingement-anatomic-study-of-reliability-and-accuracy-of-alpha-angle-and-offset-ratio-on-fifty-consecutive-peri-operatively-resected-femoral-heads
#5
Štěpán Magersky, Adam Král, Radovan Kubeš
PURPOSE: The purpose of this study was to determine whether X-ray attributes of the femoro-acetabular impingement (FAI) - alpha angle (AA) and offset ratio (OR) reflect real measurements on resected femoral heads. METHODS: FAI AA and OR were assessed on 50 consecutively-resected femoral heads. The parameters were measured on heads cut through the maximum range of the deformity and compared to the same parameters on standardized X-ray projections (anterior-posterior [AP] and axial views)...
July 21, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28463232/bispecific-antibody-targets-multiple-pseudomonas-aeruginosa-evasion-mechanisms-in-the-lung-vasculature
#6
Ajitha Thanabalasuriar, Bas Gj Surewaard, Michelle E Willson, Arpan S Neupane, Charles K Stover, Paul Warrener, George Wilson, Ashley E Keller, Bret R Sellman, Antonio DiGiandomenico, Paul Kubes
Pseudomonas aeruginosa is a major cause of severe infections that lead to bacteremia and high patient mortality. P. aeruginosa has evolved numerous evasion and subversion mechanisms that work in concert to overcome immune recognition and effector functions in hospitalized and immunosuppressed individuals. Here, we have used multilaser spinning-disk intravital microscopy to monitor the blood-borne stage in a murine bacteremic model of P. aeruginosa infection. P. aeruginosa adhered avidly to lung vasculature, where patrolling neutrophils and other immune cells were virtually blind to the pathogen's presence...
June 1, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28298664/the-impact-of-roller-pump-vs-centrifugal-pump-on-homologous-blood-transfusion-in-pediatric-cardiac-surgery
#7
Bharat Datt, Moui B Nguyen, Gary Plancher, Mark Ruzmetov, Michael O'Brien, Alicia Kube, Hamish M Munro, Kamal K Pourmoghadam, William M DeCampli
Centrifugal pumps are considered to be less destructive to blood elements (1) when compared to roller pumps. However, their large prime volumes render them unsuitable as arterial pumps in heart lung machine (HLM) circuitry for children. In November of 2014, the circuit at Arnold Palmer Hospital, a Biomedicus BP-50 with kinetic assist venous drainage (KAVD) and 1/4″ tubing was converted to a roller pump in the arterial position with gravity drainage. Vacuum-assisted venous drainage (VAVD) was mounted on the HLM as a backup, but not used...
March 2017: Journal of Extra-corporeal Technology
https://www.readbyqxmd.com/read/28253946/-periprosthetic-infection-of-the-knee-megaprosthesis-following-a-resection-of-malignant-tumours-around-the-knee
#8
J Včelák, Z Matějovský, I Kofránek, R Kubeš, J Lesenský
PURPOSE OF THE STUDY The study presents the monocentric retrospective study of a group of patients with malignant tumours around the knee, treated by a wide resection and a reconstruction with megaprosthesis due to infectious complications. Provided is a detailed analysis of each operative treatment due to the manifestation and process of periprostethic infection of the knee megaprosthesis and the use of external fixator during a two-stage revision. MATERIAL AND METHODS Between 01/1993 and 12/2013, a total of 67 cemented megaprostheses were assessed, with a detailed analysis of 12 patients with periprosthetic infection...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28154551/prolonged-activation-of-invariant-natural-killer-t-cells-and-th2-skewed-immunity-in-stroke-patients
#9
Connie H Y Wong, Craig N Jenne, Patrick P Tam, Caroline Léger, Andres Venegas, Karla Ryckborst, Michael D Hill, Paul Kubes
BACKGROUND: Infection is highly prevalent and contribute significantly to mortality of stroke patients. In addition to the well described robust systemic lymphocytopenia and skewed T helper 2 (TH2)-immunity after stroke, emerging experimental evidence demonstrate that the development of infection poststroke is attributed by the activation of invariant natural killer T (iNKT) cells. In this prospective study, we examined the levels of a broad spectrum of inflammatory mediators, the activation status of iNKT cell in the blood of patients with various degree of stroke severity, and investigate whether these parameters differ in patients who later develop poststroke infections...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28026725/-operative-treatment-for-saddle-shaped-femoral-heads
#10
M Burian, P Dungl, J Chomiak, M Ošťádal, R Kubeš, O Schwarz
PURPOSE OF THE STUDY A saddle-shaped deformity of the femoral head has a poor prognosis due to rapid development of secondary changes. A new method of treatment by intra-articular anteromedial wedge reduction osteotomy (AWRO) of the femoral head may preserve the hip for the future. This study was designed to ascertain that this invasive technique was safe and effective and to confirm our hypothesis that AWRO significantly improved functional and radiological parameters of the hip joint. MATERIAL AND METHODS Patients who underwent AWRO between 2010 and 2013 were enrolled in this study...
2016: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/27914376/mode-converted-ultrasonic-scattering-in-polycrystals-with-elongated-grains
#11
Andrea P Arguelles, Christopher M Kube, Ping Hu, Joseph A Turner
Elastic wave scattering is used to study polycrystalline media for a wide range of applications. Received signals, which include scattering from the randomly oriented grains comprising the polycrystal, contain information from which useful microstructural parameters may often be inferred. Recently, a mode-converted diffuse ultrasonic scattering model was developed for evaluating the scattered response of a transverse wave from an incident longitudinal wave in a polycrystalline medium containing equiaxed single-phase grains with cubic elastic symmetry...
September 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27699807/diversity-and-linkage-disequilibrium-in-farmed-tasmanian-atlantic-salmon
#12
J Kijas, N Elliot, P Kube, B Evans, N Botwright, H King, C R Primmer, K Verbyla
Farmed Atlantic salmon (Salmo salar) is a globally important production species, including in Australia where breeding and selection has been in progress since the 1960s. The recent development of SNP genotyping platforms means genome-wide association and genomic prediction can now be implemented to speed genetic gain. As a precursor, this study collected genotypes at 218 132 SNPs in 777 fish from a Tasmanian breeding population to assess levels of genetic diversity, the strength of linkage disequilibrium (LD) and imputation accuracy...
April 2017: Animal Genetics
https://www.readbyqxmd.com/read/27677377/an-evaluation-of-pre-hospital-emergency-medical-systems-for-suspected-st-elevation-myocardial-infarction-in-colorado
#13
Glenn H Engelman, Patrick M Carry, Kyle M Kubes, Michael J Gleason
OBJECTIVES: Patients presenting with ST-elevation myocardial infarction (STEMI) benefit from rapid cardiac reperfusion therapy. Emergency medical service (EMS) agencies can improve patient outcomes by calling STEMI alerts to the receiving facility. The aim of this study was to evaluate the use of pre-hospital activation systems for suspected ST-elevation myocardial infarctions (STEMI) throughout Colorado. METHODS: A cross sectional, survey design was utilized to collect all data from EMS agencies in Colorado...
November 2016: Postgraduate Medicine
https://www.readbyqxmd.com/read/27535218/microenvironmental-interactions-between-endothelial-and-lymphoma-cells-a-role-for-the-canonical-wnt-pathway-in-hodgkin-lymphoma
#14
F Linke, M Harenberg, M M Nietert, S Zaunig, F von Bonin, A Arlt, M Szczepanowski, H A Weich, S Lutz, C Dullin, P Janovská, M Krafčíková, L Trantírek, P Ovesná, W Klapper, T Beissbarth, F Alves, V Bryja, L Trümper, J Wilting, D Kube
The interaction between vascular endothelial cells (ECs) and cancer cells is of vital importance to understand tumor dissemination. A paradigmatic cancer to study cell-cell interactions is classical Hodgkin Lymphoma (cHL) owing to its complex microenvironment. The role of the interplay between cHL and ECs remains poorly understood. Here we identify canonical WNT pathway activity as important for the mutual interactions between cHL cells and ECs. We demonstrate that local canonical WNT signaling activates cHL cell chemotaxis toward ECs, adhesion to EC layers and cell invasion using not only the Wnt-inhibitor Dickkopf, tankyrases and casein kinase 1 inhibitors but also knockdown of the lymphocyte enhancer binding-factor 1 (LEF-1) and β-catenin in cHL cells...
February 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/27270428/wnt5a-a-motility-promoting-factor-in-hodgkin-lymphoma
#15
F Linke, S Zaunig, M M Nietert, F von Bonin, S Lutz, C Dullin, P Janovská, T Beissbarth, F Alves, W Klapper, V Bryja, T Pukrop, L Trümper, J Wilting, D Kube
Classical Hodgkin lymphoma (cHL) has a typical clinical manifestation, with dissemination involving functionally neighboring lymph nodes. The factors involved in the spread of lymphoma cells are poorly understood. Here we show that cHL cell lines migrate with higher rates compared with non-Hodgkin lymphoma cell lines. cHL cell migration, invasion and adhesion depend on autocrine WNT signaling as revealed by the inhibition of WNT secretion with the porcupine inhibitors Wnt-C59/IWP-2, but did not affect cell proliferation...
June 6, 2016: Oncogene
https://www.readbyqxmd.com/read/27250406/atmospheric-pressure-x-ray-photoelectron-spectroscopy-apparatus-bridging-the-pressure-gap
#16
J J Velasco-Vélez, V Pfeifer, M Hävecker, R Wang, A Centeno, A Zurutuza, G Algara-Siller, E Stotz, K Skorupska, D Teschner, P Kube, P Braeuninger-Weimer, S Hofmann, R Schlögl, A Knop-Gericke
One of the main goals in catalysis is the characterization of solid/gas interfaces in a reaction environment. The electronic structure and chemical composition of surfaces become heavily influenced by the surrounding environment. However, the lack of surface sensitive techniques that are able to monitor these modifications under high pressure conditions hinders the understanding of such processes. This limitation is known throughout the community as the "pressure gap." We have developed a novel experimental setup that provides chemical information on a molecular level under atmospheric pressure and in presence of reactive gases and at elevated temperatures...
May 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/26310202/ex-vivo-intra-arterial-methylene-blue-injection-in-rectal-cancer-specimens-increases-the-lymph-node-harvest-especially-after-preoperative-radiation
#17
Maria Münster, Uwe Hanisch, Muin Tuffaha, Rainer Kube, Henry Ptok
BACKGROUND: The examination of as large a number of lymph nodes as possible in rectal carcinoma resectates is important for exact staging. However, after neoadjuvant radiochemotherapy (RCT), it can be difficult to obtain a sufficient number of lymph nodes. We therefore investigated whether staining with methylene blue via the inferior mesenteric artery can lead to an increase in the yield of lymph nodes in rectal carcinoma tissue after neoadjuvant RCT. METHODS: In a prospective, unicentric study rectal carcinoma resectates from three consecutive groups of patients were examined (Group I, no staining; Group II, staining with methylene blue; Group III, again no staining)...
February 2016: World Journal of Surgery
https://www.readbyqxmd.com/read/26242222/pharmacokinetics-and-pharmacogenetics-of-capecitabine-and-its-metabolites-following-replicate-administration-of-two-500-mg-tablet-formulations
#18
RANDOMIZED CONTROLLED TRIAL
Christian Queckenberg, V Erlinghagen, B C M Baken, S H G Van Os, M Wargenau, V Kubeš, R Peroutka, V Novotný, U Fuhr
PURPOSE: To describe concentration versus time profiles of capecitabine and its metabolites 5'-DFUR, 5'-DFCR and 5-FU, depending on tablet formulation and on frequent and/or relevant genetic polymorphisms of cytidine deaminase, dihydropyrimidine dehydrogenase, thymidylate synthase and methylenetetrahydrofolate reductase (MTHFR). METHODS: In 46 cancer patients on chronic capecitabine treatment, who voluntarily participated in the study, individual therapeutic doses were replaced on four consecutive mornings by the study medication...
November 2015: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/26223451/-candidatus-phytoplasma-phoenicium-associated-with-almond-witches-broom-disease-from-draft-genome-to-genetic-diversity-among-strain-populations
#19
Fabio Quaglino, Michael Kube, Maan Jawhari, Yusuf Abou-Jawdah, Christin Siewert, Elia Choueiri, Hana Sobh, Paola Casati, Rosemarie Tedeschi, Marina Molino Lova, Alberto Alma, Piero Attilio Bianco
BACKGROUND: Almond witches'-broom (AlmWB), a devastating disease of almond, peach and nectarine in Lebanon, is associated with 'Candidatus Phytoplasma phoenicium'. In the present study, we generated a draft genome sequence of 'Ca. P. phoenicium' strain SA213, representative of phytoplasma strain populations from different host plants, and determined the genetic diversity among phytoplasma strain populations by phylogenetic analyses of 16S rRNA, groEL, tufB and inmp gene sequences. RESULTS: Sequence-based typing and phylogenetic analysis of the gene inmp, coding an integral membrane protein, distinguished AlmWB-associated phytoplasma strains originating from diverse host plants, whereas their 16S rRNA, tufB and groEL genes shared 100 % sequence identity...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/26167198/nervous-system-development-in-lecithotrophic-larval-and-juvenile-stages-of-the-annelid-capitella-teleta
#20
Néva P Meyer, Allan Carrillo-Baltodano, Richard E Moore, Elaine C Seaver
BACKGROUND: Reconstructing the evolutionary history of nervous systems requires an understanding of their architecture and development across diverse taxa. The spiralians encompass diverse body plans and organ systems, and within the spiralians, annelids exhibit a variety of morphologies, life histories, feeding modes and associated nervous systems, making them an ideal group for studying evolution of nervous systems. RESULTS: We describe nervous system development in the annelid Capitella teleta (Blake JA, Grassle JP, Eckelbarger KJ...
2015: Frontiers in Zoology
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