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M L V Azevedo, N B Bonan, G Dias, F Brehm, T M Steiner, W M Souza, A E M Stinghen, F C Barreto, Selene Elifio-Esposito, R Pecoits-Filho, A N Moreno-Amaral
Immune system dysfunction is a common condition in chronic kidney disease (CKD). The present study investigated the effect of p-Cresyl sulfate (pCS) on human cell line U937 monocyte-derived macrophages (MDM) activity. MDM (1×10(6) cells/mL) were incubated with pCS (10, 25, or 50μg/mL), with or without lipopolysaccharide (LPS; 25ng/mL) and then evaluated NO production, phagocytosis and antigen-presenting molecules expression (HLA-ABC, HLA-DR, CD80 and CD86). All analyses were performed by flow cytometry. All pCS concentrations were able to increase NO production (49±12...
October 16, 2016: Toxicology Letters
Wolfgang Saeger, Werner Mohren, Matthias Behrend, Peter Iglauer, Waldemar Wilczak
A tumor in the adrenal region with two metastases in the liver was classified as poorly differentiated sarcoma on the base of extensive immunostainings (expression of vimentin, desmin, myogenin, and CD31, no expression of inhibin, melan A). Four years later in a second examination with molecular methods for a study of adrenal sarcomas, this diagnosis must be revised due to the lack of MDM-2 gene amplification and FKHR translocation which exclude sarcoma. Further immunostainings of many other parts of the tumor showed in one area more mature tumor tissue expressing synaptophysin, SF-1, and melan A...
September 29, 2016: Endocrine Pathology
Martin A Bewley, Kylie B R Belchamber, Kirandeep K Chana, Richard C Budd, Gavin Donaldson, Jadwiga A Wedzicha, Christopher E Brightling, Iain Kilty, Louise E Donnelly, Peter J Barnes, Dave Singh, Moira K B Whyte, David H Dockrell
Pulmonary inflammation and bacterial colonization are central to the pathogenesis of chronic obstructive pulmonary disease (COPD). Defects in macrophage phagocytosis of both bacteria and apoptotic cells contribute to the COPD phenotype. Small molecule inhibitors with anti-inflammatory activity against p38 mitogen activated protein kinases (MAPKs), phosphatidyl-inositol-3 kinase (PI3K) and Rho kinase (ROCK) are being investigated as novel therapeutics in COPD. Concerns exist, however, about off-target effects...
2016: PloS One
Zhongying Wu, Juhao Li, Dawei Ge, Fang Ren, Paikun Zhu, Qi Mo, Zhengbin Li, Zhangyuan Chen, Yongqi He
Mode division multiplexing (MDM) has been widely investigated in optical transmission systems and networks to improve network capacity. However, the MDM receiver is always expensive and complex because coherent detection and multiplex-input-and-multiplex-output (MIMO) digital signal processing (DSP) are required to demultiplex each spatial mode. In this paper, we investigate the application of MDM in short-reach scenarios such as datacenter networking. Two-dimensional MDM and wavelength division multiplexing node structure based on low modal-crosstalk few-mode fiber (FMF) and components is proposed, in which signal in each mode or wavelength can be independently switched...
September 19, 2016: Optics Express
Rebecca Voltan, Erika Rimondi, Elisabetta Melloni, Gian Matteo Rigolin, Fabio Casciano, Maria Vittoria Arcidiacono, Claudio Celeghini, Antonio Cuneo, Giorgio Zauli, Paola Secchiero
OBJECTIVE: The aim of this study was to investigate the anti-leukemic activity of the Bruton tyrosine kinase inhibitor Ibrutinib in combination with the small molecule MDM-2 inhibitor Nutlin-3 in preclinical models. METHODS: The potential efficacy of the Ibrutinib/Nutlin-3 combination was evaluated in vitro in a panel of B leukemic cell lines (EHEB, JVM-2, JVM-3, MEC-1, MEC-2) and in primary B-chronic lymphocytic leukemia (B-CLL) patient samples, by assessing cell viability, cell cycle profile, apoptosis and intracellular pathway modulations...
September 20, 2016: Oncotarget
Walter Chingwaru, Richard H Glashoff, Jerneja Vidmar, Petrina Kapewangolo, Samantha L Sampson
Mycobacterium tuberculosis and human immunodeficiency virus (HIV) co-infections have remained a major public health concern worldwide, particularly in Southern Africa. Yet our understanding of the molecular interactions between the pathogens has remained poor due to lack of suitable preclinical models for such studies. We reviewed the use, this far, of mammalian cell culture models in HIV-MTB interaction studies. Studies have described the use of primary human cell cultures, including (1) monocyte-derived macrophage (MDM) fractions of peripheral blood mononuclear cell (PBMC), alveolar macrophages (AM), (2) cell lines such as the monocyte-derived macrophage cell line (U937), T lymphocyte cell lines (CEMx174, ESAT-6-specific CD4(+) T-cells) and an alveolar epithelial cell line (A549) and (3) special models such as stem cells, three dimensional (3D) or organoid cell models (including a blood-brain barrier cell model) in HIV-MTB interaction studies...
September 2016: Asian Pacific Journal of Tropical Medicine
Amir Sabet, Ina Binse, Semih Dogan, Andrea Koch, Sandra J Rosenbaum-Krumme, Hans-Jürgen Biersack, Kim Biermann, Samer Ezziddin
AIM: Distant metastasis has a negative impact on survival in differentiated thyroid carcinoma (DTC). The timing of this manifestation, however, is of unknown prognostic relevance. The aim of this retrospective study was to investigate the potential significance of discriminating synchronous versus metachronous distant metastases (SDM vs. MDM) for the outcome of patients with DTC. METHODS: We retrospectively analyzed a consecutive cohort of n = 89 patients with distant metastases of DTC (43 with follicular, 46 with papillary DTC histology; mean age 52...
August 16, 2016: European Journal of Nuclear Medicine and Molecular Imaging
Achim Troja, Hans-Günther Hempen, Mareike Demmer, Dalibor Antolovic, Hans-Rudolf Raab
BACKGROUND: Tumor response after neoadjuvant radiochemotherapy (NRC) prior to surgery and other parameters are likely to have an influence on the survival rate of patients suffering from T3 rectal cancer. METHODS: 51 patients (17 female, 34 male; 59.0 years; Apache < 9 points: 95.1%; ASA I-II 88.3% and ASA III 11.8%) were treated with NRC (50.4 Gy; 5-fluorouracil/folinic acid) 4-6 weeks prior to surgery because of uT3 rectal cancer (G2: 96%; adenocarcinoma 86...
April 2016: Visc Med
Motoaki Ohnaka, Masahisa Kyogoku, Hiroyuki Nakajima, Hiroaki Osada, Katsuaki Meshii, Naoki Kanemitsu
Primary intimal sarcoma of the heart is an extremely rare tumor that is known to have a very poor prognosis. We present a case of a 65-year-old man who suffered from deteriorating congestive heart failure due to a severe mitral stenosis caused by a large mobile left atrial tumor. The patient underwent an emergency operation of the tumor in the left atrium. The tumor was attached to the inferior wall of the left atrium. After the resection of the tumor, a second tumor on the interatrial septum, which had not been detected in the preoperative investigation, was discovered and resected...
September 2016: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Junpeng Liang, Qi Mo, Songnian Fu, Ming Tang, P Shum, Deming Liu
We propose a design strategy of elliptical core few-mode fiber (e-FMF) that supports three spatial modes with enhanced mode spacing between LP<sub>11a</sub> and LP<sub>11b</sub>, to suppress intra-mode coupling during mode-division multiplexing (MDM) transmission. Our theoretical investigations show that there exist two optimization regimes for the e-FMF, as a comparison with traditional circular core FMF(c-FMF). At the regime of three-mode operation, there occurs a trade-off between mode spacing and bending-induced loss...
July 1, 2016: Optics Letters
Joana R Guedes, Isabel Santana, Catarina Cunha, Diana Duro, Maria R Almeida, Ana M Cardoso, Maria C Pedroso de Lima, Ana L Cardoso
INTRODUCTION: Mononuclear phagocytes play a critical role during Alzheimer's disease (AD) pathogenesis due to their contribution to innate immune responses and amyloid beta (Aβ) clearance mechanisms. METHODS: Blood-derived monocytes (BDMs) and monocyte-derived macrophages (MDMs) were isolated from blood of AD, mild cognitive impairment (MCI) patients, and age-matched healthy controls for molecular and phenotypic comparisons. RESULTS: The chemokine/chemokine receptor CCL2/CCR2 axis was impaired in BDMs from AD and MCI patients, causing a deficit in cell migration...
2016: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
Yu Cong, Monica A McArthur, Melanie Cohen, Peter B Jahrling, Krisztina B Janosko, Nicole Josleyn, Kai Kang, Tengfei Zhang, Michael R Holbrook
Humans infected with yellow fever virus (YFV), a mosquito-borne flavivirus, can develop illness ranging from a mild febrile disease to hemorrhagic fever and death. The 17D vaccine strain of YFV was developed in the 1930s, has been used continuously since development and has proven very effective. Genetic differences between vaccine and wild-type viruses are few, yet viral or host mechanisms associated with protection or disease are not fully understood. Over the past 20 years, a number of cases of vaccine-associated disease have been identified following vaccination with 17D; these cases have been correlated with reduced immune status at the time of vaccination...
May 2016: PLoS Neglected Tropical Diseases
Wei Chang, Wei He, Pei-Pei Li, Sha-Sha Song, Ping-Fan Yuan, Jing-Tao Lu, Wei Wei
Celastrol, an active ingredient of Tripterygium Wilfordii, is a traditional Chinese medicinal herb, which has attracted interests for its potential anti-inflammatory and anti-cancer activities. The aim of this study was to evaluate the anti-tumor effect of Celastrol against diethylnitrosamine (DEN)-induced hepatocellular carcinoma (HCC) in rats and furthermore, to explore the underlying mechanism. Sprague-Dawley rats were intragastrically administered with DEN (10mg/kg) for 6 days every week and persisting 16 weeks...
August 5, 2016: European Journal of Pharmacology
Jiaxin Gong, Jing Xu, Ming Luo, Xiang Li, Ying Qiu, Qi Yang, Xinliang Zhang, Shaohua Yu
We report in this work the first all-optical wavelength conversion (AOWC) of a mode division multiplexed (MDM) superchannel consisting of 2N modes by dividing the superchannel into N single-mode (SM) tributaries, wavelength converting N SM signals using well developed SM-AOWC techniques, and finally combining the N SM tributaries back to an MDM superchannel at the converted wavelength, inspired by the idea of using SM filtering techniques to filter multimode signals in astronomy. The conversions between multimode and SM are realized by 3D laser-writing photonic lanterns and SM-AOWCs are realized based on polarization insensitive four wave mixing (FWM) configuration in N semiconductor optical amplifiers (SOAs)...
April 18, 2016: Optics Express
Gerald Julius Wiechmann, Erol Saygili, Christoph Zilkens, Rüdiger Krauspe, Michael Behringer
The aim of this paper is to identify predictors of serum muscle damage marker (MDM) response following mixed martial arts (MMA) matches. Creatine kinase activity (CK) and myoglobin concentration (Mb) were measured in ten male elite MMA fighters (aged 28±5.7 years) prior to, 2 h, 24 h, and 96 h following 9 different MMA matches. The number of performed upright punches and kicks (UKF) that failed the opponent, the number of obtained hits to the upper and lower body (LBH), as well as the total fight duration (TFD) were evaluated as potential predictors from video recordings...
March 21, 2016: Orthopedic Reviews
Ngoan Tran Le, Fernanda Alessandra Silva Michels, Mingyang Song, Xuehong Zhang, Adam M Bernstein, Edward L Giovannucci, Charles S Fuchs, Shuji Ogino, Andrew T Chan, Rashmi Sinha, Walter C Willett, Kana Wu
BACKGROUND: Heterocyclic amines (HCAs) in cooked meats may play a role in colorectal cancer (CRC) development. OBJECTIVES: To prospectively examine the association between estimated intakes of HCAs and meat derived mutagenicity (MDM) in two cohorts of health professionals, the Health Professionals Follow-up Study (HPFS) and the Nurses' Health Study (NHS). METHODS: In 29,615 men and 65,875 women, intake of the HCAs 2-amino-3,8-dimethylimidazo quinoxaline (MeIQx), 2-amino-1-methyl-6-phenylimidazo pyridine (PhIP), 2-amino-3,4,8-trimethylimidazo (DiMeIQx) and MDM was estimated using a 1996 cooking questionnaire, the 1994 food frequency questionnaire and an online database...
April 22, 2016: Environmental Health Perspectives
Shrikant R Mulay, Simone Romoli, Jyaysi Desai, Mohammad Mohsen Honarpisheh, Santhosh V Kumar, Hans-Joachim Anders, Dana Thomasova
Rapidly progressive glomerulonephritis is characterized by glomerular necroinflammation and crescent formation. Its treatment includes unspecific and toxic agents; therefore, the identification of novel therapeutic targets is required. The E3-ubiquitin ligase murine double minute (MDM)-2 is a nonredundant element of NF-κB signaling and the negative regulator of tumor suppressor gene TP53-mediated cell cycle arrest and cell death. We hypothesized that the MDM2 would drive crescentic glomerulonephritis by NF-κB-dependent glomerular inflammation and by p53-dependent parietal epithelial cell hyperproliferation...
June 2016: American Journal of Pathology
Jenny Harris, Cath Taylor, Nick Sevdalis, Rozh Jalil, James S A Green
OBJECTIVE: To develop a tool for independent observational assessment of cancer multidisciplinary team meetings (MDMs), and test criterion validity, inter-rater reliability/agreement and describe performance. DESIGN: Clinicians and experts in teamwork used a mixed-methods approach to develop and refine the tool. Study 1 observers rated pre-determined optimal/sub-optimal MDM film excerpts and Study 2 observers independently rated video-recordings of 10 MDMs. SETTING: Study 2 included 10 cancer MDMs in England...
June 2016: International Journal for Quality in Health Care
Yong-Taek Jung, Sooyeon Park, Jung-Sook Lee, Jung-Hoon Yoon
A Gram-stain-negative, coccoid- or short-rods-shaped and non-motile bacterial strain, designated MDM-7T was isolated from seawater of Yellow Sea, South Korea, and was subjected to a polyphasic taxonomic study. Strain MDM-7T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-7T fell within the clade comprising the Loktanella species, clustering with the type strains of Loktanella vestfoldensis and Loktanella agnita, with which it exhibited 96...
April 15, 2016: International Journal of Systematic and Evolutionary Microbiology
Simona Daniele, Valeria La Pietra, Elisabetta Barresi, Salvatore Di Maro, Eleonora Da Pozzo, Marco Robello, Concettina La Motta, Sandro Cosconati, Sabrina Taliani, Luciana Marinelli, Ettore Novellino, Claudia Martini, Federico Da Settimo
In glioblastoma multiforme (GBM), translocator protein (TSPO) and murine double minute (MDM)2/p53 complex represent two druggable targets. We recently reported the first dual binder 3 possessing a higher anticancer effect in GBM cells than the standards PK11195 1 or Nutlin-3 2 singularly applied. Herein, through a structure-activity relationship study, we developed derivatives 4-10 with improved potencies toward both TSPO and MDM2. As a result, compound 9: (i) reactivated the p53 functionality; (ii) inhibited the viability of two human GBM cells; (iii) impaired the proliferation of glioma cancer stem cells (CSCs), more resistant to chemotherapeutics and responsible of GBM recurrence; (iv) sensitized GBM cells and CSCs to the activity of temozolomide; (v) directed its effects preferentially toward tumor cells with respect to healthy ones...
May 26, 2016: Journal of Medicinal Chemistry
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