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https://www.readbyqxmd.com/read/29298131/a-systematic-review-of-p53-regulation-of-oxidative-stress-in-skeletal-muscle
#1
Kaitlyn Beyfuss, David A Hood
BACKGROUND: p53 is a tumor suppressor protein involved in regulating a wide array of signaling pathways. The role of p53 in the cell is determined by the type of imposed oxidative stress, its intensity and duration. The last decade of research has unravelled a dual nature in the function of p53 in mediating the oxidative stress burden. However, this is dependent on the specific properties of the applied stress and thus requires further analysis. METHODS: A systematic review was performed following an electronic search of Pubmed, Google Scholar, and ScienceDirect databases...
January 3, 2018: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/29260730/measurement-of-effective-detective-quantum-efficiency-for-a-photon-counting-scanning-mammography-system-and-comparison-with-two-flat-panel-full-field-digital-mammography-systems
#2
Tim J Wood, Craig S Moore, John R Saunderson, Andy W Beavis
Effective Detective Quantum Efficiency (eDQE) describes the resolution and noise properties of an imaging system along with scatter and primary transmission, all measured under clinically appropriate conditions. Effective dose efficiency (eDE) is the eDQE normalised to mean glandular dose and has been proposed as a useful metric for the optimisation of clinical imaging systems. The aim of this study was to develop a methodology for measuring eDQE and eDE on a Philips MicroDose Mammography (MDM) L30 photon counting scanning system, and to compare performance with two conventional flat panel systems...
December 20, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29230224/lipoarabinomannan-decreases-galectin-9-expression-and-tumor-necrosis-factor-pathway-in-macrophages-favoring-mycobacterium-tuberculosis-intracellular-growth
#3
Leslie Chávez-Galán, Lucero Ramon-Luing, Claudia Carranza, Irene Garcia, Isabel Sada-Ovalle
Lipoarabinomannan (LAM) is a lipid virulent factor secreted by Mycobacterium tuberculosis (Mtb). LAM can be found in the sputum and urine of patients with active tuberculosis. When human monocytes are differentiated into macrophages [monocyte-derived macrophages (MDM)] in the presence of LAM, MDM are poorly functional which may limit the immune response to Mtb infection. Our previous studies have shown that TIM3 and galectin (GAL)9 interaction induces anti-mycobacterial activity, and the expression levels of TIM3 and GAL9 are downregulated during Mtb infection...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29170044/differential-gene-expression-in-mycobacterium-bovis-challenged-monocyte-derived-macrophages-of-cattle
#4
Sanjeev Kumar Shukla, Shubhra Shukla, Anuj Chauhan, Sarvjeet, Rehan Khan, Lakshya Veer Singh, Naveen Sharma, Chandan Prakash, Ajay Vir Singh, Manjit Panigrahi
A functional genomics approach was used to examine the immune response for transcriptional profiling of PBMC M. bovis infected cattle and healthy control cattle to stimulation with bovine tuberculin (purified protein derivative PPD-b). Total cellular RNA was extracted from non-challenged control and M. bovis challenged MDM for all animals at intervals of 6 h post-challenge, in response to in-vitro challenge with M. bovis (multiplicity of infection 2:1) and prepared for global gene expression analysis using the Agilent Bovine (V2) Gene Expression Microarray, 8 × 60 K...
November 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29139175/hematopoietic-substrate-1-associated-protein-x-1-regulates-the-proliferation-and-apoptosis-of-endothelial-progenitor-cells-through-akt-pathway-modulation
#5
Xin-Bin Guo, Xin Deng, Ying Wei
Endothelial precursor cells (EPCs) are involved in vasculogenesis of various physiological and pathological processes. The proliferation and survival mechanism of EPCs needs to be explored further for the purpose of developing an effective glioma treatment. Hematopoietic substrate-1-associated protein X-1 (HAX-1) has been reported as an anti-apoptotic protein that plays an important role in several malignant tumors. However, the effect and mechanism of HAX-1 on EPCs remains unknown. This study aims to investigate the effect of HAX-1 on the proliferation and apoptosis of EPCs and explore its mechanism...
November 15, 2017: Stem Cells
https://www.readbyqxmd.com/read/29060815/prediction-of-fatigue-related-driver-performance-from-eeg-data-by-deep-riemannian-model
#6
Mehdi Hajinoroozi, Jianqiu Zhang, Yufei Huang
Prediction of the drivers' drowsy and alert states is important for safety purposes. The prediction of drivers' drowsy and alert states from electroencephalography (EEG) using shallow and deep Riemannian methods is presented. For shallow Riemannian methods, the minimum distance to Riemannian mean (mdm) and Log-Euclidian metric are investigated, where it is shown that Log-Euclidian metric outperforms the mdm algorithm. In addition the SPDNet, a deep Riemannian model, that takes the EEG covariance matrix as the input is investigated...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29049737/introduction-of-the-mdm2-t309g-mutation-in-primary-human-retinal-epithelial-cells-enhances-experimental-proliferative-vitreoretinopathy
#7
Guohong Zhou, Yajiang Duan, Gaoen Ma, Wenyi Wu, Zhengping Hu, Na Chen, Yewlin Chee, Jing Cui, Arif Samad, Joanne A Matsubara, Shizuo Mukai, Patricia A D'Amore, Hetian Lei
Purpose: The murine double minute (MDM)2 is a critical negative regulator of the p53 tumor suppressor, and MDM2 SNP309G is associated with a higher risk of proliferative vitreoretinopathy (PVR); in addition, the MDM2 T309G created using clustered regularly interspaced short palindromic repeats (CRISPR)/associated endonuclease (Cas)9 enhances normal rabbit vitreous-induced expression of MDM2 and survival of primary human retinal pigment epithelial (hRPE) cells in vitro. The goal of this study was to determine whether this MDM2 T309G contributes to the development of experimental PVR...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29047882/method-proposing-a-slow-light-ring-resonator-structure-coupled-with-a-metal-dielectric-metal-waveguide-system-based-on-plasmonic-induced-transparency
#8
Mehdi Hassani Keleshtery, Hassan Kaatuzian, Ali Mir, Ashkan Zandi
We demonstrate the analogue of electromagnetically induced transparency (EIT) in a metal-dielectric-metal (MDM) plasmonic waveguide. Plasmonic induced transparency is a method similar to EIT. In this paper, a plasmonic MDM waveguide is proposed by using an ellipse shaped side-coupled ring resonator and simulated by finite difference time domain. Plasmonics as a new field of chip-scale technology is an interesting substrate, which is used to propose and numerically investigate a novel MDM structure. The aforementioned framework is a 2×2 plasmonic ring resonator, employing gold as a metal and polymethyl methacrylate as a dielectric...
May 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/29046677/defining-genome-wide-expression-and-phenotypic-contextual-cues-in-macrophages-generated-by-granulocyte-macrophage-colony-stimulating-factor-macrophage-colony-stimulating-factor-and-heat-killed-mycobacteria
#9
Samer Bazzi, Emale El-Darzi, Tina McDowell, Helmout Modjtahedi, Satvinder Mudan, Marcel Achkar, Charles Akle, Humam Kadara, Georges M Bahr
Heat-killed (HK) Mycobacterium obuense (NCTC13365) is currently being evaluated in the clinic as an immunotherapeutic agent for cancer treatment. Yet, the molecular underpinnings underlying immunomodulatory properties of HK M. obuense are still largely undefined. To fill this void, we sought to perform immunophenotyping, chemokine/cytokine release analysis and genome-wide characterization of monocyte-derived macrophages (MDM) in which monocytes were originally isolated from healthy donors and differentiated by HK M...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29022913/mbd2-upregulates-mir-301a-5p-to-induce-kidney-cell-apoptosis-during-vancomycin-induced-aki
#10
Juan Wang, Huiling Li, Shuangfa Qiu, Zheng Dong, Xudong Xiang, Dongshan Zhang
Despite DNA methylation occurred in acute kidney injury (AKI), how it influenced progression of AKI remains unclear. Methyl-CpG-binding domain protein 2 (MBD2), a protein readers of methylation, was used to analyze the impact of DNA methylation on vancomycin (VAN)-induced AKI. Here, in cultured human kidney tubular epithelial cells (HK-2), we show that knockdown of MBD2 by siRNA attenuated VAN-induced apoptosis, caspase activity, and the expression of BAX and cleaved caspase 3. Interestingly, knockdown of MBD2 by siRNA was associated with the suppression of miR-301a-5p...
October 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28956865/effects-of-amprenavir-on-hiv-1-maturation-production-and-infectivity-following-drug-withdrawal-in-chronically-infected-monocytes-macrophages
#11
Ana Borrajo, Alessandro Ranazzi, Michela Pollicita, Rosalinda Bruno, Andrea Modesti, Claudia Alteri, Carlo Federico Perno, Valentina Svicher, Stefano Aquaro
A paucity of information is available on the activity of protease inhibitors (PI) in chronically-infected monocyte-derived macrophages (MDM) and on the kinetics of viral-rebound after PI removal in vitro. To fill this gap, the activity of different concentrations of amprenavir (AMP) was evaluated in chronically-infected MDM by measuring p24-production every day up to 12 days after drug administration and up to seven days after drug removal. Clinically-relevant concentrations of AMP (4 and 20 μM) drastically decreased p24 amount released from chronically-infected MDM from Day 2 up to Day 12 after drug administration...
September 28, 2017: Viruses
https://www.readbyqxmd.com/read/28921940/attribution-of-human-infections-with-shiga-toxin-producing-escherichia-coli-stec-to-livestock-sources-and-identification-of-source-specific-risk-factors-the-netherlands-2010-2014
#12
L Mughini-Gras, W van Pelt, M van der Voort, M Heck, I Friesema, E Franz
Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen of public health concern whose sources and transmission routes are difficult to trace. Using a combined source attribution and case-control analysis, we determined the relative contributions of four putative livestock sources (cattle, small ruminants, pigs, poultry) to human STEC infections and their associated dietary, animal contact, temporal and socio-econo-demographic risk factors in the Netherlands in 2010/2011-2014. Dutch source data were supplemented with those from other European countries with similar STEC epidemiology...
September 17, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28796153/optimum-drug-combinations-for-the-sedation-of-growing-boars-prior-to-castration
#13
Heidi S Lehmann, Dominique Blache, Eleanor Drynan, Pema Tshewang, David J C Blignaut, Gabrielle C Musk
Juvenile male pigs were sedated for castration. Eight five-month old boars were sedated twice (two weeks apart) with a combination of detomidine (0.1 mg/kg), midazolam (0.2 mg/kg) and either butorphanol (0.2 mg/kg) (Group MDB, n = 8) or morphine (0.2 mg/kg) (Group MDM, n = 8) intramuscularly. The boars were positioned in lateral recumbency and lidocaine (200 mg total) was injected into the testicle and scrotal skin. Castration of a single testicle was performed on two occasions. Sedation and reaction (to positioning and surgery) scores, pulse rate, respiratory rate, haemoglobin oxygen saturation, body temperature, arterial blood gas parameters and the times to immobility and then recovery were recorded...
August 10, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28794126/a-refined-method-to-study-gene-dosage-changes-in-vitro-using-crispr-cas9
#14
Teresa P Raposo, Henry O Ebili, Mohammad Ilyas
AIMS: Gene dosage can have a major impact on cell biology, although, hitherto, it has been difficult to study using in vitro models. We sought to refine and accelerate the development of 'gene dosage' models through using CRISPR/Cas9 (a gene editing technology) for sequential knockout of gene alleles. METHODS: Our method involved (1) using Cas9 nuclease mRNA rather than expression plasmids, (2) using a fluorescently labelled FAM-6 tracr complexed with guide RNA and (3) using high-resolution melting (HRM) analysis to screen for mutations...
August 9, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28761130/lipodystrophy-and-obesity-are-associated-with-decreased-number-of-t-cells-with-regulatory-function-and-pro-inflammatory-macrophage-phenotype
#15
S Berger, G Ceccarini, G Scabia, I Barone, C Pelosini, F Ferrari, S Magno, A Dattilo, L Chiovato, P Vitti, F Santini, M Maffei
BACKGROUND/OBJECTIVES: In lipodystrophy (LD) adipose tissue function to store lipids is impaired, leading to metabolic syndrome, similar to that found in obesity. Emerging evidence links dysmetabolism with disorders of the immune system. Our aim is to investigate whether T-cell populations with regulatory function and monocyte-derived macrophages (MDMs) are affected by LD and obesity. SUBJECTS/METHODS: Blood was collected from 16 LD, 16 obese (OB, BMI>30 kg m(-2)) and 16 healthy normal-weight women (CNT)...
November 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28746408/microwave-magnetic-proteomics-of-macrophages-from-patients-with-hiv-associated-cognitive-impairment
#16
Yisel M Cantres-Rosario, Frances M Acevedo-Mariani, Juliana Pérez-Laspiur, William E Haskins, Marines Plaud, Yadira M Cantres-Rosario, Richard Skolasky, Israel Méndez-Bermúdez, Valerie Wojna, Loyda M Meléndez
OBJECTIVE: HIV-infected monocytes can infiltrate the blood brain barrier as differentiated macrophages to the central nervous system, becoming the primary source of viral and cellular neurotoxins. The final outcome is HIV-associated cognitive impairment (HACI), which remain prevalent today, possibly due to the longer life-span of the patients treated with combined anti-retroviral therapy. Our main goal was to characterize the proteome of monocyte-derived macrophages (MDM) from HACI patients, and its association with their cognitive status, to find novel targets for therapy...
2017: PloS One
https://www.readbyqxmd.com/read/28721957/nutritional-status-of-mid-day-meal-programme-beneficiaries-a-cross-sectional-study-among-primary-schoolchildren-in-kottayam-district-kerala-india
#17
Rajeev Jayalakshmi, Vinoda Thulaseedharan Jissa
BACKGROUND: The efficiency of Mid-Day Meal (MDM) Programme in India to ensure the optimum nutritional status of its beneficiaries is rarely studied. OBJECTIVE: This study assessed the nutritional status of 6-10-year-old schoolchildren who were the beneficiaries of MDM and the child-related factors affecting their nutritional status. METHODS: A cross-sectional study was performed among 322 children from 12 randomly selected primary schools in one block panchayat of Kerala state...
April 2017: Indian Journal of Public Health
https://www.readbyqxmd.com/read/28698313/hiv-and-hiv-tat-inhibit-lps-induced-il-27-production-in-human-macrophages-by-distinct-intracellular-signaling-pathways
#18
Niranjala Gajanayaka, Shifawn O'Hara, Yulia Konarski, Jason Fernandes, Kar Muthumani, Maya Kozlowski, Jonathan B Angel, Ashok Kumar
Monocyte-derived Mϕs (MDMs) from HIV-infected patients and MDM infected in vitro with HIV exhibit a reduced ability to secrete various cytokines, including IL-12. Recently, IL-27, an IL-12 family cytokine, was shown to inhibit HIV replication in Mϕ. Whether HIV infection or HIV accessory protein(s) impact IL-27 production in Mϕs remains unknown. Herein, we show that in vitro HIV infection, as well as intracellular HIV-Tat (Tat) and Tat peptides, inhibit LPS-induced IL-27 production in human MDMs, suggesting impairment of the TLR4 signaling pathway...
September 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28667027/synergy-between-paclitaxel-and-anti-cancer-peptide-pnc-27-in-the-treatment-of-ovarian-cancer
#19
Ioannis Alagkiozidis, Constantine Gorelick, Tana Shah, Yi-Ju Amy Chen, Vinita Gupta, Dimitre Stefanov, Abhi Amarnani, Yi-Chun Lee, Ovadia Abulafia, Ehsan Sarafraz-Yazdi, Josef Michl
OBJECTIVES: Paclitaxel is widely used in the treatment of gynecologic malignancies. It targets tumor cells in the M phase of the cell cycle. Cells in other phases survive the insult and repopulate the tumor. PNC-27 is a peptide synthesized of amino acids of the p53-MDM-2 binding domain. It kills various cancer cell lines in a dose-dependent manner. The goal of this study is to assess ovarian cancer cells' sensitivity to PNC-27 after surviving exposure to paclitaxel and to investigate the potential for synergy between PNC-27 and paclitaxel in the treatment of ovarian cancer...
May 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28557543/impaired-mitochondrial-microbicidal-responses-in-chronic-obstructive-pulmonary-disease-macrophages
#20
Martin A Bewley, Julie A Preston, Mohammed Mohasin, Helen M Marriott, Richard C Budd, Julie Swales, Paul Collini, David R Greaves, Ruth W Craig, Christopher E Brightling, Louise E Donnelly, Peter J Barnes, Dave Singh, Steven D Shapiro, Moira K B Whyte, David H Dockrell
RATIONALE: Chronic obstructive pulmonary disease (COPD) is characterized by impaired clearance of pulmonary bacteria. OBJECTIVES: The effect of COPD on alveolar macrophage (AM) microbicidal responses was investigated. METHODS: AMs were obtained from bronchoalveolar lavage from healthy donors or patients with COPD and challenged with opsonized serotype 14 Streptococcus pneumoniae. Cells were assessed for apoptosis, bactericidal activity, and mitochondrial reactive oxygen species (mROS) production...
October 1, 2017: American Journal of Respiratory and Critical Care Medicine
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