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https://www.readbyqxmd.com/read/28546635/local-cryotherapy-minimally-impacts-the-metabolome-and-transcriptome-of-human-skeletal-muscle
#1
Dylan C Sarver, Kristoffer B Sugg, Nathaniel P Disser, Elizabeth R Sibilsky Enselman, Tariq M Awan, Christopher L Mendias
Cryotherapy is commonly used in the treatment of skeletal muscle injuries. However, the data to support the use of cryotherapy is inconclusive, and the biochemical etiology of cryotherapy in human skeletal muscle remains largely unknown. We therefore sought to determine how a clinically-relevant dose of cryotherapy would impact the transcriptome and metabolome of skeletal muscle. Eight healthy male subjects (age 24.7 ± 4.5 years, BMI 22.2 ± 1.6) received a 15 minute bout of local cryotherapy, delivered via ice cup massage over the anterolateral thigh...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28546339/bmp-induced-reprograming-of-the-retina-into-rpe-requires-wnt-signalling-in-the-developing-chick-optic-cup
#2
Jörg Steinfeld, Ichie Steinfeld, Alexander Bausch, Nicola Coronato, Meggi-Lee Hampel, Heike Depner, Paul G Layer, Astrid Vogel-Höpker
In vertebrates, the retinal pigment epithelium (RPE) and photoreceptors of the neural retina (NR) comprise a functional unit required for vision. During vertebrate eye development, a conversion of the RPE into NR can be induced by growth factors in vivo at optic cup stages, but the reverse process, the conversion of NR tissue into RPE has not been reported. Here, we show that bone morphogenetic proteins (BMP) signalling can reprogram the NR into RPE at optic cup stages in chick. Shortly after BMP application, expression of Microphthalmia associated transcription factor (Mitf) is induced in the NR and selective cell death on the basal side of the NR induces an RPE-like morphology...
May 25, 2017: Biology Open
https://www.readbyqxmd.com/read/28545734/cup-to-disc-ratio-asymmetry-in-u-s-adults-prevalence-and-association-with-glaucoma-in-the-2005-2008-national-health-and-nutrition-examination%C3%A2-survey
#3
Mary Qiu, Michael V Boland, Pradeep Y Ramulu
PURPOSE: To describe the prevalence of vertical cup-to-disc ratio (vCDR) asymmetry in U.S. adults and assess the utility of vCDR asymmetry in the diagnosis of glaucoma. DESIGN: Cross-sectional study. PARTICIPANTS: A total of 5359 subjects aged ≥40 years from the 2005-2008 National Health and Nutrition Examination Survey (NHANES). METHODS: Subjects completed retinal photography and had vCDR determined in both eyes, with vCDR asymmetry calculated as the absolute value of the difference between the 2 eyes...
May 22, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28542710/behavioral-inferences-from-the-high-levels-of-dental-chipping-in-homo-naledi
#4
Ian Towle, Joel D Irish, Isabelle De Groote
OBJECTIVES: A variety of mechanical processes can result in antemortem dental chipping. In this study, chipping data in the teeth of Homo naledi are compared with those of other pertinent dental samples to give insight into their etiology. MATERIALS AND METHODS: Permanent teeth with complete crowns evidencing occlusal wear were examined macroscopically. The location, number, and severity of fractures were recorded and compared to those found in samples of two other South African fossil hominin species and in samples of nonhuman primates (n = 3) and recent humans (n = 7)...
May 24, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28542606/what-is-in-your-cup-of-tea-dna-verity-test-to-characterize-black-and-green-commercial-teas
#5
Olga De Castro, Maria Comparone, Antonietta Di Maio, Emanuele Del Guacchio, Bruno Menale, Jacopo Troisi, Francesco Aliberti, Marco Trifuoggi, Marco Guida
In this study, we used several molecular techniques to develop a fast and reliable protocol (DNA Verity Test, DVT) for the characterization and confirmation of the species or taxa present in herbal infusions. As a model plant for this protocol, Camellia sinensis, a traditional tea plant, was selected due to the following reasons: its historical popularity as a (healthy) beverage, its high selling value, the importation of barely recognizable raw product (i.e., crushed), and the scarcity of studies concerning adulterants or contamination...
2017: PloS One
https://www.readbyqxmd.com/read/28542504/level-of-surgical-experience-is-associated-with-change-in-hip-center-of-rotation-following-cementless-total-hip-arthroplasty-a-radiographic-assessment
#6
Seung-Chan Kim, Young-Wook Lim, Soon-Yong Kwon, Woo-Lam Jo, Sung-Hun Ju, Chan-Joo Park, Choong-Woo Lee, Yong-Sik Kim
OBJECTIVES: After total hip arthroplasty (THA), restoration of hip center of rotation (COR) is essential to ensure stability of the prosthetic hip and longevity of the prosthesis. Our aim was to determine whether, and how, the COR changed postoperatively compared to the native COR following implantation of a cementless acetabular component in anatomical position and to compare the accuracy of cup placement between two surgeons with different levels of surgical experience. MATERIALS AND METHODS: We evaluated 145 patients (145 hips) who underwent unilateral primary THA, who had no distorted acetabulum on the affected hip and a normal contralateral hip...
2017: PloS One
https://www.readbyqxmd.com/read/28542284/haemocytes-collected-from-experimentally-infected-pacific-oysters-crassostrea-gigas-detection-of-ostreid-herpesvirus-1-dna-rna-and-proteins-in-relation-with-inhibition-of-apoptosis
#7
Claire Martenot, Ophélie Gervais, Bruno Chollet, Maryline Houssin, Tristan Renault
Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this way, an OsHV-1 challenge was performed and oysters were monitored at three times post injection to investigate viral infection and host response: 2h (early after viral injection in the adductor muscle), 24h (intermediate time), and 48h (just before first oyster mortality record)...
2017: PloS One
https://www.readbyqxmd.com/read/28541143/an-algorithm-for-identifying-mothball-composition
#8
Michael J Moss, Kevin F Maskell, Michelle A Hieger, Brandon K Wills, Kirk L Cumpston
INTRODUCTION: Unintentional mothball ingestions may cause serious toxicity in small children. Camphor, naphthalene, and paradichlorobenzene mothballs are difficult to distinguish without packaging. Symptoms and management differ based on the ingested compound. Previous studies have used a variety of antiquated, impractical and potentially dangerous techniques to identify the mothballs. The goal of this study is to discover a simplified identification technique using materials readily available in an emergency department...
May 25, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28540004/the-association-of-central-corneal-thickness-with-ocular-and-general-parameters-in-a-community-setting-the-yazd-eye-study
#9
Reza Soleimanizad, Mohammad Hosein Nowroozzadeh, Hossein Ziaei, Mohammad Pakravan, Mehdi Yaseri, Marzieh Katibeh
PURPOSE: To assess central corneal thickness (CCT) and its associations in an adult Iranian population. METHODS: This was a population-based cross-sectional study of adults aged 40-80 years. Eyes with corneal disorders, previous ocular surgery, or trauma were excluded. All subjects underwent complete ophthalmic examination, general health assessment, laboratory tests, and a detailed interview. CCT was measured with an ultrasonic pachymeter. Intraocular pressure (IOP) was measured with Goldmann applanation tonometry...
April 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28539432/golgi-associated-protein-kinase-c-%C3%AE%C2%B5-is-delivered-to-phagocytic-cups-role-of-phosphatidylinositol-4-phosphate
#10
Cheryl M Hanes, Anna E D'Amico, Takehiko Ueyama, Alexander C Wong, Xuexin Zhang, W Frederick Hynes, Margarida M Barroso, Nathaniel C Cady, Mohamed Trebak, Naoaki Saito, Michelle R Lennartz
Protein kinase C-ε (PKC-ε) at phagocytic cups mediates the membrane fusion necessary for efficient IgG-mediated phagocytosis. The C1B and pseudosubstrate (εPS) domains are necessary and sufficient for this concentration. C1B binds diacylglycerol; the docking partner for εPS is unknown. Liposome assays revealed that the εPS binds phosphatidylinositol 4-phosphate (PI4P) and PI(3,5)P2 Wortmannin, but not LY294002, inhibits PKC-ε concentration at cups and significantly reduces the rate of phagocytosis. As Wortmannin inhibits PI4 kinase, we hypothesized that PI4P mediates the PKC-ε concentration at cups and the rate of phagocytosis...
May 24, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28538561/is-rotation-a-concern-with-anatomical-breast-implants-a-statistical-analysis-of-factors-predisposing-to-rotation
#11
Paolo Montemurro, Athanasios Papas, Per Hedén
BACKGROUND: Since their introduction in 1993, anatomical implants have provided a more natural appearance in breast augmentation, and many surgeons advocate their use and promote the good aesthetic results. However, the risk of implant rotation makes some of them reluctant to use these devices. The rotation rate varies among authors. METHODS: The authors present a 6.5-year series of 531 patients who underwent primary breast augmentation with macrotextured anatomical implants in a Swedish facility performed by one consultant surgeon (P...
June 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28537357/valedictory-editorial-the-past-six-years
#12
Michael A Lennon
Fifty years ago this month I graduated from the University of Liverpool. A few months earlier, along with three friends, I had driven overland to attend the International Dental Students Congress held that year in Israel. On the way we drove the spectacular road from Beirut up to Damascus, visited Petra in the Southern Jordanian desert, at that time devoid of tourists, viewed the Chagall windows in the synagogue on the Hadassah University Medical campus, and listened to the world cup final with a group of German students in a youth hostel overlooking the Mandelbaum Gate...
December 2016: Community Dental Health
https://www.readbyqxmd.com/read/28537348/a-cadaver-study-to-evaluate-the-accuracy-of-a-new-3d-mini-optical-navigation-tool-for-total-hip-arthroplasty
#13
Jonathan M Vigdorchik, Michael B Cross, Eric A Bogner, Theodore T Miller, Jeffrey M Muir, Ran Schwarzkopf
BACKGROUND: Accurate measurement of acetabular cup position (CP), changes in leg length (LL), and offset (OS) are paramount in ensuring proper sizing and implantation of components during total hip arthroplasty (THA). LL/OS inaccuracies can cause low back pain, neurological deficits, and patient dissatisfaction, while inaccurate positioning of the acetabular cup can lead to instability, dislocation, and, ultimately, revision surgery. The objective of this study was to evaluate the accuracy of a mini-navigation tool in measuring CP and LL/OS differential during THA...
May 24, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28537336/dietary-risk-factors-by-race-ethnicity-age-group-and-gender-in-a-representative-sample-of-us-older-adults
#14
J A Vaccaro, F G Huffman
OBJECTIVE: To explore the relationships among ethnicity/race, gender, demographics, age-group and dietary health in a nationally representative sample of older adults. DESIGN: Cross-sectional study. SETTING: Data for this study were collected by interview in the mobile examination centers from the National Health and Nutrition Examination Surveys, 2011 - 2012. PARTICIPANTS: U.S. representative sample of adults aged 55 years and older (N = 1860) from five ethnic/racial groups...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28537030/chemical-contamination-assessment-in-mangrove-lined-caribbean-coastal-systems-using-the-oyster-crassostrea-rhizophorae-as-biomonitor-species
#15
Javier R Aguirre-Rubí, Andrea Luna-Acosta, Nestor Etxebarría, Manu Soto, Félix Espinoza, Michael J Ahrens, Ionan Marigómez
This paper aims to contribute to the use of mangrove cupped oyster, Crassostrea rhizophorae, as a biomonitor species for chemical contamination assessment in mangrove-lined Caribbean coastal systems. Sampling was carried out in eight localities (three in Nicaragua and five in Colombia) with different types and levels of contamination. Oysters were collected during the rainy and dry seasons of 2012-2013 and the tissue concentrations of metals, polycyclic aromatic hydrocarbons (PAHs), and persistent organic pollutants (POPs) were determined...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28536701/endotracheal-intubation-using-a-direct-laryngoscope-and-the-protective-performances-of-respirators-a-randomized-trial
#16
Taeho Lim, Sanghyun Lee, Jaehoon Oh, Hyunggoo Kang, Chiwon Ahn, Yeongtak Song, Juncheol Lee, Hyungoo Shin
Purpose. Emergency physicians are at risk for infection during invasive procedures, and the respirators can reduce this risk. This study aimed to determine whether endotracheal intubation using direct laryngoscopes affected protection performances of respirators. Methods. A randomized crossover study of 24 emergency physicians was performed. We performed quantitative fit tests using respirators (cup type, fold type without a valve, and fold type with a valve) before and during intubation. The primary outcome was respirators' fit factors (FF), and secondary outcomes were acceptable protection (percentage of scores above 100 FF [FF%])...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28534225/biomarker-monitoring-of-controlled-dietary-acrylamide-exposure-indicates-consistent-human-endogenous-background
#17
Katharina Goempel, Laura Tedsen, Meike Ruenz, Tamara Bakuradze, Dorothea Schipp, Jens Galan, Gerhard Eisenbrand, Elke Richling
The aim of the present study was to explore the relation of controlled dietary acrylamide (AA) intake with the excretion of AA-related urinary mercapturic acids (MA), N-acetyl-S-(carbamoylethyl)-L-cysteine (AAMA) and N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA). Excretion kinetics of these short-term exposure biomarkers were monitored under strictly controlled conditions within a duplicate diet human intervention study. One study arm (group A, n = 6) ingested AA via coffee (0.15-0.17 µg/kg bw) on day 6 and in a meal containing an upper exposure level of AA (14...
May 22, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28532653/hippocampal-ca1-local-field-potential-oscillations-induced-by-olfactory-cue-of-liked-food
#18
Nifareeda Samerphob, Dania Cheaha, Surapong Chatpun, Ekkasit Kumarnsit
Eating motivation is induced not only by negative energy balance but also food related cues. However, neural processing for acquisition of learned food preference remains to be established. This study aimed to identify hippocampal neural signaling in response to olfactory cue (chocolate scent) after completion of repetitive chocolate sessions. Male Swiss albino mice implanted with intracranial electrode into the hippocampus were used for local field potential (LFP) recording. Animals were given chocolate sessions (a piece of 2 g chocolate per each mouse to eat on day 1, 3, 5 and 7)...
May 19, 2017: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/28532249/breast-volume-is-affected-by-body-mass-index-but-not-age
#19
Celeste E Coltman, Julie R Steele, Deirdre E McGhee
BACKGROUND: This study aimed to establish normative breast volume data for women of varying ages, body masses and breast sizes, and to determine the effect of age and body mass index (BMI) on breast volume. METHODS: The breast volume of 356 women (age range: 18.1-83.7 years; BMI range: 18.4-54.5 kg/m²) was measured using three-dimensional scanning in a prone position Results: Breast volumes ranged from 48 mL - 3100 mL. Although breast volume was not significantly affected by age, it was significantly affected by BMI, with the breast volume of overweight and obese women being two-to-three times greater than women with normal BMI's...
May 22, 2017: Ergonomics
https://www.readbyqxmd.com/read/28532076/sustained-drug-release-from-surface-modified-uhmwpe-for-acetabular-cup-lining-in-total-hip-implant
#20
R Manoj Kumar, Pallavi Gupta, Sandan Kumar Sharma, Akshat Mittal, Manish Shekhar, Vijayesh Kumar, B V Manoj Kumar, Partha Roy, Debrupa Lahiri
Despite sterilization and aseptic procedure, bacterial infection remains a key challenge in total hip arthroplasties. This fact emphasizes the urgent need for development of new implant systems, which should releases the drug in a controlled manner without sparing its mechanical and tribological properties. In this study, the lining material of the acetabular cup, in total hip implant, has been modified for sustained release of drugs, which should be available throughout the site of implantation to fight the post-operation bacterial infection...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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