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https://www.readbyqxmd.com/read/28446539/transient-mechanical-strain-promotes-the-maturation-of-invadopodia-and-enhances-cancer-cell-invasion-in-vitro
#1
Alexander N Gasparski, Snehal Ozarkar, Karen A Beningo
Cancer cell invasion is influenced by various biomechanical forces found within the microenvironment. We have previously found that invasion is enhanced in fibrosarcoma cells when transient mechanical stimulation is applied within an in vitro mechano-invasion assay. This enhancement of invasion is dependent on cofilin, a known regulator of invadopodia maturation. Invadopodia are actin-rich structures present in invasive cancer cells that are enzymatically active and degrade the surrounding extracellular matrix to facilitate invasion...
April 26, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28445157/lim-kinases-cofilin-and-beyond
#2
Chloé Prunier, Renaud Prudent, Reuben Kapur, Karin Sadoul, Laurence Lafanechère
LIM kinases are common downstream effectors of several signalization pathways and function as a signaling node that controls cytoskeleton dynamics through the phosphorylation of the cofilin family proteins. These last 10 years, several reports indicate that the functions of LIM kinases are more extended than initially described and, specifically, that LIM kinases also control microtubule dynamics, independently of their regulation of actin microfilament. In this review we analyze the data supporting these conclusions and the possible mechanisms that could be involved in the control of microtubules by LIM kinases...
April 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28440461/the-role-of-mir-451-in-the-switching-between-proliferation-and-migration-in-malignant-glioma-cells-ampk-signaling-mtor-modulation-and-rac1-activation-required
#3
Kai Zhao, Leilei Wang, Tao Li, Meng Zhu, Chen Zhang, Lei Chen, Pengfei Zhao, Hua Zhou, Shengping Yu, Xuejun Yang
Glioblastoma multiforme (GBM), WHO grade IV astrocytoma, is the most common primary neoplasm of the central nervous system (CNS) and has the highest malignancy and mortality rates. The invasive nature of GBM complicates surgical resection and restricts chemotherapeutic access, contributing to poor patient prognosis. The migration of tumor cells is closely related to the tumor cell proliferation. The acquisition of migratory capability, in addition to intracellular factors, is proposed to be a crucial mechanism during the progression of invasion...
April 21, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28440035/mir-145-inhibits-human-colorectal-cancer-cell-migration-and-invasion-via-pak4-dependent-pathway
#4
Nengquan Sheng, Gewen Tan, Weiqiang You, Hongqi Chen, Jianfeng Gong, Di Chen, Huizhen Zhang, Zhigang Wang
MicroRNA-145 (miR-145), as a tumor-suppressive miRNA, has been demonstrated down-regulated in colorectal cancer (CRC) cells, and could inhibit CRC cells growth. However, the molecular pathway in which miR-145 modulates CRC malignant transformation has not been fully revealed. Here, we reported an intense correlation between the expressions of PAK4 and miR-145 in human CRC cell lines. Transwell assay verified overexpression of miR-145, as well as knockdown of PAK4, significantly suppressed cell migration and invasion ability...
April 24, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28422872/effect-of-hsp27-and-cofilin-in-the-injury-of-hypoxia-reoxygenation-on-hepatocyte-membrane-f-actin-microfilaments
#5
Yafei Zhang, Jiazhong Wang, Hong Ji, Hongwei Lu, Le Lu, Jinlong Wang, Yiming Li
Hypoxia-reoxygenation (H/R) injury hepatocyte models were established to simulate the ischemia/reperfusion injury of transplanted organ. Through the study of the molecular mechanism of H/R on the F-actin damage of the liver cytomembrane, the mechanism of F-actin damage induced by ischemia and reperfusion was studied from the level of cell and molecule.The hypoxic environment of cells in vitro was simulated by chemical hypoxia agent CoCl2. Liver cells were detected by MTT, H/R group was subdivided into 3 subgroups: H/R 2, 4, and 6 h...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28416631/mutation-of-neuron-specific-chromatin-remodeling-subunit-baf53b-rescue-of-plasticity-and-memory-by-manipulating-actin-remodeling
#6
Annie Vogel Ciernia, Enikö A Kramár, Dina P Matheos, Robbert Havekes, Thekla J Hemstedt, Christophe N Magnan, Keith Sakata, Ashley Tran, Soraya Azzawi, Alberto Lopez, Richard Dang, Weisheng Wang, Brian Trieu, Joyce Tong, Ruth M Barrett, Rebecca J Post, Pierre Baldi, Ted Abel, Gary Lynch, Marcelo A Wood
Recent human exome-sequencing studies have implicated polymorphic Brg1-associated factor (BAF) complexes (mammalian SWI/SNF chromatin remodeling complexes) in several intellectual disabilities and cognitive disorders, including autism. However, it remains unclear how mutations in BAF complexes result in impaired cognitive function. Post-mitotic neurons express a neuron-specific assembly, nBAF, characterized by the neuron-specific subunit BAF53b. Subdomain 2 of BAF53b is essential for the differentiation of neuronal precursor cells into neurons...
May 2017: Learning & Memory
https://www.readbyqxmd.com/read/28409884/altered-protein-expression-pattern-in-colon-tissue-of-mice-upon-supplementation-with-distinct-selenium-compounds
#7
Jette Rahn, Claudia Lennicke, Anna P Kipp, Andreas S Müller, Ludger A Wessjohann, Rudolf Lichtenfels, Barbara Seliger
The essential trace element Selenium (Se) is controversially discussed concerning its role in health and disease. Its various physiological functions are largely mediated by Se incorporation in the catalytic center of selenoproteins. To gain insights into the impact of Se deficiency and of supplementation with different Se compounds (selenite, selenate, selenomethionine) at defined concentrations (recommended, 150 μg/ kg diet; excessive, 750 μg/ kg diet) in murine colon tissues a twenty week feeding experiment was performed followed by analysis of the protein expression pattern of colon tissue specimens by 2D-DIGE and MALDI-TOF MS...
April 14, 2017: Proteomics
https://www.readbyqxmd.com/read/28404907/investigation-of-three-potential-autoantibodies-in-sjogren-s-syndrome-and-associated-malt-lymphoma
#8
Li Cui, Naseim Elzakra, Shuaimei Xu, Gary Guishan Xiao, Yan Yang, Shen Hu
Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease which might progress to mucosal-associated lymphoid tissue lymphoma (pSS/MALT). Diagnosis of pSS requires an invasive tissue biopsy and a delay in diagnosis of pSS has been frequently reported. In this study, four proteins including cofilin-1, alpha-enolase, annexin A2 and Rho GDP-dissociation inhibitor 2 (RGI2) were found to be over-expressed in pSS and pSS/MALT by 2D gel electrophoresis/mass spectrometry, and the finding was verified by the microarray analysis and western blotting results...
February 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28388575/cofilin-1-induces-the-epithelial-mesenchymal-transition-of-gastric-cancer-cells-by-promoting-cytoskeletal-rearrangement
#9
Wang Haibo, Tao Lide, Jin Feng, Gu Hao, Dai Xiaojun, Ni Tengyang, Feng Jun, Ding Yanbing, Xiao Weiming, Qian Yayun, Liu Yanqing
Epithelial-mesenchymal transition (EMT) is an important biological process whereby malignant tumor cells obtain the ability to migrate, invade, resist apoptosis and degrade the extracellular matrix. We found that Cofilin1 (CFL1) expression was elevated in clinical gastric cancer specimens and correlated with biomarkers of EMT in BGC-823 gastric cancer cells. BGC-823 cells exhibited EMT phenotypes and increased metastatic ability when induced by TGF-β1. By contrast, BGC-823 cells transfected with Lv-siRNA-CFL1 did not exhibit EMT phenotypes under the same inducing conditions...
March 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28386357/fasudil-inhibits-proliferation-and-collagen-synthesis-and-induces-apoptosis-of-human-fibroblasts-derived-from-urethral-scar-via-the-rho-rock-signaling-pathway
#10
Ning Xu, Shao-Hao Chen, Gen-Yi Qu, Xiao-Dong Li, Wen Lin, Xue-Yi Xue, Yun-Zhi Lin, Qing-Shui Zheng, Yong Wei
Fasudil has shown antifibrotic effects in various fibrotic diseases. However, its effects on human urethral fibroblasts are unknown. This study evaluated the effects of fasudil on cellular proliferation, migration, apoptosis, and collagen synthesis in human fibroblasts derived from urethral scar tissues. Human urethral scar fibroblasts were cultured by explant and incubated for 24 h or 48 h with fasudil (12.5, 25, 50 µmol/L) with or without transforming growth factor β1 (TGF-β1, 10 ng/mL), or left untreated (control)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28385010/coactosin-like-protein-1-inhibits-the-neuronal-migration-during-mouse-corticogenesis
#11
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao
Coactosin-like protein-1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from the soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains unknown. Here, we revealed that Cotl1 overexpression impaired the migration of both early- and late-born neurons during mouse corticogenesis. Moreover, Cotl1 overexpression delayed, rather than blocked, neuronal migration in late-born neurons...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28381571/discovery-of-novel-small-molecule-inhibitors-of-lim-domain-kinase-for-inhibiting-hiv-1
#12
Fei Yi, Jia Guo, Deemah Dabbagh, Mark Spear, Sijia He, Kylene Kehn-Hall, Jacque Fontenot, Yan Yin, Mathieu Bibian, Chul Min Park, Ke Zheng, HaJeung Park, Veronica Soloveva, Dima Gharaibeh, Cary Retterer, Rouzbeh Zamani, Margaret L Pitt, John Naughton, Yongjun Jiang, Hong Shang, Ramin M Hakami, Binhua Ling, John A T Young, Sina Bavari, Xuehua Xu, Yangbo Feng, Yuntao Wu
A dynamic actin cytoskeleton is necessary for viral entry, intracellular migration, and virion release. For HIV-1 infection, during entry, the virus triggers early actin activity through hijacking chemokine coreceptor signaling which activates a host dependency factor cofilin and its kinase, the LIM domain kinase (LIMK). Although knockdown of human LIMK1 with shRNA inhibits HIV infection, no specific small molecule inhibitor of LIMK was available. Here we describe the design and discovery of novel classes of small molecule inhibitors of LIMK for inhibiting HIV infection...
April 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28365778/deficiency-of-cks1-leads-to-learning-and-long-term-memory-defects-and-p27-dependent-formation-of-neuronal-cofilin-aggregates
#13
Alexander Kukalev, Yiu-Ming Ng, Limei Ju, Amal Saidi, Sophie Lane, Angeles Mondragon, Dirk Dormann, Sophie E Walker, William Grey, Philip Wing-Lok Ho, David N Stephens, Antony M Carr, Karri Lamsa, Eric Tse, Veronica P C C Yu
In mitotic cells, the cyclin-dependent kinase (CDK) subunit protein CKS1 regulates S phase entry by mediating degradation of the CDK inhibitor p27. Although mature neurons lack mitotic CDKs, we found that CKS1 was actively expressed in post-mitotic neurons of the adult hippocampus. Interestingly, Cks1 knockout (Cks1-/-) mice exhibited poor long-term memory, and diminished maintenance of long-term potentiation in the hippocampal circuits. Furthermore, there was neuronal accumulation of cofilin-actin rods or cofilin aggregates, which are associated with defective dendritic spine maturation and synaptic loss...
January 1, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28363850/di-n-butyl-phthalate-exposure-negatively-influences-structural-and-functional-neuroplasticity-via-rho-gtpase-signaling-pathways
#14
Yuemin Ding, Lingchao Lu, Chengkai Xuan, Jiajv Han, Shumin Ye, Tingting Cao, Weibo Chen, Aiqing Li, Xiong Zhang
Di-n-butyl phthalate (DBP) has been reported to cause disruptions in hippocampal plasticity, but its specific mechanism has not yet been ascertained. In this research, a mouse model of chronic DBP exposure was generated by intragastric administration of DBP (10, 50, or 250°mg/kg/d) for 5 weeks. Chronic exposure to high concentrations of DBP (250°mg/kg/d) induced a spatial learning deficit in the Morris water maze in male mice. By determining the activity of Rho-GTPase signaling pathways in the hippocampal tissues, we found that DBP exposure inhibited the activity of Rac1/PAK1/LIMK1 but activated RhoA/ROCK/LIMK2 signaling and eventually suppressed cofilin activity by phosphorylation...
March 28, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28359791/plumbagin-reduces-osteopontin-induced-invasion-through-inhibiting-the-rho-associated-kinase-signaling-pathway-in-a549-cells-and-suppresses-osteopontin-induced-lung-metastasis-in-balb-c-mice
#15
Chi Gu Kang, Eunji Im, Hyo-Jeong Lee, Eun-Ok Lee
Lung cancer is the second most commonly diagnosed cancer and the leading cause of cancer deaths in both men and women in the United States. It has been recently demonstrated that osteopontin (OPN) effectively inhibits cofilin activity through the focal adhesion kinase (FAK)/AKT/Rho-associated kinase (ROCK) pathway to induce the invasion of human non-small cell lung cancer (NSCLC) cells. Plumbagin was isolated from the roots of the medicinal plant Plumbago zeylanica L. and has been reported to possess anticancer activities...
May 1, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28358024/downregulation-of-limk1-adf-cofilin-by-dads-inhibits-the-migration-and-invasion-of-colon-cancer
#16
Jian Su, Yujuan Zhou, Zhibing Pan, Ling Shi, Jing Yang, Aijun Liao, Qianjin Liao, Qi Su
This study aimed to explore whether the downregulation of LIM kinase 1 (LIMK1)-actin depolymerization factor (ADF, also known as destrin)/cofilin by diallyl disulfide (DADS) inhibited the migration and invasion of colon cancer. Previous studies have shown that silencing LIMK1 could significantly enhance the inhibitory effect of DADS on colon cancer cell migration and invasion, suggesting that LIMK1 was a target molecule of DADS, which needed further confirmation. This study reported that LIMK1 and destrin were highly expressed in colon cancer and associated with poor prognosis of patients with colon cancer...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303963/structural-analysis-of-human-cofilin-2-filamentous-actin-assemblies-atomic-resolution-insights-from-magic-angle-spinning-nmr-spectroscopy
#17
Jenna Yehl, Elena Kudryashova, Emil Reisler, Dmitri Kudryashov, Tatyana Polenova
Cellular actin dynamics is an essential element of numerous cellular processes, such as cell motility, cell division and endocytosis. Actin's involvement in these processes is mediated by many actin-binding proteins, among which the cofilin family plays unique and essential role in accelerating actin treadmilling in filamentous actin (F-actin) in a nucleotide-state dependent manner. Cofilin preferentially interacts with older filaments by recognizing time-dependent changes in F-actin structure associated with the hydrolysis of ATP and release of inorganic phosphate (Pi) from the nucleotide cleft of actin...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300846/regulation-of-rhoa-activity-by-the-cellular-prion-protein
#18
Hee-Jun Kim, Hong-Seok Choi, Jeong-Ho Park, Mo-Jong Kim, Hyoung-Gon Lee, Robert Bob Petersen, Yong-Sun Kim, Jae-Bong Park, Eun-Kyoung Choi
The cellular prion protein (PrP(C)) is a highly conserved glycosylphosphatidylinositol (GPI)-anchored membrane protein that is involved in the signal transduction during the initial phase of neurite outgrowth. The Ras homolog gene family member A (RhoA) is a small GTPase that is known to have an essential role in regulating the development, differentiation, survival, and death of neurons in the central nervous system. Although recent studies have shown the dysregulation of RhoA in a variety of neurodegenerative diseases, the role of RhoA in prion pathogenesis remains unclear...
March 16, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28293874/diversity-of-actin-architecture-in-human-osteoclasts-network-of-curved-and-branched-actin-supporting-cell-shape-and-intercellular-micrometer-level-tubes
#19
Paula Pennanen, Maria Helena Alanne, Elnaz Fazeli, Takahiro Deguchi, Tuomas Näreoja, Sirkku Peltonen, Juha Peltonen
Osteoclasts are multinucleated bone-resorbing cells with a dynamic actin cytoskeleton. Osteoclasts are derived from circulating mononuclear precursors. Confocal and stimulated emission depletion (STED) super-resolution microscopy was used to investigate peripheral blood-derived human osteoclasts cultured on glass surfaces. STED and confocal microscopy demonstrated that the actin was curved and branched, for instance, in the vicinity of membrane ruffles. The overall architecture of the curved actin network extended from the podosomes to the top of the cell...
March 14, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28282023/competition-between-tropomyosin-fimbrin-and-adf-cofilin-drives-their-sorting-to-distinct-actin-filament-networks
#20
Jenna R Christensen, Glen M Hocky, Kaitlin E Homa, Alisha N Morganthaler, Sarah E Hitchcock-DeGregori, Gregory A Voth, David R Kovar
The fission yeast actin cytoskeleton is an ideal, simplified system to investigate fundamental mechanisms behind cellular self-organization. By focusing on the stabilizing protein tropomyosin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pairwise and collective interactions with actin filaments regulate their activity and segregation to functionally diverse F-actin networks. Utilizing multi-color TIRF microscopy of in vitro reconstituted F-actin networks, we observed and characterized two distinct Cdc8 cables loading and spreading cooperatively on individual actin filaments...
March 10, 2017: ELife
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