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https://www.readbyqxmd.com/read/29166118/photofragmentation-of-gas-phase-acetic-acid-and-acetamide-clusters-in-the-vacuum-ultraviolet-region
#1
Marta Berholts, Hanna Myllynen, Kuno Kooser, Eero Itälä, Sari Granroth, Helena Levola, Joakim Laksman, Shabnam Oghbaiee, Bart Oostenrijk, Ergo Nõmmiste, Edwin Kukk
Photofragmentation of gas-phase acetamide and acetic acid clusters produced by a supersonic expansion source has been studied using time-of-flight mass spectrometry and the partial ion yield (PIY) technique combined with tunable vacuum-ultraviolet synchrotron radiation. Appearance energies of the clusters and their fragments were experimentally determined from the PIY measurements. The effect of clusterization conditions on the formation and fragmentation of acetic acid clusters was investigated. Ab initio quantum mechanical calculations were performed on both samples' dimers to find their neutral and ionized geometries as well as proton transfer energy barriers leading to the optimal geometries...
November 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29117187/the-green-tea-modulates-large-intestinal-microbiome-and-exo-endogenous-metabolome-altered-through-chronic-uvb-exposure
#2
Eun Sung Jung, Hye Min Park, Seung Min Hyun, Jong Cheol Shon, Digar Singh, Kwang-Hyeon Liu, Tae Woong Whon, Jin-Woo Bae, Jae Sung Hwang, Choong Hwan Lee
The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-exposed mice groups (UVB), class Bacilli and order Bifidobacteriales were observed as discriminant taxa with decreased lysophospholipid levels compared to the unexposed mice groups subjected to normal diet (NOR)...
2017: PloS One
https://www.readbyqxmd.com/read/29114650/electrochemical-reactive-oxygen-species-detection-by-cytochrome-c-immobilized-with-vertically-aligned-and-electrochemically-reduced-graphene-oxide-on-a-glassy-carbon-electrode
#3
Dinakaran Thirumalai, Vijayaraj Kathiresan, Jaewon Lee, Sung-Ho Jin, Seung-Cheol Chang
A new amperometric biosensor for hydrogen peroxide (H2O2) and superoxide anion (SO) has been developed. The biosensor developed uses cytochrome c (Cyt c) modified glassy carbon electrodes coupled with electrochemically reduced graphene oxide (ErGO). To immobilize Cyt c, the "one step" electrochemical deposition of vertically aligned and ErGO (VAErGO) has been performed by using a pulse reverse technique, thus resulting in a very simple and efficient system. The well-established vertical alignment of ErGO was confirmed by atomic force microscopy and the electrochemical characteristics of the biosensor were investigated by cyclic voltammetric, electrochemical impedance spectroscopy and amperometric techniques...
November 8, 2017: Analyst
https://www.readbyqxmd.com/read/29112055/self-selected-resistance-exercise-load-implications-for-research-and-prescription
#4
Sebastião Barbosa-Netto, Obanshe S dʼAcelino-E-Porto, Marcos B Almeida
Barbosa-Netto, S, d'Acelino-e-Porto, OS, and Almeida, MB. Self-selected resistance exercise load: implications for research and prescription. J Strength Cond Res XX(X): 000-000, 2017-Resistance training has been widely used for maintaining or improving the quality of life and sports performance. It is not clear, however, whether the load commonly used in these exercises is equivalent to the number of repetitions determined for execution, that is, the level of effort applied to the self-selected load. Ergo, the aim of this study was to identify the number of maximum repetitions that strength training practitioners can perform with the load commonly used (self-selected) to perform 10 repetitions in their training routines...
November 1, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29055194/a-graphene-quantum-dots-based-electrochemiluminescence-immunosensor-for-carcinoembryonic-antigen-detection-using-poly-5-formylindole-reduced-graphene-oxide-nanocomposite
#5
Guangming Nie, Yang Wang, Yun Tang, Dan Zhao, Qingfu Guo
A novel electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of carcinoembryonic antigen (CEA) was developed using signal amplification strategy based on poly(5-formylindole)/reduced graphene oxide nanocomposite (P5FIn/erGO) and Au nanoparticle (AuNP) decorated graphene quantum dots (GQDs) (GQDs@AuNP). As an effective matrix for immobilization of primary antibody (Ab1), P5FIn/erGO nanocomposite facilitated the ion transport during the redox reactions and provided larger surface areas for the immobilization of Ab1...
October 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28988355/updating-the-evidence-on-functional-capacity-evaluation-methods-a-systematic-review
#6
REVIEW
Stijn De Baets, Patrick Calders, Noortje Schalley, Katrien Vermeulen, Sofie Vertriest, Lien Van Peteghem, Marieke Coussens, Fransiska Malfait, Guy Vanderstraeten, Geert Van Hove, Dominique Van de Velde
Objectives To synthesize the evidence on the psychometrics functional capacity evaluation (FCE) methods. Methods A systematic literature search in nine databases. The resulting articles were screened based on predefined in- and exclusion criteria. Two reviewers independently performed this screening. Included studies were appraised based on their methodological quality. Results The search resulted in 20 eligible studies about nine different FCE methods. The Baltimore Therapeutic Equipment work simulator showed a moderate predictive validity...
October 7, 2017: Journal of Occupational Rehabilitation
https://www.readbyqxmd.com/read/28953071/applying-machine-learning-to-workers-compensation-data-to-identify-industry-specific-ergonomic-and-safety-prevention-priorities-ohio-2001-to-2011
#7
Alysha R Meyers, Ibraheem S Al-Tarawneh, Steven J Wurzelbacher, P Timothy Bushnell, Michael P Lampl, Jennifer L Bell, Stephen J Bertke, David C Robins, Chih-Yu Tseng, Chia Wei, Jill A Raudabaugh, Teresa M Schnorr
OBJECTIVE: This study leveraged a state workers' compensation claims database and machine learning techniques to target prevention efforts by injury causation and industry. METHODS: Injury causation auto-coding methods were developed to code more than 1.2 million Ohio Bureau of Workers' Compensation claims for this study. Industry groups were ranked for soft-tissue musculoskeletal claims that may have been preventable with biomechanical ergonomic (ERGO) or slip/trip/fall (STF) interventions...
September 25, 2017: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28842007/highly-sensitive-amperometric-biosensor-for-determination-of-nadh-and-ethanol-based-on-au-ag-nanoparticles-poly-l-cysteine-reduced-graphene-oxide-nanocomposite
#8
Gözde Aydoğdu Tığ
This work presents the fabrication of a novel nicotinamide adenine dinucleotide (NADH) sensor using gold-silver bimetallic nanoparticles (Au-AgNPs), poly(L-Cysteine) (P(L-Cys)) and electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE/Au-AgNPs/P(L-Cys)-ERGO). The composite electrode exhibited an excellent electrocatalytic response towards NADH at a low oxidation potential (+ 0.35V) and minimization of surface contamination due to the synergistic effects of the Au-AgNPs, polymer and ERGO...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28819581/electrochemical-evaluation-of-trans-resveratrol-levels-in-red-wine-based-on-the-interaction-between-resveratrol-and-graphene
#9
Lantao Liu, Yanli Zhou, Yiyu Kang, Haihong Huang, Congming Li, Maotian Xu, Baoxian Ye
trans-Resveratrol is often considered as one of the quality standards of red wine, and the development of a sensitive and reliable method for monitoring the trans-resveratrol levels in red wine is an urgent requirement for the quality control. Here, a novel voltammetric approach was described for probing trans-resveratrol using a graphene-modified glassy carbon (GC) electrode. The proposed electrode was prepared by one-step electrodeposition of reduced graphene oxide (ERGO) at a GC electrode. Compared with the bare GC electrode, the introduced graphene film on the electrode surface dramatically improved the sensitivity of the sensor response due to the π-π interaction between the graphene and trans-resveratrol...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28768995/physiological-roles-of-carnitine-organic-cation-transporter-octn1-slc22a4-in-neural-cells
#10
Noritaka Nakamichi, Yukio Kato
Dysfunction in neurotransmission mediated by neurotransmitters causes various neurological disorders. Therefore, receptors and reuptake transporters of neurotransmitters have been focused on as a therapeutic target in neurological disorders. These membrane proteins have high affinity for a specific neurotransmitter and are highly expressed on synaptic membranes. In contrast, xenobiotic transporters have relatively lower affinity for neurotransmitters but widely recognize various organic cations and/or anions and are also expressed in brain neurons...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28762596/effectiveness-of-home-based-exercise-in-older-patients-with-advanced-chronic-obstructive-pulmonary-disease-a-3-year-cohort-study
#11
Ritsuko Wakabayashi, Yuji Kusunoki, Kumiko Hattori, Takashi Motegi, Ryuko Furutate, Aki Itoh, Rupert Cm Jones, Michael E Hyland, Kozui Kida
AIM: To determine whether home-based exercise can improve clinical outcomes in older patients with advanced chronic obstructive pulmonary disease using long-term oxygen therapy. METHODS: Information was provided to improve chronic obstructive pulmonary disease self-management before the onset of the present prospective 3-year cohort study. Patients selected either home-based exercise using a lower-limb cycle machine (ergo-bicycle; group E), or usual exercise (group U)...
August 1, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/28746901/enhanced-electrocatalytic-activity-of-graphene-gold-nanoparticles-hybrids-for-peroxynitrite-electrochemical-detection-on-hemin-based-electrode
#12
Beibei Wang, Xueping Ji, Jujie Ren, Ruixing Ni, Lin Wang
A simple, ultrasensitive peroxynitrite anion (ONOO(-)) electrochemical sensing platform was developed by immobilizing hemin on a density controllable electrochemically reduced graphene oxide-Au nanoparticles (ERGO-AuNPs) nanohybrids. The ERGO-AuNPs in situ nanohybrids were produced onto a glass carbon electrode (GCE) by one-step electrodeposition, the density of which could be easily controlled by electrodeposited time. The morphology of ERGO-AuNPs nanohybrids was characterized by a scanning electron microscope (SEM)...
December 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28709466/effects-of-inhaled-hypertonic-7-saline-on-lung-function-test-in-preschool-children-with-cystic-fibrosis-results-of-a-crossover-randomized-clinical-trial
#13
Raffaella Nenna, Fabio Midulla, Caterina Lambiase, Giovanna De Castro, Anna Maria Zicari, Luciana Indinnimeo, Giuseppe Cimino, Patrizia Troiani, Serena Quattrucci, Giancarlo Tancredi
BACKGROUND: This crossover, randomized, double-blind study (conducted over a 32-week period) was performed to determine, in clinically stable Cystic fibrosis (CF) preschool children: the effects of 7% inhaled hypertonic saline on spirometry and interrupter resistance technique (Rint), and the possible side effects. METHODS: Twelve CF children (6M, mean age ± SD: 5.7 ± 0.8 yrs) were enrolled and randomly assigned to receive hypertonic saline (HS-4 ml 7% sodium chloride), or normal saline (NS-0...
July 15, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28688036/carnitine-organic-cation-transporter-octn1-negatively-regulates-activation-in-murine-cultured-microglial-cells
#14
Takahiro Ishimoto, Noritaka Nakamichi, Hikari Nishijima, Yusuke Masuo, Yukio Kato
Brain immune cells, i.e., microglia, play an important role in the maintenance of brain homeostasis, whereas chronic overactivation of microglia is involved in the development of various neurodegenerative disorders. Therefore, the regulation of microglial activation may contribute to their treatment. The aim of the present study was to clarify the functional expression of carnitine/organic cation transporter OCTN1/SLC22A4, which recognizes the naturally occurring food-derived antioxidant ergothioneine (ERGO) as a substrate in vivo, in microglia and its role in regulation of microglial activation...
July 8, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28677654/reduced-graphene-oxides-influence-of-the-reduction-method-on-the-electrocatalytic-effect-towards-nucleic-acid-oxidation
#15
Daniela F Báez, Helena Pardo, Ignacio Laborda, José F Marco, Claudia Yáñez, Soledad Bollo
For the first time a critical analysis of the influence that four different graphene oxide reduction methods have on the electrochemical properties of the resulting reduced graphene oxides (RGOs) is reported. Starting from the same graphene oxide, chemical (CRGO), hydrothermal (hTRGO), electrochemical (ERGO), and thermal (TRGO) reduced graphene oxide were produced. The materials were fully characterized and the topography and electroactivity of the resulting glassy carbon modified electrodes were also evaluated...
July 4, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28650069/transcriptional-engineering-of-the-glyceraldehyde-3-phosphate-dehydrogenase-promoter-for-improved-heterologous-protein-production-in-pichia-pastoris
#16
Özge Ata, Roland Prielhofer, Brigitte Gasser, Diethard Mattanovich, Pınar Çalık
The constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP ), which is one of the benchmark promoters of Pichia pastoris, was analyzed in terms of putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication of these putative transcription factor binding sites and selected transcription factor (TF) genes were overexpressed or deleted to understand their roles on heterologous protein production. Using enhanced green fluorescent protein, an expression strength in a range between 0...
October 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28622945/safety-of-intracoronary-provocative-testing-for-the-diagnosis-of-coronary-artery-spasm
#17
Giuseppe Ciliberti, Sreenivasa Rao Kondapally Seshasai, Giuseppe Ambrosio, Juan Carlos Kaski
AIMS: Systematic review of literature to evaluate safety of intracoronary (i.c.) pharmacologic testing with acetylcholine (ACh), or ergonovine (ERGO), to induce coronary artery spasm. METHODS AND RESULTS: Review of all relevant publications using MEDLINE and EMBASE databases yielded 10 publications, totalling 9,444 patients. Prevalence of provoked spasm varied from 2.3% to 54.7% of patients tested in the selected studies. The wide variability in prevalence was due to heterogeneity of study populations and provocation protocols...
October 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28533890/effect-of-music-tempo-on-exercise-performance-and-heart-rate-among-young-adults
#18
Avinash E Thakare, Ranjeeta Mehrotra, Ayushi Singh
BACKGROUND & OBJECTIVES: Music captures attention, triggers a range of emotions, alters or regulates mood, increases work output, heightens arousal, induces states of higher functioning, reduces inhibitions and encourages rhythmic movement. Music has ergo-genic effect as well, it increases exercise performance, delays fatigue and increases performance and endurance, power and strength. Our study tried to evaluate the effect of music on exercise performance in young untrained subjects...
2017: International Journal of Physiology, Pathophysiology and Pharmacology
https://www.readbyqxmd.com/read/28532045/electrochemically-reduced-graphene-oxide-poly-glycine-composite-modified-electrode-for-sensitive-determination-of-l-dopa
#19
Venkata Narayana Palakollu, Neeta Thapliyal, Tirivashe E Chiwunze, Rajshekhar Karpoormath, Sivanandhan Karunanidhi, Srinivasulu Cherukupalli
A facile preparation strategy based on electrochemical technique for the fabrication of glycine (Poly-Gly) and electrochemically reduced graphene oxide (ERGO) composite modified electrode was developed. The morphology of the developed composite (ERGO/Poly-Gly) was investigated using field emission scanning electron microscope (FE-SEM). The composite modified glassy carbon electrode (GCE) was characterized using fourier transform-infrared (FT-IR) spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28472750/effect-of-the-presence-of-cholesterol-in-the-interfacial-microenvironment-on-the-modulation-of-the-alkaline-phosphatase-activity-during-in-vitro-mineralization
#20
B F Favarin, M A R Andrade, M Bolean, A M S Simão, A P Ramos, M F Hoylaerts, J L Millán, P Ciancaglini
Mineralization of the skeleton starts within cell-derived matrix vesicles (MVs); then, minerals propagate to the extracellular collagenous matrix. Tissue-nonspecific alkaline phosphatase (TNAP) degrades inorganic pyrophosphate (PPi), a potent inhibitor of mineralization, and contributes Pi (Phosphate) from ATP to initiate mineralization. Compared to the plasma membrane, MVs are rich in Cholesterol (Chol) (∼32%) and TNAP, but how Chol influences TNAP activity remains unclear. We have reconstituted TNAP in liposomes of dipalmitoylphosphatidylcholine (DPPC) or dioleoylphosphatidylcholine (DOPC) combined with Chol or its derivatives Cholestenone (Achol) and Ergosterol (Ergo)...
April 26, 2017: Colloids and Surfaces. B, Biointerfaces
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