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https://www.readbyqxmd.com/read/28819581/electrochemical-evaluation-of-trans-resveratrol-levels-in-red-wine-based-on-the-interaction-between-resveratrol-and-graphene
#1
Lantao Liu, Yanli Zhou, Yiyu Kang, Haihong Huang, Congming Li, Maotian Xu, Baoxian Ye
trans-Resveratrol is often considered as one of the quality standards of red wine, and the development of a sensitive and reliable method for monitoring the trans-resveratrol levels in red wine is an urgent requirement for the quality control. Here, a novel voltammetric approach was described for probing trans-resveratrol using a graphene-modified glassy carbon (GC) electrode. The proposed electrode was prepared by one-step electrodeposition of reduced graphene oxide (ERGO) at a GC electrode. Compared with the bare GC electrode, the introduced graphene film on the electrode surface dramatically improved the sensitivity of the sensor response due to the π-π interaction between the graphene and trans-resveratrol...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28768995/physiological-roles-of-carnitine-organic-cation-transporter-octn1-slc22a4-in-neural-cells
#2
Noritaka Nakamichi, Yukio Kato
Dysfunction in neurotransmission mediated by neurotransmitters causes various neurological disorders. Therefore, receptors and reuptake transporters of neurotransmitters have been focused on as a therapeutic target in neurological disorders. These membrane proteins have high affinity for a specific neurotransmitter and are highly expressed on synaptic membranes. In contrast, xenobiotic transporters have relatively lower affinity for neurotransmitters but widely recognize various organic cations and/or anions and are also expressed in brain neurons...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28762596/effectiveness-of-home-based-exercise-in-older-patients-with-advanced-chronic-obstructive-pulmonary-disease-a-3-year-cohort-study
#3
Ritsuko Wakabayashi, Yuji Kusunoki, Kumiko Hattori, Takashi Motegi, Ryuko Furutate, Aki Itoh, Rupert Cm Jones, Michael E Hyland, Kozui Kida
AIM: To determine whether home-based exercise can improve clinical outcomes in older patients with advanced chronic obstructive pulmonary disease using long-term oxygen therapy. METHODS: Information was provided to improve chronic obstructive pulmonary disease self-management before the onset of the present prospective 3-year cohort study. Patients selected either home-based exercise using a lower-limb cycle machine (ergo-bicycle; group E), or usual exercise (group U)...
August 1, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/28746901/enhanced-electrocatalytic-activity-of-graphene-gold-nanoparticles-hybrids-for-peroxynitrite-electrochemical-detection-on-hemin-based-electrode
#4
Beibei Wang, Xueping Ji, Jujie Ren, Ruixing Ni, Lin Wang
A simple, ultrasensitive peroxynitrite anion (ONOO(-)) electrochemical sensing platform was developed by immobilizing hemin on a density controllable electrochemically reduced graphene oxide-Au nanoparticles (ERGO-AuNPs) nanohybrids. The ERGO-AuNPs in situ nanohybrids were produced onto a glass carbon electrode (GCE) by one-step electrodeposition, the density of which could be easily controlled by electrodeposited time. The morphology of ERGO-AuNPs nanohybrids was characterized by a scanning electron microscope (SEM)...
July 15, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28709466/effects-of-inhaled-hypertonic-7-saline-on-lung-function-test-in-preschool-children-with-cystic-fibrosis-results-of-a-crossover-randomized-clinical-trial
#5
Raffaella Nenna, Fabio Midulla, Caterina Lambiase, Giovanna De Castro, Anna Maria Zicari, Luciana Indinnimeo, Giuseppe Cimino, Patrizia Troiani, Serena Quattrucci, Giancarlo Tancredi
BACKGROUND: This crossover, randomized, double-blind study (conducted over a 32-week period) was performed to determine, in clinically stable Cystic fibrosis (CF) preschool children: the effects of 7% inhaled hypertonic saline on spirometry and interrupter resistance technique (Rint), and the possible side effects. METHODS: Twelve CF children (6M, mean age ± SD: 5.7 ± 0.8 yrs) were enrolled and randomly assigned to receive hypertonic saline (HS-4 ml 7% sodium chloride), or normal saline (NS-0...
July 15, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28688036/carnitine-organic-cation-transporter-octn1-negatively-regulates-activation-in-murine-cultured-microglial-cells
#6
Takahiro Ishimoto, Noritaka Nakamichi, Hikari Nishijima, Yusuke Masuo, Yukio Kato
Brain immune cells, i.e., microglia, play an important role in the maintenance of brain homeostasis, whereas chronic overactivation of microglia is involved in the development of various neurodegenerative disorders. Therefore, the regulation of microglial activation may contribute to their treatment. The aim of the present study was to clarify the functional expression of carnitine/organic cation transporter OCTN1/SLC22A4, which recognizes the naturally occurring food-derived antioxidant ergothioneine (ERGO) as a substrate in vivo, in microglia and its role in regulation of microglial activation...
July 8, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28677654/reduced-graphene-oxides-influence-of-the-reduction-method-on-the-electrocatalytic-effect-towards-nucleic-acid-oxidation
#7
Daniela F Báez, Helena Pardo, Ignacio Laborda, José F Marco, Claudia Yáñez, Soledad Bollo
For the first time a critical analysis of the influence that four different graphene oxide reduction methods have on the electrochemical properties of the resulting reduced graphene oxides (RGOs) is reported. Starting from the same graphene oxide, chemical (CRGO), hydrothermal (hTRGO), electrochemical (ERGO), and thermal (TRGO) reduced graphene oxide were produced. The materials were fully characterized and the topography and electroactivity of the resulting glassy carbon modified electrodes were also evaluated...
July 4, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28650069/transcriptional-engineering-of-the-glyceraldehyde-3-phosphate-dehydrogenase-promoter-for-improved-heterologous-protein-production-in-pichia-pastoris
#8
Özge Ata, Roland Prielhofer, Brigitte Gasser, Diethard Mattanovich, Pınar Çalık
The constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP ), which is one of the benchmark promoters of Pichia pastoris, was analyzed in terms of putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication of these putative transcription factor binding sites and selected transcription factor (TF) genes were overexpressed or deleted to understand their roles on heterologous protein production. Using enhanced green fluorescent protein, an expression strength in a range between 0...
June 26, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28622945/safety-of-intracoronary-provocative-testing-for-the-diagnosis-of-coronary-artery-spasm
#9
Giuseppe Ciliberti, Sreenivasa Rao Kondapally Seshasai, Giuseppe Ambrosio, Juan Carlos Kaski
AIMS: Systematic review of literature to evaluate safety of intracoronary (i.c.) pharmacologic testing with acetylcholine (ACh), or ergonovine (ERGO), to induce coronary artery spasm. METHODS AND RESULTS: Review of all relevant publications using MEDLINE and EMBASE databases yielded 10 publications, totalling 9,444 patients. Prevalence of provoked spasm varied from 2.3% to 54.7% of patients tested in the selected studies. The wide variability in prevalence was due to heterogeneity of study populations and provocation protocols...
June 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28533890/effect-of-music-tempo-on-exercise-performance-and-heart-rate-among-young-adults
#10
Avinash E Thakare, Ranjeeta Mehrotra, Ayushi Singh
BACKGROUND & OBJECTIVES: Music captures attention, triggers a range of emotions, alters or regulates mood, increases work output, heightens arousal, induces states of higher functioning, reduces inhibitions and encourages rhythmic movement. Music has ergo-genic effect as well, it increases exercise performance, delays fatigue and increases performance and endurance, power and strength. Our study tried to evaluate the effect of music on exercise performance in young untrained subjects...
2017: International Journal of Physiology, Pathophysiology and Pharmacology
https://www.readbyqxmd.com/read/28532045/electrochemically-reduced-graphene-oxide-poly-glycine-composite-modified-electrode-for-sensitive-determination-of-l-dopa
#11
Venkata Narayana Palakollu, Neeta Thapliyal, Tirivashe E Chiwunze, Rajshekhar Karpoormath, Sivanandhan Karunanidhi, Srinivasulu Cherukupalli
A facile preparation strategy based on electrochemical technique for the fabrication of glycine (Poly-Gly) and electrochemically reduced graphene oxide (ERGO) composite modified electrode was developed. The morphology of the developed composite (ERGO/Poly-Gly) was investigated using field emission scanning electron microscope (FE-SEM). The composite modified glassy carbon electrode (GCE) was characterized using fourier transform-infrared (FT-IR) spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28472750/effect-of-the-presence-of-cholesterol-in-the-interfacial-microenvironment-on-the-modulation-of-the-alkaline-phosphatase-activity-during-in-vitro-mineralization
#12
B F Favarin, M A R Andrade, M Bolean, A M S Simão, A P Ramos, M F Hoylaerts, J L Millán, P Ciancaglini
Mineralization of the skeleton starts within cell-derived matrix vesicles (MVs); then, minerals propagate to the extracellular collagenous matrix. Tissue-nonspecific alkaline phosphatase (TNAP) degrades inorganic pyrophosphate (PPi), a potent inhibitor of mineralization, and contributes Pi (Phosphate) from ATP to initiate mineralization. Compared to the plasma membrane, MVs are rich in Cholesterol (Chol) (∼32%) and TNAP, but how Chol influences TNAP activity remains unclear. We have reconstituted TNAP in liposomes of dipalmitoylphosphatidylcholine (DPPC) or dioleoylphosphatidylcholine (DOPC) combined with Chol or its derivatives Cholestenone (Achol) and Ergosterol (Ergo)...
April 26, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28460072/exploring-the-structure-of-fucosylated-chondroitin-sulfate-through-bottom-up-nuclear-magnetic-resonance-and-electrospray-ionization-high-resolution-mass-spectrometry-approaches
#13
Gustavo Rc Santos, Ana Co Porto, Paulo Ag Soares, Eduardo Vilanova, Paulo As Mourão
Fucosylated chondroitin sulfate (FCS) from sea cucumbers is composed of a chondroitin sulfate (CS) central core and branches of sulfated fucose. The structure of this complex glycosaminoglycan is usually investigated via nuclear magnetic resonance (NMR) analyses of the intact molecule, ergo through a top-down approach, which often yield spectra with intricate sets of signals. Here we employed a bottom-up approach to analyze the FCSs from the sea cucumbers Isostichopus badionotus and Ludwigothurea grisea from their basic constituents, viz...
July 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28433808/comparisons-of-hip-strength-and-countermovement-jump-height-in-elite-tennis-players-with-and-without-acute-history-of-groin-injuries
#14
V Moreno-Pérez, A Lopez-Valenciano, D Barbado, J Moreside, J L L Elvira, F J Vera-Garcia
BACKGROUND: Despite the high groin-injury (GI) prevalence in tennis, no studies have assessed the extent to which intrinsic groin injury risk factors, such as hip muscle strength, have recovered in elite tennis players with a history of previous GI. OBJECTIVE: To investigate whether elite tennis players with a history of GI show differences in hip strength and jump height between injured and uninjured limbs and compared with dominant limb in tennis players without history of acute groin-injuries (NGI)...
June 2017: Musculoskeletal Science & Practice
https://www.readbyqxmd.com/read/28425050/oxidative-protein-modification-alters-proteostasis-under-acute-hypobaric-hypoxia-in-skeletal-muscles-a-comprehensive-in-vivo-study
#15
Akanksha Agrawal, Richa Rathor, Geetha Suryakumar
While numerous maladies are associated with hypobaric hypoxia, muscle protein loss is an important under studied topic. Hence, the present study was designed to investigate the mechanism of muscle protein loss at HH. SD rats were divided into normoxic rats, while remaining rats were exposed to simulated hypoxia equivalent to 282-torr pressure (equal to an altitude of 7620 m, 8% oxygen), at 25 °C for 6, 12, and 24 h. Post-exposure rats were sacrificed and analysis was performed. Ergo, muscle loss-related changes were observed at 12 and 24 h post-HH exposure...
May 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28366086/post-hoc-ergo-propter-hoc
#16
E Jane Costello
No abstract text is available yet for this article.
April 1, 2017: American Journal of Psychiatry
https://www.readbyqxmd.com/read/28338464/-doceo-ergo-sum-le-mentorat-des-chirurgiens
#17
Vivian McAlister
No abstract text is available yet for this article.
April 2017: Canadian Journal of Surgery. Journal Canadien de Chirurgie
https://www.readbyqxmd.com/read/28338463/doceo-ergo-sum-mentoring-surgeons
#18
Vivian McAlister
No abstract text is available yet for this article.
April 2017: Canadian Journal of Surgery. Journal Canadien de Chirurgie
https://www.readbyqxmd.com/read/28296182/endothelial-progenitors-a-consensus-statement-on-nomenclature
#19
Reinhold J Medina, Chad L Barber, Florence Sabatier, Francoise Dignat-George, Juan M Melero-Martin, Kiarash Khosrotehrani, Osamu Ohneda, Anna M Randi, Jerry K Y Chan, Teruhide Yamaguchi, Victor W M Van Hinsbergh, Mervin C Yoder, Alan W Stitt
Endothelial progenitor cell (EPC) nomenclature remains ambiguous and there is a general lack of concordance in the stem cell field with many distinct cell subtypes continually grouped under the term "EPC." It would be highly advantageous to agree on standards to confirm an endothelial progenitor phenotype and this should include detailed immunophenotyping, potency assays, and clear separation from hematopoietic angiogenic cells which are not endothelial progenitors. In this review, we seek to discourage the indiscriminate use of "EPCs," and instead propose precise terminology based on defining cellular phenotype and function...
May 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28281760/modeling-closed-equilibrium-systems-of-h2o-dissolved-co2-solid-caco3
#20
Toomas Tenno, Kalev Uiga, Alexsey Mashirin, Ivar Zekker, Ergo Rikmann
In many places in the world, including North Estonia, the bedrock is limestone, which consists mainly of CaCO3. Equilibrium processes in water involving dissolved CO2 and solid CaCO3 play a vital role in many biological and technological systems. The solubility of CaCO3 in water is relatively low. Depending on the concentration of dissolved CO2, the solubility of CaCO3 changes, which determines several important ground- and wastewater parameters, for example, Ca(2+) concentration and pH. The distribution of ions and molecules in the closed system solid H2O-dissolved CO2-solid CaCO3 is described in terms of a structural scheme...
April 12, 2017: Journal of Physical Chemistry. A
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