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Meeri Visnapuu, Merilin Rosenberg, Egle Truska, Ergo Nõmmiste, Andris Šutka, Anne Kahru, Mihkel Rähn, Heiki Vija, Kaja Orupõld, Vambola Kisand, Angela Ivask
Application of efficient antimicrobial surfaces has been estimated to decrease both, the healthcare-associated infections and the spread of antibiotic-resistant bacteria. In this paper, we prepared ZnO and ZnO/Ag nanoparticle covered surfaces and evaluated their antimicrobial efficacy towards a Gram-negative bacterial model (Escherichia coli), a Gram-positive bacterial model (Staphylococcus aureus) and a fungal model (Candida albicans) in the dark and under UVA illumination. The surfaces were prepared by spin coating aliquots of ZnO and ZnO/Ag nanoparticle suspensions onto glass substrates...
May 4, 2018: Colloids and Surfaces. B, Biointerfaces
Martin A Newman, Fei Ji, Sylvia E J Fischer, Anthony Anselmo, Ruslan I Sadreyev, Gary Ruvkun
RNAi pathways detect and silence foreign nucleic acids such as viruses as well as endogenous genes in many species. The phylogenetic profile across eukaryotes of proteins that mediate key steps in RNAi is correlated with the profiles of multiple mRNA splicing proteins and with intron number, suggesting that RNAi may surveil mRNA splicing to detect the divergent or absent introns of viruses. Here we examine the role of mRNA splicing in Caenorhabditis elegans RNAi . We found that viable null mutations in U1 and U2 small nuclear ribonucleic protein (snRNP)-specific splicing factor genes cause defects in RNAi...
May 8, 2018: Genes & Development
Prashanth S Narayan, Nagappa L Teradal, Seetharamappa Jaldappagari, Ashis K Satpati
Graphene oxide (GO) was synthesized and characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA). GO was then electrochemically reduced and used for electrochemical study of mycophenolate mofetil (MMF). The electrochemically reduced graphene oxide (ERGO) film on glassy carbon electrode (GCE) showed enhanced peak current for electrooxidation of MMF...
April 2018: Journal of Pharmaceutical Analysis
F C Mota, I Cecconello, F R Takeda, F Tustumi, R A A Sallum, W M Bernardo
BACKGROUND: Esophageal carcinoma usually shows poor long-term survival rates, even when esophagectomy, the standard curative treatment is performed. As a result, there has been increasing interest in the neoadjuvant therapy, which could potentially downstage cancer, eliminate micrometastasis and ergo increase resectability and curative (R0) resection. Currently, for the earliest stage esophageal cancers, most guidelines point out to the role of endoscopic treatment, and for T1bN0 upfront surgery...
May 3, 2018: International Journal of Surgery
Jahangir Ahmad Rather, Emad A Khudaish, Palanisamy Kannan
We report the construction of a novel electrochemical femtomolar aptasensing APT-ERGO/GCE interface based on the covalent immobilization of 38-mer amine-functionalized (NH2-APT) 17β-estradiol (E2) DNA aptamers on a graphene amplifying platform. Graphene oxide (GO) was synthesized and characterized by using FTIR, UV-vis spectroscopy, XRD spectroscopy, and SEM technique. The strategy for the construction of the E2-aptasensing interface involves in a three-step modification process. (i) First, we carried out the electrochemical reduction of GO on the GCE electrode to form ERGO/GCE...
April 6, 2018: Analyst
Elizabeth Urban
In this paper the author considers Descartes' place in current thinking about the mind-body dilemma. The premise here is that in the history of ideas, the questions posed can be as significant as the answers acquired. Descartes' paramount question was 'How do we determine certainty?' and his pursuit of an answer led to cogito ergo sum. His discovery simultaneously raised the question whether mind is separate from or unified with the body. Some who currently hold that brain and subjectivity are unified contend that the philosopher 'split' mind from body and refer to 'Descartes' error'...
April 2018: Journal of Analytical Psychology
M Z H Khan, Xiaoqiang Liu, J Zhu, F Ma, W Hu, Xiuhua Liu
This work reports the development of a simple and cost-effective electrochemical sensor based on electrochemically reduced graphene oxide (ErGO) bound to the surface of indium tin oxide (ITO) electrode through 3-aminopropyltriethoxysilane (APTES) monolayer (ITO/APTES/ErGO) for selective and sensitive detection of tyramine. The features of the modified electrode were studied by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance (EIS) methods. The analytical performance of the developed sensor was evaluated with respect to linear detection range, limit of detection, stability, coexistence interference and reproducibility...
June 15, 2018: Biosensors & Bioelectronics
Daniel P Reich, Katarzyna M Tyc, Brenda L Bass
Cellular dsRNAs are edited by adenosine deaminases that act on RNA (ADARs). While editing can alter mRNA-coding potential, most editing occurs in noncoding sequences, the function of which is poorly understood. Using dsRNA immunoprecipitation (dsRIP) and RNA sequencing (RNA-seq), we identified 1523 regions of clustered A-to-I editing, termed editing-enriched regions (EERs), in four stages of Caenorhabditis elegans development, often with highest expression in embryos. Analyses of small RNA-seq data revealed 22- to 23-nucleotide (nt) siRNAs, reminiscent of viral siRNAs, that mapped to EERs and were abundant in adr-1;adr-2 mutant animals...
February 1, 2018: Genes & Development
Christian Lerch, Rukshana Shroff, Mandy Wan, Lesley Rees, Helen Aitkenhead, Ipek Kaplan Bulut, Daniela Thurn, Aysun Karabay Bayazit, Anna Niemirska, Nur Canpolat, Ali Duzova, Karolis Azukaitis, Ebru Yilmaz, Fatos Yalcinkaya, Jerome Harambat, Aysel Kiyak, Harika Alpay, Sandra Habbig, Ariane Zaloszyc, Oguz Soylemezoglu, Cengiz Candan, Alejandra Rosales, Anette Melk, Uwe Querfeld, Maren Leifheit-Nestler, Anja Sander, Franz Schaefer, Dieter Haffner
Background: We investigated the effects of nutritional vitamin D supplementation on markers of bone and mineral metabolism, i.e. serum levels of fibroblast growth factor 23 (FGF23), Klotho, bone alkaline phosphatase (BAP) and sclerostin, in two cohorts with chronic kidney disease (CKD). Methods: In all, 80 vitamin D-deficient children were selected: 40 with mild to moderate CKD from the ERGO study, a randomized trial of ergocalciferol supplementation [estimated glomerular filtration rate (eGFR) 55 mL/min/1...
February 21, 2018: Nephrology, Dialysis, Transplantation
Isabelle Vivodtzev, Renaud Tamisier, Marilie Croteau, Jean-Christian Borel, Angélique Grangier, Bernard Wuyam, Patrick Lévy, Caroline Minville, Frédéric Sériès, François Maltais, Jean-Louis Pépin
BACKGROUND: Obstructive sleep apnoea (OSA) and obesity are interdependent chronic diseases sharing reduced exercise tolerance and high cardiovascular risk. INTERVENTION: A 3-month intervention with innovative training modalities would further improve functional capacity and cardiovascular health than usual cycle exercise training in already continuous positive airway pressure (CPAP)-treated obese patients with OSA. METHODS: Fifty three patients (35<body mass index (BMI) <45 kg/m2 ) were randomly allocated to exercise training on a cycle ergometer, either alone (ERGO) or with respiratory muscle training (ERGO+RMT) or non-invasive ventilation (ERGO+NIV)...
February 20, 2018: Thorax
Pramod Shinde, Camellia Sarkar, Sarika Jalan
The nucleotide polymorphism in the human mitochondrial genome (mtDNA) tolled by codon position bias plays an indispensable role in human population dispersion and expansion. Herein, genome-wide nucleotide co-occurrence networks were constructed using data comprised of five different geographical regions and around 3000 samples for each region. We developed a powerful network model to describe complex mitochondrial evolutionary patterns among codon and non-codon positions. We found evidence that the evolution of human mitochondria DNA is dominated by adaptive forces, particularly mutation and selection, which was supported by many previous studies...
February 15, 2018: Scientific Reports
Srinivasan Kesavan, Deivasigamani Ranjith Kumar, Marjorie Lara Baynosa, Jae-Jin Shim
An electrode comprised of a polydiaminobenzene (p-DAB) film formed on electrochemically reduced graphene oxide (ERGO) on a glassy carbon (GC) (p-DAB@ERGO/GC) was fabricated using a potentiodynamic method for the sensitive and selective determination of nitrite in the presence of a common interference. The p-DAB@ERGO/GC film-modified electrode surfaces were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry...
April 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
G Ibáñez-Redín, D Wilson, D Gonçalves, O N Oliveira
A green approach for the preparation of carbon black (CB) and electrochemically reduced graphene oxide composite (ERGO) is described based on screen printed carbon electrodes (SPCEs) fabricated on poly(ethylene terephthalate) (PET) as electrochemical sensors. This approach leads to a heterogeneous hydrophilic surface with high concentration of defect sites according to scanning electron microscopy, contact angle and Raman spectroscopy measurements. The SPCE/CB-ERGO sensor was tested with dopamine (DA), epinephrine (EP) and paracetamol (PCM), exhibiting an enhanced electrocatalytic performance compared to the bare SPCE...
April 1, 2018: Journal of Colloid and Interface Science
Devaraj Manoj, K Theyagarajan, Duraisamy Saravanakumar, Sellappan Senthilkumar, Kathavarayan Thenmozhi
An aldehyde functionalized ionic liquid, (3-(3-formyl-4-hydroxybenzyl)-3-methylimidazolium hexafluorophosphate) (CHO-IL) has been employed herein as a multiple host platform for the covalent immobilization of mediator as well as enzyme. The CHO-IL was immobilized on electrochemically reduced graphene oxide (ERGO) through the π-π stacking of hydroxybenzyl and imidazolium groups with ERGO and subjected to further covalent attachment of Azure A (Azu-A) mediator or glucose oxidase (GOx) enzyme. Electroactive, water soluble organic dye Azu-A was effectively immobilized to the host IL through simple Schiff base reaction...
April 30, 2018: Biosensors & Bioelectronics
Marta Berholts, Hanna Myllynen, Kuno Kooser, Eero Itälä, Sari Granroth, Helena Levola, Joakim Laksman, Shabnam Oghbaiee, Bart Oostenrijk, Ergo Nõmmiste, Edwin Kukk
Photofragmentation of gas-phase acetamide and acetic acid clusters produced by a supersonic expansion source has been studied using time-of-flight mass spectrometry and the partial ion yield (PIY) technique combined with tunable vacuum-ultraviolet synchrotron radiation. Appearance energies of the clusters and their fragments were experimentally determined from the PIY measurements. The effect of clusterization conditions on the formation and fragmentation of acetic acid clusters was investigated. Ab initio quantum mechanical calculations were performed on both samples' dimers to find their neutral and ionized geometries as well as proton transfer energy barriers leading to the optimal geometries...
November 21, 2017: Journal of Chemical Physics
Eun Sung Jung, Hye Min Park, Seung Min Hyun, Jong Cheol Shon, Digar Singh, Kwang-Hyeon Liu, Tae Woong Whon, Jin-Woo Bae, Jae Sung Hwang, Choong Hwan Lee
The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-exposed mice groups (UVB), class Bacilli and order Bifidobacteriales were observed as discriminant taxa with decreased lysophospholipid levels compared to the unexposed mice groups subjected to normal diet (NOR)...
2017: PloS One
Dinakaran Thirumalai, Vijayaraj Kathiresan, Jaewon Lee, Sung-Ho Jin, Seung-Cheol Chang
A new amperometric biosensor for hydrogen peroxide (H2O2) and superoxide anion (SO) has been developed. The biosensor developed uses cytochrome c (Cyt c) modified glassy carbon electrodes coupled with electrochemically reduced graphene oxide (ErGO). To immobilize Cyt c, the "one step" electrochemical deposition of vertically aligned and ErGO (VAErGO) has been performed by using a pulse reverse technique, thus resulting in a very simple and efficient system. The well-established vertical alignment of ErGO was confirmed by atomic force microscopy and the electrochemical characteristics of the biosensor were investigated by cyclic voltammetric, electrochemical impedance spectroscopy and amperometric techniques...
November 8, 2017: Analyst
Sebastião Barbosa-Netto, Obanshe S dʼAcelino-E-Porto, Marcos B Almeida
Barbosa-Netto, S, d'Acelino-e-Porto, OS, and Almeida, MB. Self-selected resistance exercise load: implications for research and prescription. J Strength Cond Res XX(X): 000-000, 2017-Resistance training has been widely used for maintaining or improving the quality of life and sports performance. It is not clear, however, whether the load commonly used in these exercises is equivalent to the number of repetitions determined for execution, that is, the level of effort applied to the self-selected load. Ergo, the aim of this study was to identify the number of maximum repetitions that strength training practitioners can perform with the load commonly used (self-selected) to perform 10 repetitions in their training routines...
November 1, 2017: Journal of Strength and Conditioning Research
Guangming Nie, Yang Wang, Yun Tang, Dan Zhao, Qingfu Guo
A novel electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of carcinoembryonic antigen (CEA) was developed using signal amplification strategy based on poly(5-formylindole)/reduced graphene oxide nanocomposite (P5FIn/erGO) and Au nanoparticle (AuNP) decorated graphene quantum dots (GQDs) (GQDs@AuNP). As an effective matrix for immobilization of primary antibody (Ab1), P5FIn/erGO nanocomposite facilitated the ion transport during the redox reactions and provided larger surface areas for the immobilization of Ab1...
October 12, 2017: Biosensors & Bioelectronics
Stijn De Baets, Patrick Calders, Noortje Schalley, Katrien Vermeulen, Sofie Vertriest, Lien Van Peteghem, Marieke Coussens, Fransiska Malfait, Guy Vanderstraeten, Geert Van Hove, Dominique Van de Velde
Objectives To synthesize the evidence on the psychometrics functional capacity evaluation (FCE) methods. Methods A systematic literature search in nine databases. The resulting articles were screened based on predefined in- and exclusion criteria. Two reviewers independently performed this screening. Included studies were appraised based on their methodological quality. Results The search resulted in 20 eligible studies about nine different FCE methods. The Baltimore Therapeutic Equipment work simulator showed a moderate predictive validity...
October 7, 2017: Journal of Occupational Rehabilitation
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