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https://www.readbyqxmd.com/read/28820312/architecture-of-the-atg2b-wdr45-complex-and-an-aromatic-y-hf-motif-crucial-for-complex-formation
#1
Jing-Xiang Zheng, Yan Li, Yue-He Ding, Jun-Jie Liu, Mei-Jun Zhang, Meng-Qiu Dong, Hong-Wei Wang, Li Yu
PtdIns3P signaling is critical for dynamic membrane remodeling during autophagosome formation. Proteins in the Atg18/WIPI family are PtdIns3P-binding effectors which can form complexes with proteins in the Atg2 family, and both families are essential for macroautophagy/autophagy. However, little is known about the biophysical properties and biological functions of the Atg2-Atg18/WIPI complex as a whole. Here, we demonstrate that an ortholog of yeast Atg18, mammalian WDR45/WIPI4 has a stronger binding capacity for mammalian ATG2A or ATG2B than the other 3 WIPIs...
August 18, 2017: Autophagy
https://www.readbyqxmd.com/read/28819645/3d-imaging-of-optically-cleared-tissue-using-a-simplified-clarity-method-and-on-chip-microscopy
#2
Yibo Zhang, Yoonjung Shin, Kevin Sung, Sam Yang, Harrison Chen, Hongda Wang, Da Teng, Yair Rivenson, Rajan P Kulkarni, Aydogan Ozcan
High-throughput sectioning and optical imaging of tissue samples using traditional immunohistochemical techniques can be costly and inaccessible in resource-limited areas. We demonstrate three-dimensional (3D) imaging and phenotyping in optically transparent tissue using lens-free holographic on-chip microscopy as a low-cost, simple, and high-throughput alternative to conventional approaches. The tissue sample is passively cleared using a simplified CLARITY method and stained using 3,3'-diaminobenzidine to target cells of interest, enabling bright-field optical imaging and 3D sectioning of thick samples...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28810611/three-dimensional-spiral-ct-measurement-of-atlantal-pedicle-and-its-clinical-application
#3
Wengui Xie, Pengfei Gao, Lixin Ji
The study aimed to establish the safe placement area and corresponding entry angle of atlantal pedicle screw using axial computed tomography (CT) measurement of atlas, in order to guide the clinical operation. Spiral thin-slice CT scan of atlas and three-dimensional reconstruction of 38 patients were randomly selected. Screw placement space was defined as the distance between the tangent lines of entry channel on the atlantal cross section and inner edge of transverse foramen and outer edge of spinal canal...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28809707/a-streaming-pca-vlsi-chip-for-neural-data-compression
#4
Tong Wu, Wenfeng Zhao, Hongsun Guo, Hubert H Lim, Zhi Yang
Neural recording system miniaturization and integration with low-power wireless technologies require compressing neural data before transmission. Feature extraction is a procedure to represent data in a low-dimensional space; its integration into a recording chip can be an efficient approach to compress neural data. In this paper, we propose a streaming principal component analysis algorithm and its microchip implementation to compress multichannel local field potential (LFP) and spike data. The circuits have been designed in a 65-nm CMOS technology and occupy a silicon area of 0...
August 14, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28807205/facilitating-surgeon-understanding-of-complex-anatomy-using-a-three-dimensional-printed-model
#5
Barrett P Cromeens, William C Ray, Brad Hoehne, Fikir Abayneh, Brent Adler, Gail E Besner
BACKGROUND: 3-dimensional prints (3DP) anecdotally facilitate surgeon understanding of anatomy and decision-making. However, the actual benefit to surgeons or patients has not been quantified. This study investigates how surgeon understanding of complex anatomy is altered by a 3DP compared to computed tomography (CT) scan or CT + digital reconstruction (CT + DR). MATERIALS AND METHODS: Key anatomic features were segmented from a CT-abdomen/pelvis of pygopagus twins to build a DR and printed in color on a 3D printer...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28800252/progressive-changes-in-walking-kinematics-and-kinetics-after-anterior-cruciate-ligament-injury-and-reconstruction-a-review-and-meta-analysis
#6
Lindsay V Slater, Joseph M Hart, Adam R Kelly, Christopher M Kuenze
CONTEXT:   Anterior cruciate ligament (ACL) injury and ACL reconstruction (ACLR) result in persistent alterations in lower extremity movement patterns. The progression of lower extremity biomechanics from the time of injury has not been described. OBJECTIVE:   To compare the 3-dimensional (3D) lower extremity kinematics and kinetics of walking among individuals with ACL deficiency (ACLD), individuals with ACLR, and healthy control participants from 3 to 64 months after ACLR...
August 11, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28796072/one-visualization-simulation-operation-system-for-distal-femoral-fracture
#7
Dan Wang, Ning Li, Ming Luo, Yao-Kun Chen
A computer-aided 3-dimensional (3D) visualization operation simulation system based on computer-aided design (CAD) Unigraphics NX and Mimics software was established to provide orthopedic surgeons with an actual and reliable system in treating of distal femoral fracture.According to the preoperative CT data, 3D reconstruction of the distal femoral fracture could be achieved by the Mimics software. Then, the CAD Unigraphics NX software was used to measure the model function of all the related surgical instruments, including less invasive stabilization system (LISS) and retrograde intramedullary nail fixation...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28795555/chemical-imaging-of-self-assembled-monolayers-on-copper-using-compressive-hyperspectral-sum-frequency-generation-microscopy
#8
Desheng Zheng, Liyang Lu, Kevin F Kelly, Steven Baldelli
Sum frequency generation microscopy is a label-free optical imaging technique with intrinsic molecular vibrational contrast for surface studies. Recent developments of compressive sensing broad-band hyperspectral SFG microscopy have demonstrated the potential application for imaging monolayer at metal surfaces with micrometer spatial resolution. Here is presented the capability of chemical imaging of spatially patterned monolayers of 1-octadecanethiol (ODT) and 16-methoxy-1-hexadecanethiol (MeOHT) molecules assembled on a copper surface...
August 10, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28795038/indentation-in-the-right-ventricle-by-an-incomplete-pericardium-on-3-dimensional-reconstructed-computed-tomography
#9
Hak Ju Kim, Jae Gun Kwak
We report the case of a 17-year-old girl who presented with an indentation in the right ventricle caused by an incomplete pericardium on preoperative 3-dimensional reconstructed computed tomography. She was to undergo surgery for a partial atrioventricular septal defect and secundum atrial septal defect. Preoperative electrocardiography revealed occasional premature ventricular beats. We found the absence of the left side of the pericardium intraoperatively, and this absence caused strangulation of the diaphragmatic surface of the right ventricle...
August 2017: Korean Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28792664/collagen-coated-nano-electrospun-pcl-seeded-with-human-endometrial-stem-cells-for-skin-tissue-engineering-applications
#10
Shiva Sharif, Jafar Ai, Mahmoud Azami, Javad Verdi, Mohammad Ali Atlasi, Sadegh Shirian, Ali Samadikuchaksaraei
Human endometrial stem cells (hEnSCs) are known as an attractive source of stem cells for regenerative medicine. hEnSCs are easily isolated and are capable of repairing uterine through their strong ability of creating new capillaries. In this study, a three-dimensional (3D) nanofibrous polycaprolactone (PCL)/collagen scaffold was fabricated and characterized in order to be applied as a new approach for skin reconstruction. Furthermore, the behavior of hEnSCs on this scaffold was investigated. First, a PCL 3D scaffold was constructed using electrospinning technique...
August 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28790477/pediatric-lumbar-pedicle-screw-placement-using-navigation-templates-a-cadaveric-study
#11
Xing Wang, Jun Shi, Shojie Zhang, Zhifeng Zhang, Xiaohe Li, Zhijun Li
BACKGROUND: Pedicle screw technique is commonly used treatment of adult spinal trauma, tumor, degeneration. The application of pedicle screws is very challenging in children because children have a fast growing spine and spinal pedicle morphology of children and adult has large difference. 3 D reconstruction individual navigation templates improve the success rate of pediatric pedicle screw system. This study is aimed to provide a precise method for lumbar spine pedicle screw placement in children using computer-aided design and rapid prototyping technology...
July 2017: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/28782129/volumetric-mri-thermometry-using-a-three-dimensional-stack-of-stars-echo-planar-imaging-pulse-sequence
#12
Sumeeth V Jonathan, William A Grissom
PURPOSE: To measure temperature over a large brain volume with fine spatiotemporal resolution. METHODS: A three-dimensional stack-of-stars echo-planar imaging sequence combining echo-planar imaging and radial sampling with golden angle spacing was implemented at 3T for proton resonance frequency-shift temperature imaging. The sequence acquires a 188x188x43 image matrix with 1.5x1.5x2.75 mm(3) spatial resolution. Temperature maps were reconstructed using sensitivity encoding (SENSE) image reconstruction followed by the image domain hybrid method, and using the k-space hybrid method...
August 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28780569/three-dimensional-analysis-of-seminiferous-tubules-and-spermatogenic-waves-in-mice
#13
Hiroki Nakata, Takahiro Sonomura, Shoichi Iseki
The aim of the present study was to reconstruct seminiferous tubules and analyze spermatogenic waves in seminiferous epithelia in developing and adult mice using serial paraffin sections and high-performance three-dimensional (3D) reconstruction software. By labeling the basement membrane of seminiferous tubules with fluorescent immunohistochemistry or periodic acid-Schiff-hematoxylin staining, all seminiferous tubules were reconstructed in 9 testes from 9 different mice, 3 each at 0, 21, and 90 days (adult) postpartum...
August 5, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28780107/validation-of-2-noninvasive-markerless-reconstruction-techniques-in-biplane-high-speed-fluoroscopy-for-3-dimensional-research-of-bovine-distal-limb-kinematics
#14
M Weiss, E Reich, S Grund, C K W Mülling, S M Geiger
Lameness severely impairs cattle's locomotion, and it is among the most important threats to animal welfare, performance, and productivity in the modern dairy industry. However, insight into the pathological alterations of claw biomechanics leading to lameness and an understanding of the biomechanics behind development of claw lesions causing lameness are limited. Biplane high-speed fluoroscopic kinematography is a new approach for the analysis of skeletal motion. Biplane high-speed videos in combination with bone scans can be used for 3-dimensional (3D) animations of bones moving in 3D space...
August 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28777285/3d-modeling-of-lower-extremities-with-biplanar-radiographs-reliability-of-measures-on-subsequent-examinations
#15
David E Westberry, Ashley M Carpenter
BACKGROUND: Biplanar radiography with 3-dimensional (3D) modeling (EOS) provides a comprehensive assessment of lower limb alignment in an upright weight-bearing position with less radiation than conventional radiography. A study was performed to assess the consistency and reliability of 2 lower extremity 3D biplanar radiograph models created at least 1 year apart in a pediatric population. METHODS: All patients who had 2 lower extremity radiographic evaluations with EOS performed at visits a minimum of 1 year apart were reviewed...
August 2, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28769763/development-of-serotonergic-fibers-in-the-post-natal-mouse-brain
#16
Giacomo Maddaloni, Alice Bertero, Marta Pratelli, Noemi Barsotti, Annemarie Boonstra, Andrea Giorgi, Sara Migliarini, Massimo Pasqualetti
Serotonin (5-HT)-synthetizing neurons, which are confined in the raphe nuclei of the rhombencephalon, provide a pervasive innervation of the central nervous system (CNS) and are involved in the modulation of a plethora of functions in both developing and adult brain. Classical studies have described the post-natal development of serotonergic axons as a linear process of terminal field innervation. However, technical limitations have hampered a fine morphological characterization. With the advent of genetic mouse models, the possibility to label specific neuronal populations allowed the rigorous measurement of their axonal morphological features as well as their developmental dynamics...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28767166/radiological-anatomy-of-the-perforators-of-the-gluteal-region-the-radiosome-based-anatomy
#17
Enrico Vigato, Eleonora De Antoni, Cesare Tiengo, Andrea Porzionato, Cinzia Tortorella, Maurizio Governa, Veronica Macchi, Raffaele De Caro
BACKGROUND: The superior (SGA) and the inferior gluteal artery (IGA) perforator flaps are widely used in pressure-sore repair and in breast reconstruction. The aim was to exhaustively depict the topographical anatomy of the whole system of perforators in the buttock. METHODS: Eighty lower-extremity computed tomographic angiography (CTA) of patients (20 males/20 females, mean age 61-years old, range 38-81) were considered. The source artery, location, type, and caliber of gluteal perforators were analyzed...
August 2, 2017: Microsurgery
https://www.readbyqxmd.com/read/28766912/measurement-of-the-zygomatic-region-for-the-optimal-placement-of-quad-zygomatic-implants
#18
Kuo-Feng Hung, Qi-Yong Ai, Sheng-Chi Fan, Feng Wang, Wei Huang, Yi-Qun Wu
BACKGROUND: The zygomatic implant is suggested to be placed in zygomatic areas with the largest thickness, but regions to obtain the largest bone-to-implant contact (BIC) were unknown. PURPOSE: To identify the zygomatic regions for placing quad zygomatic implants that obtain the largest BIC. MATERIALS AND METHODS: The zygomatic thicknesses of 300 zygomata were measured at total 12 points on the superior, middle, and inferior areas on the 3-dimensional reconstruction images...
August 1, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28766344/cell-shaping-micropatterns-for-quantitative-super-resolution-microscopy-imaging-of-membrane-mechanosensing-proteins
#19
Anthony Fernandez, Markville Bautista, Ramunas Stanciauskas, Taerin Chung, Fabien Pinaud
Patterning cells on microcontact-printed substrates is a powerful approach to control cell morphology and introduce specific mechanical cues on a cell's molecular organization. Although global changes in cellular architectures caused by micropatterns can easily be probed with diffraction-limited optical microscopy, studying molecular reorganizations at the nanoscale demands micropatterned substrates that accommodate the optical requirements of single molecule microscopy techniques. Here, we developed a simple micropatterning strategy that provides control of cellular architectures and is optimized for nanometer accuracy single molecule tracking and three-dimensional super-resolution imaging of plasma and nuclear membrane proteins in cells...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28765804/integrated-fixation-cage-loosening-under-fatigue-loading
#20
Srinidhi Nagaraja, Vivek Palepu
BACKGROUND: Screw loosening is a well-known adverse event in traditional spinal fusion instrumentation. This phenomenon may hinder segmental stability of the spine leading to bony non-union. In recent years numerous lumbar integrated fixation cages (IFC) have been introduced that offer a low profile alternative to a standard cage with an anterior plate (AP+C). The fixation approach for IFCs is different than a traditional anterior approach; therefore, it is unclear whether IFCs may loosen from the surrounding bone over time...
2017: International Journal of Spine Surgery
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