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https://www.readbyqxmd.com/read/29241222/flavopiridol-enhances-abt-199-sensitivity-in-unfavourable-risk-multiple-myeloma-cells-in-vitro-and-in-vivo
#1
Liang Zhou, Yu Zhang, Deepak Sampath, Joel Leverson, Yun Dai, Maciej Kmieciak, Matthew Nguyen, Robert Z Orlowski, Steven Grant
BACKGROUND: The BCL-2-specific BH3-mimetic ABT-199 (venetoclax) has been reported to be principally active against favourable-risk multiple myeloma (MM) cells, prompting efforts to extend its activity to include more resistant, higher-risk MM subsets. METHODS: Effects of the CDK9 inhibitor flavopiridol (FP; alvocidib) on responses to ABT-199 were examined in MM cells. Cell death and protein expression were evaluated by western blot and immunofluorescence. Xenograft models were used to study combination effects in vivo...
December 14, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29241186/strengthening-gastric-cancer-therapy-by-trastuzumab-conjugated-nanoparticles-with-simultaneous-encapsulation-of-anti-mir-21-and-5-fluorouridine
#2
Nan Hu, Jun Feng Yin, Ze Ji, Yidong Hong, Puyuan Wu, Baoxiang Bian, Ziyan Song, Rutian Li, Qin Liu, Fenglei Wu
BACKGROUND/AIMS: MicroRNA-21 is an oncogenic miR (oncomiR) frequently elevated in gastric cancer (GC). Overexpression of miR-21 decreases the sensitivity of GC cells to 5-fluorouridine (5-Fu) and trastuzumab, a humanized monoclonal antibody targeting human epidermal growth factor receptor 2 (HER2). Receptor-mediated endocytosis plays a crucial role in the delivery of biotherapeutics including anti-miRNA oligonucleotides (AMOs). This study is a continuation of earlier findings involving poly(ε-caprolactone) (PCL)-poly (ethylene glycol) (PEG) nanoparticles (PEG-PCL NPs), which were coated with trastuzumab to target GC with HER2 receptor over-expression using anti-miRNA-21 (AMO-21) and 5-Fu...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29240919/genome-wide-dose-dependent-inhibition-of-histone-deacetylases-studies-reveal-their-roles-in-enhancer-remodeling-and-suppression-of-oncogenic-super-enhancers
#3
Gilson J Sanchez, Phillip A Richmond, Eric N Bunker, Samuel S Karman, Joseph Azofeifa, Aaron T Garnett, Quanbin Xu, Graycen E Wheeler, Cathryn M Toomey, Qinghong Zhang, Robin D Dowell, Xuedong Liu
Histone deacetylase inhibitors (HDACIs) are known to alter gene expression by both up- and down-regulation of protein-coding genes in normal and cancer cells. However, the exact regulatory mechanisms of action remain uncharacterized. Here we investigated genome wide dose-dependent epigenetic and transcriptome changes in response to HDACI largazole in a transformed and a non-transformed cell line. Exposure to low nanomolar largazole concentrations (<GI50) predominantly resulted in upregulation of gene transcripts whereas higher largazole doses (≥GI50) triggered a general decrease in mRNA accumulation...
December 12, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29240897/factors-influencing-women-in-physical-activity-programs-in-malaysia
#4
C Hanlon, S Khoo, T Morris, R Eime
The majority of research on factors associated with women participation in physical activity (PA) has been in developed countries with limited research in developing countries. Few women in Malaysia are active at the recommended levels for health, and activity rates are less than developed countries. Little research has focused specially on physically active Malaysian women and the factors that contribute to them becoming and staying active in PA programs. This lack of knowledge hinders the tailored development and implementation of PA programs to meet their needs...
December 11, 2017: Health Promotion International
https://www.readbyqxmd.com/read/29240830/a-low-fat-diet-ameliorates-pathology-but-retains-beneficial-effects-associated-with-cpt1b-knockout-in-skeletal-muscle
#5
Jaycob D Warfel, Bolormaa Vandanmagsar, Shawna E Wicks, Jingying Zhang, Robert C Noland, Randall L Mynatt
Inhibiting fatty acid oxidation is one approach to lowering glucose levels in diabetes. Skeletal muscle specific Carnitine Palmitoyltransferase 1b knockout mice (Cpt1bm-/-) comprise a model of impaired fat oxidation; and have decreased fat mass and enhanced glucose disposal and muscle oxidative capacity compared to controls. However, unfavorable effects occur relative to controls when Cpt1bm-/- mice are fed a 25% fat diet, including decreased activity and fat free mass and increased intramuscular lipid and serum myoglobin...
2017: PloS One
https://www.readbyqxmd.com/read/29240807/diversification-of-the-insulin-like-growth-factor-1-gene-in-mammals
#6
Peter Rotwein
Insulin-like growth factor 1 (IGF1), a small, secreted peptide growth factor, is involved in a variety of physiological and patho-physiological processes, including somatic growth, tissue repair, and metabolism of carbohydrates, proteins, and lipids. IGF1 gene expression appears to be controlled by several different signaling cascades in the few species in which it has been evaluated, with growth hormone playing a major role by activating a pathway involving the Stat5b transcription factor. Here, genes encoding IGF1 have been evaluated in 25 different mammalian species representing 15 different orders and ranging over ~180 million years of evolutionary diversification...
2017: PloS One
https://www.readbyqxmd.com/read/29240260/sohlh1-suppresses-glioblastoma-cell-proliferation-migration-and-invasion-by-inhibition-of-wnt-%C3%AE-catenin-signaling
#7
Xinyu Liu, Qing Gao, Na Zhao, Xiaoli Zhang, Weiwei Cui, Jinhao Sun, Jiang Fu, Jing Hao
Glioblastoma (GBM) is the most aggressive and highly invasive type of astrocytic tumors. Despite advances in diagnosis and therapy, the prognosis and survival time remain poor. Identifying key mediators of tumor cell proliferation, migration and invasion is crucial to the development of new and more effective therapies. In this paper, we report the novel role of Spermatogenesis- and oogenesis- specific basic helix-loop-helix transcription factor1 (Sohlh1) in the inhibition of Wnt/β-catenin signaling and aggressive behaviors in GBM cells...
December 14, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29240236/evaluation-of-osteoblast-differentiation-and-function-when-cultured-on-mesoporous-bioactive-glass-adsorbed-with-testosterone
#8
Kai Gao, Xiaoyan Wang, Qianqian Liu, Wei Chen, Gan Wang, Dongyi Zhang, Long Liu
Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure and large pore volume. Here we reported that the proliferation, differentiation and mineralization were promoted in MC3T3-E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3-E1 cells cultured on MBG/T...
December 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29239726/camp-signaling-regulates-dna-hydroxymethylation-by-augmenting-the-intracellular-labile-ferrous-iron-pool
#9
Vladimir Camarena, David W Sant, Tyler C Huff, Sushimita Mustafi, Ryan K Muir, Allegra T Aron, Christopher J Chang, Adam R Renslo, Paula Monje, Gaofeng Wang
It is widely accepted that cAMP regulates gene transcription principally by activating the protein kinase A (PKA)-targeted transcription factors. Here, we show that cAMP enhances the generation of 5-hydroxymethylcytosine (5hmC) in multiple cell types. 5hmC is converted from 5-methylcytosine (5mC) by Tet methylcytosine dioxygenases, for which Fe(II) is an essential cofactor. The promotion of 5hmC was mediated by a prompt increase of the intracellular labile Fe(II) pool (LIP). cAMP enhanced the acidification of endosomes for Fe(II) release to the LIP likely through RapGEF2...
December 14, 2017: ELife
https://www.readbyqxmd.com/read/29239473/polyamines-regulating-phytic-acid-degradation-in-mung-bean-sprouts
#10
Ting Zhou, Pei Wang, Runqiang Yang, Zhenxin Gu
BACKGROUND: Polyamines are essentially involved in cell division and differentiation. Transport of polyamine is ATP dependent, while phytic acid is the major reserve of phosphate essential to the energy-producing machinery of cells. Thus, polyamines might enhance phytic acid degradation during mung bean germination. In this study, different polyamines [putrescine (Put), spermidine (Spd) and spermine (Spm)] and dicyclohexylamine (DCHA, a Spd synthesis inhibitor) were applied to investigate the function of polyamines on phytic acid degradation...
December 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29239279/radiosensitization-by-cpg-odn7909-in-an-epidermoid-laryngeal-carcinoma-hep-2-cell-line
#11
Shu Wang, Xiaoqun Liu, Tiankui Qiao, Qi Zhang
Objective To evaluate the radiosensitivity effect of CpG oligodeoxyribonucleotide (ODN) 7909 on human epidermoid cancer strain-2 (Hep-2) cells in vitro and discuss the potential for improved radiotherapy treatment in patients with laryngeal squamous cell carcinoma. Methods Toll-like receptor ( TLR) 9 expression was assessed in Hep-2 cells using Western blots and reverse transcription polymerase chain reaction. Cell Counting Kit-8 was used to detect Hep-2 cell viability at 24 and 48 h following treatment with different CpG ODN7909 concentrations...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/29238954/il-25-is-involved-in-ctcl-progression-by-establishing-th2-dominant-microenvironment
#12
R Nakajima, T Miyagaki, T Oka, N Takahashi, M Hirakawa, H Suga, A Yoshizaki, H Fujita, Y Asano, M Sugaya, S Sato
BACKGROUND: Interleukin (IL)-25 is a member of the IL-17 family which can promote and augment T-helper type (Th) 2 responses. The expression of IL-25 and its cognate receptor, IL-25 receptor (IL-25R), is upregulated and correlated with disease activity in Th2-associated diseases. OBJECTIVE: To examine the expression and function of IL-25 in cutaneous T-cell lymphoma (CTCL). METHODS: Expression and localization of IL-25 in lesional skin was investigated using immunohistochemistry...
December 13, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/29238701/a-novel-heat-shock-element-hse-in-entamoeba-histolytica-that-regulates-the-transcriptional-activation-of-the-ehpgp5-gene-in-the-presence-of-emetine-drug
#13
Alma Nieto, David G Pérez Ishiwara, Esther Orozco, Virginia Sánchez Monroy, Consuelo Gómez García
Transcriptional regulation of the multidrug resistance EhPgp5 gene in Entamoeba histolytica is induced by emetine stress. EhPgp5 overexpression alters the chloride-dependent currents that cause trophozoite swelling, diminishing induced programmed cell death (PCD) susceptibility. In contrast, antisense inhibition of P-glycoprotein (PGP) expression produces synchronous death of trophozoites and the enhancement of the biochemical and morphological characteristics of PCD induced by G418. Transcriptional gene regulation analysis identified a 59 bp region at position -170 to -111 bp promoter as putative emetine response elements (EREs)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29238464/curcumin-enhances-liver-sirt3-expression-in-the-rat-model-of-cirrhosis
#14
Sara Chenari, Fatemeh Safari, Ali Moradi
Objectives: Bill duct ligation (BDL) is a representative model of biliary cholestasis in animals. Curcumin has a protective effect on the liver; however, its underlying mechanisms are not completely known. This study explored the hepatoprotective activity of curcumin on hepatic damage via measuring the expression of sirtuin3 (SIRT3), AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase 1A (CPT-1A), isocitrate dehydrogenase2 (IDH2) and manganese superoxide dismutase (MnSOD) as well as the level of serum lipid profile in the BDL fibrotic rat model...
December 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29238352/overexpression-of-erf1-v-from-haynaldia-villosa-can-enhance-the-resistance-of-wheat-to-powdery-mildew-and-increase-the-tolerance-to-salt-and-drought-stresses
#15
Liping Xing, Zhaocan Di, Wenwu Yang, Jiaqian Liu, Meina Li, Xiaojuan Wang, Chaofan Cui, Xiaoyun Wang, Xiue Wang, Ruiqi Zhang, Jin Xiao, Aizhong Cao
The APETALA 2/Ethylene-responsive element binding factor (AP2/ERF) transcription factor gene family is widely involved in the biotic and abiotic stress regulation. Haynaldia villosa (VV, 2n = 14), a wild species of wheat, is a potential gene pool for wheat improvement. H. villosa confers high resistance to several wheat diseases and high tolerance to some abiotic stress. In this study, ERF1-V, an ethylene-responsive element-binding factor gene of the AP2/ERF transcription factor gene family from wild H. villosa, was cloned and characterized...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29238338/a-novel-tetr-family-transcriptional-regulator-calr3-negatively-controls-calcimycin-biosynthesis-in-streptomyces-chartreusis-nrrl-3882
#16
Lixia Gou, Tiesheng Han, Xiaoxia Wang, Jingxuan Ge, Wenxiu Liu, Fen Hu, Zhijun Wang
Calcimycin is a unique ionophoric antibiotic that is widely used in biochemical and pharmaceutical applications, but the genetic basis underlying the regulatory mechanisms of calcimycin biosynthesis are unclear. Here, we identified the calR3 gene, which encodes a novel TetR family transcriptional regulator and exerts a negative effect on calcimycin biosynthesis. Disruption of calR3 in Streptomyces chartreusis NRRL 3882 led to significantly increased calcimycin and its intermediate cezomycin. Gene expression analysis showed that the transcription of calR3 and its adjacent calT gene were dramatically enhanced (30- and 171-fold, respectively) in GLX26 (ΔcalR3) mutants compared with the wild-type strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29238192/transcriptome-wide-elucidation-of-liposomal-formulations-for-anticancer-drug-delivery
#17
Ying Li, Meng Wang, Bu-Wei Huang, Yuan Ping, Jian You, Jian-Qing Gao
Although widely used in chemotherapy, free doxorubicin (Dox) might enhance cell malignancy undesirably. Liposomal Dox (Doxlipo) has been clinically approved for the treatment of breast cancer due to reduced systematical toxicity and increased tumor targeting, yet the transcriptome-wide elucidation of the Doxlipo formulations remains elusive. To this end, we explored the impact of two Dox liposomal formulations, Doxlipo mainly containing hydrogenated soy phosphatidylcholine or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, on the transcriptional pattern of MCF-7 cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29238079/dorsal-root-ganglion-neurons-regulate-the-transcriptional-and-translational-programs-of-osteoblast-differentiation-in-a-microfluidic-platform
#18
Diana Isabel Silva, Bruno Paiva Dos Santos, Jacques Leng, Hugo Oliveira, Joëlle Amédée
Innervation by the sensory nervous system plays a key role in skeletal development and in orchestration of bone remodeling and regeneration. However, it is unclear how and in which bone cells can sensory nerves act to control these processes. Here, we show a microfluidic coculture system comprising dorsal root ganglion (DRG) neurons and mesenchymal stem cells (MSCs) that more faithfully represents the in vivo scenario of bone sensory innervation. We report that DRG neurons promote the osteogenic differentiation capacity of MSCs, by mediating the increase of alkaline phosphatase activity and the upregulation of osteoblast-specific genes...
December 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29237721/rev-erba-regulates-cyp7a1-through-repression-of-liver-receptor-homolog-1
#19
Tianpeng Zhang, Mengjing Zhao, Danyi Lu, Shuai Wang, Fangjun Yu, Lianxia Guo, Shijun Wen, Baojian Wu
REV-ERBα is a nuclear heme receptor (as known as a transcriptional repressor) that orchestrates circadian rhythms and cell metabolism. Here we investigate the role of LRH-1 in REV-ERBα regulation of CYP7A1 and cholesterol metabolism. We first characterized the tertiary amine GSK2945 as a highly specific Rev-erbα/REV-ERBα antagonist using cell-based assays and confirmed expression of Rev-erbα in mouse liver. GSK2945 treatment increased hepatic Cyp7a1 level and lowered plasma cholesterol in wild-type mice...
December 13, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29237164/mir-124-inhibits-growth-and-enhances-radiation-induced-apoptosis-in-non-small-cell-lung-cancer-by-inhibiting-stat3
#20
Mengjie Wang, Bi Meng, Yangchen Liu, Jingwen Yu, Qiaoyun Chen, Yangchen Liu
BACKGROUND/AIMS: A growing body of evidence indicates that the abnormal expression of microRNAs (miRNAs) play an important role in sensitizing the cellular response to ionizing radiation (IR). The aim of this study was to investigate whether the expression of miR-124 correlated with radiosensitivity in the context of non-small-cell lung carcinoma (NSCLC). METHODS: Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to quantify miR-124 expression in NSCLC tissues and cell lines...
December 11, 2017: Cellular Physiology and Biochemistry
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