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https://www.readbyqxmd.com/read/29779183/molecular-characterization-and-potential-synthetic-applications-of-gh1-%C3%AE-glucosidase-from-higher-termite-microcerotermes-annandalei
#1
Siriphan Arthornthurasuk, Wantha Jenkhetkan, Eukote Suwan, Daranee Chokchaichamnankit, Chantragan Srisomsap, Pakorn Wattana-Amorn, Jisnuson Svasti, Prachumporn T Kongsaeree
A novel β-glucosidase from higher termite Microcerotermes annandalei (MaBG) was obtained via a screening method targeting β-glucosidases with increased activities in the presence of glucose. The purified natural MaBG showed a subunit molecular weight of 55 kDa and existed in a native form as a dimer without any glycosylation. Gene-specific primers designed from its partial amino acid sequences were used to amplify the corresponding 1,419-bp coding sequence of MaBG which encodes a 472-amino acid glycoside hydrolase family 1 (GH1) β-glucosidase...
May 19, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29779127/physiological-and-transcriptomic-analysis-of-a-chronologically-long-lived-saccharomyces-cerevisiae-strain-obtained-by-evolutionary-engineering
#2
Mevlüt Arslan, Can Holyavkin, Halil İbrahim Kısakesen, Alican Topaloğlu, Yusuf Sürmeli, Zeynep Petek Çakar
High-throughput aging studies with yeast as a model organism involve transposon-mutagenesis and yeast knockout collection, which have been pivotal strategies for understanding the complex cellular aging process. In this study, a chronologically long-lived Saccharomyces cerevisiae mutant was successfully obtained by using another high-throughput approach, evolutionary engineering, based on systematic selection in successive batch cultures under gradually increasing levels of caloric restriction. Detailed comparative physiological and transcriptomic analyses of the chronologically long-lived mutant and the reference strain revealed enhanced levels of respiratory metabolism, upregulation of genes related to carbohydrate metabolic processes, glycogen-trehalose pathways, stress response, and repression of protein synthesis-related genes in the long-lived mutant SRM11, already in the absence of caloric restriction...
May 19, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29779110/translocation-of-oligonucleotide-oligosaccharide-complexes-into-cells-of-the-brain
#3
N N Dygalo, T S Kalinina, D A Lanshakov
Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells...
March 2018: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29779062/synthesis-and-characterization-of-silibinin-phenanthroline-neocuproine-copper-ii-complexes-for-augmenting-bone-tissue-regeneration-an-in-vitro-analysis
#4
Subramaniyam Rajalakshmi, Selvaraj Vimalraj, Sekaran Saravanan, Desingh Raj Preeth, Manickaraj Shairam, Dhanasekaran Anuradha
In the recent decades, flavonoid metal complexes have been widely investigated for their multifaceted role in enabling osteoblast differentiation and bone formation. Silibinin complexed with copper(II) ion has been synthesized along with two mixed ligand complexes, namely copper(II) silibinin-phenanthroline and neocuproine as co-ligands, and their positive role in promoting neovacularization and osteoblast differentiation was investigated. Silibinin mono complex [Cu(sil)(H2 O)2 ] and [Cu(sil)(phen)] showed similar UV-visible absorption in the region of 315 and 222 nm, whereas Cu(silibinin)(neocuproine) [Cu(sil)(neo)] showed a blueshift in the 320 nm transition...
May 19, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/29779043/glis1-3-transcription-factors-critical-roles-in-the-regulation-of-multiple-physiological-processes-and-diseases
#5
REVIEW
Anton M Jetten
Krüppel-like zinc finger proteins form one of the largest families of transcription factors. They function as key regulators of embryonic development and a wide range of other physiological processes, and are implicated in a variety of pathologies. GLI-similar 1-3 (GLIS1-3) constitute a subfamily of Krüppel-like zinc finger proteins that act either as activators or repressors of gene transcription. GLIS3 plays a critical role in the regulation of multiple biological processes and is a key regulator of pancreatic β cell generation and maturation, insulin gene expression, thyroid hormone biosynthesis, spermatogenesis, and the maintenance of normal kidney functions...
May 19, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29779041/the-potential-importance-of-myeloid-derived-suppressor-cells-mdscs-in-the-pathogenesis-of-alzheimer-s-disease
#6
REVIEW
Antero Salminen, Kai Kaarniranta, Anu Kauppinen
The exact cause of Alzheimer's disease (AD) is still unknown, but the deposition of amyloid-β (Aβ) plaques and chronic inflammation indicates that immune disturbances are involved in AD pathogenesis. Recent genetic studies have revealed that many candidate genes are expressed in both microglia and myeloid cells which infiltrate into the AD brains. Invading myeloid cells controls the functions of resident microglia in pathological conditions, such as AD pathology. AD is a neurologic disease with inflammatory component where the immune system is not able to eliminate the perpetrator, while, concurrently, it should prevent neuronal injuries induced by inflammation...
May 19, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29778843/a-synbiotic-improves-the-immunity-of-white-shrimp-litopenaeus-vannamei-metabolomic-analyses-reveal-compelling-evidence
#7
Truong-Giang Huynh, Ann-Chang Cheng, Chia-Chun Chi, Kuo-Hsun Chiu, Chun-Hung Liu
In this study, we examined the synergistic effects of a diet-administered synbiotic comprising galactooligosaccharide (GOS) and the probiotic Lactobacillus plantarum 7-40 on immune responses, immune-related gene expressions, and disease resistance to Vibrio alginolyticus in white shrimp Litopenaeus vannamei. To unravel the regulatory role of the synbiotic in activating the immune system of shrimp, 1 H nuclear magnetic resonance (NMR)-based metabolomic analyses were used to investigate hepatopancreas metabolites, then significantly altered metabolites were confirmed in both the hepatopancreas and plasma by reverse-phase high-performance liquid chromatography (RP-HPLC) and spectrophotometric analyses...
May 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29778839/spermidine-mediated-hydrogen-peroxide-signaling-enhances-the-antioxidant-capacity-of-salt-stressed-cucumber-roots
#8
Jianqiang Wu, Sheng Shu, Chengcheng Li, Jin Sun, Shirong Guo
Hydrogen peroxide (H2 O2 ) is a key signaling molecule that mediates a variety of physiological processes and defense responses against abiotic stress in higher plants. In this study, our aims are to clarify the role of H2 O2 accumulation induced by the exogenous application of spermidine (Spd) to cucumber (Cucumis sativus) seedlings in regulating the antioxidant capacity of roots under salt stress. The results showed that Spd caused a significant increase in endogenous polyamines and H2 O2 levels, and peaked at 2 h after salt stress...
May 5, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29778836/interrogation-of-mammalian-protein-complex-structure-function-and-membership-using-genome-scale-fitness-screens
#9
Joshua Pan, Robin M Meyers, Brittany C Michel, Nazar Mashtalir, Ann E Sizemore, Jonathan N Wells, Seth H Cassel, Francisca Vazquez, Barbara A Weir, William C Hahn, Joseph A Marsh, Aviad Tsherniak, Cigall Kadoch
Protein complexes are assemblies of subunits that have co-evolved to execute one or many coordinated functions in the cellular environment. Functional annotation of mammalian protein complexes is critical to understanding biological processes, as well as disease mechanisms. Here, we used genetic co-essentiality derived from genome-scale RNAi- and CRISPR-Cas9-based fitness screens performed across hundreds of human cancer cell lines to assign measures of functional similarity. From these measures, we systematically built and characterized functional similarity networks that recapitulate known structural and functional features of well-studied protein complexes and resolve novel functional modules within complexes lacking structural resolution, such as the mammalian SWI/SNF complex...
May 14, 2018: Cell Systems
https://www.readbyqxmd.com/read/29778713/concluding-commentary-on-current-trends-to-enhance-the-clinical-safety-of-pediatric-transfusion-focusing-on-prevention-of-untoward-complications-of-hsc-transplantation-newer-strategies-for-improving-the-standards-of-safety-quality-of-stem-cells-expansion-for
#10
REVIEW
Irena Sniecinski, Jerard Seghatchian
Clinical practice and related diagnostic, development and research [DDR] strategies in pediatric transfusion and transplantation cover a broad range of multidisciplinary studies, performed by many professionals involved in this most challenging clinical field [1]. This commentary on the current position and future perspectives in pediatric transfusion field is aimed to highlight major unresolved transfusion complications in pediatric patients, namely red blood cell and platelet alloimmunisation, and new ones such as nosocomial infection, thrombosis and multi-organ failure...
May 16, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29778664/gestational-diabetes-mellitus-modulates-cholesterol-homeostasis-in-human-fetoplacental-endothelium
#11
Yidan Sun, Susanne Kopp, Jasmin Strutz, Chaitanya Chakravarthi Gali, Martina Zandl-Lang, Elham Fanaee-Danesh, Andrijana Kirsch, Silvija Cvitic, Saša Frank, Richard Saffery, Ingemar Björkhem, Gernot Desoye, Christian Wadsack, Ute Panzenboeck
Gestational diabetes mellitus (GDM) is associated with excessive oxidative stress which may affect placental vascular function. Cholesterol homeostasis is crucial for maintaining fetoplacental endothelial function. We aimed to investigate whether and how GDM affects cholesterol metabolism in human fetoplacental endothelial cells (HPEC). HPEC were isolated from fetal term placental arterial vessels of GDM or control subjects. Cellular reactive oxygen species (ROS) were detected by H2 DCFDA fluorescent dye. Oxysterols were quantified by gas chromatography-mass spectrometry analysis...
May 17, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29778623/hypothesis-driven-versus-hypothesis-free-approaches-to-the-identification-of-genes-for-cleft-lip-and-palate
#12
Alexandre R Vieira
Much progress has been made in understanding the genetic contributions to isolated forms of cleft lip and palate, however this effort is yet to be translated to enhanced genetic counseling or prevention strategies. Interest in the topic was renewed with the ability to perform genome-wide genotyping and after a few of those relatively larger scale studies, many are considering more targeted efforts and to focus on specific genes and/or loci. Here we discuss this crossroad and suggest targeted approaches and rethought clinical descriptions are a better way to move the field forward...
May 16, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29778523/local-immunomodulation-with-anti-inflammatory-cytokine-encoding-lentivirus-enhances-functional-recovery-after-spinal-cord-injury
#13
Jonghyuck Park, Joseph T Decker, Daniel J Margul, Dominique R Smith, Brian J Cummings, Aileen J Anderson, Lonnie D Shea
Trauma to the spinal cord and associated secondary inflammation can lead to permanent loss of sensory and motor function below the injury level, with the resulting environment serving as a barrier that limits regeneration. In this study, we investigate the localized expression of anti-inflammatory cytokines IL-10 and IL-4 via lentiviral transduction in multichannel bridges. Porous multichannel bridges provide physical guidance for axonal outgrowth with the cytokines hypothesized to modulate the neuroinflammatory microenvironment and enhance axonal regeneration...
April 27, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29778441/hypoxic-postconditioning-enhances-functional-recovery-following-endothelin-1-induced-middle-cerebral-artery-occlusion-in-conscious-rats
#14
Hong L Nguyen, Alexander M Ruhoff, Thomas Fath, Nicole M Jones
Stroke is a leading cause of death and a major contributor to neurological disability in adults. Tissue plasminogen activator is the only approved treatment. However, due to its narrow therapeutic window, <5% of patients receive treatment. Recently, hypoxic postconditioning (HPC) was shown to reduce stroke induced-injury in mice, but the mechanisms and functional outcomes are still unknown. In the current study, male Sprague Dawley rats were subjected to endothelin-1 induced stroke. HPC (8% O2 , 1 h/d for 5d) or normoxia treatments were started 24 h after stroke...
May 17, 2018: Experimental Neurology
https://www.readbyqxmd.com/read/29778389/resveratrol-improves-in-vitro-maturation-of-oocytes-in-aged-mice-and-humans
#15
Mei-Ju Liu, Ai-Gang Sun, Shi-Gang Zhao, Hui Liu, Shui-Ying Ma, Mei Li, Ying-Xue Huai, Han Zhao, Hong-Bin Liu
OBJECTIVE: To evaluate the effects of resveratrol on oocyte maturation in aged mice and humans. DESIGN: Experimental laboratory study. SETTING: University-based reproductive medicine center. PATIENT(S): A total of 64 women 38-45 years of age undergoing intracytoplasmic sperm injection (ICSI) and 48-52-week-old female C57BL/6J mice. INTERVENTION(S): In vitro culture in the presence of three different concentrations of resveratrol (0...
May 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29778190/emergence-of-the-colistin-resistance-gene-mcr-1-and-its-variant-in-several-uncommon-species-of-enterobacteriaceae-from-commercial-poultry-farm-surrounding-environments
#16
Xiaoming Wang, Yao Wang, Yang Wang, Suxia Zhang, Zhangqi Shen, Shaolin Wang
The colistin resistance gene mcr-1 has been detected in multiple members of Enterobacteriaceae family. Here, we report the emergence of mcr-1 in Providencia alcalifaciens and a mcr-1 variant, named mcr-1.3, in Raoultella planticola. Both of the mcr-1-carrying plasmids in these two isolates belong to IncI2 type of plasmids, but they are different in sizes and genetic characteristics. We also detected the mcr-1 gene in one Enterobacter cloacae isolate, however, the mcr-1-carrying plasmid is distinct from the previous reports...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778158/community-structures-and-population-dynamics-of-candidatus-accumulibacter-in-activated-sludges-of-wastewater-treatment-plants-using-ppk1-as-phylogenetic-marker
#17
Wei Zeng, Limin Zhang, Pengchao Fan, Jingjing Guo, Yongzhen Peng
Candidatus Accumulibacter has been identified as dominant polyphosphate-accumulating organisms (PAOs) in enhanced biological phosphorus (P) removal (EBPR) from wastewater. This study revealed the relevance of community structure, abundance and seasonal population dynamics of Candidatus Accumulibacter to process operation of wastewater treatment plants (WWTPs) in China using ppk1 gene as phylogenetic marker. All sludge samples had properties of denitrifying P removal using nitrate as an electron acceptor. Accumulibacter abundance in the anaerobic-anoxic-oxic (A2 O) process was the highest (26% of total bacteria), and higher in winter than in summer with a better EBPR performance...
May 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29778095/establishing-an-innovative-carbohydrate-metabolic-pathway-for-efficient-production-of-2-keto-l-gulonic-acid-in-ketogulonicigenium-robustum-initiated-by-intronic-promoters
#18
Cai-Yun Wang, Ye Li, Zi-Wei Gao, Li-Cheng Liu, Meng-Yue Zhang, Tian-Yuan Zhang, Chun-Fu Wu, Yi-Xuan Zhang
BACKGROUND: 2-Keto-L-gulonic acid (2-KGA), the precursor of vitamin C, is currently produced by two-step fermentation. In the second step, L-sorbose is transformed into 2-KGA by the symbiosis system composed of Ketogulonicigenium vulgare and Bacillus megaterium. Due to the different nutrient requirements and the uncertain ratio of the two strains, the symbiosis system significantly limits strain improvement and fermentation optimization. RESULTS: In this study, Ketogulonicigenium robustum SPU_B003 was reported for its capability to grow well independently and to produce more 2-KGA than that of K...
May 19, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29777958/sssupramolecular-poly-acrylic-acid-f127-hydrogel-with-hydration-controlled-nitric-oxide-release-for-enhancing-wound-healing
#19
Mathilde Champeau, Valéria Póvoa, Lucas Militão, Flávia Cabrini, Guilherme F Picheth, Florian Meneau, Carlos P Jara, Eliana P de Araujo, Marcelo G de Oliveira
Topical nitric oxide (NO) delivery has been shown to accelerate wound healing. However, delivering NO to wounds at appropriate rates and doses requires new biomaterial-based strategies. Here, we describe the development of supramolecular interpolymer complex hydrogels comprising PEO-PPO-PEO (F127) micelles embedded in a poly(acrylic acid) (PAA) matrix, with S-nitrosoglutathione (GSNO) molecules dissolved in the hydrophilic domain. We show that PAA:F127/GSNO hydrogels start releasing NO upon hydration at rates controlled by their rates of water absorption...
May 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29777912/a-super-enhancer-maintains-homeostatic-expression-of-regnase-1
#20
Riyun Yang, Yuanyuan Wu, Yue Ming, Yuanpei Xu, Shouyan Wang, Jianbo Shen, Chenlu Wang, Xia Chen, Yongming Wang, Renfang Mao, Yihui Fan
Regnase-1 is not only a key component in maintaining intracellular homeostasis but also a critical negative regulator in preventing autoimmune diseases and cancer development. To keep homeostatic state, Regnase-1 has to be maintained at a desired level in multiple cell types. However, the molecular mechanism of keeping a certain transcriptional level of Reganase-1 is largely unknown. In this study, we found a super-enhancer (Reg-1-SE) around Regnase-1 gene is able to control the homeostatic expression of Regnase-1...
May 16, 2018: Gene
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