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https://www.readbyqxmd.com/read/27924004/temporal-association-of-orca-lrwd1-to-late-firing-origins-during-g1-dictates-heterochromatin-replication-and-organization
#1
Yating Wang, Abid Khan, Anna B Marks, Owen K Smith, Sumanprava Giri, Yo-Chuen Lin, Rachel Creager, David M MacAlpine, Kannanganattu V Prasanth, Mirit I Aladjem, Supriya G Prasanth
DNA replication requires the recruitment of a pre-replication complex facilitated by Origin Recognition Complex (ORC) onto the chromatin during G1 phase of the cell cycle. The ORC-associated protein (ORCA/LRWD1) stabilizes ORC on chromatin. Here, we evaluated the genome-wide distribution of ORCA using ChIP-seq during specific time points of G1. ORCA binding sites on the G1 chromatin are dynamic and temporally regulated. ORCA association to specific genomic sites decreases as the cells progressed towards S-phase...
December 6, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27914447/acoustical-gas-leak-detection-in-the-presence-of-multiple-reflections-dispersion-and-uncorrelated-noise-using-optimized-residual-complexity
#2
A M Ahmadi, A Amjadi, A R Bahrampour, H Ravanbod, S Tofighi
Precise acoustical leak detection calls for robust time-delay estimates, which minimize the probability of false alarms in the face of dispersive propagation, multiple reflections, and uncorrelated background noise. Providing evidence that higher order modes and multi-reflected signals behave like sets of correlated noise, this work uses a regression model to optimize residual complexity in the presence of both correlated and uncorrelated noise. This optimized residual complexity (ORC) is highly robust since it takes into account both the level and complexity of noise...
September 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27906128/two-subunits-of-human-orc-are-dispensable-for-dna-replication-and-proliferation
#3
Etsuko Shibata, Manjari Kiran, Yoshiyuki Shibata, Samarendra Singh, Shashi Kiran, Anindya Dutta
The six-subunit Origin Recognition Complex (ORC) is believed to be an essential eukaryotic ATPase that binds to origins of replication as a ring-shaped heterohexamer to load MCM2-7 and initiate DNA replication. We have discovered that human cell lines in culture proliferate with intact chromosomal origins of replication after disruption of both alleles of ORC2 or of the ATPase subunit, ORC1. The ORC1 or ORC2-depleted cells replicate with decreased chromatin loading of MCM2-7 and become critically dependent on another ATPase, CDC6, for survival and DNA replication...
December 1, 2016: ELife
https://www.readbyqxmd.com/read/27900466/overview-of-the-impact-of-depression-and-anxiety-in-chronic-obstructive-pulmonary-disease
#4
Josep Montserrat-Capdevila, Pere Godoy, Josep Ramon Marsal, Ferran Barbé, Josep Pifarré, Miquel Alsedà, Marta Ortega
BACKGROUND: Anxiety and depression are common entities in patients diagnosed with COPD. However, the impact that they have on the exacerbation of illness is scarcely studied. OBJECTIVE: To determine if the presence of anxiety and depression is associated with a greater risk of frequent exacerbation (≥2 per year) in patients diagnosed with COPD. PATIENTS AND METHODS: A cohort study that analysed frequent exacerbation and associated factors in 512 patients monitored during 2 years...
November 29, 2016: Lung
https://www.readbyqxmd.com/read/27864185/the-pediatric-international-knee-documentation-committee-pedi-ikdc-subjective-knee-evaluation-form-normative-data
#5
Adam Y Nasreddine, Patricia L Connell, Leslie A Kalish, Susan Nelson, Maura D Iversen, Allen F Anderson, Mininder S Kocher
BACKGROUND: Children are participating in sports at an increasingly younger age, which has contributed to an increased incidence of knee injuries among this population. Because of increased interest in the application of patient-reported outcome measures in orthopaedic surgery to evaluate treatment results, numerous knee outcome scores, including the International Knee Documentation Committee Subjective Form (IKDC), have been used to evaluate the knee. Although the IKDC has been validated only in adults, it is also being used for children because of a lack of appropriate outcome scores in the pediatric population...
November 18, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27862828/introduction-of-robotically-assisted-radical-cystectomy-within-an-established-enhanced-recovery-programme
#6
Catherine Miller, Nicholas J Campain, Rachel Dbeis, Mark Daugherty, Nicholas Batchelor, Elizabeth Waine, John S McGrath
OBJECTIVES: In recent years, there has been rapid adoption of robotically-assisted surgery (RAS) for the treatment of pelvic urological cancers. This is particularly true for radical prostatectomy (RP) where robotically-assisted laparoscopic prostatectomy (RALP) has become the predominant surgical approach across England. Despite this, less than 15% of patients undergoing radical cystectomy (RC) in England in 2014 underwent a robotically-assisted radical cystectomy (RARC). However, as expertise in RAS spreads, an increasing number of cancer centres are now adopting this approach for patients undergoing RC...
November 15, 2016: BJU International
https://www.readbyqxmd.com/read/27836746/trf2-recruits-orc-through-trfh-domain-dimerization
#7
Mitsunori Higa, Tatsunori Kushiyama, Seiichiro Kurashige, Daisuke Kohmon, Kouki Enokitani, Satoko Iwahori, Nozomi Sugimoto, Kazumasa Yoshida, Masatoshi Fujita
Telomeres are specialized chromatin structures that prevent the degradation and instability of the ends of linear chromosomes. While telomerase maintains long stretches of the telomeric repeat, the majority of telomeric DNA is duplicated by conventional DNA replication. A fundamental step in eukaryotic DNA replication involves chromatin binding of the origin recognition complex (ORC). In human cells, telomeric repeat binding factor 2 (TRF2) is thought to play a role in the recruitment of ORC onto telomeres...
January 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27829632/quantitative-analysis-of-trace-elements-in-silicate-glass-sample-by-la-icp-qms-qms-with-an-orc-silicon-as-the-matrix-of-calibrating-solutions-and-the-internal-standard-for-measurement
#8
Yanbei Zhu
A strategy was presented for quantitative analysis of trace elements in a glass sample by laser ablation inductively coupled plasma tandem quadrupole mass spectrometry (LA-ICP-QMS/QMS) with an octapole reaction cell (ORC). Silicon in the glass was used as the internal standard as well as the matrix for making the calibrating solutions. Sample aerosols generated by the laser ablation system were introduced into the dual pass spray chamber through the make-up gas port. Calibrating solutions were nebulized into the spray chamber using a microflow nebulizer...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27822014/hyaluronic-acid-and-oxidized-regenerated-cellulose-prevent-adhesion-reformation-after-adhesiolysis-in-rat-models
#9
Yan Zhang, Qin Liu, Ning Yang, Xuegang Zhang
Postsurgical adhesion formation is the most common complication in abdominal and pelvic surgery. Adhesiolysis is the most commonly applied treatment for adhesion formation but is often followed by adhesion reformation. Therefore, an efficient strategy should be adopted to solve these problems. This study aimed to explore whether hyaluronic acid and oxidized regenerated cellulose (ORC) could prevent adhesion formation and reformation. Thirty female Sprague Dawley rats were randomly divided into three groups (n=10 each) and subjected to different treatments during the first and second surgery...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27820855/robotic-assisted-radical-cystectomy-with-extracorporeal-urinary-diversion-does-not-show-a-benefit-over-open-radical-cystectomy-a-systematic-review-and-meta-analysis-of-randomised-controlled-trials
#10
Wei Shen Tan, Pramit Khetrapal, Wei Phin Tan, Simon Rodney, Marisa Chau, John D Kelly
BACKGROUND: The number of robotic assisted radical cystectomy (RARC) procedures is increasing despite the lack of Level I evidence showing any advantages over open radical cystectomy (ORC). However, several systematic reviews with meta-analyses including non-randomised studies, suggest an overall benefit for RARC compared to ORC. We performed a systematic review with meta-analysis of randomised controlled trials (RCTs) to evaluate the perioperative morbidity and efficacy of RARC compared to ORC in patients with bladder cancer...
2016: PloS One
https://www.readbyqxmd.com/read/27766649/functions-of-corazonin-and-histamine-in-light-entrainment-of-the-circadian-pacemaker-in-the-madeira-cockroach-rhyparobia-maderae
#11
Andreas Arendt, El-Sayed Baz, Monika Stengl
The circadian pacemaker of the Madeira cockroach, Rhyparobia (Leucophaea) maderae, is located in the accessory medulla (AME). Ipsi- and contralateral histaminergic compound eyes are required for photic entrainment. Light pulses delay locomotor activity rhythm during the early night and advance it during the late night. Thus, different neuronal pathways might relay either light-dependent delays or advances to the clock. Injections of neuroactive substances combined with running-wheel assays suggested that GABA, pigment-dispersing factor, myoinhibitory peptides (MIPs), and orcokinins (ORCs) were part of both entrainment pathways, whereas allatotropin (AT) only delayed locomotor rhythms at the early night...
October 20, 2016: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/27744293/regulation-of-dna-replication-and-chromosomal-polyploidy-by-the-mll-wdr5-rbbp5-methyltransferases
#12
Fei Lu, Xiaojun Wu, Feng Yin, Christina Chia-Fang Lee, Min Yu, Ivailo S Mihaylov, Jiekai Yu, Hong Sun, Hui Zhang
DNA replication licensing occurs on chromatin, but how the chromatin template is regulated for replication remains mostly unclear. Here, we have analyzed the requirement of histone methyltransferases for a specific type of replication: the DNA re-replication induced by the downregulation of either Geminin, an inhibitor of replication licensing protein CDT1, or the CRL4CDT2 ubiquitin E3 ligase. We found that siRNA-mediated reduction of essential components of the MLL-WDR5-RBBP5 methyltransferase complexes including WDR5 or RBBP5, which transfer methyl groups to histone H3 at K4 (H3K4), suppressed DNA re-replication and chromosomal polyploidy...
October 15, 2016: Biology Open
https://www.readbyqxmd.com/read/27731435/processing-characterization-and-hemostatic-mechanism-of-a-ultraporous-collagen-orc-biodegradable-composite-with-excellent-biological-effectiveness
#13
Weilu Cheng, Hui Li, Xiongfei Zheng, Lei Chen, Feng Cheng, Jinmei He, Ke Liu, Panpan Cai, Xiaofeng Wang, Yudong Huang
Collagen, one of the most biocompatible materials in nature, is widely used in wound healing and organ repair. However, the limited mechanical strength and biological effectiveness of collagen restrain its application as a hemostasis and filling material in medicine. To overcome these limitations, ultraporous collagen/oxidized regenerated cellulose (Col/ORC) composites were prepared. The results showed that the Col-0.25%ORC composite had optimal wettability, porosity, and water absorption. An MTT assay proved that the Col and Col/ORC composites possessed no cytotoxicity in living cells...
October 12, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27702582/geminin-a-multi-task-protein-involved-in-cancer-pathophysiology-and-developmental-process-a-review
#14
Prem Prakash Kushwaha, Krishna Chaitanya Rapalli, Shashank Kumar
DNA replicates in a timely manner with each cell division. Multiple proteins and factors are involved in the initiation of DNA replication including a dynamic interaction between Cdc10-dependent transcript (Cdt1) and Geminin (GMNN). A conformational change between GMNN-Cdt1 heterotrimer and heterohexamer complex is responsible for licensing or inhibition of the DNA replication. This molecular switch ensures a faithful DNA replication during each S phase of cell cycle. GMNN inhibits Cdt1-mediated minichromosome maintenance helicases (MCM) loading onto the chromatin-bound origin recognition complex (ORC) which results in the inhibition of pre-replication complex assembly...
October 1, 2016: Biochimie
https://www.readbyqxmd.com/read/27701460/the-replicative-consequences-of-papillomavirus-e2-protein-binding-to-the-origin-replication-factor-orc2
#15
Marsha DeSmet, Sriramana Kanginakudru, Anne Rietz, Wai-Hong Wu, Richard Roden, Elliot J Androphy
The origin recognition complex (ORC) coordinates a series of events that lead to initiation of DNA strand duplication. As a nuclear double stranded DNA plasmid, the papillomavirus (PV) genome resembles a mini-chromosome in infected cells. To initiate its replication, the viral E2 protein binds to and recruits the E1 DNA helicase at the viral origin. PV genome replication program exhibits three stages: initial amplification from a single genome upon infection to a few copies per cell, a cell cycle linked maintenance phase, and a differentiation dependent late stage where the genome is amplified to thousands of copies...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27682158/reduction-mammoplasty-using-oxidized-regenerated-cellulose-as-a-filler-in-breast-conservation-surgery
#16
Gianluca Franceschini, Alejandro Martin Sanchez, Giuseppe Visconti, Alba Di Leone, Sabatino D'Archi, Antonino Mulè, Angela Santoro, Marzia Salgarello, Riccardo Masetti
: Oncoplastic surgery of the breast has generated great excitement over the past years and has become an integrated component of the surgical treatment of breast cancer. Oncoplastic procedures (OPP) associate the best surgical oncologic principles to achieve wide tumor-free margins with the best principles of plastic surgery to optimize cosmetic outcomes. So, a number of conventional mammoplasty techniques have been adapted to allow reconstruction of resection defects with parenchymal flaps using a variety of different approaches...
2016: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/27679476/h3k9me3-demethylase-kdm4d-facilitates-the-formation-of-pre-initiative-complex-and-regulates-dna-replication
#17
Rentian Wu, Zhiquan Wang, Honglian Zhang, Haiyun Gan, Zhiguo Zhang
DNA replication is tightly regulated to occur once and only once per cell cycle. How chromatin, the physiological substrate of DNA replication machinery, regulates DNA replication remains largely unknown. Here we show that histone H3 lysine 9 demethylase Kdm4d regulates DNA replication in eukaryotic cells. Depletion of Kdm4d results in defects in DNA replication, which can be rescued by the expression of H3K9M, a histone H3 mutant transgene that reverses the effect of Kdm4d on H3K9 methylation. Kdm4d interacts with replication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative complex components (origin recognition complex [ORC] and minichromosome maintenance [MCM] complex)...
September 26, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27664079/systematic-review-and-meta-analysis-of-randomised-trials-of-perioperative-outcomes-comparing-robot-assisted-versus-open-radical-cystectomy
#18
Zhiyuan Shen, Zhongquan Sun
BACKGROUND: With the introduction of robotic surgery, whether the robot-assisted radical cystectomy (RARC) could reduce the perioperative morbidity compared with Open radical cystectomy (ORC) was unknown. METHODS: Studies reported RARC were reviewed based on all randomized controlled trials (RCTs), which focused on the efficacy of RARC versus ORC. RESULTS: Of the 201 studies from preliminary screening, four RCTs were included. By pooling these studies, there were significant differences in comparison of operative time (p = 0...
September 23, 2016: BMC Urology
https://www.readbyqxmd.com/read/27661614/development-and-evaluation-of-real-time-rt-pcr-assays-for-detection-and-typing-of-bluetongue-virus
#19
Sushila Maan, Narender Singh Maan, Manjunatha N Belaganahalli, Abraham C Potgieter, Vinay Kumar, Kanisht Batra, Isabel M Wright, Peter D Kirkland, Peter P C Mertens
Bluetongue virus is the type species of the genus Orbivirus, family Reoviridae. Bluetongue viruses (BTV) are transmitted between their vertebrate hosts primarily by biting midges (Culicoides spp.) in which they also replicate. Consequently BTV distribution is dependent on the activity, geographic distribution, and seasonal abundance of Culicoides spp. The virus can also be transmitted vertically in vertebrate hosts, and some strains/serotypes can be transmitted horizontally in the absence of insect vectors...
2016: PloS One
https://www.readbyqxmd.com/read/27635237/how-mcm-loading-and-spreading-specify-eukaryotic-dna-replication-initiation-sites
#20
REVIEW
Olivier Hyrien
DNA replication origins strikingly differ between eukaryotic species and cell types. Origins are localized and can be highly efficient in budding yeast, are randomly located in early fly and frog embryos, which do not transcribe their genomes, and are clustered in broad (10-100 kb) non-transcribed zones, frequently abutting transcribed genes, in mammalian cells. Nonetheless, in all cases, origins are established during the G1-phase of the cell cycle by the loading of double hexamers of the Mcm 2-7 proteins (MCM DHs), the core of the replicative helicase...
2016: F1000Research
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