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https://www.readbyqxmd.com/read/28330309/molecular-characterization-of-phytoplasmas-of-clover-proliferation-group-associated-with-three-ornamental-plant-species-in-india
#1
Ekta Khasa, Gopala, Aido Taloh, T Prabha, Madhupriya, G P Rao
Suspected phytoplasma symptoms of little leaf, yellowing, chlorosis, phyllody, witches' broom, and stunting were observed on ten different ornamental plant species at New Delhi, Andhra Pradesh, Haryana, Bengaluru, and Pune, India, during March to July 2016. To investigate the possibility of phytoplasma etiology, PCR assays were performed using universal primer pairs (P1/P7 followed by 3Far/3Rev) specific to the phytoplasma 16Sr RNA gene. First round PCR amplification with primer pair P1/P7 did not yield expected 1...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330298/biodegradation-of-used-engine-oil-by-novel-strains-of-ochrobactrum-anthropi-hm-1-and-citrobacter-freundii-hm-2-isolated-from-oil-contaminated-soil
#2
Haytham M M Ibrahim
Used engine oil (UEO) constitutes a serious environmental problem due to the difficulty of disposal off or reuse. Ten bacterial strains with biodegradation potential were isolated from UEO-contaminated soil sample using enrichment technique. Two strains which exhibited the highest degradation %, 51 ± 1.2 and 48 ± 1.5, respectively, were selected. Based on the morphological, biochemical characteristics and 16S rRNA sequence analysis, they were identified as Ochrobactrum anthropi HM-1 (accession no: KR360745) and Citrobacter freundii HM-2 (accession no: KR360746)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330283/multiplex-pcr-for-simultaneous-identification-of-e-coli-o157-h7-salmonella-spp-and-l-monocytogenes-in-food
#3
Thuy Trang Nguyen, Vo Van Giau, Tuong Kha Vo
The rapid detection of pathogens in food is becoming increasingly critical for ensuring the safety of consumers, since the majority of food-borne illnesses and deaths are caused by pathogenic bacteria. Hence, rapid, sensitive, inexpensive and convenient approaches to detect food-borne pathogenic bacteria is essential in controlling food safety. In this study, a multiplex PCR assay for the rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes was established...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330269/identification-of-the-traditional-and-non-traditional-sulfate-reducing-bacteria-associated-with-corroded-ship-hull
#4
Kiana Alasvand Zarasvand, V Ravishankar Rai
Pitting corrosion due to microbial activity is the most severe type of corrosion that occurs in ship hull. Since biogenic sulfide produced by sulfate-reducing bacteria (SRB) is involved in the acceleration of pitting corrosion of marine vessels, so it is important to collect information about SRB community involved in maritime vessel failure. We investigated the SRB community on corroded hull portion of the ship. With the use of common cultural method and 16S rDNA sequencing, ten bacteria with sulfate reduction ability were isolated and identified...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330243/micropropagation-and-validation-of-genetic-and-biochemical-fidelity-among-regenerants-of-nothapodytes-nimmoniana-graham-mabb-employing-issr-markers-and-hplc
#5
Lokesh Prakash, Sushil Kumar Middha, Sudipta Kumar Mohanty, Mallappa Kumara Swamy
An in vitro protocol has been established for clonal propagation of Nothapodytes nimmoniana which is an important source of Camptothecin (CPT). Elite source was identified based on the chemical potency to accumulate the optimum level of CPT. Different types and concentrations of plant growth regulators were used to study their effect on inducing multiple shoots from the explants regenerated from embryos of N. nimmoniana. Of these, a combination of N6-benzyladenine (0.2 mg L(-1)) and Indole-3-butyric acid (IBA) (0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330135/confamiliar-transferability-of-simple-sequence-repeat-ssr-markers-from-cotton-gossypium-hirsutum-l-and-jute-corchorus-olitorius-l-to-twenty-two-malvaceous-species
#6
Pratik Satya, Pramod Kumar Paswan, Swagata Ghosh, Snehalata Majumdar, Nasim Ali
Cross-species transferability is a quick and economic method to enrich SSR database, particularly for minor crops where little genomic information is available. However, transferability of SSR markers varies greatly between species, genera and families of plant species. We assessed confamiliar transferability of SSR markers from cotton (Gossypium hirsutum) and jute (Corchorus olitorius) to 22 species distributed in different taxonomic groups of Malvaceae. All the species selected were potential industrial crop species having little or no genomic resources or SSR database...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330127/diversity-of-toxic-and-phytopathogenic-fusarium-species-occurring-on-cereals-grown-in-karnataka-state-india
#7
H Nagaraja, G Chennappa, K Poorna Chandra Rao, G Mahadev Prasad, M Y Sreenivasa
A total of 198 cereal samples (53 maize, 54 sorghum, 37 paddy and 54 wheat) were collected from 11 districts of Karnataka to understand the percent infection (PI), relative density (RD) and their frequency (Fr) caused by Fusarium spp. All samples were screened by agar plating method using MGA 2.5 agar media and incubated at 25 ± 2 °C for 3-5 days. The study revealed the association of 10 different Fusarium species known trichothecene producers such as Fusarium acuminatum, F. avenaceum, F. crookwellense, F...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329867/effectiveness-of-a-virtual-reality-forest-on-people-with-dementia-a-mixed-methods-pilot-study
#8
Wendy Moyle, Cindy Jones, Toni Dwan, Tanya Petrovich
Purpose of the Study: To measure and describe the effectiveness of a Virtual Reality Forest (VRF) on engagement, apathy, and mood states of people with dementia, and explore the experiences of staff, people with dementia and their families. Design and Methods: A mixed-methods study conducted between February and May 2016. Ten residents with dementia, 10 family members, and 9 care staff were recruited from 2 residential aged care facilities, operated by one care provider, located in Victoria, Australia...
March 15, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329819/development-of-a-physical-environmental-observational-tool-for-dining-environments-in-long-term-care-settings
#9
Habib Chaudhury, Heather Keller, Kaylen Pfisterer, Lillian Hung
Purpose: This paper presents the first standardized physical environmental assessment tool titled Dining Environment Audit Protocol (DEAP) specifically designed for dining spaces in care homes and reports the results of its psychometric properties. Items rated include: adequacy of lighting, glare, personal control, clutter, staff supervision support, restraint use, and seating arrangement option for social interaction. Two scales summarize the prior items and rate the overall homelikeness and functionality of the space...
February 20, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329668/stevens-johnson-syndrome-and-toxic-epidermal-necrolysis-in-us-adults
#10
Derek Y Hsu
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are life-threatening disorders. The incidences, mortality and cost of SJS and TEN in US adults are not well-characterized. We analyzed data from the 2009-2012 Nationwide Inpatient Sample, containing a representative 20% sample of all hospitalizations in the US. SJS, SJS/TEN and TEN were identified by a validated algorithm using ICD-9- CM codes. The mean incidences of SJS, SJS/TEN and TEN were 9.3, 1.6, and 1.9 per million people per year, respectively...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329656/pediatric-stevens-johnson-syndrome-and-toxic-epidermal-necrolysis-in-the-us
#11
Derek Y Hsu
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are life-threatening blistering disorders. Few studies have addressed SJS/TEN in children. We sought to determine risk factors, comorbidities, inpatient burden and mortality in US children with SJS, SJS-TEN and TEN. We analyzed data from the 2009-2012 Nationwide Inpatient Sample (NIS), containing a representative 20% sample of all hospitalizations in the US. SJS, SJS-TEN and TEN were identified by a validated algorithm using ICD-9-CM codes...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329642/effect-of-antibiotic-exposure-on-stevens-johnson-syndrome-sjs-and-toxic-epidermal-necrolysis-ten
#12
Katherine J Sullivan, Robert J Valuck, Meghan N Jeffres, Heather D Anderson
BackgroundAntibiotics are commonly implicated in Stevens-Johnson syndrome (SJS), overlap syndrome (SJS-TEN), and Toxic Epidermal Necrolysis (TEN), rare disorders of skin and mucosal membranes, often the result of adverse drug reactions. The objective of this study is to examine associations between SJS, SJS-TEN, and TEN and antibiotic exposure overlapping diagnosis, and 3, 6, and 12 months before diagnosis.MethodsA case-control study was conducted using PharMetrics LifeLink® Health Plan Claims Database (2001-2013)...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329640/diagnostic-parameters-of-children-with-basal-cell-carcinoma-nevus-syndrome
#13
Fariha T Siddiqui
Basal Cell Carcinoma Nevus Syndrome (BCCNS) is a multi-system genetic disease characterized by the development of multiple basal cell carcinomas (BCCs), macrocephaly, medulloblastomas, jaw keratocysts, and coarse facial features, amongst other symptoms. The major and minor criteria for adults with BCCNS are often extrapolated for children, however, little is known about the disease presentation of children with BCCNS. Our study focused on bringing the pediatric presentation of BCCNS to light. To the best of our knowledge, we are the first to investigate BCCNS and its medical impact on children...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329636/correlation-between-cancer-incidences-and-google-searches-in-the-united-states
#14
Mackenzie R Wehner, Kevin T Nead, Eleni Linos
IntroductionDespite being highly prevalent, keratinocyte carcinomas (basal cell and squamous cell carcinomas lack nationwide registries. Internet search data has emerged as a new method to evaluate previously difficult to quantify public health outcomes and may be useful in keratinocyte carcinoma research.ObjectiveWe aimed to evaluate whether Google search density correlated with known incidences of common cancers in the United States.MethodsWe used the Center for Disease Control's National Program of Cancer Registries ageTadjusted cancer incidences (2008T2012 ...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329582/cyclosporine-in-toxic-epidermal-necrolysis-a-brief-review-of-the-emerging-therapeutic-modality
#15
Piyush Kumar, Nilay Kanti Das
Toxic epidermal necrolysis (TEN) is a severe life-threatening adverse drug reaction that predominantly involve the skin and mucous membranes, and is associated with high mortality (25-35% or even higher) and with various long term sequelae. There is no universally accepted treatment for TEN, but key elements of management include rapid diagnosis, identification and interruption of the culprit drug, evaluation of the prognosis using SCORTEN, specialized supportive care ideally in an intensive care unit, and consideration of immunomodulating agents...
October 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329549/frontal-fibrosing-alopecia-in-a-46-year-old-man
#16
Forrest White, Shields Callahan, Randie H Kim, Shane A Meehan, Jennifer Stein
Frontal fibrosing alopecia is a scarring alopecia thatis characterized by recession of the frontotemporalhairline with the frequent loss of eyebrows. Itpredominantly affects postmenopausal womenand only rarely affects men. We report the caseof a 46-year-old man with a ten-year history of anerythematous patch with perifollicular erythemaat the superior aspect of the forehead andfrontotemporal hairline. A skin biopsy specimenshowed a perivascular, lymphocytic infiltrate withperiinfundibular fibrosis. These findings establisheda diagnosis of frontal fibrosing alopecia...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329542/silicone-granulomas-with-ulcers
#17
Lisa R Rothman, Randie H Kim, Shane A Meehan, Alisa Femia
We present a 42-year-old transgender womanwith woody induration over her buttocks andlower extremities as well as persistent ulcers of thebuttocks. The lesions developed ten years prior to herpresentation and approximately five years after shereceived illegal silicone injections to her buttocks.Histopathologic examination was consistent witha silicone granuloma. Silicone granuloma is a notan uncommon side effect of silicone injections andmore often occurs when the filler is administeredby non-physician practitioners, as is the case in thispatient...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329541/cowden-syndrome-presenting-with-trichilemmomas
#18
Elise Ng, Vitaly Terushkin, Shane A Meehan, Roger Ho, Miriam Keltz Pomeranz
Cowden syndrome (CS) is a genetic cancerpredisposition syndrome that is associated withgermline mutations in the phosphate and tensinhomologue deleted on chromosome ten (PTEN)tumor suppressor gene. It is characterizedby the formation of benign and malignanttumors. Characteristic benign tumors includetrichilemmommas, acral keratoses, mucocutaneousneuromas, and oral papillomas. The most commonmalignant condition include breast, thyroid, andendometrial cancers. We present a case of a30-year-old woman with CS, who initially presentedwith trichilemmomas that were misdiagnosed ascomedonal acne...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329463/current-practices-in-the-timing-of-stage-2-palliation
#19
James M Meza, Robert D B Jaquiss, Brett R Anderson, Michael-Alice Moga, James K Kirklin, George Sarris, William G Williams, Brian W McCrindle
BACKGROUND: Mortality through single-ventricle palliation remains high and the effect of the timing of stage 2 palliation (S2P) is not well understood. We investigated current practice patterns in the timing of S2P across two professional societies and compared them to actual practice patterns from two databases of patients who underwent S2P. METHODS: A ten-question survey was distributed to the members of the Congenital Heart Surgeons' Society (CHSS) and the European Congenital Heart Surgeons' Association (ECHSA)...
March 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/28329425/perpendicular-axes-of-differentiation-generated-by-mitochondrial-introgression
#20
Hernán E Morales, Paul Sunnucks, Leo Joseph, Alexandra Pavlova
Differential introgression of mitochondrial versus nuclear DNA generates discordant patterns of geographic variation and can promote population divergence and speciation. We examined a potential case of mitochondrial introgression leading to two perpendicular axes of differentiation. The Eastern Yellow Robin Eopsaltria australis, a widespread Australian bird, shows a deep mitochondrial split that is perpendicular to north-south nuclear DNA and plumage colour differentiation. We propose a scenario to explain this pattern: (1) the two nuclear and mitochondrial genomes differentiated in concert during north-south population divergence; (2) later, their histories disconnected after two mitochondrial introgression events resulting in a deep mitochondrial split perpendicular to the nuclear DNA structure...
March 22, 2017: Molecular Ecology
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