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https://www.readbyqxmd.com/read/29684785/photoreceptor-calcium-sensor-proteins-in-detergent-resistant-membrane-rafts-are-regulated-via-binding-to-caveolin-1
#1
Vasiliy I Vladimirov, Evgeni Yu Zernii, Viktoriia E Baksheeva, Hanna Wimberg, Alexey S Kazakov, Natalya K Tikhomirova, Ekaterina L Nemashkalova, Vladimir A Mitkevich, Andrey A Zamyatnin, Valery M Lipkin, Pavel P Philippov, Sergei E Permyakov, Ivan I Senin, Karl-W Koch, Dmitry V Zinchenko
Rod cell membranes contain cholesterol-rich detergent-resistant membrane (DRM) rafts, which accumulate visual cascade proteins as well as proteins involved in regulation of phototransduction such as rhodopsin kinase and guanylate cyclases. Caveolin-1 is the major integral component of DRMs, possessing scaffolding and regulatory activities towards various signaling proteins. In this study, photoreceptor Ca2+ -binding proteins recoverin, NCS1, GCAP1, and GCAP2, belonging to neuronal calcium sensor (NCS) family, were recognized as novel caveolin-1 interacting partners...
April 14, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29684433/oxygen-titration-after-resuscitation-from-out-of-hospital-cardiac-arrest-a-multi-centre-randomised-controlled-pilot-study-the-exact-pilot-trial
#2
Janet E Bray, Cindy Hein, Karen Smith, Michael Stephenson, Hugh Grantham, Judith Finn, Dion Stub, Peter Cameron
INTRODUCTION: Recent studies suggest the administration of 100% oxygen to hyperoxic levels following return-of-spontaneous-circulation (ROSC) post-cardiac arrest may be harmful. However, the feasibility and safety of oxygen titration in the prehospital setting is unknown. We conducted a multi-centre, phase-2 study testing whether prehospital titration of oxygen results in an equivalent number of patients arriving at hospital with oxygen saturations SpO2 ≥ 94%. METHODS: We enrolled unconscious adults with: sustained ROSC; initial shockable rhythm; an advanced airway; and an SpO2 ≥ 95%...
April 20, 2018: Resuscitation
https://www.readbyqxmd.com/read/29684207/the-number-of-titrated-microrna-species-dictates-cerna-regulation
#3
Hua-Sheng Chiu, María Rodríguez Martínez, Elena V Komissarova, David Llobet-Navas, Mukesh Bansal, Evan O Paull, José Silva, Xuerui Yang, Pavel Sumazin, Andrea Califano
microRNAs (miRNAs) play key roles in cancer, but their propensity to couple their targets as competing endogenous RNAs (ceRNAs) has only recently emerged. Multiple models have studied ceRNA regulation, but these models did not account for the effects of co-regulation by miRNAs with many targets. We modeled ceRNA and simulated its effects using established parameters for miRNA/mRNA interaction kinetics while accounting for co-regulation by multiple miRNAs with many targets. Our simulations suggested that co-regulation by many miRNA species is more likely to produce physiologically relevant context-independent couplings...
April 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29683671/extra-cavity-effect-in-cyclodextrin-surfactant-complexation
#4
Carolyn Vargas, Christian Schönbeck, Ina Heimann, Sandro Keller
Cyclodextrin (CD) complexation is a convenient method to sequester surfactants in a controllable way, for example, during membrane-protein reconstitution. Interestingly, the equilibrium stability of CD/surfactant inclusion complexes increases with the length of the nonpolar surfactant chain even beyond the point where all hydrophobic contacts within the canonical CD cavity are saturated. To rationalize this observation, we have dissected the inclusion complexation equilibria of a structurally well-defined CD, that is, heptakis(2,6-di-O methyl)-CD (DIMEB), and a homologous series of surfactants, namely, n alkyl-N,N dimethyl-3 ammonio-1 propanesulfonates (SB3 x) with chain lengths ranging from x = 8 to 14...
April 23, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29683663/reactivity-and-specificity-of-rnase-t1-rnase-a-and-rnase-h-towards-oligonucleotides-of-rna-containing-8-oxo-7-8-dihydroguanosine
#5
Cassandra Herbert, Yannick Kokouvi Dzowo, Anthony Urban, Courtney N Kiggins, Marino J E Resendiz
Understanding how oxidatively damaged RNA interacts with ribonucleases is of importance due to its proposed role in the development/progression of disease. Thus, understanding structural aspects of RNA containing lesions generated under oxidative stress, as well as its interactions with other biopolymers is fundamental. We explored the reactivity of RNase A, RNase T1, and RNase H towards oligonucleotides of RNA containing 8-oxo-7,8-dihydroguanosine (8oxoG). This is the first example that addresses this relationship and will be useful towards understanding 1) how these RNases can be used to characterize the structural impact that this lesion has on RNA; and 2) how oxidatively modified RNA may be handled intracellularly...
April 23, 2018: Biochemistry
https://www.readbyqxmd.com/read/29683539/supramolecular-copolymerization-via-sequence-reorganization-of-supramolecular-homopolymer
#6
Kouhei Nadamoto, Kei Maruyama, Naoka Fujii, Toshiaki Ikeda, Shin-Ichi Kihara, Takeharu Haino
The homopolymeric sequence formed by the head-to-head association of tetrakisporphyrin 1 is completely dissociated via the competitive association of the ditopic guest G2 to result in the supramolecular copolymer poly-1*G2 with an alternatingly repeating host-guest sequence. The 1:1 stoichiometry of 1 and G2 is confirmed by a Job plot using UV/vis titration and diffusion-ordered NMR spectroscopy (DOSY). The solution viscometry for poly-1 and poly-1*G2 suggests that the supramolecular chain of poly-1 behaves like a rod, whereas the supramolecular copolymer chain of poly-1*G2 behaves like a swelled fat chain, which is entangled in the semi-dilute regime...
April 23, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29683535/responsive-self-assembly-of-supramolecular-hydrogel-based-on-zwitterionic-liquid-asymmetric-gemini-guest
#7
Aoli Wu, Panpan Sun, Na Sun, Liqiang Zheng
Low-molecular-weight supramolecular hydrogel was fabricated based on host-guest interaction between β-cyclodextrin (β-CD) and asymmetric Gemini zwitterionic liquid (ZIL) which contains azobenzene (Azo) and bis(trifluoromethanesulfonyl)imide (TFSI-). Reversible sol-gel phase transition could be triggered by light and temperature. The binding stoichiometry, main non-covalent interactions in hydrogel, photo and thermal responsive mechanisms, and inclusion complex mode were studied deeply by NMR spectroscopy, UV-vis spectroscopy, isothermal titration calorimetry (ITC) and control experiments...
April 23, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29683007/perioperative-noninvasive-cardiac-output-monitoring-in-parturients-undergoing-cesarean-delivery-with-spinal-anesthesia-and-prophylactic-phenylephrine-drip-a-prospective-observational-cohort-study
#8
Sharon Orbach-Zinger, Ilya Bizman, Shlomo Firman, Shaul Lev, Roi Gat, Eran Ashwal, Mordehay Vaturi, Eitan Razinski, Atara Davis, Anat Shmueli, Leonid A Eidelman
INTRODUCTION: Spinal anesthesia for cesarean delivery is associated with high incidence of hypotension and is most often prevented by a prophylactic phenylephrine infusion (PPI). In this study, we aimed to identify maternal hemodynamic changes both intraoperatively and postoperatively with the use of the NICaS noninvasive cardiac output monitor in healthy singleton parturients undergoing cesarean delivery (CD) with spinal anesthesia and PPI. METHODS: Healthy term women undergoing spinal anesthesia for singleton CD were enrolled...
April 22, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29682277/the-allergic-rhinitis-clinical-investigator-collaborative-ar-cic-verification-of-nasal-allergen-challenge-procedures-in-a-study-utilizing-an-investigational-immunotherapy-for-cat-allergy
#9
Helen Neighbour, Mena Soliman, Lisa M Steacy, Pascal Hickey, Beth Forbes, Mark Larché, Anne K Ellis
Background: The Allergic Rhinitis Clinical Investigator Collaborative (AR-CIC) is a network of experienced Allergic Rhinitis (AR) researchers developing better research tools based on the nasal allergen challenge (NAC). A key objective of such is the ability to detect efficacy in a small population. AR-CIC sought to test its NAC protocol as a secondary objective in two small mechanistic research trials of a novel form of immunotherapy [Cat Peptide Antigen Desensitisation (Cat-PAD)] for which efficacy had previously been demonstrated...
2018: Clinical and Translational Allergy
https://www.readbyqxmd.com/read/29679964/chemical-waves-in-the-o-2-h-2-reaction-on-a-rh-111-surface-alloyed-with-nickel-ii-photoelectron-spectroscopy-and-microscopy
#10
Tim Smolinsky, Mathias Homann, Bernhard von Boehn, Luca Gregoratti, Matteo Amati, Mohamed Al-Hada, Hikmet Sezen, Ronald Imbihl
Chemical waves in the H2 + O2 reaction on a Rh(111) surface alloyed with Ni [ΘNi < 1.5 monolayers (ML)] have been investigated in the 10-7 and 10-6 mbar range at T = 773 K using scanning photoelectron microscopy and x-ray photoelectron spectroscopy as in situ methods. The local intensity variations of the O 1s and the Ni 2p signal display an anticorrelated behavior. The coincidence of a high oxygen signal with a low Ni 2p intensity, which seemingly contradicts the chemical attraction between O and Ni, has been explained with a phase separation of the oxygen covered Rh(111)/Ni surface into a 3D-Ni oxide and into a Ni poor metallic phase...
April 21, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29679752/thermodynamic-compatibility-between-cyclodextrin-supramolecular-complexes-and-surfactant
#11
Larissa H R Meira, Guilherme A B Soares, Homero I M Bonomini, Juliana Fedoce Lopes, Frederico B De Sousa
Supramolecular structures based on cyclodextrins have been extensively used for drug delivery systems over decades. However, combining host and guest molecules in a pharmaceutical formulation is not a trivial process, being one of the majors concern the inclusion complex compatibility with other excipients presented in the final formulation. Herein, experimental and theoretical calculations were used to investigate the competition of sodium dodecyl sulfate (SDS) with atenolol (ATE) or losartan (LOS), antihypertensive drugs widely used in the treatment of hypertension...
April 18, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29679696/effect-of-neuromonitor-guided-titrated-care-on-brain-tissue-hypoxia-after-opioid-overdose-cardiac-arrest
#12
Jonathan Elmer, Katharyn L Flickinger, Maighdlin W Anderson, Allison Koller, Matthew Sundermann, Cameron Dezfulian, David O Okonkwo, Lori A Shutter, David Salcido, Clifton W Callaway, James J Menegazzi
INTRODUCTION: Brain tissue hypoxia may contribute to preventable secondary brain injury after cardiac arrest. We developed a porcine model of opioid overdose cardiac arrest and post-arrest care including invasive, multimodal neurological monitoring of regional brain physiology. We hypothesized brain tissue hypoxia is common with usual post-arrest care and can be prevented by modifying mean arterial pressure (MAP) and arterial oxygen concentration (PaO2 ). METHODS: We induced opioid overdose and cardiac arrest in sixteen swine, attempted resuscitation after 9 minutes of apnea, and randomized resuscitated animals to three alternating 6-hour blocks of standard or titrated care...
April 18, 2018: Resuscitation
https://www.readbyqxmd.com/read/29679154/effectiveness-of-a-mandibular-advancement-device-in-obstructive-sleep-apnea-patients-a-prospective-clinical-trial
#13
Rocío Marco Pitarch, Marina Selva García, Javier Puertas Cuesta, Jaime Marco Algarra, Enrique Fernández Julian, Antonio Fons Font
PURPOSE: This study aimed to determine the effectiveness of a mandibular advancement device in a sample of obstructive sleep apnea syndrome patients by the evaluation of respiratory and neurophysiologic parameters and clinical symptoms. Second, the influence of certain predictor factors related with the patient and the intraoral device, were considered in the final response with this treatment option. METHODS: Forty-one patients constituted the final sample. Outcomes were measured using polysomnography, Epworth sleepiness scale and an analogue visual snoring scale, before treatment and once the device was properly titrated...
April 20, 2018: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29679088/complications-and-pain-in-obstructive-sleep-apnoea-comparing-single-and-multilevel-surgery
#14
Ching Yee Chan, Hong Juan Han, Weng Kit Lye, Song Tar Toh
INTRODUCTION: This study aimed to investigate differences in the complication rate and postoperative pain score between single and multilevel surgery performed on patients with obstructive sleep apnoea. MATERIALS AND METHODS: A retrospective analysis was performed on patients with obstructive sleep apnoea who underwent surgery in a tertiary referral centre over 3 years. Patients who underwent single-level nasal, palatal or tongue surgery were compared with patients who underwent concurrent multilevel surgery of 2 or 3 levels...
March 2018: Annals of the Academy of Medicine, Singapore
https://www.readbyqxmd.com/read/29679042/effect-of-antimicrobial-peptides-from-galleria-mellonella-on-molecular-models-of-leishmania-membrane-thermotropic-and-fluorescence-anisotropy-study
#15
Isabel Andrea Patiño-Márquez, Marcela Manrique-Moreno, Edwin Patiño-González, Małgorzata Jemioła-Rzemińska, Kazimierz Strzałka
Antimicrobial peptides are molecules of natural origin, produced by organisms such as insects, which have focused attention as potential antiparasitic agents. They can cause the death of parasites such Leishmania by interacting with their membrane. In this study, additional information was obtained on how the anionic peptide 2 and cecropin D-like peptide derived from Galleria mellonella interact with liposomes that mimic the composition of the Leishmania membrane. In order to do this, lipid bilayers consisting of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, dimyristoylphosphatidylserine, and dimyristoylphosphatidylglycerol were constructed...
April 20, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29678850/cryopreservation-of-genotyped-abo-subgroup-rbcs-for-quality-assurance-of-abo-grouping-reagents
#16
Sinyoung Kim, Sungwook Song, Hyun Ok Kim
BACKGROUND: Quality assurance of newly developed or manufactured blood grouping reagents with reagent red blood cells (RBCs) is crucial in the process of product approval by governmental agency. However, RBCs with rare blood group are not easily available in the fresh state. We investigated the feasibility of cryopreserved and genotyped ABO subgroup RBC reagents for quality assurance purpose. METHODS: We obtained RBCs from 10 volunteers with ABO subgroup phenotypes...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29678734/polymeric-micelle-mediated-delivery-of-half-sandwich-ruthenium-ii-complexes-with-phosphanes-derived-from-fluoroloquinolones-for-lung-adenocarcinoma-treatment
#17
Przemysław Kołoczek, Agnieszka Skórska-Stania, Agnieszka Cierniak, Victor Sebastian, Urszula K Komarnicka, Michał Płotek, Agnieszka Kyzioł
Novel half-sandwich ruthenium(II) complexes with aminomethyl(diphenyl)phosphine derived from fluoroloquinolones (RuPCp, RuPSf, RuPLm, RuPNr) were being investigated as alternatives to well-established metal-based chemotherapeutics. All compounds were characterized by elemental analysis, selected spectroscopic methods (i.e., absorption and fluorescence spectroscopy, ESI-MS, NMR, circular dichroizm), X-ray diffractometry, ICP-MS, and electrochemical techniques. To overcome low solubility, serious side effects connected with systemic cytotoxicity of ruthenium complexes, and acquiring the resistance of cancer cells, polymeric nanoformulations based on Pluronic P-123 micelles loaded with selected Ru(II) complexes were prepared and characterized...
April 17, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29676725/bombyx-mori-nucleopolyhedrovirus-orf40-is-essential-for-budded-virus-production-and-occlusion-derived-virus-envelopment
#18
Yunwang Shen, Min Feng, Xiaofeng Wu
ORF40 (bm40) of the Bombyx mori nucleopolyhedrovirus (BmNPV) encodes a homologue of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) AC51 and is a highly conserved gene in sequenced alphabaculoviruses. To investigate the role of bm40 in the baculovirus infection cycle, a bm40 knockout BmNPV bacmid was constructed via homologous recombination in Escherichia coli. Western blotting analysis revealed that bm40 is a late gene during virus infection. Compared with wild-type and repair viruses, the knockout virus exhibited a single-cell infection phenotype...
April 19, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29676660/new-tailored-substituted%C3%A2-benzothiazole-schiff-base-cu-ii-zn-ii-antitumor-drug-entities-effect-of-substituents-on-dna-binding-profile-antimicrobial-and-cytotoxic-activity
#19
Siffeen Zehra, Mohammad Shavez Khan, Iqbal Ahmad, Farukh Arjmand
New tailored Cu(II) & Zn(II) metal-based antitumor drug entities were synthesized from substituted benzothiazole o‒vanillin Schiff base ligands. The complexes were thoroughly characterized by elemental analysis, spectroscopic studies {IR, 1 H & 13 C NMR, ESI-MS, EPR} and magnetic susceptibility measurements. The structure activity relationship (SAR) studies of benzothiazole Cu(II) & Zn(II) complexes having molecular formulas [C30 H22 CuN5 O7 S2 ], [C30 H20 Cl2 CuN5 O7 S2 ], [C30 H20 CuF2 N5 O7 S2 ], [C30 H22 N4 O4 S2 Zn], [C30 H20 Cl2 N4 O4 S2 Zn] and [C30 H20 F2 N5 O7 S2 Zn], with CT‒DNA were performed by employing absorption, emission titrations and hydrodynamic measurements...
April 20, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29676090/-effect-of-ingestion-on-antithrombin-activity-in-different-tissues-of-whitmania-pigra
#20
Man-Jun Wu, Qiao-Sheng Guo, Hong-Zhuan Shi, Fei Liu, Guo-Wei Shi, Bin Wu
To study the effects of ingestion on antithrombin activities in different tissues of Whitmania pigra, the salivary glands, ingluvies, intestine and muscle of adult leeches were weighted on the 1st d, 3rd d, 5th d, 7th d and 11th d after feeding, respectively, and meanwhile antithrombin activity was measured by antithrombin titration method. The results showed that the antithrombin activity of salivary glands in different stages was significantly higher than that in other tissues ( P <0.05). The activity of antithrombin in muscle tissue increased initially and then decreased with the prolongation of the time after feeding, and the peak was observed on the 5th day after feeding ( P <0...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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