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microflora cd8

Xia Luo, Zengfeng Pan, Shuang Luo, Qi Liu, Shaowei Huang, Guanghua Yang, Feifei Nong, Yajun Fu, Xiangliang Deng, Lian Zhou
Both probiotics and pathogens in the human gut express pathogen-associated molecular patterns (PAMPs) and die with the release of endotoxin and bacterial DNA, which can stimulate our immune system and cause immune reaction. However, it's interesting and fascinating to address why the normal intestinal flora will not generate immunological rejection like the pathogen does. By investigating the changes in cells and molecules relevant to immune tolerance in mice with ceftriaxone-induced dysbacteriosis, our study discovered that both the Evenness indexes and Shannon Wiener index of intestinal flora showed a decrease in dysbacteriosis mice...
May 14, 2018: International Immunopharmacology
Shahram Agah, Ali Mohammad Alizadeh, Maryam Mosavi, Peyman Ranji, Hossein Khavari-Daneshvar, Farnaz Ghasemian, Sahar Bahmani, Abbas Tavassoli
Based on the ability of the probiotics in the gut microflora modification, they can have the beneficial effects on diseases in the short and/or the long term. In previous study, we revealed that unlike Bifidobacterium bifidum, the amount of Lactobacillus acidophilus remained almost unchanged in mice gut microflora in the long term, indicating more stability of L. acidophilus than B. bifidum which can be used to prevent some incurable diseases such as cancer. Thirty-eight male BALB/c mice were divided into four groups, control, azoxymethane (AOM), L...
April 22, 2018: Probiotics and Antimicrobial Proteins
Ashley D Markazi, Victor Perez, Mamduh Sifri, Revathi Shanmugasundaram, Ramesh K Selvaraj
Three separate experiments were conducted to study the effects of whole yeast cell product supplementation in pullets and layer hens. Body weight gain, fecal and intestinal coccidial oocyst counts, cecal microflora species, cytokine mRNA amounts, and CD4+ and CD8+ T-cell populations in the cecal tonsils were analyzed following an experimental coccidial infection. In Experiment I, day-old Leghorn layer chicks were fed 3 experimental diets with 0, 0.1, or 0.2% whole yeast cell product (CitriStim®, ADM, Decatur, IL)...
July 1, 2017: Poultry Science
Sisi Lin, Yongyu Li, Li Shen, Ruiqin Zhang, Lizhi Yang, Min Li, Kun Li, Jakub Fichna
BACKGROUND: Ulcerative colitis (UC) is strongly associated with inflammation and intestinal barrier disorder. The nonselective cannabinoid receptor agonist HU210 has been shown to ameliorate inflamed colon in colitis, but its effects on intestinal barrier function and extraintestinal inflammation are unclear. AIMS: To investigate the effects and the underlying mechanism of HU210 action on the UC in relation to a role of TLR4 and MAP kinase signaling. METHODS: Wild-type (WT) and TLR4 knockout (Tlr4-/- ) mice were exposed to 4% dextran sulfate sodium (DSS) for 7 days...
February 2017: Digestive Diseases and Sciences
Shi-Fa Zhang, Zong-Sheng Tang, Ling Tong, Xiang-Xiang Tao, Qi-Feng Suo, Xue-Mei Xu
BACKGROUND & AIMS: Probiotics is recognized to promote growth performance and immune function via balancing the intestinal microflora. Live clostridium butyricum and bifidobacterium combined powder (LCBBCP) has been widely to treat intestinal dysbacteriosis in newborns in China. This study was undertaken to investigate the effects of the combined probiotics on the expression of B and T lymphocyte attenuator (BTLA) on CD4(+) T cells and the differentiation of lymphocyte subsets in late preterm infants...
November 2016: Microbial Pathogenesis
Q-F Gui, H-F Lu, C-X Zhang, Z-R Xu, Y-H Yang
The intestinal microflora affects inflammation and immunity, not only locally at the mucosal level but also systemically, raising the question of whether the microflora affects inflammatory processes that contribute to cancer and its therapy. Prebiotics have also been found to play an antitumor role that is not limited to the gut. We investigated the antitumor roles of the intestinal microbiota using the Lewis lung cancer mouse model. In mice treated with cisplatin combined with ABX (an antibiotic cocktail of vancomycin, ampicillin, and neomycin), which can destroy the host commensal microflora, the tumor size was larger than in mice on a single treatment of cisplatin...
2015: Genetics and Molecular Research: GMR
Edwin Leeansyah, Liyen Loh, Douglas F Nixon, Johan K Sandberg
Innate-like, evolutionarily conserved MR1-restricted mucosa-associated invariant T (MAIT) cells represent a large antimicrobial T-cell subset in humans. Here, we investigate the development of these cells in second trimester human fetal tissues. MAIT cells are rare and immature in the fetal thymus, spleen and mesenteric lymph nodes. In contrast, mature IL-18Rα(+) CD8αα MAIT cells are enriched in the fetal small intestine, liver and lung. Independently of localization, MAIT cells express CD127 and Ki67 in vivo and readily proliferate in response to Escherichia coli in vitro...
2014: Nature Communications
Nisha Goyal, Geeta Shukla
BACKGROUND: Gut homeostasis can be altered by the oral administration of health-promoting microorganisms, namely probiotics that are known to reinforce the host immune response. AIM: The aim of this study was to elucidate the immunomodulatory effect of orally administered probiotic Lactobacillus rhamnosus GG (LGG) in Giardia-infected mice. METHODS: BALB/c mice were fed orally with probiotic LGG either 7 days prior to or simultaneously with the challenge dose of Giardia trophozoites...
May 2013: Digestive Diseases and Sciences
Sydney E Lambert, Jeremy M Kinder, Jenny E Then, Kelly N Parliament, Heather A Bruns
The mucosal immune system is constantly exposed to antigen, whether it be food antigen, commensal bacteria, or harmful antigen. It is essential that the mucosal immune system can distinguish between harmful and non-harmful antigens, and initiate an active immune response to clear the harmful antigens, while initiating a suppressive immune response (tolerance) to non-harmful antigens. Oral tolerance is an immunologic hyporesponsiveness to an orally administered antigen and is important in preventing unnecessary gastrointestinal tract inflammation, which can result in a number of autoimmune and hypersensitivity diseases...
2012: Frontiers in Immunology
Jae-Ouk Kim, Hye-Ran Cha, Eun-Do Kim, Mi-Na Kweon
Interleukin (IL)-17A is a cytokine that plays an important role in infectious, autoimmune, and inflammatory diseases. In this study, we found that TCRγδ(+)CD4(-)CD8(-) T cells, but not TCRαβ(+)CD4(+) T cells, are the primary producers of IL-17A in the genital tract of female mice in the steady-state condition. High mRNA levels of IL-17A and RORγt were determined in TCRγδ(+) T cells isolated from mouse genital tract but lacked detectable expression of IFNγ, T-bet, and FoxP3. IL-17A production by genital TCRγδ(+) T cells was maintained after intravaginal vaccination with cholera toxin or avirulent herpes simplex virus type (HSV)-2 186 syn ΔTK strain...
September 2012: Immunology Letters
Shabnam Shalapour, Katrin Deiser, Anja A Kühl, Rainer Glauben, Susanne M Krug, André Fischer, Ozen Sercan, Stephane Chappaz, Stefan Bereswill, Markus M Heimesaat, Christoph Loddenkemper, Michael Fromm, Daniela Finke, Günter J Hämmerling, Bernd Arnold, Britta Siegmund, Thomas Schüler
Interleukin-7 (IL-7) is a major survival factor for mature T cells. Therefore, the degree of IL-7 availability determines the size of the peripheral T cell pool and regulates T cell homeostasis. Here we provide evidence that IL-7 also regulates the homeostasis of intestinal epithelial cells (IEC), colon function and the composition of the commensal microflora. In the colon of T cell-deficient, lymphopenic mice, IL-7-producing IEC accumulate. IEC hyperplasia can be blocked by IL-7-consuming T cells or the inactivation of the IL-7/IL-7R signaling pathway...
2012: PloS One
Paulo César Giraldo, Joziani Beghini Junqueira de Carvalho, Rose Luce Gomes do Amaral, Ana Katherine da Silveira Gonçalves, José Eleutério, Fernando Guimarães
PROBLEM:   The extent of the vaginal immune response is not fully determined. The purpose of this study was to evaluate the vaginal immune cells from women with vulvovaginitis (VV). METHOD OF STUDY: A total of 142 volunteers diagnosed with bacterial vaginosis (BV), vulvovaginal candidiasis (VC), and BV associated with VC or normal microflora were sampled to evaluate the immune cells by flow cytometry. The immune cells were obtained by vaginal lavage and labeled with fluorochrome-conjugated monoclonal antibodies to identify neutrophil granulocytes, macrophages, CD4(+) and CD8(+) T lymphocytes, B lymphocytes, and NK lymphocytes...
March 2012: American Journal of Reproductive Immunology: AJRI
Zhi-Gang Ren, Hui Liu, Jian-Wen Jiang, Li Jiang, Hui Chen, Hai-Yang Xie, Lin Zhou, Shu-Sen Zheng
BACKGROUND: Most patients waiting for liver transplantation have end-stage liver diseases with malnutrition, which is prone to induce intestinal barrier dysfunction after liver transplantation. We aimed to study the effect of probiotics on intestinal barrier function in malnourished rats following liver transplantation with long-term antibiotics. METHODS: Twelve Lewis rats were selected as donors. Twelve BN rats, which served as recipients, were subjected to malnutrition by semi-starvation for 4-5 weeks...
October 2011: Hepatobiliary & Pancreatic Diseases International: HBPD INT
Patrick N Fries, Yurij I Popowych, Le Luo Guan, Philip J Griebel
Mucosal dendritic cells (DCs) play a key role in discriminating between dietary antigens, commensal microflora and pathogens but little is known regarding age-related changes in mucosal DC populations. We analyzed lymphoid and myeloid populations within the epithelium and lamina propria (LP) of the ileum and jejunum of weaned calves (6 months old) and compared their frequency and distribution with newborn calves (3-5 weeks old). CD4, CD8 and γδ TcR T cells and CD11c(Hi)MHC Class II(+) myeloid cell frequency were significantly different when comparing ileum and jejunum of weaned calves...
2011: Cellular Immunology
Martin Levkut, Viera Revajová, Andrea Lauková, Zuzana Ševčíková, Viera Spišáková, Zita Faixová, Mária Levkutová, Viola Strompfová, Juraj Pistl, Mikuláš Levkut
The protective effect of Enterococcus faecium EF55 in chickens challenged with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was assessed. The antibacterial effect on the bacterial microflora in the small intestine in relation to white blood cell count, phenotyping of peripheral blood and intestinal lymphocytes, functional activity of lymphocytes and phagocytes and mucin quantitation were investigated. Day-old chicks (85) were randomly divided into four groups. The probiotic group (EF) and Salmonella+probiotic group (EFSE) received E...
August 2012: Research in Veterinary Science
K G Shapovalov, T E Belokrinitskaia, Zh S Burdinskaia, A V Maliarchikov
AIM: To study features of immune status and causative agents of severe pneumonia in patients with influenza A/H1N1. MATERIALS AND METHODS: Fifty-seven isolates from 43 patients with pneumonia, which complicated pandemic influenza A/ H1N1 and treated in Chita city clinical hospital No.1 as well as 32 immunograms were retrospectively studied. Comparison of immunologic and epidemiologic status of patients with influenza A/ H1N1 complicated by pneumonia was performed...
January 2011: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
Yuko Kato-Mori, Takenori Orihashi, Yuta Kanai, Michiko Sato, Kenji Sera, Katsuro Hagiwara
Lactobacillus gasseri OLL 2716 promotes the elimination of Helicobacter pylori and is utilized in yogurts that are specifically labeled as health foods. On the other hand, milk whey fermented by Propionibacterium freudenreichii ET-3, which increases the numbers of Bifidobacterium, is effective for intestinal disorders. We previously demonstrated that oral administration of L. gasseri and P. freudenreichii fermentation metabolites (LP-FM) improved calf intestinal microflora and reduced the incidence of diarrhea...
December 2010: Journal of Medicinal Food
Roxana Valdés-Ramos, Beatriz E Martínez-Carrillo, Irma I Aranda-González, Ana Laura Guadarrama, Rosa Virgen Pardo-Morales, Patricia Tlatempa, Rosa A Jarillo-Luna
Diet and exercise are primary strategies recommended for the control of the obesity epidemic. Considerable attention is being paid to the effect of both on the immune system. However, little research has been done on the effect of diet, nutrients or exercise on the mucosal immune system. The gastrointestinal tract (gut) is not only responsible for the entry of nutrients into the organism, but also for triggering the primary immune response to orally ingested antigens. The gut-associated lymphoid tissue contains a large amount of immune cells, disseminated all along the intestine in Peyer's patches and lamina propria...
November 2010: Proceedings of the Nutrition Society
Jianfeng Gong, Jingyue Xu, Weiming Zhu, Xiang Gao, Ning Li, Jieshou Li
Accumulating evidence suggests a role for Toll-like receptor (TLR) signaling at the intestinal epithelial cells (IECs) level for intestinal protection against exogenous injury or pathogenic infection. We hypothesized that MyD88 dependent TLR signaling at intestinal epithelium is critical for mucosal immune homeostasis. In the current study, a transgenic mouse model was generated in which a dominant-negative mutant of MyD88 (dnMyD88) was driven by an intestinal epithelial-specific murine villin promoter. Aged transgenic mice spontaneously developed chronic small intestinal inflammation, as revealed by increased CD4+ and CD8+ lymphocytes, neutrophil and macrophage infiltration, increased production of cytokines as TNF-alpha, IFN-gamma, IL-1beta, and IL-17, crypt abscesses, lymphedema, and Goblet cell depletion...
August 2010: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Y B Shen, X S Piao, S W Kim, L Wang, P Liu, I Yoon, Y G Zhen
A total of 216 weaning pigs were used in 2 experiments to determine the effects of dietary supplementation of yeast culture (YC) at different dose levels on the growth performance, nutrient digestibility, intestinal morphology, intestinal microflora, and immune response in weanling pigs and to determine whether YC can be a candidate to replace antibiotic growth promoters (AGP). In Exp. 1, 192 pigs (7.5 +/- 0.2 kg of BW) weaned at 28 d of age were randomly allotted to 6 treatments: 1) control (without AGP or YC); 2) AGP (chlortetracycline, 80 mg/kg); 3) 2...
August 2009: Journal of Animal Science
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