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RNA sequencing endometrium

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https://www.readbyqxmd.com/read/28125717/epigenetic-alterations-affecting-transcription-factors-and-signaling-pathways-in-stromal-cells-of-endometriosis
#1
Iveta Yotova, Emily Hsu, Catherine Do, Aulona Gaba, Matthias Sczabolcs, Sabine Dekan, Lukas Kenner, Rene Wenzl, Benjamin Tycko
Endometriosis is characterized by growth of endometrial-like tissue outside the uterine cavity. Since its pathogenesis may involve epigenetic changes, we used Illumina 450K Methylation Beadchips to profile CpG methylation in endometriosis stromal cells compared to stromal cells from normal endometrium. We validated and extended the Beadchip data using bisulfite sequencing (bis-seq), and analyzed differential methylation (DM) at the CpG-level and by an element-level classification for groups of CpGs in chromatin domains...
2017: PloS One
https://www.readbyqxmd.com/read/27940919/massive-dysregulation-of-genes-involved-in-cell-signaling-and-placental-development-in-cloned-cattle-conceptus-and-maternal-endometrium
#2
Fernando H Biase, Chanaka Rabel, Michel Guillomot, Isabelle Hue, Kalista Andropolis, Colleen A Olmstead, Rosane Oliveira, Richard Wallace, Daniel Le Bourhis, Christophe Richard, Evelyne Campion, Aurélie Chaulot-Talmon, Corinne Giraud-Delville, Géraldine Taghouti, Hélène Jammes, Jean-Paul Renard, Olivier Sandra, Harris A Lewin
A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients before or during the implantation window. We investigated this problem by using RNA sequencing (RNA-seq) to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at day (d) 18 (preimplantation) and d 34 (postimplantation) of gestation. In addition, endometrium was profiled to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before or during the implantation window...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27913250/identification-of-non-coding-and-coding-rnas-in-porcine-endometrium
#3
Yueying Wang, Tao Hu, Lihang Wu, Xiaoran Liu, Songyi Xue, Minggang Lei
One of the most critical periods of embryonic loss in pig is day 12 of pregnancy, when implantation begins. Here, we analyzed the gene expression on day 12 of pregnancy and non-pregnancy in the porcine endometrium using RNA sequencing (RNA-seq). 237 mRNAs, 34 lncRNAs and 1 miRNA were significantly differentially expressed between the two groups. Further functional analyses were conducted to identify these differentially expressed transcripts. The results demonstrated that they participate in various biological processes, such as cell adhesion, binding, nucleic and metabolic processes...
January 2017: Genomics
https://www.readbyqxmd.com/read/27887719/leucine-rich-repeat-containing-g-protein-coupled%C3%A2-receptor%C3%A2-5-positive%C3%A2-cells%C3%A2-in-the-endometrial-stem-cell-niche
#4
Irene Cervelló, Claudia Gil-Sanchis, Xavier Santamaría, Amparo Faus, Julia Vallvé-Juanico, Patricia Díaz-Gimeno, Oriana Genolet, Antonio Pellicer, Carlos Simón
OBJECTIVE: To study, isolate and characterize leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5 (LGR5)-positive cells from human endometrium to determine their functional relevance. DESIGN: Prospective experimental animal study. SETTING: University research laboratories. ANIMAL(S): Nonobese diabetic mice (NOD-SCID) (strain code 394; NOD.CB17-Prkdc(scid)/NcrCrl). INTERVENTION(S): Human LGR5(+) cells were labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and injected under the kidney capsule in immunocompromised mice...
February 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27760749/circulating-mirnas-as-potential-alternative-cell-signaling-associated-with-maternal-recognition-of-pregnancy-in-the-mare
#5
Kristin M Klohonatz, Ashely D Cameron, Joanna R Hergenreder, Juliano C da Silveira, Aeriel D Belk, D N R Veeramachaneni, Gerrit J Bouma, Jason E Bruemmer
During early pregnancy, the conceptus and mare communicate to establish pregnancy. Cell-secreted vesicles (e.g., exosomes) have been reported in serum. Exosomes contain bioactive materials, such as miRNA, that can mediate cell responses. We hypothesized that a) exosomes are present in mare circulation and quantity varies with pregnancy status, b) exosomes contain miRNAs unique to pregnancy status, and c) miRNAs target pathways in endometrium based upon pregnancy status of the mare. First, serum samples were obtained from mares in a crossover design, with each mare providing samples from a pregnant and nonmated control cycle (n = 3/sample day) on Days 12, 14, 16, and 18 postovulation...
December 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27417907/identification-of-beef-heifers-with-superior-uterine-capacity-for-pregnancy
#6
Thomas W Geary, Gregory W Burns, Joao G N Moraes, James I Moss, Anna C Denicol, Kyle B Dobbs, M Sofia Ortega, Peter J Hansen, Michael E Wehrman, Holly Neibergs, Eleanore O'Neil, Susanta Behura, Thomas E Spencer
Infertility and subfertility represent major problems in domestic animals and humans, and the majority of embryonic loss occurs during the first month of gestation that involves pregnancy recognition and conceptus implantation. The critical genes and physiological pathways in the endometrium that mediate pregnancy establishment and success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select animals with intrinsic differences in pregnancy loss...
August 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27412245/interleukin-1-receptor-antagonist-mediates-toll-like-receptor-3-induced-inhibition-of-trophoblast-adhesion-to-endometrial-cells-in-vitro
#7
M Montazeri, J A Sanchez-Lopez, I Caballero, N Maslehat Lay, S Elliott, A Fazeli
STUDY QUESTION: Is interleukin-1 receptor antagonist (IL-1RA) involved in the toll-like receptor 3 (TLR 3)-induced inhibition of trophoblast cells' adhesion to endometrial cells in vitro? SUMMARY ANSWER: IL-1RA mediates the TLR 3-induced inhibition of trophoblast cells' adhesion to endometrial cells in vitro. WHAT IS KNOWN ALREADY: It is well documented that endometrial TLR 3 activation leads to impairment of trophoblast binding to endometrial cells in vitro...
September 2016: Human Reproduction
https://www.readbyqxmd.com/read/27281703/virus-mediated-metalloproteinase-1-induction-revealed-by-transcriptome-profiling-of-bovine-herpesvirus-4-infected-bovine-endometrial-stromal-cells
#8
Giulia Tebaldi, Sarah Jacca, Barbara Montanini, Emanuele Capra, Alfonso Rosamilia, Arianna Sala, Alessandra Stella, Bianca Castiglioni, Simone Ottonello, Gaetano Donofrio
Viral infections can cause genital tract disorders (including abortion) in cows, and bovine herpesvirus 4 (BoHV-4) is often present in endometritis-affected animals. A major problem with cattle uterine viral infections in general, and BoHV-4 in particular, is our limited understanding of the pathogenic role(s) that these infections play in the endometrium. A similar lack of knowledge holds for the molecular mechanisms utilized, and the host cell pathways affected, by BoHV-4. To begin to fill these gaps, we set up optimized conditions for BoHV-4 infection of a pure population of bovine endometrial stromal cells (BESCs) to be used as source material for RNA sequencing-based transcriptome profiling...
July 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27207143/genome-wide-analysis-of-the-transcriptional-response-to-porcine-reproductive-and-respiratory-syndrome-virus-infection-at-the-maternal-fetal-interface-and-in-the-fetus
#9
Jamie M Wilkinson, Hua Bao, Andrea Ladinig, Linjun Hong, Paul Stothard, Joan K Lunney, Graham S Plastow, John C S Harding
BACKGROUND: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant pigs can result in congenital infection and ultimately fetal death. Little is known about immune responses to infection at the maternal-fetal interface and in the fetus itself, or the molecular events behind virus transmission and disease progression in the fetus. To investigate these processes, RNA-sequencing of two sites, uterine endothelium with adherent placental tissue and fetal thymus, was performed 21 days post-challenge on four groups of fetuses selected from a large PRRSV challenge experiment of pregnant gilts: control (CON), uninfected (UNINF), infected (INF), and meconium-stained (MEC) (n = 12/group)...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27039075/an-investigation-of-the-microbiota-in-uterine-flush-samples-and-endometrial-biopsies-from-dairy-cows-during-the-first-7%C3%A2-weeks-postpartum
#10
Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Øystein Angen, Jørgen Steen Agerholm, Eva Láadal Rasmussen, Tim Kåre Jensen, Kirstine Klitgaard
Metritis and endometritis commonly occur in dairy cows after calving. Although numerous studies have been performed to identify the causative pathogens, a complete overview has not been done. Metagenomic studies have analyzed the bacterial populations of uterine flush samples from postpartum (pp) dairy cows, but the microbiota in the uterine luminal fluid may differ from the microbiota of the endometrium itself, and important putative pathogens may have been overlooked. In the present study, we compared the microbiota of the uterine lumen and the endometrium of healthy, metritic, and endometritic cows...
July 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27035670/the-promyelocytic-leukemia-zinc-finger-transcription-factor-is-critical-for-human-endometrial-stromal-cell-decidualization
#11
Ramakrishna Kommagani, Maria M Szwarc, Yasmin M Vasquez, Mary C Peavey, Erik C Mazur, William E Gibbons, Rainer B Lanz, Francesco J DeMayo, John P Lydon
Progesterone, via the progesterone receptor (PGR), is essential for endometrial stromal cell decidualization, a cellular transformation event in which stromal fibroblasts differentiate into decidual cells. Uterine decidualization supports embryo implantation and placentation as well as subsequent events, which together ensure a successful pregnancy. Accordingly, impaired decidualization results not only in implantation failure or early fetal miscarriage, but also may lead to potential adverse outcomes in all three pregnancy trimesters...
April 2016: PLoS Genetics
https://www.readbyqxmd.com/read/26981354/endometrial-transcriptional-profiling-of-a-bovine-fertility-model-by-next-generation-sequencing
#12
F S Mesquita, R S Ramos, G Pugliesi, S C S Andrade, V Van Hoeck, A Langbeen, M L Oliveira, A M Gonella-Diaza, G Gasparin, H Fukumasu, L H Pulz, C M Membrive, L L Coutinho, M Binelli
Studying the multitude of molecular networks and pathways that are potentially involved in a complex trait such as fertility requires an equally complex and broad strategy. Here, we used Next-Generation Sequencing for the characterization of the transcriptional signature of the bovine endometrial tissue. Periovulatory endocrine environments were manipulated to generate two distinctly different fertility phenotypes. Cycling, non-lactating, multiparous Nelore cows were manipulated to ovulate larger (> 13 mm; LF group; high fertility phenotype) or smaller (< 12 mm; SF group) follicles...
March 2016: Genomics Data
https://www.readbyqxmd.com/read/26874359/single-cell-transcriptome-analysis-of-endometrial-tissue
#13
COMPARATIVE STUDY
K Krjutškov, S Katayama, M Saare, M Vera-Rodriguez, D Lubenets, K Samuel, T Laisk-Podar, H Teder, E Einarsdottir, A Salumets, J Kere
STUDY QUESTION: How can we study the full transcriptome of endometrial stromal and epithelial cells at the single-cell level? SUMMARY ANSWER: By compiling and developing novel analytical tools for biopsy, tissue cryopreservation and disaggregation, single-cell sorting, library preparation, RNA sequencing (RNA-seq) and statistical data analysis. WHAT IS KNOWN ALREADY: Although single-cell transcriptome analyses from various biopsied tissues have been published recently, corresponding protocols for human endometrium have not been described...
April 2016: Human Reproduction
https://www.readbyqxmd.com/read/26823997/characterisation-of-the-human-uterine-microbiome-in-non-pregnant-women-through-deep-sequencing-of-the-v1-2-region-of-the-16s-rrna-gene
#14
Hans Verstraelen, Ramiro Vilchez-Vargas, Fabian Desimpel, Ruy Jauregui, Nele Vankeirsbilck, Steven Weyers, Rita Verhelst, Petra De Sutter, Dietmar H Pieper, Tom Van De Wiele
Background. It is widely assumed that the uterine cavity in non-pregnant women is physiologically sterile, also as a premise to the long-held view that human infants develop in a sterile uterine environment, though likely reflecting under-appraisal of the extent of the human bacterial metacommunity. In an exploratory study, we aimed to investigate the putative presence of a uterine microbiome in a selected series of non-pregnant women through deep sequencing of the V1-2 hypervariable region of the 16S ribosomal RNA (rRNA) gene...
2016: PeerJ
https://www.readbyqxmd.com/read/26822553/analyses-of-long-non-coding-rna-and-mrna-profiling-using-rna-sequencing-during-the-pre-implantation-phases-in-pig-endometrium
#15
Yueying Wang, Songyi Xue, Xiaoran Liu, Huan Liu, Tao Hu, Xiaotian Qiu, Jinlong Zhang, Minggang Lei
Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison...
January 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/26488136/the-human-endometrium-specific-proteome-defined-by-transcriptomics-and-antibody-based-profiling
#16
Agata Zieba, Evelina Sjöstedt, Matts Olovsson, Linn Fagerberg, Björn M Hallström, Linda Oskarsson, Karolina Edlund, Anna Tolf, Mathias Uhlen, Fredrik Ponten
The human uterus includes the complex endometrial mucosa, the endometrium that undergoes dynamic, hormone-dependent alterations throughout the life of fertile females. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to analyze gene expression patterns in the normal endometrium. Human endometrial tissues from five women were used for deep sequencing (RNA-Seq). The mRNA and protein expression data from the endometrium were compared to 31 (RNA) and 44 (protein) other normal tissue types, to identify genes with elevated expression in the endometrium and to localize the expression of corresponding proteins at a cellular resolution...
November 2015: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/26435523/endometrial-gene-expression-profile-of-pregnant-sows-with-extreme-phenotypes-for-reproductive-efficiency
#17
S Córdoba, I Balcells, A Castelló, C Ovilo, J L Noguera, O Timoneda, A Sánchez
Prolificacy can directly impact porcine profitability, but large genetic variation and low heritability have been found regarding litter size among porcine breeds. To identify key differences in gene expression associated to swine reproductive efficiency, we performed a transcriptome analysis of sows' endometrium from an Iberian x Meishan F2 population at day 30-32 of gestation, classified according to their estimated breeding value (EBV) as high (H, EBV > 0) and low (L, EBV < 0) prolificacy phenotypes...
October 5, 2015: Scientific Reports
https://www.readbyqxmd.com/read/26303215/identification-of-mirnas-in-bovine-endometrium-through-rnaseq-and-prediction-of-regulated-pathways
#18
S E Palma-Vera, S Sharbati, R Einspanier
Detection of miRNAs in reproductive tissues is a key step to understand their role in fertility. We hypothesize that miRNAs must be involved in pathways controlling endometrial physiology and defense against pathogens. In this study, we aimed to characterize miRNAs present in bovine endometrium and to predict regulated pathways. Cytobrush endometrial samples from four cows were collected at oestrous cycle days 1-5, 6-12, 13-18 and 19-21. RNA was extracted and sequenced using Ion Torrent (®) technology. After mapping of the reads to miRNA stem loops, rRNAs and tRNAs, data were normalized and analysed using DESeq2...
October 2015: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/25947061/-conceptualizing-the-endometrium-identification-of-conceptus-derived-proteins-during-early-pregnancy-in-cattle
#19
Niamh Forde, Fuller W Bazer, Thomas E Spencer, Pat Lonergan
The aim of this study was to identify conceptus-derived proteins, in addition to IFNT, that may facilitate pregnancy recognition in cattle. Analysis of the protein content of the uterine luminal fluid (ULF) from cyclic heifers on Day 16 by nano liquid chromatography tandem mass spectrometry identified 334 proteins. Comparison of these data with 299 proteins identified in the ULF of pregnant heifers on Day 16 identified 85 proteins only present in the ULF of pregnant heifers. Analysis of Day 16 conceptus-conditioned culture medium revealed the presence of 1005 proteins of which 30 proteins were unique to ULF from Day 16 pregnant heifers...
June 2015: Biology of Reproduction
https://www.readbyqxmd.com/read/25940503/novel-equine-conceptus-endometrial-interactions-on-day-16-of-pregnancy-based-on-rna-sequencing
#20
C Klein
Maintenance of pregnancy is dependent on the exchange of signals between the conceptus and the endometrium. The objective of this study was to use next-generation sequencing to determine transcriptome blueprints of the conceptus and endometrium 16 days after ovulation in the horse. There were 7760 and 10 182 genes expressed in the conceptus and endometrium, respectively, of which 7029 were present in both. Genes related to developmental processes were enriched among conceptus-specific transcripts, whereas many endometrium-specific genes had known roles in cell communication, cell adhesion and response to stimuli...
May 5, 2015: Reproduction, Fertility, and Development
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