Read by QxMD icon Read

RNA sequencing endometrium

Kristin M Klohonatz, Ashley D Cameron, Joanna R Hergenreder, Juliano C da Silveira, Aeriel D Belk, D N R Veeramachaneni, Gerrit J Bouma, Jason E Bruemmer
During early pregnancy the conceptus and mare communicate to establish pregnancy. Cell-secreted vesicles (e.g. exosomes) have been reported in serum. Exosomes contain bioactive materials, such as miRNA, that can mediate cell responses. We hypothesized that (i) exosomes are present in mare circulation and quantity varies with pregnancy status, (ii) exosomes contain miRNAs unique to pregnancy status, and (iii) miRNAs target pathways in endometrium based upon pregnancy status of the mare. First, serum samples were obtained from mares in a crossover design, with each mare providing samples from a pregnant and non-mated control cycle (n = 3/sample day) on Days 12, 14, 16, and 18 post-ovulation...
October 19, 2016: Biology of Reproduction
Thomas W Geary, Gregory W Burns, Joao G N Moraes, James I Moss, Anna C Denicol, Kyle B Dobbs, M Sofia Ortega, Peter J Hansen, Michael E Wehrman, Holly Neibergs, Eleanore O'Neil, Susanta Behura, Thomas E Spencer
Infertility and subfertility represent major problems in domestic animals and humans, and the majority of embryonic loss occurs during the first month of gestation that involves pregnancy recognition and conceptus implantation. The critical genes and physiological pathways in the endometrium that mediate pregnancy establishment and success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select animals with intrinsic differences in pregnancy loss...
August 2016: Biology of Reproduction
M Montazeri, J A Sanchez-Lopez, I Caballero, N Maslehat Lay, S Elliott, A Fazeli
STUDY QUESTION: Is interleukin-1 receptor antagonist (IL-1RA) involved in the toll-like receptor 3 (TLR 3)-induced inhibition of trophoblast cells' adhesion to endometrial cells in vitro? SUMMARY ANSWER: IL-1RA mediates the TLR 3-induced inhibition of trophoblast cells' adhesion to endometrial cells in vitro. WHAT IS KNOWN ALREADY: It is well documented that endometrial TLR 3 activation leads to impairment of trophoblast binding to endometrial cells in vitro...
September 2016: Human Reproduction
Giulia Tebaldi, Sarah Jacca, Barbara Montanini, Emanuele Capra, Alfonso Rosamilia, Arianna Sala, Alessandra Stella, Bianca Castiglioni, Simone Ottonello, Gaetano Donofrio
Viral infections can cause genital tract disorders (including abortion) in cows, and bovine herpesvirus 4 (BoHV-4) is often present in endometritis-affected animals. A major problem with cattle uterine viral infections in general, and BoHV-4 in particular, is our limited understanding of the pathogenic role(s) that these infections play in the endometrium. A similar lack of knowledge holds for the molecular mechanisms utilized, and the host cell pathways affected, by BoHV-4. To begin to fill these gaps, we set up optimized conditions for BoHV-4 infection of a pure population of bovine endometrial stromal cells (BESCs) to be used as source material for RNA sequencing-based transcriptome profiling...
July 2016: Biology of Reproduction
Jamie M Wilkinson, Hua Bao, Andrea Ladinig, Linjun Hong, Paul Stothard, Joan K Lunney, Graham S Plastow, John C S Harding
BACKGROUND: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant pigs can result in congenital infection and ultimately fetal death. Little is known about immune responses to infection at the maternal-fetal interface and in the fetus itself, or the molecular events behind virus transmission and disease progression in the fetus. To investigate these processes, RNA-sequencing of two sites, uterine endothelium with adherent placental tissue and fetal thymus, was performed 21 days post-challenge on four groups of fetuses selected from a large PRRSV challenge experiment of pregnant gilts: control (CON), uninfected (UNINF), infected (INF), and meconium-stained (MEC) (n = 12/group)...
2016: BMC Genomics
Lif Rødtness Vesterby Knudsen, Cecilia Christensen Karstrup, Hanne Gervi Pedersen, Øystein Angen, Jørgen Steen Agerholm, Eva Láadal Rasmussen, Tim Kåre Jensen, Kirstine Klitgaard
Metritis and endometritis commonly occur in dairy cows after calving. Although numerous studies have been performed to identify the causative pathogens, a complete overview has not been done. Metagenomic studies have analyzed the bacterial populations of uterine flush samples from postpartum (pp) dairy cows, but the microbiota in the uterine luminal fluid may differ from the microbiota of the endometrium itself, and important putative pathogens may have been overlooked. In the present study, we compared the microbiota of the uterine lumen and the endometrium of healthy, metritic, and endometritic cows...
July 15, 2016: Theriogenology
Ramakrishna Kommagani, Maria M Szwarc, Yasmin M Vasquez, Mary C Peavey, Erik C Mazur, William E Gibbons, Rainer B Lanz, Francesco J DeMayo, John P Lydon
Progesterone, via the progesterone receptor (PGR), is essential for endometrial stromal cell decidualization, a cellular transformation event in which stromal fibroblasts differentiate into decidual cells. Uterine decidualization supports embryo implantation and placentation as well as subsequent events, which together ensure a successful pregnancy. Accordingly, impaired decidualization results not only in implantation failure or early fetal miscarriage, but also may lead to potential adverse outcomes in all three pregnancy trimesters...
April 2016: PLoS Genetics
F S Mesquita, R S Ramos, G Pugliesi, S C S Andrade, V Van Hoeck, A Langbeen, M L Oliveira, A M Gonella-Diaza, G Gasparin, H Fukumasu, L H Pulz, C M Membrive, L L Coutinho, M Binelli
Studying the multitude of molecular networks and pathways that are potentially involved in a complex trait such as fertility requires an equally complex and broad strategy. Here, we used Next-Generation Sequencing for the characterization of the transcriptional signature of the bovine endometrial tissue. Periovulatory endocrine environments were manipulated to generate two distinctly different fertility phenotypes. Cycling, non-lactating, multiparous Nelore cows were manipulated to ovulate larger (> 13 mm; LF group; high fertility phenotype) or smaller (< 12 mm; SF group) follicles...
March 2016: Genomics Data
K Krjutškov, S Katayama, M Saare, M Vera-Rodriguez, D Lubenets, K Samuel, T Laisk-Podar, H Teder, E Einarsdottir, A Salumets, J Kere
STUDY QUESTION: How can we study the full transcriptome of endometrial stromal and epithelial cells at the single-cell level? SUMMARY ANSWER: By compiling and developing novel analytical tools for biopsy, tissue cryopreservation and disaggregation, single-cell sorting, library preparation, RNA sequencing (RNA-seq) and statistical data analysis. WHAT IS KNOWN ALREADY: Although single-cell transcriptome analyses from various biopsied tissues have been published recently, corresponding protocols for human endometrium have not been described...
April 2016: Human Reproduction
Hans Verstraelen, Ramiro Vilchez-Vargas, Fabian Desimpel, Ruy Jauregui, Nele Vankeirsbilck, Steven Weyers, Rita Verhelst, Petra De Sutter, Dietmar H Pieper, Tom Van De Wiele
Background. It is widely assumed that the uterine cavity in non-pregnant women is physiologically sterile, also as a premise to the long-held view that human infants develop in a sterile uterine environment, though likely reflecting under-appraisal of the extent of the human bacterial metacommunity. In an exploratory study, we aimed to investigate the putative presence of a uterine microbiome in a selected series of non-pregnant women through deep sequencing of the V1-2 hypervariable region of the 16S ribosomal RNA (rRNA) gene...
2016: PeerJ
Yueying Wang, Songyi Xue, Xiaoran Liu, Huan Liu, Tao Hu, Xiaotian Qiu, Jinlong Zhang, Minggang Lei
Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and conceptus development. We investigated the expression profiles of endometrial tissue on Days 9, 12 and 15 of pregnancy and on Day 12 of non-pregnancy in Yorkshire, and performed a comprehensive analysis of long non-coding RNAs (lncRNAs) in endometrial tissue samples by using RNA sequencing. As a result, 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison...
2016: Scientific Reports
Agata Zieba, Evelina Sjöstedt, Matts Olovsson, Linn Fagerberg, Björn M Hallström, Linda Oskarsson, Karolina Edlund, Anna Tolf, Mathias Uhlen, Fredrik Ponten
The human uterus includes the complex endometrial mucosa, the endometrium that undergoes dynamic, hormone-dependent alterations throughout the life of fertile females. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to analyze gene expression patterns in the normal endometrium. Human endometrial tissues from five women were used for deep sequencing (RNA-Seq). The mRNA and protein expression data from the endometrium were compared to 31 (RNA) and 44 (protein) other normal tissue types, to identify genes with elevated expression in the endometrium and to localize the expression of corresponding proteins at a cellular resolution...
November 2015: Omics: a Journal of Integrative Biology
S Córdoba, I Balcells, A Castelló, C Ovilo, J L Noguera, O Timoneda, A Sánchez
Prolificacy can directly impact porcine profitability, but large genetic variation and low heritability have been found regarding litter size among porcine breeds. To identify key differences in gene expression associated to swine reproductive efficiency, we performed a transcriptome analysis of sows' endometrium from an Iberian x Meishan F2 population at day 30-32 of gestation, classified according to their estimated breeding value (EBV) as high (H, EBV > 0) and low (L, EBV < 0) prolificacy phenotypes...
2015: Scientific Reports
S E Palma-Vera, S Sharbati, R Einspanier
Detection of miRNAs in reproductive tissues is a key step to understand their role in fertility. We hypothesize that miRNAs must be involved in pathways controlling endometrial physiology and defense against pathogens. In this study, we aimed to characterize miRNAs present in bovine endometrium and to predict regulated pathways. Cytobrush endometrial samples from four cows were collected at oestrous cycle days 1-5, 6-12, 13-18 and 19-21. RNA was extracted and sequenced using Ion Torrent (®) technology. After mapping of the reads to miRNA stem loops, rRNAs and tRNAs, data were normalized and analysed using DESeq2...
October 2015: Reproduction in Domestic Animals, Zuchthygiene
Niamh Forde, Fuller W Bazer, Thomas E Spencer, Pat Lonergan
The aim of this study was to identify conceptus-derived proteins, in addition to IFNT, that may facilitate pregnancy recognition in cattle. Analysis of the protein content of the uterine luminal fluid (ULF) from cyclic heifers on Day 16 by nano liquid chromatography tandem mass spectrometry identified 334 proteins. Comparison of these data with 299 proteins identified in the ULF of pregnant heifers on Day 16 identified 85 proteins only present in the ULF of pregnant heifers. Analysis of Day 16 conceptus-conditioned culture medium revealed the presence of 1005 proteins of which 30 proteins were unique to ULF from Day 16 pregnant heifers...
June 2015: Biology of Reproduction
C Klein
Maintenance of pregnancy is dependent on the exchange of signals between the conceptus and the endometrium. The objective of this study was to use next-generation sequencing to determine transcriptome blueprints of the conceptus and endometrium 16 days after ovulation in the horse. There were 7760 and 10 182 genes expressed in the conceptus and endometrium, respectively, of which 7029 were present in both. Genes related to developmental processes were enriched among conceptus-specific transcripts, whereas many endometrium-specific genes had known roles in cell communication, cell adhesion and response to stimuli...
May 5, 2015: Reproduction, Fertility, and Development
Ke Chen, Xuemei Chen, Junlin He, Yubin Ding, Yanqing Geng, Shangjing Liu, Xueqing Liu, Yingxiong Wang
Embryo implantation is a dynamic physiological process involving morphological and molecular changes in the endometrium during the pre-receptivity, receptivity, and implantation phases. A comprehensive analysis of messenger RNA (mRNA) and microRNA (miRNA) profiles during implantation will likely provide new clues to elucidate the underlying mechanisms governing embryo implantation. We characterized the mRNA and miRNA transcriptomes using next generation sequencing (NGS) of the endometrium 1 day postcoitum (dpc) and 4dpc and the implantation site (IMS) and inter-implantation (IIM) site of the endometrium on 5dpc...
November 2015: Reproductive Sciences
Rahaba Mojakgomo, Zukile Mbita, Zodwa Dlamini
Ceramide synthases (CerSs) also known as Longevity Assurance (LASS) genes belong to a family of six related genes. CerS gene products have been shown to produce ceramide, hence their name CerSs. Ceramide is a bio-effector molecule, belonging to the family of sphingolipids (SLs), which are important components of cell membranes, and has been implicated in cancer and apoptosis. Cancer still remains the second leading cause of death, both globally and in South Africa. The proper regulation of the balance between cell growth and cell death is essential for cellular homeostasis...
June 2015: Experimental and Molecular Pathology
Maria Ravo, Angela Cordella, Antonio Rinaldi, Giuseppina Bruno, Elena Alexandrova, Pasquale Saggese, Giovanni Nassa, Giorgio Giurato, Roberta Tarallo, Giovanna Marchese, Francesca Rizzo, Claudia Stellato, Rossella Biancardi, Jacopo Troisi, Attilio Di Spiezio Sardo, Fulvio Zullo, Alessandro Weisz, Maurizio Guida
Small non-coding RNAs (sncRNAs) represent a heterogeneous group of <200nt-long transcripts comprising microRNAs, PIWI-interacting RNAs (piRNAs) and small-nucleolar-RNAs (snoRNAs) involved in physiological and pathological processes such as carcinogenesis and tumor progression. Aberrant sncRNA expression in cancer has been associated with specific clinical phenotypes, grading, staging, metastases development and resistance to therapy.Aim of the present work is to study the role of sncRNAs in endometrial carcinogenesis...
March 10, 2015: Oncotarget
Yurika Tachibana, Toshihiro Sakurai, Hanako Bai, Kunio Shiota, Yasuo Nambo, Kentaro Nagaoka, Kazuhiko Imakawa
Extensive studies have been conducted to characterize the unique phenomena of equine pregnancy. Most studies have focused on embryo transmigration when the embryo is covered with a mucin-like glycoprotein capsule and on the characterization of the chorionic girdle and chorionic gonadotropin (CG) secretion. However, the events preceding and following capsule disappearance have not been well studied. In this study, the mRNA expression in conceptus membranes at days 19, 21, and 25 (day 0 = day of ovulation) was analyzed by RNA-seq (SOLiD3), and transcript levels on these three days and day 13 were confirmed by real-time PCR...
2014: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"