Xiao-Chuan Li, Wei Wang, Cheng Jiang, Yong-Long Chen, Jiong-Hui Chen, Zhen-Wu Zhang, Shao-Jian Luo, Rong-Chun Chen, Ping-Fan Mo, Ming-Liang Zhong, Jiang-You Shi, Chun-Ming Huang, Qin Chen, Yao-Hong Wu
OBJECTIVE: This study aimed to explore the specific function of M2 macrophages in intervertebral disc degeneration (IDD). METHODS: Intervertebral disc (IVD) samples from normal (n=4) and IDD (n=6) patients were collected, and the expression of M2-polarized macrophage marker, CD206, was investigated using immunohistochemical staining. Nucleus pulposus cells (NPCs) in a TNF-α environment were obtained, and a mouse caudal IVD puncture model was established. Mice with Rheb deletions, specifically in the myeloid lineage, were generated and subjected to surgery-induced IDD...
October 4, 2023: Osteoarthritis and Cartilage