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https://www.readbyqxmd.com/read/29456845/low-carbonyl-reductase-1-expression-is-associated-with-poor-prognosis-in-patients-with-oral-squamous-cell-carcinoma
#1
Ryota Yamanouchi, Koji Harada, Tarannum Ferdous, Yoshiya Ueyama
Carbonyl reductase 1 (CBR1) is an enzyme that catalyzes the reduction of numerous compounds by using NADPH-dependent oxidoreductase activity. Decreased expression of CBR1 is associated with disease progression and an unfavorable outcome in several types of malignancies. The purpose of the current study was to determine whether CBR1 expression could be a useful prognostic factor in patients with oral squamous cell carcinoma (OSCC). Therefore, its mechanisms of action were investigated in order to understand how CBR1 affects cancer cell behavior in vitro ...
March 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29454806/oxidative-imbalance-in-mice-intoxicated-by-microcystin-lr-can-be-minimized
#2
Natália V Casquilho, Maria Diana Moreira-Gomes, Clarissa B Magalhães, Renata T Okuro, Victor Hugo Ortenzi, Emanuel K Feitosa-Lima, Lidia M Lima, Eliezer J Barreiro, Raquel M Soares, Sandra M F O Azevedo, Samuel S Valença, Rodrigo S Fortunato, Alysson Roncally Carvalho, Walter A Zin
Microcystins-LR (MC-LR) is a cyanotoxin produced by cyanobacteria. We evaluated the antioxidant potential of LASSBio-596 (LB-596, inhibitor of phosphodiesterases 4 and 5), per os, and biochemical markers involved in lung and liver injury induced by exposure to sublethal dose of MC-LR. Fifty male Swiss mice received an intraperitoneal injection of 60 μL of saline (CTRL group, n = 20) or a sublethal dose of MC-LR (40 μg/kg, TOX group, n = 20). After 6 h the animals received either saline (TOX and CTRL groups) or LB-596 (50 mg/kg, TOX + LASS group, n = 10) by gavage...
February 15, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29454792/clinical-and-genomic-correlates-of-neutrophil-reactive-oxygen-species-production-in-pediatric-patients-with-crohn-s-disease
#3
Lee A Denson, Ingrid Jurickova, Rebekah Karns, Kelly A Shaw, David J Cutler, David Okou, Anne Dodd, Kathryn Quinn, Kajari Mondal, Bruce J Aronow, Yael Haberman, Aaron Linn, Adam Price, Ramona Bezold, Kathleen Lake, Kimberly Jackson, Thomas D Walters, Anne Griffiths, Robert N Baldassano, Joshua D Noe, Jeffrey S Hyams, Wallace V Crandall, Barbara S Kirschner, Melvin B Heyman, Scott Snapper, Stephen L Guthery, Marla C Dubinsky, Neal S Leleiko, Anthony R Otley, Ramnik J Xavier, Christine Stevens, Mark J Daly, Michael E Zwick, Subra Kugathasan
BACKGROUND & AIMS: Individuals with monogenic disorders of phagocyte function develop chronic colitis that resembles Crohn's Disease (CD). We tested for associations between mutations in genes encoding NADPH oxidases, neutrophil function, and phenotypes of CD in pediatric patients. METHODS: We performed whole-exome sequence analysis to identify mutations in genes encoding NADPH oxidases (such as CYBA, CYBB, NCF1, NCF2, NCF4, RAC1, and RAC2) using DNA from 543 pediatric patients with inflammatory bowel diseases...
February 15, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29454348/rational-engineering-of-streptomyces-albus-j1074-for-the-overexpression-of-secondary-metabolite-gene-clusters
#4
Dimitris Kallifidas, Guangde Jiang, Yousong Ding, Hendrik Luesch
BACKGROUND: Genome sequencing revealed that Streptomyces sp. can dedicate up to ~ 10% of their genomes for the biosynthesis of bioactive secondary metabolites. However, the majority of these biosynthetic gene clusters are only weakly expressed or not at all. Indeed, the biosynthesis of natural products is highly regulated through integrating multiple nutritional and environmental signals perceived by pleiotropic and pathway-specific transcriptional regulators. Although pathway-specific refactoring has been a proved, productive approach for the activation of individual gene clusters, the construction of a global super host strain by targeting pleiotropic-specific genes for the expression of multiple diverse gene clusters is an attractive approach...
February 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29452861/corrigendum-to-thapsigargin-triggers-cardiac-contractile-dysfunction-via-nadph-oxidase-mediated-mitochondrial-dysfunction-role-of-akt-dephosphorylation-free-radic-biol-med-51-12-2011-2172-2184
#5
https://www.readbyqxmd.com/read/29452237/a-redox-sensitive-signaling-pathway-mediates-pro-angiogenic-effect-of-chlordecone-via-estrogen-receptor-activation
#6
Eid Alabed Alibrahim, Ramaroson Andriansitohaina, Kévin Hardonnière, Raffaella Soleti, Sébastien Faure, Gilles Simard
AIM: Chlordecone is able to induce pro-angiogenic effect through an estrogen receptor (ERα) pathway involving NO release and VEGF. The present study aimed to determine the molecular mechanisms by which chlordecone promotes angiogenesis in human endothelial cells. RESULTS: High but not low concentration of chlordecone increased mitochondrial respiratory capacity and mitochondrial DNA content in endothelial cells. The ROS scavenger MnTMPyP was able to prevent the increase of both VEGF expression and capillary length induced by chlordecone...
February 13, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29451786/the-catalytic-reaction-of-nitroxides-with-peroxyl-radicals-and-its-relevance-to-their-cytoprotective-properties
#7
Markus Griesser, Ron Shah, Antonius T Van Kessel, Omkar Zilka, Evan A Haidasz, Derek A Pratt
Sterically-hindered nitroxides such as 2,2,6,6-tetramethylpiperidin-N-oxyl (TEMPO) have long been ascribed antioxidant activity that is thought to underlie their chemopreventive and anti-aging properties. However, the most commonly invoked reactions in this context - combination with an alkyl radical to give a redox inactive alkoxyamine or the catalysis of superoxide dismutation - are unlikely to be relevant under (most) physiological conditions. Herein we characterize the kinetics and mechanisms of the reactions of TEMPO, as well as an N-arylnitroxide and a N,N-diarylnitroxide, with alkylperoxyl radicals, the propagating species in lipid peroxidation...
February 16, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29449592/first-evidence-for-a-multienzyme-complex-of-lipid-biosynthesis-pathway-enzymes-in-cunninghamella-bainieri
#8
Shuwahida Shuib, Izyanti Ibrahim, Mukram Mohamed Mackeen, Colin Ratledge, Aidil Abdul Hamid
Malic enzyme (ME) plays a vital role in determining the extent of lipid accumulation in oleaginous fungi being the major provider of NADPH for the activity of fatty acid synthase (FAS). We report here the first direct evidence of the existence of a lipogenic multienzyme complex (the lipid metabolon) involving ME, FAS, ATP: citrate lyase (ACL), acetyl-CoA carboxylase (ACC), pyruvate carboxylase (PC) and malate dehydrogenase (MDH) in Cunninghamella bainieri 2A1. Cell-free extracts prepared from cells taken in both growth and lipid accumulation phases were prepared by protoplasting and subjected to Blue Native (BN)-PAGE coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29449441/role-of-nadph-oxidase-2-and-oxidative-stress-in-children-exposed-to-passive-smoking
#9
Lorenzo Loffredo, Anna Maria Zicari, Francesca Occasi, Ludovica Perri, Roberto Carnevale, Francesco Angelico, Maria Del Ben, Francesco Martino, Cristina Nocella, Giovanna De Castro, Vittoria Cammisotto, Simona Battaglia, Marzia Duse, Francesco Violi
This study explored oxidative stress, nicotinamide adenine dinucleotide phosphate oxidase-2 (Nox2) activity and endothelial function in children exposed or not to passive smoking. Compared with controls (n=57), Nox2 activity and isoprostanes were higher in children exposed to passive smoking (n=57); conversely, nitric oxide (NO) bioavailability and flow-mediated dilation were lower in children exposed to passive smoking. A bivariate analysis showed that Nox2 activity correlated with flow-mediated dilation, NO bioavailability and isoprostanes...
February 15, 2018: Thorax
https://www.readbyqxmd.com/read/29448137/differential-metabolism-of-l-phenylalanine-in-the-formation-of-aromatic-volatiles-in-melon-cucumis-melo-l-fruit
#10
Itay Gonda, Rachel Davidovich-Rikanati, Einat Bar, Shery Lev, Pliaa Jhirad, Yuval Meshulam, Guy Wissotsky, Vitaly Portnoy, Joseph Burger, Arthur A Schaffer, Yaakov Tadmor, James J Giovannoni, Zhangjun Fei, Aaron Fait, Nurit Katzir, Efraim Lewinsohn
Studies on the active pathways and the genes involved in the biosynthesis of L-phenylalanine-derived volatiles in fleshy fruits are sparse. Melon fruit rinds converted stable-isotope labeled L-phe into more than 20 volatiles. Phenylpropanes, phenylpropenes and benzenoids are apparently produced via the well-known phenylpropanoid pathway involving phenylalanine ammonia lyase (PAL) and being (E)-cinnamic acid a key intermediate. Phenethyl derivatives seemed to be derived from L-phe via a separate biosynthetic route not involving (E)-cinnamic acid and PAL...
February 12, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29448107/decrease-in-the-cytosolic-nadp-dependent-isocitrate-dehydrogenase-activity-through-porcine-sperm-capacitation
#11
Yuki Katoh, Michiko Tamba, Manabu Matsuda, Kazuhiro Kikuchi, Naomichi Okamura
In order to understand the molecular mechanisms involved in the sperm capacitation, we have identified the proteins tyrosine-phosphorylated during the capacitation especially in conjunction with the regulation of the levels of reactive oxygen species (ROS) in sperm. In the present study, the effects of the tyrosine phosphorylation of cytosolic NADP + -dependent isocitrate dehydrogenase (IDPc) on its catalytic activity and on the levels of ROS in sperm have been studied. The tyrosine phosphorylated IDPc showed a significantly lowered enzymatic activity...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29447059/therapeutic-potential-of-mucuna-pruriens-linn-on-ageing-induced-damage-in-dorsal-nerve-of-the-penis-and-its-implication-on-erectile-function-an-experimental-study-using-albino-rats
#12
Prakash Seppan, Ibrahim Muhammed, Karthik Ganesh Mohanraj, Ganesh Lakshmanan, Dinesh Premavathy, Sakthi Jothi Muthu, Khayinmi Wungmarong Shimray, Sathya Bharathy Sathyanathan
OBJECTIVE: To study the effect of ethanolic seed extract of Mucuna pruriens on damaged dorsal nerve of the penis (DNP) in aged rat in relation to penile erection. METHODS: The rats were divided into four groups Young (3 months), Aged (24 - 28 months), Aged + M. pruriens, and Young + M. pruriens (200 mg/kg b.w/60 days) and were subjected to the hypophysial - gonadal axis, nerve conduction velocity (NCV), and penile reflex. DNP sections were stained with nitric oxide synthase (nNOS), nicotinamide adenine dinucleotide phosphate (NaDPH) diaphorase, androgen receptor (AR), and osmium tetroxide...
February 15, 2018: Aging Male: the Official Journal of the International Society for the Study of the Aging Male
https://www.readbyqxmd.com/read/29445778/nadph-oxidase-4-driven-cardiac-macrophage-polarization-protects-against-myocardial-infarction-induced-remodeling
#13
Heloise Mongue-Din, Ashish S Patel, Yee H Looi, David J Grieve, Narayana Anilkumar, Alexander Sirker, Xuebin Dong, Alison C Brewer, Min Zhang, Alberto Smith, Ajay M Shah
The reactive oxygen species-generating enzyme NADPH oxidase 4 (Nox4) is up-regulated in the heart after myocardial infarction (MI). Mice with cardiomyocyte-targeted Nox4 overexpression (TG) displayed increased macrophages in the heart at baseline, with skewing toward an M2 phenotype compared with wild-type controls (WT). After MI, TG mice had a higher proportion of M2 macrophages along with higher survival, decreased cardiac remodeling, and better contractile function than wild-type mice. The post-MI increase in cardiac matrix metalloproteinase-2 activity was substantially blunted in TG mice...
December 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/29445382/innovative-approaches-to-evaluate-sugar-beet-responses-to-changes-in-sulfate-availability
#14
Piergiorgio Stevanato, Chiara Broccanello, Vita M C Moliterni, Giuseppe Mandolino, Valeria Barone, Luigi Lucini, Giovanni Bertoldo, Marco Bertaggia, Massimo Cagnin, Diego Pizzeghello, Andrea Baglieri, Andrea Squartini, Giuseppe Concheri, Serenella Nardi
In this study, a system based on omics profiling was set-up for sugar beet ( Beta vulgaris L. subsp. vulgaris ) evaluation after changes in sulfate availability. Seedlings were grown on sulfate-deprived Hoagland solution. Six days after germination, 100 μM MgSO 4 was added to the solution. Root samples were collected 36 h after treatments. WinRHIZO root-scanning approach was used for the automated image analysis of plant root morphology. Inductively Coupled Plasma Spectrometry (ICP-OES) and quadrupole-time-of-flight mass spectrometry (Q-TOF) were used for ionomic and metabolic analysis, respectively...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29445082/pyruvate-kinase-m2-regulates-photoreceptor-structure-function-and-viability
#15
Ammaji Rajala, Yuhong Wang, Richard S Brush, Kristine Tsantilas, Connor S R Jankowski, Ken J Lindsay, Jonathan D Linton, James B Hurley, Robert E Anderson, Raju V S Rajala
Pyruvate kinase M2 (PKM2) is a glycolytic enzyme that is expressed in cancer cells. Its role in tumor metabolism is not definitively established, but investigators have suggested that regulation of PKM2 activity can cause accumulation of glycolytic intermediates and increase flux through the pentose phosphate pathway. Recent evidence suggests that PKM2 also may have non-metabolic functions, including as a transcriptional co-activator in gene regulation. We reported previously that PKM2 is abundant in photoreceptor cells in mouse retinas...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29444417/a-study-on-the-regenerative-effect-of-platelets-rich-plasma-on-experimentally-induced-hepatic-damage-in-albino-rats
#16
Heba Mamdoh Shoeib, Walaa Arafa Keshk, Abdallah Mahmoud Foda, Saad El-Deen Abd Elfatah Abo El Noeman
Hepatic fibrosis is a worldwide health problem with significant morbidity and mortality. Currently, there is no effective therapy for hepatic fibrosis. So that the present study was aimed to evaluate the possible regenerative effect of Platelet-rich plasma (PRP) against thioacetamide (TAA) induced hepatic damage. Eighty albino rats were included; 40 were used for PRP preparation and 40 were randomly divided into four groups. Group I (control group); group II (PRP control); group III (TAA- intoxicated in a dose of 200 mg ∕ kg body weight/twice weekly for 7 weeks, intra-peritoneal and group IV (TAA-intoxicated+ PRP treated)...
February 14, 2018: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29444316/glucose-triggers-stomatal-closure-mediated-by-gin1-gin2-pyr-rcar-ros-no-cdpk6-and-slac1-in-arabidposis
#17
Yan Li, Shanshan Xu, Zhiwei Wang, Lingchao He, Kang Xu, Genxuan Wang
Sugars play important roles in regulating plant growth, development and stomatal movement. Here, we found that glucose triggered stomatal closure, showing a dose-and time-dependent manner in Arabidopsis. Pharmacological data showed that glucose-induced stomatal closure was greatly inhibited by catalase (CAT, a reactive oxygen species (ROS) scavenger), diphenyleneiodonium chloride (DPI, an NADPH oxidase inhibitor), lanthanum chloride (LaCl3, a Ca2+ channel blocker), ethylene glycol tetraacetic acid (EGTA, a Ca2+ chelator), two nitrate reductase (NR) inhibitors, tungstate and sodium azide (NaN3), while not affected by salicylhydroxamic acid (SHAM, a peroxidase inhibitor)...
February 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29443418/potential-roles-of-ycf54-and-ferredoxin-nadph-reductase-for-magnesium-protoporphyrin-monomethylester-cyclase
#18
Josephine Herbst, Annabel Girke, Mohammad Reza Hajirezaei, Guy Hanke, Bernhard Grimm
Chlorophyll is synthesized from activated glutamate in the tetrapyrrole biosynthesis pathway through at least 20 different enzymatic reactions. Among these, the MgProto monomethylester (MgProtoME) cyclase catalyzes the formation of a fifth isocyclic ring to tetrapyrroles to form protochlorophyllide. The enzyme consists of two proteins. The CHL27 protein is proposed to be the catalytic component, while LCAA/YCF54 likely acts as a scaffolding factor. In comparison to other reactions of chlorophyll biosynthesis, this enzymatic step lacks clear elucidation and it is hardly understood, how electrons are delivered for the NADPH-dependent cyclization reaction...
February 14, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29441583/photoenzymes-and-related-topics-an-update
#19
Lars Olof Björn
Photoenzymes are enzymes that catalyze photochemical reactions. For a long time it was believed that only two types of photoenzymes exist: Light-dependent NADPH:protochlorophyllide oxidoreductase and photolyase. However, other photoenzymes have now been discovered, most recently fatty acid photodecarboxylase. This article is protected by copyright. All rights reserved.
February 13, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29441570/inhibition-of-nox4-dependent-ros-signaling-attenuates-prostate-fibroblast-activation-and-abrogates-stromal-mediated-pro-tumorigenic-interactions
#20
Natalie Sampson, Elena Brunner, Anja Weber, Martin Puhr, Georg Schäfer, Cedric Szyndralewiez, Helmut Klocker
Carcinoma-associated fibroblasts (CAFs) play a key onco-supportive role during prostate cancer (PCa) development and progression. We previously reported that the reactive oxygen species (ROS)-producing enzyme NADPH oxidase 4 (Nox4) is essential for TGFβ1-mediated activation of primary prostate human fibroblasts to a CAF-like phenotype. This study aimed to further investigate the functional relevance of prostatic Nox4 and determine whether pharmacological inhibition of stromal Nox4 abrogates paracrine-mediated PCa-relevant processes...
February 14, 2018: International Journal of Cancer. Journal International du Cancer
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