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https://www.readbyqxmd.com/read/29913181/ionic-basis-of-cardiac-electrophysiology-in-zebrafish-compared-to-human-hearts
#1
REVIEW
Ursula Ravens
Zebrafish heart has evolved as an intensely studied model for cardiac development, physiology and pathophysiology. A particular advantage is its morphological and functional similarity to mammalian, especially human heart. Zebrafish are simple to breed, uncomplicated to handle and easy to modify genetically. Because of the transparency of the early embryo, drug effects on heart rate and rhythm can be observed directly. Here we will compare the ionic basis of the electrophysiological properties of the zebrafish to those of other mammalian hearts and discuss the suitability of zebrafish as a model organism for human electrophysiology...
June 15, 2018: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29913180/identification-of-the-actc1c-cardiac-actin-gene-in-zebrafish
#2
Matiyo Ojehomon, Sarah L Alderman, Love Sandhu, Sierra Sutcliffe, Terry Van Raay, Todd E Gillis, John F Dawson
Zebrafish is rapidly becoming a key model organism for studying a variety of biological processes from molecules to organisms. Interactions involving actin, a contractile protein and part of the cytoskeleton, are regulated by actin binding proteins in the majority of physiological processes in eukaryotic cells. To understand the contribution of actin proteins to the physiological processes of zebrafish, it is important to identify the diverse isoforms of actin encoded by its genome; however, significant sequence identity complicates isoform assignments...
June 15, 2018: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29912192/ablation-of-a-neuronal-population-using-a-two-photon-laser-and-its-assessment-using-calcium-imaging-and-behavioral-recording-in-zebrafish-larvae
#3
Akira Muto, Koichi Kawakami
To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ablation of neurons is an effective method for this purpose when neurons are selectively labeled with fluorescent probes. In the present study, protocols for laser ablating a subpopulation of neurons using a two-photon microscope and testing of its functional and behavioral consequences are described. In this study, prey capture behavior in zebrafish larvae is used as a study model...
June 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29911105/genetics-of-cardiovascular-disease-fishing-for-causality
#4
REVIEW
Christoph Paone, Federica Diofano, Deung-Dae Park, Wolfgang Rottbauer, Steffen Just
Cardiovascular disease (CVD) is still the leading cause of death in all western world countries and genetic predisposition in combination with traditional risk factors frequently mediates their manifestation. Genome-wide association (GWA) studies revealed numerous potentially disease modifying genetic loci often including several SNPs and associated genes. However, pure genetic association does not prove direct or indirect relevance of the modifier region on pathogenesis, nor does it define within the associated region the exact genetic driver of the disease...
2018: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/29910763/zebrafish-as-an-animal-model-for-drug-discovery-in-parkinson-s-disease-and-other-movement-disorders-a-systematic-review
#5
Rita L Vaz, Tiago F Outeiro, Joaquim J Ferreira
Movement disorders can be primarily divided into hypokinetic and hyperkinetic. Most of the hypokinetic syndromes are associated with the neurodegenerative disorder Parkinson's disease (PD). By contrast, hyperkinetic syndromes encompass a broader array of diseases, including dystonia, essential tremor, or Huntington's disease. The discovery of effective therapies for these disorders has been challenging and has also involved the development and characterization of accurate animal models for the screening of new drugs...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29909064/development-of-the-astyanax-mexicanus-circadian-clock-and-non-visual-light-responses
#6
Inga A Frøland Steindal, Andrew Beale, Yoshiyuki Yamamoto, David Whitmore
Most animals and plants live on the planet exposed to periods of rhythmic light and dark. As such, they have evolved endogenous circadian clocks to regulate their physiology rhythmically, and non-visual light detection mechanisms to set the clock to the environmental light-dark cycle. In the case of fish, circadian pacemakers are not only present in the majority of tissues and cells, but these tissues are themselves directly light-sensitive, expressing a wide range of opsin photopigments. This broad non-visual light sensitivity exists to set the clock, but also impacts a wide range of fundamental cell biological processes, such as DNA repair regulation...
June 14, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29906474/acteoside-protects-against-6-ohda-induced-dopaminergic-neuron-damage-via-nrf2-are-signaling-pathway
#7
Maiquan Li, Fei Zhou, Tao Xu, Huaxin Song, Baiyi Lu
Acteoside has been reported to have antioxidant and neuroprotective effect, which is a promising therapeutic way in prevention and treatment of Parkinson's disease. The present study was aimed to understand the neuroprotective effect of acteoside and to elucidate its underlying mechanism. 6-hydroxydopamine (6-OHDA)-induced neural damage in zebrafish model was used to study the protective effect of acteoside on Parkinson disease (PD). Locomotion behavioral test showed that acteoside could prevent 6-OHDA-stimulated movement disorders...
June 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29905858/optimized-knock-in-of-point-mutations-in-zebrafish-using-crispr-cas9
#8
Sergey V Prykhozhij, Charlotte Fuller, Shelby L Steele, Chansey J Veinotte, Babak Razaghi, Johane M Robitaille, Christopher R McMaster, Adam Shlien, David Malkin, Jason N Berman
We have optimized point mutation knock-ins into zebrafish genomic sites using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 reagents and single-stranded oligodeoxynucleotides. The efficiency of knock-ins was assessed by a novel application of allele-specific polymerase chain reaction and confirmed by high-throughput sequencing. Anti-sense asymmetric oligo design was found to be the most successful optimization strategy. However, cut site proximity to the mutation and phosphorothioate oligo modifications also greatly improved knock-in efficiency...
June 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29905491/estimation-of-dynamic-systems-for-gene-regulatory-networks-from-dependent-time-course-data
#9
Yoonji Kim, Jaejik Kim
Dynamic system consisting of ordinary differential equations (ODEs) is a well-known tool for describing dynamic nature of gene regulatory networks (GRNs), and the dynamic features of GRNs are usually captured through time-course gene expression data. Owing to high-throughput technologies, time-course gene expression data have complex structures such as heteroscedasticity, correlations between genes, and time dependence. Since gene experiments typically yield highly noisy data with small sample size, for a more accurate prediction of the dynamics, the complex structures should be taken into account in ODE models...
June 15, 2018: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/29904527/first-year-course-based-undergraduate-research-experience-cure-using-the-crispr-cas9-genome-engineering-technology-in-zebrafish
#10
Jay M Bhatt, Anil Kumar Challa
Genetic analysis in model systems can provide a rich context for conceptual understanding of gene structure, regulation, and function. With an intent to create a rich learning experience in molecular genetics, we developed a semester-long course-based undergraduate research experience (CURE) using the CRISPR-Cas9 gene editing system to disrupt specific genes in the zebrafish. The course was offered to freshman students; nine students worked in four groups (two to three members per group) to design, synthesize, and test the nuclease activity of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/sgRNAs for targeted disruption of specific genes in the zebrafish...
2018: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/29904386/costimulatory-function-of-cd58-cd2-interaction-in-adaptive-humoral-immunity-in-a-zebrafish-model
#11
Tong Shao, Wei Shi, Jia-Yu Zheng, Xiao-Xiao Xu, Ai-Fu Lin, Li-Xin Xiang, Jian-Zhong Shao
CD58 and CD2 have long been known as a pair of reciprocal adhesion molecules involved in the immune modulations of CD8+ T and NK-mediated cellular immunity in humans and several other mammals. However, the functional roles of CD58 and CD2 in CD4+ T-mediated adaptive humoral immunity remain poorly defined. Moreover, the current functional observations of CD58 and CD2 were mainly acquired from in vitro assays, and in vivo investigation is greatly limited due to the absence of a Cd58 homology in murine models...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29903879/adipocyte-derived-lipids-mediate-melanoma-progression-via-fatp-proteins
#12
Maomao Zhang, Julie S Di Martino, Robert L Bowman, Nathaniel R Campbell, Sanjeethan C Baksh, Theresa Simon-Vermot, Isabella S Kim, Pearce Haldeman, Chandrani Mondal, Vladimir Yong-Gonzalez, Mohsen Abu-Akeel, Taha Merghoub, Drew R Jones, Xiphias Ge Zhu, Arshi Arora, Charlotte E Ariyan, Kivanc Birsoy, Jedd D Wolchok, Katherine S Panageas, Travis J Hollmann, Jose Javier Bravo-Cordero, Richard M White
Advanced, metastatic melanomas frequently grow in subcutaneous tissues and portend a poor prognosis. Though subcutaneous tissues are largely composed of adipocytes, the mechanisms by which adipocytes influence melanoma are poorly understood. Using in vitro and in vivo models, we find that adipocytes increase proliferation and invasion of adjacent melanoma cells. Additionally, adipocytes directly transfer lipids to melanoma cells, which alters tumor cell metabolism. Adipocyte-derived lipids are transferred to melanoma cells through the FATP/SLC27A family of lipid transporters expressed on the tumor cell surface...
June 14, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29902500/the-role-of-neuro-epithelial-like-and-radial-glial-stem-and-progenitor-cells-in-development-plasticity-and-repair
#13
REVIEW
Benjamin W Lindsey, Zachary J Hall, Aurélie Heuzé, Jean-Stéphane Joly, Vincent Tropepe, Jan Kaslin
Neural stem and progenitor cells (NSPCs) are the primary source of new neurons in the brain and serve critical roles in tissue homeostasis and plasticity throughout life. Within the vertebrate brain, NSPCs are located within distinct neurogenic niches differing in their location, cellular composition, and proliferative behaviour. Heterogeneity in the NSPC population is hypothesized to reflect varying capacities for neurogenesis, plasticity and repair between different neurogenic zones. Since the discovery of adult neurogenesis, studies have predominantly focused on the behaviour and biological significance of adult NSPCs (aNSPCs) in rodents...
June 11, 2018: Progress in Neurobiology
https://www.readbyqxmd.com/read/29901635/rapamycin-treatment-for-amyotrophic-lateral-sclerosis-protocol-for-a-phase-ii-randomized-double-blind-placebo-controlled-multicenter-clinical-trial-rap-als-trial
#14
Jessica Mandrioli, Roberto D'Amico, Elisabetta Zucchi, Annalisa Gessani, Nicola Fini, Antonio Fasano, Claudia Caponnetto, Adriano Chiò, Eleonora Dalla Bella, Christian Lunetta, Letizia Mazzini, Kalliopi Marinou, Gianni Sorarù, Sara de Biasi, Domenico Lo Tartaro, Marcello Pinti, Andrea Cossarizza
INTRODUCTION: Misfolded aggregated proteins and neuroinflammation significantly contribute to amyotrophic lateral sclerosis (ALS) pathogenesis, hence representing therapeutic targets to modify disease expression. Rapamycin inhibits mechanistic target of Rapamycin (mTOR) pathway and enhances autophagy with demonstrated beneficial effects in neurodegeneration in cell line and animal models, improving phenotype in SQSTM1 zebrafish, in Drosophila model of ALS-TDP, and in the TDP43 mouse model, in which it reduced neuronal loss and TDP43 inclusions...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29899843/induction-of-apoptosis-and-suppression-of-tumor-growth-by-nur77-derived-bcl-2-converting-peptide-in-chemoresistant-lung-cancer-cells
#15
Martin C Pearce, John T Gamble, Prasad R Kopparapu, Edmond F O'Donnell, Monica J Mueller, Hyo Sang Jang, Julie A Greenwood, Arnold C Satterthwait, Robert L Tanguay, Xiao-Kun Zhang, Siva Kumar Kolluri
Resistance to chemotherapy is a major cause of treatment failure and poor overall survival in patients with lung cancer. Identification of molecular targets present in resistant cancer cells is essential for addressing therapeutic resistance and prolonging lung cancer patient survival. Members of the B-cell lymphoma 2 (Bcl-2) family of proteins are associated with chemotherapeutic resistance. In this study, we found that pro-survival protein Bcl-2 is upregulated in paclitaxel resistant cells, potentially contributing to chemotherapy resistance...
May 25, 2018: Oncotarget
https://www.readbyqxmd.com/read/29899370/knockdown-of-crispld2-in-zebrafish-identifies-a-novel-network-for-nonsyndromic-cleft-lip-with-or-without-cleft-palate-candidate-genes
#16
Brett T Chiquet, Qiuping Yuan, Eric C Swindell, Lorena Maili, Robert Plant, Jeffrey Dyke, Ryan Boyer, John F Teichgraeber, Matthew R Greives, John B Mulliken, Ariadne Letra, Susan H Blanton, Jacqueline T Hecht
Orofacial development is a multifaceted process involving tightly regulated genetic signaling networks, that when perturbed, lead to orofacial abnormalities including cleft lip and/or cleft palate. We and others have shown an association between the cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2) gene and nonsyndromic cleft lip with or without cleft palate (NSCLP). Further, we demonstrated that knockdown of Crispld2 in zebrafish alters neural crest cell migration patterns resulting in abnormal jaw and palate development...
June 13, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29897878/model-construction-of-niemann-pick-type-c-disease-in-zebrafish
#17
Yusheng Lin, Xiaolian Caia, Guiping Wang, Gang Ouyang, Hong Cao
Niemann-Pick Type C disease (NPC) is a rare human disease, with limited effective treatment options. Most cases of NPC disease are associated with inactivating mutations of the NPC1 gene. However, cellular and molecular mechanisms responsible for the NPC1 pathogenesis remain poorly defined. This is partly due to the lack of a suitable animal model to monitor the disease progression. In this study, we used CRISPR to construct an NPC1-/- zebrafish model, which faithfully reproduced the cardinal pathological features of this disease...
June 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29897731/small-molecule-inhibitors-of-nfkb-reverse-iron-overload-and-hepcidin-deregulation-in-a-zebrafish-model-for-hereditary-hemochromatosis-type-3
#18
Sandeep Basu, Kanika Jalodia, Shashi Ranjan, Jing-Ruey J Yeh, Randall T Peterson, Chetana Sachidanandan
Hereditary hemochromatosis (HH) is one of the most common genetic disorders in Caucasian populations, with no viable therapeutic options except phlebotomy. We describe a zebrafish model of human HH (HH) created by targeted mutagenesis of the gene encoding Transferrin receptor 2 (tfr2). TFR2 mutations in humans lead to HH Type 3, a rare but severe form of the disease. The tfr2 mutant model in zebrafish recapitulates the defining features of HH3; iron overload and suppression of hepcidin, the iron regulatory hormone...
June 13, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29896711/hemodynamic-studies-for-analyzing-the-teratogenic-effects-of-drugs-in-the-zebrafish-embryo
#19
Huseyin C Yalcin
Investigations of teratogenic effects of drugs generally involve testing the drug on animals and zebrafish embryo is a commonly used animal model for that purpose. In these studies, cardiovascular function of the animals needs to be evaluated to reveal the influence of exposure on the development of the cardiovascular system as well as on the growth of the whole animal. Here, relevant microscopy imaging and analysis protocols are described to calculate a variety of hemodynamic parameters for zebrafish embryos exposed to clinical drugs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29896709/immunohistochemical-assessment-as-a-tool-for-investigating-developmental-toxicity-in-zebrafish-danio-rerio
#20
Carla Santos, Maria de Lurdes Pinto
Immunohistochemistry has been proven to be one of the most important ancillary techniques in understanding early development processes as it allows both a focus on specific, individual cell behaviors as well as an expanded view of tissue architecture, critical to the morphogenesis of complex and integrated organ systems. The zebrafish (Danio rerio) is a well-established model in the area of developmental toxicology and immunohistochemistry methods have been extensively applied to embryos and larvae to ascertain abnormal development resulting from toxic exposure...
2018: Methods in Molecular Biology
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