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https://www.readbyqxmd.com/read/29035434/zebrafish-larvae-model-of-dilated-cardiomyopathy-induced-by-terfenadine
#1
Gyojeong Gu, Yirang Na, Hyewon Chung, Seung Hyeok Seok, Hae Young Lee
BACKGROUND AND OBJECTIVES: Dilated cardiomyopathy can be the end-stage of severe cardiac disorders and directly affects the cardiac muscle, inducing cardiomegaly and heart failure (HF). Although a wide variety of animal models are available to study dilated cardiomyopathy, there is no model to assess dilated cardiomyopathy with non-invasive, simple, and large screening methods. METHODS: We developed a dilated cardiomyopathy model in zebrafish larvae using short duration terfenadine, a known cardiotoxic drug that induces ventricular size dilation...
September 20, 2017: Korean Circulation Journal
https://www.readbyqxmd.com/read/29035311/modeling-of-interactions-between-the-zebrafish-hatching-enzyme-zhe1-and-a-series-of-metal-oxide-nanoparticles-nano-qsar-and-causal-analysis-of-inactivation-mechanisms
#2
Natalia Sizochenko, Danuta Leszczynska, Jerzy Leszczynski
The quantitative relationships between the activity of zebrafish ZHE1 enzyme and a series of experimental and physicochemical features of 24 metal oxide nanoparticles were revealed. Vital characteristics of the nanoparticles' structure were reflected using both experimental and theoretical descriptors. The developed quantitative structure-activity relationship model for nanoparticles (nano-QSAR) was capable of predicting the enzyme inactivation based on four descriptors: the hydrodynamic radius, mass density, the Wigner-Seitz radius, and the covalent index...
October 16, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29034215/the-proteome-of-biologically-active-membrane-vesicles-from-piscirickettsia-salmonis-lf-89-type-strain-identifies-plasmid-encoded-putative-toxins
#3
Cristian Oliver, Mauricio A Hernández, Julia I Tandberg, Karla N Valenzuela, Leidy X Lagos, Ronie E Haro, Patricio Sánchez, Pamela A Ruiz, Constanza Sanhueza-Oyarzún, Marcos A Cortés, María T Villar, Antonio Artigues, Hanne C Winther-Larsen, Ruben Avendaño-Herrera, Alejandro J Yáñez
Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29034193/comparative-study-of-herbal-plants-on-the-phenolic-and-flavonoid-content-antioxidant-activities-and-toxicity-on-cells-and-zebrafish-embryo
#4
Hassan Fahmi Ismail, Zanariah Hashim, Wong Tet Soon, Nur Syukriah Ab Rahman, Ain Nabihah Zainudin, Fadzilah Adibah Abdul Majid
Natural antioxidants derived from plants have shown a tremendous inhibitory effect on free radicals in actively metabolizing cells. Overproduction of free radicals increases the risk factor of chronic diseases associated with diabetes, cancer, arthritis and cardiovascular disease. Andrographis paniculata, Cinnamon zeylanicum, Curcuma xanthorrhiza, Eugenia polyantha and Orthosiphon stamineus are ethnomedicinal plants used in the Asian region to treat various illnesses from a common fever to metabolic disease...
October 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29033362/an-effective-feedback-loop-between-cell-cell-contact-duration-and-morphogen-signaling-determines-cell-fate
#5
Vanessa Barone, Moritz Lang, S F Gabriel Krens, Saurabh J Pradhan, Shayan Shamipour, Keisuke Sako, Mateusz Sikora, Călin C Guet, Carl-Philipp Heisenberg
Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification...
October 11, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29031477/acute-developmental-exposure-to-4-hydroxyandrostenedione-has-a-long-term-effect-on-visually-guided-behaviors
#6
Cassie J Gould, Jenna L Wiegand, Victoria P Connaughton
Estrogenic and anti-estrogenic endocrine disrupting compounds (EDCs) are recognized as critical modulators of neural development, including sensory system development. Using the zebrafish model, we tested the effect of transient developmental exposure to a known anti-estrogenic EDC on adult visually-guided behavior. In particular, we exposed zebrafish aged 24-hour post-fertilization (hpf), 72 hpf, or 7-days post-fertilization (dpf) to the aromatase inhibitor 4-hydroxyandrostenedione (4-OH-A) for 24h. After this time, the fish were removed from treatment, placed into control conditions, and reared until adulthood (3-4months) when visually-guided optomotor responses (OMR) were assessed...
October 11, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/29030288/in-vitro-characterization-of-zebrafish-danio-rerio-organic-anion-transporters-oat2a-e
#7
Jelena Dragojević, Ivan Mihaljević, Marta Popović, Roko Zaja, Tvrtko Smital
OATS/Oats are transmembrane proteins that transport a variety of drugs, environmental toxins and endogenous metabolites into the cell. Zebrafish (Danio rerio) has seven OAT orthologs: Oat1, Oat2a-e and Oat3. In this study we specifically address Oat2 (Slc22a7) family. Conserved synteny analysis showed localization of zebrafish oat2 genes on two chromosomes, 11 and 17. All five zebrafish Oats were localized by live cell imaging in membranes of transiently transfected HEK293-T cells, and Oat2a, b, d, and e were confirmed using western blot analysis...
October 10, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29030255/molecular-cloning-and-characterization-of-secretory-carbonic-anhydrase-vi-in-pufferfish-takifugu-rubripes
#8
Kanij Rukshana Sumi, Soo Cheol Kim, Sathishkumar Natarajan, Kap Seong Choi, Myeong Rak Choi, Hoy-Teak Kim, Jong-In Park, Ill-Sup Nou, Kathleen M Gilmour, Kang Hee Kho
Carbonic anhydrase VI (CA VI) has been characterized as a secretory isozyme in mammals. Our present study confirmed the occurrence of CA VI in pufferfish (Takifugu rubripes). In this study, genomic sequence information for the CA VI of pufferfish was used for molecular cloning. We cloned a 1821-bp cDNA sequence, which consisted of a complete coding sequence of 1623bp and a deduced amino acid sequence of 540 amino acids from the open reading frame. A BLAST search indicated that this protein exhibits 53%, 79%, and 67% identity with human, tilapia, and gar CA VI, respectively...
October 10, 2017: Gene
https://www.readbyqxmd.com/read/29029321/rapid-novel-facile-biosynthesized-silver-nanoparticles-from-bacterial-release-induce-biogenicity-and-concentration-dependent-in-vivo-cytotoxicity-with-embryonic-zebrafish-a-mechanistic-insight
#9
Suresh K Verma, Ealisha Jha, Pritam Kumar Panda, Anurag Mishra, Arun Thirumurugan, Biswadeep Das, S K S Parashar, Mrutyunjay Suar
In this study, rapid one step facile synthesis of silver nanoparticles (AgNP) was done using culture supernatant of two Gram positive (B.thuringiensis, S.aureus) and Gram negative (E.coli, S.typhimurium) bacterial strains and were termed as "BTAgNP", "SAAgNP", "ECAgNP" and "STAgNP" respectively. Synthesized AgNPs were well characterized with the help of different standard techniques like FESEM, DLS, UV-Vis spectroscopy and FTIR. Mechanism of AgNPs synthesis was elucidated using in silico approach. In vivo cytotoxicity of synthesized AgNPs was assessed in embryonic Zebrafish model with the help of uptake, oxidative stress and apoptosis induction experimental assays and the mechanism was investigated through in silico approach at the molecular level...
September 28, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29029162/aberrant-global-and-jagged-mediated-notch-signaling-disrupts-segregation-between-wt1-expressing-and-steroidogenic-tissues-in-zebrafish
#10
Chih-Wei Chou, Jamie Lin, Yun-Jin Jiang, Yi-Wen Liu
Though the zebrafish interrenal tissue has been used as a model for steroidogenesis and genesis of the adrenal gland, its specification and morphogenesis is still largely unclear. In this study, we explored how the WT1-expressing cells are segregated from the SF-1-expressing steroidogenic cells in the zebrafish model. The interrenal tissue precursors expressing ff1b, the equivalent of mammalian SF-1, are derived from wt1-expressing pronephric primordia in the zebrafish embryo. Through histochemistry and in situ hybridization, we demonstrated that the size of functionally-differentiated interrenal tissue was significantly increased upon a global inhibition of the Notch signaling pathway, which was accompanied by a disrupted segregation between the wt1- and ff1b-expressing cells...
September 29, 2017: Endocrinology
https://www.readbyqxmd.com/read/29027980/the-plasticizer-bisphenol-a-perturbs-the-hepatic-epigenome-a-systems-level-analysis-of-the-mirnome
#11
Ludivine Renaud, Willian A da Silveira, E Starr Hazard, Jonathan Simpson, Silvia Falcinelli, Dongjun Chung, Oliana Carnevali, Gary Hardiman
Ubiquitous exposure to bisphenol A (BPA), an endocrine disruptor (ED), has raised concerns for both human and ecosystem health. Epigenetic factors, including microRNAs (miRNAs), are key regulators of gene expression during cancer. The effect of BPA exposure on the zebrafish epigenome remains poorly characterized. Zebrafish represents an excellent model to study cancer as the organism develops a disease that resembles human cancer. Using zebrafish as a systems toxicology model, we hypothesized that chronic BPA-exposure impacts the miRNome in adult zebrafish and establishes an epigenome more susceptible to cancer development...
October 13, 2017: Genes
https://www.readbyqxmd.com/read/29027617/zebrafish-as-a-model-to-study-neuroblastoma-development
#12
REVIEW
Mattie J Casey, Rodney A Stewart
Neuroblastoma is a pediatric solid tumor arising from embryonic neural crest progenitor cells that normally generate the peripheral sympathetic nervous system. As such, the location of neuroblastoma tumors is correlated with the distribution of major post-ganglionic clusters throughout the sympathetic chain, with the highest incidence in the adrenal medulla or lumbar sympathetic ganglia (~65%). Neuroblastoma is an enigmatic tumor that can spontaneously regress with minimal treatment or become highly metastatic and develop resistance to aggressive treatments, including radiation and high-dose chemotherapy...
October 13, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29027214/development-of-an-alternative-zebrafish-model-for-drug-induced-intestinal-toxicity
#13
Bokyeong Ryu, C-Yoon Kim, Hanseul Oh, Ukjin Kim, Jin Kim, Cho-Rok Jung, Byoung-Hee Lee, Seungki Lee, Seo-Na Chang, Ji Min Lee, Hyung-Min Chung, Jae-Hak Park
An evaluation of intestinal toxicity is important because the mucosal lining of the gastrointestinal tract is the first barrier for oral xenobiotics. Until now, a rat model has been recommended as the standard intestinal toxicity model and the Caco-2 cell line, originated from a human colon adenocarcinoma, has been used as an alternative to this model, but there are limitations regarding cost-effectiveness and the need for mimicry of the human system. In this study, we investigated whether zebrafish could be a valid alternative to rats and Caco-2 cells as an intestinal toxicity model...
October 13, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29024578/morpho-functional-features-of-the-gonads-of-danio-rerio-the-role-of-brain-derived-neurotrophic-factor
#14
Pietro Cacialli, Livia D'Angelo, Paolo de Girolamo, Luigi Avallone, Carla Lucini, Elisabeth Pellegrini, Luciana Castaldo
Zebrafish, a suitable and widely used teleost fish model in basic biomedical research, displays morphophysiological features of adult gonads that share some commonalities with those of mammalian species. In mammals, gametogenesis is regulated, among several factors, by brain derived neurotrophic factor (BDNF). This neurotrophin has a well-established role in the developing and adult nervous system, as well as gonads development and functions in vertebrates pecies. We hypothesize that BDNF has a role also in the gonadal functions of zebrafish...
October 12, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29024245/hace1-influences-zebrafish-cardiac-development-via-ros-dependent-mechanisms
#15
Babak Razaghi, Shelby L Steele, Sergey V Prykhozhij, Matthew R Stoyek, Jessica A Hill, Matthew D Cooper, Lindsay McDonald, William Lin, Mads Daugaard, Nicolas Crapoulet, Simi Chacko, Stephen Lewis, Ian C Scott, Poul H B Sorensen, Jason N Berman
BACKGROUND: In this study we reveal a previously undescribed role of the HACE1 (HECT domain and Ankyrin repeat Containing E3 ubiquitin-protein ligase 1) tumor suppressor protein in normal vertebrate heart development using the zebrafish (Danio rerio) model. We examined the link between the cardiac phenotypes associated with hace1 loss of function to the expression of the Rho small family GTPase, rac1, which is a known target of HACE1 and promotes ROS production via its interaction with NADPH oxidase holoenzymes...
October 11, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29023224/zebrafish-a-premier-vertebrate-model-for-biomedical-research-in-indian-scenario
#16
Sreeja Sarasamma, Muhammed Muhsin Varikkodan, Sung-Tzu Liang, Yen-Chang Lin, Wen-Pin Wang, Chung-Der Hsiao
The zebrafish (Danio rerio) is a versatile model organism that has been used in biomedical research for several decades to study a wide range of biological phenomena. There are many technical advantages of using zebrafish over other vertebrate models. They are readily available, hardy, easy, and inexpensive to maintain in the laboratory, have a short life cycle, and have excellent fecundity. Due to its optical clarity and reproducible capabilities, it has become one of the predominant models of human genetic diseases...
October 12, 2017: Zebrafish
https://www.readbyqxmd.com/read/29022183/in-search-of-a-comprehensible-set-of-endpoints-for-the-routine-monitoring-of-neurotoxicity-in-vertebrates-sensory-perception-and-nerve-transmission-in-zebrafish-danio-rerio-embryos
#17
Daniel Stengel, Sarah Wahby, Thomas Braunbeck
In order to develop a test battery based on a variety of neurological systems in fish, three sensory systems (vision, olfaction, and lateral line) as well as nerve transmission (acetylcholine esterase) were analyzed in zebrafish (Danio rerio) embryos with respect to their suitability as a model for the screening of neurotoxic trace substances in aquatic ecosystems. As a selection of known or putative neurotoxic compounds, amidotrizoic acid, caffeine, cypermethrin, dichlorvos, 2,4-dinitrotoluene, 2,4-dichlorophenol, 4-nonylphenol, perfluorooctanoic acid, and perfluorooctane sulfonic acid were tested in the fish embryo test (OECD test guideline 236) to determine EC10 values, which were then used as maximum test concentration in subsequent neurotoxicity tests...
October 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29021890/zebrafish-knockout-of-down-syndrome-gene-dyrk1a-shows-social-impairments-relevant-to-autism
#18
Oc-Hee Kim, Hyun-Ju Cho, Enna Han, Ted Inpyo Hong, Krishan Ariyasiri, Jung-Hwa Choi, Kyu-Seok Hwang, Yun-Mi Jeong, Se-Yeol Yang, Kweon Yu, Doo-Sang Park, Hyun-Woo Oh, Erica E Davis, Charles E Schwartz, Jeong-Soo Lee, Hyung-Goo Kim, Cheol-Hee Kim
BACKGROUND: DYRK1A maps to the Down syndrome critical region at 21q22. Mutations in this kinase-encoding gene have been reported to cause microcephaly associated with either intellectual disability or autism in humans. Intellectual disability accompanied by microcephaly was recapitulated in a murine model by overexpressing Dyrk1a which mimicked Down syndrome phenotypes. However, given embryonic lethality in homozygous knockout (KO) mice, no murine model studies could present sufficient evidence to link Dyrk1a dysfunction with autism...
2017: Molecular Autism
https://www.readbyqxmd.com/read/29021554/human-tau-p301l-overexpression-results-in-tau-hyperphosphorylation-without-neurofibrillary-tangles-in-adult-zebrafish-brain
#19
Mehmet I Cosacak, Prabesh Bhattarai, Ledio Bocova, Tim Dzewas, Violeta Mashkaryan, Christos Papadimitriou, Kerstin Brandt, Heike Hollak, Christopher L Antos, Caghan Kizil
Microtubule-associated TAU protein is a pathological hallmark in Alzheimer's disease (AD), where hyperphosphorylation of TAU generates neurofibrillary tangles. To investigate the effects of TAU in a regenerative adult vertebrate brain system, we generated a cre/lox-based transgenic model of zebrafish that chronically expresses human TAU(P301L), which is a variant of human TAU protein that forms neurofibrillary tangles in mouse models and humans. Interestingly, we found that although chronic and abundant expression of TAU(P301L) starting from early embryonic development led to hyperphosphorylation, TAU(P301L) did not form oligomers and neurofibrillary tangles, and did not cause elevated apoptosis and microglial activation, which are classical symptoms of tauopathies in mammals...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29021367/disruption-of-trim9-function-abrogates-macrophage-motility-in-vivo
#20
Debra A Tokarz, Amy K Heffelfinger, Dereje D Jima, Jamie Gerlach, Radhika N Shah, Ivan Rodriguez-Nunez, Amanda N Kortum, Ashley A Fletcher, Shila K Nordone, J McHugh Law, Steffen Heber, Jeffrey A Yoder
The vertebrate immune response comprises multiple molecular and cellular components that interface to provide defense against pathogens. Because of the dynamic complexity of the immune system and its interdependent innate and adaptive functionality, an understanding of the whole-organism response to pathogen exposure remains unresolved. Zebrafish larvae provide a unique model for overcoming this obstacle, because larvae are protected against pathogens while lacking a functional adaptive immune system during the first few weeks of life...
October 11, 2017: Journal of Leukocyte Biology
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