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Joanna E Kowalczyk, Claire Khosravi, Samuel Purvine, Alice Dohnalkova, William B Chrisler, Galya Orr, Errol Robinson, Erika Zink, Ad Wiebenga, Mao Peng, Evy Battaglia, Scott Baker, Ronald P de Vries
In A. niger, two transcription factors, AraR and XlnR, regulate the production of enzymes involved in degradation of arabinoxylan and catabolism of the released L-arabinose and D-xylose. Deletion of both araR and xlnR in leads to reduced production of (hemi)cellulolytic enzymes and reduced growth on arabinan, arabinogalactan and xylan. In this study we investigated the colonization and degradation of wheat bran by the A. niger reference strain CBS 137562 and araR/xlnR regulatory mutants using high-resolution microscopy and exo-proteomics...
October 13, 2017: Environmental Microbiology
Oscar Dias, Thiago O Basso, Isabel Rocha, Eugénio C Ferreira, Andreas K Gombert
In the original publication of the article, the below mentioned errors have appeared. The correct text is provided in this erratum, In the abstract section, the sentence "This dataset serve" should be replaced as "This dataset serves". Also, the reference "Basso TO, Gomes FS, Lopes ML, et al (2014) Homo- and heterofermentative lactobacilli differently affect sugarcane-based fuel ethanol fermentation. Antonie Van Leeuwenhoek 105:169-177. doi: 10.1007/s10482-013-0063-6 " should be replaced as "Basso TO, Dario MG, Tonso A, Stambuk BU, Gombert AK (2010) Insufficient uracil supply in fully aerobic chemostat cultures of Saccharomyces cerevisiae leads to respiro-fermentative metabolism and double nutrient-limitation...
October 12, 2017: Antonie Van Leeuwenhoek
Effat Sadat Merghati-Khoei, Zahed Rezaei, Davood Shojaei-Zadeh, Nammam Ali Azadi, Shahnaz Rimaz, Alireza Bayat, Saeedeh Moayedi-Nia, Sanaz Omati, Farahnaz Salimi, Jeffrey Korte, Therese Killeen, Minoo Mohraz
BACKGROUND: We aimed to investigate risky sexual behaviors (RSBs) and condom use barriers in Iranian men with substance use disorders (SUDs). METHODS: Of the total 1800 outpatient drug free (ODF) and methadone maintenance treatment program (MMTP) active centers in Tehran, Iran, six were selected to participate in the current study. Data were collected (n = 300 men) using three questionnaires including a demographic questionnaire, the Risky Sexual Behavior Questionnaire (RSBQ), and the Condom Barriers Scale (CBS)...
January 2017: Addiction & Health
Dongbao Chen, Lin Feng, Jennifer K Hodges, Thomas J Lechuga, Honghai Zhang
Endogenous hydrogen sulfide (H2S), mainly synthesized by cystathionine β-synthase (CBS) and cystathionineγ-lyase (CTH), has been implicated in regulating placental angiogenesis; however, the underlying mechanisms are unknown. This study was to test a hypothesis that trophoblasts synthesize H2S to promote placental angiogenesis. Human choriocarcinoma-derived BeWo cells expressed both CBS and CTH proteins while the first trimester villous trophoblast-originated HTR-8/SVneo cells expressed CTH protein only. The H2S producing ability of BeWo cells was significantly inhibited by either inhibitors of CBS (CHH) or CTH (BCA) and that in HTR-8/SVneo cells was inhibited by CHH only...
August 28, 2017: Biology of Reproduction
Wilfredo Oliva-Olivera, Leticia Coín-Aragüez, Said Lhamyani, Julián Salas, Adiana-Mariel Gentile, Silvana-Yanina Romero-Zerbo, Hatem Zayed, J F Valderrama, Francisco José Tinahones, Rajaa E L Bekay
Adipose tissue-derived multipotent mesenchymal cells (ASCs) participate in the formation of blood vessels under hypoxic conditions. It is probable that the susceptibility of ASCs to the influence of age and aging-associated pathologies compromises their therapeutic effectiveness depending on the adipose tissue (AT) depot. Our aim was to examine the neovascular potential under hypoxic conditions of ASCs-derived from thymic (thymASCs) and subcutaneous (subASCs) AT from 39 subjects with and without type 2 diabetes mellitus (T2DM) and of different ages who were undergoing coronary bypass surgery (CBS)...
October 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
Jun Zheng, Yun-Feng Lu, Xiao-Jing Liu, Feng-Li Hui
Three strains representing a novel species of yeast were isolated from samples of rotting wood collected from Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that the novel species is a member of the genus Cyberlindnera, although the formation of ascospores was not observed. The novel species was related most closely to the type strain of the species Candida pattaniensis, but they had a 0...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Pamela Kuflik Horowitz, Annarosa Anat Shemesh, Tuvia Horev
BACKGROUND: Israeli policymakers have expressed serious concerns about being able to meet the growing demand for physician services. For this reason, the Israel Ministry of Health (MoH) undertook studies based on 2008 and then 2012 data to obtain an accurate assessment of the size, specialty mix, demographic and geographic composition of the physician workforce. This paper highlights the findings from these studies about the number and percentage of licensed physicians in Israel who were not available, were only partially available, or were about to leave the Israeli healthcare workforce...
May 30, 2017: Israel Journal of Health Policy Research
Yuki Furutani, Yuki Dohara, Shinji Kudo, Jun-Ichiro Hayashi, Koyo Norinaga
The theoretical aspects of the development of a chemical kinetic model for guaiacol and catechol pyrolysis are presented to describe the pyrolysis behaviors of the individual lignin-derived components. The possible pyrolysis pathways involving both unimolecular and bimolecular decomposition were investigated by the potential energy surfaces (PES) calculated at CBS-QB3 level. The high-pressure limiting rate constants of each elementary reaction step were evaluated based on the transition state theory (TST) to determine the dominant pyrolysis pathways...
October 10, 2017: Journal of Physical Chemistry. A
Sirous Yourdkhani, Mirosław Jabłoński, Jorge Echeverría
We report in this work a combined structural and state-of-the-art computational study of homopolar P-HH-P intermolecular contacts. Database surveys have shown the abundance of such surprisingly unexplored contacts, which are usually accompanied by other weak interactions in the solid state. By means of a detailed theoretical study utilizing SAPT(DFT), MP2, SCS-MP2, MP2C and CCSD(T) methods and both aug-cc-pVXZ and aug-cc-pCVXZ (X = D, T, Q, 5) basis sets as well as extrapolation to the CBS limit, we have shown that P-HH-P contacts are indeed attractive and considerably strong...
October 10, 2017: Physical Chemistry Chemical Physics: PCCP
Pavol Sulo, Dana Szabóová, Peter Bielik, Silvia Poláková, Katarína Šoltys, Katarína Jatzová, Tomáš Szemes
The yeast Saccharomyces are widely used to test ecological and evolutionary hypotheses. A large number of nuclear genomic DNA sequences are available, but mitochondrial genomic data are insufficient. We completed mitochondrial DNA (mtDNA) sequencing from Illumina MiSeq reads for all Saccharomyces species. All are circularly mapped molecules decreasing in size with phylogenetic distance from Saccharomyces cerevisiae but with similar gene content including regulatory and selfish elements like origins of replication, introns, free-standing open reading frames or GC clusters...
June 15, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Justen J Poole, German A Gomez-Rios, Ezel Boyaci, Nathaly Reyes-Garcés, Janusz Pawliszyn
The widespread use of pharmaceuticals in both human and animal populations and the resultant contamination of surface waters from the outflow of water treatment facilities is an issue of growing concern. This has raised the need for analytical methods that can both perform rapid sample analysis and overcome the limitations of conventional analysis procedures, such as multistep workflows and tedious procedures. Coated blade spray (CBS) is a solid-phase-microextraction-based technique that enables the direct-to-mass-spectrometry analysis of extracted compounds via the use of limited organic solvent to desorb analytes and perform electrospray ionization...
October 9, 2017: Environmental Science & Technology
Mujtaba Baqar, Yumna Sadef, Sajid Rashid Ahmad, Adeel Mahmood, Abdul Qadir, Iqra Aslam, Jun Li, Gan Zhang
Ecological risk assessment, spatio-temporal variation, and source apportionment of polychlorinated biphenyls (PCBs) were studied in surface sediments and water from River Ravi and its three northern tributaries (Nullah Deg, Nullah Basantar, and Nullah Bein) in Pakistan. In total, 35 PCB congeners were analyzed along 27 sampling stations in pre-monsoon and post-monsoon seasons. The ∑35PCB concentration ranged from 1.06 to 95.76 ng/g (dw) in sediments and 1.94 to 11.66 ng/L in water samples, with hexa-CBs and tetra-CBs as most dominant homologs in sediments and water matrixes, respectively...
October 7, 2017: Environmental Science and Pollution Research International
Alexander A Baykov, Victor A Anashkin, Anu Salminen, Reijo Lahti
Inorganic pyrophosphatases (PPases) convert pyrophosphate to phosphate and are present in all cell types. Soluble PPases belong to three non-homologous families, of which Family II is found in approximately a quarter of prokaryotic organisms, often pathogenic ones. Each subunit of dimeric canonical Family II PPases is formed by two domains connected by a flexible linker, with the active site located between the domains. These enzymes require both magnesium and a transition metal ion (manganese or cobalt) for maximal activity and are the most active (kcat ≈ 10(4) s(-1) ) among all PPase types...
October 7, 2017: FEBS Letters
Antonia Katsouda, Csaba Szabo, Andreas Papapetropoulos
Hydrogen sulfide (H2S) is an endogenously produced signaling molecule synthesized by cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST). Given that H2S exerts significant effects on bioenergetics and metabolism, the goal of the current study was to determine the expression of H2S-producing enzymes in adipose tissues in models of obesity and metabolic disruption. Mice fed a western diet expressed lower mRNA levels of all three enzymes in epididymal fat (EWAT), while only CSE and 3-MST were reduced in brown adipose tissue (BAT)...
October 2, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Beibei Tao, Rui Wang, Chen Sun, Yichun Zhu
Hypoxia-induced angiogenesis is a common phenomenon in many physiological and patho-physiological processes. However, the potential differential roles of three hydrogen sulfide producing systems cystathionine γ-lyase (CSE)/H2S, cystathionine β-synthase (CBS)/H2S, and 3-mercaptopyruvate sulfurtransferase (MPST)/H2S in hypoxia-induced angiogenesis are still unknown. We found that minor hypoxia (10% oxygen) significantly increased the migration of vascular endothelial cells while hypoxia (8% oxygen) significantly inhibited cell migration...
2017: Frontiers in Pharmacology
Csaba Szabo, Andreas Papapetropoulos
Over the last decade, hydrogen sulfide (H2S) has emerged as an important endogenous gasotransmitter in mammalian cells and tissues. Similar to the previously characterized gasotransmitters nitric oxide and carbon monoxide, H2S is produced by various enzymatic reactions and regulates a host of physiologic and pathophysiological processes in various cells and tissues. H2S levels are decreased in a number of conditions (e.g., diabetes mellitus, ischemia, and aging) and are increased in other states (e.g., inflammation, critical illness, and cancer)...
October 2017: Pharmacological Reviews
Judith Wurmel, John M Simmie
Theoretical insights into H-abstraction by ozone from saturated species have been virtually nonexistent which is in sharp contrast to reactions with unsaturated species. Our computed rate constants at various levels of theory for reaction with tetrahydrofuran and its methyl derivatives shows that abstraction occurs primarily at the carbons situated beside the heterocyclic oxygen with least likely reaction from the methyl groups. All the methods tested are in broad relative agreement with this conclusion and with recent experiments although they do differ widely as regards their absolute values...
October 4, 2017: Journal of Physical Chemistry. A
Nilgün Yılmaz Demirci, Asiye Uğraş Dikmen, Zarife Abdullayeva, Can Öztürk
INTRODUCTION: It is crucial to diagnose the subtype of lung cancer quickly and accurately for effective therapy. Conventional cytology staining sometimes provides limited information, and additional tissue is often required to diagnose lung cancer. Cell blocks (CB) recovered during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) increases the diagnostic accuracy of the procedure and the likelihood of additional valuable histochemical and immunohistochemical staining...
October 4, 2017: Clinical Respiratory Journal
Monise Helen Masuchi Buscato, Renato Grimaldi, Theo Guenter Kieckbusch
Symmetrical monounsaturated triacylglycerols-also known as SUS (saturated unsaturated saturated triacylglycerols)-are the main triacylglycerols present in cocoa butter (CB), exhibiting decisive influence in the tempering process conditions and sensorial characteristics in chocolate products. CBs with lower amounts of SUS do not crystallize adequately in chocolate processing, generating a low quality product with higher susceptibility to fat bloom formation. In this context, fractions with higher contents of SUS triacylglycerols were obtained by solvent fractionation of cocoa butter, under four operating conditions varying crystallization temperatures (17, 18, 20 and 22 °C) and sample/acetone solvent ratios (1:7 and 1:5, in w/v)...
September 2017: Journal of Food Science and Technology
Ancy D Nalli, Hongxia Wang, Sayak Bhattacharya, Bryan A Blakeney, Karnam S Murthy
Hydrogen sulfide (H2 S) plays an important role in smooth muscle relaxation. Here, we investigated the expression of enzymes in H2 S synthesis and the mechanism regulating colonic smooth muscle function by H2 S. Expression of cystathionine-γ-lyase (CSE), but not cystathionine-β-synthase (CBS), was identified in the colonic smooth muscle of rabbit, mouse, and human. Carbachol (CCh)-induced contraction in rabbit muscle strips and isolated muscle cells was inhibited by l-cysteine (substrate of CSE) and NaHS (an exogenous H2 S donor) in a concentration-dependent fashion...
October 2017: Pharmacology Research & Perspectives
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