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Endometrial mesenchymal stem cell

Carolina Nogueira de Moraes, Leandro Maia, Marianne Camargos Dias, Camila P Freitas Dell'Aqua, Ligia Souza Lima Silveira da Mota, Aspinas Chapwanya, Fernanda da Cruz Landim-Alvarenga, Eunice Oba
Endometrial mesenchymal stem/progenitor cells (eMSCs) are multipotent cells known to modulate the immune system and have clinical application for human and animal health. This makes these bovines cells attractive for dual use as cellular therapy and experimental model. The aim of this study was to isolate, evaluate the differentiation potential, immunophenotypic and immunocytochemistry characteristics, chromosomal stability, cloning efficiency, and cryopreservation response of bovine eMSCs collected in two phases of the estrous cycle...
October 4, 2016: Cell Biology International
Alisa P Domnina, Polina V Novikova, Olga G Lyublinskaya, Valeriy V Zenin, Irina I Fridlyanskaya, Vyacheslav M Mikhailov, Nikolay N Nikolsky
Stem cell transplantation, which is based on the application of mesenchymal stem/stromal cells (MSCs), is a rapidly developing approach to the regenerative therapy of various degenerative disorders characterized by brain and heart failure, as well as skin lesions. In comparison, the use of stem cell transplantations to treat infertility has received less attention. One of the causes of miscarriages and fetal growth delay is the loss of the decidual reaction of endometrial cells. The present study modeled decidualization processes in pseudopregnant rats...
October 2016: Experimental and Therapeutic Medicine
Jianmei Wang, Baohui Ju, Caijun Pan, Yan Gu, Yu Zhang, Li Sun, Bumei Zhang, Yujuan Zhang
AIMS: To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on intrauterine adhesions (IUA). METHODS: BMSCs were isolated and labeled by green fluorescence protein. IUA model was established by mechanical injury. 48 rats were randomly divided into control, IUA model, BMSCs vein injection and BMSCs intrauterine injection groups (n=12 in each group). The third generation of BMSCs was injected through tail vein or intrauterine...
2016: Cellular Physiology and Biochemistry
X-J Liu, X-G Bai, Y-L Teng, L Song, N Lu, R-Q Yang
OBJECTIVE: miRNAs have been recently reported to contribute to the etiology of endometriosis in stem cells. However, the mechanisms remain unclear. The aim of this investigation is to explore the expression of miR-15a-5p and VEGFA in endometrial samples from patients with or without endometriosis. And then examine the regulation by miR-15a-5p on the expression of VEGFA. PATIENTS AND METHODS: Here we collected 31 endometrial samples from patients with or without endometriosis and characterized the miRNAs expression profiles of these two groups...
August 2016: European Review for Medical and Pharmacological Sciences
Dongmei Lai, Ying Guo, Qiuwan Zhang, Yifei Chen, Charlie Xiang
Human endometrial mesenchymal stem cells (EnSCs) derived from menstrual blood are a unique stem cell source. Evidence suggests that EnSCs exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine. However, the potential of EnSCs to differentiate into germline cells in vitro remains unclear. In this study, EnSCs were induced to differentiate into germ cells in a differentiation medium supplemented with 20% human follicular fluid. Our results demonstrated that EnSCs derived from human menstrual blood form oocyte-like cells and express germ cell markers...
September 2, 2016: Acta Biochimica et Biophysica Sinica
Yanling Zhang, Xiaona Lin, Yongdong Dai, Xiaoxiao Hu, Haiyan Zhu, Yinshen Jiang, Songying Zhang
Intrauterine adhesions are common acquired endometrial syndromes secondary to endometrial injury, with limited effective therapies. Recently, several studies have reported that bone marrow stem cells (BMSCs) could repair injured endometrium in animal experiments. However, the role of stem cells in endometrial injury repair and its therapeutic mechanisms remain unclear. Here, we established mouse endometrial injury model and examined the benefit of human endometrial mesenchymal stem cells derived from menstrual blood (MenSCs) in restoration of injured endometrium...
November 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Tianqi Li, Hongjie Pan, Runsheng Li
MicroRNAs (miRNAs) are a group of endogenous, small (about 22 nucleotides) non-coding RNAs which negatively regulate gene expressions. As one of them, miR-204 originates from the sixth intron of the transient receptor potential melastatin 3 (TRPM3) gene. Therefore, expression of miR-204 is under the control of the TRPM3 promoter and regulated by genetic and epigenetic mechanisms. miR-204 has been found to play the important roles in development of eyes and adipogenesis. Its pathological functions have been observed in a few diseases including pulmonary arterial hypertension, diabetes, and various types of cancers...
July 20, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Emma S Lucas, Nigel P Dyer, Katherine Fishwick, Sascha Ott, Jan J Brosens
Endometrial stem-like cells, including mesenchymal stem cells (MSCs) and epithelial progenitor cells, are essential for cyclic regeneration of the endometrium following menstrual shedding. Emerging evidence indicates that endometrial MSCs (eMSCs) constitute a dynamic population of cells that enables the endometrium to adapt in response to a failed pregnancy. Recurrent miscarriage is associated with relative depletion of endometrial eMSCs, which not only curtails the intrinsic ability of the endometrium to adapt to reproductive failure but also compromises endometrial decidualization, an obligatory transformation process for embryo implantation...
November 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Francisca Alcayaga-Miranda, Paz L González, Alejandra Lopez-Verrilli, Manuel Varas-Godoy, Carolina Aguila-Díaz, Luis Contreras, Maroun Khoury
Mesenchymal stem cells (MSCs) secrete exosomes that are capable of modifying the tumor environment through different mechanisms including changes in the cancer-cell secretome. This activity depends on their cargo content that is largely defined by their cellular origin. Endometrial cells are fine regulators of the angiogenic process during the menstrual cycle that includes an angiostatic condition that is associated with the end of the cycle. Hence, we studied the angiogenic activity of menstrual stem cells (MenSCs)-secreted exosomes on prostate PC3 tumor cells...
June 6, 2016: Oncotarget
Stuart J Emmerson, Caroline E Gargett
Pelvic organ prolapse (POP) occurs when the pelvic organs (bladder, bowel or uterus) herniate into the vagina, causing incontinence, voiding, bowel and sexual dysfunction, negatively impacting upon a woman's quality of life. POP affects 25% of all women and results from childbirth injury. For 19% of all women, surgical reconstructive surgery is required for treatment, often augmented with surgical mesh. The surgical treatment fails in up to 30% of cases or results in adverse effects, such as pain and mesh erosion into the bladder, bowel or vagina...
May 26, 2016: World Journal of Stem Cells
Saeedeh Darzi, Jerome A Werkmeister, James A Deane, Caroline E Gargett
UNLABELLED: SummaryHuman endometrium is a highly regenerative tissue, undergoing more than 400 cycles of proliferation, differentiation, and shedding during a woman's reproductive life. Adult stem cells, including mesenchymal stem/stromal cells (MSCs), are likely responsible for the immense cellular turnover in human endometrium. The unique properties of MSCs, including high proliferative ability, self-renewal, differentiation to mesodermal lineages, secretion of angiogenic factors, and many other growth-promoting factors make them useful candidates for cellular therapy and tissue engineering...
September 2016: Stem Cells Translational Medicine
Samantha Kaufhold, Hermes Garbán, Benjamin Bonavida
The transcription factor Yin Yang 1 (YY1) is frequently overexpressed in cancerous tissues compared to normal tissues and has regulatory roles in cell proliferation, cell viability, epithelial-mesenchymal transition, metastasis and drug/immune resistance. YY1 shares many properties with cancer stem cells (CSCs) that drive tumorigenesis, metastasis and drug resistance and are regulated by overexpression of certain transcription factors, including SOX2, OCT4 (POU5F1), BMI1 and NANOG. Based on these similarities, it was expected that YY1 expression would be associated with SOX2, OCT4, BMI1, and NANOG's expressions and activities...
2016: Journal of Experimental & Clinical Cancer Research: CR
Caroline E Gargett, Shanti Gurung
No abstract text is available yet for this article.
June 2016: Biology of Reproduction
Fatima Barragan, Juan C Irwin, Shaina Balayan, David W Erikson, Joseph C Chen, Sahar Houshdaran, Terhi T Piltonen, Trimble L B Spitzer, Ashley George, Joseph T Rabban, Camran Nezhat, Linda C Giudice
Human endometrium undergoes cyclic regeneration involving stem/progenitor cells, but the role of resident endometrial mesenchymal stem cells (eMSC) as progenitors of endometrial stromal fibroblasts (eSF) has not been definitively demonstrated. In endometriosis, eSF display progesterone (P4) resistance with impaired decidualization in vivo and in vitro. To investigate eMSC as precursors of eSF and whether endometriosis P4 resistance is inherited from eMSC, we analyzed transcriptomes of eutopic endometrium eMSC and eSF isolated by fluorescence-activated cell sorting (FACS) from endometriosis (eMSCendo, eSFendo) and controls (eMSCcontrol, eSFcontrol) and their derived primary cultures...
May 2016: Biology of Reproduction
Jing Li, Yongdong Dai, Haiyan Zhu, Yinshen Jiang, Songying Zhang
STUDY QUESTION: Are endometriotic mesenchymal stem cells (Ecto-MSCs) involved in the fibrosis of ovarian endometrioma? SUMMARY ANSWER: Ecto-MSCs enhanced the fibrotic behavior of stromal cells in ovarian endometrioma through the Wnt/β-catenin pathway by paracrine production of transforming growth factor-β1 (TGF-β1) and Wnt1. WHAT IS KNOWN ALREADY: Endometriosis is characterized by ectopic outgrowth of endometrial stroma and glands surrounded by dense fibrous tissues...
June 2016: Human Reproduction
Somayeh Ebrahimi-Barough, Elham Hoveizi, Meysam Yazdankhah, Jafar Ai, Mehrdad Khakbiz, Faezeh Faghihi, Roksana Tajerian, Neda Bayat
Small molecules as useful chemical tools can affect cell differentiation and even change cell fate. It is demonstrated that LY294002, a small molecule inhibitor of phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway, can inhibit proliferation and promote neuronal differentiation of mesenchymal stem cells (MSCs). The purpose of this study was to investigate the differentiation effect of Ly294002 small molecule on the human endometrial stem cells (hEnSCs) into motor neuron-like cells on polycaprolactone (PCL)/collagen scaffolds...
March 18, 2016: Molecular Neurobiology
A R Koippallil Gopalakrishnan, H Pandit, S M Metkari, N Warty, T Madan
This study was undertaken to study the efficiency of Adsflt-1 engineered human eutopic mesenchymal stem cells (MSCs) secreting anti-angiogenic sFlt-1 as a targeted cell-based therapy for endometriosis (EM). Eutopic MSCs were transduced with Adsflt-1/AdV0 viral vectors and were evaluated for expression and secretion of sFlt-1. EM was created in NOD/SCID mice using subcutaneous implantation techniques. Four doses of 10(6) MSC-Adsflt-1/MSC-AdV0 were administered to the model and therapeutic anti-angiogenic ability was analyzed by lesion size measurement, microvessel density, immunohistochemistry and real-time reverse transcriptase-PCR analysis...
July 2016: Gene Therapy
Agata Łuczak, Anna Supernat, Sylwia Łapińska-Szumczyk, Daniel Jachimowicz, Hanna Majewska, Jacek Gulczyński, Anna J Żaczek
BACKGROUND: Endometrial cancer (EC) is a hormone-related disease, showing highly diverse features of ER/PR/HER2 status-based molecular subtypes. Long noncoding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) has recently emerged as a key molecule in many cancers, triggering epithelial-mesenchymal transition (EMT)-mediated cancer stem cell (CSC) formation, but little is known about its significance in EC. Thus, we aimed to investigate the clinical significance of HOTAIR itself in different molecular subtypes of EC and possible links between HOTAIR, EMT and CSC-related markers...
2016: International Journal of Biological Markers
Julia C Eggers, Valentina Martino, Rolland Reinbold, Sebastian D Schäfer, Ludwig Kiesel, Anna Starzinski-Powitz, Andreas N Schüring, Björn Kemper, Burkhard Greve, Martin Götte
Endometriosis is characterized by growth of endometrial tissue at ectopic locations. Down-regulation of microRNA miR-200b is observed in endometriosis and malignant disease, driving tumour cells towards an invasive state by enhancing epithelial-to-mesenchymal transition (EMT). miR-200b up-regulation may inhibit EMT and invasive growth in endometriosis. To study its functional impact on the immortalized endometriotic cell line 12Z, the stromal cell line ST-T1b, and primary endometriotic stroma cells, a transient transfection approach with microRNA precursors was employed...
April 2016: Reproductive Biomedicine Online
Vladislav S Baranov, Tatyana E Ivaschenko, Maria I Yarmolinskaya
Endometriosis (EM) and uterine leiomyoma (UL) are two most frequent benign tumors of monoclonal origin affecting about 30% of all women in their reproductive age. Modern molecular technologies have made a tremendous impact in understanding both disorders. Here is the first comparative analysis of molecular mechanisms underlying development of EM and UL as it looks from the platform of systems genetics. Similarities and differences of EM and UL at their incipient stages are enlightened with special emphasis on their gene networks, gene expression, and epigenetic regulation, of pathologic development...
April 2016: Systems Biology in Reproductive Medicine
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