keyword
https://read.qxmd.com/read/38531608/human-endometrium-derived-mesenchymal-stem-cells-with-aberrant-nod1-expression-are-associated-with-ectopic-endometrial-lesion-formation
#1
JOURNAL ARTICLE
Chunmei Li, Suiyu Luo, Ai Guo, Ying Su, Yuhui Zhang, Yan Song, Mei Liu, Lu Wang, Yuanyuan Zhang
Nucleotide-binding oligomerization domain 1 (NOD1), a cytosolic pattern recognition receptor protein, plays a crucial role in innate immune responses. However, the functional expression of NOD1 in mesenchymal stem cells (MSCs) derived from endometriosis remains unclear. The aim of this study was to explore the functions of NOD1 in ectopic endometrial lesions. Tissues and MSCs were isolated from both normal endometrium and endometriosis. Immunohistochemistry and real time quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of NOD1 in the tissues/MSCs...
March 27, 2024: International Journal of Stem Cells
https://read.qxmd.com/read/38525237/intrauterine-injection-of-bioengineered-hydrogel-loaded-exosomes-derived-from-hucm-stem-cells-and-spermidine-prominently-augments-the-pregnancy-rate-in-thin-endometrium-rats
#2
JOURNAL ARTICLE
Xiuying Lin, Yanqiu Fang, Xuguang Mi, Jianhua Fu, Shiling Chen, Mengxue Wu, Ningyi Jin
The endometrium is essential to the development of embryos and pregnancy. Human umbilical cord mesenchymal stem cells (HUCMSCs) are promising stem cell sources. HUCMSCs self-renew quickly and are painless to collect. Spermidine is an inherent polyamine needed for cellular and molecular processes that regulate physiology and function. HUCMSCs and spermidine (SN) may heal intrauterine adhesions. HUCMSCs were investigated for endometrial repair in rats. Composite hydrogels are used for medical exosome implantation, including their materials, properties, and embedding procedures...
December 2024: Regenerative Therapy
https://read.qxmd.com/read/38499950/combination-therapy-of-bone-marrow-mesenchymal-stem-cell-transplantation-and-electroacupuncture-for-the-repair-of-intrauterine-adhesions-in-rats-mechanisms-and-functional-recovery
#3
JOURNAL ARTICLE
Zhaoxian Wang, Liangjun Xia, Jie Cheng, Jingyu Liu, Qian Zhu, Chuting Cui, Junwei Li, Yueying Huang, Jie Shen, Youbing Xia
Transplantation of bone marrow mesenchymal stem cells (BMSCs) has demonstrated promising clinical utility in the treatment of endometrial injury and the restoration of fertility. However, since the efficacy of BMSCs after transplantation is not stable, it is very important to find effective ways to enhance the utilisation of BMSCs. Electroacupuncture (EA) has some positive effects on the chemotaxis of stem cells and diseases related to uterine injury. In this study, we established the intrauterine adhesion (IUA) model of the Sprague-Dawley rat using lipopolysaccharide infection and mechanical scratching...
March 18, 2024: Reproductive Sciences
https://read.qxmd.com/read/38430847/ex-vivo-expansion-of-hematopoietic-stem-cells-in-two-three-dimensional-co-cultures-with-various-source-of-stromal-cells
#4
JOURNAL ARTICLE
Nasim Mayeli Fereydani, Hamid Galehdari, Elham Hoveizi, Arash Alghasi, Monireh Ajami
The ex vivo expansion of hematopoietic stem cells, with both high quantities and quality, is considered a paramount issue in cell and gene therapy for hematological diseases. Complex interactions between the bone marrow microenvironment and hematopoietic stem cells reveal the importance of using 2D and 3D coculture as a physiological system simulator in the proliferation, differentiation, and homeostasis of HSCs. Herein, the capacity of mesenchymal stem cells derived from different sources to support the expansion and maintenance of HSPC was compared with each other...
February 19, 2024: Tissue & Cell
https://read.qxmd.com/read/38417279/uterine-wound-healing-after-caesarean-section-a-systematic-review
#5
REVIEW
E Debras, P Capmas, C Maudot, P Chavatte-Palmer
The rate of caesarean section (CS) is increasing worldwide. Defects in uterine healing have a major gynaecological and obstetric impact (uterine rupture, caesarean scar defect, caesarean scar pregnancy, placenta accreta spectrum). The complex process of cellular uterine healing after surgery, and specifically after CS, remains poorly understood in contrast to skin wound healing. This literature review on uterine wound healing was mainly based on histological observations, particularly after CS. The primary objective of the review was to examine the effects of CS on uterine tissue at the cellular level, based on histological observations...
February 27, 2024: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://read.qxmd.com/read/38406291/hypoxic-bone-marrow-mesenchymal-stem-cell-exosomes-promote-angiogenesis-and-enhance-endometrial-injury-repair-through-the-mir-424-5p-mediated-dll4-notch-signaling-pathway
#6
JOURNAL ARTICLE
Zhenghua Xiong, Yong Hu, Min Jiang, Beibei Liu, Wenjiao Jin, Huiqin Chen, Linjuan Yang, Xuesong Han
BACKGROUND: Currently, bone marrow mesenchymal stem cells (BMSCs) have been reported to promote endometrial regeneration in rat models of mechanically injury-induced uterine adhesions (IUAs), but the therapeutic effects and mechanisms of hypoxic BMSC-derived exosomes on IUAs have not been elucidated. OBJECTIVE: To investigate the potential mechanism by which the BMSCS-derived exosomal miR-424-5p regulates IUA angiogenesis through the DLL4/Notch signaling pathway under hypoxic conditions and promotes endometrial injury repair...
2024: PeerJ
https://read.qxmd.com/read/38396297/menstrual-blood-derived-endometrial-stem-cell-transplantation-improves-male-reproductive-dysfunction-in-t1d-mice-by-enhancing-antioxidative-capacity
#7
JOURNAL ARTICLE
Yilin Lu, Ruihong Liu, Xingpeng Kang, Shenghui Zhang, Yuliang Sun, Wenqiang Fan, Hongbin Cheng, Yanli Liu, Juntang Lin
Diabetes is known to negatively affect male reproduction. Recent clinical results have confirmed that mesenchymal stem cell (MSC)-based therapies are safe and effective for the treatment of diabetes. However, the effect and potential mechanism through which MSC transplantation improves diabetes-derived male reproductive dysfunction are still unknown. In the present study, we first established a male T1D mouse model through intraperitoneal injection of streptozotocin for five consecutive days. Subsequently, we evaluated the blood glucose levels, fertility, and histology and immunology of the pancreas, testes, and penis of T1D mice with or without transplantation of menstrual blood-derived endometrial stem cells (MenSCs) or umbilical cord mesenchymal stem cells (UCMSCs)...
February 23, 2024: Reproductive Sciences
https://read.qxmd.com/read/38386960/the-pathogenesis-of-endometriosis-and-adenomyosis-insights-from-single-cell-rna-sequencing
#8
JOURNAL ARTICLE
Qiutong Li, Jinghua Shi, Dai Yi, Xiaoyan Li, Zhiyue Gu, Hailan Yan, Jinhua Leng
Endometriosis and adenomyosis are two similar gynecological diseases that are characterized by ectopic implantation and the growth of the endometrial tissue. Previous studies have reported that they share a common pathophysiology in some respects, such as a similar cellular composition and resistance to the progestogen of lesions, but their underlying mechanisms remain elusive. Emerging single-cell RNA sequencing (scRNA-seq) technologies allow for the dissection of single-cell transcriptome mapping to reveal the etiology of diseases at the level of the individual cell...
February 22, 2024: Biology of Reproduction
https://read.qxmd.com/read/38363406/unveiling-the-protective-effects-of-bmscs-anti-mir-124-3p-exosomes-on-lps-induced-endometrial-injury
#9
JOURNAL ARTICLE
Yihong Chen, Shan Zheng, Xiumei Zhao, Yi Zhang, Suchai Yu, Juanbing Wei
Researchers have reported that miR-124-3p is highly expressed in patients with chronic endometritis. However, the underlying mechanism of miR-124-3p in the development of endometritis remains unclear. This study constructed an in vitro endometrial cell injury model by treating HEECs with 2 μg/mL LPS for 48 h. Then, 1 mg/kg LPS was injected into both sides of the mouse uterus to construct an in vivo endometrial injury model. The expression of miR-124-3p in human endometrial epithelial cells (HEECs) was assessed using RT‒qPCR...
February 16, 2024: Functional & Integrative Genomics
https://read.qxmd.com/read/38353345/histological-study-of-telocytes-in-mice-intrauterine-adhesion-model-and-their-positive-effect-on-mesenchymal-stem-cells-in-vitro
#10
JOURNAL ARTICLE
Xiaojiao Wei, Hui Li, Tianquan Chen, Xiaojun Yang
Intrauterine adhesions (IUA), the main cause of secondary infertility in women, result from irreversible fibrotic repair of the endometrium due to inflammation or human factors, accompanied by disruptions in the repair function of endometrial stem cells. This significantly impacts the physical and mental health of women in their childbearing years. Telocytes (TCs), a distinctive type of interstitial cells found in various tissues and organs, play diverse repair functions due to their unique spatial structure...
February 14, 2024: Cell Biology International
https://read.qxmd.com/read/38347646/endometrial-mesenchymal-stromal-stem-cells-improve-regeneration-of-injured-endometrium-in-mice
#11
JOURNAL ARTICLE
Tianqi Li, Rachel W S Chan, Raymond H W Li, Ernest H Y Ng, Songying Zhang, William S B Yeung
BACKGROUND: The monthly regeneration of human endometrial tissue is maintained by the presence of human endometrial mesenchymal stromal/stem cells (eMSC), a cell population co-expressing the perivascular markers CD140b and CD146. Endometrial regeneration is impaired in the presence of intrauterine adhesions, leading to infertility, recurrent pregnancy loss and placental abnormalities. Several types of somatic stem cells have been used to repair the damaged endometrium in animal models, reporting successful pregnancy...
February 13, 2024: Biological Research
https://read.qxmd.com/read/38338745/long-term-cryopreservation-may-cause-genomic-instability-and-the-premature-senescence-of-cells
#12
JOURNAL ARTICLE
Mariia Shorokhova, Natalia Pugovkina, Victoria Zemelko, Olga Lyublinskaya, Tatiana Grinchuk
Cryopreservation is an essential step for utilizing various cell types for biological research and medical purposes. At the same time, there is a lack of data on the effect of cryopreservation, especially when prolonged, on the karyotype of cells. In the present work, we analyzed the genetic stability of cells subjected to a cryopreservation procedure. The objects were immortalized Chinese hamster lung fibroblasts (CHL V-79 RJK line) and human endometrial mesenchymal stem/stromal cells (eMSCs). We showed that short-term cryopreservation in liquid nitrogen for up to 6 months did not affect the karyotype stability of CHL V-79 RJK and eMSCs...
January 25, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38331133/minimally-invasive-delivery-of-human-umbilical-cord-derived-mesenchymal-stem-cells-by-an-injectable-hydrogel-via-diels-alder-click-reaction-for-the-treatment-of-intrauterine-adhesions
#13
JOURNAL ARTICLE
Sentao Hu, Yangyang Dai, Liaobing Xin, Xiaowen Zheng, Zi Ye, Songying Zhang, Lie Ma
Intrauterine adhesions (IUA) are the most common cause of uterine infertility, and conventional treatments have not consistently achieved satisfactory pregnancy rates. Stem cell therapy shows promising potential for the clinical treatment of IUA. Although various advanced biomaterials have been designed for delivering stem cells to the uterine cavity, there remain significant challenges, particularly in devising therapeutic strategies for clinical application that minimize surgical incisions and conform to the intricate structure of uterine cavity...
February 6, 2024: Acta Biomaterialia
https://read.qxmd.com/read/38315148/incorporation-of-decidual-stromal-cells-derived-exosomes-in-sodium-alginate-hydrogel-as-an-innovative-therapeutic-strategy-for-advancing-endometrial-regeneration-and-reinstating-fertility
#14
JOURNAL ARTICLE
Yuxiang Liang, Qizhi Shuai, Xiao Zhang, Shanshan Jin, Yuqian Guo, Zhaowei Yu, Xinrui Xu, Ruifang Ao, Zhiwei Peng, Huimin Lv, Sheng He, Chunfang Wang, Guohua Song, Zhizhen Liu, Hong Zhao, Qilong Feng, Ruochen Du, Bin Zheng, Zhaoyang Chen, Jun Xie
Intrauterine adhesion (IUA) stands as a prevalent medical condition characterized by endometrial fibrosis and scar tissue formation within the uterine cavity, resulting in infertility and, in severe cases, recurrent miscarriages. Cell therapy, especially with stem cells, offers an alternative to surgery, but concerns about uncontrolled differentiation and tumorigenicity limit its use. Exosomes, more stable and immunogenicity-reduced than parent cells, have emerged as a promising avenue for IUA treatment. In this study, a novel approach has been proposed wherein exosomes originating from decidual stromal cells are encapsulated within sodium alginate hydrogel (SAH) scaffolds to repair endometrial damage and restore fertility in a mouse IUA model...
February 5, 2024: Advanced Healthcare Materials
https://read.qxmd.com/read/38294669/a-novel-method-to-repair-thin-endometrium-and-restore-fertility-based-on-menstruation-derived-stem-cell
#15
JOURNAL ARTICLE
Kai Chen, Huiru Wang, Xin Zhao, Jingxin Wang, Qi Jin, Xianhong Tong, Shengxia Zheng
Thin endometrium (TE), which mainly occurs as a result of severe damage to the endometrial basalis, is one of the prominent etiologies of menstrual abnormalities, infertility, and recurrent miscarriage in women. Previous studies have demonstrated that mesenchymal stem cells (MSCs) are considered ideal cells with multipotency for regenerative medicine and exhibit therapeutic effects on TE through their cellular secretions. However, there is limited research on strategies to enhance MSC secretion to improve their therapeutic efficacy...
January 31, 2024: Reproductive Sciences
https://read.qxmd.com/read/38241636/mesenchymal-stem-cell-derived-exosomes-repair-uterine-injury-by-targeting-transforming-growth-factor-%C3%AE-signaling
#16
JOURNAL ARTICLE
Huidong Liu, Xiao Zhang, Mengtong Zhang, Sichen Zhang, Jin Li, Yingmin Zhang, Qingyu Wang, Jian Ping Cai, Ke Cheng, Shaowei Wang
Intrauterine adhesions (IUA) refer to adhesions within the uterine cavity and cervix caused by injuries from uterine surgery. They are a significant cause of female infertility. Exosomes derived from mesenchymal stem cells (MSCs) play an active role in the treatment of IUA. However, the mechanism by which they reduce fibrosis in the damaged endometrium remains unclear. In this paper, we demonstrate that exosomes derived from placental mesenchymal stem cells (PMSCs) can restore uterine functions and improve the fertility rate of injured animals...
January 19, 2024: ACS Nano
https://read.qxmd.com/read/38235889/the-effect-of-bmscs-on-implantation-mechanisms-and-pregnancy-process-in-the-experimental-asherman-model
#17
JOURNAL ARTICLE
U Sarı, Z Kamalak, S M Özçelik, I Özdemir
OBJECTIVE: In this study, we aimed to investigate the role of bone marrow-derived mesenchymal stem cells (BMSC) on the molecular mechanisms involved in the removal of endometrial adhesions and the implantation process in the experimentally-created Asherman syndrome model in rats. MATERIALS AND METHODS: An experimental model chemical agent was used. Culture medium (CM), only BMSC, 48-hour culture medium (Niche), and BMSC+ niche were used as therapy. Each group was divided into two subgroups, and changes in the endometrial tissue were investigated by histochemical and immunohistochemical staining...
January 2024: European Review for Medical and Pharmacological Sciences
https://read.qxmd.com/read/38224009/novel-therapeutic-targets-including-igfbp3-of-umbilical-cord-mesenchymal-stem-cell-conditioned-medium-in-intrauterine-adhesion
#18
JOURNAL ARTICLE
Yuan Zhu, Mingjie Bao, Ting Wang, Xiaoyan Ai, Dewen Qiu, Changhua Wang
Mesenchymal stem cells play important roles in repairing injured endometrium. However, corresponding molecular targets and potential mechanism of the recipient cells for stem cell therapy in intrauterine adhesion (IUA) is very limited. Methods and Results: In our study, umbilical cord mesenchymal stem cell conditioned medium (UCMSCs-CM) produced positive effects on TGF-β induced IUA cell model. RNA-sequencing was performed on clinical IUA tissues, top40 up-regulated and top20 down-regulated mRNAs were selected and verified using high-throughput (HT) QPCR in both tissues and cell models...
January 15, 2024: Biology Open
https://read.qxmd.com/read/38185531/human-endometrial-regenerative-cells-for-neurological-disorders-hype-or-hope
#19
REVIEW
Javad Momeni, Elnaz Naserzadeh, Ali Sepehrinezhad, Rezan Ashayeri Ahmadabad, Sajad Sahab Negah
Despite enormous efforts, no effective medication has been found to significantly halt or even slow the progression of neurological diseases, such as acquired (e.g., traumatic brain injury, spinal cord injury, etc.) and chronic (e.g., Parkinson's disease, Alzheimer's disease, etc.) central nervous system disorders. So, researchers are looking for alternative therapeutic modalities to manage the disease's symptoms and stop it from worsening. Concerning disease-modifying capabilities, stem cell therapy has emerged as an expanding domain...
January 8, 2024: International Journal of Stem Cells
https://read.qxmd.com/read/38175149/a-novel-conductive-polypyrrole-chitosan-hydrogel-containing-human-endometrial-mesenchymal-stem-cell-derived-exosomes-facilitated-sustained-release-for-cardiac-repair
#20
JOURNAL ARTICLE
Changping Yan, Xinzhu Wang, Qi Wang, Haiyan Li, Huifang Song, Jingli Zhou, Zexu Peng, Wenjuan Yin, Xuemei Fan, Kun Yang, Bingrui Zhou, Yuxiang Liang, Zengyu Jiang, Yuwei Shi, Sanyuan Zhang, Sheng He, Ren-Ke Li, Jun Xie
Myocardial infarction (MI) results in cardiomyocyte necrosis and conductive system damage, leading to sudden cardiac death and heart failure. Studies have shown that conductive biomaterials can restore cardiac conduction, but cannot facilitate tissue regeneration. This study aims to add regenerative capabilities to the conductive biomaterial by incorporating human endometrial mesenchymal stem cell (hEMSC)-derived exosomes (hEMSC-Exo) into poly-pyrrole-chitosan (PPY-CHI), to yield an injectable hydrogel that could effectively treat MI...
January 4, 2024: Advanced Healthcare Materials
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