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Endometrial mesenchymal stem cell

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https://www.readbyqxmd.com/read/29775846/reproductive-stage-and-sex-steroid-hormone-levels-influence-the-expression-of-mesenchymal-stromal-cell-msc-markers-in-the-equine-endometrium
#1
B Elisabeth Rink, Juliane Kuhl, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
Mesenchymal stem or stromal cells (MSCs) play key roles in tissue homeostasis. In the cyclic equine endometrium, this may be regulated by changes in serum concentrations of sex steroid hormones. This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 h and 7days post-partum; n = 5 per stage) and were analyzed by RT-qPCR...
May 4, 2018: Theriogenology
https://www.readbyqxmd.com/read/29715701/application-of-stem-cells-to-infertility-treatment-with-emphasis-on-mesenchymal-stem-cells-and-ovarian-stem-cells
#2
Nahid Lorzadeh, Nastaran Kazemirad
Stem cells are a population of undifferentiated cells that originated from embryonic and fetal stages of development. Stem cells possess the potency to produce cells that ultimately form different tissues and organs. They are considered as potential alternative novel therapeutic agents for patients suffering from infertility due to their unlimited source and high differentiation potency. There are several studies that have shown that different types of stem cells (mesenchymal stem cells, embryonic, endometrial, and ovarian stem cells) can be differentiated into both spermatozoa and oocytes in vitro, proving their potential clinical use in management and cure of infertility issues...
May 1, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29701160/non-pregnant-human-uterus-as-source-of-mesenchymal-stem-cells
#3
Noemi Eiro, Maria Fraile, Jose Schneider, Francisco Vizoso
Stem/progenitor cells for cell therapy and regenerative medicine should ideally be available in large numbers, after having been isolated using minimally invasive or non-invasive methodologies. Also, they should exhibit wide differentiation potential into multiple lineages, as well as capability to be used successfully in autologous or allogeneic transplantation, and all this in accordance with the applicable guidelines of good manufacturing practice. Thus, the identification and characterization of alternative sources of mesenchymal stem cells (MSCs) is of great importance...
April 26, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29700360/endometrial-mesenchymal-stem-stromal-cells-modulate-the-macrophage-response-to-implanted-polyamide-gelatin-composite-mesh-in-immunocompromised-and-immunocompetent-mice
#4
S Darzi, J A Deane, C A Nold, S E Edwards, D J Gough, S Mukherjee, S Gurung, K S Tan, A V Vashi, J A Werkmeister, C E Gargett
The immunomodulatory properties of human endometrial mesenchymal stem cells (eMSC) have not been well characterised. Initial studies showed that eMSC modulated the chronic inflammatory response to a non-degradable polyamide/gelatin mesh in a xenogeneic rat skin wound repair model, but the mechanism remains unclear. In this study, we investigated the immunomodulatory effect of eMSC on the macrophage response to polyamide/gelatin composite mesh in an abdominal subcutaneous wound repair model in C57BL6 immunocompetent and NSG (NOD-Scid-IL2Rgamma null ) immunocompromised mice to determine whether responses differed in the absence of an adaptive immune system and NK cells...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29675492/endometrial-mesenchymal-stem-stromal-cells-in-mature-and-immature-sheep-an-in-vitro-study
#5
Farnaz Ghobadi, Farhad Rahmanifar, Davood Mehrabani, Amin Tamadon, Mehdi Dianatpour, Shahrokh Zare, Iman Razeghian Jahromi
Background: Endometrial mesenchymal stem stromal cells (EnMSCs) are critical for uterine function, repair, and regeneration. Objective: This study introduced isolation technique of EnMSCs and compared the characteristics of EnMSCs in mature and immature ewes. Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes were collected from the slaughterhouse. Endometrial cells were isolated from tissue using cold incubation and then chopping and treating was performed with collagenase type I...
February 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29568895/abnormal-expression-of-fibrosis-markers-estrogen-receptor-%C3%AE-and-stromal-derived-factor%C3%A2-1-chemokine-c%C3%A2-x%C3%A2-c-motif-receptor%C3%A2-4-axis-in-intrauterine-adhesions
#6
Qin Zhou, Xixi Wu, Jianguo Hu, Rui Yuan
Intrauterine adhesions (IUAs) are mainly derived from fibrous tissue formation following endometrial damage. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)α and the stromal derived factor (SDF)‑1/C‑X‑C chemokine receptor type 4 (CXCR‑4) axis are abnormally expressed in IUA endometrium. A total of 76 human endometrial biopsy samples (normal, n=20; mild‑to‑moderate IUAs, n=40; and severe IUAs, n=16) were employed, and Sprague‑Dawley rat IUA models at different time points were constructed...
March 22, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29566849/pathogenesis-of-uterine-adenomyosis-invagination-or-metaplasia
#7
REVIEW
Javier García-Solares, Jacques Donnez, Olivier Donnez, Marie-Madeleine Dolmans
Adenomyosis is a commonly diagnosed estrogen-dependent gynecological disorder that causes pelvic pain, abnormal uterine bleeding, and infertility. Despite its prevalence and severity of symptoms, its pathogenesis and etiology have not yet been elucidated. The aim of this manuscript is to review the different hypotheses on the origin of adenomyotic lesions and the mechanisms involved in the evolution and progression of the disease. Two main theories have been proposed to explain the origin of adenomyosis. The most common suggests involvement of tissue injury and the repair mechanism and claims that adenomyosis results from invagination of the endometrial basalis into the myometrium...
March 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29503126/endometrial-stem-progenitor-cells-and-their-role-in-the-pathogenesis-of-endometriosis
#8
REVIEW
Fiona L Cousins, Dorien F O, Caroline E Gargett
Human endometrium regenerates on a cyclical basis each month, likely mediated by endometrial stem/progenitor cells. Several types of stem/progenitor cells have been identified: CD140b+ CD146+ or SUSD2+ endometrial mesenchymal stem cells (eMSCs), N-cadherin+ endometrial epithelial progenitor cells (eEPs), and side population (SP) cells, a heterogeneous population predominantly comprising endothelial cells. eMSCs reside in a perivascular niche and likely mediate angiogenesis and stromal regeneration. Human eEPs are located in the bases of glands in the basalis and are likely more primitive than SSEA-1+ basalis epithelial cells...
February 8, 2018: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/29482664/human-mesenchymal-stem-cells-in-spheroids-improve-fertility-in-model-animals-with-damaged-endometrium
#9
Alisa Domnina, Polina Novikova, Julia Obidina, Irina Fridlyanskaya, Larisa Alekseenko, Irina Kozhukharova, Olga Lyublinskaya, Valeriy Zenin, Nikolay Nikolsky
BACKGROUND: Asherman's syndrome (AS) is one of the gynecological disorders caused by the destruction of the endometrium. For some cases of AS available surgical methods and hormonal therapy are ineffective. Stem cell transplantation may offer a potential alternative for AS cure. METHODS: Human endometrial mesenchymal stem cells (eMSC) organized in spheroids were transplanted in rats with damaged endometrium modeled on AS. Treatment response was defined as pregnancy outcome and litter size...
February 26, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29482651/effects-of-platelet-rich-plasma-on-the-activity-of-human-menstrual-blood-derived-stromal-cells-in-vitro
#10
Siwen Zhang, Pingping Li, Zhengwei Yuan, Jichun Tan
BACKGROUND: Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. The convenience and non-invasiveness make MenSC a novel cell source for regenerative medicine applications. Platelet-rich plasma (PRP) contains abundant growth factors which are beneficial to wound healing. However, the influence of PRP on MenSCs remains elusive. Here, we evaluated the role of PRP in MenSCs proliferation and assessed the effects of PRP on endometrial receptivity regulation in vitro...
February 26, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29450941/hematogenous-dissemination-of-mesenchymal-stem-cells-from-endometriosis
#11
Fei Li, Myles H Alderman, Aya Tal, Ramanaiah Mamillapalli, Alexis Coolidge, Demetra Hufnagel, Zhihao Wang, Elham Neisani, Stephanie Gidicsin, Graciela Krikun, Hugh S Taylor
Endometriosis is ectopic growth of endometrial tissue traditionally thought to arise through retrograde menstruation. We aimed to determine if cells derived from endometriosis could enter vascular circulation and lead to hematogenous dissemination. Experimental endometriosis was established by transplanting endometrial tissue from DsRed+ mice into the peritoneal cavity of DsRed- mice. Using flow cytometry, we identified DsRed+ cells in blood of animals with endometriosis. The circulating donor cells expressed CXCR4 and mesenchymal stem cell (MSC) biomarkers, but not hematopoietic stem cell markers...
February 16, 2018: Stem Cells
https://www.readbyqxmd.com/read/29433563/therapeutic-effect-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-injured-rat-endometrium-during-its-chronic-phase
#12
Lu Zhang, Ying Li, Chun-Yi Guan, Shi Tian, Xiao-Dan Lv, Jian-Hui Li, Xu Ma, Hong-Fei Xia
BACKGROUND: Repair deficiency after endometrial injury is an important reason for intra-uterine adhesions, amenorrhea, and infertility in females. Bone marrow-derived mesenchymal stem cell (BMSC) transplantation is effective in repairing the damaged endometrium. However, the possibility of using umbilical cord-derived MSCs (UC-MSCs) to treat endometrial injury is rarely reported. METHODS: Ethanol (95%) was injected into rat uterus to establish a model of endometrial injury...
February 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29404863/in-vitro-evidence-that-platelet-rich-plasma-stimulates-cellular-processes-involved-in-endometrial-regeneration
#13
Lusine Aghajanova, Sahar Houshdaran, Shaina Balayan, Evelina Manvelyan, Juan C Irwin, Heather G Huddleston, Linda C Giudice
PURPOSE: The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. METHODS: Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP...
February 5, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29402201/similar-characteristics-of-endometrial-and-endometriotic-epithelial-cells
#14
Lutz Konrad, Judith Gronbach, Fabian Horné, Ezekiel O Mecha, Eniko Berkes, Matthias Frank, Stefan Gattenlöhner, Charles O A Omwandho, Frank Oehmke, Hans-Rudolf Tinneberg
Epithelial-mesenchymal transition (EMT) is characterized by the loss of epithelial and acquisition of mesenchymal cell characteristics. Our aim was to assess the epithelial phenotype in the pathogenesis of endometriosis with epithelial and mesenchymal markers. We used 2 structural (keratin-18, -19 [K18, K19]), 1 membrane-associated (mucin-1 [MUC1]), and 2 mesenchymal proteins (vimentin; zinc finger E-box-binding homeobox 1, [ZEB1]) to compare epithelial and mesenchymal characteristics in eutopic endometrium with the 3 endometriotic entities, peritoneal, ovarian, and deep infiltrating endometriosis (DIE)...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29397307/human-endometrial-stem-cells-high-yield-isolation-and-characterization
#15
Marina V Kovina, Michael E Krasheninnikov, Tatiana G Dyuzheva, Michael I Danilevsky, Ilya D Klabukov, Maxim V Balyasin, Olga K Chivilgina, Alexey V Lyundup
BACKGROUND: Menstrual blood is only recently and still poorly studied, but it is an abundant and noninvasive source of highly proliferative mesenchymal stromal cells (MSCs). However, no appropriate isolation method has been reported due to its high viscosity and high content of clots and desquamated epithelium. METHODS: We studied three different isolation approaches and their combinations: ammonium-containing lysing buffer, distilled water and gradient-density centrifugation...
March 2018: Cytotherapy
https://www.readbyqxmd.com/read/29391698/isolation-culture-characterization-and-osteogenic-differentiation-of-canine-endometrial-mesenchymal-stem-cell
#16
A K Sahoo, J K Das, S Nayak
Aim: In this study, the canine endometrium tissue is characterized for its stem cell properties such as adherence to tissue culture plate (plasticity), short population doubling time, serial clonal passaging, long-term culturing properties, stem cell marker expression, and multilineage differentiation potential. Materials and Methods: The present work describes a novel isolation protocol for obtaining mesenchymal stem cells from the uterine endometrium and is compared with cells derived from umbilical cord matrix as a positive control...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29383153/reciprocal-crosstalk-between-endometrial-carcinoma-and-mesenchymal-stem-cells-via-transforming-growth-factor-%C3%AE-transforming-growth-factor-receptor-and-c-x-c-motif-chemokine-ligand-12-c-x-c-chemokine-receptor-type-4-aggravates-malignant-phenotypes
#17
Dah-Ching Ding, Tang-Yuan Chu, Hwan-Wun Liu
Designated for cyclic shedding, the endometrial stroma is rich in endometrial mesenchymal stem cells (EMSCs) and may play an important role in the development of endometrial carcinoma (EC). This study characterized the crosstalk of EC cells with EMSCs and the resultant effects on malignant phenotypes. The cultured EMSCs expressed CD73, CD90, and CD105, but not CD14, CD19, CD34, CD45, or human leukocyte antigen-antigen D related markers. These EMSCs also showed osteogenic, adipogenic, and chondrogenic differentiation ability...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29241917/pueraria-mirifica-inhibits-17%C3%AE-estradiol-induced-cell-proliferation-of-human-endometrial-mesenchymal-stem-cells
#18
Ta-Chin Lin, Kai-Hung Wang, An-Pei Kao, Kuo-Hsiang Chuang, Tsung-Cheng Kuo
OBJECTIVE: The notion that the human endometrium may contain a population of stem cells has recently been proposed. The mesenchymal stem cells (MSCs) in the endometrium are believed to be responsible for the remarkable regenerative ability of endometrial cells. Estrogens influence the physiological and pathological processes of several hormone-dependent tissues, such as the endometrium. Pueraria mirifica (PM) is a herbal plant that contains several phytoestrogens, including isoflavones, lignans, and coumestans, and is known to exert an estrogenic effect on animal models...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29227245/clearance-of-senescent-decidual-cells-by-uterine-natural-killer-cells-in-cycling-human-endometrium
#19
Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara, Joanne Muter, Emma S Lucas, Taihei Yamada, Laura Woods, Raffaella Lucciola, Yie Hou Lee, Satoru Takeda, Sascha Ott, Myriam Hemberger, Siobhan Quenby, Jan Joris Brosens
In cycling human endometrium, menstruation is followed by rapid estrogen-dependent growth. Upon ovulation, progesterone and rising cellular cAMP levels activate the transcription factor Forkhead box O1 (FOXO1) in endometrial stromal cells (EnSCs), leading to cell cycle exit and differentiation into decidual cells that control embryo implantation. Here we show that FOXO1 also causes acute senescence of a subpopulation of decidualizing EnSCs in an IL-8 dependent manner. Selective depletion or enrichment of this subpopulation revealed that decidual senescence drives the transient inflammatory response associated with endometrial receptivity...
December 11, 2017: ELife
https://www.readbyqxmd.com/read/29215321/mesenchymal-endometrial-stem-stromal-cells-for-hard-tissue-engineering-a-review-of-in-vitro-and-in-vivo-evidence
#20
Fahimeh S Tabatabaei, Jafar Ai
Hard tissues including teeth, bone and cartilage have inability or poor capacity to self-renew, especially in large defects. Therefore, repair of damages in these tissues represents a huge challenge in the medical field today. Hard tissue engineering commonly utilizes different stem cell sources as a promising strategy for treating bone, cartilages and tooth defects or disorders. Decades ago, researchers successfully isolated and identified endometrial mesenchymal stem/stromal cells (EnSCs) and discovered their multidifferentiation potential...
December 2017: Regenerative Medicine
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