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https://www.readbyqxmd.com/read/28444417/identification-of-proteins-differentially-expressed-by-chlamydia-trachomatis-treated-with-chlamydiaphage-capsid-protein-vp1-during-intracellular-growth
#1
Jingyue Ma, Yina Sun, Changgui Sun, Quan Zhou, Manli Qi, Jie Kong, Jing Wang, Yuanjun Liu, Quanzhong Liu
Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases. Our research pertains to the inhibitory effect and molecular mechanism of the chlamydiaphage capsid protein VP1 on the growth of Chlamydia trachomatis. In this research, the capsid protein VP1 of the guinea-pig conjunctivitis chlamydiaphage phiCPG1 was expressed, purified and identified, and then, it was applied to the cultivation of different serovars of Chlamydia trachomatis and Chlamydia psittaci. The inhibitory effect was observed in each serovar of Chlamydia trachomatis (D, E, F, G, H, I, K, and L2) and Chlamydia psittaci inoculated with VP1 protein...
April 25, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28436483/itraq-based-proteomic-analysis-reveals-key-proteins-affecting-muscle-growth-and-lipid-deposition-in-pigs
#2
Zhixiu Wang, Peng Shang, Qinggang Li, Liyuan Wang, Yangzom Chamba, Bo Zhang, Hao Zhang, Changxin Wu
Growth rate and meat quality, two economically important traits in pigs, are controlled by multiple genes and biological pathways. In the present study, we performed a proteomic analysis of longissimus dorsi muscle from six-month-old pigs from two Chinese native mini-type breeds (TP and DSP) and two introduced western breeds (YY and LL) using isobaric tag for relative and absolute quantification (iTRAQ). In total, 4,815 peptides corresponding to 969 proteins were detected. Comparison of expression patterns between TP-DSP and YY-LL revealed 288 differentially expressed proteins (DEPs), of which 169 were up-regulated and 119 were down-regulated...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28433992/the-aachen-minipig-phenotype-genotype-hematological-and-biochemical-characterization-and-comparison-to-the-g%C3%A3-ttingen-minipig
#3
Kerstin Pawlowsky, Lisa Ernst, Julia Steitz, Thaddäus Stopinski, Babette Kögel, Anna Henger, Reinhart Kluge, René Tolba
BACKGROUND: The pig is one of the most frequently used large animal models for biomedical research, especially in the field of translational research and surgical models. While standard livestock breeds are used in short-term and acute studies, minipig breeds are the preferred breeds in long-term and chronic studies due to their limited growth and body weight. OBJECTIVE: In consideration of the 3R principle (refinement, reduction, replacement) and the increasing demand, the aim of this study was to generate a new, robust, non-specific-pathogen-free minipig breed, the Aachen minipig...
April 22, 2017: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/28432701/ovarian-follicle-development-and-genital-tract-characteristics-in-different-birthweight-gilts-at-150%C3%A2-days-of-age
#4
Frcl Almeida, Aln Alvarenga Dias, L P Moreira, Atl Fiúza, H Chiarini-Garcia
In the last decades, selection for improved prolificacy has resulted in higher litter sizes and has thereby increased the proportion of low birthweight (LW) piglets. It is well documented that LW piglets have lower growth performance, muscle accretion and poor carcass quality. However, little is known about the relations of birthweight with subsequent reproductive performance in gilts. This study investigated the effects of birthweight on reproductive tract and ovarian follicle development in 150-day-old gilts...
April 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28430144/a-systems-biology-approach-using-transcriptomic-data-reveals-genes-and-pathways-in-porcine-skeletal-muscle-affected-by-dietary-lysine
#5
Taiji Wang, Jean M Feugang, Mark A Crenshaw, Naresh Regmi, John R Blanton, Shengfa F Liao
Nine crossbred finishing barrows (body weight 94.4 ± 6.7 kg) randomly assigned to three dietary treatments were used to investigate the effects of dietary lysine on muscle growth related metabolic and signaling pathways. Muscle samples were collected from the longissimus dorsi of individual pigs after feeding the lysine-deficient (4.30 g/kg), lysine-adequate (7.10 g/kg), or lysine-excess (9.80 g/kg) diet for five weeks, and the total RNA was extracted afterwards. Affymetrix Porcine Gene 1.0 ST Array was used to quantify the expression levels of 19,211 genes...
April 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28430080/generation-and-characterization-of-inhibitory-antibodies-specific-to-guinea-pig-cxcr1-and-cxcr2
#6
Kento Tanaka, Chigusa Yoshimura, Tetsuo Shiina, Tomoko Terauchi, Tomomi Yoshitomi, Kazuki Hirahara
CXCR1 and CXCR2 are chemokine receptors that have different selectivity of chemokine ligands, but the distinct role of each receptor is not clearly understood. This is due to the absence of specific inhibitors in guinea pigs, which are the appropriate species for investigation of CXCR1 and CXCR2 because of their functional similarity to humans. In this study, we generated and evaluated monoclonal antibodies that specifically bound to guinea pig CXCR1 (gpCXCR1) and guinea pig CXCR2 (gpCXCR2) for acquisition of specific inhibitors...
April 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28430025/synthesis-of-dopamine-in-e-coli-using-plasmid-based-expression-system-and-its-marked-effect-on-host-growth-profiles
#7
Arunangshu Das, Anita Verma, Krishna J Mukherjee
L-Dopa and dopamine are important pathway intermediates towards the synthesis of catecholamine like epinephrine and norepinephrine from amino acid L-tyrosine. Dopamine, secreted from dopaminergic nerve cells, serves as an important neurotransmitter. We report the synthesis of dopamine by extending the aromatic amino acid pathway of E.coli DH5α by expression of 4-hydroxyphenylacetate-3-hydrolase (HpaBC) from E.coli and an engineered dopa decarboxylase (DDC) from pig kidney cell. The activity of HpaBC and DDC require 200 µM iron supplementation and 50 µM vitamin B6 respectively as additives to the growth media...
April 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28428891/dietary-inclusion-effects-of-phytochemicals-as-growth-promoters-in-animal-production
#8
REVIEW
Nidia Vanessa Valenzuela-Grijalva, Araceli Pinelli-Saavedra, Adriana Muhlia-Almazan, David Domínguez-Díaz, Humberto González-Ríos
Growth promoters have been widely used as a strategy to improve productivity, and great benefits have been observed throughout the meat production chain. However, the prohibition of growth promoters in several countries, as well as consumer rejection, has led industry and the academy to search for alternatives. For decades, the inclusion of phytochemicals in animal feed has been proposed as a replacement for traditional growth promoters. However, there are many concerns about the application of phytochemicals and their impact on the various links in the meat production chain (productive performance, carcass and meat quality)...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/28424056/use-of-multi-trait-and-random-regression-models-to-identify-genetic-variation-in-tolerance-to-porcine-reproductive-and-respiratory-syndrome-virus
#9
Graham Lough, Hamed Rashidi, Ilias Kyriazakis, Jack C M Dekkers, Andrew Hess, Melanie Hess, Nader Deeb, Antti Kause, Joan K Lunney, Raymond R R Rowland, Han A Mulder, Andrea Doeschl-Wilson
BACKGROUND: A host can adopt two response strategies to infection: resistance (reduce pathogen load) and tolerance (minimize impact of infection on performance). Both strategies may be under genetic control and could thus be targeted for genetic improvement. Although there is evidence that supports a genetic basis for resistance to porcine reproductive and respiratory syndrome (PRRS), it is not known whether pigs also differ genetically in tolerance. We determined to what extent pigs that have been shown to vary genetically in resistance to PRRS also exhibit genetic variation in tolerance...
April 19, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/28423885/effect-of-fermented-biogas-residue-on-growth-performance-serum-biochemical-parameters-and-meat-quality-in-pigs
#10
Xiang Xu, Lv-Mu Li, Bin Li, Wenjie Guo, Xiaoling Ding, Fazhi Xu
Objective: This study investigated the effect of fermented biogas residue (FBR) of wheat on the performance, serum biochemical parameters, and meat quality in pigs. Methods: We selected 128 pigs (the mean initial body weight is 40.24±3.08 kg) and randomly allocated them to 4 groups (1 control group and 3 treatment groups) with 4 replicates per group and 8 pigs per pen in a randomized complete block design based on initial body weight and sex. The control group received soybean meal, the treatment group fed diets containing 5%, 10%, and 15% fermented biomass residue (FBR), respectively (abbreviated as FBR5, FBR10 and FBR15, respectively)...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28423883/comparison-of-growth-performance-of-berkshire-purebreds-and-crossbreds-sired-by-hereford-and-tamworth-breeds-raised-in-alternative-production-system
#11
Hyeon-Suk Park, Kristal Spann, Niki Whitley, Sang-Hyon Oh
Objective: The objective of the present study was to compare growth performance of Berkshire purebred pigs (BB), Hereford (HB) and/or Tamworth (TB) sired Berkshire crossbred pigs reared in a hoop structure in two experiments. Methods: In the first experiment, BB was compared to TB while HB and TB were compared in the second. Body weights (BW) were recorded at 3 days of age and every 28 days from birth until 140 days of age. There was no significant difference between the BW of BB and TB, but HB was heavier than TB by 84 days of age...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28423882/various-levels-of-rapeseed-meal-in-weaning-pig-diet-from-weaning-to-finishing-periods
#12
Sung Ho Do, Byeong Ock Kim, Lin Hu Fang, Dong Hyeon You, Jin Su Hong, Y Y Kim
Objective: This experiment was conducted to investigate the influence of rapeseed meal (RSM) supplementation in weaning pig diet on growth performance, blood profile, carcass characteristics and economic analysis on weaning to finishing pigs. Methods: A total of 120 cross bred ([Yorkshire × Landrace] × Duroc) weaning pigs were allotted to 5 treatments in a randomized complete block (RCB) design. Each treatment had 4 replications with 6 pigs per pen. Five different levels of RSM (0, 2, 4, 6 and 8%) were used as dietary treatments...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28417300/a-live-gi-ge-deleted-pseudorabies-virus-prv-protects-weaned-piglets-against-lethal-variant-prv-challenge
#13
Yue Yin, Zhiwen Xu, Xiaowan Liu, Ping Li, Fan Yang, Jun Zhao, Yi Fan, Xiangang Sun, Ling Zhu
Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) through homologous DNA recombination. Compared to parental strain, rPRVXJ-delgI/gE-EGFP showed similar growth kinetics in vitro. Its safety and immunogenicity were evaluated in weaned piglets. Our results showed that piglets immunized with rPRVXJ-delgI/gE-EGFP did not exhibit any clinical symptoms, and a high level of gB-specific antibody was detected...
April 17, 2017: Virus Genes
https://www.readbyqxmd.com/read/28412472/preparation-and-characterization-of-dutasteride-loaded-nanostructured-lipid-carriers-coated-with-stearic-acid-chitosan-oligomer-for-topical-delivery
#14
Norhayati Mohamed Noor, Khalid Sheikh, Satyanarayana Somavarapu, Kevin Mg Taylor
Dutasteride, used for treating benign prostate hyperplasia (BPH), promotes hair growth. To enhance delivery to the hair follicles and reduce systemic effects, in this study dutasteride has been formulated for topical application, in a nanostructured lipid carrier (NLC) coated with chitosan oligomer-stearic acid (CSO-SA). CSO-SA has been successfully synthesized, as confirmed using (1)H NMR and FTIR. Formulation of dutasteride-loaded nanostructured lipid carriers (DST-NLCs) was optimised using 2(3) full factorial design...
April 12, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28407326/maternal-lactocrine-programming-of-porcine-reproductive-tract-development
#15
REVIEW
Carol A Bagnell, Teh-Yuan Ho, Ashley F George, Anne A Wiley, Dori J Miller, Frank F Bartol
The lactocrine hypothesis for maternal programming of female reproductive tract development is based on the idea that non-nutritive, milk-borne bioactive factors (MbFs), delivered from mother to offspring during nursing, play a role in determining the trajectory of development with long-term consequences in the adult. Porcine female reproductive tract development is completed postnatally, and the period during which maternal support of neonatal growth derives exclusively from colostrum/milk defines a window of opportunity for lactocrine programming of reproductive tissues...
April 13, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28405457/factors-for-improving-reproductive-performance-of-sows-and-herd-productivity-in-commercial-breeding-herds
#16
REVIEW
Yuzo Koketsu, Satomi Tani, Ryosuke Iida
We review critical factors associated with reproductive performance of female breeding pigs, their lifetime performance and herd productivity in commercial herds. The factors include both sow-level and herd-level factors. High risk sow-level groups for decreasing reproductive performance of female pigs are low or high parity, increased outdoor temperature, decreased lactation feed intake, single inseminations, increased lactation length, prolonged weaning-to-first-mating interval, low birth weight or low preweaning growth rate, a few pigs born alive at parity 1, an increased number of stillborn piglets, foster-in or nurse sow practices and low or high age at first-mating...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28403547/effects-of-dietary-supplementation-with-a-probiotic-enterococcus-faecium-dsm-7134-on-growth-performance-nutrient-digestibility-and-gut-health-status-in-weaning-pigs
#17
R X Lan, I H Kim
BACKGROUND: Probiotics have been suggested as desirable antibiotic alternatives to pigs by improving gut health and growth performance. However, limited studies of the effectiveness of E. faecium in weaning pigs, the objective of this study was to investigate the effects of E. faecium DSM 7134 supplementation on growth performance, nutrient digestibility, antioxidant parameters, volatile fatty acids concentration, intestinal histomorphology, and diarrhea score in weaning pig. RESULTS: E...
April 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28402050/effects-of-dietary-supplementation-with-tribasic-zinc-sulfate-or-zinc-sulfate-on-growth-performance-zinc-content-and-expression-of-zinc-transporters-in-young-pigs
#18
Bo Deng, Xihong Zhou, Jie Wu, Ciming Long, Yajun Yao, Hongxing Peng, Dan Wan, Xin Wu
An experiment was conducted to compare the effects of zinc sulfate (ZS) and tribasic zinc sulfate (TBZ) as sources of supplemental zinc on growth performance, serum zinc (Zn) content and messenger RNA (mRNA) expression of Zn transporters (ZnT1/ZnT2/ZnT5/ZIP4/DMT1) of young growing pigs. A total of 96 Duroc × Landrace × Yorkshire pigs were randomly allotted to two treatments and were fed a basal diet supplemented with 100 mg/kg Zn from either ZS or TBZ for 28 days. Feed : gain ratio in pigs fed TBZ were lower (P < 0...
April 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28400270/differential-expression-profile-of-mirnas-in-porcine-muscle-and-adipose-tissue-during-development
#19
Qi Wang, Renli Qi, Jing Wang, Wenming Huang, Yongjiang Wu, Huang Xiaofeng, Feiyun Yang, Jinxiu Huang
MicroRNAs (miRNAs) are a class of non-coding RNAs that play a crucial regulatory role in many biological processes. Previous studies have reported miRNAs that are associated with the growth, differentiation, and proliferation of myocytes and adipocytes in pigs. However, differences in the miRNA expression profiles between muscle and adipose tissues during porcine development are unknown. Muscle and adipose tissues are the two major organs that are crucial for dynamic energy balance in the development and metabolism...
April 8, 2017: Gene
https://www.readbyqxmd.com/read/28399888/a-guinea-pig-model-of-zika-virus-infection
#20
Mukesh Kumar, Keeton K Krause, Francine Azouz, Eileen Nakano, Vivek R Nerurkar
BACKGROUND: Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemic of Zika virus (ZIKV). Here we report that immunocompetent guinea pigs are susceptible to infection by a contemporary American strain of ZIKV. METHODS: Dunkin-Hartley guinea pigs were inoculated with 10(6) plaque-forming units of ZIKV via subcutaneous route and clinical signs were observed. Viremia, viral load in the tissues, anti-ZIKV neutralizing antibody titer, and protein levels of multiple cytokine and chemokines were analyzed using qRT-PCR, plaque assay, plaque reduction neutralization test (PRNT) and multiplex immunoassay...
April 11, 2017: Virology Journal
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