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Aspergillus fumigatus RNA-seq

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https://www.readbyqxmd.com/read/26273834/transcriptome-profiles-of-human-lung-epithelial-cells-a549-interacting-with-aspergillus-fumigatus-by-rna-seq
#1
Fangyan Chen, Changjian Zhang, Xiaodong Jia, Shuo Wang, Jing Wang, Yong Chen, Jingya Zhao, Shuguang Tian, Xuelin Han, Li Han
Lung epithelial cells constitute the first defense line of host against the inhaled Aspergillus fumigatus; however, the transcriptional response of human alveolar type II epithelial cells was still unclear. Here we used RNA-Seq technology to assess the transcriptome profiles of A549 cells following direct interaction with conidia of A. fumigatus. The total number of identified genes was 19118. Compared with uninfected A549 cells, 459 genes were differentially expressed in cells co-incubated with conidia for 8 h, including 302 up-regulated genes and 157 down-regulated genes...
2015: PloS One
https://www.readbyqxmd.com/read/26100701/transcription-factor-ads-4-regulates-adaptive-responses-and-resistance-to-antifungal-azole-stress
#2
Kangji Wang, Zhenying Zhang, Xi Chen, Xianyun Sun, Cheng Jin, Hongwei Liu, Shaojie Li
Azoles are commonly used as antifungal drugs or pesticides to control fungal infections in medicine and agriculture. Fungi adapt to azole stress by rapidly activating the transcription of a number of genes, and transcriptional increases in some azole-responsive genes can elevate azole resistance. The regulatory mechanisms that control transcriptional responses to azole stress in filamentous fungi are not well understood. This study identified a bZIP transcription factor, ADS-4 (antifungal drug sensitive-4), as a new regulator of adaptive responses and resistance to antifungal azoles...
September 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25776795/the-newly-nonsporulated-characterization-of-an-aspergillus-fumigatus-isolate-from-an-immunocompetent-patient-and-its-clinic-indication
#3
Caiyun Zhang, Qingtao Kong, Zhendong Cai, Fang Liu, Peiying Chen, Jinxing Song, Ling Lu, Hong Sang
Aspergillus fumigatus (A. fumigatus) commonly produces abundant and heavily melanized infectious conidia, which are the primary agents that cause invasive aspergillosis (IA) in immunocompromised patients. We isolated a white nonsporulating A. fumigatus strain (A1j) from an immunocompetent patient. It was identified by histopathological examination and morphological observation, and subsequently confirmed by DNA sequencing of internal transcribed spacer (ITS) regions and partial β-tubulin genes. Neither a long waiting time nor passage on various medium types could stimulate the formation of spores and pigment...
August 2015: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/25311525/rna-seq-reveals-the-pan-transcriptomic-impact-of-attenuating-the-gliotoxin-self-protection-mechanism-in-aspergillus-fumigatus
#4
Grainne O'Keeffe, Stephen Hammel, Rebecca A Owens, Thomas M Keane, David A Fitzpatrick, Gary W Jones, Sean Doyle
BACKGROUND: Aspergillus fumigatus produces a number of secondary metabolites, one of which, gliotoxin, has been shown to exhibit anti-fungal activity. Thus, A. fumigatus must be able to protect itself against gliotoxin. Indeed one of the genes in the gliotoxin biosynthetic gene cluster in A. fumigatus, gliT, is required for self-protection against the toxin- however the global self-protection mechanism deployed is unclear. RNA-seq was employed to identify genes differentially regulated upon exposure to gliotoxin in A...
2014: BMC Genomics
https://www.readbyqxmd.com/read/25111616/serpinb3-b4-contributes-to-early-inflammation-and-barrier-dysfunction-in-an-experimental-murine-model-of-atopic-dermatitis
#5
Umasundari Sivaprasad, Kayla G Kinker, Mark B Ericksen, Mark Lindsey, Aaron M Gibson, Stacey A Bass, Nicolas S Hershey, Jingyuan Deng, Mario Medvedovic, Gurjit K Khurana Hershey
Serine proteases are critical for epidermal barrier homeostasis, and their aberrant expression and/or activity is associated with chronic skin diseases. Elevated levels of the serine protease inhibitors SERPINB3 and SERPINB4 are seen in patients with atopic dermatitis and psoriasis. However, their mechanistic role in the skin is unknown. To evaluate the contribution of Serpinb3a (mouse homolog of SERPINB3 and SERPINB4) in atopic dermatitis, we examined the effect of topical Aspergillus fumigatus extract exposure in wild-type and Serpinb3a-null mice on transepidermal water loss (TEWL), sensitization, and inflammation...
January 2015: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/24194595/the-aspergillus-genome-database-multispecies-curation-and-incorporation-of-rna-seq-data-to-improve-structural-gene-annotations
#6
Gustavo C Cerqueira, Martha B Arnaud, Diane O Inglis, Marek S Skrzypek, Gail Binkley, Matt Simison, Stuart R Miyasato, Jonathan Binkley, Joshua Orvis, Prachi Shah, Farrell Wymore, Gavin Sherlock, Jennifer R Wortman
The Aspergillus Genome Database (AspGD; http://www.aspgd.org) is a freely available web-based resource that was designed for Aspergillus researchers and is also a valuable source of information for the entire fungal research community. In addition to being a repository and central point of access to genome, transcriptome and polymorphism data, AspGD hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available Aspergillus genomes...
January 2014: Nucleic Acids Research
https://www.readbyqxmd.com/read/23230834/the-diverse-applications-of-rna-seq-for-functional-genomic-studies-in-aspergillus-fumigatus
#7
Antonis Rokas, John G Gibbons, Xiaofan Zhou, Anne Beauvais, Jean-Paul Latgé
The deep sequencing of an mRNA population, RNA-seq, is a very successful application of next-generation sequencing technologies (NGSTs). RNA-seq takes advantage of two key NGST features: (1) samples can be mixtures of different DNA pieces, and (2) sequencing provides both qualitative and quantitative information about each DNA piece analyzed. We recently used RNA-seq to study the transcriptome of Aspergillus fumigatus, a deadly human fungal pathogen. Analysis of the RNA-seq data indicates that there are likely tens of unannotated and hundreds of novel genes in the A...
December 2012: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/23031507/comparison-of-transcriptome-technologies-in-the-pathogenic-fungus-aspergillus-fumigatus-reveals-novel-insights-into-the-genome-and-mpka-dependent-gene-expression
#8
COMPARATIVE STUDY
Sebastian Müller, Clara Baldin, Marco Groth, Reinhard Guthke, Olaf Kniemeyer, Axel A Brakhage, Vito Valiante
BACKGROUND: The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immuno-compromised patients. Recently developed high-throughput transcriptome and proteome technologies, such as microarrays, RNA deep-sequencing, and LC-MS/MS of peptide mixtures, are of enormous value for systematically investigating pathogenic organisms. In the field of infection biology, one of the priorities is to collect and standardise data, in order to generate datasets that can be used to investigate and compare pathways and gene responses involved in pathogenicity...
2012: BMC Genomics
https://www.readbyqxmd.com/read/21724936/global-transcriptome-changes-underlying-colony-growth-in-the-opportunistic-human-pathogen-aspergillus-fumigatus
#9
John G Gibbons, Anne Beauvais, Remi Beau, Kriston L McGary, Jean-Paul Latgé, Antonis Rokas
Aspergillus fumigatus is the most common and deadly pulmonary fungal infection worldwide. In the lung, the fungus usually forms a dense colony of filaments embedded in a polymeric extracellular matrix. To identify candidate genes involved in this biofilm (BF) growth, we used RNA-Seq to compare the transcriptomes of BF and liquid plankton (PL) growth. Sequencing and mapping of tens of millions sequence reads against the A. fumigatus transcriptome identified 3,728 differentially regulated genes in the two conditions...
January 2012: Eukaryotic Cell
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