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Christopher contag

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https://www.readbyqxmd.com/read/28631141/cell-labeling-with-magneto-endosymbionts-and-the-dissection-of-the-subcellular-location-fate-and-host-cell-interactions
#1
Kayla R Lee, Abdul Wakeel, Papia Chakraborty, Chandler S Foote, Lauren Kajiura, Joyce C Barrozo, Andrea C Chan, Alexey V Bazarov, Ryan Spitler, Peter M Kutny, Jim M Denegre, Rob A Taft, Joachim Seemann, Bradley W Rice, Christopher H Contag, Brian K Rutt, Caleb B Bell
PURPOSE: The purposes of this study are to characterize magneto-endosymbiont (ME) labeling of mammalian cells and to discern the subcellular fate of these living contrast agents. MEs are novel magnetic resonance imaging (MRI) contrast agents that are being used for cell tracking studies. Understanding the fate of MEs in host cells is valuable for designing in vivo cell tracking experiments. PROCEDURES: The ME's surface epitopes, contrast-producing paramagnetic magnetosomal iron, and genome were studied using immunocytochemistry (ICC), Fe and MRI contrast measurements, and quantitative polymerase chain reaction (qPCR), respectively...
June 19, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28616842/characterization-of-magneto-endosymbionts-as-mri-cell-labeling-and-tracking-agents
#2
Kimberly D Brewer, Ryan Spitler, Kayla R Lee, Andrea C Chan, Joyce C Barrozo, Abdul Wakeel, Chandler S Foote, Steven Machtaler, James Rioux, Juergen K Willmann, Papia Chakraborty, Bradley W Rice, Christopher H Contag, Caleb B Bell, Brian K Rutt
PURPOSE: Magneto-endosymbionts (MEs) show promise as living magnetic resonance imaging (MRI) contrast agents for in vivo cell tracking. Here we characterize the biomedical imaging properties of ME contrast agents, in vitro and in vivo. PROCEDURES: By adapting and engineering magnetotactic bacteria to the intracellular niche, we are creating magneto-endosymbionts (MEs) that offer advantages relative to passive iron-based contrast agents (superparamagnetic iron oxides, SPIOs) for cell tracking...
June 14, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28591078/proximal-junctional-kyphosis-inter-and-intra-observer-reliability-of-radiographic-measurements-in-adult-spinal-deformity
#3
Farbod Rastegar, Alec Contag, Alan Daniels, Jayme Hiratzka, Clifford Lin, Jason Chang, Khoi Than, Ahmed Raslan, Christopher Kong, Ngoc-Lam Nguyen, Richard Hostin, Marie Kane, Robert Hart
STUDY DESIGN: Reliability study of radiographic measures of proximal junctional kyphosis in adult spinal deformity patients. OBJECTIVE: Assess impacts of level of proximal endpoint and vertebral fracture on reliability of measurement of junctional kyphosis. SUMMARY OF BACKGROUND DATA: Radiographic assessment is important in determining management of patients with Proximal Junctional Kyphosis (PJK) or Proximal Junctional Failure (PJF). No study to date has evaluated the reliability of radiographic measurement of the junctional kyphotic angle after surgery for Adult Spinal Deformity (ASD)...
June 6, 2017: Spine
https://www.readbyqxmd.com/read/28490473/specific-imaging-of-bacterial-infection-using-6-18-f-fluoromaltotriose-a-second-generation-pet-tracer-targeting-the-maltodextrin-transporter-in-bacteria
#4
Gayatri Gowrishankar, Jonathan Hardy, Mirwais Wardak, Mohammad Namavari, Robert Reeves, Evgenios Neofytou, Ananth Srinivasan, Joseph Wu, Christopher Contag, Sanjiv Gambhir
Purpose: 6"-(18)F-fluoromaltotriose is a novel positron emission tomography (PET) tracer that can potentially be used to image and localize most bacterial infections, much like 2-deoxy-2-(18)F-fluoro-D-glucose ((18)F-FDG) has been used to image and localize many cancers. However, unlike (18)F-FDG, 6"-(18)F-fluoromaltotriose is not taken up by inflammatory lesions and appears to be specific to bacterial infections by targeting the maltodextrin transporter that is expressed in most Gram-positive and Gram-negative strains of bacteria...
May 10, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28428283/regulatory-aspects-of-optical-methods-and-exogenous-targets-for-cancer-detection
#5
Willemieke S Tummers, Jason M Warram, Kiranya E Tipirneni, John Fengler, Paula Jacobs, Lalitha Shankar, Lori Henderson, Betsy Ballard, Brian W Pogue, Jamey P Weichert, Michael Bouvet, Jonathan Sorger, Christopher H Contag, John V Frangioni, Michael F Tweedle, James P Basilion, Sanjiv S Gambhir, Eben L Rosenthal
Considerable advances in cancer-specific optical imaging have improved the precision of tumor resection. In comparison to traditional imaging modalities, this technology is unique in its ability to provide real-time feedback to the operating surgeon. Given the significant clinical implications of optical imaging, there is an urgent need to standardize surgical navigation tools and contrast agents to facilitate swift regulatory approval. Because fluorescence-enhanced surgery requires a combination of both device and drug, each may be developed in conjunction, or separately, which are important considerations in the approval process...
April 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/28385925/neutrophil-myeloperoxidase-diminishes-the-toxic-effects-and-mortality-induced-by-lipopolysaccharide
#6
Laurent L Reber, Caitlin M Gillis, Philipp Starkl, Friederike Jönsson, Riccardo Sibilano, Thomas Marichal, Nicolas Gaudenzio, Marion Bérard, Stephan Rogalla, Christopher H Contag, Pierre Bruhns, Stephen J Galli
Neutrophils have crucial antimicrobial functions but are also thought to contribute to tissue injury upon exposure to bacterial products, such as lipopolysaccharide (LPS). To study the role of neutrophils in LPS-induced endotoxemia, we developed a new mouse model, PMN(DTR) mice, in which injection of diphtheria toxin induces selective neutrophil ablation. Using this model, we found, surprisingly, that neutrophils serve to protect the host from LPS-induced lethal inflammation. This protective role was observed in conventional and germ-free animal facilities, indicating that it does not depend on a particular microbiological environment...
May 1, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28224324/targeted-nanomeetings-within-a-larger-delivery-congress
#7
EDITORIAL
Christopher H Contag
No abstract text is available yet for this article.
February 21, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28069945/charge-altering-releasable-transporters-carts-for-the-delivery-and-release-of-mrna-in-living-animals
#8
Colin J McKinlay, Jessica R Vargas, Timothy R Blake, Jonathan W Hardy, Masamitsu Kanada, Christopher H Contag, Paul A Wender, Robert M Waymouth
Functional delivery of mRNA to tissues in the body is key to implementing fundamentally new and potentially transformative strategies for vaccination, protein replacement therapy, and genome editing, collectively affecting approaches for the prevention, detection, and treatment of disease. Broadly applicable tools for the efficient delivery of mRNA into cultured cells would advance many areas of research, and effective and safe in vivo mRNA delivery could fundamentally transform clinical practice. Here we report the step-economical synthesis and evaluation of a tunable and effective class of synthetic biodegradable materials: charge-altering releasable transporters (CARTs) for mRNA delivery into cells...
January 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27748701/patient-satisfaction-after-adult-spinal-deformity-surgery-does-not-strongly-correlate-with-health-related-quality-of-life-scores-radiographic-parameters-or-occurrence-of-complications
#9
David Kojo Hamilton, Christopher Kong, Jayme Hiratzka, Alec G Contag, Tamir Ailon, Breton Line, Alan Daniels, Justin S Smith, Peter Passias, Themistocles Protopsaltis, Daniel Sciubba, Douglas Burton, Christopher Shaffrey, Eric Klineberg, Gregory Mundis, Han-Jo Kim, Virginie Lafage, Renaud Lafage, Justin Scheer, Oheneba Boachie-Adjei, Shay Bess, Robert A Hart
STUDY DESIGN: This is a multicenter retrospective review of prospectively collected cases. OBJECTIVE: Our objective was to evaluate the relationship between patient satisfaction, health-related quality of life (HRQoL) scores, complications, and radiographic measures at 2 years postoperative follow-up. SUMMARY OF BACKGROUND DATA: For patients receiving operative management for adult spine deformity (ASD), the relationship between HRQoL measures, radiographic parameters, postoperative complications, and self-reported satisfaction remains unclear...
May 15, 2017: Spine
https://www.readbyqxmd.com/read/27679430/selecting-caudal-fusion-levels-2%C3%A2-year-functional-and-stiffness-outcomes-with-matched-pairs-analysis-in-multilevel-fusion-to-l5-versus-s1
#10
Alan H Daniels, Heiko Koller, Shannon L Hiratzka, Michael Mayer, Oliver Meier, Alec Gabriel Contag, Adam E M Eltorai, Jayme Hiratzka, D Kojo Hamilton, Christopher I Shaffrey, Justin S Smith, Shay Bess, Eric O Klineberg, Christopher P Ames, Virginie Lafage, Breton Line, Frank J Schwab, Robert A Hart, International Spine Study Group
PURPOSE: Controversy persists as to whether to end multilevel thoracolumbar fusions caudally at L5 or S1. Some argue that stopping at L5 may preserve greater function, but there are few data comparing functional limitations due to lumbar stiffness in patients with fusion to L5 versus S1. The aim of this study was to evaluate whether patients undergoing multilevel thoracolumbar fusions with an L5 caudal endpoint have a better lumbosacral function than patients with an S1 caudal endpoint...
September 27, 2016: European Spine Journal
https://www.readbyqxmd.com/read/27154508/a-clinical-wide-field-fluorescence-endoscopic-device-for-molecular-imaging-demonstrating-cathepsin-protease-activity-in-colon-cancer
#11
Steven Sensarn, Cristina L Zavaleta, Ehud Segal, Stephan Rogalla, Wansik Lee, Sanjiv S Gambhir, Matthew Bogyo, Christopher H Contag
PURPOSE: Early and effective detection of cancers of the gastrointestinal tract will require novel molecular probes and advances in instrumentation that can reveal functional changes in dysplastic and malignant tissues. Here, we describe adaptation of a wide-field clinical fiberscope to perform wide-field fluorescence imaging while preserving its white-light capability for the purpose of providing wide-field fluorescence imaging capability to point-of-care microscopes. PROCEDURES: We developed and used a fluorescent fiberscope to detect signals from a quenched probe, BMV109, that becomes fluorescent when cleaved by, and covalently bound to, active cathepsin proteases...
December 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/27080321/world-molecular-imaging-congress-2016-imaging-biology-improving-therapy
#12
EDITORIAL
Fabian Kiessling, Christopher H Contag
No abstract text is available yet for this article.
June 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/27026807/a-role-of-the-adaptive-immune-system-in-glucose-homeostasis
#13
Laura L Bronsart, Christopher H Contag
OBJECTIVE: The immune system, including the adaptive immune response, has recently been recognized as having a significant role in diet-induced insulin resistance. In this study, we aimed to determine if the adaptive immune system also functions in maintaining physiological glucose homeostasis in the absence of diet-induced disease. RESEARCH DESIGN AND METHODS: SCID mice and immunocompetent control animals were phenotypically assessed for variations in metabolic parameters and cytokine profiles...
2016: BMJ Open Diabetes Research & Care
https://www.readbyqxmd.com/read/26883480/more-chemistry-is-needed-for-molecular-imaging
#14
EDITORIAL
Jianghong Rao, Christopher H Contag
No abstract text is available yet for this article.
February 17, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/26873653/reactive-oxygen-species-imaging-in-a-mouse-model-of-inflammatory-bowel-disease
#15
Laura Bronsart, Linh Nguyen, Aida Habtezion, Christopher Contag
PURPOSE: Reactive oxygen species (ROS) are important contributors to inflammatory bowel disease (IBD); however, there are insufficient tools for their in vivo evaluation. PROCEDURES: To determine if a chemiluminescent ROS reporter, coelenterazine, would be a useful tool for the detection of immune cell activation, the macrophage cell line (RAW 264.7) was treated with phorbol myristate acetate (PMA). Additionally, coelenterazine was used to monitor the changes in ROS production over time in a mouse model of IBD...
August 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/26530911/imaging-functional-nucleic-acid-delivery-to-skin
#16
Roger L Kaspar, Robyn P Hickerson, Emilio González-González, Manuel A Flores, Tycho P Speaker, Faye A Rogers, Leonard M Milstone, Christopher H Contag
Monogenic skin diseases arise from well-defined single gene mutations, and in some cases a single point mutation. As the target cells are superficial, these diseases are ideally suited for treatment by nucleic acid-based therapies as well as monitoring through a variety of noninvasive imaging technologies. Despite the accessibility of the skin, there remain formidable barriers for functional delivery of nucleic acids to the target cells within the dermis and epidermis. These barriers include the stratum corneum and the layered structure of the skin, as well as more locally, the cellular, endosomal and nuclear membranes...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26169581/non-invasive-intravital-imaging-of-sirna-mediated-mutant-keratin-gene-repression-in-skin
#17
Robyn P Hickerson, Tycho J Speaker, Maria Fernanda Lara, Emilio González-González, Manuel A Flores, Christopher H Contag, Roger L Kaspar
PURPOSE: Small interfering RNAs (siRNAs) specifically and potently inhibit target gene expression. Pachyonychia congenita (PC) is a skin disorder caused by mutations in genes encoding keratin (K) 6a/b, K16, and K17, resulting in faulty intermediate filaments. A siRNA targeting a single nucleotide, PC-relevant mutation inhibits K6a expression and has been evaluated in the clinic with encouraging results. PROCEDURES: To better understand the pathophysiology of PC, and develop a model system to study siRNA delivery and visualize efficacy in skin, wild type (WT) and mutant K6a complementary DNAs (cDNAs) were fused to either enhanced green fluorescent protein or tandem tomato fluorescent protein cDNA to allow covisualization of mutant and WT K6a expression in mouse footpad skin using a dual fluorescence in vivo confocal imaging system equipped with 488 and 532 nm lasers...
February 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/26049697/early-cancer-detection-at-the-epithelial-surface
#18
REVIEW
Stephan Rogalla, Christopher H Contag
Malignant neoplastic lesions derived from epithelial tissue, carcinomas, account for 80% to 100% of all human cancers including some of the most deadly diseases such as cervical and non-small cell lung cancer. Many of these carcinomas present at readily accessible epithelial surfaces offering unique detection opportunities. Effective clinical management of carcinomas is enabled by early detection, at a time when full surgical resection is possible and before invasion of adjacent tissue or significant intravasation into blood vessels leading to metastasis...
May 2015: Cancer Journal
https://www.readbyqxmd.com/read/25923788/a-real-time-clinical-endoscopic-system-for-intraluminal-multiplexed-imaging-of-surface-enhanced-raman-scattering-nanoparticles
#19
Ellis Garai, Steven Sensarn, Cristina L Zavaleta, Nathan O Loewke, Stephan Rogalla, Michael J Mandella, Stephen A Felt, Shai Friedland, Jonathan T C Liu, Sanjiv S Gambhir, Christopher H Contag
The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e...
2015: PloS One
https://www.readbyqxmd.com/read/25867136/methods-for-culturing-human-femur-tissue-explants-to-study-breast-cancer-cell-colonization-of-the-metastatic-niche
#20
Zachary S Templeton, Michael H Bachmann, Rajiv V Alluri, William J Maloney, Christopher H Contag, Bonnie L King
Bone is the most common site of breast cancer metastasis. Although it is widely accepted that the microenvironment influences cancer cell behavior, little is known about breast cancer cell properties and behaviors within the native microenvironment of human bone tissue.We have developed approaches to track, quantify and modulate human breast cancer cells within the microenvironment of cultured human bone tissue fragments isolated from discarded femoral heads following total hip replacement surgeries. Using breast cancer cells engineered for luciferase and enhanced green fluorescent protein (EGFP) expression, we are able to reproducibly quantitate migration and proliferation patterns using bioluminescence imaging (BLI), track cell interactions within the bone fragments using fluorescence microscopy, and evaluate breast cells after colonization with flow cytometry...
2015: Journal of Visualized Experiments: JoVE
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