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Christopher contag

D Kojo Hamilton, Christopher Kong, Jayme Hiratzka, Alec G Contag, Tamir Ailon, Breton Line, Alan Daniels, Justin S Smith, Peter Passias, Themistocles Protopsaltis, Daniel Sciubba, Douglas Burton, Christopher Shaffrey, Eric Klineberg, Gregory Mundis, Han-Jo Kim, Virginie Lafage, Renaud Lafage, Justin Scheer, Oheneba Boachie-Adjei, Shay Bess, Robert Hart
STUDY DESIGN: This is a multicenter retrospective review of prospectively collected cases. OBJECTIVE: Our objective was to evaluate the relationship between patient satisfaction, HRQoL scores, complications, and radiographic measures at 2 years post-operative follow-up. SUMMARY OF BACKGROUND DATA: For patients receiving operative management for adult spine deformity (ASD), the relationship between health-related quality of life (HRQoL) measures, radiographic parameters, post-operative complications, and self-reported satisfaction remains unclear...
September 29, 2016: Spine
Alan H Daniels, Heiko Koller, Shannon L Hiratzka, Michael Mayer, Oliver Meier, Alec Gabriel Contag, Adam E M Eltorai, Jayme Hiratzka, D Kojo Hamilton, Christopher I Shaffrey, Justin S Smith, Shay Bess, Eric O Klineberg, Christopher P Ames, Virginie Lafage, Breton Line, Frank J Schwab, Robert A Hart, International Spine Study Group
PURPOSE: Controversy persists as to whether to end multilevel thoracolumbar fusions caudally at L5 or S1. Some argue that stopping at L5 may preserve greater function, but there are few data comparing functional limitations due to lumbar stiffness in patients with fusion to L5 versus S1. The aim of this study was to evaluate whether patients undergoing multilevel thoracolumbar fusions with an L5 caudal endpoint have a better lumbosacral function than patients with an S1 caudal endpoint...
September 27, 2016: European Spine Journal
Steven Sensarn, Cristina L Zavaleta, Ehud Segal, Stephan Rogalla, Wansik Lee, Sanjiv S Gambhir, Matthew Bogyo, Christopher H Contag
PURPOSE: Early and effective detection of cancers of the gastrointestinal tract will require novel molecular probes and advances in instrumentation that can reveal functional changes in dysplastic and malignant tissues. Here, we describe adaptation of a wide-field clinical fiberscope to perform wide-field fluorescence imaging while preserving its white-light capability for the purpose of providing wide-field fluorescence imaging capability to point-of-care microscopes. PROCEDURES: We developed and used a fluorescent fiberscope to detect signals from a quenched probe, BMV109, that becomes fluorescent when cleaved by, and covalently bound to, active cathepsin proteases...
May 6, 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Fabian Kiessling, Christopher H Contag
No abstract text is available yet for this article.
June 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Laura L Bronsart, Christopher H Contag
OBJECTIVE: The immune system, including the adaptive immune response, has recently been recognized as having a significant role in diet-induced insulin resistance. In this study, we aimed to determine if the adaptive immune system also functions in maintaining physiological glucose homeostasis in the absence of diet-induced disease. RESEARCH DESIGN AND METHODS: SCID mice and immunocompetent control animals were phenotypically assessed for variations in metabolic parameters and cytokine profiles...
2016: BMJ Open Diabetes Research & Care
Jianghong Rao, Christopher H Contag
No abstract text is available yet for this article.
February 17, 2016: Bioconjugate Chemistry
Laura Bronsart, Linh Nguyen, Aida Habtezion, Christopher Contag
PURPOSE: Reactive oxygen species (ROS) are important contributors to inflammatory bowel disease (IBD); however, there are insufficient tools for their in vivo evaluation. PROCEDURES: To determine if a chemiluminescent ROS reporter, coelenterazine, would be a useful tool for the detection of immune cell activation, the macrophage cell line (RAW 264.7) was treated with phorbol myristate acetate (PMA). Additionally, coelenterazine was used to monitor the changes in ROS production over time in a mouse model of IBD...
August 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Roger L Kaspar, Robyn P Hickerson, Emilio González-González, Manuel A Flores, Tycho P Speaker, Faye A Rogers, Leonard M Milstone, Christopher H Contag
Monogenic skin diseases arise from well-defined single gene mutations, and in some cases a single point mutation. As the target cells are superficial, these diseases are ideally suited for treatment by nucleic acid-based therapies as well as monitoring through a variety of noninvasive imaging technologies. Despite the accessibility of the skin, there remain formidable barriers for functional delivery of nucleic acids to the target cells within the dermis and epidermis. These barriers include the stratum corneum and the layered structure of the skin, as well as more locally, the cellular, endosomal and nuclear membranes...
2016: Methods in Molecular Biology
Robyn P Hickerson, Tycho J Speaker, Maria Fernanda Lara, Emilio González-González, Manuel A Flores, Christopher H Contag, Roger L Kaspar
PURPOSE: Small interfering RNAs (siRNAs) specifically and potently inhibit target gene expression. Pachyonychia congenita (PC) is a skin disorder caused by mutations in genes encoding keratin (K) 6a/b, K16, and K17, resulting in faulty intermediate filaments. A siRNA targeting a single nucleotide, PC-relevant mutation inhibits K6a expression and has been evaluated in the clinic with encouraging results. PROCEDURES: To better understand the pathophysiology of PC, and develop a model system to study siRNA delivery and visualize efficacy in skin, wild type (WT) and mutant K6a complementary DNAs (cDNAs) were fused to either enhanced green fluorescent protein or tandem tomato fluorescent protein cDNA to allow covisualization of mutant and WT K6a expression in mouse footpad skin using a dual fluorescence in vivo confocal imaging system equipped with 488 and 532 nm lasers...
February 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Stephan Rogalla, Christopher H Contag
Malignant neoplastic lesions derived from epithelial tissue, carcinomas, account for 80% to 100% of all human cancers including some of the most deadly diseases such as cervical and non-small cell lung cancer. Many of these carcinomas present at readily accessible epithelial surfaces offering unique detection opportunities. Effective clinical management of carcinomas is enabled by early detection, at a time when full surgical resection is possible and before invasion of adjacent tissue or significant intravasation into blood vessels leading to metastasis...
May 2015: Cancer Journal
Ellis Garai, Steven Sensarn, Cristina L Zavaleta, Nathan O Loewke, Stephan Rogalla, Michael J Mandella, Stephen A Felt, Shai Friedland, Jonathan T C Liu, Sanjiv S Gambhir, Christopher H Contag
The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e...
2015: PloS One
Zachary S Templeton, Michael H Bachmann, Rajiv V Alluri, William J Maloney, Christopher H Contag, Bonnie L King
Bone is the most common site of breast cancer metastasis. Although it is widely accepted that the microenvironment influences cancer cell behavior, little is known about breast cancer cell properties and behaviors within the native microenvironment of human bone tissue.We have developed approaches to track, quantify and modulate human breast cancer cells within the microenvironment of cultured human bone tissue fragments isolated from discarded femoral heads following total hip replacement surgeries. Using breast cancer cells engineered for luciferase and enhanced green fluorescent protein (EGFP) expression, we are able to reproducibly quantitate migration and proliferation patterns using bioluminescence imaging (BLI), track cell interactions within the bone fragments using fluorescence microscopy, and evaluate breast cells after colonization with flow cytometry...
2015: Journal of Visualized Experiments: JoVE
Hyejun Ra, Emilio González-González, Md Jashim Uddin, Bonnie L King, Alex Lee, Irfan Ali-Khan, Lawrence J Marnett, Jean Y Tang, Christopher H Contag
Non-melanoma skin cancer (NMSC) is the most common form of cancer in the US and its incidence is increasing. The current standard of care is visual inspection by physicians and/or dermatologists, followed by skin biopsy and pathologic confirmation. We have investigated the use of in vivo fluorescence imaging using fluorocoxib A as a molecular probe for early detection and assessment of skin tumors in mouse models of NMSC. Fluorocoxib A targets the cyclooxygenase-2 (COX-2) enzyme that is preferentially expressed by inflamed and tumor tissue, and therefore has potential to be an effective broadly active molecular biomarker for cancer detection...
February 2015: Neoplasia: An International Journal for Oncology Research
Masamitsu Kanada, Michael H Bachmann, Jonathan W Hardy, Daniel Omar Frimannson, Laura Bronsart, Andrew Wang, Matthew D Sylvester, Tobi L Schmidt, Roger L Kaspar, Manish J Butte, A C Matin, Christopher H Contag
Extracellular vesicles (EVs), specifically exosomes and microvesicles (MVs), are presumed to play key roles in cell-cell communication via transfer of biomolecules between cells. The biogenesis of these two types of EVs differs as they originate from either the endosomal (exosomes) or plasma (MVs) membranes. To elucidate the primary means through which EVs mediate intercellular communication, we characterized their ability to encapsulate and deliver different types of macromolecules from transiently transfected cells...
March 24, 2015: Proceedings of the National Academy of Sciences of the United States of America
(no author information available yet)
No abstract text is available yet for this article.
February 2015: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Mark E Lobatto, Claudia Calcagno, Antoine Millon, Max L Senders, Francois Fay, Philip M Robson, Sarayu Ramachandran, Tina Binderup, Maarten P M Paridaans, Steven Sensarn, Stephan Rogalla, Ronald E Gordon, Luis Cardoso, Gert Storm, Josbert M Metselaar, Christopher H Contag, Erik S G Stroes, Zahi A Fayad, Willem J M Mulder
Atherosclerosis is a major cause of global morbidity and mortality that could benefit from novel targeted therapeutics. Recent studies have shown efficient and local drug delivery with nanoparticles, although the nanoparticle targeting mechanism for atherosclerosis has not yet been fully elucidated. Here we used in vivo and ex vivo multimodal imaging to examine permeability of the vessel wall and atherosclerotic plaque accumulation of fluorescently labeled liposomal nanoparticles in a rabbit model. We found a strong correlation between permeability as established by in vivo dynamic contrast enhanced magnetic resonance imaging and nanoparticle plaque accumulation with subsequent nanoparticle distribution throughout the vessel wall...
February 24, 2015: ACS Nano
Jonathan W Hardy, Zoia Levashova, Tobi L Schmidt, Christopher H Contag, Francis G Blankenberg
PURPOSE: Here, we evaluate [(99m)Tc]annexin V-128, an in vivo marker of apoptosis, for single photon emission computed tomography (SPECT) imaging of localization and antibiotic treatment of disseminated bacterial infection, using a well-described mouse model that employs bioluminescent Listeria monocytogenes. PROCEDURES: Sixteen groups of five mice in six separate experiments were infected with bioluminescent Listeria, and in vivo bioluminescence imaging (BLI) was performed each day, to assess the location and extent of infection and response to antibiotics...
June 2015: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Tobi L Schmidt, Robert S Negrin, Christopher H Contag
The promise of cell-based immunotherapies for the treatment of cancer offers the potential of therapeutic synergy with chemo- and radiotherapies that may overcome current limitations leading to durable responses and prevention of recurrence. There is a wide array of cell-based immunotherapies that are either poised to enter cancer clinical trials or are in clinical trials, and many are showing some success. Yet within this field, there are clear obstacles that need to be overcome, including limited access across tissue barriers, development of antigen tolerance, and the immunosuppressive microenvironment of tumors...
May 2014: Immunologic Research
Jun Chu, Russell D Haynes, Stéphane Y Corbel, Pengpeng Li, Emilio González-González, John S Burg, Niloufar J Ataie, Amy J Lam, Paula J Cranfill, Michelle A Baird, Michael W Davidson, Ho-Leung Ng, K Christopher Garcia, Christopher H Contag, Kang Shen, Helen M Blau, Michael Z Lin
A method for non-invasive visualization of genetically labeled cells in animal disease models with micrometer-level resolution would greatly facilitate development of cell-based therapies. Imaging of fluorescent proteins (FPs) using red excitation light in the 'optical window' above 600 nm is one potential method for visualizing implanted cells. However, previous efforts to engineer FPs with peak excitation beyond 600 nm have resulted in undesirable reductions in brightness. Here we report three new red-excitable monomeric FPs obtained by structure-guided mutagenesis of mNeptune...
May 2014: Nature Methods
Sophie Kusy, Christopher H Contag
Advances in noninvasive imaging technologies that allow for in vivo dynamic monitoring of cells and cellular function in living research subjects have revealed new insights into cell biology in the context of intact organs and their native environment. In the field of hematopoiesis and stem cell research, studies of cell trafficking involved in injury repair and hematopoietic engraftment have made great progress using these new tools. Stem cells present unique challenges for imaging since after transplantation, they proliferate dramatically and differentiate...
2014: Methods in Molecular Biology
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