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annelise Barron

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https://www.readbyqxmd.com/read/26849681/in-vivo-in-vitro-and-in-silico-characterization-of-peptoids-as-antimicrobial-agents
#1
Ann M Czyzewski, Håvard Jenssen, Christopher D Fjell, Matt Waldbrook, Nathaniel P Chongsiriwatana, Eddie Yuen, Robert E W Hancock, Annelise E Barron
Bacterial resistance to conventional antibiotics is a global threat that has spurred the development of antimicrobial peptides (AMPs) and their mimetics as novel anti-infective agents. While the bioavailability of AMPs is often reduced due to protease activity, the non-natural structure of AMP mimetics renders them robust to proteolytic degradation, thus offering a distinct advantage for their clinical application. We explore the therapeutic potential of N-substituted glycines, or peptoids, as AMP mimics using a multi-faceted approach that includes in silico, in vitro, and in vivo techniques...
2016: PloS One
https://www.readbyqxmd.com/read/26022845/prostate-tumor-specific-peptide-peptoid-hybrid-prodrugs
#2
Jiyoun Lee, Wei Huang, James M Broering, Annelise E Barron, Jiwon Seo
Inspired by naturally occurring host defense peptides, cationic amphipathic peptoids provide a promising scaffold for anti-cancer therapeutics. Herein, we report a library of peptide-peptoid hybrid prodrugs that can be selectively activated by prostate cancer cells. We have identified several compounds demonstrating potent anti-cancer activity with good to moderate selectivity. We believe that these prodrugs can provide a useful design principle for next generation peptide-peptoid hybrid prodrugs.
July 15, 2015: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/25686659/human-antimicrobial-peptide-ll-37-induces-glial-mediated-neuroinflammation
#3
Moonhee Lee, Xiaolei Shi, Annelise E Barron, Edith McGeer, Patrick L McGeer
LL-37 is the sole cathelicidin-derived antimicrobial peptide found in humans. It becomes active upon C-terminal cleavage of its inactive precursor hCAP18. In addition to antimicrobial action, it also functions as an innate immune system stimulant in many tissues of the body. Here we report that hCAP18 and LL-37 are expressed in all organs of the human body that were studied with the highest basic levels being expressed in the GI tract and the brain. Its expression and functional role in the central nerve system (CNS) has not previously been reported...
March 15, 2015: Biochemical Pharmacology
https://www.readbyqxmd.com/read/24587350/learning-from-host-defense-peptides-cationic-amphipathic-peptoids-with-potent-anticancer-activity
#4
Wei Huang, Jiwon Seo, Stephen B Willingham, Ann M Czyzewski, Mark L Gonzalgo, Irving L Weissman, Annelise E Barron
Cationic, amphipathic host defense peptides represent a promising group of agents to be developed for anticancer applications. Poly-N-substituted glycines, or peptoids, are a class of biostable, peptidomimetic scaffold that can display a great diversity of side chains in highly tunable sequences via facile solid-phase synthesis. Herein, we present a library of anti-proliferative peptoids that mimics the cationic, amphipathic structural feature of the host defense peptides and explore the relationships between the structure, anticancer activity and selectivity of these peptoids...
2014: PloS One
https://www.readbyqxmd.com/read/24484675/a-tunable-silk-alginate-hydrogel-scaffold-for-stem-cell-culture-and-transplantation
#5
Keren Ziv, Harald Nuhn, Yael Ben-Haim, Laura S Sasportas, Paul J Kempen, Thomas P Niedringhaus, Michael Hrynyk, Robert Sinclair, Annelise E Barron, Sanjiv S Gambhir
One of the major challenges in regenerative medicine is the ability to recreate the stem cell niche, which is defined by its signaling molecules, the creation of cytokine gradients, and the modulation of matrix stiffness. A wide range of scaffolds has been developed in order to recapitulate the stem cell niche, among them hydrogels. This paper reports the development of a new silk-alginate based hydrogel with a focus on stem cell culture. This biocomposite allows to fine tune its elasticity during cell culture, addressing the importance of mechanotransduction during stem cell differentiation...
April 2014: Biomaterials
https://www.readbyqxmd.com/read/23694784/the-incorporation-of-extracellular-matrix-proteins-in-protein-polymer-hydrogels-to-improve-encapsulated-beta-cell-function
#6
Liese N Beenken-Rothkopf, Lindsay S Karfeld-Sulzer, Nicolynn E Davis, Ryan Forster, Annelise E Barron, Magali J Fontaine
Biomaterial encapsulation of islets has been proposed to improve the long-term success of islet transplantation by recreating a suitable microenvironment and enhancing cell-matrix interactions that affect cellular function. Protein polymer hydrogels previously showed promise as a biocompatible scaffold by maintaining high cell viability. Here, enzymatically-crosslinked protein polymers were used to investigate the effects of varying scaffold properties and of introducing ECM proteins on the viability and function of encapsulated MIN6 β-cells...
2013: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/23555603/a-readily-applicable-strategy-to-convert-peptides-to-peptoid-based-therapeutics
#7
Minyoung Park, Modi Wetzler, Theodore S Jardetzky, Annelise E Barron
Incorporation of unnatural amino acids and peptidomimetic residues into therapeutic peptides is highly efficacious and commonly employed, but generally requires laborious trial-and-error approaches. Previously, we demonstrated that C20 peptide has the potential to be a potential antiviral agent. Herein we report our attempt to improve the biological properties of this peptide by introducing peptidomimetics. Through combined alanine, proline, and sarcosine scans coupled with a competitive fluorescence polarization assay developed for identifying antiviral peptides, we enabled to pinpoint peptoid-tolerant peptide residues within C20 peptide...
2013: PloS One
https://www.readbyqxmd.com/read/23192597/simultaneous-detection-of-19-k-ras-mutations-by-free-solution-conjugate-electrophoresis-of-ligase-detection-reaction-products-on-glass-microchips
#8
Jennifer Coyne Albrecht, Akira Kotani, Jennifer S Lin, Steven A Soper, Annelise E Barron
We demonstrate here the power and flexibility of free-solution conjugate electrophoresis (FSCE) as a method of separating DNA fragments by electrophoresis with no sieving polymer network. Previous work introduced the coupling of FSCE with ligase detection reaction (LDR) to detect point mutations, even at low abundance compared to the wild-type DNA. Here, four large drag-tags are used to achieve free-solution electrophoretic separation of 19 LDR products ranging in size from 42 to 66 nt that correspond to mutations in the K-ras oncogene...
February 2013: Electrophoresis
https://www.readbyqxmd.com/read/22965707/microfabricated-devices-for-biomolecule-encapsulation
#9
REVIEW
Samantha M Desmarais, Henk P Haagsman, Annelise E Barron
Biomolecule encapsulation in droplets is important for miniaturizing biological assays to reduce reagent consumption, cost and time of analysis, and can be most effectively achieved by using microfabricated devices. Microfabricated fluidic devices can generate emulsified drops of uniform size with controlled dimensions and contents. Biological and chemical components such as cells, microgels, beads, hydrogel precursors, polymer initiators, and other droplets can be encapsulated within these drops. Encapsulated emulsions are appealing for a variety of applications since drops can be used as tiny reaction vessels to perform high-throughput reactions at fast rates, consuming minimal sample and solvent amounts due to the small size (micron diameters) of the emulsion drops...
September 2012: Electrophoresis
https://www.readbyqxmd.com/read/22832218/protein-polymer-hydrogels-effects-of-endotoxin-on-biocompatibility
#10
Liese N Beenken-Rothkopf, Lindsay S Karfeld-Sulzer, Xiaomin Zhang, Hermann Kissler, Sara A Michie, Dixon B Kaufman, Magali J Fontaine, Annelise E Barron
Protein polymer-based hydrogels have shown potential for tissue engineering applications, but require biocompatibility testing for in vivo use. Enzymatically crosslinked protein polymer-based hydrogels were tested in vitro and in vivo to evaluate their biocompatibility. Endotoxins present in the hydrogel were removed by Trition X-114 phase separation. The reduction of endotoxins decreased TNF-α production by a macrophage cell line in vitro; however, significant inflammatory response was still present compared to collagen control gels...
September 2013: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/22828784/peptoid-transporters-effects-of-cationic-amphipathic-structure-on-their-cellular-uptake
#11
Wei Huang, Jiwon Seo, Jennifer S Lin, Annelise E Barron
Two cationic, amphipathic peptoids (poly-N-substituted glycines) were developed as new molecular transporters, which have extensive cellullar uptake and utilize different internalization mechanisms from purely cationic polyguanidine comparators.
October 2012: Molecular BioSystems
https://www.readbyqxmd.com/read/22767501/encapsulation-of-protein-microfiber-networks-supporting-pancreatic-islets
#12
Joseph A M Steele, Annelise E Barron, Euridice Carmona, Jean-Pierre Hallé, Ronald J Neufeld
Networks of discrete, genipin-crosslinked gelatin microfibers enveloping pancreatic islets were incorporated within barium alginate microcapsules. This novel technique enabled encapsulation of cellular aggregates in a spherical fibrous matrix <300 μm in diameter. Microfibers were produced by vortex-drawn extrusion within an alginate support matrix. Optimization culminated in a hydrated fiber diameter of 22.3 ± 0.4 μm, a significant reduction relative to that available through current gelatin microfiber spinning techniques, while making the process more reliable and less labor intensive...
December 2012: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/22766242/enhanced-function-of-pancreatic-islets-co-encapsulated-with-ecm-proteins-and-mesenchymal-stromal-cells-in-a-silk-hydrogel
#13
Nicolynn E Davis, Liese N Beenken-Rothkopf, Annie Mirsoian, Nikola Kojic, David L Kaplan, Annelise E Barron, Magali J Fontaine
Pancreatic islet encapsulation within biosynthetic materials has had limited clinical success due to loss of islet function and cell death. As an alternative encapsulation material, a silk-based scaffold was developed to reestablish the islet microenvironment lost during cell isolation. Islets were encapsulated with ECM proteins (laminin and collagen IV) and mesenchymal stromal cells (MSCs), known to have immunomodulatory properties or to enhance islet cell graft survival and function. After a 7 day in vitro encapsulation, islets remained viable and maintained insulin secretion in response to glucose stimulation...
October 2012: Biomaterials
https://www.readbyqxmd.com/read/22648809/divergent-dispersion-behavior-of-ssdna-fragments-during-microchip-electrophoresis-in-pdma-and-lpa-entangled-polymer-networks
#14
Christopher P Fredlake, Daniel G Hert, Thomas P Niedringhaus, Jennifer S Lin, Annelise E Barron
Resolution of DNA fragments separated by electrophoresis in polymer solutions ("matrices") is determined by both the spacing between peaks and the width of the peaks. Prior research on the development of high-performance separation matrices has been focused primarily on optimizing DNA mobility and matrix selectivity, and gave less attention to peak broadening. Quantitative data are rare for peak broadening in systems in which high electric field strengths are used (>150 V/cm), which is surprising since capillary and microchip-based systems commonly run at these field strengths...
May 2012: Electrophoresis
https://www.readbyqxmd.com/read/22506765/alginate-peg-sponge-architecture-and-role-in-the-design-of-insulin-release-dressings
#15
Michael Hrynyk, Manuela Martins-Green, Annelise E Barron, Ronald J Neufeld
Wound healing is a natural process involving several signaling molecules and cell types over a significant period of time. Although current dressings help to protect the wound from debris or infection, they do little in accelerating the healing process. Insulin has been shown to stimulate the healing of damaged skin. We have developed an alginate sponge dressing (ASD) that forms a hydrogel capable of providing a moist and protective healing environment. By incorporating insulin-loaded poly(d,l-lactide-co-glycolide) (PLGA) microparticles into ASD, we successfully stabilized and released insulin for up to 21 days...
May 14, 2012: Biomacromolecules
https://www.readbyqxmd.com/read/22486390/in-vivo-biodistribution-and-small-animal-pet-of-64-cu-labeled-antimicrobial-peptoids
#16
Jiwon Seo, Gang Ren, Hongguang Liu, Zheng Miao, Minyoung Park, Yihong Wang, Tyler M Miller, Annelise E Barron, Zhen Cheng
Peptoids are a rapidly developing class of biomimetic polymers based on oligo-N-substituted glycine backbones, designed to mimic peptides and proteins. Inspired by natural antimicrobial peptides, a group of cationic amphipathic peptoids has been successfully discovered with potent, broad-spectrum activity against pathogenic bacteria; however, there are limited studies to address the in vivo pharmacokinetics of the peptoids. Herein, (64)Cu-labeled DOTA conjugates of three different peptoids and two control peptides were synthesized and assayed in vivo by both biodistribution studies and small animal positron emission tomography (PET)...
May 16, 2012: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/22376164/synthesis-and-assembly-of-functional-high-molecular-weight-adiponectin-multimers-in-an-engineered-strain-of-escherichia-coli
#17
Sheng Ding, Daniel M Pinkas, Annelise E Barron
Adiponectin has many beneficial effects on cardiovascular and obesity-related disorders. It is part of a class of proteins that contains short collagenous domains, along with surfactant proteins A and D, and complement protein C1q. This class of biomacromolecules requires post-translational modifications to form biologically active assemblies. By introducing a set of post-translational modifying enzymes into Escherichia coli , we have created a prokaryotic expression system that functionally assembles adiponectin, as assessed by the ability of produced adiponectin multimers to suppress human endothelial cell apoptosis...
April 9, 2012: Biomacromolecules
https://www.readbyqxmd.com/read/22331820/quantitative-experimental-determination-of-primer-dimer-formation-risk-by-free-solution-conjugate-electrophoresis
#18
Samantha M Desmarais, Thomas Leitner, Annelise E Barron
DNA barcodes are short, unique ssDNA primers that "mark" individual biomolecules. To gain better understanding of biophysical parameters constraining primer-dimer formation between primers that incorporate barcode sequences, we have developed a capillary electrophoresis method that utilizes drag-tag-DNA conjugates to quantify dimerization risk between primer-barcode pairs. Results obtained with this unique free-solution conjugate electrophoresis approach are useful as quantitatively precise input data to parameterize computation models of dimerization risk...
February 2012: Electrophoresis
https://www.readbyqxmd.com/read/22290620/visualizing-and-quantifying-cell-phenotype-using-soft-x-ray-tomography
#19
REVIEW
Gerry McDermott, Douglas M Fox, Lindsay Epperly, Modi Wetzler, Annelise E Barron, Mark A Le Gros, Carolyn A Larabell
Soft X-ray tomography (SXT) is an imaging technique capable of characterizing and quantifying the structural phenotype of cells. In particular, SXT is used to visualize the internal architecture of fully hydrated, intact eukaryotic and prokaryotic cells at high spatial resolution (50 nm or better). Image contrast in SXT is derived from the biochemical composition of the cell, and obtained without the need to use potentially damaging contrast-enhancing agents, such as heavy metals. The cells are simply cryopreserved prior to imaging, and are therefore imaged in a near-native state...
April 2012: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/22180915/a-chemically-synthesized-peptoid-based-drag-tag-enhances-free-solution-dna-sequencing-by-capillary-electrophoresis
#20
Russell D Haynes, Robert J Meagher, Annelise E Barron
We report a capillary-based DNA sequencing read length of 100 bases in 16 min using end-labeled free-solution conjugate electrophoresis (FSCE) with a monodisperse poly-N-substituted glycine (polypeptoid) as a synthetic drag-tag. FSCE enabled rapid separation of single-stranded (ss) DNA sequencing fragments with single-base resolution without the need for a viscous DNA separation matrix. Protein-based drag-tags previously used for FSCE sequencing, for example, streptavidin, are heterogeneous in molar mass (polydisperse); the resultant band-broadening can make it difficult to obtain the single-base resolution necessary for DNA sequencing...
2011: Biopolymers
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