keyword
MENU ▼
Read by QxMD icon Read
search

Sphingolipid

keyword
https://www.readbyqxmd.com/read/28733363/lipidomic-profiling-of-patient-specific-induced-pluripotent-stem-cell-derived-hepatocyte-like-cells
#1
Mostafa Kiamehr, Leena E Viiri, Terhi Vihervaara, Kaisa M Koistinen, Mika Hilvo, Kim Ekroos, Reijo Käkelä, Katriina Aalto-Setälä
Hepatocyte-like cells (HLCs) differentiated from human induced pluripotent stem cells (iPSCs) offer an alternative model for primary human hepatocytes to study lipid aberrations. However, the detailed lipid profile of HLCs is yet unknown. In the current study, functional HLCs were differentiated from iPSCs generated from dermal fibroblasts of three individuals by a 3-step protocol through definitive endoderm (DE) stage. In parallel, detailed lipidomic analyses as well as gene expression profiling of a set of lipid metabolism-related genes were performed during the entire differentiation process from iPSC to HLCs...
July 21, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28733207/uplc-q-tof-ms-based-urine-and-plasma-metabonomics-study-on-the-ameliorative-effects-of-aspirin-eugenol-ester-in-hyperlipidemia-rats
#2
Ning Ma, Xi-Wang Liu, Xiao-Jun Kong, Zhe Qin, Shi-Hong Li, Zeng-Hua Jiao, Peng-Cheng Dong, Ya-Jun Yang, Jian-Yong Li
The main objective of this study was to investigate the ameliorative effects of aspirin eugenol ester (AEE) in hyperlipidemic rat. After five-week oral administration of AEE in high fat diet (HFD)-induced hyperlipidemic rats, the impact of AEE on plasma and urine metabonomics was investigated to explore the underlying mechanism by UPLC-Q-TOF/MS analysis. Blood lipid levels and histopathological changes of liver, stomach and duodenum were also evaluated after AEE treatment. Without obvious gastrointestinal (GI) side effects, AEE significantly relieved fatty degeneration of liver and reduced triglyceride (TG), low density lipoprotein (LDL) and total cholesterol (TCH) (P<0...
July 18, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28731533/improvement-of-d-lactic-acid-production-in-saccharomyces-cerevisiae-under-acidic-conditions-by-evolutionary-and-rational-metabolic-engineering
#3
Seung-Ho Baek, Eunice Y Kwon, Sang-Jeong Bae, Bo-Ram Cho, Seon-Young Kim, Ji-Sook Hahn
Microbial lactic acid (LA) production under acidic fermentation conditions is favorable to reduce the production cost, but circumventing LA toxicity is a major challenge. We generated D-LA-producing Saccharomyces cerevisiae strain JHY5610 by expressing D-lactate dehydrogenase gene (Lm. ldhA) from Leuconostoc mesenteroides, while deleting genes involved in ethanol production (ADH1, ADH2, ADH3, ADH4, and ADH5), glycerol production (GPD1 and GPD2), and degradation of D-LA (DLD1). Adaptive laboratory evolution of JHY5610 led to a strain JHY5710 having higher LA tolerance and D-LA-production capability...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28729700/sphingomimetic-multiple-sclerosis-drug-fty720-activates-vesicular-synaptobrevin-and-augments-neuroendocrine-secretion
#4
Frederic D Darios, Jernej Jorgacevski, Ajda Flašker, Robert Zorec, Virginia García-Martinez, José Villanueva, Luis M Gutiérrez, Charlotte Leese, Manjot Bal, Elena Nosyreva, Ege T Kavalali, Bazbek Davletov
Neurotransmission and secretion of hormones involve a sequence of protein/lipid interactions with lipid turnover impacting on vesicle trafficking and ultimately fusion of secretory vesicles with the plasma membrane. We previously demonstrated that sphingosine, a sphingolipid metabolite, promotes formation of the SNARE complex required for membrane fusion and also increases the rate of exocytosis in isolated nerve terminals, neuromuscular junctions, neuroendocrine cells and in hippocampal neurons. Recently a fungi-derived sphingosine homologue, FTY720, has been approved for treatment of multiple sclerosis...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729416/cib2-negatively-regulates-oncogenic-signaling-in-ovarian-cancer-via-sphingosine-kinase-1
#5
Wenying Zhu, Kate E Jarman, Noor A Lokman, Heidi A Neubauer, Lorena T Davies, Briony L Gliddon, Houng Taing, Paul A B Moretti, Martin K Oehler, Melissa R Pitman, Stuart M Pitson
Sphingosine kinase 1 (SK1) is a key regulator of the cellular balance between pro-apoptotic and pro-survival sphingolipids. Oncogenic signaling by SK1 relies on its localization to the plasma membrane, which is mediated by the calcium and integrin binding protein CIB1 via its Ca(2+)-myristoyl switch function. Here we show that another member of the CIB family, CIB2, plays a surprisingly opposite role to CIB1 in the regulation of SK1 signalling. CIB2 bound SK1 on the same site as CIB1, yet it lacks the Ca(2+)-myristoyl switch function...
July 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/28725570/enzyme-activities-of-%C3%AE-glucosidase-in-japanese-neonates-with-pseudodeficiency-alleles
#6
Ryuichi Mashima, Torayuki Okuyama
Lysosomal storage disorders (LSDs) are caused by defective enzyme activities in lysosomes, characterized by the accumulation of sphingolipids, glycolipids, oligosaccharides, mucopolysaccharides, the oxidation products of cholesterol, and other biological substances. A growing number of clinical studies have suggested the enhanced efficacy of existing therapies, including enzyme replacement therapy, which is effective when it is initiated during the presymptomatic period. Thus, the identification of disease-affected individuals by newborn screening has been considered an effective platform...
September 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28724695/caryolan-1-ol-an-antifungal-volatile-produced-by-streptomyces-spp-inhibits-the-endomembrane-system-of-fungi
#7
Gyeongjun Cho, Junheon Kim, Chung Gyoo Park, Corey Nislow, David M Weller, Youn-Sig Kwak
Streptomyces spp. have the ability to produce a wide variety of secondary metabolites that interact with the environment. This study aimed to discover antifungal volatiles from the genus Streptomyces and to determine the mechanisms of inhibition. Volatiles identified from Streptomyces spp. included three major terpenes, geosmin, caryolan-1-ol and an unknown sesquiterpene. antiSMASH and KEGG predicted that the volatile terpene synthase gene clusters occur in the Streptomyces genome. Growth inhibition was observed when fungi were exposed to the volatiles...
July 2017: Open Biology
https://www.readbyqxmd.com/read/28722418/urine-metabonomics-reveals-early-biomarkers-in-diabetic-cognitive-dysfunction
#8
Lili Song, Pengwei Zhuang, Mengya Lin, Mingqin Kang, Hongyue Liu, Yuping Zhang, Zhen Yang, Yunlong Chen, Yanjun Zhang
Recently, increasing attention has been paid to diabetic encephalopathy which is one of frequent diabetic complications and affects nearly 30% diabetics. Since cognitive dysfunction from diabetic encephalopathy might develop irreversible dementia, early diagnosis and detection of this disease is of great significance for its prevention and treatment. This study is to investigate the early specific metabolites biomarkers in the urine prior to the onset of diabetic cognitive dysfunction (DCD) by using metabolomics technology...
July 19, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28715731/metabolomics-analysis-reveals-that-benzo-a-pyrene-a-component-of-pm2-5-promotes-pulmonary-injury-by-modifying-lipid-metabolism-in-a-phospholipase-a2-dependent-manner-in-vivo-and-in-vitro
#9
Song-Yang Zhang, Danqing Shao, Huiying Liu, Juan Feng, Baihuan Feng, Xiaoming Song, Qian Zhao, Ming Chu, Changtao Jiang, Wei Huang, Xian Wang
Particulate matter with an aerodynamic diameter less than 2.5μM (PM2.5) is one of the major environmental pollutants in China. In this study, we carried out a metabolomics profile study on PM2.5-induced inflammation. PM2.5 from Beijing, China, was collected and given to rats through intra-tracheal instillation in vivo. Acute pulmonary injury were observed by pulmonary function assessment and H.E. staining. The lipid metabolic profile was also altered with increased phospholipid and sphingolipid metabolites in broncho-alveolar lavage fluid (BALF) after PM2...
July 4, 2017: Redox Biology
https://www.readbyqxmd.com/read/28714882/vitamin-d-supplementation-enhances-c18-dihydro-ceramide-levels-in-type-2-diabetes-patients
#10
Alexander Koch, Georgios Grammatikos, Sandra Trautmann, Yannick Schreiber, Dominique Thomas, Franziska Bruns, Josef Pfeilschifter, Klaus Badenhoop, Marissa Penna-Martinez
Sphingolipids are characterized by a broad range of bioactive properties. Particularly, the development of insulin resistance, a major pathophysiological hallmark of Type 2 Diabetes mellitus (T2D), has been linked to ceramide signaling. Since vitamin D supplementation may slow down T2D progression by improving glucose concentrations and insulin sensitivity, we investigated whether vitamin D supplementation impacts on plasma sphingolipid levels in T2D patients. Thus, plasma samples of 59 patients with non-insulin-requiring T2D from a placebo-controlled, randomized, and double-blind study were retrospectively analyzed...
July 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28714069/discriminant-biomarkers-of-acute-respiratory-distress-syndrome-associated-to-h1n1-influenza-identified-by-metabolomics-hplc-qtof-ms-ms-platform
#11
Alessia Ferrarini, Laura Righetti, Ma Paz Martínez, Mariano Fernández, Annalaura Mastrangelo, Juan P Horcajada, Antoni Betbesé, Andrés Esteban, Jordi Ordóñez, Joaquín de Gea, Jesús Ruiz Cabello, Federica Pellati, José A Lorente, Nicolás Nin, Francisco J Rupérez
Acute Respiratory Distress Syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis is unknown, and biomarkers are lacking. Untargeted metabolomics allows the analysis of the whole metabolome in a biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define the metabolic alterations occurring in patients with influenza A (H1N1) virus infection that developed ARDS...
July 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28711498/promotion-of-hair-growth-by-newly-synthesized-ceramide-mimetic-compound
#12
Bu-Mahn Park, Soon-Sun Bak, Kyung-Oh Shin, Minhee Kim, Daehwan Kim, Sang-Hun Jung, Sekyoo Jeong, Young Kwan Sung, Hyunjong Kim
Based on the crucial roles of ceramides in skin barrier function, use of ceramides or their structural mimetic compounds, pseudoceramides, as cosmetic ingredients are getting more popular. While currently used pseudoceramides are intended to substitute the structural roles of ceramides in stratum corneum, development of bioactive pseudoceramides has been repeatedly reported. In this study, based on the potential involvement of sphingolipids in hair cycle regulation, we investigated the effects of newly synthesized pseudoceramide, bis-oleamido isopropyl alcohol (BOI), on hair growth using cultured human hair follicles and animal models...
July 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28710072/circulating-acylcarnitine-profile-in-human-heart-failure-a-surrogate-of-fatty-acid-metabolic-dysregulation-in-mitochondria-and-beyond
#13
Matthieu Ruiz, Francois Labarthe, Annik Fortier, Bertrand Bouchard, Julie Thompson Legault, Virginie Bolduc, Odile Rigal, Jane Chen, Anique Ducharme, Peter A Crawford, Jean-Claude Tardif, Christine Des Rosiers
Heart failure (HF) is associated with metabolic perturbations, particularly of fatty acids (FA), which remain to be better understood in humans. This study aimed at testing the hypothesis that HF patients with reduced ejection fraction, display systemic perturbations in levels of energy-related metabolites, especially those reflecting dysregulation of FA metabolism, namely acylcarnitines (ACs). Circulating metabolites were assessed using mass spectrometry (MS)-based methods in two cohorts. The main cohort consisted of 72 controls and 68 HF patients, exhibiting depressed left ventricular ejection fraction (25...
July 14, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28709806/the-sugar-code-why-glycans-are-so-important
#14
REVIEW
Hans-Joachim Gabius
The cell surface is the platform for presentation of biochemical signals that are required for intercellular communication. Their profile necessarily needs to be responsive to internal and external factors in a highly dynamic manner. The structural features of the signals must meet the criterion of high-density information coding in a minimum of space. Thus, only biomolecules that can generate many different oligomers ('words') from few building blocks ('letters') qualify to meet this challenge. Examining the respective properties of common biocompounds that form natural oligo- and polymers comparatively, starting with nucleotides and amino acids (the first and second alphabets of life), comes up with sugars as clear frontrunner...
July 11, 2017: Bio Systems
https://www.readbyqxmd.com/read/28709768/plasma-sphingosine-1-phosphate-concentrations-are-associated-with-systolic-heart-failure-in-patients-with-ischemic-heart-disease
#15
Amin Polzin, Kerstin Piayda, Petra Keul, Lisa Dannenberg, Annemarie Mohring, Markus Gräler, Tobias Zeus, Malte Kelm, Bodo Levkau
OBJECTIVE: Sphingosine-1-Phosphate (S1P) is a bioactive sphingolipid with important functions in immunity, inflammation and cardiovascular biology. S1P is associated with prevalence and severity of coronary artery disease and myocardial infarction. However, its relevance in ischemic cardiomyopathy is unknown. We aimed to investigate associations of plasma S1P and other sphingolipids with the extent of heart failure in patients with ischemic heart disease. METHODS AND RESULTS: 74 patients with ischemic heart disease were investigated in this observational study...
July 12, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28706527/localization-of-sphingolipid-enriched-plasma-membrane-regions-and-long-chain-base-composition-during-mature-fruit-abscission-in-olive
#16
Maria C Parra-Lobato, Miguel A Paredes, Juana Labrador, Mariana Saucedo-García, Marina Gavilanes-Ruiz, Maria C Gomez-Jimenez
Sphingolipids, found in membranes of eukaryotic cells, have been demonstrated to carry out functions in various processes in plant cells. However, the roles of these lipids in fruit abscission remain to be determined in plants. Biochemical and fluorescence microscopy imaging approach has been adopted to investigate the accumulation and distribution of sphingolipids during mature-fruit abscission in olive (Olea europaea L. cv. Picual). Here, a lipid-content analysis in live protoplasts of the olive abscission zone (AZ) was made with fluorescent dyes and lipid analogs, particularly plasma membrane sphingolipid-enriched domains, and their dynamics were investigated in relation to the timing of mature-fruit abscission...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28701067/metabolomic-approach-to-understand-the-acute-and-chronic-hepatotoxicity-of-veratrum-nigrum-extract-in-mice-based-on-ultra-high-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#17
Ziheng Wei, Qingqing Qian, Xu Dong, Na Li, Guangchun Sun, Songyan Gao, Xin Dong
Veratrum nigrum L. (VN) is a poisonous traditional Chinese Medicine herb with thousands of years in China. Clinical studies have shown that VN has ability to cause hepatotoxicity, which severely limits its clinical use. The mechanism of its hepatotoxicity has not been fully elucidated. The purpose of this study was to develop and characterize a model of acute and chronic hepatotoxicity induced by Veratrum nigrum L. extract (VNE) to understand the mechanism by the liver tissue metabolomics approach based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOFMS)...
July 13, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28699577/sphingobacteriumsoli-sp-nov-isolated-from-soil
#18
Yu-Song Fu, Firasat Hussain, Neeli Habib, Inam Ullah Khan, Xiao Chu, Yan-Qing Duan, Xiao-Yang Zhi, Xing Chen, Wen-Jun Li
A Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated YIM X0211T, was isolated from a soil sample of Shiling County, Yunnan Province, south-west China. The new isolate was characterized taxonomically by using a polyphasic approach. The strain grew optimally at 30 °C, at pH 7.0 and with 0-3 % (w/v) NaCl. It was positive for catalase and oxidase but negative for H2S production. Comparative 16S rRNA gene sequence analysis showed that strain YIM X0211T fell within the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii DSM 11724T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28698382/phosphatidylserine-dictates-the-assembly-and-dynamics-of-caveolae-in-the-plasma-membrane
#19
Takashi Hirama, Raibatak Das, Yanbo Yang, Charles Ferguson, Amy Won, Christopher M Yip, Jason G Kay, Sergio Grinstein, Robert G Parton, Gregory D Fairn
Caveolae are bulb-shaped nanodomains of the plasma membrane that are enriched in cholesterol and sphingolipids. They have many physiological functions, including endocytic transport, mechanosensing, and regulation of membrane and lipid transport. Caveola formation relies on integral membrane proteins termed caveolins (CAVs) and the cavin family of peripheral proteins. Both protein families bind anionic phospholipids, but the precise roles of these lipids are unknown. Here, we studied the effects of phosphatidylserine (PtdSer), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2) on caveolar formation and dynamics...
July 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28698261/adipocyte-specific-deficiency-of-de-novo-sphingolipid-biosynthesis-leads-to-lipodystrophy-and-insulin-resistance
#20
Su-Yeon Lee, Hui-Young Lee, Jae-Hwi Song, Goon-Tae Kim, Suwon Jeon, Yoo-Jeong Song, Jae Sung Lee, Jang-Ho Hur, Hyun Hee Oh, Shi-Young Park, Soon-Mi Shim, Hyun Joo Yoo, Byung Cheon Lee, Xian-Cheng Jiang, Cheol Soo Choi, Tae-Sik Park
Sphingolipids have been implicated in the etiology of chronic metabolic diseases. Here, we investigated whether sphingolipid biosynthesis is associated with the development of adipose tissues and metabolic diseases. SPTLC2, a subunit of serine palmitoyltransferase, was transcriptionally upregulated in the adipose tissues of obese mice and in differentiating adipocytes. Adipocyte-specific SPTLC2-deficient (aSPTLC2 KO) mice had markedly reduced adipose tissue mass. Fatty acids that were destined for the adipose tissue were instead shunted to liver and caused hepatosteatosis...
July 11, 2017: Diabetes
keyword
keyword
44743
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"